Chinese mugwort plant extraction antibacterial foaming agent and preparation method thereof

Abstract

The invention provides a Chinese mugwort herb plant extraction antibacterial foaming agent and a preparation method thereof, and belongs to the technical field of Chinese herbal medicine lotions, the Chinese mugwort herb plant extraction antibacterial foaming agent comprises the following components by weight: 10%-20% of a Chinese mugwort extract, 0.1%-5% of Chinese mugwort oil, 2%-5% of a gentian root extract, 2%-5% of a sophora flavescens extract, 2%-5% of a scutellaria baicalensis extract, 2%-5% of a rhizoma smilacis glabrae extract, 0.2%-1% of cinnamon oil, 4%-20% of a cosolvent, 2%-10% of a humectant, 12%-40% of a surfactant, 0.2%-0.6% of a preservative, 0.5%-5% of a pH regulator, and the balance of water. And 30%-60% of water. According to the foaming agent, Chinese mugwort, radix gentianae, radix sophorae flavescentis, radix scutellariae, rhizoma smilacis glabrae, cortex cinnamomi and other plants serve as main raw materials, effective components such as flavone, gentiopicroside, sophocarpidine and baicalin are extracted respectively, menthol and a potassium cocoyl glycinate amino acid surfactant and the like are compounded, the foaming agent has the killing effect on staphylococcus aureus, escherichia coli and candida albicans, and the foaming agent is free of toxic and side effects. And the nursing effects of cleaning, decontamination, peculiar smell removal, antibiosis and the like can be completed on the nursed part within several minutes.

Description

Technical Field

[0001] The present application relates to the technical field of Chinese herbal lotions, and in particular to an antibacterial foaming agent containing Qi Ai herbal plant extract and a preparation method thereof. Background Art

[0002] Common gynecological diseases such as vulvitis and vaginitis are mostly caused by bad hygiene habits and poor sanitary conditions. The active ingredients of common antibacterial washes on the market are chlorhexidine, metronidazole and other ingredients, which have strong antibacterial ability, but are not suitable for daily care.

[0003] Qi Artemisia argyi is a local medicinal material of Artemisia argyi, which is pungent, bitter and warm. It enters the liver, spleen and kidney meridians. It warms the meridians and stops bleeding, dispels cold and relieves pain; it can be used externally to remove dampness and relieve itching. Modern pharmacological studies have shown that Artemisia argyi and its extracts have pharmacological effects such as antibacterial, antiviral, antioxidant, liver-protecting and choleretic, hemostatic and anticoagulant, antiallergic, immunomodulatory and anticancer. The Chinese invention patent with application number 20181123368.7 discloses an external Chinese medicine lotion for treating gynecological inflammation, and the Chinese invention patent with application number 201610863931.3 discloses a gynecological Chinese medicine lotion. These patents all contain Artemisia argyi, which is made from the filtrate after decoction of Chinese medicinal materials such as Artemisia argyi. The filtrate obtained after decoction of Artemisia argyi contains more impurities soluble in water, and loses more water-insoluble components, making it difficult to exert the full efficacy of Qi Artemisia argyi; the lotions in the above patents are all aqueous, which need to be used in a sitting bath or other ways, which is inconvenient to use. Summary of the invention

[0004] In view of the technical problems existing in the background technology, the present application provides an antibacterial foam agent made from Qi Ai herbal plant extract and a preparation method thereof, aiming to solve the technical problems of cleaning, decontamination, deodorization and antibacterial of female care parts.

[0005] In the first aspect, the embodiments of the present application provide an antibacterial foam of Qi Ai herbal plant extract, comprising the following components in weight percentage: Qi Ai extract 10%~20%, Qi Ai oil 0.1%~5%, gentian root extract 2%~5%, Sophora flavescens extract 2%~5%, Scutellaria baicalensis extract 2%~5%, Smilax glabra extract 2%~5%, cinnamon oil 0.2%~1%, solubility enhancer 4%~20%, moisturizer 2%~10%, surfactant 12%~40%, preservative 0.2%~0.6%, pH adjuster 0.5%~5%, water 30%~60%.

[0006] Preferably, the Artemisia selengensis oil is prepared by the following steps: S1. Wash and chop the stems of Artemisia argyi, place them in a cold storage at ~15°C for 30-40 minutes, take them out and immediately place them in a 15% sodium hydroxide aqueous solution at 0-10°C, soak them for 45-60 minutes, then heat the solution until it boils, keep it warm for 60-90 seconds, and filter to obtain a precipitate; S2, washing the precipitate with clean water until the pH value is between 8.5 and 9.5, and then using a composite enzyme solution to fully enzymolyze at 35 to 40° C. for 200 to 220 minutes to obtain a blend of the enzymolyzed precipitate and the composite enzyme solution; S3, adding water to the blend, and extracting by steam distillation to obtain Qi Ai oil.

[0007] Preferably, the complex enzyme comprises at least two of alkaline protease, cellulase, hemicellulase and pectinase.

[0008] Preferably, the Artemisia argyi extract is prepared by the following steps: S1. After cleaning the leaves of Artemisia argyi, soak them in clean water for 30 to 40 minutes, use ultrasonic treatment for 5 to 10 seconds every 5 minutes during the soaking period, and then use steam distillation to distill them to obtain fractions and substrates, and the fraction is Artemisia argyi oil; S2, concentrate the substrate under reduced pressure, then add 8 to 12 times the volume of the substrate to precipitate with ethanol solution for 24 to 48 hours, with the concentration of the ethanol solution being 80% to 90%; S3. After the alcohol precipitation is completed, the mixture is filtered to separate the supernatant from the impurities, the supernatant is heated, and the ethanol in the supernatant is recovered to obtain the Artemisia argyi extract.

[0009] Preferably, the cosolvent is one or both of ethanol and PEG-40 hydrogenated castor oil.

[0010] Preferably, the surfactant includes 10% to 30% potassium cocoyl glycinate and 2% to 10% cocamidopropyl betaine.

[0011] Preferably, the preservative is one or both of chlorphenesin and methylparaben.

[0012] Preferably, the pH adjuster is lactic acid.

[0013] Preferably, it also includes 0.1% to 0.3% menthol and 0.1% to 0.3% borneol.

[0014] In a second aspect, the present application provides a method for preparing an antibacterial foam of Qi Ai herbal plant extract, comprising the following steps: S1. Mix water, glycerin and preservative and heat to 80°C, add surfactant and stir evenly, cool to 45°C to obtain a first mixed solution; S2, stirring the cosolvent, Artemisia argyi oil, menthol and cinnamon oil to obtain a second mixed solution; S3, adding the second mixed solution into the first mixed solution, stirring and mixing evenly, to obtain a third mixed solution; S4. Add the Artemisia argyi extract, the gentian root extract, the Sophora flavescens extract, the Astragalus extract, the Smilax glabra extract and the pH adjuster to the third mixed solution, stir evenly to obtain a foaming agent.

[0015] Different from the existing technical solutions, the beneficial effects of this application include: The foaming agent in the present application uses Qi Ai, Gentiana, Sophora flavescens, Scutellaria baicalensis, Smilax glabra, cinnamon and other plants as main raw materials, and extracts their effective ingredients such as flavonoids, gentiopicroside, matrine, baicalin, etc., and compounded with menthol, potassium cocoyl glycinate amino acid surfactant, etc., which have a killing effect on Staphylococcus aureus (ATCC6538), Escherichia coli (8099), and Candida albicans (ATCC10231), and can complete cleaning, decontamination, odor removal, antibacterial and other care effects on the cared area within a few minutes. The formula of this application selects Chinese herbal ingredients and is matched with scientific extraction and purification technology to obtain the purest plant essence, including Qi Ai extract, Qi Ai oil, gentian oil, sophora flavescens oil, scutellaria oil, smilax glabra oil and cinnamon oil. The sources of these essential oils and extracts have fixed planting areas, adopt scientific and efficient extraction technology, strict quality control standards, high quality batch stability, and complete MSDS, origin certificate, COA and efficacy data. After repeated experiments, the best ratio was obtained and passed the stability and safety tests. The stability, sterilization experiments (2min, 5min, 10min, 20min for Escherichia coli, Staphylococcus aureus, Candida albicans) and multiple mucosal irritation tests of the obtained antibacterial foam agent all meet the requirements of relevant regulations. The foam agent in this application is mainly aimed at the needs of young women in the new era, and combines antibacterial deodorization, anti-inflammatory and itching, warming the uterus and consolidating the foundation, and washing and nursing. Herbal formula, for external washing and internal conditioning, the product pH value is 4.5~5.5, simulating the pH value, and does not damage the pH value of the vaginal mucosa. The dilution-free stock solution formula only requires light pressure on the pump head, and the stock solution will become foamy. Gently rub the foam on the area that needs care for a while, and then wash it off with clean water. It is easy to use. The above description is only an overview of the technical solution of the present application. In order to more clearly understand the technical means of the present application, it can be implemented in accordance with the contents of the specification, and in order to make the above and other purposes, features and advantages of the present application more obvious and easy to understand, the specific implementation methods of the present application are listed below. DETAILED DESCRIPTION

[0016] The following is a detailed description of the embodiments of the technical solution of the present application. The following embodiments are only used to more clearly illustrate the technical solution of the present application, and are therefore only used as examples, and cannot be used to limit the scope of protection of the present application.

[0017] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by technicians in the technical field to which this application belongs; the terms used herein are only for the purpose of describing specific embodiments and are not intended to limit this application; the terms "including" and "having" in the description and claims of this application and any variations thereof are intended to cover non-exclusive inclusions.

[0018] Reference to "embodiments" herein means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present application. The appearance of the phrase in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0019] In the description of the embodiments of the present application, the term "and / or" is only a description of the association relationship of the associated objects, indicating that there may be three relationships. For example, A and / or B can represent: A exists alone, A and B exist at the same time, and B exists alone. In addition, the character " / " in this article generally indicates that the associated objects before and after are in an "or" relationship.

[0020] In the description of the embodiments of the present application, the term "multiple" refers to more than two (including two). Similarly, "multiple groups" refers to more than two groups (including two groups), and "multiple pieces" refers to more than two pieces (including two pieces).

[0021] The active ingredients of common antibacterial washes on the market are chlorhexidine, metronidazole and other ingredients, which have strong antibacterial ability, but are not suitable for daily care.

[0022] In order to solve the technical problems of cleaning, decontamination, odor removal and antibacterial of female care parts, the present application provides an antibacterial foam agent of Qi Ai herbal plant extract and a preparation method thereof, wherein, by using Qi Ai, Gentiana, Sophora flavescens, Scutellaria baicalensis, Smilax glabra, cinnamon and other plants as main raw materials, respectively extracting their effective ingredients such as flavonoids, gentiopicroside, matrine, baicalin, etc., and compounding menthol, potassium cocoyl glycinate amino acid surfactant, etc., it can have a killing effect on Staphylococcus aureus (ATCC6538), Escherichia coli (8099), and Candida albicans (ATCC10231), and can complete cleaning, decontamination, odor removal, antibacterial and other care effects on the care parts within a few minutes.

[0023] In the first aspect, the embodiments of the present application provide an antibacterial foam of Qi Ai herbal plant extract, comprising the following components in weight percentage: Qi Ai extract 10%~20%, Qi Ai oil 0.1%~5%, gentian root extract 2%~5%, Sophora flavescens extract 2%~5%, Scutellaria baicalensis extract 2%~5%, Smilax glabra extract 2%~5%, cinnamon oil 0.2%~1%, solubility enhancer 4%~20%, moisturizer 2%~10%, surfactant 12%~40%, preservative 0.2%~0.6%, pH adjuster 0.5%~5%, water 30%~60%.

[0024] In the technical solution of the embodiment of the present application: Qi Aiye: A local medicinal material of wormwood, pungent, bitter and warm. It enters the liver, spleen and kidney meridians. It warms the meridians and stops bleeding, dispels cold and relieves pain; it can be used externally to remove dampness and relieve itching. Modern pharmacological studies have shown that wormwood and its extracts have antibacterial, antiviral, antioxidant, liver-protecting and choleretic, hemostatic and anticoagulant, antiallergic, immunomodulatory and anticancer pharmacological effects. Qi Aiye oil is extracted from the steam distillation of fresh Qi Aiye picked around the Dragon Boat Festival every year. The main components are ethyl acetate, 1.8-eucalyptol, hydrated camphene, camphor, 2-terpineol, caryophyllene, thujone and iso-thujone, etc. It has the effects of dispelling cold, removing dampness, relieving pain, antibacterial, anti-inflammatory, activating collaterals, warming the meridians, purifying and repairing the skin, anti-aging, and scraping.

[0025] Qi Ai Extract: After extracting the oil from Qi Ai leaves, heat and reflux to extract, add 8 to 12 times the volume of 80% to 90% ethanol solution and precipitate for 24 to 48 hours after decompression and concentration, recover the ethanol and then perform elution to obtain Qi Ai Extract. The main components include flavonoids, polysaccharides, chlorogenic acid, etc., which have anti-allergic, antibacterial and antiviral, and meridian warming effects.

[0026] Gentiana: Gentiana is bitter and cold in nature. It enters the liver and gallbladder meridians. It has the functions of clearing away heat and dampness, and purging liver and gallbladder fire. It is used for damp-heat jaundice, vaginal swelling and itching, leucorrhea, eczema and itching, liver-fire red eyes, tinnitus and deafness, flank pain and bitter mouth, strong middle, convulsions. Gentiana root extract is prepared by adding the medicinal material to a butanediol aqueous solution, using ultrasound-assisted and heated extraction, filtering after cooling, decolorizing and deodorizing the filtrate, distilling under reduced pressure, and then eluting and analyzing with ethanol to obtain a gentiopicroside extract. The main component is gentiopicroside, which has antibacterial, anti-inflammatory, analgesic, and antiviral effects.

[0027] Sophora flavescens: Sophora flavescens is bitter and cold. It enters the heart, liver, stomach, large intestine, and bladder meridians. It has the functions of clearing away heat and dampness, killing insects, and promoting diuresis. It is used for heat dysentery, blood in stool, jaundice and urine retention, leucorrhea, vaginal swelling and itching, eczema, wet sores, skin itching, scabies and leprosy; it is also used for external treatment of trichomonas vaginitis. The extract of Sophora flavescens is extracted by ultrasonic extraction and then centrifuged to obtain a Sophora flavescens extract with a high content of matrine. The main component is matrine, which has anti-tumor, anti-inflammatory, antibacterial, and immunomodulatory effects.

[0028] Scutellaria baicalensis: bitter and cold in nature. Enters the lung, gallbladder, spleen, large intestine and small intestine meridians. Functions: clearing away heat and dampness, purging fire and detoxifying, stopping bleeding and calming the fetus. Used for damp-heat, summer-heat, chest tightness and nausea, damp-heat fullness, diarrhea, jaundice, lung heat cough, high fever and thirst, blood heat vomiting and bleeding, carbuncle, sore and toxicity, and restless fetus. Scutellaria baicalensis extract is obtained by water extraction, acid-base treatment, ethanol precipitation and decompression drying. It contains baicalin, which has anti-tumor, anti-pathogen and anti-allergic effects.

[0029] Smilax glabra: sweet, light, and neutral in nature. It enters the liver and stomach meridians. It has the functions of detoxification, dehumidification, and joint dredge. The main component of Smilax glabra extract is astilbin. It has anti-tumor, antiviral, antibacterial, and dehumidification effects.

[0030] Cinnamon: pungent, sweet, and very hot. It enters the kidney, spleen, heart, and liver meridians. It has the functions of replenishing fire and supporting yang, and guiding fire back to the origin. It dispels cold and relieves pain, and warms and unclogs the meridians. The main component of cinnamon volatile oil is cinnamaldehyde, which has the functions of warming the middle, dispelling cold, regulating qi, and relieving pain.

[0031] Preferably, the Artemisia selengensis oil is prepared by the following steps: S1. Wash and chop the stems of Artemisia argyi, place them in a cold storage at ~15°C for 30-40 minutes, take them out and immediately place them in a 15% sodium hydroxide aqueous solution at 0-10°C, soak them for 45-60 minutes, then heat the solution until it boils, keep it warm for 60-90 seconds, and filter to obtain a precipitate; S2, washing the precipitate with clean water until the pH value is between 8.5 and 9.5, and then using a composite enzyme solution to fully enzymolyze at 35 to 40° C. for 200 to 220 minutes to obtain a blend of the enzymolyzed precipitate and the composite enzyme solution; S3, adding water to the blend, and extracting by steam distillation to obtain Qi Ai oil.

[0032] In the technical scheme of the embodiment of the present application: the present application first freezes and treats the stems of Artemisia argyi with sodium hydroxide to dissolve the epidermal wax silicon layer of the stems of Artemisia argyi, shrinks the thin walls, thick walls and vascular tissues, and accelerates the degradation of these tissues. At the same time, it can also reduce the degree of lignification of the stems of Artemisia argyi, weaken the covalent connection between cellulose and hemicellulose, swell the cellulose and expose its ultrastructure, which is conducive to the contact between enzymes and cellulose; then use specific enzymes to pretreat the plant raw materials, destroy the structure of the plant cell walls, and release the essential oil components in the cells. This step helps to fully extract the essential oil components in the subsequent steam distillation process without destroying the effective ingredients of the essential oils.

[0033] Steam distillation is a method of using the high temperature and high pressure of steam to distill the essential oil components in plant materials together with the steam, and then obtain the essential oil through condensation and separation steps. This method is simple to operate, has high extraction efficiency, and can better preserve the flavor and aroma of the essential oil.

[0034] Extracting plant essential oils with steam after enzymatic hydrolysis is an efficient and environmentally friendly method for extracting essential oils.

[0035] Preferably, the complex enzyme comprises at least two of alkaline protease, cellulase, hemicellulase and pectinase.

[0036] Preferably, the Artemisia argyi extract is prepared by the following steps: S1. After cleaning the leaves of Artemisia argyi, soak them in clean water for 30 to 40 minutes, use ultrasonic treatment for 5 to 10 seconds every 5 minutes during the soaking period, and then use steam distillation to distill them to obtain fractions and substrates, and the fraction is Artemisia argyi oil; S2, concentrate the substrate under reduced pressure, then add 8 to 12 times the volume of the substrate to precipitate with ethanol solution for 24 to 48 hours, with the concentration of the ethanol solution being 80% to 90%; S3. After the alcohol precipitation is completed, the mixture is filtered to separate the supernatant from the impurities, the supernatant is heated, and the ethanol in the supernatant is recovered to obtain the Artemisia argyi extract.

[0037] In the technical scheme of the embodiment of the present application: first, ultrasonic waves are used to treat the Qi Ai leaves, which can rupture the cell walls of the Qi Ai leaves and release the essential oil components in the cells, which is beneficial to the subsequent extraction of Qi Ai oil, and other substances will not be introduced into the Qi Ai leaves, which is convenient for the subsequent preparation of Qi Ai extract. The substrate after the Qi Ai oil is extracted by steam distillation also contains a large number of effective ingredients, the main components of which are flavonoids, polysaccharides, chlorogenic acid, etc. This application adopts the method of alcohol precipitation, which utilizes the characteristics that the effective ingredients can be dissolved in ethanol while the impurities are insoluble in ethanol. By adding an appropriate amount of ethanol to the substrate, the effective ingredients are dissolved in ethanol, and the impurities are precipitated, thereby achieving the purpose of removing impurities and retaining the effective ingredients of the drug.

[0038] Preferably, the cosolvent is one or both of ethanol and PEG-40 hydrogenated castor oil.

[0039] Preferably, the surfactant includes 10% to 30% potassium cocoyl glycinate and 2% to 10% cocamidopropyl betaine.

[0040] In the technical solution of the embodiment of the present application: a co-solvent is added to dissolve the Artemisia argyi oil and cinnamon oil in water, and then a surfactant is used to prepare a foaming agent for easy use by consumers.

[0041] Preferably, the preservative is one or both of chlorphenesin and methylparaben.

[0042] Preferably, the pH adjuster is lactic acid.

[0043] Preferably, it also includes 0.1% to 0.3% menthol and 0.1% to 0.3% borneol.

[0044] In a second aspect, the present application provides a method for preparing an antibacterial foam of Qi Ai herbal plant extract, comprising the following steps: S1. Mix water, glycerin and preservative and heat to 80°C, add surfactant and stir evenly, cool to 45°C to obtain a first mixed solution; S2, stirring the cosolvent, Artemisia argyi oil, menthol and cinnamon oil to obtain a second mixed solution; S3, adding the second mixed solution into the first mixed solution, stirring and mixing evenly, to obtain a third mixed solution; S4. Add the Artemisia argyi extract, the gentian root extract, the Sophora flavescens extract, the Astragalus extract, the Smilax glabra extract and the pH adjuster to the third mixed solution, stir evenly to obtain a foaming agent.

[0045] In the technical solution of the embodiment of the present application: the foam agent obtained in the present application is pressed into dense foam through the foam bottle pump head, which can effectively have the effects of antibacterial and deodorizing, anti-inflammatory and antipruritic, warming the uterus and strengthening the foundation.

[0046] Some specific embodiments are listed below. It should be noted that the embodiments described below are exemplary and are only used to explain the present application, and should not be construed as limiting the present application. If specific techniques or conditions are not specified in the embodiments, the techniques or conditions described in the literature in this area or the product specifications are used. The reagents or instruments used without specifying the manufacturer are all conventional products that can be obtained commercially.

[0047] 1. Preparation method Example 1 A foaming agent of Qi Ai herbal plant extract antibacterial, Qi Ai extract 10%, Qi Ai oil 0.5%, gentian root extract 2%, sophora flavescens extract 2%, scutellaria baicalensis extract 2%, smilax glabra extract 2%, cinnamon oil 0.2%, ethanol 2%, glycerol 2%, PEG~40 hydrogenated castor oil 4%, water 52%, menthol 0.3%, borneol 0.3%, potassium cocoyl glycinate 10%, cocamidopropyl betaine 5%, chlorphenesin 0.4%, methylparaben 0.3%, lactic acid 5%. In this embodiment, gentian root extract, sophora flavescens extract, scutellaria baicalensis extract, cinnamon oil, and smilax glabra extract are all purchased finished products, and Qi Ai extract and Qi Ai oil are prepared by the following method: Qi Ai oil is prepared by the following steps: S1. Wash and chop the stems of Artemisia argyi, place them in a cold storage at ~15°C for 40 minutes, take them out and immediately place them in a 15% sodium hydroxide aqueous solution at 0°C for 60 minutes, then heat the solution until it boils, keep it warm for 60 seconds, and filter to obtain a precipitate; S2, the precipitate was rinsed with clean water until the pH value was between 8.5 and 9.5, and then 2.0 U / mg of alkaline protease, 5.0 U / mg of cellulase, and 2.0 U / mg of hemicellulase were used for full enzymolysis at 40°C for 220 minutes to obtain a precipitate after enzymolysis and a blend of a composite enzyme solution; S3, adding water to the blend, and extracting by steam distillation to obtain Qi Ai oil.

[0048] The extract of Artemisia argyi is prepared by the following steps: S1. After cleaning the leaves of Artemisia argyi, soak them in clean water for 40 minutes, use ultrasonic treatment for 5 seconds every 5 minutes during the soaking period, and then use steam distillation to distill them to obtain fractions and substrates, and the fraction is Artemisia argyi oil; S2. Concentrate the substrate under reduced pressure, then add 12 times the volume of the substrate to ethanol solution for alcohol precipitation 24, the ethanol concentration is 90%, filter to separate the supernatant and impurities, and recover the ethanol in the supernatant to obtain the Artemisia argyi extract.

[0049] A method for preparing an antibacterial foaming agent containing Qi Ai herbal plant extracts comprises the following steps: S1, mixing water, glycerin and chlorphenesin and heating to 80°C, adding potassium cocoyl glycinate and cocamidopropyl betaine and stirring evenly, cooling to 45°C to obtain a first mixed solution; S2, stirring ethanol, PEG-40 hydrogenated castor oil, Artemisia selengensis oil, menthol and cinnamon oil to obtain a second mixed solution; S3, adding the second mixed solution into the first mixed solution, stirring and mixing evenly, to obtain a third mixed solution; S4. Add the Artemisia argyi extract, the gentian root extract, the Sophora flavescens extract, the Astragalus extract, the Smilax glabra extract and lactic acid to the third mixed solution, stir evenly to obtain a foaming agent.

[0050] Example 2 The difference between Example 2 and Example 1 is that the formula of the foaming agent of Qi Ai herbal plant extract antibacterial agent is different, and the preparation method is the same. The formula of the foaming agent of Qi Ai herbal plant extract antibacterial agent in Example 2 is as follows, including the following components in weight percentage: Qi Ai extract 15%, Qi Ai oil 1%, gentian root extract 3%, Sophora flavescens extract 3%, Scutellaria baicalensis extract 3%, Smilax glabra extract 3%, cinnamon oil 0.5%, ethanol 6%, glycerin 3%, PEG~40 hydrogenated castor oil 6%, water 30.7%, menthol 0.2%, borneol 0.2%, potassium cocoyl glycinate 15%, cocamidopropyl betaine 7%, chlorphenesin 0.2%, methylparaben 0.2%, lactic acid 3%.

[0051] Example 3 The difference between Example 3 and Example 1 is that the formula of the foaming agent of Qi Ai herbal plant extract antibacterial is different, and the preparation method is the same. The formula of the foaming agent of Qi Ai herbal plant extract antibacterial in Example 3 is as follows, including the following components in weight percentage: Qi Ai extract 20%, Qi Ai oil 2%, gentian root extract 2%, Sophora flavescens extract 2%, Scutellaria baicalensis extract 2%, Smilax glabra extract 5%, cinnamon oil 1%, ethanol 5%, glycerin 2%, PEG~40 hydrogenated castor oil 5%, water 31.65%, menthol 0.1%, borneol 0.1%, potassium cocoyl glycinate 10%, cocamidopropyl betaine 10%, chlorphenesin 0.15%, methylparaben 0.1%, lactic acid 2%.

[0052] 2. Test Method 1. The bactericidal experiment of Qi Ai herbal plant extract antibacterial foam agent, the experimental method is as follows: Antibacterial test operation steps (refer to GB 15979~2002 "Hygiene Standards for Disposable Sanitary Products") Experimental steps: (1) Cultivate LB medium, nutrient agar medium, PBS buffer and control solution, and sterilize them with high pressure steam; (2) Inoculate the sterilized LB medium with bacterial strains overnight, activate and increase the bacteria, and culture for 12 hours. The inoculated bacterial strains are Staphylococcus aureus, Escherichia coli, and Candida albicans; (3) Take out 100 μL of the LB medium containing the pre-cultured bacteria and add it to 5 mL of PBS buffer to prepare a bacterial suspension; (4) Take 4 test tubes of PBS control solution and the Qi Ai herbal plant extract antibacterial foam prepared in Examples 1 to 3, respectively, with 5 ml in each tube, and add 100 μL of the above bacterial suspension to each test tube. Start the timing and act for 2 min, 5 min, 10 min, and 20 min respectively. Then, take 0.5 ml of the sample after the action in the above test tubes and put it into 4.5 ml of PBS solution for dilution.

[0053] 2. Stability test, the test method is as follows: 1. Control group, no treatment; 2. Normal temperature test group, placed below 25°C for 13 weeks; 3. Heat resistance test group, placed at 40℃~48℃ for 13 weeks; 4. Cold resistance test group, placed at 5℃~15℃ for 13 weeks; 5. Alternating test group, the alternating time is every 12 hours, alternating between 5℃~15℃ and 48℃, with a cycle of 7 days, for a total of 13 weeks.

[0054] 3. Safety test, the test method is as follows: Subjects: A total of 40 subjects, including 10 males and 30 females, aged 22 to 45 years old, with an average age of 26±6 years old, met the voluntary inclusion criteria.

[0055] Spot test method: Select qualified patch test equipment, use the closed patch test method, cut the patch product containing the test sample into the size of the patch tester (about 50mm²) and place it in the patch tester, apply it to the back of the subject with a hypoallergenic tape, and remove the test sample after 24 hours. Observe the skin reaction at 0.5h, 24h, and 48h after removal, and set up a negative control (blank, no treatment) at the same time, and record the results according to the skin reaction grading standard of the skin closed patch test in Chapter 7 of the "Technical Specifications for Safety of Cosmetics".

[0056] The test samples were observed on the first day, first month, second month and third month after treatment, and the observation items were the appearance, color, smell and skin feel of the test samples.

[0057] III. Analysis of test results of various embodiments and comparative examples The antibacterial foam prepared in Examples 1 to 3 was subjected to a sterilization experiment, and the test results are shown in Tables 1 to 3 below: Table 1 The number of live Staphylococcus aureus colonies after the action of Qi Ai herbal plant extract antibacterial foam (unit: cfu)

[0058] Table 2 The number of viable Escherichia coli colonies after the action of Qi Ai herbal plant extract antibacterial foam (unit: cfu)

[0059] Table 3 The number of viable Candida albicans colonies after the action of Qi Ai herbal plant extract antibacterial foam (unit: cfu)

[0060] It can be seen from Tables 1 to 3 that the antibacterial foam agent of the present invention has an inhibitory effect on pathogenic bacteria such as Staphylococcus aureus, Escherichia coli and Candida albicans, and has a good antibacterial effect. Among them, the effect of the formulation according to Example 3 is the most obvious.

[0061] The stability test results of the Qi Ai herbal plant extract antibacterial foam prepared in Example 3 are shown in Table 4 below: Table 4 Stability test results of Example 3

[0062] As can be seen from Table 4, the Qi Ai herbal plant extract antibacterial foam prepared in the present application has good stability and is recommended to be stored in a cool and dark place.

[0063] The Qi Ai herbal plant extract antibacterial foam prepared in Example 3 was tested for safety, and the test results are shown in Table 5 below: Table 5 Safety test results of Example 3 and negative control

[0064] As can be seen from Table 5, the results of the human skin patch test showed that among the 40 subjects, 0 people had adverse skin reactions, indicating that the Qi Ai herbal plant extract antibacterial foam prepared in this application is highly safe.

[0065] It should be noted that the present application is not limited to the above-mentioned embodiments. The above-mentioned embodiments are only examples, and the embodiments having the same structure as the technical idea and exerting the same effect within the scope of the technical solution of the present application are all included in the technical scope of the present application. In addition, without departing from the scope of the main purpose of the present application, various modifications that can be thought of by those skilled in the art to the embodiments and other methods of combining some of the constituent elements in the embodiments are also included in the scope of the present application.

Claims

1. An antibacterial foaming agent containing Qi Ai herbal plant extract, characterized in that: The invention comprises the following components in weight percentage: 10%-20% of Qi Ai extract, 0.1%-5% of Qi Ai oil, 2%-5% of gentian root extract, 2%-5% of sophora flavescens extract, 2%-5% of scutellaria baicalensis extract, 2%-5% of smilax glabra extract, 0.2%-1% of cinnamon oil, 4%-20% of cosolvent, 2%-10% of moisturizer, 12%-40% of surfactant, 0.2%-0.6% of preservative, 0.5%-5% of pH adjuster and 30%-60% of water.

2. The antibacterial foaming agent of Qi Ai herbal plant extract according to claim 1, characterized in that: The described Artemisia argyi oil is prepared by the following steps: S1. Wash and chop the stems of Artemisia argyi, place them in a cold storage at ~15°C for 30-40 minutes, take them out and immediately place them in a 15% sodium hydroxide aqueous solution at 0-10°C, soak them for 45-60 minutes, then heat the solution until it boils, keep it warm for 60-90 seconds, and filter to obtain a precipitate; S2, washing the precipitate with clean water until the pH value is between 8.5 and 9.5, and then using a composite enzyme solution to fully enzymolyze for 200 to 220 minutes at 35 to 40° C. to obtain a blend of the enzymolyzed precipitate and the composite enzyme solution; S3, adding water to the blend, and extracting by steam distillation to obtain Qi Ai oil.

3. The antibacterial foaming agent of Qi Ai herbal plant extract according to claim 2, characterized in that: The complex enzyme comprises at least two of alkaline protease, cellulase, hemicellulase and pectinase.

4. The antibacterial foaming agent of Qi Ai herbal plant extract according to claim 1, characterized in that: The Artemisia argyi extract is prepared by the following steps: S1. After cleaning the leaves of Artemisia argyi, soak them in clean water for 30 to 40 minutes, use ultrasonic treatment for 5 to 10 seconds every 5 minutes during the soaking period, and then use steam distillation to distill them to obtain fractions and substrates, and the fraction is Artemisia argyi oil; S2, concentrate the substrate under reduced pressure, then add 8 to 12 times the volume of the substrate to precipitate with ethanol solution for 24 to 48 hours, with the concentration of the ethanol solution being 80% to 90%; S3. After the alcohol precipitation is completed, the mixture is filtered to separate the supernatant from the impurities, the supernatant is heated, and the ethanol in the supernatant is recovered to obtain the Artemisia argyi extract.

5. The antibacterial foaming agent of Qi Ai herbal plant extract according to claim 1, characterized in that: The cosolvent is one or two of ethanol and PEG-40 hydrogenated castor oil.

6. The antibacterial foaming agent of Qi Ai herbal plant extract according to claim 1, characterized in that: The surfactant comprises 10% to 30% potassium cocoyl glycinate and 2% to 10% cocamidopropyl betaine.

7. The antibacterial foaming agent of Qi Ai herbal plant extract according to claim 1, characterized in that: The preservative is one or both of chlorphenesin and methylparaben.

8. The antibacterial foaming agent of Qi Ai herbal plant extract according to claim 1, characterized in that: The pH adjuster is lactic acid.

9. The antibacterial foaming agent of Qi Ai herbal plant extract according to claim 1, characterized in that: It also includes menthol 0.1%~0.3% and borneol 0.1%~0.3%.

10. A method for preparing an antibacterial foaming agent of Qi Ai herbal plant extract according to any one of claims 1 to 9, characterized in that: The steps include: S1, mixing water, glycerin and preservatives, heating, adding surfactant, stirring evenly, cooling, and obtaining a first mixed solution; S2, stirring the cosolvent, Artemisia argyi oil, menthol and cinnamon oil to obtain a second mixed solution; S3, adding the second mixed solution into the first mixed solution, stirring and mixing evenly, to obtain a third mixed solution; S4. Add the Artemisia argyi extract, the gentian root extract, the Sophora flavescens extract, the Astragalus extract, the Smilax glabra extract and the pH adjuster to the third mixed solution, stir evenly to obtain a foaming agent.

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