Device and method for feeding adult thrips megalurothrips

Abstract

The larva rearing device is characterized in that a box body is divided into an upper box chamber and a lower box chamber, the upper box chamber and the lower box chamber are separated by a partition plate with holes, the upper layer is an adult rearing chamber, and the lower layer is a glass larva rearing tank containing chamber; the top layer and the two sides of the side edges are provided with ventilation opening gauze covering nets, and the front face is a detachable box door with an operation window; in the feeding process, insect bodies are not directly contacted in the whole process, death caused by physical damage to the insect bodies is reduced, the adult day age or the nymph age of the megalurothrips megalurothrips can be accurately mastered, and the development condition of the megalurothrips megalurothrips in each age can be observed. The method is simple and convenient to operate, and a large number of insect source materials with accurate and reliable ages can be provided for research of the megalustris megalurothrips.

Description

Technical Field

[0001] The invention belongs to the biological field and relates to an indoor breeding device and a breeding method for Thrips leucophylla. Background Art

[0002] Thrips pea Megalurothrips usitatus (Bagnall) Thysanoptera Thripidae Thripidae Megalurothrips There are 28 host plants of thrips, most of which are leguminous crops. They can damage the flowers, leaves and pods of crops. thrips mainly occur in large numbers in cowpea producing areas, causing cowpea yields to decrease by 20%-30% in mild cases, 50%-80% in severe cases, or even total failure.

[0003] In view of the importance of Thrips beanensis, it is urgent to establish an indoor population of Thrips beanensis and carry out relevant research on its living habits and drug resistance. The previous breeding method is cumbersome to operate, the breeding conditions are difficult to control, the thrips are easy to escape, and it is difficult to observe the breeding situation of Thrips beanensis, and the population is difficult to expand. The present invention makes a device for breeding adults of Thrips beanensis and establishes a breeding method, which provides a basis for indoor research on Thrips beanensis. Summary of the invention

[0004] The main purpose of the invention is to realize the mass breeding of giant bean thrips and accurately grasp the development stage of giant bean thrips.

[0005] In order to achieve the purpose of the present invention, a breeding device for Thrips leucoderma was first designed:

[0006] The insect breeding box is divided into two chambers, upper and lower, separated by a partition with holes. The upper chamber is the adult breeding chamber, and the lower chamber is the glass insect jar placement chamber. The top layer and the sides are provided with vents covered with gauze, and the front is a detachable door with an operating window.

[0007] Preferably, the insect breeding box body is made of a 1 cm thick acrylic plate, with dimensions of 43 cm in length, 35 cm in width and 43 cm in height.

[0008] Preferably, the air permeable opening has a size of 30 cm × 30 cm, and the gauze has a mesh size of 200.

[0009] Preferably, the acrylic partition has a thickness of 0.5 cm, a size of 41 cm × 33 cm, and is provided with 36 (6 × 6) holes with a diameter of 2 cm.

[0010] Preferably, the upper chamber is 18 cm high and the lower chamber is 24 cm high.

[0011] Preferably, a magnet sheet with a diameter of 1 cm is embedded in each corner of the box body and the box door, so that the box body and the box door are connected by magnetic attraction.

[0012] Preferably, the operating window on the box door has a size of 15 cm × 10 cm, and a 0.5 cm magnet is embedded in the window cover and the window, so that the window cover and the box door window are connected by magnetic attraction, and the window cover has an opening of 13 cm × 8 cm and is covered with a 200 mesh gauze.

[0013] The present invention also uses the above device to establish a method for indoor breeding of Macrothrips leucoderma, and the specific steps are as follows:

[0014] (1) Transfer the adult thrips into the insect box and place the whole lentil pods on the upper layer of the insect box and lay them flat on the partition for the adult thrips to feed and lay eggs;

[0015] (2) After 1-2 days (before the eggs hatch), insert the CO2 gun into the operating window to blow the bean thrips off the pods and drop them into the box. Take out the pods that have been feeding on the eggs and put in fresh pods.

[0016] (3) Place the bean pods after feeding and laying eggs in a glass insect jar, wait for the eggs to hatch into nymphs, and continue to raise them;

[0017] (4) After the adults emerge from their pupae, open the glass insect jar and place it directly into the lower layer of the insect box. Wait for the adults to gather on the fresh bean pods on the upper layer and continue to feed and lay eggs.

[0018] Preferably, 10-15 lentil pods are placed in each box, which can accommodate 500-4000 adult bean thrips.

[0019] Preferably, 8-10 wrinkled napkins are placed at the bottom of the glass insect jar to provide a pupation environment for the thrips.

[0020] Preferably, 5 bean pods after feeding and laying eggs are placed in the glass insect jar to ensure the humidity in the insect jar.

[0021] Preferably, the environmental conditions of the breeding method are a temperature of 25 ± 5 °C, a relative humidity of 60%-70%, and a light intensity of 16L:8L.

[0022] The beneficial effects of the present invention are:

[0023] 1. This breeding process is easy to operate and does not directly touch the insect body, which can avoid frequent changes of hosts or frequent use of brushes and other tools to pick insects and cause population losses;

[0024] 2. The insect sources in this breeding process are uniform in size, and the age of adults or nymphs of Thrips leucoderma can be accurately grasped, and insects of appropriate age can be selected for relevant research;

[0025] 3. The feeding device has a simple structure and is made of transparent material, which is convenient for observing the population and development of Thrips Beani. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 This is a schematic diagram of the structure of the breeding box for adults of Thrips fasciatus.

[0027] In the picture: 1. Magnet 2. Window 3. Door 4. Ventilation 5. Partition DETAILED DESCRIPTION

[0028] The breeding scheme of Macrothrips granatum is described in detail below with reference to specific embodiments.

[0029] Materials needed for breeding: homemade insect boxes, insect jars, kitchen paper, napkins, sealing film, wide tape, carbon dioxide air gun, and lentil pods.

[0030] The breeding steps of Thrips leucoderma of the present invention are as follows:

[0031] (1) Place 10-15 clean lentil pods flat on the upper layer of the insect box, place 1,000-4,000 adult thrips in the box, and seal the connection between the box door and the box with wide tape to prevent the thrips from escaping.

[0032] (2) Use wide tape to seal the connection between the box body and the door of the insect box to prevent thrips from escaping.

[0033] (3) After one day, insert the carbon dioxide air gun into the operating window to blow the bean thrips off the pods and drop them into the box. Then, take out the pods used for feeding and laying eggs from the box and replace them with new cleaned pods.

[0034] (4) Rinse the pods taken out of the box with clean water several times and wipe them dry to avoid bacteria or predatory mites;

[0035] (5) Prepare an insect jar and put 8-10 wrinkled napkins in it to provide a suitable place for thrips to pupate;

[0036] (6) Place five bean pods that have been fed and laid eggs vertically into a prepared insectary jar on top of toilet paper;

[0037] (7) The lid of the glass insect jar (12 cm) should have a 10 cm ventilation hole and be covered with a 200 mesh breathable gauze.

[0038] (8) Stretch a 2 cm wide and 10 cm long sealing film longitudinally and wrap it around the thread of the mouth of the insect jar. Tighten the lid. Then wrap a sealing film of the same specification around the connection between the mouth of the jar and the lid to prevent the thrips from escaping.

[0039] (9) After completing the above operations, mark the population number, breeding generation and transfer date on the glass jar, and avoid opening the lid before eclosion;

[0040] (10) After a large number of bean thrips have emerged as adults, open the glass jar and move it to the lower layer of the insect box. The adults will fly from the glass jar to the upper layer of the bean pods and continue to feed and lay eggs.

[0041] According to the content of the invention, the life span of adults of Thrips pea, the number of adults emerging after egg laying and collection, and the ratio of male to female were tested. 500 Thrips pea were reared in each box, repeated 3 times, and the whole-process rearing method of insect jar was used as a control, and the relevant parameters were counted until the end of a complete generation.

[0042] Statistical results show that the average lifespan of the bean thrips adults raised by the present invention is 16.61 days, which is longer than the 7.34 days of those raised in insect jars; after taking out the beans after laying eggs one day later, the average number of adults that can emerge is 1303.7, with a female-to-male ratio of 3.95:1, which is much higher than the 544 adults raised in insect jars throughout the whole process, with a female-to-male ratio of 1.77:1.

Claims

1. A device for rearing adults of Thrips leucophylla, characterized in that: The insect breeding box is divided into two chambers, upper and lower, separated by a partition with holes. The upper chamber is the adult breeding chamber, and the lower chamber is the glass insect jar placement chamber. The top layer and the sides are provided with vents covered with gauze, and the front is a detachable door with an operating window.

2. A device for rearing adults of Thrips leucocephalus according to claim 1, characterized in that: The insect breeding box body is made of a 1 cm thick acrylic plate, and the dimensions are 43 cm long, 35 cm wide, and 43 cm high.

3. A device for rearing adults of Thrips leucophylla according to claim 1, characterized in that: The size of the ventilation opening is 30 cm × 30 cm, and the gauze is 200 meshes.

4. A device for rearing adults of Thrips leucophylla according to claim 1, characterized in that: The acrylic partition is 0.5 cm thick, has a size of 41 cm × 33 cm, and is provided with 36 (6 × 6) holes with a diameter of 2 cm.

5. The device for rearing adults of Thrips leucophylla according to claim 1, characterized in that: The upper chamber is 18 cm high, and the lower chamber is 24 cm high.

6. The device for raising adults of Thrips leucocephalus according to claim 1, characterized in that: A magnet sheet with a diameter of 1 cm is embedded in each corner of the box body and the box door, so that the box body and the box door are connected by magnetic attraction.

7. The device for rearing adults of Thrips leucophylla according to claim 1, characterized in that: The operating window on the box door has a size of 15 cm × 10 cm, and a 0.5 cn magnet is embedded in the window cover and the window, so that the window cover and the box door window are connected by magnetic attraction. The window cover has an opening of 13 cm × 8 cm and is covered with a 200-mesh gauze.

8. A method for indoor breeding of Thrips leucophylla, characterized in that: The indoor breeding device for Thrips leucophylla described in claims 1 to 7 is used, and the specific steps are as follows: (1) Transfer the adult thrips into the insect box and place the whole lentil pods on the upper layer of the insect box and lay them flat on the partition for the adult thrips to feed and lay eggs; (2) After 1-2 days (before the eggs hatch), insert the CO2 gun into the operating window to blow the bean thrips off the pods and drop them into the box. Take out the pods that have been feeding on the eggs and put in fresh pods. (3) Place the bean pods after feeding and laying eggs in a glass insect jar, wait for the eggs to hatch into nymphs, and continue to raise them; (4) After the adults emerge from their pupae, open the glass insect jar and place it directly into the lower layer of the insect box. Wait for the adults to gather on the fresh bean pods on the upper layer and continue to feed and lay eggs.

9. The device for rearing adults of Thrips leucophylla according to claim 8, characterized in that: In each of the boxes, 10-15 lentil pods are placed, which can hold 1000-4000 adult thrips.

10. The device for rearing adults of Thrips leucophylla according to claim 8, characterized in that: In the glass insect jar, 8-10 wrinkled napkins are placed at the bottom of the insect jar to provide a pupation environment for the thrips.

11. The device for rearing adults of Thrips leucophylla according to claim 8, characterized in that: Five bean pods after feeding and laying eggs are placed in the glass insect jar to ensure the humidity in the insect jar.

12. The device for rearing adults of Thrips leucophylla according to claim 8, characterized in that: The environmental conditions of the breeding method are as follows: temperature 25 ± 5 °C, relative humidity 60%-70%, and light intensity 16L:8L.

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