Composition for inhibiting oxidation of horse oil and application thereof
By using antioxidants such as tea polyphenols, rosemary extract, rosemary leaf oil and other antioxidants in horse oil, the oxidation of horse oil is significantly inhibited, and the problems of poor stability and short shelf life of horse oil are solved, so as to achieve The long-term shelf life and high-quality storage of horse oil.
Patent Information
- Application Number
- CN202411564609.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-05
- Publication Date
- 2025-01-07
AI Technical Summary
Unsaturated fatty acids in horse oil are prone to oxidation, resulting in changes in oil quality and reduced nutritional value. Existing antioxidants cannot effectively prevent oxidation, resulting in poor stability of horse oil and short shelf life.
A composition is employed, including water-soluble antioxidants (such as tea polyphenols and rosemary extracts), fat-soluble antioxidants (such as rosemary leaf oil, tocopherol, tea polyphenol palmitate and ascorbyl palmitate ) and water-soluble antioxidant synergist (citric acid), significantly inhibit horse oil oxidation through enzymatic reaction and mixing processes.
It significantly extends the shelf life of horse oil, from 248 days to 927 days, maintains the color and odor of the oil, and reduces the peroxide value.
Smart Images
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Figure BDA0005118892240000102
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of horse oil preparation, and in particular relates to a composition for inhibiting the oxidation of horse oil and application thereof. Background Art
[0002] Oxidation of oils and fats can cause changes in oil quality characteristics, such as flavor, texture and consistency. In particular, unsaturated fatty acids in oils and fats are easily oxidized and produce hydroperoxides during processing and storage, and then decompose into compounds such as aldehydes and acids, and form pungent odors, thereby reducing the nutritional value of oils and fats and even endangering human health. The content of unsaturated fatty acids (UFAs) in horse oil is as high as 60%, so it is easy to oxidize and become rancid. In order to prevent horse oil from oxidizing and becoming rancid, maintain its quality and extend its shelf life, an appropriate amount of antioxidants is usually added to horse oil to prevent the occurrence of horse oil oxidation and delay the propagation of the oxidation chain. However, the current solution using antioxidants cannot effectively prevent the oxidation of horse oil, the stability of horse oil is poor, and the purpose of significantly increasing the shelf life of horse oil cannot be achieved. Summary of the invention
[0003] In view of this, one of the objects of the present invention is to provide a composition that can significantly inhibit the oxidation of horse oil, thereby significantly increasing the shelf life of horse oil.
[0004] A second object of the present invention is to provide a method for preparing horse oil using the composition of the present invention.
[0005] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:
[0006] The invention provides a composition for inhibiting the oxidation of horse oil. The composition comprises a water-soluble antioxidant, a fat-soluble antioxidant and a water-soluble antioxidant synergist; the water-soluble antioxidant comprises tea polyphenols and / or a water-soluble rosemary extract; the fat-soluble antioxidant comprises a fat-soluble antioxidant 1 and a fat-soluble antioxidant 2, the fat-soluble antioxidant 1 is rosemary leaf oil, and the fat-soluble antioxidant 2 comprises at least one of the following raw materials: tocopherol, tea polyphenol palmitate, ascorbyl palmitate and ascorbyl tetraisopalmitate; and the water-soluble antioxidant synergist is citric acid.
[0007] Preferably, the amount of tea polyphenols used is 0.01-0.05 g of tea polyphenols per 100 g of horse fat, based on the weight of horse fat; the amount of water-soluble rosemary extract used is 0.01-0.015 g of water-soluble rosemary extract per 100 g of horse fat, based on the weight of horse fat.
[0008] Preferably, the amount of the rosemary leaf oil used is 0.001 to 0.005 g of rosemary leaf oil per 100 g of horse fat, based on the weight of the horse fat.
[0009] Preferably, the amount of tocopherol used, calculated on the weight of horse oil, is 0.002-0.008 g of tocopherol per 100 g of horse oil; the amount of tea polyphenol palmitate used, calculated on the weight of horse oil, is 0.006-0.012 g of tea polyphenol palmitate per 100 g of horse oil; the amount of ascorbyl palmitate used, calculated on the weight of horse oil, is 0.006-0.012 g of ascorbyl palmitate per 100 g of horse oil; and the amount of ascorbyl tetraisopalmitate used, calculated on the weight of horse oil, is 0.005-0.01 g of ascorbyl tetraisopalmitate per 100 g of horse oil.
[0010] Preferably, the composition further comprises citric acid, and the amount of the citric acid used is 0.01 to 0.05 g of citric acid per 100 g of horse fat, based on the weight of the horse fat.
[0011] The invention also provides application of the composition in inhibiting oxidation of horse oil or prolonging the shelf life of horse oil.
[0012] The present invention also provides a method for preparing antioxidant horse oil using the above composition, comprising the following steps: mixing the water-soluble antioxidant, neutral protease and water to obtain a water-soluble substance; mixing the water-soluble substance with horse fat for enzymatic hydrolysis, and adding the rosemary leaf oil when the enzymatic hydrolysis reaction lasts 10-20 minutes; after the enzymatic hydrolysis reaction is completed, centrifuging to obtain a supernatant to obtain horse oil; mixing the horse oil with the fat-soluble antioxidant 2 to obtain antioxidant horse oil.
[0013] Preferably, the water-soluble substance also includes the above-mentioned water-soluble antioxidant synergist citric acid.
[0014] Preferably, the amount of the neutral protease is 150,000 to 250,000 U of neutral protease per 100 g of horse fat, based on the weight of horse fat; the weight ratio of horse fat to water is 1:1 to 3; the temperature of the enzymatic hydrolysis reaction is 45°C to 55°C, and the time of the enzymatic hydrolysis reaction is 1 to 2 hours.
[0015] Preferably, when the fat-soluble antioxidant 2 includes tea polyphenol palmitate or ascorbyl palmitate, tea polyphenol palmitate or ascorbyl palmitate is first dissolved in propylene glycol and then mixed with horse oil; the weight ratio of tea polyphenol palmitate to propylene glycol is 1:24-30; the weight ratio of ascorbyl palmitate to propylene glycol is 1:17-20.
[0016] Beneficial effects of the present invention:
[0017] The composition provided by the present invention can improve the stability of horse oil and significantly prolong the shelf life of horse oil, and can prolong the shelf life of horse oil from 248 days to 927 days. The horse oil prepared by the method of the present invention has uniform color, no rancidity, no bad smell and low peroxide value. DETAILED DESCRIPTION
[0018] The invention provides a composition for inhibiting the oxidation of horse oil. The composition comprises a water-soluble antioxidant, a fat-soluble antioxidant and a water-soluble antioxidant synergist; the water-soluble antioxidant comprises tea polyphenols and / or a water-soluble rosemary extract; the fat-soluble antioxidant comprises a fat-soluble antioxidant 1 and a fat-soluble antioxidant 2, the fat-soluble antioxidant 1 is rosemary leaf oil, and the fat-soluble antioxidant 2 comprises at least one of the following raw materials: tocopherol, tea polyphenol palmitate, ascorbyl palmitate and ascorbyl tetraisopalmitate; and the water-soluble antioxidant synergist is citric acid.
[0019] The present invention has no special limitation on the specific sources of the above raw materials, and any conventional commercial products in the art can be used. In the composition of the present invention, the amount of tea polyphenols is preferably 0.01-0.05g of tea polyphenols per 100g of horse fat, and more preferably 0.02-0.04g of tea polyphenols is added; the amount of water-soluble rosemary extract is preferably 0.01-0.015g of water-soluble rosemary extract per 100g of horse fat, and more preferably 0.012-0.014g of water-soluble rosemary extract is added.
[0020] In the composition of the present invention, the amount of rosemary leaf oil is preferably 0.001-0.005g of rosemary leaf oil per 100g of horse fat, and more preferably 0.002-0.004g of rosemary leaf oil is added. The amount of tocopherol is preferably 0.002-0.008g of tocopherol per 100g of horse oil, and more preferably 0.004-0.006g of tocopherol is added. The amount of tea polyphenol palmitate is preferably 0.006-0.012g of tea polyphenol palmitate per 100g of horse oil, and more preferably 0.008-0.01g of tea polyphenol palmitate is added. The amount of ascorbyl palmitate is preferably 0.006-0.012g of tea polyphenol palmitate per 100g of horse oil, and more preferably 0.008-0.01g of tea polyphenol palmitate is added. The amount used is calculated on the weight of horse oil, preferably 0.006-0.012 g of ascorbyl palmitate is added per 100 g of horse oil, and more preferably 0.008-0.01 g of ascorbyl palmitate is added; the amount of ascorbyl tetraisopalmitate is calculated on the weight of horse oil, preferably 0.005-0.01 g of ascorbyl tetraisopalmitate is added per 100 g of horse oil, and more preferably 0.006-0.008 g of ascorbyl tetraisopalmitate is added.
[0021] In the composition of the present invention, citric acid is preferably further included, and the amount of citric acid used is preferably 0.01-0.05 g of citric acid per 100 g of horse fat, and more preferably 0.02-0.04 g of citric acid per 100 g of horse fat. In the composition of the present invention, citric acid plays the role of an antioxidant synergist.
[0022] The invention also provides application of the composition in inhibiting oxidation of horse oil or prolonging the shelf life of horse oil.
[0023] The present invention also provides a method for preparing antioxidant horse oil using the above composition, comprising the following steps: mixing the water-soluble antioxidant, neutral protease and water to obtain a water-soluble substance; mixing the water-soluble substance with horse fat for enzymatic hydrolysis, and adding the rosemary leaf oil when the enzymatic hydrolysis reaction lasts 10-20 minutes; after the enzymatic hydrolysis reaction is completed, centrifuging to obtain a supernatant to obtain horse oil; mixing the horse oil with the fat-soluble antioxidant 2 to obtain antioxidant horse oil.
[0024] In the method of the present invention, the water-soluble substance preferably further comprises the above-mentioned citric acid. When the water-soluble substance comprises citric acid, the preparation process of the water-soluble substance is to mix a water-soluble antioxidant, citric acid, a neutral protease and water. In the present invention, the water is preferably distilled water.
[0025] In the method of the present invention, the amount of the neutral protease is based on the weight of horse fat, preferably 150,000 to 250,000 U of neutral protease is added per 100 g of horse fat, and more preferably 180,000 to 220,000 U of neutral protease is added per 100 g of horse fat; the weight ratio of horse fat to water is preferably 1:1 to 3, and more preferably 1:2; the temperature of the enzymatic hydrolysis reaction is preferably 45°C to 55°C, and more preferably 48°C to 52°C; the time of the enzymatic hydrolysis reaction is preferably 1 to 2 hours, and more preferably 1.2 to 1.8 hours. In the present invention, the centrifugal condition is preferably 4500 r / min for 5 minutes.
[0026] In the method of the present invention, when the fat-soluble antioxidant 2 includes tea polyphenol palmitate or ascorbyl palmitate, the tea polyphenol palmitate or ascorbyl palmitate is first dissolved in propylene glycol and then mixed with horse oil; the weight ratio of the tea polyphenol palmitate to propylene glycol is preferably 1:24-30, more preferably 1:26-28; the weight ratio of ascorbyl palmitate to propylene glycol is preferably 1:17-20, more preferably 1:18-19.
[0027] In the present invention, the composition is added in three steps during the enzymatic hydrolysis process to prepare horse oil, thereby achieving antioxidant properties in the entire process before, during and after the enzymatic hydrolysis reaction, greatly improving the antioxidant properties of horse oil, and extending the shelf life of horse oil from 248 days to 927 days.
[0028] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.
[0029] In the following embodiments, unless otherwise specified, all of them are conventional methods.
[0030] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.
[0031] In the following embodiments, tea polyphenols were purchased from Henan Sanhua Biotechnology Co., Ltd., citric acid was purchased from Guangzhou Jingke Reagent Co., Ltd., rosemary extract was purchased from Zhengzhou Xuanran Biotechnology Co., Ltd. The rosemary extract of this company is water-soluble and will be collectively referred to as water-soluble rosemary extract hereinafter, rosemary leaf oil was purchased from Guangzhou Wuzhitang Biotechnology Co., Ltd., tea polyphenol palmitate was purchased from Shuiquan Food Trading Co., Ltd., ascorbyl palmitate was purchased from Henan Senyuan Herbal Natural Products Co., Ltd., ascorbyl tetraisopalmitate was purchased from Guangzhou Yulu Chemical, and tocopherol was purchased from Guangzhou Fengting Chemical Co., Ltd.
[0032] Example 1
[0033] A composition for inhibiting the oxidation of horse oil, the composition consists of a water-soluble antioxidant and a fat-soluble antioxidant; the water-soluble antioxidant is 0.03g of tea polyphenols; the fat-soluble antioxidant consists of a fat-soluble antioxidant 1 and a fat-soluble antioxidant 2, the fat-soluble antioxidant 1 is 0.005g of rosemary leaf oil, the fat-soluble antioxidant 2 is tocopherol, and the weight ratio of tocopherol to horse oil is 0.005g:100g.
[0034] The method for preparing antioxidant horse oil by using the composition comprises the following steps: mixing 0.03g tea polyphenols, 200000U neutral protease and 100g water to obtain a water-soluble substance; mixing the water-soluble substance with 100g horse fat, stirring in a 50°C water bath for enzymolysis reaction for 1h, and adding 0.005g rosemary leaf oil after the enzymolysis reaction for 15min; after the enzymolysis reaction is completed, centrifuging at 4500r / min for 5min, and taking a supernatant to obtain horse oil; mixing tocopherol and horse oil at a weight ratio of 0.005g:100g to obtain antioxidant horse oil.
[0035] Example 2
[0036] A composition for inhibiting the oxidation of horse oil, the composition comprising a water-soluble antioxidant, a fat-soluble antioxidant and 0.04g of a water-soluble antioxidant synergist, citric acid; the water-soluble antioxidant is 0.012g of a water-soluble rosemary extract; the fat-soluble antioxidant comprises a fat-soluble antioxidant 1 and a fat-soluble antioxidant 2, the fat-soluble antioxidant 1 is 0.003g of rosemary leaf oil, the fat-soluble antioxidant 2 is ascorbyl palmitate, and the weight ratio of ascorbyl palmitate to horse oil is 0.006g:100g.
[0037] The method for preparing antioxidant horse oil by using the composition comprises the following steps: mixing 0.012g of water-soluble rosemary extract, 0.04g of citric acid, 200000U of neutral protease and 100g of water to obtain a water-soluble substance; mixing the water-soluble substance with 100g of horse fat, stirring in a 50°C water bath for enzymolysis reaction for 1h, and adding 0.003g of rosemary leaf oil after the enzymolysis reaction for 15min; after the enzymolysis reaction is completed, centrifuging at 4500r / min for 5min, and taking a supernatant to obtain horse oil; mixing ascorbyl palmitate with propylene glycol at a weight ratio of 1:17, and then mixing with horse oil to obtain antioxidant horse oil; the weight ratio of ascorbyl palmitate to horse oil is 0.006g:100g.
[0038] Example 3
[0039] A composition for inhibiting the oxidation of horse oil, the composition consisting of a water-soluble antioxidant and a fat-soluble antioxidant; the water-soluble antioxidant is 0.03g of tea polyphenols and 0.012g of water-soluble rosemary extract; the fat-soluble antioxidant consists of a fat-soluble antioxidant 1 and a fat-soluble antioxidant 2, the fat-soluble antioxidant 1 is 0.003g of rosemary leaf oil, the fat-soluble antioxidant 2 is tocopherol and tea polyphenol palmitate, the weight ratio of tocopherol to horse oil is 0.002g:100g, and the weight ratio of tea polyphenol palmitate to horse oil is 0.009g:100g.
[0040] The method for preparing antioxidant horse oil by using the composition comprises the following steps: mixing 0.03g tea polyphenols, 0.012g water-soluble rosemary extract, 250000U neutral protease and 300g water to obtain a water-soluble substance; mixing the water-soluble substance with 100g horse fat, stirring in a water bath at 55°C for enzymolysis reaction for 2h, and adding 0.003g rosemary leaf oil when the enzymolysis reaction lasts for 15min; after the enzymolysis reaction is completed, centrifuging at 4500r / min for 5min, and taking a supernatant to obtain horse oil; mixing tea polyphenol palmitate with propylene glycol at a weight ratio of 1:24, and then mixing with tocopherol and horse oil to obtain antioxidant horse oil; wherein the weight ratio of tocopherol to horse oil is 0.002g:100g, and the weight ratio of tea polyphenol palmitate to horse oil is 0.009g:100g.
[0041] Example 4
[0042] A composition for inhibiting the oxidation of horse oil, the composition consisting of a water-soluble antioxidant and a fat-soluble antioxidant; the water-soluble antioxidant is 0.03g of tea polyphenols and 0.012g of water-soluble rosemary extract; the fat-soluble antioxidant consists of a fat-soluble antioxidant 1 and a fat-soluble antioxidant 2, the fat-soluble antioxidant 1 is 0.003g of rosemary leaf oil, the fat-soluble antioxidant 2 is tocopherol and tea polyphenol palmitate, the weight ratio of tocopherol to horse oil is 0.002g:100g, and the weight ratio of tea polyphenol palmitate to horse oil is 0.009g:100g.
[0043] The method for preparing antioxidant horse oil by using the composition comprises the following steps: mixing 0.03g tea polyphenols, 0.012g water-soluble rosemary extract, 200000U neutral protease and 100g water to obtain a water-soluble substance; mixing the water-soluble substance with 100g horse fat, stirring in a 50°C water bath for enzymolysis reaction for 1h, and adding 0.003g rosemary leaf oil when the enzymolysis reaction lasts for 15min; after the enzymolysis reaction is completed, centrifuging at 4500r / min for 5min, and taking a supernatant to obtain horse oil; mixing tea polyphenol palmitate with propylene glycol at a weight ratio of 1:24, and then mixing with tocopherol and horse oil to obtain antioxidant horse oil; wherein the weight ratio of tocopherol to horse oil is 0.002g:100g, and the weight ratio of tea polyphenol palmitate to horse oil is 0.009g:100g.
[0044] Example 5
[0045] A composition for inhibiting the oxidation of horse oil, the composition consists of a water-soluble antioxidant, a fat-soluble antioxidant and 0.01g of a water-soluble antioxidant synergist, citric acid; the water-soluble antioxidant is 0.01g of tea polyphenols and 0.01g of a water-soluble rosemary extract; the fat-soluble antioxidant consists of a fat-soluble antioxidant 1 and a fat-soluble antioxidant 2, the fat-soluble antioxidant 1 is 0.001g of rosemary leaf oil, the fat-soluble antioxidant 2 is tocopherol, tea polyphenol palmitate and ascorbyl palmitate, the weight ratio of tocopherol to horse oil is 0.002g:100g, the weight ratio of tea polyphenol palmitate to horse oil is 0.006g:100g, and the weight ratio of ascorbyl palmitate to horse oil is 0.006g:100g.
[0046] The method for preparing antioxidant horse oil by using the composition comprises the following steps: mixing 0.01g of tea polyphenols, 0.01g of water-soluble rosemary extract, 0.01g of citric acid, 200000U of neutral protease and 100g of water to obtain a water-soluble substance; mixing the water-soluble substance with 100g of horse fat, stirring in a 50°C water bath for enzymolysis reaction for 1h, adding 0.001g of rosemary leaf oil when the enzymolysis reaction lasts for 15min; after the enzymolysis reaction is completed, centrifuging at 4500r / min for 5min, taking the supernatant as horse oil; mixing tea polyphenol palmitate with propylene glycol according to The invention relates to a method for preparing an antioxidant horse oil by mixing tea polyphenol palmitate in a weight ratio of 1:24 to obtain a tea polyphenol palmitate solution, mixing ascorbyl palmitate and propylene glycol in a weight ratio of 1:20 to obtain an ascorbyl palmitate solution, and then mixing the tea polyphenol palmitate solution, ascorbyl palmitate solution, tocopherol and horse oil to obtain antioxidant horse oil; wherein the weight ratio of tocopherol to horse oil is 0.002g:100g, the weight ratio of tea polyphenol palmitate to horse oil is 0.006g:100g, and the weight ratio of ascorbyl palmitate to horse oil is 0.006g:100g.
[0047] Example 6
[0048] A composition for inhibiting the oxidation of horse oil, the composition consists of a water-soluble antioxidant, a fat-soluble antioxidant and 0.05g of a water-soluble antioxidant synergist, citric acid; the water-soluble antioxidant is 0.05g of tea polyphenols and 0.015g of a water-soluble rosemary extract; the fat-soluble antioxidant consists of a fat-soluble antioxidant 1 and a fat-soluble antioxidant 2, the fat-soluble antioxidant 1 is 0.005g of rosemary leaf oil, the fat-soluble antioxidant 2 is tocopherol, tea polyphenol palmitate and ascorbyl palmitate, the weight ratio of tocopherol to horse oil is 0.002g:100g, the weight ratio of tea polyphenol palmitate to horse oil is 0.01g:100g, and the weight ratio of ascorbyl palmitate to horse oil is 0.012g:100g.
[0049] The method for preparing antioxidant horse oil by using the composition comprises the following steps: mixing 0.05g tea polyphenols, 0.015g water-soluble rosemary extract, 0.05g citric acid, 200000U neutral protease and 100g water to obtain a water-soluble substance; mixing the water-soluble substance with 100g horse fat, stirring in a 50°C water bath for enzymolysis reaction for 1h, adding 0.005g rosemary leaf oil when the enzymolysis reaction lasts for 15min; after the enzymolysis reaction is completed, centrifuging at 4500r / min for 5min, taking the supernatant as horse oil; mixing tea polyphenol palmitate with propylene glycol according to Mix them in a weight ratio of 1:24 to obtain a tea polyphenol palmitate solution, mix ascorbyl palmitate and propylene glycol in a weight ratio of 1:20 to obtain an ascorbyl palmitate solution, and then mix the tea polyphenol palmitate solution, ascorbyl palmitate solution, tocopherol and horse oil to obtain antioxidant horse oil; wherein the weight ratio of tocopherol to horse oil is 0.002g:100g, the weight ratio of tea polyphenol palmitate to horse oil is 0.01g:100g, and the weight ratio of ascorbyl palmitate to horse oil is 0.012g:100g.
[0050] Example 7
[0051] A composition for inhibiting the oxidation of horse oil, the composition consists of a water-soluble antioxidant, a fat-soluble antioxidant and 0.04g of a water-soluble antioxidant synergist, citric acid; the water-soluble antioxidant is 0.03g of tea polyphenols and 0.012g of a water-soluble rosemary extract; the fat-soluble antioxidant consists of a fat-soluble antioxidant 1 and a fat-soluble antioxidant 2, the fat-soluble antioxidant 1 is 0.003g of rosemary leaf oil, the fat-soluble antioxidant 2 is tocopherol, tea polyphenol palmitate and ascorbyl palmitate, the weight ratio of tocopherol to horse oil is 0.005g:100g, the weight ratio of tea polyphenol palmitate to horse oil is 0.009g:100g, and the weight ratio of ascorbyl palmitate to horse oil is 0.008g:100g.
[0052] The method for preparing antioxidant horse oil by using the composition comprises the following steps: mixing 0.03g tea polyphenols, 0.012g water-soluble rosemary extract, 0.04g citric acid, 200000U neutral protease and 100g water to obtain a water-soluble substance; mixing the water-soluble substance with 100g horse fat, stirring in a 50°C water bath for enzymolysis reaction for 1h, adding 0.003g rosemary leaf oil when the enzymolysis reaction lasts for 15min; after the enzymolysis reaction is completed, centrifuging at 4500r / min for 5min, taking the supernatant as horse oil; mixing tea polyphenol palmitate with propylene glycol according to Mix them in a weight ratio of 1:24 to obtain a tea polyphenol palmitate solution, mix ascorbyl palmitate and propylene glycol in a weight ratio of 1:20 to obtain an ascorbyl palmitate solution, and then mix the tea polyphenol palmitate solution, ascorbyl palmitate solution, tocopherol and horse oil to obtain antioxidant horse oil; wherein the weight ratio of tocopherol to horse oil is 0.005g:100g, the weight ratio of tea polyphenol palmitate to horse oil is 0.009g:100g, and the weight ratio of ascorbyl palmitate to horse oil is 0.008g:100g.
[0053] Example 8
[0054] A composition for inhibiting the oxidation of horse oil, the composition consists of a water-soluble antioxidant, a fat-soluble antioxidant and 0.04g of a water-soluble antioxidant synergist, citric acid; the water-soluble antioxidant is 0.03g of tea polyphenols and 0.012g of a water-soluble rosemary extract; the fat-soluble antioxidant consists of a fat-soluble antioxidant 1 and a fat-soluble antioxidant 2, the fat-soluble antioxidant 1 is 0.003g of rosemary leaf oil, the fat-soluble antioxidant 2 is tocopherol, tea polyphenol palmitate and ascorbyl tetraisopalmitate, the weight ratio of tocopherol to horse oil is 0.005g:100g, the weight ratio of tea polyphenol palmitate to horse oil is 0.009g:100g, and the weight ratio of ascorbyl tetraisopalmitate to horse oil is 0.008g:100g.
[0055] The method for preparing antioxidant horse oil using the composition comprises the following steps: mixing 0.03g tea polyphenols, 0.012g water-soluble rosemary extract, 0.04g citric acid, 200000U neutral protease and 100g water to obtain a water-soluble substance; mixing the water-soluble substance with 100g horse fat, stirring in a 50°C water bath for enzymolysis reaction for 1h, adding 0.003g rosemary leaf oil when the enzymolysis reaction lasts for 15min; after the enzymolysis reaction is completed, centrifuging at 4500r / min for 5min, and taking the supernatant. liquid, which is horse oil; tea polyphenol palmitate and propylene glycol are mixed in a weight ratio of 1:24 to obtain a tea polyphenol palmitate solution, and then the tea polyphenol palmitate solution, ascorbyl tetraisopalmitate, tocopherol and horse oil are mixed to obtain antioxidant horse oil; wherein the weight ratio of tocopherol to horse oil is 0.005g:100g, the weight ratio of tea polyphenol palmitate to horse oil is 0.009g:100g, and the weight ratio of ascorbyl tetraisopalmitate to horse oil is 0.008g:100g.
[0056] Comparative Example 1
[0057] A kind of horse oil, the horse oil is prepared by the following preparation method: 200000U neutral protease and 100g water are mixed to obtain a water-soluble substance; the water-soluble substance is mixed with 100g horse fat, and the enzymatic hydrolysis reaction is stirred in a 50°C water bath for 1h. After the enzymatic hydrolysis reaction is completed, the mixture is centrifuged at 4500r / min for 5min, and the supernatant is taken as the horse oil.
[0058] Comparative Example 2
[0059] The difference from Example 7 is that the fat-soluble antioxidant component is not contained, and the rest is the same as Example 7.
[0060] That is: a composition for inhibiting the oxidation of horse oil, the composition consists of a water-soluble antioxidant and 0.04g of a water-soluble antioxidant synergist, citric acid; the water-soluble antioxidant is 0.03g of tea polyphenols and 0.012g of a water-soluble rosemary extract.
[0061] The method for preparing antioxidant horse oil by using the composition comprises the following steps: 0.03g tea polyphenols, 0.012g water-soluble rosemary extract, 0.04g citric acid, 200000U neutral protease and 100g water are mixed to obtain a water-soluble substance; the water-soluble substance is mixed with 100g horse fat, and enzymolysis reaction is carried out by stirring in a 50°C water bath for 1h. After the enzymolysis reaction is completed, centrifugation is performed at 4500r / min for 5min, and a supernatant is taken to obtain the horse oil.
[0062] Comparative Example 3
[0063] The invention discloses an antioxidant horse oil, which is prepared by the following preparation method: mixing 200000U neutral protease and 100g water to obtain a water-soluble substance; mixing the water-soluble substance with 100g horse fat, stirring in a 50°C water bath for enzymolysis reaction for 1h, adding 0.003g rosemary leaf oil when the enzymolysis reaction lasts for 15min; after the enzymolysis reaction is completed, centrifuging at 4500r / min for 5min, and taking a supernatant to obtain the antioxidant horse oil.
[0064] Comparative Example 4
[0065] The invention discloses an antioxidant horse oil, which is prepared by the following preparation method: mixing 200000U neutral protease and 100g water to obtain a water-soluble substance; mixing the water-soluble substance with 100g horse fat, stirring in a water bath at 55°C for enzymolysis reaction for 1h, centrifuging at 4500r / min for 5min after the enzymolysis reaction is completed, and taking the supernatant as horse oil; mixing tea polyphenol palmitate with propylene glycol in a weight ratio of 1:24 to obtain a tea polyphenol palmitate solution, and then mixing the tea polyphenol palmitate solution, ascorbyl tetraisopalmitate, tocopherol and horse oil to obtain the antioxidant horse oil; wherein the weight ratio of tocopherol to horse oil is 0.005g:100g, the weight ratio of tea polyphenol palmitate to horse oil is 0.009g:100g, and the weight ratio of ascorbyl tetraisopalmitate to horse oil is 0.008g:100g.
[0066] Comparative Example 5
[0067] The invention discloses an antioxidant horse oil, which is prepared by the following preparation method: weighing 0.03g of tea polyphenols, 0.012g of water-soluble rosemary extract and 0.04g of water-soluble antioxidant synergist citric acid, mixing them with 200000U of neutral protease and 100g of water to obtain a water-soluble substance; mixing the water-soluble substance with 100g of horse fat, stirring in a 50°C water bath for enzymolysis reaction for 1h, adding 0.003g of rosemary leaf oil when the enzymolysis reaction lasts for 15min; after the enzymolysis reaction is completed, centrifuging at 4500r / min for 5min, and taking the supernatant to obtain the antioxidant horse oil.
[0068] Example 9
[0069] Odor evaluation: A professionally trained evaluation team of 10 people was selected to evaluate the odor of the horse oil obtained in Examples 1 to 8 and Comparative Examples 1 to 5 according to the scoring criteria in Table 1. The team members evaluated the samples one by one, rested for 1 minute after completing the evaluation of one sample, and then evaluated the next sample. The results were calculated as the average value.
[0070] Table 1 Horse oil scoring standards
[0071]
[0072]
[0073] Determination of horse oil peroxide value and prediction of shelf life:
[0074] The peroxide value of the horse oil obtained in Examples 1 to 8 and Comparative Examples 1 to 5 was measured according to GB5009.227-2016 "Determination of Peroxide Value in Foods - National Food Safety Standard"
[0075] The Schaal oven method was used to conduct experiments to predict the shelf life of the horse oil obtained in Examples 1 to 8 and Comparative Examples 1 to 5. The specific method is as follows: the horse oil was weighed, placed in a brown wide-mouth bottle, sealed and placed in an oven at 4, 25, 35, 45, and 55°C, the sample was stirred every other day to ensure that it was evenly heated, and the sample was placed for 49 days, and the peroxide value was measured every 7 days. The first-order reaction kinetic equation (1) at different storage temperatures was fitted with the storage time as the horizontal coordinate and POV as the vertical coordinate to obtain the reaction rate constant K. The Arrhenius equation was deformed to obtain formula (2), and both sides of formula (2) were taken to obtain formula (3). The rate constant K at different temperatures obtained by formula (1) was substituted into formula (3), and a straight line equation was obtained with 1 / T as the horizontal coordinate and lnK as the vertical coordinate. Its slope was (-Ea / R) and its intercept was lnK0, thereby obtaining the reaction activation energy Ea and K0. Taking the limit POV≤0.20g / 100g of GB10146-2015 "National Food Safety Standard Edible Animal Fats and Oils" as the limit value, substitute the obtained Ea and K0 into formula (2) to obtain K of horse oil at a storage temperature of 8℃, and substitute it into formula (1) to calculate the shelf life of horse oil at 8℃.
[0076] B=B0e Kt (1)
[0077] Where: B is the POV of horse oil on the tth day of storage (g / 100g); B0 is the initial POV of horse oil (g / 100g); K is the rate constant of change of horse oil POV (g / 100g) corresponding to storage time; t is the storage time, d.
[0078]
[0079] Where: Ea is the apparent activation energy kJ / mol; K is the rate constant of change of horse oil POV (g / 100g); K0 represents the frequency factor (prefactor) of the equation; T represents the absolute temperature; R represents the gas constant of 8.314 J / (mol·K).
[0080] The results of the smell, peroxide value and shelf life of the horse oil are shown in Table 2. The results show that the horse oil obtained in the comparative example without adding the composition of the present invention has an odor and a rancid smell, while the horse oil obtained in the example with the composition of the present invention has no odor and no rancidity. The shelf life of the horse oil prepared without adding any antioxidant at 8°C is 248 days, and the shelf life of the horse oil with the composition of the present invention is 718 to 927 days, which is 2.8 to 3.7 times that of the comparative example.
[0081] Table 2 Results of different horse oil odors, peroxide values and shelf life
[0082]
[0083] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.
Claims
1. A composition for inhibiting oxidation of horse oil, characterized in that: The composition comprises a water-soluble antioxidant and a fat-soluble antioxidant; the water-soluble antioxidant comprises tea polyphenols and / or a water-soluble rosemary extract; the fat-soluble antioxidant comprises a fat-soluble antioxidant 1 and a fat-soluble antioxidant 2, the fat-soluble antioxidant 1 is rosemary leaf oil, and the fat-soluble antioxidant 2 comprises at least one of the following raw materials: tocopherol, tea polyphenol palmitate, ascorbyl palmitate and ascorbyl tetraisopalmitate.
2. The composition according to claim 1, characterized in that The amount of the tea polyphenols is calculated based on the weight of horse fat, and is 0.01-0.05g of tea polyphenols is added per 100g of horse fat; the amount of the water-soluble rosemary extract is calculated based on the weight of horse fat, and is 0.01-0.015g of water-soluble rosemary extract is added per 100g of horse fat.
3. The composition according to claim 1, characterized in that The dosage of the rosemary leaf oil is calculated based on the weight of horse fat, that is, 0.001-0.005 g of rosemary leaf oil is added to every 100 g of horse fat.
4. The composition according to claim 1, characterized in that The amount of tocopherol used is 0.002-0.008g of tocopherol per 100g of horse oil, based on the weight of horse oil; the amount of tea polyphenol palmitate used is 0.006-0.012g of tea polyphenol palmitate per 100g of horse oil; the amount of ascorbyl palmitate used is 0.006-0.012g of ascorbyl palmitate per 100g of horse oil; the amount of ascorbyl tetraisopalmitate used is 0.005-0.01g of ascorbyl tetraisopalmitate per 100g of horse oil, based on the weight of horse oil.
5. The composition according to any one of claims 1 to 4, characterized in that The composition also includes citric acid, and the amount of the citric acid used is 0.01-0.05 g of citric acid per 100 g of horse fat, based on the weight of the horse fat.
6. Use of the composition according to any one of claims 1 to 5 for inhibiting oxidation of horse oil or extending the shelf life of horse oil.
7. A method for preparing antioxidant horse oil using the composition according to any one of claims 1 to 4, characterized in that: The method comprises the following steps: mixing the water-soluble antioxidant, neutral protease and water to obtain a water-soluble substance; mixing the water-soluble substance with horse fat for enzymolysis reaction, and adding the rosemary leaf oil when the enzymolysis reaction lasts for 10-20 minutes; after the enzymolysis reaction is completed, centrifuging and taking the supernatant to obtain horse oil; and mixing the horse oil with the fat-soluble antioxidant 2 to obtain antioxidant horse oil.
8. The method according to claim 7, characterized in that The water-soluble substance also includes the citric acid according to claim 5.
9. The method according to claim 7, characterized in that: The dosage of the neutral protease is calculated based on the weight of horse fat, that is, 150,000 to 250,000 U of neutral protease is added for every 100 g of horse fat; the weight ratio of horse fat to water is 1:1 to 3; the temperature of the enzymatic hydrolysis reaction is 45°C to 55°C, and the time of the enzymatic hydrolysis reaction is 1 to 2 hours.
10. The method according to claim 7, characterized in that When the fat-soluble antioxidant 2 includes tea polyphenol palmitate or ascorbyl palmitate, tea polyphenol palmitate or ascorbyl palmitate is first dissolved in propylene glycol and then mixed with horse oil; the weight ratio of tea polyphenol palmitate to propylene glycol is 1:24-30; the weight ratio of ascorbyl palmitate to propylene glycol is 1:17-20.