Rotary biological culture and observation device

Abstract

The invention discloses a rotary biological culture and observation device, and belongs to the technical field of biological culture devices, the rotary biological culture and observation device comprises a control display terminal and a control host, and further comprises an environment adjusting system, a culture dish rotating system arranged in the environment adjusting system and an imaging system; the environment adjusting system comprises a culture cabin, the culture cabin comprises a cabin chamber and a cabin door connected with the cabin chamber, and a plurality of culture dish rotating systems are circumferentially distributed in the cabin chamber. By adopting the rotary biological culture and observation device, the culture dish rotating system can optimize a cell growth environment and improve the nutrient exchange rate of biological cells by utilizing centrifugal force generated by rotation and hydrodynamic characteristics, and the environment adjusting system can provide a gas development environment and a temperature development environment for biological sample culture; the phenomena of slow development or death of biological sample cells are reduced, and the culture success rate is increased. Meanwhile, the imaging system can realize real-time imaging and observation of the development state of the biological sample on the premise of not destroying the culture environment.

Description

Technical Field

[0001] The invention relates to the technical field of biological culture devices, in particular to a rotary biological culture and observation device. Background Art

[0002] In the field of biological cell culture, biological sample culture usually adopts static culture method (biological culture samples are statically suspended in nutrients or adsorbed on the inner wall of culture vessels for culture) and uses microscope observation to monitor and evaluate the development status of biological samples. When the biological sample cells develop into cell aggregates, the static culture method will cause the biological sample cells to develop slowly or die because of insufficient nutrient exchange between cells and culture fluid and the culture environment does not meet the requirements of the original organism conditions. At the same time, observing the development status of biological samples under a microscope in a non-culture environment will cause the biological sample cells to develop slowly or die due to sudden changes in the development temperature environment and gas concentration environment. Therefore, in order to avoid the above problems, the prior art adopts a rotating biological culture method combined with a microscope, and uses the centrifugal force and fluid dynamics generated by the rotation to optimize the cell growth environment, which can support the development of biological sample cells into cell aggregates; at the same time, integrated microscope imaging can realize real-time dynamic image monitoring of the development process of biological samples without destroying the development environment. Summary of the invention

[0003] The purpose of the present invention is to provide a rotary biological culture and observation device to solve the above technical problems.

[0004] To achieve the above-mentioned purpose, the present invention provides a rotary biological culture and observation device, comprising a control display terminal and a control host, wherein the control display terminal is electrically connected to the control host, and further comprises an environment adjustment system, a culture dish rotation system arranged in the environment adjustment system, and an imaging system; the environment adjustment system comprises a culture cabin, the culture cabin comprises a cabin and a cabin door connected to the cabin, and a plurality of culture dish rotation systems are circumferentially distributed in the cabin.

[0005] Preferably, a sealing gasket is provided at the contact position between the cabin and the cabin door, and the culture cabin is provided with an air vent.

[0006] Preferably, a temperature regulation subsystem is provided in the culture cabin, and the temperature regulation subsystem includes a cabin door temperature controller and a cabin temperature controller. The cabin door temperature controller is electrically connected to a first heating plate fixed in the cabin door, and the cabin temperature controller is electrically connected to a second heating plate fixed in the cabin. Both the cabin door temperature controller and the cabin temperature controller are electrically connected to a control host.

[0007] Preferably, a gas concentration regulating subsystem is provided in the culture cabin, the gas concentration regulating subsystem includes an air intake controller, a detection component and a uniform fan, the air intake controller, the detection component and the uniform fan are all electrically connected to the control host, the uniform fan and the detection component are both installed in the cabin, the detection component includes an oxygen sensor and a carbon dioxide sensor, and the air intake controller includes a nitrogen vent, an oxygen vent, a carbon dioxide vent and a mixed gas outlet; The oxygen concentration adjustment operation is as follows: Open the nitrogen vent and close the oxygen vent, and reduce the oxygen concentration in the cabin by continuously inputting nitrogen to exhaust the oxygen in the cabin; open the oxygen vent and close the nitrogen vent, and increase the oxygen concentration in the cabin by continuously inputting oxygen; The CO2 regulation operation is as follows: Close the carbon dioxide vent, open the nitrogen vent, and continue to introduce nitrogen into the cabin to reduce the carbon dioxide concentration in the cabin; Open the carbon dioxide vent and increase the carbon dioxide concentration in the cabin by continuously introducing carbon dioxide into the cabin.

[0008] Preferably, the culture dish rotating system comprises a dish holder and a protective casing for connecting the culture dish, the dish holder is mounted on one end of a hollow shaft, the hollow shaft is connected to the protective casing via two bearings, a rotating driven gear is arranged on the hollow shaft between the two bearings, the rotating driven gear is meshed with a rotating driving gear, the rotating driving gear is connected to a transmission shaft, the transmission shaft passes through the protective casing and is connected to a rotating drive motor, and the rotating drive motor is mounted on the protective casing; A photoelectric switch is installed on the rear cover plate of the protective housing, and a detection sheet is installed on the hollow shaft, and the detection sheet and the photoelectric switch are arranged oppositely; The rotary drive motor and the photoelectric switch are both electrically connected to the control host.

[0009] Preferably, the imaging system comprises a light source assembly and a microscope assembly, and the light source assembly is arranged opposite to the microscope assembly.

[0010] Preferably, the light source assembly includes an adjustment plate and a light source, the light source is mounted on the door and arranged opposite to the hollow shaft, a plurality of strip-shaped adjustment through holes distributed circumferentially are arranged on the door, the limiting columns on the adjustment plate are arranged in the strip-shaped adjustment through holes, and the adjustment plate is arranged between the light source and the culture dish; The light source is electrically connected to the control host.

[0011] Preferably, the microscope assembly includes an image collector, a position adjustment mechanism and an imaging adjustment mechanism, the position adjustment mechanism includes a rotating platform installed in the middle of the cabin, a telescopic motor is installed on the rotating platform, the imaging adjustment mechanism is installed on the telescopic end of the telescopic motor, the imaging adjustment mechanism includes a microscope, a focus driven gear and a magnification driven gear arranged on the microscope, the focus driven gear is meshed with a focus driving gear, the focus driving gear is arranged on the focus motor, the magnification driven gear is meshed with a magnification driving gear, the magnification driving gear is arranged on the magnification motor, the magnification motor and the focusing motor are both installed on a mounting frame, the mounting frame is arranged at the telescopic end of the telescopic motor, the image collector is installed on the mounting frame, and the image collector is arranged opposite to the microscope; The image collector, the rotating platform, the telescopic motor, the magnification adjustment motor and the focusing motor are all electrically connected to the control host.

[0012] Therefore, the present invention adopts the above-mentioned rotating biological culture and observation device, which has the following beneficial effects: (1) By setting up a culture dish rotation system, the culture dish can rotate on its own. By setting the corresponding rotation speed, the shear force of the fluid on the cells can be greatly reduced, protecting the cells from damage. During the rotation process, the cultured cells are placed in the center of the culture dish, simulating the microgravity environment in space, which helps the cells grow without restrictions in a three-dimensional environment, similar to the in vivo environment, which is conducive to cell differentiation and tissue formation. During the rotation process, the nutrients and oxygen in the culture medium can be evenly distributed around the cells, ensuring the consistency of nutrient supply within the cell group and promoting uniform cell growth. Through rotation, the gas can be directly dissolved in the culture medium without the need for bubbles, thereby improving the oxygen transfer efficiency and avoiding cell damage caused by bubbles.

[0013] (2) Microscope imaging is achieved while maintaining the culture environment. That is, images of cultured cells are captured during the rotation process to avoid irreversible damage to the cells caused by changes in the culture environment. High-frequency image capture enables real-time developmental dynamic image monitoring without missing key developmental details.

[0014] The technical solution of the present invention is further described in detail below through the accompanying drawings and embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a schematic diagram of the external structure of a rotary biological cultivation and observation device of the present invention; Figure 2 This is a schematic diagram of the internal structure of a rotary biological cultivation and observation device of the present invention; Figure 3 This is a schematic diagram of the structure of the culture dish rotation system of the present invention; Figure 4 It is a cross-sectional view of the culture dish rotating system of the present invention; Figure 5 This is a schematic diagram of the installation position of the photoelectric switch of the present invention; Figure 6 This is a schematic diagram of the concentration adjustment subsystem principle of the present invention; Figure 7 This is a schematic diagram of the temperature regulation subsystem of the present invention; Figure 8 It is a schematic diagram of the structure of the microscope assembly of the present invention.

[0016] Reference numerals 1. Environmental adjustment system; 11. Temperature adjustment subsystem; 111. Door temperature controller; 112. Cabin temperature controller; 113. First heating plate; 114. Second heating plate; 12. Concentration adjustment subsystem; 121. Air intake controller; 121a. Oxygen vent; 121b. Carbon dioxide vent; 121c. Nitrogen vent; 121d. Mixed gas outlet; 122. Detection component; 123. Uniform fan; 13. Culture dish rotation system; 131. Culture dish; 132. Dish support; 133. Hollow shaft; 134. Protective housing; 135. Rotate driven gear; 136. Rotate driving gear; 137. Transmission shaft; 1 38. Rotary drive motor; 139. Detection sheet; 1310. Photoelectric switch; 1311. Bearing; 14. Culture chamber; 141. Chamber; 142. Hatch door; 143. Sealing gasket; 144. Air vent; 2. Imaging system; 21. Light source assembly; 211. Adjustment plate; 212. Light source; 213. Bar adjustment through hole; 214. Limiting column; 22. Microscope assembly; 221. Microscope; 222a. Focus motor; 222b. Magnification motor; 223. Focus driven gear; 224. Magnification driven gear; 225. Image collector; 226. Telescopic motor; 227. Rotating platform; 3. Control host; 4. Control display terminal. DETAILED DESCRIPTION

[0017] Example In the description of the present invention, it should be noted that the terms "upper", "lower", "inside", "outside", etc. indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, or the positions or positional relationships in which the invented product is usually placed when in use. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention. In the description of the present invention, it should also be noted that, unless otherwise clearly specified and limited, the terms "set", "install", and "connect" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be an indirect connection through an intermediate medium, or it can be a connection between the two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0018] The embodiments of the present invention are described in detail below in conjunction with the accompanying drawings.

[0019] like Figure 1-Figure 2 As shown, a rotating biological culture and observation device includes a control display terminal 4, a control host 3, an environment adjustment system 1, a culture dish rotation system 13 arranged in the environment adjustment system 1, and an imaging system 2, and the control display terminal 4 is electrically connected to the control host 3. In this embodiment, the control display terminal 4 is a computer, which is an existing device. The computer can display the image captured by the image acquisition device 225 in the microscope assembly, and the computer can control the environment adjustment system 1, the culture dish rotation system 13 and the imaging system 2 through the control host 3.

[0020] The environment adjustment system 1 includes a culture chamber 14, a temperature adjustment subsystem 11, and a gas concentration adjustment subsystem 12. The culture chamber 14 includes a chamber 141 and a door 142 connected to the chamber 141. A sealing gasket 143 is provided at the contact position between the chamber 141 and the door 142. The sealing gasket 143 ensures that the culture chamber 14 is airtight and leak-proof when the door 142 is closed. The culture chamber 14 is provided with an air vent 144 for discharging excess gas when adjusting the gas concentration in the chamber 141.

[0021] The culture chamber 14 is provided with a temperature regulating subsystem 11 for providing a stable temperature development environment for biological culture. Figure 7 As shown, the temperature regulation subsystem 11 includes a cabin door temperature controller 111 and a cabin temperature controller 112. The cabin door temperature controller 111 is electrically connected to a first heating plate 113 fixed in the cabin door 142. The cabin temperature controller 112 is electrically connected to a second heating plate 114 fixed in the cabin 141. Both the cabin door temperature controller 111 and the cabin temperature controller 112 are electrically connected to the control host 3.

[0022] The culture chamber 14 is provided with a gas concentration regulating subsystem 12 for providing a stable gas concentration development environment for biological culture, such as Figure 6 As shown, the gas concentration regulating subsystem 12 includes an air intake controller 121, a detection component 122 and a uniform fan 123. The air intake controller 121, the detection component 122 and the uniform fan 123 are all electrically connected to the control host 3. The uniform fan 123 and the detection component 122 are installed in the chamber 141 and are respectively used to mix the gas in the culture chamber 14 and detect the corresponding gas concentration. The detection component 122 includes an oxygen sensor and a carbon dioxide sensor. Other gas sensors can also be added according to actual conditions to provide a stable oxygen concentration and carbon dioxide concentration development environment. According to the set concentration parameters, the corresponding gases are mixed to form a mixed gas. The detection component 122 detects the gas concentration of the corresponding gas in the culture chamber 14. The detected gas concentration is compared with the set gas concentration parameter and fed back to the control host 3. The control host 3 performs corresponding regulation on the air intake controller 121 to supplement the corresponding gas into the culture chamber 14, and the excess gas is discharged from the gas outlet 144 of the culture chamber 14 until the gas concentration of the corresponding gas reaches the set concentration. The air intake controller 121 includes a nitrogen vent 121 c , an oxygen vent 121 a , a carbon dioxide vent 121 b , and a mixed gas outlet 121 d , and the mixed gas outlet 121 d is connected to the chamber 141 .

[0023] The oxygen concentration adjustment operation is as follows: The nitrogen vent 121c is opened and the oxygen vent 121a is closed, and the oxygen concentration in the cabin 141 is reduced by continuously inputting nitrogen to discharge the oxygen in the cabin 141; the oxygen vent 121a is opened and the nitrogen vent 121c is closed, and the oxygen concentration in the cabin 141 is increased by continuously inputting oxygen.

[0024] The CO2 regulation operation is as follows: The carbon dioxide vent 121 b is closed, and the nitrogen vent 121 c is opened, and the carbon dioxide concentration in the chamber 141 is reduced by continuously introducing nitrogen into the chamber 141 .

[0025] The carbon dioxide vent 121 b is opened to continuously introduce carbon dioxide into the chamber 141 to increase the carbon dioxide concentration in the chamber 141 .

[0026] Several culture dish rotating systems 13 are circumferentially distributed in the chamber 141 to provide a rotating culture environment for the development of biological samples. The centrifugal force and fluid dynamics generated by the rotation can increase the nutrient exchange rate of biological cells. Figure 3-Figure 4As shown, the culture dish rotating system 13 includes a dish holder 132 and a protective housing 134 for connecting a culture dish 131. The culture and culture liquid are placed in the culture dish 131. A breathable membrane is provided on the culture dish 131 to ensure gas exchange between the inside and outside of the culture dish under the premise of preventing liquid from overflowing. The dish holder 132 is installed at one end of a hollow shaft 133, and light passing through the culture dish 131 can be transmitted through the hollow shaft 133. The hollow shaft 133 is connected to the protective housing 134 through two bearings 1311. A rotating driven gear 135 is provided on the hollow shaft 133 between the two bearings. The rotating driven gear 135 is meshed with a rotating driving gear 136. The rotating driving gear 136 is connected to a transmission shaft 137. The transmission shaft 137 passes through the protective housing 134 and is connected to a rotating driving motor 138. The rotating driving motor 138 is installed on the protective housing 134. A photoelectric switch 1310 is installed on the rear cover plate of the protective housing 134, and a detection sheet 139 is installed on the hollow shaft 133, and the detection sheet 139 is arranged opposite to the photoelectric switch 1310. The rotation drive motor 138 and the photoelectric switch 1310 are both electrically connected to the control host 3. The photoelectric switch 1310 detects the rotation speed of the detection sheet 139 in real time and feeds it back to the control host 3, and the control host 3 corrects the rotation error of the culture dish 131 in real time to achieve precise control of the rotation speed.

[0027] The imaging system 2 includes a light source assembly 21 and a microscope assembly 22. The light source assembly 21 is arranged opposite to the microscope assembly 22. The light source assembly 21 provides light for imaging of biological samples under the microscope 221 and includes an adjustment plate 211 and a light source 212. The light source 212 is mounted on the cabin door 142 and arranged opposite to the hollow shaft. The cabin door 142 is provided with a plurality of strip-shaped adjustment through holes 213 distributed in a circumference. The limiting posts 214 on the adjustment plate 211 are arranged in the strip-shaped adjustment through holes 213. The adjustment plate 211 is arranged between the light source 212 and the culture dish 131. The light source 212 is electrically connected to the control host 3. The adjustment plate 211 can adjust the light source 212 to form a bright field light source 212 and a dark field light source 212, and can adjust the dark field strength and weakness; the control host 3 is electrically connected to the light source 212 and can control the brightness of the light source 212.

[0028] The microscope assembly 22 includes an image collector 225, a position adjustment mechanism and an imaging adjustment mechanism. The position adjustment mechanism is used to adjust the positions of the image collector 225 and the imaging adjustment mechanism, and includes a rotating platform 227 installed in the middle of the cabin 141. A telescopic motor 226 is installed on the rotating platform 227. The telescopic end of the telescopic motor 226 is installed with an imaging adjustment mechanism. The imaging adjustment mechanism includes a microscope 221, a focus driven gear 223 and a magnification driven gear 224 arranged on the microscope 221, and the focus driven gear 223 is meshed with a focus driven gear 224. The driving gear and the focusing driving gear are arranged on the focusing motor 222a, the power adjustment driven gear 224 is meshed with the power adjustment driving gear, the power adjustment driving gear is arranged on the power adjustment motor 222b, the power adjustment motor 222b and the focusing motor 222a are both installed on the mounting frame, the power adjustment motor 222b and the focusing motor 222a respectively adjust the optical imaging magnification and the focal length of the microscope 221 imaging, the mounting frame is arranged at the telescopic end of the telescopic motor 226, and the image collector 225 is installed on the mounting frame, and the image collector 225 is arranged opposite to the microscope 221. The image collector 225, the rotating platform 227, the telescopic motor 226, the power adjustment motor 222b and the focusing motor 222a are all electrically connected to the control host 3. The three-dimensional coordinate positioning of the biological sample in the culture dish 131 is completed by the rotating platform 227, the telescopic motor 226 and the focusing motor 222a. The light source 212 emits light, which passes through the biological sample contained in the culture dish 131, the hollow shaft 133 and the microscope 221 in turn, and is finally collected by the image collector 225, which captures the image of the biological sample contained in the culture dish 131.

[0029] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that they can still modify or replace the technical solution of the present invention with equivalents, and these modifications or equivalent replacements cannot cause the modified technical solution to deviate from the spirit and scope of the technical solution of the present invention.

Claims

1. A rotary biological culture and observation device, comprising a control display terminal and a control host, wherein the control display terminal is electrically connected to the control host, and characterized in that: It also includes an environment adjustment system, a culture dish rotating system arranged in the environment adjustment system, and an imaging system; the environment adjustment system includes a culture cabin, the culture cabin includes a cabin and a cabin door connected to the cabin, and a plurality of culture dish rotating systems are circumferentially distributed in the cabin.

2. A rotary biological culture and observation device according to claim 1, characterized in that: A sealing pad is arranged at the contact position between the cabin and the cabin door, and an air vent is arranged in the culture cabin.

3. A rotary biological culture and observation device according to claim 2, characterized in that: A temperature regulation subsystem is provided in the culture cabin, and the temperature regulation subsystem includes a cabin door temperature controller and a cabin temperature controller. The cabin door temperature controller is electrically connected to a first heating plate fixed in the cabin door, and the cabin temperature controller is electrically connected to a second heating plate fixed in the cabin. Both the cabin door temperature controller and the cabin temperature controller are electrically connected to a control host.

4. A rotary biological culture and observation device according to claim 3, characterized in that: A gas concentration regulating subsystem is provided in the culture chamber, and the gas concentration regulating subsystem includes an air intake controller, a detection component and a uniform fan, the air intake controller, the detection component and the uniform fan are all electrically connected to the control host, the uniform fan and the detection component are both installed in the chamber, the detection component includes an oxygen sensor and a carbon dioxide sensor, and the air intake controller includes a nitrogen vent, an oxygen vent, a carbon dioxide vent and a mixed gas outlet; The oxygen concentration adjustment operation is as follows: Open the nitrogen vent and close the oxygen vent, and reduce the oxygen concentration in the cabin by continuously inputting nitrogen to exhaust the oxygen in the cabin; open the oxygen vent and close the nitrogen vent, and increase the oxygen concentration in the cabin by continuously inputting oxygen; The CO2 regulation operation is as follows: Close the carbon dioxide vent, open the nitrogen vent, and continue to introduce nitrogen into the cabin to reduce the carbon dioxide concentration in the cabin; Open the carbon dioxide vent and increase the carbon dioxide concentration in the cabin by continuously introducing carbon dioxide into the cabin.

5. A rotary biological culture and observation device according to claim 4, characterized in that: The culture dish rotating system includes a dish holder and a protective casing for connecting the culture dish, the dish holder is installed at one end of the hollow shaft, the hollow shaft is connected to the protective casing through two bearings, a rotating driven gear is arranged on the hollow shaft between the two bearings, the rotating driven gear is meshed with a rotating driving gear, the rotating driving gear is connected to a transmission shaft, the transmission shaft passes through the protective casing and is connected to a rotating drive motor, and the rotating drive motor is installed on the protective casing; A photoelectric switch is installed on the rear cover plate of the protective housing, and a detection sheet is installed on the hollow shaft, and the detection sheet and the photoelectric switch are arranged oppositely; The rotary drive motor and the photoelectric switch are both electrically connected to the control host.

6. A rotary biological culture and observation device according to claim 5, characterized in that: The imaging system comprises a light source component and a microscope component, wherein the light source component is arranged opposite to the microscope component.

7. A rotary biological culture and observation device according to claim 6, characterized in that: The light source assembly includes an adjustment plate and a light source. The light source is mounted on the door and arranged opposite to the hollow shaft. The door is provided with a plurality of circumferentially distributed strip-shaped adjustment through holes. The limit posts on the adjustment plate are arranged in the strip-shaped adjustment through holes. The adjustment plate is arranged between the light source and the culture dish. The light source is electrically connected to the control host.

8. The rotating biological culture and observation device according to claim 6, characterized in that: The microscope assembly includes an image collector, a position adjustment mechanism and an imaging adjustment mechanism. The position adjustment mechanism includes a rotating platform installed in the middle of the cabin, a telescopic motor is installed on the rotating platform, and the imaging adjustment mechanism is installed on the telescopic end of the telescopic motor. The imaging adjustment mechanism includes a microscope, a focus driven gear and a magnification driven gear arranged on the microscope, the focus driven gear is meshed with a focus driving gear, the focus driving gear is arranged on the focus motor, the magnification driven gear is meshed with a magnification driving gear, the magnification driving gear is arranged on the magnification motor, the magnification motor and the focusing motor are both installed on a mounting frame, the mounting frame is arranged at the telescopic end of the telescopic motor, the mounting frame is installed with an image collector, and the image collector is arranged relative to the microscope; The image collector, the rotating platform, the telescopic motor, the magnification adjustment motor and the focusing motor are all electrically connected to the control host.

Images