Composition for dispelling effects of alcohol and protecting liver as well as preparation method, application and product thereof

Abstract

The invention provides a composition for dispelling effects of alcohol and protecting liver as well as a preparation method, application and a product thereof, and relates to the technical field of food. The alcohol effect dispelling and liver protecting composition comprises the following components: 50-200 parts of ampelopsis grossedentata leaf instant powder, 1-5 parts of a turmeric extract, 2-8 parts of corn oligopeptide powder, 2-8 parts of hovenia dulcis thunb powder, 1.4-7 parts of globe artichoke powder and 0.2-1 part of a licorice extract. Preparation of the turmeric extract: mixing turmeric with water, adjusting the pH value to 8.0-9.5, and performing ultrasonic treatment for the first time; carrying out reflux extraction at 75-90 DEG C for 4-7 hours, and carrying out secondary ultrasonic treatment; preparing a licorice extract: mixing licorice powder with water at 45-55 DEG C, and extracting for the first time; raising the temperature to 65-75 DEG C, and carrying out secondary extraction; and raising the temperature to 80-85 DEG C, and carrying out third extraction. The composition disclosed by the invention can quickly and efficiently dispel the effects of alcohol, has the effect of protecting the liver, and is applied to a product for dispelling the effects of alcohol, and an oral liquid product is high in stability.

Description

Technical Field

[0001] The invention belongs to the field of food technology, and specifically relates to a hangover-relief and liver-protection composition and a preparation method, application and product thereof. Background Art

[0002] Excessive drinking can cause serious damage to the liver, and long-term heavy drinking can lead to chronic alcohol poisoning, fatty liver, hepatitis, and cirrhosis. Alcohol is oxidized by alcohol dehydrogenase (ADH) in the liver to produce acetaldehyde, which is oxidized by acetaldehyde dehydrogenase (ALDH) to acetic acid, which is further metabolized into carbon dioxide and water. However, many people lack acetaldehyde dehydrogenase in their bodies, so they cannot break down acetaldehyde in time. Acetaldehyde enters the blood and causes damage to important organs and functions. In order to reduce the occurrence of this problem, researchers focus on developing a product to sober up and protect the liver to protect the liver health of drinkers.

[0003] The chemical components of licorice are mainly flavonoids, triterpenes, and estrogen-like substances. Glycyrrhizic acid is the main active substance in licorice, which has a protective effect on the liver and also has an anti-lipid peroxidation effect, protecting carbon tetrachloride from acute liver damage and alcoholic liver damage. Turmeric contains curcumin, which protects the liver from detoxification, reduces the damage of toxins in the liver to the liver, and promotes liver metabolism.

[0004] The leaves of Agave glabra can activate the alcohol-detoxifying enzyme in the liver, thus having a significant alcohol-detoxifying effect. The dihydromyricetin in the leaves of Agave glabra can reduce the ethanol concentration in the blood, which helps to sober up quickly. Corn oligopeptide powder helps to reduce the ethanol and acetaldehyde content in the blood, and has the activity of sobering up; while Hovenia dulcis has the effects of clearing heat and promoting diuresis, quenching thirst and relieving restlessness, and detoxifying alcohol, and has a preventive and therapeutic effect on acute alcohol poisoning. Artichoke is a perennial herbaceous plant of the genus Cynara in the Asteraceae family, mainly containing functional ingredients such as flavonoids, sesquiterpene lactones, phenols, and lignans; studies have shown that artichoke extract has the effect of sobering up and detoxifying, can repair damaged liver cells, and has a certain therapeutic effect on liver damage caused by alcohol.

[0005] Chinese invention patent CN114451562A discloses a composition, a drink and a preparation method thereof, wherein the composition comprises the following components by weight: 1-3 parts of corn oligopeptide powder, 0.01-1 parts of Tremella polysaccharide, 0.5-2.5 parts of Hovenia dulcis extract, 0.1-1 parts of Poria extract, 0.1-1 parts of Pueraria extract, 0.01-1 parts of turmeric, 0.01-1 parts of artichoke extract, 1-3 parts of honey, 0.1-1 parts of taurine, 0.01-1 parts of guarana extract, 0.01-1 parts of ginger, 0.01-1 parts of gamma-aminobutyric acid, 3-7 parts of oligofructose and 0.01-1 parts of lactic acid bacteria. The composition enhances the liver's detoxification function, promotes the metabolism of alcohol, accelerates the rate of excretion from the body, forms mucosal protection in the stomach to reduce the absorption of alcohol, and thus achieves the effect of sobering up and protecting the liver.

[0006] Chinese invention patent CN117502650A discloses a composition and its preparation method and application, wherein the composition is composed of kudzu root extract, rattan tea extract, artichoke extract, Hovenia dulcis extract, Poria extract, Hericium erinaceus extract and sugar alcohol. The invention prepares a granular composition by compounding erythritol or xylitol with six raw materials and further using a spray granulation method, which has good instant dissolution effect, less auxiliary materials, high bioavailability, low cost, and the extract is not easy to agglomerate and does not stick to the wall. It has the functions of accelerating alcohol metabolism, assisting protection against chemical liver damage, and nourishing the stomach.

[0007] Existing hangover products have relatively single functions. Most of them can only relieve the state of drunkenness. Moreover, their ingredients are single in function, and no further research has been conducted on the synergistic effect of the components in protecting the liver. Therefore, it is urgent to study a new hangover and liver protection product, conduct further in-depth research on the selection and proportion of components, make full use of the synergistic effect between the components, significantly reduce the sobering time, reduce the alcohol concentration, and increase NAD + content, reducing the content of MDA, AST and ALT, and the prepared product has high stability. Summary of the invention

[0008] In view of the problems existing in the prior art, the present invention provides a hangover-relieving and liver-protecting composition and its preparation method, application and product. The present invention prepares the composition by using the following components with specific ingredients and proportions: Agave ulmoides leaf instant powder, turmeric extract, corn oligopeptide powder, Hovenia dulcis powder, artichoke powder and licorice extract; wherein the turmeric extract is prepared by two ultrasonic extraction methods, and the licorice extract is prepared by a gradient temperature extraction method, so that the obtained composition can achieve the effects of rapid hangover relief and efficient liver protection; the present invention applies the hangover-relieving and liver-protecting composition to the preparation of hangover-relieving and liver-protecting products, and obtains a highly stable hangover-relieving and liver-protecting oral liquid by high-pressure homogenization with the components xanthan gum, gum arabic and sorbitol.

[0009] To achieve the above purpose, the technical solution adopted by the present invention is as follows: First, the present invention provides a hangover-relief and liver-protecting composition, which is composed of the following ingredients by weight: 50-200 parts of Ampelopsis grossedentata leaf instant powder, 1-5 parts of turmeric extract, 2-8 parts of corn oligopeptide powder, 2-8 parts of Hovenia dulcis powder, 1.4-7 parts of artichoke powder and 0.2-1 parts of liquorice extract; The preparation method of turmeric extract comprises the following steps: mixing turmeric with water, adjusting the pH to 8.0-9.5, performing a first ultrasonic treatment; then heating and refluxing extraction at 75-90° C. for 4-7 hours; and finally performing a second ultrasonic treatment and filtering to obtain the turmeric extract; The preparation method of licorice extract comprises the steps of: mixing licorice powder with hot water at 45-55 DEG C for the first extraction; then heating to 65-75 DEG C for the second extraction; heating to 80-85 DEG C for the third extraction, and filtering to obtain the licorice extract.

[0010] Preferably, the alcohol sobering and liver protecting composition is composed of the following ingredients by weight: 100-150 parts of Ampelopsis grossedentata leaf instant powder, 2-4 parts of turmeric, 4-6 parts of corn oligopeptide powder, 4-7 parts of Hovenia dulcis powder, 2.5-5.5 parts of artichoke powder and 0.5-0.8 parts of liquorice.

[0011] Further preferably, the alcohol sobering and liver protecting composition is composed of the following ingredients, by weight: 130 parts of Ampelopsis grossedentata leaf instant powder, 3 parts of turmeric, 5 parts of corn oligopeptide powder, 5 parts of Hovenia dulcis powder, 4 parts of artichoke powder and 0.7 parts of liquorice.

[0012] Preferably, the method for preparing the turmeric extract comprises the steps of: (1) crushing turmeric to a particle size of 40-80 mesh, mixing with water at a solid-liquid ratio of 1:15-25 g / mL, adjusting the pH to 8.5-9.0, and performing a first ultrasonic treatment for 20-40 minutes to obtain a first ultrasonic treatment mixture; (2) subjecting the first ultrasonic treatment mixture of step (1) to reflux extraction at 80-90° C. for 5-6 hours to obtain a reflux extraction mixture; (3) subjecting the reflux extraction mixture of step (2) to a second ultrasonic treatment for 30-50 min, and filtering to obtain a turmeric extract; (4) Concentrating and drying the turmeric extract obtained in step (3) to obtain a turmeric extract.

[0013] Further preferably, the method for preparing the turmeric extract comprises the steps of: (1) Grinding turmeric to a particle size of 60 mesh, mixing with water at a solid-liquid ratio of 1:20 g / mL, adjusting the pH to 8.8, and performing a first ultrasonic treatment for 30 min to obtain a first ultrasonic treatment mixture; (2) subjecting the first ultrasonic treatment mixture of step (1) to reflux extraction at 85° C. for 5 h to obtain a reflux extraction mixture; (3) subjecting the reflux extraction mixture of step (2) to a second ultrasonic treatment for 40 min, and filtering to obtain a turmeric extract; (4) Concentrating and drying the turmeric extract obtained in step (3) to obtain a turmeric extract.

[0014] Preferably, the preparation method of the licorice extract comprises the steps of: S1, mixing licorice powder with a particle size of 40-80 mesh with hot water at 45-50° C. at a solid-to-liquid ratio of 1:15-25 g / mL, stirring for a first extraction for 0.5-2h, and obtaining a first extraction mixture; S2, heating the first extraction mixture of step S1 to 65-70° C., stirring and performing a second extraction for 2-5 hours to obtain a second extraction mixture; S3, heating the second extraction mixture of step S2 to 80-85°C, stirring for a third extraction for 0.5-2h, and filtering to obtain a licorice extract; S4, concentrating and drying the liquorice extract obtained in step S3 to obtain a liquorice extract.

[0015] Further preferably, the preparation method of the licorice extract comprises the steps of: S1, licorice powder with a particle size of 60 mesh, mixed with 50°C hot water at a solid-liquid ratio of 1:20 g / mL, stirred for the first extraction for 1 hour, and obtained a first extraction mixture; S2, heating the first extraction mixture of step S1 to 70° C., stirring and performing a second extraction for 3 hours to obtain a second extraction mixture; S3, heating the second extraction mixture of step S2 to 85° C., stirring for a third extraction for 1 hour, and filtering to obtain a licorice extract; S4, concentrating and drying the liquorice extract obtained in step S3 to obtain a liquorice extract.

[0016] Then, the present invention provides a preparation method of the above-mentioned alcohol detoxification and liver protection composition, comprising the steps of: screening Ampelopsis grossularis leaf instant powder, Hovenia dulcis powder, and artichoke powder with a particle size of 80-150 meshes; mixing and stirring the Ampelopsis grossularis leaf instant powder, corn oligopeptide powder, and licorice extract to obtain a mixture 1; mixing and stirring the turmeric extract, Hovenia dulcis powder, and artichoke powder to obtain a mixture 2; and stirring the mixture 1 and the mixture 2 to obtain the alcohol detoxification and liver protection composition.

[0017] Further preferably, the preparation method of the alcohol-detoxifying and liver-protecting composition comprises the steps of: screening the instant powder of Ampelopsis grossularis leaves, Hovenia dulcis powder and artichoke powder with a particle size of 100 mesh; mixing and stirring the instant powder of Ampelopsis grossularis leaves, corn oligopeptide powder and licorice extract to obtain mixture 1; mixing and stirring the turmeric extract, Hovenia dulcis powder and artichoke powder to obtain mixture 2; and stirring mixture 1 and mixture 2 to obtain the alcohol-detoxifying and liver-protecting composition.

[0018] Furthermore, the present invention provides application of the alcohol sobering and liver protecting composition in food preparation.

[0019] Preferably, the food is a food that has the functions of rapidly decomposing alcohol, shortening hangover time, protecting the liver, and resisting oxidation.

[0020] Preferably, the food is not limited to liquid drinks, solid drinks, soft candies, and tablets.

[0021] Finally, the present invention provides an oral liquid for sobering up and protecting the liver, the ingredients of which include, by weight: 10-30 parts of the above-mentioned composition for sobering up and protecting the liver, 1-4 parts of xanthan gum, 0.3-1.5 parts of gum arabic, 4-7 parts of sorbitol and 300-500 parts of water; The preparation method of the alcohol-relieving and liver-protecting oral liquid comprises the following steps: ①, after mixing the alcohol-detoxifying and liver-protecting composition with xanthan gum and water, homogenize under high pressure of 2-5 MPa to obtain a homogenous mixture; ②. Mix the homogenized mixture of step ① with gum arabic and sorbitol, and homogenize under high pressure of 6-8 MPa to obtain an oral liquid for alcohol sobering up and protecting the liver.

[0022] Preferably, the oral liquid for hangover relief and liver protection comprises, by weight: 20 parts of the above-mentioned hangover relief and liver protection composition, 2 parts of xanthan gum, 1 part of gum arabic, 6 parts of sorbitol and 400 parts of water.

[0023] Preferably, in step ①, the pressure of the high-pressure homogenization is 3-4 MPa, and the homogenization is circulated 2-4 times.

[0024] Further preferably, in step ①, the pressure of the high-pressure homogenization is 4 MPa, and the homogenization is circulated 3 times.

[0025] Preferably, in step ②, the pressure of the high-pressure homogenization is 6-7 MPa, and the homogenization is circulated 2-4 times.

[0026] Further preferably, in step ②, the pressure of the high-pressure homogenization is 7 MPa, and the homogenization is circulated for 3 times.

[0027] Compared with the prior art, the present invention has the following beneficial effects: 1. The alcohol sobering and liver protecting composition of the present invention is prepared by synergizing the instant powder of Ampelopsis serrulata leaves, corn oligopeptide powder, licorice extract, turmeric extract, Hovenia dulcis powder and artichoke powder. Each component is indispensable, which can significantly reduce the alcohol concentration and increase the NAD + Content, the effect of reducing MDA, AST and ALT levels.

[0028] 2. In the hangover-relieving and liver-protecting composition of the present invention, turmeric is subjected to two ultrasound-assisted alkaline water extraction processes, and the obtained turmeric extract has a high content of active ingredients and has the functions of effectively inhibiting human inflammatory response, anti-oxidation, and scavenging free radicals; the turmeric extract interacts with the instant powder of Agave sphenanthera leaves, Hovenia dulcis powder, licorice extract and artichoke powder to improve the hangover-relieving effect, and has excellent liver-protecting and anti-oxidation effects.

[0029] 3. In the alcohol-detoxifying and liver-protecting composition of the present invention, the licorice extract obtained by treating licorice powder with a gradient temperature extraction process is rich in various active ingredients: flavonoids, glycyrrhizic acid, terpenoid compounds, etc.; each component can protect drunk people from acute liver damage and alcoholic liver damage, and has the function of improving liver protection and accelerating alcohol decomposition.

[0030] 4. The hangover-detoxifying and liver-protecting composition of the present invention is used to prepare foods that can quickly decompose alcohol, shorten hangover-detoxifying time, and protect the liver; it is compounded with xanthan gum, gum arabic, and sorbitol, and a high-pressure homogenization method is used to prepare an oral liquid, and the oral liquid has high stability. DETAILED DESCRIPTION

[0031] The following non-limiting examples can enable those skilled in the art to more fully understand the present invention, but do not limit the present invention in any way. The following content is merely an exemplary description of the scope of the present invention, and those skilled in the art can make various changes and modifications to the present invention based on the disclosed content, which should also fall within the scope of the present invention.

[0032] When the embodiments give numerical ranges, it should be understood that, unless otherwise specified in the present invention, both endpoints of each numerical range and any numerical value between the two endpoints can be selected. Unless otherwise defined, all technical and scientific terms used in the present invention have the same meanings as those generally understood by those of ordinary skill in the art to which the present invention belongs.

[0033] The present invention is further described below by way of specific examples. Unless otherwise specified, the various chemical reagents used in the examples of the present invention are obtained through conventional commercial channels.

[0034] In the following examples, the Ampelopsis grossedentata leaf instant powder was purchased from Shaanxi Jiuyuan Biotechnology Co., Ltd.; the corn oligopeptide powder was purchased from Zhejiang Haishi Biotechnology Co., Ltd.; the Hovenia dulcis powder was purchased from Yangling Ruifen Biotechnology Co., Ltd.; and the artichoke powder was purchased from Symrise, France. Products from different manufacturers have no significant effect on the effect.

[0035] Example 1-Example 3 A hangover-relieving and liver-protecting composition, comprising the components shown in Table 1 in parts by weight: Table 1

[0036] In Example 1 to Example 3, the preparation method of the turmeric extract is: (1) Grinding turmeric to a particle size of 60 mesh, mixing with water at a solid-liquid ratio of 1:20 g / mL, adjusting the pH of the water to 8.8 with sodium carbonate, and performing a first ultrasonic treatment for 30 minutes to obtain a first ultrasonic treatment mixture; (2) subjecting the first ultrasonic treatment mixture of step (1) to reflux extraction at 85° C. for 5 h to obtain a reflux extraction mixture; (3) subjecting the reflux extraction mixture of step (2) to a second ultrasonic treatment for 40 min, and filtering to obtain a turmeric extract; (4) Concentrating and drying the turmeric extract obtained in step (3) to obtain a turmeric extract.

[0037] In Examples 1 to 3, the preparation method of the licorice extract is: S1, licorice powder with a particle size of 60 mesh, mixed with 50°C hot water at a solid-liquid ratio of 1:20 g / mL, stirred for the first extraction for 1 hour, and obtained a first extraction mixture; S2, heating the first extraction mixture of step S1 to 70° C., stirring and performing a second extraction for 3 hours to obtain a second extraction mixture; S3, heating the second extraction mixture of step S2 to 85° C., stirring for a third extraction for 1 hour, and filtering to obtain a licorice extract; S4, concentrating and drying the liquorice extract obtained in step S3 to obtain a liquorice extract.

[0038] In Example 1 to Example 3, the preparation method of the alcohol-detoxifying and liver-protecting composition is as follows: the particle size of the instant powder of Agave ulmoides leaves, Hovenia dulcis powder and artichoke powder is 100 mesh; the instant powder of Agave ulmoides leaves, corn oligopeptide powder and licorice extract are mixed and stirred evenly to obtain mixture 1; the turmeric extract, Hovenia dulcis powder and artichoke powder are mixed and stirred evenly to obtain mixture 2; the mixture 1 and the mixture 2 are stirred evenly to obtain the alcohol-detoxifying and liver-protecting composition.

[0039] Example 4-Example 5 A hangover-relieving and liver-protecting composition, comprising the components shown in Table 2 in parts by weight: Table 2

[0040] In Example 4, the preparation method of the turmeric extract is: (1) Grinding turmeric to a particle size of 40 mesh, mixing with water at a solid-liquid ratio of 1:25 g / mL, adjusting the pH of the water to 9.0 with sodium carbonate, and performing a first ultrasonic treatment for 40 minutes to obtain a first ultrasonic treatment mixture; (2) subjecting the first ultrasonic treatment mixture of step (1) to reflux extraction at 80° C. for 6 h to obtain a reflux extraction mixture; (3) subjecting the reflux extraction mixture of step (2) to a second ultrasonic treatment for 50 min, and filtering to obtain a turmeric extract; (4) Concentrating and drying the turmeric extract obtained in step (3) to obtain a turmeric extract.

[0041] In Example 4, the preparation method of the licorice extract is: S1, licorice powder with a particle size of 40 mesh, mixed with 45°C hot water at a solid-liquid ratio of 1:25 g / mL, stirred for the first extraction for 2 hours, and obtained a first extraction mixture; S2, heating the first extraction mixture of step S1 to 65° C., stirring and performing a second extraction for 2 h to obtain a second extraction mixture; S3, heating the second extraction mixture of step S2 to 80° C., stirring for a third extraction for 2 h, and filtering to obtain a licorice extract; S4, concentrating and drying the liquorice extract obtained in step S3 to obtain a liquorice extract.

[0042] In Example 5, the preparation method of the turmeric extract is: (1) Grinding turmeric to a particle size of 80 mesh, mixing with water at a solid-liquid ratio of 1:15 g / mL, adjusting the pH of the water to 8.5 with sodium carbonate, and performing a first ultrasonic treatment for 20 minutes to obtain a first ultrasonic treatment mixture; (2) subjecting the first ultrasonic treatment mixture of step (1) to reflux extraction at 90° C. for 5 h to obtain a reflux extraction mixture; (3) subjecting the reflux extraction mixture of step (2) to a second ultrasonic treatment for 40 min, and filtering to obtain a turmeric extract; (4) Concentrating and drying the turmeric extract obtained in step (3) to obtain a turmeric extract.

[0043] In Example 5, the preparation method of the licorice extract is: S1, licorice powder with a particle size of 80 mesh, mixed with hot water at 55°C at a solid-liquid ratio of 1:15 g / mL, stirred for the first extraction for 0.5 h, to obtain a first extraction mixture; S2, heating the first extraction mixture of step S1 to 75° C., stirring and performing a second extraction for 5 hours to obtain a second extraction mixture; S3, heating the second extraction mixture of step S2 to 85°C, stirring for a third extraction for 0.5h, and filtering to obtain a licorice extract; S4, concentrating and drying the liquorice extract obtained in step S3 to obtain a liquorice extract.

[0044] The preparation method of the alcohol-relieving and liver-protecting composition described in Examples 4 and 5 is the same as that in Example 1.

[0045] Comparative Example 1 Different from Example 1, the following ingredients are not included: turmeric extract, Hovenia dulcis seed powder and artichoke powder.

[0046] The alcohol sobering and liver protecting composition is composed of the following ingredients by weight: 130 parts of Ampelopsis grossedentata leaf instant powder, 5 parts of corn oligopeptide powder and 0.7 parts of liquorice extract. The rest is the same as in Example 1.

[0047] Comparative Example 2 The difference from Example 1 is that the raw material components: Ampelopsis grossedentata leaf instant powder, corn oligopeptide powder and licorice extract are not contained.

[0048] The alcohol-relief and liver-protecting composition is composed of the following ingredients by weight: 3 parts of turmeric extract, 5 parts of Hovenia dulcis powder and 4 parts of artichoke powder. The rest is the same as in Example 1.

[0049] Comparative Example 3 The difference from Example 1 is that the liquorice extract is replaced by a turmeric extract. The rest is the same as Example 1.

[0050] The alcohol sobering and liver protecting composition is composed of the following ingredients by weight: 130 parts of Ampelopsis grossedentata leaf instant powder, 3.7 parts of turmeric extract, 5 parts of corn oligopeptide powder, 5 parts of Hovenia dulcis powder and 4 parts of artichoke powder.

[0051] Comparative Example 4 The difference from Example 1 is that the Ampelopsis grossedentata leaf instant powder is replaced with Pueraria root powder. The rest is the same as Example 1.

[0052] Comparative Example 5 The difference from Example 1 is that the weight ratio of each raw material component in the hangover-relieving and liver-protecting composition is different.

[0053] The alcohol-detoxifying and liver-protecting composition is composed of the following ingredients: 130 parts of Ampelopsis grossedentata leaf instant powder, 0.5 parts of turmeric extract, 1.7 parts of corn oligopeptide powder, 12 parts of Hovenia dulcis powder, 0.5 parts of artichoke powder and 3 parts of liquorice extract. The rest is the same as in Example 1.

[0054] Comparative Example 6 Different from Example 1, the preparation method of turmeric extract is different, pH adjustment and the first ultrasonic treatment are not performed, and the specific steps are as follows: (1) Grinding turmeric to a particle size of 60 mesh, mixing with water at a solid-liquid ratio of 1:20 g / mL, and reflux extraction at 85°C for 5 h to obtain a reflux extraction mixture; (2) subjecting the reflux extraction mixture of step (1) to ultrasonic treatment for 40 minutes, and filtering to obtain a turmeric extract; (3) Concentrating and drying the turmeric extract obtained in step (2) to obtain a turmeric extract.

[0055] The rest are the same as in Example 1.

[0056] Comparative Example 7 Different from Example 1, the preparation method of licorice extract is different, and the gradient temperature extraction step is not performed. The specific steps are: licorice powder with a particle size of 60 mesh is mixed with 85°C hot water at a solid-liquid ratio of 1:20 g / mL, and stirred for extraction for 5 hours to obtain a licorice extract; the licorice extract is concentrated and dried to obtain a licorice extract.

[0057] The rest are the same as in Example 1.

[0058] Application Example 1 The hangover-detoxifying and liver-protecting composition of Example 1 was used to prepare a hangover-detoxifying and liver-protecting oral liquid.

[0059] The hangover-relieving and liver-protecting oral liquid is composed of the following components in parts by weight: 20 parts of the hangover-relieving and liver-protecting composition of Example 1, 2 parts of xanthan gum, 1 part of gum arabic, 6 parts of sorbitol and 400 parts of water.

[0060] The preparation method of the alcohol-relieving and liver-protecting oral liquid is as follows: ① After the alcohol sobering and liver protecting composition of Example 1 is mixed with xanthan gum and water, high pressure homogenization is performed at 4 MPa, and the homogenization is circulated for 3 times to obtain a homogenous mixture; ②. Mix the homogenized mixture of step ① with gum arabic and sorbitol, homogenize under 7 MPa high pressure, and circulate the homogenization for 3 times to obtain an oral liquid for alcohol sobering up and protecting the liver.

[0061] Application Example 2 The difference from Application Example 1 is that the weight portions of the components of the alcohol-relieving and liver-protecting oral liquid are different.

[0062] The hangover-relieving and liver-protecting oral liquid is composed of the following components in parts by weight: 10 parts of the hangover-relieving and liver-protecting composition of Example 1, 1 part of xanthan gum, 0.3 parts of gum arabic, 4 parts of sorbitol and 300 parts of water.

[0063] The rest is the same as that of Application Example 1.

[0064] Application Example 3 The difference from Application Example 1 is that the weight portions of the components of the alcohol-relieving and liver-protecting oral liquid are different.

[0065] The hangover-relieving and liver-protecting oral liquid is composed of the following components in parts by weight: 30 parts of the hangover-relieving and liver-protecting composition of Example 1, 4 parts of xanthan gum, 1.5 parts of gum arabic, 7 parts of sorbitol and 500 parts of water.

[0066] The rest is the same as that of Application Example 1.

[0067] Application Comparative Example 1 Different from Application Example 1, xanthan gum is replaced by gum arabic.

[0068] The alcohol-detoxifying and liver-protecting oral liquid is composed of the following components in parts by weight: 20 parts of the alcohol-detoxifying and liver-protecting composition of Example 1, 3 parts of gum arabic, 6 parts of sorbitol and 400 parts of water.

[0069] The preparation method of the alcohol-relieving and liver-protecting oral liquid is as follows: ① The hangover-detoxifying and liver-protecting composition of Example 1 was mixed with 1 part of gum arabic and water, and then homogenized under high pressure at 4 MPa for 3 cycles to obtain a homogenous mixture; ②. Mix the homogenized mixture of step ① with 2 parts of gum arabic and 6 parts of sorbitol, homogenize under 7 MPa high pressure, and circulate the homogenization for 3 times to obtain an oral liquid for alcohol sobering up and protecting the liver.

[0070] Application Comparative Example 2 Different from Application Example 1, gum arabic and sorbitol as a whole are replaced by xanthan gum.

[0071] The alcohol-detoxifying and liver-protecting oral liquid is composed of the following components in parts by weight: 20 parts of the alcohol-detoxifying and liver-protecting composition of Example 1, 9 parts of xanthan gum and 400 parts of water.

[0072] The preparation method of the alcohol-relieving and liver-protecting oral liquid is as follows: ① After the alcohol sobering and liver protecting composition of Example 1 is mixed with xanthan gum and water, high pressure homogenization is performed at 4 MPa, and the homogenization is circulated for 3 times to obtain a homogenous mixture; ②. The homogenized mixture of step ① was subjected to high pressure homogenization at 7 MPa for 3 cycles to obtain an oral liquid for alcohol detoxification and liver protection.

[0073] Application Comparative Example 3 The difference from Application Example 1 is that sorbitol is replaced by erythritol.

[0074] The preparation method of the alcohol-relieving and liver-protecting oral liquid is as follows: ① After the alcohol sobering and liver protecting composition of Example 1 is mixed with xanthan gum and water, high pressure homogenization is performed at 4 MPa, and the homogenization is circulated for 3 times to obtain a homogenous mixture; ②. Mix the homogenized mixture of step ① with gum arabic and erythritol, homogenize under high pressure at 7 MPa, and circulate the homogenization for 3 times to obtain an oral liquid for alcohol sobering up and protecting the liver.

[0075] Experiment 1: Testing of the efficacy of alcohol detoxification and liver protection 1. Cell culture HepG2 hepatocytes were stored in DMEM medium supplemented with 10% fetal bovine serum, 100 U / mL penicillin, and 0.1 mg / mL streptomycin, and subcultured in an incubator at 37°C and 5% CO2 saturated humidity. The medium was changed every 2 days, and the cells were digested with 0.1% trypsin every 3-4 days and subcultured at a ratio of 1:3.

[0076] 2. The effect of the alcohol-detoxifying and liver-protecting composition on the levels of alcohol metabolic enzymes and oxidative stress in ethanol-treated hepatocytes HepG2.

[0077] Take HepG2 cells in the logarithmic growth phase, digest the cells with 0.1% trypsin solution, centrifuge, make a single cell suspension, and count. Adjust the cell concentration to 5×10 5 / mL, inoculated in a 6-well culture plate and set aside.

[0078] Experimental settings: normal group, model group, experimental group (alcoholism and liver protection compositions of the embodiment and comparative example): (1) Normal group: cultured in normal culture medium.

[0079] (2) Model group: 2% ethanol was added to the normal group for culture.

[0080] (3) Experimental group: On the basis of the model group, each alcohol-detoxifying and liver-protecting composition was added at a concentration of 4 mg / mL for culture.

[0081] All the above groups were placed in an incubator at 37°C and 5% CO2 saturated humidity for 48 hours. After 48 hours, the culture medium was removed, the cells were carefully washed twice with PBS buffer, RIPA lysis buffer was added, and after full lysis, the cells were centrifuged at 3000 r / min for 3-5 minutes, the supernatant was taken, and NAD + , MDA, AST, and ALT kits were used for determination.

[0082] Among them, since the MDA content can reflect the body's oxidative stress level, it can also reflect the degree of tissue peroxidation damage. In addition, AST and ALT are mainly present in liver cells. Under normal circumstances, due to the barrier function of the liver cell membrane, only a very small amount of AST and ALT can pass through the cell membrane into the blood. When liver cells are damaged, the permeability of the cell membrane increases. At this time, the amount of AST and ALT entering the blood increases significantly, and the degree of liver cell damage is proportional to the amount of AST and ALT permeable. Therefore, AST and ALT levels are often used as important markers to judge the degree of liver damage.

[0083] 3. Experimental results Table 3 shows the NAD concentrations of each group of experiments. + , MDA, AST, and ALT content results.

[0084] Table 3

[0085] In Table 3, each group was compared with the model group: △ P<0.05, △△ P<0.01, with significant difference; compared with the normal group: & P<0.05, && P<0.01; Comparative Example Group Compared with Example 1 Group: # P<0.05, ## P<0.01, there is a significant difference.

[0086] As shown in Table 3, compared with the normal group, after ethanol treatment of HepG2 cells, NAD + The content decreased significantly, indicating that the metabolism of ethanol in liver cells consumed a large amount of NAD + ; MDA content increased significantly, indicating that ethanol treatment caused a significant increase in the level of oxidative stress in hepatocytes. The β-oxidation process of fatty acids in hepatocytes is the main pathway of lipid metabolism, which requires NAD + As a coenzyme. When ethanol metabolism consumes a lot of NAD + After that, the β-oxidation process of fatty acids is blocked, and lipid peroxidation occurs, MDA content increases, liver cells are damaged, and AST and ALT levels increase significantly.

[0087] In the experimental group, after using the alcohol detoxification and liver protection composition of the embodiment of the present invention, the above indicators were improved to varying degrees. Specifically, it can be seen from Table 3 that the alcohol detoxification and liver protection composition of the present invention significantly increased NAD + The technical effect of significantly reducing the content of MDA, AST and ALT has a better liver protection effect than the control group, and there are statistical differences between the effects.

[0088] Test 2 Alcohol metabolism test 1. Determine the alcohol sobering ability of the alcohol sobering and liver protecting composition at an alcohol concentration of 1000 mg / 100 mL. Use alcohol detection kit: EnzyChrom TM Ethanol Assay Kit (Cat. No. ECET-100) was used for detection.

[0089] HepG2 cells were cultured in groups: (1) Model group: Add 1000 mg / 100 mL of alcohol to the normal group for culture.

[0090] (2) Experimental group: On the basis of the model group, each alcohol-detoxifying and liver-protecting composition was added at a concentration of 4 mg / mL for culture.

[0091] After 3 hours of incubation in a CO2 incubator, the supernatant was collected and the alcohol concentration in the collected supernatant was detected using a kit. The alcohol concentration (vol%) of each group is shown in Table 4: Table 4

[0092] In Table 4, each group was compared with the model group: △ P<0.05, △△ P<0.01, with significant difference; the comparative example group was compared with the example 1 group: # P<0.05, ## P<0.01, there is a significant difference.

[0093] As can be seen from Table 4, the alcohol sobering and liver protecting composition of the present invention can quickly reduce the alcohol concentration. The alcohol concentration within 3 hours is reduced from 0.932 vol% to below 0.20 vol%, and the alcohol concentration is reduced to about 20% of the original. Compared with the comparative example, the sobering speed is significantly accelerated.

[0094] 2. Human hangover detoxification experiment Experimental process: the subjects were controlled before and after drinking the alcohol-detoxifying and liver-protecting composition. The test was performed on the first day of the experiment as a blank group; and the test was performed on the second day of the experiment as an experimental group after drinking the alcohol-detoxifying and liver-protecting composition.

[0095] Specific experimental steps: The subjects (30-40 years old) drank 50 mL of boiled water on the first day and used a breath alcohol concentration detector to test it to confirm that the basic value of the breath alcohol test before drinking was 0.00; then the subjects drank 75 mL of 40% whiskey within 10 minutes, rinsed their mouths with water twice to remove excess alcohol in their mouths, and tested it again with a breath alcohol detector 30 minutes after drinking, and the alcohol value was recorded (recorded as the alcohol value of the blank group).

[0096] The same subject drank 50 mL of the alcohol-relieving and liver-protecting composition (Example 1) on the second day, and tested it using a breath alcohol concentration detector to confirm that the basic value of the breath alcohol test before drinking was 0.00; then the subject drank 75 mL of 40% whiskey within 10 minutes, rinsed his mouth with water twice to remove excess alcohol in his mouth, and tested it again with a breath alcohol detector 30 minutes after drinking, and recorded the alcohol value (recorded as the alcohol value of the experimental group).

[0097] Note: During the test, the amount, time and type of meals should be consistent; during the test, only the specified water or products can be consumed, and no other drinks or foods are consumed.

[0098] The alcohol levels of each subject on the first and second days are shown in Table 5 .

[0099] Table 5

[0100] From the above experiments, it can be seen that the six components of the alcohol-detoxifying and liver-protecting composition of the present invention complement each other and are synergistically matched in a specific ratio, which has significant effects on metabolizing alcohol, protecting the liver, and increasing NAD + Content, reducing the content of MDA, AST and ALT.

[0101] Each component in the alcohol sobering and liver protecting composition of the present invention is indispensable and cannot be replaced at will. The results of Comparative Examples 3 and 4 show that even if replaced with other components with sobering effects, the significant sobering and liver protecting effects of the present invention cannot be achieved. In addition, the turmeric extract and licorice extract in the alcohol sobering and liver protecting composition of the present invention are obtained by specific preparation methods. The preparation method of turmeric extract is: using alkaline solution for reflux-assisted ultrasonic treatment twice, which can significantly improve the activity and content of the effective ingredients; the efficient retention of curcumin can significantly remove free radicals, improve the ability and speed of sobering up, and achieve the function of protecting the liver and cardiovascular system.

[0102] Licorice extract is prepared by a gradient heating method to avoid the reduction of the activity of the effective ingredients caused by high temperature. Turmeric extract and licorice extract work synergistically to accelerate the decomposition of alcohol and improve liver protection function.

[0103] Experiment 3 Stability test of alcohol-relieving and liver-protecting oral solution The stability of the alcohol-relieving and liver-protecting oral liquids prepared by Application Example 1 to Application Example 3 and Application Comparative Example 1 to Application Comparative Example 3 was tested.

[0104] Each alcohol-relieving and liver-protecting oral liquid was subjected to an accelerated test (simulating commercial packaging, temperature 40°C ± 2°C, relative humidity 75% ± 5%, and placed for 6 months). The results of the property observation experiment are shown in Table 6.

[0105] Table 6

[0106] As can be seen from Table 6, the hangover-detoxifying and liver-protecting oral liquid prepared by combining the hangover-detoxifying and liver-protecting composition of the present invention with xanthan gum, gum arabic and sorbitol has a high stability; compared with the oral liquid obtained by mixing with other auxiliary materials, the oral liquid of the present invention has a more stable performance and does not change in properties after long-term storage. However, after the components of the auxiliary materials were changed in Comparative Examples 1 to 3, the oral liquids all changed color at high temperatures, which was not conducive to storage.

[0107] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions of the technical solution of the present invention by ordinary technicians in this field do not deviate from the essence and scope of the technical solution of the present invention.

Claims

1. A composition for sobering up and protecting the liver, characterized in that: The composition is composed of the following ingredients by weight: 50-200 parts of Ampelopsis grossedentata leaf instant powder, 1-5 parts of turmeric extract, 2-8 parts of corn oligopeptide powder, 2-8 parts of Hovenia dulcis powder, 1.4-7 parts of artichoke powder and 0.2-1 parts of licorice extract; The preparation method of turmeric extract comprises the following steps: mixing turmeric with water, adjusting the pH to 8.0-9.5, performing a first ultrasonic treatment; then heating and refluxing extraction at 75-90° C. for 4-7 hours; and finally performing a second ultrasonic treatment and filtering to obtain the turmeric extract; The preparation method of licorice extract comprises the steps of: mixing licorice powder with hot water at 45-55 DEG C for the first extraction; then heating to 65-75 DEG C for the second extraction; heating to 80-85 DEG C for the third extraction, and filtering to obtain the licorice extract.

2. The alcohol sobering and liver protecting composition according to claim 1, characterized in that: The invention is composed of the following ingredients by weight: 100-150 parts of Ampelopsis grossedentata leaf instant powder, 2-4 parts of turmeric extract, 4-6 parts of corn oligopeptide powder, 4-7 parts of Hovenia dulcis powder, 2.5-5.5 parts of artichoke powder and 0.5-0.8 parts of liquorice extract.

3. The alcohol sobering and liver protecting composition according to claim 2, characterized in that: The invention is composed of the following ingredients by weight: 130 parts of Ampelopsis grossedentata leaf instant powder, 3 parts of turmeric extract, 5 parts of corn oligopeptide powder, 5 parts of Hovenia dulcis powder, 4 parts of artichoke powder and 0.7 parts of licorice extract.

4. The alcohol sobering and liver protecting composition according to claim 1, characterized in that: The preparation method of the turmeric extract comprises the steps of: (1) crushing turmeric to a particle size of 40-80 mesh, mixing with water at a solid-liquid ratio of 1:15-25 g / mL, adjusting the pH to 8.5-9.0, and performing a first ultrasonic treatment for 20-40 minutes to obtain a first ultrasonic treatment mixture; (2) subjecting the first ultrasonic treatment mixture of step (1) to reflux extraction at 80-90° C. for 5-6 hours to obtain a reflux extraction mixture; (3) subjecting the reflux extraction mixture of step (2) to a second ultrasonic treatment for 30-50 min, and filtering to obtain a turmeric extract; (4) Concentrating and drying the turmeric extract obtained in step (3) to obtain a turmeric extract.

5. The alcohol sobering and liver protecting composition according to claim 4, characterized in that: The preparation method of the turmeric extract comprises the steps of: (1) Grinding turmeric to a particle size of 60 mesh, mixing with water at a solid-liquid ratio of 1:20 g / mL, adjusting the pH to 8.8, and performing a first ultrasonic treatment for 30 min to obtain a first ultrasonic treatment mixture; (2) subjecting the first ultrasonic treatment mixture of step (1) to reflux extraction at 85° C. for 5 h to obtain a reflux extraction mixture; (3) subjecting the reflux extraction mixture of step (2) to a second ultrasonic treatment for 40 min, and filtering to obtain a turmeric extract; (4) Concentrating and drying the turmeric extract obtained in step (3) to obtain a turmeric extract.

6. The alcohol sobering and liver protecting composition according to claim 1, characterized in that: The preparation method of the licorice extract comprises the steps of: S1, mixing licorice powder with a particle size of 40-80 mesh with hot water at 45-50° C. at a solid-to-liquid ratio of 1:15-25 g / mL, stirring for a first extraction for 0.5-2h, and obtaining a first extraction mixture; S2, heating the first extraction mixture of step S1 to 65-70° C., stirring and performing a second extraction for 2-5 hours to obtain a second extraction mixture; S3, heating the second extraction mixture of step S2 to 80-85°C, stirring for a third extraction for 0.5-2h, and filtering to obtain a licorice extract; S4, concentrating and drying the liquorice extract obtained in step S3 to obtain a liquorice extract.

7. The alcohol sobering and liver protecting composition according to claim 6, characterized in that: The preparation method of the licorice extract comprises the steps of: S1, licorice powder with a particle size of 60 mesh, mixed with 50°C hot water at a solid-liquid ratio of 1:20 g / mL, stirred for the first extraction for 1 hour, and obtained a first extraction mixture; S2, heating the first extraction mixture of step S1 to 70° C., stirring and performing a second extraction for 3 h to obtain a second extraction mixture; S3, heating the second extraction mixture of step S2 to 85° C., stirring for a third extraction for 1 hour, and filtering to obtain a licorice extract; S4, concentrating and drying the liquorice extract obtained in step S3 to obtain a liquorice extract.

8. The method for preparing the alcohol sobering and liver protecting composition according to any one of claims 1 to 7, characterized in that: The method comprises the following steps: screening the instant powder of Ampelopsis grossedentata leaves, Hovenia dulcis powder and artichoke powder with a particle size of 80-150 meshes; mixing and stirring the instant powder of Ampelopsis grossedentata leaves, corn oligopeptide powder and licorice extract to obtain a mixture 1; mixing and stirring the turmeric extract, Hovenia dulcis powder and artichoke powder to obtain a mixture 2; and stirring the mixture 1 and the mixture 2 to obtain a hangover-relief and liver-protecting composition.

9. Use of the alcohol sobering and liver protecting composition according to any one of claims 1 to 7 in food preparation.

10. An oral liquid for sobering up and protecting the liver, characterized in that: The ingredients include, by weight: 10-30 parts of the alcohol sobering and liver protecting composition according to any one of claims 1-7, 1-4 parts of xanthan gum, 0.3-1.5 parts of gum arabic, 4-7 parts of sorbitol and 300-500 parts of water; The preparation method of the alcohol-relieving and liver-protecting oral liquid comprises the steps of: ①, after mixing the alcohol-detoxifying and liver-protecting composition with xanthan gum and water, perform high-pressure homogenization at 2-5 MPa to obtain a homogeneous mixture; ②. Mix the homogenized mixture of step ① with gum arabic and sorbitol, and homogenize under high pressure of 6-8 MPa to obtain an oral liquid for alcohol sobering up and protecting the liver.

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