Culture device and culture method of photosynthetic bacteria and photosynthetic bacteria liquid

Abstract

The invention provides a culture device and a culture method of a photosynthetic bacterium liquid and the photosynthetic bacterium liquid. The culture device comprises a culture box, a containing cavity, a plurality of first LED plane light source plates, a plurality of second LED plane light source plates and a submersible plug flow pump, the accommodating cavity is vertically inserted in the middle of the bottom of the culture box, the width of the accommodating cavity is smaller than that of the culture box, and the culture box and the accommodating cavity are made of transparent materials; the first LED plane light source plates are arranged in the containing cavity and distributed in two rows, and light sources face outwards. The second LED plane light source plates are uniformly distributed on the outer side of the bottom of the incubator, and light sources face upwards; and the submersible plug flow pump is arranged in the culture box. The culture method comprises the following steps: preparing a photosynthetic bacterium liquid culture medium, inoculating a bacterium seed liquid, carrying out illumination culture by utilizing an incubator and the like. The photosynthetic bacteria liquid is prepared by adopting the culture method. According to the invention, illumination balance can be ensured, so that the photosynthetic bacteria can reach a high growth rate, and the production efficiency of the photosynthetic bacteria liquid is further improved.

Description

Technical Field

[0001] The present application relates to the field of photosynthetic bacteria culture, and in particular to a photosynthetic bacteria culture device, a culture method and photosynthetic bacteria culture. Background Art

[0002] Photosynthetic bacteria (PSB) can undergo photosynthetic phosphorylation and related reactions in an anaerobic, light-irradiated environment, decompose organic matter, and grow in a photo-heterotrophic manner. In an aerobic environment, photosynthetic bacteria can use organic matter to carry out the tricarboxylic acid cycle. This characteristic of changing metabolic types according to the environment and having strong adaptability makes photosynthetic bacteria an excellent biological material for treating organic wastewater. Photosynthetic bacteria can effectively remove organic matter, as well as nitrogen, phosphorus, and sulfide in wastewater. They are often used in in-situ treatment of river water bodies and aquaculture to reduce ammonia nitrogen, nitrite, and organic pollutants in water bodies.

[0003] In the existing photosynthetic bacteria culture device, since the photosynthetic bacteria liquid in various places cannot be irradiated by sufficient and balanced light sources, the growth rate of the photosynthetic bacteria in the culture device is low and the production efficiency is very low.

[0004] There is no culture device in the prior art that can ensure that photosynthetic bacterial liquid at each location is irradiated with sufficient and balanced light source, so the prior art needs to be improved. Summary of the invention

[0005] The purpose of the present invention is to provide a photosynthetic bacteria culture liquid culture device, a culture method and photosynthetic bacteria culture liquid, which can solve the problem that the existing culture device cannot ensure that the photosynthetic bacteria liquid at various locations is irradiated with a balanced and sufficient light source, and can improve the growth rate of the photosynthetic bacteria in the culture liquid.

[0006] The present invention provides a photosynthetic bacteria culture device, comprising a culture box, a containing cavity, a plurality of first LED plane light source boards, a plurality of second LED plane light source boards and a submersible flow pump; the containing cavity is arranged in the culture box, and the containing cavity is vertically inserted in the middle position of the bottom of the culture box, the width of the containing cavity is smaller than the width of the culture box, and the culture box and the containing cavity are both made of transparent materials; a plurality of the first LED plane light source boards are arranged in the containing cavity, and the plurality of the first LED plane light source boards are distributed in two rows, and the light source of each first LED plane light source board faces outward; a plurality of the second LED plane light source boards are evenly distributed on the outer side of the bottom of the culture box, and the light source of each second LED plane light source board faces upward; the submersible flow pump is arranged in the culture box, and is used for pushing the photosynthetic bacteria liquid to form a circulation reflux.

[0007] Preferably, in the culture device for photosynthetic bacterial liquid of the present invention, the first LED planar light source board and the second LED planar light source board both include a heat dissipation shell, an LED COB light board and a light homogenizing plate, the heat dissipation shell has an opening on one side, the LED COB light board is arranged in the heat dissipation shell, and its light source is facing the opening of the heat dissipation shell, and the light homogenizing plate seals the opening of the heat dissipation shell.

[0008] Preferably, in the photosynthetic bacteria liquid culture device of the present invention, both the first LED planar light source panel and the second LED planar light source panel are LED light sources that emit a mixture of blue light and yellow light.

[0009] Preferably, the photosynthetic bacteria liquid culture device of the present invention further comprises a heating tube, and the heating tube is vertically arranged in the culture box.

[0010] Preferably, the photosynthetic bacteria liquid culture device of the present invention further comprises a pH detector, and the pH detector is arranged in the culture box.

[0011] Preferably, the photosynthetic bacteria liquid culture device of the present invention further includes a PLC control system, and the PLC control system is connected to the first LED planar light source board, the second LED planar light source board, the submersible flow pump, the heating tube and the pH detector.

[0012] The present invention also provides a method for culturing a photosynthetic bacterial liquid, comprising the following steps: A. Weigh 20 parts of sodium acetate, 1 part of sodium glutamate, 10 parts of ammonium chloride, 4 parts of magnesium sulfate, 5 parts of potassium dihydrogen phosphate, 10 parts of sodium chloride, 10 parts of yeast extract powder and 10,000 parts of water according to weight ratio, and place them in an incubator to obtain a culture medium of photosynthetic bacterial liquid; B. Inoculate the seed solution of Rhodopseudomonas palustris and Rhodospirilla crimsonii into the incubator at a ratio of 1:1, with the inoculation amount of 10% to 12% for both. 600 ≥1.860; C. Place the incubator in an open, sunny place outdoors. At night or when there is no sunlight outdoors, turn on the first LED flat light source panel in the incubator and the second LED flat light source panel on the outside of the bottom of the incubator. Cultivate according to the preset cultivation cycle. When the OD value of the photosynthetic bacterial solution in the incubator reaches 660 When the pH value is greater than 1.5, photosynthetic bacterial solution is obtained.

[0013] Preferably, in the method for culturing photosynthetic bacteria liquid of the present invention, the following step is further included between step B and step C: measuring the pH value of the culture medium of the photosynthetic bacteria liquid by a pH detector in the incubator, and adding sodium hydroxide solution to adjust the pH value to 7.5.

[0014] Preferably, in the method for culturing photosynthetic bacterial liquid of the present invention, in step C, the heating tube in the incubator is controlled by a PLC control system, the temperature in the incubator is set to be constant at 28°C to 36°C, and the start time and stop time of the first LED planar light source panel and the second LED planar light source panel are set.

[0015] The invention also provides a photosynthetic bacteria liquid, which is prepared by adopting the photosynthetic bacteria liquid culture method.

[0016] The beneficial effects of the present application are as follows: the present invention provides a accommodating cavity made of transparent material in the middle position of a transparent incubator, a double-row LED flat light source board is arranged inside the accommodating cavity, and a plurality of LED flat light source boards are evenly arranged at the bottom of the incubator, so that the photosynthetic bacterial liquid in the incubator can obtain sufficient and balanced light in both the longitudinal and transverse directions, and a submersible plug flow pump is also provided in the incubator. Under the plug flow of the submersible plug flow pump, the photosynthetic bacterial liquid in the incubator forms a circulating reflux, and combined with the LED flat light sources in the middle position and the bottom of the incubator, the photosynthetic bacterial liquid in various places can obtain balanced light source irradiation, thereby improving the photosynthetic bacteria to achieve a high growth rate; the LED flat light source board is used as the lighting setting for the cultivation of photosynthetic bacterial liquid, so as to further ensure the balance of light, so as to achieve the effect of efficient production of photosynthetic bacterial liquid. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the technical solution of the present invention, the drawings required for use in the present invention will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present application and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without paying creative work.

[0018] Figure 1 It is a structural schematic diagram of a photosynthetic bacteria liquid culture device from a first perspective of the present invention; Figure 2 It is a structural schematic diagram of a photosynthetic bacteria liquid culture device from a second viewing angle of the present invention; Figure 3 It is a top view without a cover of a photosynthetic bacteria liquid culture device of the present invention; Figure 4 It is a schematic structural diagram of a photosynthetic bacteria liquid culture device from a third perspective of the present invention; Figure 5 It is a schematic flow chart of a method for culturing photosynthetic bacteria liquid of the present invention; Figure 6 It is a comparison table of technical effects of the present invention.

[0019] Notes in the figure: 10, incubator; 11, cover plate; 20, accommodating cavity; 30, first LED flat light source board; 40, second LED flat light source board; 50, submersible flow pump; 60, heating tube. DETAILED DESCRIPTION

[0020] A specific embodiment will be provided below in conjunction with the accompanying drawings of the present invention to clearly and completely describe the technical solution of the present invention.

[0021] In the description of the embodiments of the present application, it should be noted that the terms "inside", "outside", "internal", "external", etc. indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings, or the orientation or positional relationship in which the product of the application is usually placed when in use. They are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present application. In addition, the terms "first", "second", etc. are only used to distinguish the description, and cannot be understood as indicating or implying relative importance. For those of ordinary skill in the art, the specific meanings of the above terms in the present application can be understood according to specific circumstances.

[0022] like Figure 1-4 As shown, an embodiment of the present application provides a culture device for photosynthetic bacterial liquid, including an incubator 10, a containing cavity 20, a plurality of first LED flat light source panels 30, a plurality of second LED flat light source panels 40 and a submersible flow pump 50, wherein the incubator 10 is also provided with a cover plate 11. Among them, the containing cavity 20 is arranged in the incubator 10, and is vertically inserted in the middle position of the bottom of the incubator 10, but the width of the containing cavity 20 is smaller than the width of the incubator 10, so that the liquid outside the two ends of the containing cavity 20 can circulate. And the incubator 10 and the containing cavity 20 are both made of transparent materials so as to be light-transmissive. In practical applications, the transparent material is transparent organic glass, and the incubator 10 is a cuboid, which is 1.5 meters long, 1 meter high and 1 meter wide.

[0023] Furthermore, a plurality of first LED planar light source panels 30 are arranged in the accommodating cavity 20, and the plurality of first LED planar light source panels 30 are distributed in two rows, and the light source of each first LED planar light source panel 30 faces outward, providing sufficient light source for photosynthetic bacteria liquid in the horizontal direction; and a plurality of second LED planar light source panels 40 are evenly distributed on the outside of the bottom of the incubator 10, and the light source of each second LED planar light source panel 40 faces upward, providing sufficient light source for photosynthetic bacteria liquid in the vertical direction, and the first LED planar light source panel 30 and the second LED planar light source panel 40 jointly provide sufficient light source for the liquid in the incubator 10. The submersible flow pump 50 is arranged in the incubator 10, and is used to push the photosynthetic bacteria liquid to form a circulation reflux, so that the liquid in each place is evenly illuminated.

[0024] In the photosynthetic bacteria culture device of the embodiment of the present application, the first LED planar light source board 30 and the second LED planar light source board 40 both include a heat dissipation housing, an LED COB light board and a light homogenizing plate. Among them, the heat dissipation housing has an opening on one side and can be made of a material with a high thermal conductivity such as aluminum, which can quickly dissipate the heat generated by the LED planar light source board, and the LED COB light board is arranged in the heat dissipation housing, and its light source faces the opening of the heat dissipation housing, which can provide uniform illumination, and the light homogenizing plate seals the opening of the heat dissipation housing, further improving the light uniformity of the LED planar light source board.

[0025] It should be noted that the existing photosynthetic bacteria culture device generally sets an LED point light source above the device, and the cultured bacteria liquid inside the device can only be illuminated in the vertical direction, the illumination is insufficient and unbalanced, and the light emitted by the LED point light source is also very scattered, so that the cultured bacteria liquid in various places in the device cannot be illuminated evenly, and the growth rate of some photosynthetic bacteria is slow, and the production efficiency is low. In addition, the existing photosynthetic bacteria culture device can only be cultured under one illumination condition, and it is impossible for a culture device to realize the selection of natural light or LED light source. The culture device of the embodiment of the present application is provided with an LED plane light source at the bottom and the middle position of the interior of the incubator 10, which can provide sufficient LED light sources for the bacteria liquid in the horizontal and vertical directions, and combined with the submersible flow pump 40, a highly balanced illumination can be achieved, so that as many photosynthetic bacteria as possible in the incubator 10 can reach a high growth rate. And there is no need to use an expensive waterproof LED light source. The LED plane light source board of the embodiment of the present application is isolated from the bacteria liquid and does not directly contact it, which can also avoid the heat generated by the LED plane light source from affecting the temperature in the incubator 10 to the greatest extent, thereby affecting the growth rate of photosynthetic bacteria. Since the incubator 10 is made of a transparent material, the embodiment of the present application can use natural light for culture during the day. Natural light irradiates the bacterial liquid in the incubator 10 from the outside and the top of the incubator 10, which saves energy. At night, the LED flat light source irradiates the bacterial liquid in the incubator 10 from the middle and the bottom of the incubator 10, so that a variety of lighting conditions can be selected for one device.

[0026] In the photosynthetic bacteria culture device of the embodiment of the present application, the first LED plane light source board 30 and the second LED plane light source board 40 are both LED light sources that emit a mixture of blue light and yellow light. The LED blue light beads and the LED yellow light beads on the light source board are evenly adjacent and spaced in the vertical and horizontal directions, and can provide the optimal lighting conditions for Rhodopseudomonas palustris and Rhodospirilla crimson at the same time. In practical applications, the yellow light wavelength of the first LED plane light source board 30 and the second LED plane light source board is 550nm, the blue light wavelength is 400nm, and the power is 18W.

[0027] Furthermore, the photosynthetic bacteria culture device of the embodiment of the present application further includes a heating tube 60 , which is vertically disposed in the incubator 10 and is used to control the temperature in the incubator 10 to provide the photosynthetic bacteria with an optimal temperature condition.

[0028] In addition, the culturing device for photosynthetic bacteria liquid of the embodiment of the present application also includes a pH detector (not shown in the figure), which is arranged in the incubator 10 and is used to detect the pH value of the liquid in the incubator 10 of the bacterial liquid to provide optimal pH conditions for the photosynthetic bacteria.

[0029] In practical applications, the photosynthetic bacteria culture device of the embodiment of the present application further includes a PLC control system, which is arranged outside the incubator (not shown in the figure), and the PLC control system is connected to the first LED flat light source board 30, the second LED flat light source board 40, the submersible flow pump 50, the heating tube 60 and the pH detector, and the connecting wires entering the incubator enter through the holes on the cover plate 11. The PLC control system is programmed to set the operation control of the first LED flat light source board 30, the second LED flat light source board 40, the submersible flow pump 50 and the heating tube 60, so that the photosynthetic bacteria in the incubator 10 can reach a high growth rate.

[0030] like Figure 5 As shown, the present application embodiment also provides a method for culturing a photosynthetic bacterial liquid, comprising the following steps: A. Weigh 20 parts of sodium acetate, 1 part of sodium glutamate, 10 parts of ammonium chloride, 4 parts of magnesium sulfate, 5 parts of potassium dihydrogen phosphate, 10 parts of sodium chloride, 10 parts of yeast extract powder and 10,000 parts of water according to weight ratio, and place them in an incubator to obtain a culture medium of photosynthetic bacterial liquid; B. Inoculate the seed solution of Rhodopseudomonas palustris and Rhodospirilla crimsonii into the incubator at a ratio of 1:1, with the inoculation amount of 10% to 12% for both. The OD of the seed solution 600 ≥1.860; C. Place the incubator in an open, sunny place outdoors and / or turn on the first LED flat light source panel in the incubator and the second LED flat light source panel outside the incubator. Cultivate according to the preset cultivation cycle. When the OD of the photosynthetic bacterial solution in the incubator reaches 660 When the pH value is greater than 1.5, photosynthetic bacterial solution is obtained.

[0031] It should be noted that the photosynthetic bacteria culture of the embodiment of the present application uses sodium acetate as a carbon source, and the proportion of sodium acetate used after the culture medium in the device is inoculated with bacteria is about 2g / L. The photosynthetic bacteria culture of the embodiment of the present application uses yeast extract powder as a quick-acting nitrogen source and a rapid growth factor to stimulate the rapid growth of photosynthetic bacteria. The proportion of yeast extract powder used after the culture medium in the device is inoculated with bacteria is 0.5g / L to 1g / L.

[0032] It should be further explained that during the cultivation of the photosynthetic bacterial liquid of the present application, one tenth of the space is left in the incubator.

[0033] In practical applications, the culture cycle of the bacterial solution in the incubator is based on the culture temperature and light conditions. 660 When the pH value is greater than 1.5, the obtained photosynthetic bacterial solution is packaged in small cans for delivery.

[0034] In the method for culturing photosynthetic bacteria liquid of the embodiment of the present application, the following steps are also included between step B and step C: measuring the pH value of the culture medium of the photosynthetic bacteria liquid by a pH detector in the incubator, and adding sodium hydroxide solution to adjust the pH value to 7.5.

[0035] In the culturing method of photosynthetic bacterial liquid of the embodiment of the present application, in step C, the heating tube in the incubator is controlled by the PLC control system, the temperature in the incubator is set to be constant at 28°C to 36°C, and the on time and off time of the first LED planar light source panel and the second LED planar light source panel are set, so as to ensure that the temperature, light and pH value in the incubator are the optimal conditions for the growth of photosynthetic bacteria, thereby improving the production rate.

[0036] The embodiment of the present application also provides a photosynthetic bacteria liquid, which is prepared by the culturing method of the photosynthetic bacteria liquid.

[0037] Compared with the photosynthetic bacteria culture device and method of the prior art, the embodiment of the present application improves the growth rate of photosynthetic bacteria and shortens the production cycle of photosynthetic bacteria liquid. The technical effect comparison table is attached. Figure 6 .

[0038] It should be noted that, in the embodiment of the present application, a accommodating cavity made of a transparent material is provided in the middle position of the transparent incubator, a double row of LED flat light source panels are arranged inside the accommodating cavity, and a plurality of LED flat light source panels are evenly arranged at the bottom of the incubator, so that the photosynthetic bacterial liquid in the incubator can obtain sufficient and balanced light in both the longitudinal and transverse directions, and a submersible plug flow pump is also provided in the incubator. Under the plug flow of the submersible plug flow pump, the photosynthetic bacterial liquid in the incubator forms a circulating reflux, and combined with the LED flat light sources in the middle position and the bottom of the incubator, the photosynthetic bacterial liquid in various places can be evenly irradiated by the light source, thereby improving the photosynthetic bacteria to achieve a high growth rate; the LED flat light source panel is used as the lighting setting for the cultivation of photosynthetic bacterial liquid, further ensuring the balance of the lighting, so as to achieve the effect of efficient production of photosynthetic bacterial liquid.

[0039] The embodiments of the present application optimize the culture medium and the culture light source for the culture process of photosynthetic bacteria, thereby increasing the growth rate of the bacteria and the utilization of the substrate; improve the extensive culture process of the photosynthetic bacteria Rhodopseudomonas palustris and Rhodospirilla rubrum, thereby realizing temperature control, light supply control, bacterial liquid flow control, and pH control during the culture process; increase the service life of the electric light source and reduce the consumption of electrical energy; and are suitable for large-scale production of the photosynthetic bacteria Rhodopseudomonas palustris and Rhodospirilla rubrum.

[0040] The above description is only an embodiment of the present application and is not intended to limit the scope of protection of the present application. For those skilled in the art, the present application may have various modifications and changes. Unless otherwise clearly specified and limited, the terms "setting" and "connection" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be directly connected, or indirectly connected through an intermediate medium, or it can be the internal connection of two components. For those of ordinary skill in the art, the specific meanings of the above terms in this application can be understood according to the specific circumstances. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of this application should be included in the scope of protection of this application.

Claims

1. A photosynthetic bacteria culture device, characterized in that: It includes an incubator, a accommodating cavity, a plurality of first LED planar light source boards, a plurality of second LED planar light source boards and a submersible flow pump; the accommodating cavity is arranged in the incubator, and the accommodating cavity is vertically inserted in the middle position of the bottom of the incubator, the width of the accommodating cavity is smaller than the width of the incubator, and the incubator and the accommodating cavity are both made of transparent materials; a plurality of the first LED planar light source boards are arranged in the accommodating cavity, and the plurality of the first LED planar light source boards are distributed in two rows, and the light source of each first LED planar light source board faces outward; a plurality of the second LED planar light source boards are evenly distributed on the outer side of the bottom of the incubator, and the light source of each second LED planar light source board faces upward; the submersible flow pump is arranged in the incubator, and is used to push the photosynthetic bacterial liquid to form a circulating reflux.

2. The photosynthetic bacteria culture device according to claim 1, characterized in that: The first LED planar light source board and the second LED planar light source board both include a heat dissipation shell, an LED COB light board and a light homogenizing plate. The heat dissipation shell has an opening on one side. The LED COB light board is arranged in the heat dissipation shell with its light source facing the opening of the heat dissipation shell. The light homogenizing plate seals the opening of the heat dissipation shell.

3. The photosynthetic bacteria culture device according to claim 1, characterized in that: The first LED planar light source panel and the second LED planar light source panel are both LED light sources that emit mixed light of blue light and yellow light.

4. The photosynthetic bacteria culture device according to claim 1, characterized in that: It also includes a heating tube, which is vertically arranged in the incubator.

5. The photosynthetic bacteria culture device according to claim 4, characterized in that: It also includes a pH detector, which is arranged in the incubator.

6. The photosynthetic bacteria culture device according to claim 5, characterized in that: It also includes a PLC control system, which is connected to the first LED flat light source board, the second LED flat light source board, the submersible flow pump, the heating tube and the pH detector.

7. A method for culturing a photosynthetic bacterial liquid, characterized in that: The following steps are involved: A. Weigh 20 parts of sodium acetate, 1 part of sodium glutamate, 10 parts of ammonium chloride, 4 parts of magnesium sulfate, 5 parts of potassium dihydrogen phosphate, 10 parts of sodium chloride, 10 parts of yeast extract powder and 10,000 parts of water according to weight ratio, and place them in an incubator to obtain a culture medium of photosynthetic bacterial liquid; B. Inoculate the seed solution of Rhodopseudomonas palustris and Rhodospirilla crimsonii into the incubator at a ratio of 1:1, with the inoculation amount of 10% to 12% for both. 600 ≥1.860; C. Place the incubator in an open, sunny place outdoors and / or turn on the first LED flat light source panel in the incubator and the second LED flat light source panel outside the incubator. Cultivate according to the preset cultivation cycle. When the OD of the photosynthetic bacterial solution in the incubator reaches 660 When the pH value is greater than 1.5, photosynthetic bacterial solution is obtained.

8. The method for culturing photosynthetic bacteria liquid according to claim 7, characterized in that: The step between step B and step C also includes the following steps: measuring the pH value of the culture medium of the photosynthetic bacteria liquid by a pH detector in the incubator, and adding sodium hydroxide solution to adjust the pH value to 7.

5.

9. The method for culturing photosynthetic bacteria liquid according to claim 7, characterized in that: In step C, the heating tube in the incubator is controlled by a PLC control system, the temperature in the incubator is set to be constant at 28° C. to 36° C., and the start time and stop time of the first LED planar light source panel and the second LED planar light source panel are set.

10. A photosynthetic bacterial liquid, characterized in that: The method according to any one of claims 7 to 9 is used for the preparation.

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