Pathological tissue sample slicing device

Abstract

The pathological tissue sample slicing device comprises a storage chamber and a slice taking mechanism, a piston is slidably connected into the storage chamber, the piston is connected with a push rod penetrating through one end of the storage chamber, and the end, away from the push rod, of the storage chamber is provided with an opening; a cutter for slicing the pathological tissue sample is arranged above the opening, and one end, close to the opening, of the storage chamber is communicated with a liquid nitrogen storage tank through a liquid nitrogen guide pipe; the glass slide taking mechanism comprises a rotating shaft, and a plurality of retainers used for fixing glass slides are connected in the circumferential direction of the rotating shaft. According to the scheme, the frozen and solidified pathological tissue sample does not need to be taken out of the storage cavity and then sliced, and the problem of pollution caused in the sample taking process is avoided. When the scheme is used for curing and slicing the pathological tissue sample, compared with the prior art, the use convenience and the working efficiency are improved.

Description

Technical Field

[0001] The invention belongs to the field of frozen sectioning in pathology, and in particular relates to a pathological tissue sample sectioning device. Background Art

[0002] Frozen sections are a method of rapidly cooling tissue to a certain hardness under low temperature conditions and then slicing it. Because its production process is faster and simpler than paraffin sections, it is often used for rapid pathological diagnosis during surgery. There are many types of frozen sections, including low-temperature constant temperature freezer frozen section method, carbon dioxide frozen section method, etc.

[0003] Frozen sections can quickly help doctors diagnose patients' pathology, but the pathological tissue needs to be frozen first when performing frozen sections. The existing freezing method is to freeze the pathological tissue in a freezing incubator. This freezing method involves placement and removal operations, which can easily cause the frozen pathological tissue to be contaminated. Part of the tissue surface needs to be removed before it can be used. When cutting pathological tissue, the cutter needs to be constantly adjusted to be close to the pathological tissue, which takes a lot of time to perform the cutting operation, which in turn causes the preparation of pathological sections to take a long time. Summary of the invention

[0004] The present invention is intended to provide a pathological tissue sample slicing device to solve the problem in the prior art that frozen pathological tissue samples may be contaminated during the slicing process.

[0005] A pathological tissue sample slicing device in the present solution includes a storage chamber and a slice taking mechanism. A piston is slidably connected in the storage chamber, and the piston is connected to a push rod passing through one end of the storage chamber. The end of the storage chamber away from the push rod is an opening, and a cutter for slicing the pathological tissue sample is provided above the opening. The end of the storage chamber close to the opening is connected to a liquid nitrogen storage tank through a liquid nitrogen conduit; the slice taking mechanism includes a rotating shaft, and a plurality of retainers for fixing glass slides are connected to the rotating shaft on the circumference.

[0006] The working principle of the present invention is: the pathological tissue sample is placed in a storage chamber, and liquid nitrogen is introduced into the chamber to freeze the pathological tissue and quickly solidify it. The solidified pathological tissue sample does not need to be taken out of the storage chamber, and the piston is pushed by the push rod, and the piston pushes the solidified pathological tissue sample close to the slide taking mechanism, and the rotating shaft is rotated to make the solidified pathological tissue sample abut against the slide, and then the cutter is used to cut it, and the cut pathological tissue sample slice is attached to the slide.

[0007] The beneficial technical effects of the present invention are: 1. In this solution, the frozen and solidified pathological tissue samples do not need to be taken out of the storage chamber and then sliced, thereby avoiding the problem of contamination caused during the sample taking process.

[0008] 2. The contamination of the pathological tissue sample is avoided, and the step of cutting out the contaminated layer on the surface of the pathological tissue sample is omitted.

[0009] 3. The pathological tissue samples after sectioning are directly attached to the slide. Compared with the existing technology, the work of transferring the sections to the slide is reduced, and the convenience of experimental operation is improved.

[0010] 4. The position of the cutter is fixed. It only needs to push the solidified pathological tissue sample closer to the slice taking mechanism. There is no need to constantly adjust the cutter when cutting the pathological tissue.

[0011] 5. Compared with the existing technology, the present invention is more convenient to use and more efficient in solidifying and slicing pathological tissue samples.

[0012] Furthermore, the holder includes a holding plate, on which a clamp is provided for clamping the glass slide on the holding plate. The glass slide is clamped and fixed by the clamping force between the clamp and the holding plate, which has strong stability and is not easy to fall off, and the operation of clamping and removing the glass slide is also simple.

[0013] Furthermore, the slice taking mechanism further comprises a horizontally arranged slide rail, a bracket is slidably connected to the slide rail, and the rotating shaft is rotatably connected to the bracket. The thickness of the slice can be fine-tuned by sliding the bracket to meet the actual needs of different experimental situations.

[0014] Furthermore, scale lines are provided on the length direction of the slide rail, through which the thickness of the slice can be clearly and quickly determined.

[0015] Furthermore, a locking member is provided between the bracket and the slide rail to fix the bracket and the slide rail during the slicing process to prevent the bracket from sliding easily due to external force during the slicing process.

[0016] Furthermore, the locking member is a bolt, a threaded hole is provided on the bracket, the threaded hole is located beside the slide rail, and the bolt is threadedly connected in the threaded hole.

[0017] Furthermore, a spring in a compressed state is provided between the end of the storage chamber away from the opening and the piston, and the spring is sleeved on the push rod. The end of the push rod away from the piston passes through the storage chamber and is connected to a pull plate perpendicular to the push rod, and the edge of the pull plate exceeds the edge of the push rod. The spring in a compressed state has an active restoring force, and under the action of the restoring force, the piston is pushed to make the pathological tissue slide in the direction of the film taking mechanism, further improving the convenience of the solution.

[0018] Furthermore, a slot is provided on the upper surface of the middle part of the push rod, and a thrust block is provided in the slot. When the pathological tissue sample is frozen, the thrust block is inserted into the slot and abuts against the outer wall of the storage chamber. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is a schematic diagram of the external structure of a pathological tissue sample slicing device of the present invention;

[0020] Figure 2 The figure is a schematic diagram of the internal structure of a pathological tissue sample slicing device of the present invention. DETAILED DESCRIPTION

[0021] The following is further described in detail through specific implementation methods:

[0022] The figure marks in the drawings of the specification include: pull plate 1, push rod 2, storage chamber 3, spring 4, piston 5, liquid nitrogen tube 6, cutter 7, clip 8, slide 9, retaining plate 10, rotating shaft 11, bracket 12, slide rail 13, bolt 14, table 15, operating table 16.

[0023] The embodiment is basically as shown in the attached Figure 1 and Figure 2 As shown: a pathological tissue sample slicing device, including an operating table 16, on which a storage chamber 3 and a slicing mechanism are provided, a piston 5 is slidably connected in the storage chamber 3, the piston 5 is connected to a push rod 2 passing through one end of the storage chamber 3, the end of the storage chamber 3 away from the push rod 2 is an opening, a baffle door is provided near the opening of the storage chamber 3 to close the opening after sliding downward from the top, a cutter 7 is rotatably provided on one side of the opening, and the frozen pathological tissue sample is sliced ​​when the cutter 7 is cut, a pathological tissue sample placement port is provided at the top of the storage chamber 3 near the opening, and a transparent cover body is provided at the placement port, through which the pathological tissue inside the storage chamber 3 can be observed. The end of the storage chamber 3 near the opening is connected to a liquid nitrogen storage tank (not shown in the figure) through a liquid nitrogen conduit 6.

[0024] A spring 4 in a compressed state is provided between the end of the storage chamber 3 away from the opening and the piston 5. The spring 4 is sleeved on the push rod 2. The end of the push rod 2 away from the piston 5 passes through the storage chamber 3 and is connected to a pull plate 1 perpendicular to the push rod 2. The edge of the pull plate 1 exceeds the edge of the push rod 2. A slot is provided on the upper surface of the middle part of the push rod 2. A thrust block is provided in the slot. When the pathological tissue sample is frozen, the thrust block is inserted into the slot and abuts against the outer wall of the storage chamber 3.

[0025] The film taking mechanism includes a rotating shaft 11, and a plurality of holders for fixing the glass slide 9 are connected to the rotating shaft 11 in the circumferential direction. The holder includes a holding plate 10, and the holding plate 10 is provided with a clamping piece 8 for clamping the glass slide 9 on the holding plate 10. The film taking mechanism also includes a horizontally arranged table 15 and a slide rail 13 on the table 15, and an escape opening for the cutter 7 to cut is formed between the table 15 and the bottom of the storage chamber 3. A bracket 12 is slidably connected to the slide rail 13, and the rotating shaft 11 is rotatably connected to the bracket 12. A locking member is provided between the bracket 12 and the slide rail 13, and the locking member is a bolt 14. A threaded hole is provided on the bracket 12, and the threaded hole is located beside the slide rail 13. The bolt 14 is threadedly connected in the threaded hole. Preferably, a scale line can be set in the length direction of the slide rail 13, and the thickness of the slice can be clearly and quickly determined through the scale line.

[0026] The specific implementation process is as follows: Figure 1 In the state shown, the cover is opened, the pathological tissue sample is placed in the storage chamber 3, the cover is closed, the baffle door closes the opening, and then liquid nitrogen is introduced into the storage chamber 3 through the liquid nitrogen conduit 6 to freeze the pathological tissue and quickly solidify. The solidified pathological tissue sample does not need to be taken out from the storage chamber 3. The piston 5 is pushed by the push rod 2, and the piston 5 pushes the solidified pathological tissue sample close to the film taking mechanism, and the baffle door is removed. The rotating shaft 11 is rotated to make one of the slides 9 abut against the bottom of the rotating shaft 11, and the thrust block is removed. The push rod 2 pushes the piston 5 and the solidified tissue to move toward the slide 9 under the restoring force of the spring 4, and the solidified pathological tissue sample abuts against the slide 9, and then the cutter 7 is cut, and the cut pathological tissue sample slice is attached to the slide 9. While pulling the pull plate 1 to prevent the push rod 2 from moving, the empty slide 9 is rotated again under the rotating shaft 11, and the above operation is repeated to make multiple slices.

Claims

1. A pathological tissue sample slicing device, characterized in that: The utility model comprises a storage chamber and a film taking mechanism. A piston is slidably connected in the storage chamber, and the piston is connected to a push rod passing through one end of the storage chamber. The end of the storage chamber away from the push rod is an opening, and a cutter for slicing pathological tissue samples is arranged above the opening. The end of the storage chamber close to the opening is connected to a liquid nitrogen storage tank through a liquid nitrogen conduit. The film taking mechanism comprises a rotating shaft and a plurality of retainers for fixing the slides connected to the circumference of the rotating shaft.

2. A pathological tissue sample slicing device according to claim 1, characterized in that: The holder comprises a holding plate, on which a clamping piece for clamping the glass slide on the holding plate is provided.

3. A pathological tissue sample slicing device according to claim 1, characterized in that: The film taking mechanism also includes a horizontally arranged slide rail, a bracket is slidably connected to the slide rail, and the rotating shaft is rotatably connected to the bracket.

4. A pathological tissue sample slicing device according to claim 3, characterized in that: Scale lines are arranged in the length direction of the slide rail.

5. The pathological tissue sample slicing device according to claim 3, characterized in that: A locking piece is arranged between the bracket and the slide rail.

6. A pathological tissue sample slicing device according to claim 5, characterized in that: The locking member is a bolt, a threaded hole is provided on the bracket, the threaded hole is located beside the slide rail, and the bolt is threadedly connected in the threaded hole.

7. A pathological tissue sample slicing device according to any one of claims 1 to 6, characterized in that: A compressed spring is provided between the end of the storage chamber away from the opening and the piston, and the spring is sleeved on the push rod. The end of the push rod away from the piston passes through the storage chamber and is connected to a pull plate perpendicular to the push rod, and the edge of the pull plate exceeds the edge of the push rod.

8. The pathological tissue sample slicing device according to claim 7, characterized in that: A slot is provided on the upper surface of the middle part of the push rod, and a thrust block is provided in the slot. When the pathological tissue sample is frozen, the thrust block is inserted into the slot and abuts against the outer wall of the storage chamber.

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