A culture device and a culture method for anaerobic ammonia-oxidizing bacteria

Abstract

The present invention relates to the technical field of bacterial strain cultivation, and discloses a cultivation device and a cultivation method for anaerobic ammonia-oxidizing bacterial strains. The cultivation device comprises a carrier outer cylinder, which is arranged in a reactor; the outer wall of the carrier outer cylinder and the inner wall of the reactor form an external temperature control channel; a carrier inner cylinder, which is arranged in the carrier outer cylinder; the outer wall of the carrier inner cylinder and the inner wall of the carrier outer cylinder form a water flow channel; the carrier inner cylinder also has an internal temperature control channel arranged along its own axis; wherein the external temperature control channel, the internal temperature control channel and the water flow channel are configured as follows: the flow direction of water in the external temperature control channel and the internal temperature control channel is the same, and opposite to the flow direction of water in the water flow channel. The cultivation method is applied to the cultivation device. Through the above scheme, the present invention can solve the technical problems of delayed temperature regulation and easy temperature difference in the cultivation device in the related art.

Description

Technical Field

[0001] The present invention relates to the technical field of bacterial strain cultivation, and in particular to a cultivation device and a cultivation method for anaerobic ammonia oxidizing bacterial strains. Background Art

[0002] The invention patent with application number CN202110171101.5 discloses a device for enriching and culturing anaerobic ammonia-oxidizing bacteria. The device relies on a temperature control device located outside the culture device to adjust the temperature inside the culture device at the periphery. It takes a certain amount of time for the temperature to change from the outer biofilm carrier to the inner biofilm carrier, resulting in a delay in temperature regulation and a temperature difference between the inside and outside of the biofilm carrier during the regulation process, which affects the culture quality of the strain.

[0003] Therefore, providing a culture device and a culture method for anaerobic ammonia-oxidizing bacteria with uniform and timely temperature regulation is a technical problem that needs to be solved urgently by those skilled in the art. Summary of the invention

[0004] The present invention discloses a culture device and a culture method for anaerobic ammonia-oxidizing bacteria, so as to solve the technical problems of delayed temperature regulation and easy temperature difference existing in the culture device in the related art.

[0005] In order to solve the above problems, the present invention adopts the following technical solutions:

[0006] In a first aspect, a device for cultivating anaerobic ammonia-oxidizing bacteria is disclosed, comprising:

[0007] The carrier outer cylinder is arranged in the reactor; the outer wall of the carrier outer cylinder and the inner wall of the reactor form an external temperature adjustment channel;

[0008] The carrier inner cylinder is arranged in the carrier outer cylinder; the outer wall of the carrier inner cylinder and the inner wall of the carrier outer cylinder form a water flow channel; the carrier inner cylinder also has an inner temperature adjustment channel arranged along its own axial direction;

[0009] A cache box, the water inlet of the cache box is connected to the water outlet of the external temperature adjustment channel, the water outlet of the internal temperature adjustment channel and the water outlet of the water flow channel respectively; the water outlet of the cache box is connected to the water inlet of the external temperature adjustment channel and the water inlet of the internal temperature adjustment channel after passing through the temperature regulator;

[0010] The external temperature adjustment channel, the internal temperature adjustment channel and the water flow channel are configured such that the flow direction of the water in the external temperature adjustment channel and the internal temperature adjustment channel is the same and opposite to the flow direction of the water in the water flow channel.

[0011] In some embodiments, a first water distribution pan is further included, and the first water distribution pan is located above the external temperature adjustment channel;

[0012] The first water distribution tray has a plurality of first water outlet holes arranged in an annular shape on one side facing the external temperature adjustment channel, and the projections of the plurality of first water outlet holes along the axial direction of the reactor are located in the external temperature adjustment channel;

[0013] Alternatively, a first annular slit is provided on a side of the first water distribution tray facing the external temperature adjustment channel, and a projection of the first annular slit along the axial direction of the reactor is located in the external temperature adjustment channel.

[0014] In some embodiments, the apertures of the plurality of first water outlet holes gradually increase from the water inlet close to the first water distribution tray toward the water inlet far away from the first water distribution tray;

[0015] Alternatively, the gap of the first annular gap gradually increases from the direction close to the water inlet of the first water distribution tray to the direction away from the water inlet of the first water distribution tray.

[0016] In some embodiments, the first water distribution tray is disposed on the top of the carrier outer cylinder, and its outer wall is connected to the inner wall of the reactor;

[0017] A first sealing portion is also sleeved near the bottom of the carrier outer cylinder, and the outer wall of the first sealing portion is connected to the inner wall of the reactor;

[0018] The external temperature adjustment channel is the space between the first sealing portion and the first water distribution tray.

[0019] In some embodiments, a second water distribution pan is further included, and the second water distribution pan is located above the inner temperature adjustment channel;

[0020] The second water distribution tray has a plurality of second water outlet holes arranged in an annular shape on one side facing the inner temperature adjustment channel, and the axial projections of the plurality of second water outlet holes are located in the inner temperature adjustment channel;

[0021] Alternatively, a second annular slit is provided on a side of the second water distribution tray facing the inner temperature adjustment channel, and a projection of the second annular slit along the axial direction of the reactor is located in the inner temperature adjustment channel.

[0022] In some solutions, the apertures of the plurality of second water outlet holes gradually increase from the water inlet close to the second water distribution tray toward the water inlet far away from the second water distribution tray;

[0023] Alternatively, the gap of the second annular gap gradually increases from the direction close to the water inlet of the second water distribution tray to the direction away from the water inlet of the second water distribution tray.

[0024] In some solutions, the second water distribution tray is arranged on the top of the carrier inner cylinder and covers the inner temperature adjustment channel;

[0025] A second sealing portion is provided near the bottom of the inner temperature adjustment channel, and the outer wall of the second sealing portion is connected to the inner wall of the carrier inner cylinder;

[0026] The inner temperature adjustment channel is the space between the second sealing portion and the second water distribution tray.

[0027] In some solutions, a third water distribution tray is further included, and the third water distribution tray is located below the water flow channel;

[0028] A side of the third water distribution tray facing the water flow channel is provided with a plurality of third water outlet holes arranged in a ring shape, and projections of the plurality of third water outlet holes along the axial direction of the reactor are located in the water flow channel.

[0029] In some solutions, an aeration plate is further included, and the aeration plate is located below the water flow channel;

[0030] The aeration plate has a plurality of annularly arranged air outlet holes on one side facing the water flow channel, and the projections of the plurality of air outlet holes along the axial direction of the reactor are located in the water flow channel;

[0031] And / or, it also includes a mounting plate, and a plurality of third water outlet holes and a plurality of air outlet holes respectively pass through the mounting plate and are alternately arranged.

[0032] In a second aspect, a method for culturing anaerobic ammonia-oxidizing bacteria is disclosed, which is applied to the culturing device of the first aspect, and comprises the following steps:

[0033] Introducing sewage into the water flow channel so that the sewage flows along the water flow channel;

[0034] The hot water exchange flow is introduced into the external temperature adjustment channel and the internal temperature adjustment channel respectively, so that the hot water exchange flow flows along the external temperature adjustment channel and the internal temperature adjustment channel and is opposite to the flow direction of the sewage in the water flow channel;

[0035] The hot water exchange flow in the external temperature regulating channel and the hot water exchange flow in the internal temperature regulating channel perform heat exchange on the sewage in the water flow channel, so that the bacteria are cultured in the water flow channel.

[0036] The technical solution adopted by the present invention can achieve the following beneficial effects:

[0037] The culturing device of the anaerobic ammonia-oxidizing bacteria of the present application, in the process of culturing the bacteria, introduces sewage into the water flow channel so that the sewage flows along the water flow channel. The hot water exchange flow is introduced into the external temperature control channel and the internal temperature control channel respectively, so that the hot water exchange flow flows along the external temperature control channel and the internal temperature control channel, and is opposite to the flow direction of the sewage in the water flow channel. The sewage in the water flow channel is heat exchanged in the form of convection, and the temperature in the water flow channel is controlled at a temperature that is conducive to the reproduction of the bacteria. In the process of heat exchange of the sewage by the hot water exchange flow, the hot water exchange flow in the external temperature control channel exchanges heat with the sewage from the outside to the inside, and the hot water exchange flow in the internal temperature control channel exchanges heat with the sewage from the inside to the outside, thereby making the temperature regulation response of the sewage fast and not prone to temperature differences. The hot water exchange flow flowing out of the external temperature control channel and the internal temperature control channel and the sewage in the water flow channel can enter the cache box. After being heated by the temperature regulator, they can enter the external temperature control channel and the internal temperature control channel again as the hot water exchange flow to exchange heat with the sewage in the water flow channel. Since the hot water exchange flow flowing out of the external temperature control channel and the internal temperature control channel and the sewage in the water flow channel themselves have a certain amount of heat and have similar temperatures, they only need to be heated to a small extent by the temperature regulator before they can be used as the hot water exchange flow again, which can significantly reduce energy consumption. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0039] Figure 1 It is an axial view of a culture device for anaerobic ammonia-oxidizing bacteria of the present invention;

[0040] Figure 2 It is a schematic diagram of the fluid flow direction of the present invention;

[0041] Figure 3 is a top view of the reactor of the present invention;

[0042] Figure 4 yes Figure 3 Sectional view of the AA plane;

[0043] Figure 5 yes Figure 4 Enlarged view of point C in the middle;

[0044] Figure 6 yes Figure 4 The enlarged view of point D in the middle;

[0045] Figure 7 is a front view of the reactor of the present invention;

[0046] Figure 8 yes Figure 7 Sectional view of the middle BB plane;

[0047] Fig. 9 This is a schematic diagram of the connection between the first water distribution tray and the second water distribution tray. Figure 1 ;

[0048] Fig.10 This is a schematic diagram of the connection between the third water distribution plate, aeration plate and installation plate. Figure 1 ;

[0049] Fig.11 This is a schematic diagram of the connection between the third water distribution plate, aeration plate and installation plate. Figure 2 ;

[0050] Fig.12 This is a schematic diagram of the connection between the first water distribution tray and the second water distribution tray. Figure 2 .

[0051] In the figure:

[0052] 100-reactor, 101-discharge pipe, 110-carrier outer cylinder, 111-external temperature adjustment channel, 112-first sealing part, 120-carrier inner cylinder, 121-water flow channel, 122-inner temperature adjustment channel, 123-second sealing part, 130-biofilm, 140-first water distribution tray, 141-first water outlet hole, 142-connecting part, 143-first annular slit, 150-second water distribution tray, 151-second water outlet hole, 152-second annular slit, 160-third water distribution tray, 161-third water outlet hole, 170-aeration tray, 171-air outlet hole, 180-mounting tray, 181-through hole;

[0053] 200-cache box;

[0054] 300 - Temperature regulator. DETAILED DESCRIPTION

[0055] To make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be described in detail below. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other implementation methods obtained by ordinary technicians in this field without creative work belong to the scope of protection of the present invention.

[0056] The terms "first", "second", etc. in the specification and claims of the present application are used to distinguish similar objects, and are not used to describe a specific order or sequence. It should be understood that the data used in this way can be interchangeable under appropriate circumstances, so that the embodiments of the present application can be implemented in an order other than those illustrated or described here, and the objects distinguished by "first", "second", etc. are generally of one type, and the number of objects is not limited. For example, the first object can be one or more. In addition, "and / or" in the specification and claims represents at least one of the connected objects, and the character " / " generally indicates that the objects associated with each other are in an "or" relationship.

[0057] The applicant discovered during use that the existing device that relies on water to adjust the temperature of the culture device, such as the invention patent with application number CN202110171101.5, discloses a device for enriching and culturing anaerobic ammonia-oxidizing bacteria. During the heat exchange process, the temperature inside the culture device is adjusted at the periphery by relying on a temperature control device located outside the culture device. It takes a certain amount of time for the temperature to change from the outer biofilm 130 carrier to the inner biofilm 130 carrier, resulting in a delay in temperature regulation, and a temperature difference between the inside and outside of the biofilm 130 carrier during the regulation process, which affects the culture quality of the strain.

[0058] The following is combined with Figures 1 to 12 The culturing device and culturing method of the anaerobic ammonia-oxidizing bacteria provided in the present application are described in detail through specific embodiments and their application scenarios.

[0059] Some embodiments of the present application provide a culture device for anaerobic ammonia-oxidizing bacteria, such as Figure 1-Figure 12 , including a reactor 100, a carrier outer tube 110, a carrier inner tube 120, a temperature regulator 300, a buffer box 200, a first water distribution tray 140, a second water distribution tray 150, a third water distribution tray 160 and an aeration tray 170.

[0060] like Figure 2 and Figure 8 As shown, the carrier outer cylinder 110 is disposed in the reactor 100, and the outer wall of the carrier outer cylinder 110 and the inner wall of the reactor 100 form an outer temperature adjustment channel 111. The carrier inner cylinder 120 is disposed in the carrier outer cylinder 110, and the outer wall of the carrier inner cylinder 120 and the inner wall of the carrier outer cylinder 110 form a water flow channel 121. The carrier inner cylinder 120 also has an inner temperature adjustment channel 122 arranged along its own axial direction.

[0061] In the process of cultivating bacterial strains, sewage is introduced into the water flow channel 121 so that the sewage flows along the water flow channel 121. Heat exchange water flow is introduced into the external temperature adjustment channel 111 and the internal temperature adjustment channel 122 respectively so that the heat exchange water flow flows along the external temperature adjustment channel 111 and the internal temperature adjustment channel 122, and the sewage in the water flow channel 121 is heat exchanged, and the temperature in the water flow channel 121 is controlled at a temperature that is conducive to the reproduction of bacterial strains. In the process of heat exchange of sewage by the heat exchange water flow, the heat exchange water flow in the external temperature adjustment channel 111 exchanges heat with the sewage from the outside to the inside, and the heat exchange water flow in the internal temperature adjustment channel 122 exchanges heat with the sewage from the inside to the outside, thereby making the temperature adjustment response of the sewage fast and not prone to temperature difference.

[0062] like Figure 2 and Figure 4 As shown, the flow direction of water in the external temperature adjustment channel 111 and the internal temperature adjustment channel 122 is the same, which is opposite to the flow direction of water in the water flow channel 121 .

[0063] During the heat exchange process, water flows in the external temperature control channel 111 and the internal temperature control channel 122, and forms convection with sewage in the water flow channel 121 to enhance the heat transfer effect, so that heat can be transmitted faster, reduce the local temperature gradient, and thus be more conducive to the cultivation of bacteria.

[0064] It should be noted that, since the strains cultured in this embodiment are anaerobic ammonia oxidizing strains, as a common culture method for anaerobic ammonia oxidizing strains, water flows into the bottom of the reactor 100 to provide necessary nutrients for the anaerobic ammonia oxidizing bacteria, and then is discharged from the top of the reactor 100. Therefore, in this embodiment, the flow direction into the water flow channel 121 is set to flow from the bottom to the top, and the flow directions of the external temperature adjustment channel 111 and the internal temperature adjustment channel 122 are set to flow from the top to the bottom to meet the culture of anaerobic ammonia oxidizing strains.

[0065] like Figure 4 and Figure 8 As shown, the inner wall of the carrier outer cylinder 110 and the outer wall of the carrier inner cylinder 120 are respectively provided with biofilms 130. The water flow channel 121 formed by the inner wall of the carrier outer cylinder 110 and the outer wall of the carrier inner cylinder 120 is the main space for bacterial culture. Therefore, the biofilm 130 on the inner wall of the carrier outer cylinder 110 and the outer wall of the carrier inner cylinder 120 provides a stable growth environment for the bacterial species, so that the bacterial species can be firmly attached to the surface of the biofilm 130, thereby reducing the loss of bacterial species in the reactor 100 and maintaining the stability of the reactor 100.

[0066] like Figure 1 , Figure 2 and Figure 4As shown, the water outlet of the water flow channel 121 is connected to the water inlet of the cache box 200 , and the water outlet of the cache box 200 is connected to the water inlet of the external temperature adjustment channel 111 and the water inlet of the internal temperature adjustment channel 122 after passing through the temperature regulator 300 .

[0067] In order to achieve continuous heat exchange of sewage, the water exchange water needs to continuously flow from the top of the reactor 100 into the external temperature adjustment channel 111 and the internal temperature adjustment channel 122, and be discharged from the bottom of the reactor 100. In this process, a large amount of water is consumed. During the cultivation of the bacteria, the sewage continuously enters the water flow channel 121 from the bottom of the reactor 100 and is discharged from the top of the water flow channel 121. Therefore, the water outlet of the water flow channel 121 is connected to the water inlet of the cache box 200, and the sewage flowing out of the reactor 100 is stored in the cache box 200. The water outlet of the cache box 200 is connected to the water inlet of the external temperature control channel 111 and the water inlet of the internal temperature control channel 122 after passing through the temperature regulator 300. After the sewage in the cache box 200 is heated by the temperature regulator 300, it enters the external temperature control channel 111 and the internal temperature control channel 122 as a heat exchange water flow, exchanges heat with the sewage in the water flow channel 121, thereby reusing water resources and reducing water waste.

[0068] like Figure 1 , Figure 2 and Figure 4 As shown, the water outlet of the external temperature adjustment channel 111 and the water outlet of the internal temperature adjustment channel 122 are connected to the water inlet of the cache box 200. The heat exchange water flow flowing out of the external temperature adjustment channel 111 and the internal temperature adjustment channel 122 can enter the cache box 200, and after being heated by the temperature regulator 300, it can enter the external temperature adjustment channel 111 and the internal temperature adjustment channel 122 again as the heat exchange water flow to exchange heat with the sewage in the water flow channel 121, further reusing the water source to reduce the waste of water source.

[0069] In addition, since the hot water flow flowing out of the external temperature control channel 111 and the internal temperature control channel 122 and the sewage in the water flow channel 121 themselves have a certain amount of heat, they only need to be heated slightly by the temperature regulator 300 before they can be used as hot water flow again, which can significantly reduce energy consumption.

[0070] Accordingly, the cache box 200 has a pressure relief port. When there is too much sewage in the cache box 200, part of the sewage can be discharged through the pressure relief port, so that the sewage in the cache box 200 is always in a state of an appropriate liquid level.

[0071] It should be noted that the water outlet of the external temperature adjustment channel 111, the water outlet of the internal temperature adjustment channel 122 and the water outlet of the water flow channel 121 are all connected to the water inlet of the buffer box 200 through a pipe, and the water outlet of the buffer box 200 is connected to the temperature regulator 300, the water inlet of the external temperature adjustment channel 111 and the water inlet of the internal temperature adjustment channel 122 through a pipe. In addition, according to the length of the pipeline, a water pump can be reasonably set to provide power for the flow of water in the pipeline.

[0072] like Figure 4 As shown, the first water distribution tray 140 is located above the external temperature adjustment channel 111. The water outlet of the buffer box 200 is connected to the water inlet of the first water distribution tray 140 through a pipeline. Specifically, the end of the pipeline extends into the reactor 100 and is connected to the water inlet of the first water distribution tray 140.

[0073] In some embodiments, Figure 4 and Fig. 9 As shown, the first water distribution tray 140 has a plurality of first water outlet holes 141 arranged in an annular shape on one side facing the external temperature control channel 111. After the hot water flow in the buffer box 200 enters the first water distribution tray 140 through the pipeline, it is discharged from the first water outlet holes 141 into the external temperature control channel 111. Since the plurality of first water outlet holes 141 are arranged in an annular shape, the hot water flow discharged from the plurality of first water outlets can enter the external temperature control channel 111 uniformly along the circumference of the external temperature control channel 111, so that the water flow rate at each location around the external temperature control channel 111 is as uniform as possible, so as to better exchange heat with the sewage in the water flow channel 121.

[0074] It should be noted that the plurality of first water outlet holes 141 may be arranged in one circle, two circles, three circles or more, and may be flexibly arranged according to the size of the external temperature adjustment channel 111 , and this embodiment does not limit this.

[0075] like Figure 4 and Fig. 9 As shown, the projections of the plurality of first water outlet holes 141 along the axial direction of the reactor 100 are located in the external temperature adjustment channel 111. When the hot water flow is discharged from the plurality of first water outlet holes 141, the projections of the plurality of first water outlet holes 141 along the axial direction of the reactor 100 are located in the external temperature adjustment channel 111, which can ensure that the hot water flow discharged from the plurality of first water outlet holes 141 can completely enter the external temperature adjustment channel 111, thereby reducing the flow resistance of the hot water flow when entering the external temperature adjustment channel 111.

[0076] In this embodiment, the aperture of the first water outlet hole 141 gradually increases from the water inlet close to the first water distribution tray 140 to the water inlet away from the first water distribution tray 140, thereby improving the uniformity of the flow of the first water distribution tray 140 to the external temperature adjustment channel 111.

[0077] In some embodiments, Figure 4 and Fig.12 As shown, the first water distribution tray 140 has a first annular slit 143 on one side facing the external temperature adjustment channel 111. After the hot water flow in the buffer box 200 enters the first water distribution tray 140 through the pipeline, it is discharged from the first water outlet 141 into the external temperature adjustment channel 111. Since the first annular slit 143 is continuous and uninterrupted, the hot water flow discharged from the first annular slit 143 can enter the external temperature adjustment channel 111 uniformly along the circumference of the external temperature adjustment channel 111, so that the water flow rate at each location in the circumference of the external temperature adjustment channel 111 is as uniform as possible, so as to better exchange heat with the sewage in the water flow channel 121.

[0078] It should be noted that the number of the first annular slits 143 may be one, two, three or more, and may be flexibly set according to the size of the external temperature adjustment channel 111 , which is not limited in this embodiment.

[0079] like Figure 4 and Fig.12 As shown, the projection of the first annular slit 143 along the axial direction of the reactor 100 is located in the external temperature adjustment channel 111. When the hot water flow is discharged from the first annular slit 143, the projection of the first annular slit 143 along the axial direction of the reactor 100 is located in the external temperature adjustment channel 111, which can ensure that the hot water flow discharged from the first annular slit 143 can completely enter the external temperature adjustment channel 111, thereby reducing the flow resistance of the hot water flow when entering the external temperature adjustment channel 111.

[0080] As a preferred embodiment of the present invention, the gap of the first annular gap 143 gradually increases from the water inlet close to the first water distribution tray 140 to the water inlet away from the first water distribution tray 140, thereby improving the uniformity of the flow of the first water distribution tray 140 to the external temperature adjustment channel 111.

[0081] like Figure 4 As shown, the first water distribution tray 140 is arranged on the top of the carrier outer tube 110. In this way, when the hot water flow is discharged from the first water outlet holes 141, it can be ensured that the hot water flow will not fall on the top of the carrier outer tube 110 to cause splashing, so that the hot water flow can all enter the external temperature adjustment channel 111.

[0082] like Figure 4 and Figure 5 As shown, the outer wall of the first water distribution tray 140 is connected to the inner wall of the reactor 100, and the carrier outer cylinder 110 is also sleeved with a first sealing portion 112 near the bottom, and the outer wall of the first sealing portion 112 is connected to the inner wall of the reactor 100. A sealed space is formed between the first water distribution tray 140 and the first sealing portion 112, and the sealed space is the external temperature adjustment channel 111.

[0083] Due to the presence of the first sealing portion 112, the hot water flow discharged from the first water outlets 141 can only flow in the external temperature control channel 111 and will not enter the water flow channel 121, thereby not affecting the sewage entering the water flow channel 121, thereby ensuring the stability of the reactor 100.

[0084] As a preferred embodiment of the present invention, the first sealing portion 112 is disposed at the bottom of the carrier outer tube 110, and the bottom of the first sealing portion 112 is coplanar with the bottom of the carrier outer tube 110. This arrangement can maximize the length of the external temperature adjustment channel 111, thereby increasing the heat exchange area and enhancing the heat exchange effect on the water flow channel 121.

[0085] In this embodiment, the water outlet of the external temperature adjustment channel 111 is arranged at the bottom of the reactor 100 close to the external temperature adjustment channel 111 to discharge the exchange water flow from the external temperature adjustment channel 111 .

[0086] like Figure 4 and Fig. 9 As shown, the second water distribution tray 150 is located above the inner temperature adjustment channel 122. The water outlet of the buffer box 200 is connected to the water inlet of the second water distribution tray 150 through a pipeline. Specifically, the end of the pipeline extends into the reactor 100 and is connected to the water inlet of the second water distribution tray 150.

[0087] In this embodiment, if Fig. 9 and Fig.12 As shown, the second water distribution tray 150 is located inside the first water distribution tray 140 and is fixedly connected to the first water distribution tray 140 through the connection portion 142. Accordingly, the end of the pipeline passes through the second water distribution tray 150 and the connection portion 142 and is connected to the water inlet of the first water distribution tray 140 to complete the water supply to the second water distribution tray 150.

[0088] The first water distribution tray 140 is connected to the inner wall of the reactor 100 , and the connecting portion 142 connects the first water distribution tray 140 and the second water distribution tray 150 , providing a basis for the installation of the second water distribution tray 150 in the reactor 100 .

[0089] In this embodiment, the first water distribution tray 140 , the second water distribution tray 150 and the connecting portion 142 are processed by an integral molding method.

[0090] In some embodiments, Figure 4 and Fig. 9As shown, the second water distribution tray 150 has a plurality of second water outlet holes 151 arranged in an annular pattern on one side facing the inner temperature adjustment channel 122. After the heat exchange water flow in the buffer box 200 enters the second water distribution tray 150 through the pipeline, it is discharged from the second water outlet holes 151 into the inner temperature adjustment channel 122. Since the plurality of second water outlet holes 151 are arranged in an annular pattern, the heat exchange water flow discharged from the plurality of second water outlets can enter the inner temperature adjustment channel 122 uniformly along the circumference of the inner temperature adjustment channel 122, so that the water flow rate at each location around the inner temperature adjustment channel 122 is as uniform as possible, so as to better exchange heat with the sewage in the water flow channel 121.

[0091] It should be noted that the plurality of second water outlet holes 151 can be arranged in one circle, two circles, three circles or more, and can be flexibly arranged according to the size of the inner temperature adjustment channel 122 , and this embodiment does not limit this.

[0092] like Figure 4 and Fig. 9 As shown, the projections of the plurality of second water outlet holes 151 along the axial direction of the reactor 100 are located in the inner temperature adjustment channel 122. When the hot water flow is discharged from the plurality of second water outlet holes 151, the projections of the plurality of second water outlet holes 151 along the axial direction of the reactor 100 are located in the inner temperature adjustment channel 122, which ensures that the hot water flow discharged from the plurality of second water outlet holes 151 can completely enter the inner temperature adjustment channel 122, thereby reducing the flow resistance of the hot water flow when entering the inner temperature adjustment channel 122.

[0093] As a preferred embodiment of the present invention, the diameter of the second water outlet hole 151 gradually increases from the water inlet close to the second water distribution tray 150 to the water inlet away from the second water distribution tray 150, thereby improving the uniformity of the flow of the second water distribution tray 150 to the internal temperature adjustment channel 122.

[0094] In some embodiments, Figure 4 and Fig.12 As shown, the second water distribution tray 150 has a second annular slit 152 on one side facing the inner temperature adjustment channel 122. After the hot water flow in the buffer box 200 enters the second water distribution tray 150 through the pipeline, it is discharged from the second water outlet 151 into the inner temperature adjustment channel 122. Since the second annular slit 152 is continuous and uninterrupted, the hot water flow discharged from the second annular slit 152 can enter the inner temperature adjustment channel 122 uniformly along the circumference of the inner temperature adjustment channel 122, so that the water flow rate at each location in the circumference of the inner temperature adjustment channel 122 is as uniform as possible, so as to better exchange heat with the sewage in the water flow channel 121.

[0095] It should be noted that the number of the second annular slits 152 may be one, two, three or more, and may be flexibly set according to the size of the inner temperature adjustment channel 122 , which is not limited in this embodiment.

[0096] like Figure 4 and Fig.12 As shown, the projection of the second annular slit 152 along the axial direction of the reactor 100 is located in the inner temperature adjustment channel 122. When the hot water flow is discharged from the second annular slit 152, the projection of the second annular slit 152 along the axial direction of the reactor 100 is located in the inner temperature adjustment channel 122, which can ensure that the hot water flow discharged from the second annular slit 152 can completely enter the inner temperature adjustment channel 122, thereby reducing the flow resistance of the hot water flow when entering the inner temperature adjustment channel 122.

[0097] In this embodiment, the gap of the second annular gap 152 gradually increases from the water inlet close to the second water distribution tray 150 to the water inlet away from the second water distribution tray 150, thereby improving the uniformity of the flow of the second water distribution tray 150 to the internal temperature adjustment channel 122.

[0098] like Figure 4 As shown, the second water distribution tray 150 is arranged on the top of the carrier inner tube 120. In this way, when the hot water flow is discharged from the second water outlet holes 151, it can be ensured that the hot water flow will not fall on the top of the carrier inner tube 120 to cause splashing, so that the hot water flow can all enter the inner temperature adjustment channel 122.

[0099] like Figure 4 and Figure 5 As shown, the second water distribution tray 150 covers the inner temperature adjustment channel 122, and a second sealing portion 123 is provided near the bottom of the inner temperature adjustment channel 122, and the outer wall of the second sealing portion 123 is connected to the inner wall of the carrier inner cylinder 120. A sealed space is formed between the second water distribution tray 150 and the second sealing portion 123, and the sealed space is the inner temperature adjustment channel 122.

[0100] Due to the existence of the second sealing portion 123, the heat exchange water flow discharged from the plurality of second water outlet holes 151 can only flow in the internal temperature adjustment channel 122 and will not enter the water flow channel 121, thereby not affecting the sewage entering the water flow channel 121, thereby ensuring the stability of the reactor 100.

[0101] As a preferred embodiment of the present invention, the second sealing portion 123 is disposed at the bottom of the carrier inner tube 120, and the bottom of the second sealing portion 123 is coplanar with the bottom of the carrier inner tube 120. This arrangement can maximize the length of the inner temperature adjustment channel 122, thereby increasing the heat exchange area and enhancing the heat exchange effect on the water flow channel 121.

[0102] In this embodiment, the water outlet of the internal temperature adjustment channel 122 is arranged at the bottom of the second sealing part 123, extends into the reactor 100 through a pipeline, and is connected to the water outlet arranged in the second sealing part 123 to discharge the exchange water flow from the internal temperature adjustment channel 122.

[0103] like Figure 4 and Figure 6 As shown, a third water distribution tray 160 is provided below the water flow channel 121. The pipeline extends into the reactor 100 and is connected to the water inlet of the third water distribution tray 160, so that external sewage can enter the third water distribution tray 160 to complete the water supply to the third water distribution tray 160.

[0104] like Figure 4 , Figure 6 , Fig.10 and Fig.11 As shown, the third water distribution tray 160 has a plurality of third water outlet holes 161 arranged in an annular shape on one side facing the water flow channel 121. After the external sewage enters the third water distribution tray 160 through the pipe, it is discharged from the third water outlet holes 161 into the water flow channel 121. Since the plurality of third water outlet holes 161 are arranged in an annular shape, the sewage discharged from the plurality of third water outlet holes can enter the water flow channel 121 uniformly along the circumference of the water flow channel 121, so that the water flow rate at each location in the circumference of the water flow channel 121 is as uniform as possible, so as to better exchange heat between the sewage in the water flow channel 121 and the heat exchange water flow of the internal temperature control channel 122 and the heat exchange water flow of the external and internal parts.

[0105] It should be noted that the plurality of third water outlet holes 161 can be arranged in one circle, two circles, three circles or more, and can be flexibly arranged according to the size of the water flow channel 121 , and this embodiment does not limit this.

[0106] like Figure 4 As shown, the projections of the plurality of third water outlet holes 161 along the axial direction of the reactor 100 are located in the water flow channel 121. When sewage is discharged from the plurality of third water outlet holes 161, the projections of the plurality of third water outlet holes 161 along the axial direction of the reactor 100 are located in the water flow channel 121, which can ensure that the sewage discharged from the plurality of third water outlet holes 161 can completely enter the water flow channel 121, thereby reducing the flow resistance of the sewage when entering the water flow channel 121.

[0107] like Figure 4 , Figure 6 , Fig.10 and Fig.11 As shown, the aeration plate 170 is located below the water flow channel 121. The pipeline extends into the reactor 100 and is connected to the air inlet of the aeration plate 170, so that external nitrogen can enter the aeration plate 170 to complete the air supply to the aeration plate 170. Nitrogen is an inert gas that is not easy to react chemically with other substances and has a very low solubility in water. Therefore, the introduction of nitrogen can effectively replace the oxygen in the reactor 100, creating an anaerobic or hypoxic environment, which is conducive to the cultivation of bacterial strains.

[0108] like Figure 4 and Fig.11As shown, the aeration plate 170 has a plurality of annularly arranged air outlets 171 on one side facing the water flow channel 121. After the external nitrogen enters the aeration plate 170 through the pipeline, it is discharged from the air outlets 171 into the water flow channel 121. Since the air outlets 171 are arranged in an annular shape, the nitrogen discharged from the plurality of air outlets 171 can enter the water flow channel 121 uniformly along the circumference of the water flow channel 121, so that the nitrogen flow rate at each location around the water flow channel 121 is as uniform as possible, so as to better replace the oxygen in the reactor 100 and better create an oxygen-free or low-oxygen environment.

[0109] It should be noted that the plurality of air outlet holes 171 can be arranged in one circle, two circles, three circles or more, and can be flexibly arranged according to the size of the water flow channel 121 , and this embodiment does not limit this.

[0110] like Figure 4 As shown, the projections of the plurality of gas outlet holes 171 along the axial direction of the reactor 100 are located in the water flow channel 121. When nitrogen is discharged from the plurality of gas outlet holes 171, the projections of the plurality of gas outlet holes 171 along the axial direction of the reactor 100 are located in the water flow channel 121, which can ensure that the nitrogen discharged from the plurality of gas outlet holes 171 can completely enter the water flow channel 121, thereby reducing the flow resistance of the nitrogen when entering the water flow channel 121.

[0111] like Figure 4 , Figure 6 , Fig.10 and Fig.11 As shown, the reactor 100 further includes a mounting plate 180, and a plurality of third water outlet holes 161 and a plurality of air outlet holes 171 pass through the mounting plate 180 and are arranged alternately. The alternating arrangement of the plurality of third water outlet holes 161 and the plurality of air outlet holes 171 further ensures the stability and uniformity of the anaerobic environment in the reactor 100, thereby avoiding the inhibitory effect of oxygen on the strains, which is beneficial to the cultivation of the strains.

[0112] Specifically, Figure 4 and Figure 6 As shown, the outer wall of the mounting plate 180 is connected to the inner wall of the reactor 100 , and the mounting plate 180 provides an installation base for the third water distribution plate 160 and the aeration plate 170 , so that the third water distribution plate 160 and the aeration plate 170 can be stably installed in the reactor 100 .

[0113] like Figure 1 and Figure 4As shown, a discharge pipe 101 is provided at the bottom of the reactor 100. Through the discharge pipe 101 at the bottom, the sludge in the reactor 100 can be discharged regularly to achieve the renewal of the sludge, thereby maintaining the cleanliness and efficient operation of the reactor 100. Accordingly, in order to facilitate the discharge of the sludge in the reactor 100 through the discharge pipe 101, the mounting plate 180 has a through hole 181 penetrating along its own axial direction, and the third water distribution plate 160 and the aeration plate 170 are made of annularly wound pipes, so that the sludge can be discharged from the reactor 100 through the mounting plate 180, the third water distribution plate 160 and the aeration plate 170.

[0114] Some embodiments of the present application also provide a method for culturing anaerobic ammonia-oxidizing bacteria, which is applied to a device for culturing anaerobic ammonia-oxidizing bacteria, and comprises the following steps:

[0115] Step 100: introducing sewage into the water flow channel 121 so that the sewage flows along the water flow channel 121;

[0116] Step 200: introducing a hot water flow into the external temperature adjustment channel 111 and the internal temperature adjustment channel 122 respectively, so that the hot water flow flows along the external temperature adjustment channel 111 and the internal temperature adjustment channel 122 and is opposite to the flow direction of the sewage in the water flow channel 121;

[0117] Step 300: The water exchange water flow in the external temperature control channel 111 and the water exchange water flow in the internal temperature control channel 122 perform heat exchange on the sewage in the water flow channel 121, so that the bacteria are cultured in the water flow channel 121. It should be noted that, in this article, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device. In the absence of further restrictions, an element defined by the sentence "includes a..." does not exclude the existence of other identical elements in the process, method, article or device including the element.

[0118] In addition, it should be noted that the scope of the methods and devices in the embodiments of the present application is not limited to performing functions in the order shown or discussed, and may also include performing functions in a substantially simultaneous manner or in a reverse order according to the functions involved. For example, the described methods may be performed in an order different from that described, and various steps may be added, omitted, or combined. In addition, features described with reference to certain examples may be combined in other examples.

[0119] The above description is only a specific implementation mode of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can easily think of changes or substitutions within the technical scope disclosed by the present invention, which should be covered by the protection scope of the present invention.

Claims

1. A culture device for anaerobic ammonium-oxidizing bacteria, characterized in that: include: The carrier outer cylinder is arranged in the reactor; the outer wall of the carrier outer cylinder and the inner wall of the reactor form an external temperature adjustment channel; The carrier inner cylinder is arranged in the carrier outer cylinder; the outer wall of the carrier inner cylinder and the inner wall of the carrier outer cylinder form a water flow channel; the carrier inner cylinder also has an inner temperature adjustment channel arranged along its own axial direction; A cache box, the water inlet of the cache box is respectively connected to the water outlet of the external temperature adjustment channel, the water outlet of the internal temperature adjustment channel and the water outlet of the water flow channel; the water outlet of the cache box is connected to the water inlet of the external temperature adjustment channel and the water inlet of the internal temperature adjustment channel after passing through a temperature regulator; Wherein, the external temperature adjustment channel, the internal temperature adjustment channel and the water flow channel are configured such that: the flow direction of the water in the external temperature adjustment channel and the internal temperature adjustment channel is the same, and opposite to the flow direction of the water in the water flow channel; The inner wall of the carrier outer cylinder and the outer wall of the carrier inner cylinder are respectively provided with biofilms for attaching bacteria.

2. The anaerobic ammonium-oxidizing bacteria culture device according to claim 1, characterized in that: It also includes a first water distribution pan, which is located above the external temperature adjustment channel; The first water distribution tray has a plurality of first water outlet holes arranged in an annular shape on one side facing the external temperature adjustment channel, and projections of the plurality of first water outlet holes along the axial direction of the reactor are located in the external temperature adjustment channel; Alternatively, a first annular slit is provided on a side of the first water distribution tray facing the external temperature adjustment channel, and a projection of the first annular slit along the axial direction of the reactor is located within the external temperature adjustment channel.

3. The anaerobic ammonium-oxidizing bacteria culture device according to claim 2, characterized in that: The apertures of the first water outlet holes gradually increase from the water inlet close to the first water distribution tray to the water inlet far away from the first water distribution tray; Alternatively, the gap of the first annular gap gradually increases from a direction close to the water inlet of the first water distribution tray to a direction away from the water inlet of the first water distribution tray.

4. The anaerobic ammonium-oxidizing bacteria culture device according to claim 2, characterized in that: The first water distribution tray is arranged on the top of the carrier outer cylinder, and its outer wall is connected to the inner wall of the reactor; A first sealing portion is also sleeved on a position of the carrier outer cylinder near the bottom, and an outer wall of the first sealing portion is connected to an inner wall of the reactor; Wherein, the external temperature adjustment channel is the space between the first sealing part and the first water distribution tray.

5. The anaerobic ammonium-oxidizing bacteria culture device according to claim 1, characterized in that: It also includes a second water distribution pan, which is located above the inner temperature adjustment channel; The second water distribution tray has a plurality of second water outlet holes arranged in an annular shape on one side facing the inner temperature adjustment channel, and the axial projections of the plurality of second water outlet holes on the reactor are located in the inner temperature adjustment channel; Alternatively, a second annular slit is provided on a side of the second water distribution tray facing the inner temperature adjustment channel, and a projection of the second annular slit along the axial direction of the reactor is located within the inner temperature adjustment channel.

6. The anaerobic ammonium-oxidizing bacteria culture device according to claim 5, characterized in that: The apertures of the plurality of second water outlet holes gradually increase from the water inlet close to the second water distribution tray to the water inlet far away from the second water distribution tray; Alternatively, the gap of the second annular gap gradually increases from a direction close to the water inlet of the second water distribution tray to a direction away from the water inlet of the second water distribution tray.

7. The anaerobic ammonium-oxidizing bacteria culture device according to claim 5, characterized in that: The second water distribution tray is arranged on the top of the carrier inner cylinder and covers the inner temperature adjustment channel; A second sealing portion is provided near the bottom of the inner temperature adjustment channel, and an outer wall of the second sealing portion is connected to an inner wall of the carrier inner cylinder; Wherein, the inner temperature adjustment channel is the space between the second sealing portion and the second water distribution tray.

8. The anaerobic ammonium-oxidizing bacteria culture device according to claim 1, characterized in that: It also includes a third water distribution tray, and the third water distribution tray is located below the water flow channel; The third water distribution tray has a plurality of third water outlet holes arranged in a ring shape on one side facing the water flow channel, and projections of the plurality of third water outlet holes along the axial direction of the reactor are located in the water flow channel.

9. The anaerobic ammonium-oxidizing bacteria culture device according to claim 8, characterized in that: Also includes an aeration plate, wherein the aeration plate is located below the water flow channel; The aeration plate has a plurality of annularly arranged air outlet holes on one side facing the water flow channel, and the projections of the plurality of air outlet holes along the axial direction of the reactor are located in the water flow channel; And / or, it also includes a mounting plate, and the plurality of the third water outlet holes and the plurality of the air outlet holes respectively pass through the mounting plate and are alternately arranged.

10. A method for cultivating anaerobic ammonia-oxidizing bacteria, applied to the cultivation device according to any one of claims 1 to 9, characterized in that: The following steps are involved: Introducing sewage into the water flow channel so that the sewage flows along the water flow channel; The hot water exchange flow is introduced into the external temperature adjustment channel and the internal temperature adjustment channel respectively, so that the hot water exchange flow flows along the external temperature adjustment channel and the internal temperature adjustment channel and is opposite to the flow direction of the sewage in the water flow channel; The hot water exchange flow in the external temperature regulating channel and the hot water exchange flow in the internal temperature regulating channel perform heat exchange on the sewage in the water flow channel, so that the bacteria are cultured in the water flow channel.

Images