Application of prohexadione calcium in relieving plant salt stress
Through the spraying and application of calcium cycloplasmic acid, the problem of growth inhibition of rice under salt stress was solved, the salt tolerance and photosynthesis of rice were significantly improved, the water utilization was improved, and the effective relief of salt stress was achieved, and the promotion of The increase in rice yield.
Patent Information
- Application Number
- CN202411350247.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-26
- Publication Date
- 2025-01-10
AI Technical Summary
Soil salinization has a serious impact on rice production. The existing technology lacks understanding of the mechanism of action of calcium cycloplasmic acid under salt stress conditions, which affects the salt tolerance and yield of rice.
By spraying the leaves or applying calcium cyclostatic acid at the roots, the influence of its growth on rice at different concentrations was studied, and it was determined that the concentration of 60mgL-1~120mgL-1 can reduce plant height, increase leaf area and root crown ratio, and enhance photosynthetic Rate, transpiration rate and porosity conductivity improve moisture conditions and alleviate the toxic effects of salt stress on rice.
Calcium cycloplasmic acid significantly improves the growth performance of rice under salt stress, enhances salt tolerance, improves photosynthesis and water utilization efficiency, alleviates the toxic effects of salt stress on rice, and provides improved rice yield and salt alkali resistance. An effective way to competence.
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Figure CN119278944A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the field of biotechnology, and particularly relates to application of prohexadione-calcium in alleviating plant salt stress. Background Art
[0002] Soil salinization is a global problem that restricts agricultural development. Rice is the staple food of my country and the world. Soil salinization is one of the most serious abiotic stresses that endanger rice production and the most important environmental factor that inhibits the normal growth and development of rice. Research on the mechanism of rice salt resistance has become a hot topic in recent years. As a plant growth regulator, calcium cyclohexadione has been proven to play a positive role in alleviating oxidative damage caused by a variety of abiotic stresses. However, little is known about the mechanism of the effect of calcium cyclohexadione on rice under salt stress. In-depth exploration of the mechanism of calcium cyclohexadione regulating salt tolerance of sea rice under salt stress is of great significance to improving my country's rice yield level and salt-alkali tolerance and ensuring my country's food security. Summary of the invention
[0003] In view of the current situation of salt stress, the purpose of the present invention is to provide an application of prohexadione calcium in alleviating plant salt stress. The present invention experimentally studies the effect of spraying prohexadione calcium (Pro-CA) on the growth and resistance physiology of rice seedlings under salt stress, studies the alleviating effect of Pro-Ca on rice seedlings under salt stress conditions, and determines the concentration of Pro-Ca that is beneficial to rice salt tolerance.
[0004] In order to achieve the above-mentioned purpose, the present invention adopts the following technical solutions: The invention provides application of prohexadione-calcium in alleviating plant salt stress.
[0005] Furthermore, during the growth of plants, prohexadione-calcium is sprayed on the leaves and / or applied to the roots, so that crops can grow normally in a salt environment.
[0006] Furthermore, the use concentration of prohexadione calcium is 60 mg / L -1 ~120mgL -1 .
[0007] Furthermore, the prohexadione-calcium can reduce rice plant height, increase rice leaf area, increase dry matter mass and increase root-to-shoot ratio, thereby alleviating the inhibitory effect of salt stress on plant growth.
[0008] Furthermore, high concentration of prohexadione-calcium is most beneficial to increasing the dry matter, root-to-shoot ratio and leaf area of rice under low salt stress.
[0009] Furthermore, the prohexadione-calcium can increase the photosynthetic rate, transpiration rate and stomatal conductance of rice under salt stress, and the photosynthetic rate, transpiration rate and stomatal conductance all increase with the increase of the prohexadione-calcium concentration.
[0010] Furthermore, high concentration of prohexadione-calcium used in low-salt stress is most beneficial to increase the photosynthetic rate, transpiration rate and stomatal conductance of rice.
[0011] Furthermore, the prohexadione-calcium can increase the relative water content of leaves, leaf water potential and root water conductivity of plants under salt stress, and all of these increase with the increase of the prohexadione-calcium concentration.
[0012] Furthermore, the concentration of prohexadione calcium reaches 60 mg / L -1 It can effectively alleviate the impact of salt stress on plant leaf water potential.
[0013] Furthermore, the plant is rice.
[0014] Compared with the prior art, the present invention has the following beneficial effects: 1. The present invention proves through experiments that prohexadione-calcium can alleviate the inhibitory effect of salt stress on rice growth by reducing rice plant height, increasing rice leaf area, dry matter mass and increasing root-to-shoot ratio. High concentration of prohexadione-calcium is most beneficial to the dry matter, root-to-shoot ratio and leaf area of rice under low salt stress.
[0015] 2. Salt stress affects the photosynthesis of rice. Calcium cyclohexane can increase the photosynthesis of rice under salt stress, and the photosynthetic rate, transpiration rate and stomatal conductance all increase with the increase of Calcium cyclohexane concentration. The combination of high concentration of Calcium cyclohexane and low salt stress treatment is most conducive to the photosynthesis of rice.
[0016] 3. The relative water content of rice leaves, root hydraulic conductivity and leaf water potential were all negatively affected by salt stress, and increased with the increase of prohexadione-calcium concentration. High concentration of prohexadione-calcium was most beneficial to the relative water content of rice leaves and root hydraulic conductivity under low salt stress, and was most beneficial to the leaf water potential of rice under high salt stress.
[0017] 4. Under salt stress, calcium cyclamate improves the water status of plants (leaf water potential and root hydraulic conductivity), thereby increasing rice photosynthesis and promoting dry matter accumulation, thereby alleviating the toxic effects of salt stress on rice.
[0018] The present invention proves the effect of prohexadione calcium on alleviating rice salt stress through experiments, provides a basis for the application of Pro-CA in rice production, and has important theoretical and practical significance. BRIEF DESCRIPTION OF THE DRAWINGS
[0019] Figure 1 is the effect of different salt concentrations on the down-regulation of calcium cyclopentadienate on rice plant height; Figure 2 is the effect of different salt concentrations on the accumulation of rice dry matter by down-regulating calcium cyclopentadienate; Figure 3 Effects of downregulation of calcium cyclopentadienate on root-to-shoot ratio and leaf area of rice under different salt concentrations; Among them, different capital letters in the figure represent significant differences at the 0.05 level among CK, P1 and P2 treatments. Different lowercase letters represent significant differences at the 0.05 level within the group. DETAILED DESCRIPTION
[0020] The technical solution of the present invention is described in detail below with reference to the accompanying drawings and embodiments. Example
[0021] 1. Experimental Methods The experiment was conducted in the experimental greenhouse of Weifang Institute of Science and Technology in Shouguang City, Weifang City, Shandong Province in 2023. There are three factors in this experiment: prohexadione calcium, salt stress and rice varieties. For prohexadione calcium, two concentration levels were set, namely low concentration Pro-ca1 (P1=60mgL -1 ) and high concentration Pro-ca2 (P2 = 120 mg L -1 ), and the treatment without the application of prohexadione-calcium was used as the control treatment (CK). For salt stress, there were three salt stress treatments: 0, 50mmol / L and 100mmol / L NaCl (i.e., S0, S1 and S2). Two rice varieties were selected: Fuyou No. 19 (F) and Dachangjing (D). There were a total of 18 treatments, and each treatment was repeated 4 times. The detailed experimental factor treatment table is shown in Table 1.
[0022] Table 1 Experimental factor level table
[0023] 2. Measurement indicators 1. Plant height: The height of a rice plant is measured from the natural height from the base of the plant to the growth point of the main stem.
[0024] 2. Dry matter: Take rice seedlings of each treatment, weigh the leaves and roots respectively, put them in a 105°C oven for 30 min to kill green, and finally dry them in a 75°C oven to a constant weight and weigh them.
[0025] 3. Leaf area: The leaf area of plants was measured using a leaf area meter (LICOR 3100).
[0026] 4. Root-shoot ratio: root dry matter / aboveground dry matter.
[0027] 5. Determination of relative water content of leaves: Referring to the method of Baars, take plant leaves and quickly weigh the fresh weight (Wf). Immerse the plant material in distilled water and place it in a 4 ℃ refrigerator in the dark for 24 hours until constant weight, take the material out of the water, quickly absorb the surface water of the material with absorbent paper, and weigh its saturated fresh weight (Wfs). Put the above materials into an envelope, put it in an oven, and fix it at 105 ℃ for 30 minutes, then adjust the temperature to 80 ℃ until constant weight (Wd), and calculate the relative water content according to the formula: .
[0028] 6. Determination of leaf water potential: The water potential of the top, well-grown and fully expanded leaves of rice was measured by the dew point method using a dew point microvoltmeter (HR-33 T, Wescor, Logan, UT, USA).
[0029] 7. Determination of root hydraulic conductivity: Root hydraulic conductivity was determined using the pressure chamber method. Referring to the method of Gal et al (2020), the rice potted plants were pressurized in a pressure chamber and observed with a magnifying glass until juice flowed out of the cut surface (pressurized to about 0.1 MPa). The plants were allowed to adapt to this pressure for 2 min; after 2 min, the remaining exudate was removed and the timer was started for 3 min. The exudate was transferred to a centrifuge tube using a pipette; after collecting at 0.1 MPa pressure for 3 min, the pressure was increased to 0.2 MPa. Repeat the above steps, adding 0.1 MPa pressure each time and collecting 4 more centrifuge tubes. After measuring for 30 min, move the dial to the right to release the pressure; centrifuge the liquid in the 5 EP tubes, and then use a pipette to measure the volume V of each tube, and calculate the water flux Jv=V / 120 s. The total hydraulic conductivity of the root system is the slope of the relationship curve between the water flux (Jv) and the pressure difference, that is, the total hydraulic conductivity of the root system Lp=Jv / ΔP.
[0030] 3. Experimental Results 3.1 Effects of different salt concentrations on the growth of rice seedling height
[0031] Depend on Figure 1It can be seen that both salt concentration and prohexadione calcium concentration affect the plant height of the two rice plants. At low and high salt concentrations, the rice plant height decreased with the increase of the amount of prohexadione calcium applied. At high salt concentration (100mmol / L), there was a significant difference between the P2 treatment and the CK treatment for the D variety of rice, and P2 significantly decreased by 46.34% relative to CK. At low and high concentrations of prohexadione calcium, the rice plant height decreased with the increase of salt concentration. At high prohexadione calcium concentration, the plant height of F and D varieties of rice was significantly reduced by 23.72% and 43.79% under high salt concentration treatment compared with the treatment without salt stress (0mmol / L).
[0032] 3.2 Effects of calcium cyclopentadienate reduction at different salt concentrations on rice dry matter, leaf area, and root-shoot ratio
[0033] Depend on Figure 2 It can be seen that under the same prohexadione calcium concentration treatment, the dry matter accumulation of both rice varieties decreased with the increase of salt concentration. When the prohexadione calcium treatment was ignored, the dry matter accumulation of F and D varieties of rice under high salt concentration treatment was significantly reduced by 38.31% and 45.10% on average compared with the no salt stress treatment. Under the same salt concentration treatment, the dry matter accumulation of both rice varieties increased with the increase of prohexadione calcium concentration. Under high salt concentration treatment, the dry matter accumulation of F rice under P2 treatment increased significantly by 61.66% and 81.56% compared with P1 and CK treatments, respectively.
[0034] Figure 3 The effects of different salt concentrations and prohexadione-calcium treatments on the root-to-shoot ratio and leaf area of rice were shown in Figure 2. Figure 3 It can be seen that for the root-shoot ratio of rice, under different treatments of calcium cyclohexane, high salt stress reduced the root-shoot ratio of rice, but under the treatment of high concentration calcium cyclohexane P2, the root-shoot ratio of low salt stress treatment increased compared with the no salt stress treatment; for F variety rice, under salt stress treatment, the root-shoot ratio increased with the increase of calcium cyclohexane concentration, among which P2 increased significantly by 53.15% compared with CK under low salt stress treatment. For D variety rice, the root-shoot ratio also increased with the increase of calcium cyclohexane concentration, among which P2 increased significantly by 42.59% compared with CK under low salt stress treatment. It shows that under low salt stress conditions, the addition of high concentration calcium cyclohexane is more conducive to increasing the root-shoot ratio of rice.
[0035] Regarding rice leaf area, under the same prohexadione-calcium treatment, salt stress significantly affected the growth of rice leaf area. The leaf area of both rice varieties increased with the increase of prohexadione-calcium concentration. When treated with low salt concentration, the leaf area of F variety rice treated with P1 and P2 increased significantly by 25.74% and 22.79% compared with CK; and the leaf area of D variety rice increased significantly by 20.28% and 37.06% compared with CK. This shows that under low salt stress conditions, the effect of prohexadione-calcium on rice leaf area is more significant.
[0036] Therefore, experiments have shown that calcium cyclohexane can reduce rice plant height, increase rice leaf area, dry matter and increase root-shoot ratio, thereby alleviating the inhibitory effect of salt stress on rice growth. High concentrations of calcium cyclohexane are most beneficial to the dry matter, root-shoot ratio and leaf area of rice under low salt stress.
[0037] 3.3 Effects of different calcium cyclamate on photosynthetic rate, transpiration rate and stomatal conductance
[0038] Table 2 shows the effects of calcium cyclohexane on the photosynthetic rate, transpiration rate and stomatal conductance of rice under different salt concentrations. It can be seen from Table 2 that under the same calcium cyclohexane treatment, salt stress treatment reduced the photosynthetic rate, transpiration rate and stomatal conductance of both F and D rice compared with no salt stress.
[0039] As for the photosynthetic rate Pn, under salt stress, the Pn of both rice varieties increased with the increase of procyclohexane calcium. Under low salt stress, the P2 treatment significantly increased the photosynthetic rates of F and D rice by 53.80% and 69.79% compared with the CK treatment, respectively, while the P1 treatment significantly increased the photosynthetic rate of F rice by 50% compared with the CK treatment. Under high salt stress, the P2 treatment significantly increased the photosynthetic rates of F and D rice by 55.86% and 118.39% compared with the CK treatment, respectively, while the P1 treatment significantly increased the photosynthetic rate of F rice by 45.46% compared with the CK treatment.
[0040] For transpiration rate Tr, under the two salt stress treatments, Tr of both rice varieties increased with the increase of prohexadione-calcium and reached the maximum value at P2. The P2 treatment of F and D rice increased by 10.82% and 7.89% respectively compared with the CK treatment.
[0041] For stomatal conductance gs, under the two salt stress treatments, both rice varieties increased with the increase of prohexadione-calcium and reached the maximum value at P2. The P2 treatment of F and D rice increased by 50.67% and 59.52% respectively compared with the CK treatment. In summary, salt stress affects the photosynthesis of rice. Prohexadione-calcium can increase the photosynthesis of rice under salt stress, and the photosynthetic rate, transpiration rate and stomatal conductance all increase with the increase of prohexadione-calcium concentration. The combination of high concentration of prohexadione-calcium and low salt stress treatment is most beneficial to the photosynthesis of rice.
[0042] Table 2 Effects of calcium cyclopentadienyl sulfate on photosynthetic rate, transpiration rate and stomatal conductance at different salt concentrations
[0043] 3.4 Effects of calcium cyclohexadione on relative water content, root hydraulic conductivity, and leaf water potential at different salt concentrations
[0044] The relative water content of leaves is an important physiological index used to evaluate the water status and water retention capacity of plant leaves. The root hydraulic conductivity is an index indicating the ability of the root system to transport and conduct water, which plays a decisive role in the growth of rice seedlings. The leaf water potential is the most sensitive physiological index to measure the water deficit state in plants among the water potential of various tissues and organs of plants. It represents the ability of plants to absorb water from the soil or adjacent cells to ensure that the plants maintain normal physiological activities. As shown in Table 3, the relative water content of rice leaves, the root hydraulic conductivity and the leaf water potential all decreased with the increase of salt concentration. Prohexadione-calcium can increase the relative water content of leaves, the root hydraulic conductivity and the leaf water potential under salt stress, and all of them increased with the increase of the concentration of prohexadione-calcium.
[0045] Regarding the relative water content of leaves, under low salt stress, the relative water content of F rice leaves in P1 and P2 treatments increased significantly by 9.55% and 19.51% compared with that in CK treatment, respectively; the relative water content of D rice leaves increased by 6.17% and 9.70% compared with that in CK treatment, respectively. Under high salt concentration treatment, the relative water content of F rice leaves in P1 and P2 treatments increased by 4.20% and 17.62% compared with that in CK treatment, respectively; the relative water content of D rice leaves increased by 3.47% and 5.13% compared with that in CK treatment, respectively.
[0046] For root hydraulic conductivity, under low salt stress, the root hydraulic conductivity of F rice in P1 and P2 treatments increased significantly by 94.73% and 121.1% compared with CK treatment, respectively; the root hydraulic conductivity of D rice increased by 40% and 56% compared with CK treatment, respectively. Under high salt concentration treatment, the root hydraulic conductivity of F rice in P1 and P2 treatments increased by 37.5% and 56.25% compared with CK treatment, respectively; the root hydraulic conductivity of D rice increased by 27.3% and 50% compared with CK treatment, respectively.
[0047] For leaf water potential, under high and low salt stress, the leaf water potential of F rice increased significantly by 38% and 31.25% in P2 treatment compared with that in CK treatment, and by 2% and 8.3% in P1 treatment compared with that in CK treatment, respectively; the leaf water potential of D rice increased by 8.7%, 1.7%, 34.78% and 15.25% in P1 and P2 treatments compared with that in CK treatment, respectively.
[0048] In summary, prohexadione-calcium can increase the relative water content of rice leaves, root hydraulic conductivity and leaf water potential under salt stress. High concentration of prohexadione-calcium is most beneficial to the relative water content of leaves and root hydraulic conductivity under low salt stress, and is most beneficial to leaf water potential under high salt stress.
[0049] Table 3 Effects of calcium cyclopentadienate on leaf relative water content, root hydraulic conductivity and leaf water potential at different salt concentrations
[0050] 3.5 Relative analysis between photosynthetic rate, transpiration rate, stomatal conductance, relative water content, leaf water potential and root hydraulic conductivity As shown in Table 4, there are significant or extremely significant positive correlations between photosynthetic rate, transpiration rate, stomatal conductance, relative water content, leaf water potential and root hydraulic conductivity. There are extremely significant positive correlations between rice leaf water potential and root hydraulic conductivity and photosynthetic rate, transpiration rate and stomatal conductance, respectively. This shows that procyclohexadione-calcium improves rice photosynthesis by improving leaf water potential and root hydraulic conductivity under salt stress, thereby promoting rice dry matter accumulation and alleviating the toxic effects of salt stress on rice.
[0051] Table 4 Relative relationship among Pn, Tr, gs, relative water content, leaf water potential and root hydraulic conductivity
[0052] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for a person skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to replace some of the technical features therein by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions claimed to be protected by the present invention.
Claims
1. Application of calcium cyclohexadione in alleviating plant salt stress.
2. The use of prohexadione-calcium in alleviating plant salt stress according to claim 1, characterized in that: During the plant growth process, foliar spraying of prohexadione-calcium and / or application of prohexadione-calcium to the roots enables crops to grow normally in a salt environment.
3. The use of prohexadione-calcium in alleviating plant salt stress according to claim 1, characterized in that: The concentration of prohexadione calcium is 60 mg / L -1 ~120mgL -1 .
4. The use of prohexadione-calcium in alleviating plant salt stress according to claim 1, characterized in that: The prohexadione-calcium can reduce the plant height of rice, increase the leaf area of rice, increase the dry matter mass and increase the root-crown ratio, thereby alleviating the inhibitory effect of salt stress on plant growth.
5. The use of prohexadione-calcium in alleviating plant salt stress according to claim 4, characterized in that: High concentration of prohexadione-calcium is most beneficial to increasing the dry matter, root-to-shoot ratio and leaf area of rice under low salt stress.
6. The use of prohexadione-calcium in alleviating plant salt stress according to claim 1, characterized in that: The prohexadione-calcium can increase the photosynthetic rate, transpiration rate and stomatal conductance of rice under salt stress. The photosynthetic rate, transpiration rate and stomatal conductance all increased with the increase of cyclohexadione-calcium concentration.
7. The use of prohexadione-calcium in alleviating plant salt stress according to claim 6, characterized in that: High concentration of prohexadione-calcium used in low-salt stress is most beneficial to increase the photosynthetic rate, transpiration rate and stomatal conductance of rice.
8. The use of prohexadione-calcium in alleviating plant salt stress according to claim 1, characterized in that: The prohexadione-calcium can increase the relative water content of leaves, leaf water potential and root water conductivity of plants under salt stress, and all of the water contents increase with the increase of the concentration of prohexadione-calcium.
9. The use of prohexadione-calcium in alleviating plant salt stress according to claim 8, characterized in that: The concentration of prohexadione calcium reaches 60 mg / L -1 It can effectively alleviate the impact of salt stress on plant leaf water potential.
10. The use of prohexadione-calcium in alleviating plant salt stress according to claim 1, characterized in that: The plant is rice.