DNA computing system structure based on DNA nanostructure dynamic assembly and preparation method

Abstract

The invention discloses a DNA computing system structure based on DNA nanostructure dynamic assembly and a preparation method, and belongs to the field of DNA nanostructures. The structure comprises a DNA central processor, a DNA memory, an input device and an output device. The DNA central processing unit comprises a DNA controller and a DNA arithmetic unit; the DNA controller is of a central DNA tetrahedral nanostructure, and the DNA arithmetic unit is of an edge DNA tetrahedral nanostructure; the central DNA tetrahedron nanostructure and the plurality of edge DNA tetrahedron nanostructures form a DNA tetrahedron polymer nanostructure through complementary pairing of extension chain bases; the DNA memory is addressable DNA origami, is of an asymmetric rectangular two-dimensional DNA nanostructure, and comprises a DNA template chain and a DNA staple chain; a capture chain is anchored on the surface of the structure to be complementarily paired with an extension chain base; and the structure is provided with an asymmetric DNA origami replacement chain.

Description

Technical Field

[0001] The present invention belongs to the field of DNA nanostructures, and in particular relates to a DNA computing system structure and a preparation method based on the dynamic assembly of DNA nanostructures. Background Art

[0002] Quantum computers have powerful information processing capabilities, but lack accuracy and error correction capabilities. Because DNA molecules have precise base complementary pairing characteristics, DNA-based biological computers greatly reduce data error rates. In addition, DNA computing can complete 1020 operations in parallel, and its computing speed and parallel capabilities far exceed those of silicon-based computers; the physical density of the DNA storage system is 215 PB / g, which is six orders of magnitude higher than current storage media. Thanks to the unique advantages of DNA computing, as well as the inherent advantages of DNA such as low cost, easy synthesis, good biocompatibility and high programmability, DNA computing has received widespread attention and rapid development in recent decades, becoming one of the most outstanding candidates for molecular computers.

[0003] At present, the development of DNA computers is mainly focused on the design and optimization of DNA circuits. DNA circuits are molecular information regulation and processing technologies, and are the basic components of DNA computers to realize logical operations and logical functions. Logical operations are the basis of molecular computing, and are also the basic components of various complex logic devices, playing a vital role in various multifunctional logic devices. Although DNA circuits based on ssDNA have successfully realized complex logical operations at the molecular level. However, existing research is still limited to the development of "operator" components in computer systems, and has not formed a complete computer structure. In addition, logic circuits designed based on ssDNA usually use electrophoresis technology and fluorescence analysis technology to monitor changes in electrophoresis bands and fluorescence intensity to analyze the results of logical operations. The types of operations for multi-threaded processing are limited, which limits the computing power of DNA computers. With the help of single-molecule imaging technology, the output operation results / information are presented at the single-molecule level, which is expected to realize multi-threaded operations and outputs of DNA computing systems and improve computing efficiency. Summary of the invention

[0004] In view of the deficiencies in the prior art, the purpose of the present invention is to provide a DNA computing system structure and a preparation method based on the dynamic assembly of DNA nanostructures, which solves the problems in the prior art.

[0005] The purpose of the present invention can be achieved through the following technical solutions: The DNA computing architecture based on the dynamic assembly of DNA nanostructures includes a DNA central processor, a DNA memory, an input device and an output device; the DNA central processor includes a DNA controller and a DNA operator; The DNA controller is a central DNA tetrahedron nanostructure, and the DNA operator is an edge DNA tetrahedron nanostructure; a central DNA tetrahedron nanostructure and a plurality of edge DNA tetrahedron nanostructures form a DNA tetrahedron polymer nanostructure through complementary pairing of extended chain bases, i.e., a DNA central processor; The DNA memory is an addressable DNA origami in the form of an asymmetric rectangular two-dimensional DNA nanostructure, comprising a DNA template chain and a DNA staple chain; a plurality of capture chains are anchored on the surface of the structure, and are respectively complementary to the extended chain bases of different edge DNA tetrahedral nanostructures; an asymmetric DNA origami replacement chain is arranged on the asymmetric rectangular two-dimensional DNA nanostructure.

[0006] Furthermore, the central DNA tetrahedral nanostructure and the edge DNA tetrahedral nanostructure are both composed of four DNA backbone chains, each DNA backbone chain is stacked to form a triangle, constituting each face of the DNA tetrahedral nanostructure, and each edge of the central DNA tetrahedral nanostructure and the edge DNA tetrahedral nanostructure is composed of two DNA chains with complementary base pairing.

[0007] Furthermore, in the DNA tetrahedral polymer nanostructure, the number of edge DNA tetrahedral nanostructures is four; the number of capture chains on the surface of the asymmetric rectangular two-dimensional DNA nanostructure is four, and they are respectively complementary to the extended chains of the four edge DNA tetrahedral nanostructures; The four vertices of the central DNA tetrahedron nanostructure are respectively connected to an extended chain, and one of the vertices of the four edge DNA tetrahedron nanostructures is respectively connected to an extended chain, with a total of 8 extended chains; the four extended chains on the central DNA tetrahedron nanostructure are respectively complementary to the extended chains on the four edge DNA tetrahedron nanostructures in base pairing.

[0008] Furthermore, the sequences of the four DNA backbone chains are respectively shown as: SEQ ID NO.1-SEQ ID NO.4; the sequences of the four extended chains on the four edge DNA tetrahedral nanostructures are respectively shown as SEQ ID NO.5-SEQ ID NO.8; the sequences of the four extended chains on the central DNA tetrahedral nanostructure are respectively shown as SEQ ID NO.9-SEQ ID NO.12.

[0009] Furthermore, the DNA template strand is a M13mp18 phage genomic DNA sequence; the M13mp18 phage genomic DNA sequence is shown in SEQ ID NO.13; Furthermore, the nucleotide sequences of the four capture chains are shown in SEQ ID NO.32-SEQ ID NO.35 respectively.

[0010] Furthermore, the nucleotide sequence of the asymmetric DNA origami replacement chain is shown in SEQ ID NO.14-SEQ ID NO.31.

[0011] The method for preparing the DNA computing architecture based on the dynamic assembly of DNA nanostructures comprises the following steps: S1, the DNA template strand, DNA staple strand, asymmetric DNA origami replacement strand and capture strand are mixed in a buffer solution, and an asymmetric rectangular two-dimensional DNA nanostructure is obtained after annealing and ultrafiltration purification; S2, mixing the DNA backbone chains in a buffer, annealing, and ultrafiltration purification to obtain central DNA tetrahedral nanostructures and edge DNA tetrahedral nanostructures with different extended chains; S3, mixing the central DNA tetrahedral nanostructure and the edge DNA tetrahedral nanostructure in a buffer solution, and annealing to obtain a DNA tetrahedral polymer nanostructure.

[0012] Furthermore, in S1, the buffer was 1×TAE / Mg 2+ buffer, in the mixed solution, the concentration of the DNA template strand was 10 nM, and the total concentration of the DNA staple strand, the asymmetric DNA origami replacement strand, and the DNA capture strand was 80 nM; The buffer in S2 and S3 is TM buffer; and in S3, the five DNA tetrahedral nanostructures have equal molar ratios.

[0013] The above-mentioned DNA computing architecture based on dynamic assembly of DNA nanostructures is applied in miRNA detection.

[0014] Beneficial effects of the present invention: (1) The present invention uses a simpler and more effective system based on the strand displacement method to achieve dynamic conversion of DNA nanostructures; (2) The present invention provides a construction method and application of a DNA computing system based on a dynamic DNA nanostructure, using the DNA nanostructure as a computing module of the DNA computing system architecture. The designed DNA nanostructure has uniform size, recombinant programmable sites provide precise node site design, and abundant extensible side chains can provide stable and reliable physical connection relationships. (3) The present invention provides a method for constructing and applying a DNA computing system based on a dynamic DNA nanostructure, which has the function of detecting multiple miRNAs. The DNA fuel chain used is a DNA chain equivalent to miRNA. The present invention can also detect multiple miRNAs efficiently and conveniently.

[0015] (4) The asymmetric rectangular two-dimensional DNA nanostructure and DNA tetrahedral nanostructure mainly used in the present invention have good biocompatibility, and other required chemical and biological materials are also non-toxic to the human body. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, for ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0017] Figure 1 The flowchart of the operation of the DNA computing system based on the dynamic assembly of DNA nanostructures of the present invention; Figure 2 A schematic diagram of node information encoding in the present invention; Figure 3 This is a PAGE experimental result diagram of the DNA computing device and DNA controller in the present invention; Figure 4 This is a diagram of the PAGE experimental results of the DNA central processing unit in the present invention; Figure 5 This is a morphological representation diagram of the DNA central processing unit in the present invention; Figure 6 for Figure 5 The enlarged schematic diagram at A in the middle; Figure 7 This is a morphological characterization diagram of the DNA memory in the present invention; Figure 8 It is a PAGE experimental result diagram of the operation process in the present invention; Fig. 9 It is a morphological representation diagram of the calculation result in the present invention. DETAILED DESCRIPTION

[0018] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0019] Example 1 The DNA computing architecture based on the dynamic assembly of DNA nanostructures includes a DNA central processing unit module, a DNA memory module, an input device and an output device; the DNA central processing unit module includes a DNA controller and a DNA operator; The DNA central processor module is: a DNA tetrahedral polymer nanostructure that dynamically responds to input information; the DNA controller is the central DNA tetrahedral nanostructure in the DNA tetrahedral polymer nanostructure; the DNA operator is the four edge DNA tetrahedral nanostructures connected to the central DNA tetrahedral nanostructure; the DNA tetrahedral polymer nanostructure (DNA central processor module) is: composed of a central DNA tetrahedral nanostructure (DNA controller) and four edge DNA tetrahedral nanostructures (DNA operator) through extended chain base complementary pairing.

[0020] In this embodiment, the central DNA tetrahedron nanostructure and the edge DNA tetrahedron nanostructure are both composed of four DNA backbone chains, each of which is stacked to form a triangle to form each face of the DNA tetrahedron nanostructure, and each edge of the DNA tetrahedron nanostructure is composed of two DNA chains with complementary base pairing. In addition, the four vertices of the central DNA tetrahedron nanostructure are respectively connected to an extended chain, and one of the vertices of the four edge DNA tetrahedron nanostructures is respectively connected to an extended chain, with a total of 8 extended chains; the four extended chains on the central DNA tetrahedron nanostructure are respectively complementary base pairing with the extended chains on the four edge DNA tetrahedron nanostructures.

[0021] The sequences of the four DNA backbone chains that make up the DNA tetrahedral nanostructure are shown as SEQ ID NO.1-SEQ ID NO.4; the sequences of the four extended chains on the four edge DNA tetrahedral nanostructures (DNA operators) are shown as SEQ ID NO.5-SEQ ID NO.8; the sequences of the four extended chains on the central DNA tetrahedral nanostructure (DNA controller) are shown as SEQ ID NO.9-SEQ ID NO.12.

[0022] The DNA memory module is an addressable DNA origami, which is an asymmetric rectangular two-dimensional DNA nanostructure, which includes a DNA template chain and a DNA staple chain; the DNA template chain is the M13mp18 phage genome DNA sequence; the M13mp18 phage genome DNA sequence is shown in SEQ ID NO.13; The asymmetric rectangular two-dimensional DNA nanostructure partially replaces and adds a portion of the DNA staple chain to form an asymmetric DNA origami replacement chain, so that the rectangular two-dimensional DNA nanostructure has asymmetry. The nucleotide sequences replaced and added by the asymmetric rectangular two-dimensional DNA nanostructure (i.e., the asymmetric DNA origami replacement chain) are shown in SEQ ID NO.14-SEQ ID NO.31. The asymmetric rectangular two-dimensional DNA nanosurface is anchored with four capture chains, each capture chain sequence is base-complementarily paired with the extended chain sequence of the DNA tetrahedral nanostructure (edge ​​DNA tetrahedral nanostructure), and each capture chain captures a DNA tetrahedral nanostructure; the nucleotide sequences of the four capture chains are shown in SEQ ID NO.32-SEQ ID NO.35 respectively.

[0023] The input device is implemented by the instruction DNA chain, which is a single-stranded structure and can also be called a DNA fuel chain. Based on chain displacement, in the catalytic DNA computing architecture, the input DNA fuel chain can be recycled multiple times, and the input DNA fuel chain can directly react with the reaction system to produce output products. The function of maintaining its own operation is maintained without the reaction system being consumed.

[0024] In this embodiment, the nucleotide sequence of the instruction DNA chain is shown as SEQ ID NO.36-SEQ ID NO.39.

[0025] Example 2 In this embodiment, a method for preparing a DNA computing architecture based on dynamic assembly of DNA nanostructures is proposed, and the raw materials and reagents involved in the preparation process are described as follows: 1. Tris (hydroxymethyl) aminomethane (Tris), disodium ethylenediaminetetraacetate (EDTA), magnesium acetate, glacial acetic acid, and boric acid were purchased from Sinopharm Chemical Reagent Co., Ltd.; ultrapure water was used in the experiments.

[0026] 2. 30 kDa and 100 kDa ultrafiltration tubes were purchased from Pall China Company.

[0027] 3. M13mp18 phage genomic DNA was purchased from Biobiotech Co., Ltd. with the product number and specification of B3003-50 pmol; the DNA template chain and DNA staple chain were purchased from Shanghai Sangon Biotechnology Co., Ltd.; the DNA backbone chain, instruction DNA chain, and capture chain were purchased from GenScript Biotech Co., Ltd.

[0028] 4. The mother solution used in the polyacrylamide gel electrophoresis (PAGE) experiment was 30% acrylamide / N,N'-methylbisacrylamide (Acr / Bis) solution purchased from Shanghai Sangon Biotechnology Co., Ltd.

[0029] The experimental reaction of Example 2 was carried out in 1×TAE-Mg 2+ Buffer (40 mM Tris, 20 mM glacial acetic acid, 2 mM EDTA and 12.5 mM magnesium acetate tetrahydrate, pH = 8.0) and TM buffer (10 mM Tris, 5 mM magnesium chloride hexahydrate, pH = 8.0).

[0030] Polyacrylamide gel electrophoresis (PAGE) experimental detection method: Prepare 8% PAGE gel (3.8 L ultrapure water, 1 mL mother solution, 80 μL 10% ammonium persulfate (APS) solution, 8 μL tetramethylethylenediamine (TEMED) solution). Prepare 5% PAGE gel (4.4 mL distilled water, 1 mL 30% PAGE solution, 100 μL 10% ammonium persulfate (APS) solution, 6 μL tetramethylethylenediamine (TEMED) solution).

[0031] If no specific techniques or conditions are specified in the examples, the techniques or conditions described in the literature in the field or the product instructions are used. If no manufacturer is specified for the reagents or instruments used, they are all conventional products that can be obtained through regular channels.

[0032] The method for preparing a DNA computing architecture based on dynamic assembly of DNA nanostructures comprises the following steps: S1, preparation of DNA CPU; Four DNA backbone chains for constructing tetrahedral nanostructures are mixed, the sequences of the DNA backbone chains of the four DNA tetrahedral nanostructures are respectively shown as SEQ ID NO.1-SEQ ID NO.4, and the extended chain sequences of the DNA tetrahedral nanostructures are respectively shown as SEQ ID NO.5-SEQ ID NO.12; wherein the DNA controller and the DNA operator use DNA chains with different extended chains to synthesize the DNA tetrahedral nanostructures, and the final concentration of the DNA tetrahedral nanostructures is set to 1000 nM; 2) The mixed DNA backbone chains were annealed using a PCR instrument under the following annealing conditions: 95°C for 10 min, then lowered to 4°C for 20 min, to obtain different DNA tetrahedral nanostructures; 3) After the annealing process, the DNA tetrahedral nanostructures were taken out and separated using a 30 kDa ultrafiltration tube to remove the unsynthesized DNA backbone chains; the centrifugation conditions were: 300 μL TM buffer was added to 100 μL sample, centrifuged at 3000 rcf / min for 10 min, and repeated for 3 times; finally, the collected DNA tetrahedral nanostructures were placed in different centrifuge tubes. The DNA tetrahedral nanostructures were then quantified using UV spectroscopy.

[0033] The tetrahedral synthesis effect was verified by PAGE experiment, such as Figure 3Lane 1: DNA molecular weight standard Marker (25-500 bp); Lane 2: One nucleotide chain that makes up a DNA tetrahedron nanostructure; Lane 3: Two nucleotide chains that make up a DNA tetrahedron are paired with each other; Lane 4: Three nucleotide chains that make up a DNA tetrahedron are paired with each other; Lane 5: Four nucleotide chains that make up a DNA tetrahedron are paired with each other, i.e., a DNA tetrahedron nanostructure without an extended chain; Lanes 6-9: DNA tetrahedrons with one different extended chain, i.e., a DNA operator; Lane 10: DNA tetrahedron nanostructures with four different extended chains, i.e., a DNA controller; 4) Five DNA tetrahedral nanostructures (one central DNA tetrahedral nanostructure and four edge DNA tetrahedral nanostructures) were added into a centrifuge tube at a molar ratio of 1:1:1:1:1 per unit site. The annealing conditions were: 37°C for 2 h, and the structure concentration was set to 250 nM.

[0034] The PAGE experiment was used to verify the synthesis effect of DNA tetrahedral polymer nanostructure, i.e. DNA CPU. Figure 4 Lane 1: DNA molecular weight standard Marker (100-1200 bp); Lanes 2-5: Four DNA tetrahedral nanostructures with one different extension chain, i.e., DNA computing device; Lane 6: DNA tetrahedral nanostructure with four different extension chains, i.e., DNA controller; Lanes 7-10: DNA tetrahedral dimer nanostructures in which DNA controllers and DNA computing devices are connected in pairs; Lane 11: DNA tetrahedral polymer nanostructure, i.e., DNA central processing unit structure.

[0035] Atomic force microscopy experiments were performed to verify the synthesis effect of DNA tetrahedral polymer nanostructures, i.e., DNA CPUs. Figure 5 and Figure 6 As shown, it can be seen that this structure has five DNA tetrahedral nanostructures, and from the image it can also be seen that its synthesis effect is consistent with the expected design structure.

[0036] S2, constructing DNA memory modules; The preparation raw materials include: M13mp18 template chain and DNA short chain shown in SEQ ID NO.13, the DNA short chain includes: DNA staple chains with 180 sequences, 18 asymmetric DNA origami replacement chains shown in SEQ ID NO.14-SEQ ID NO.31 (making the rectangular two-dimensional DNA nanostructure asymmetric), and 4 DNA capture chains with sequences shown in SEQ ID NO.32-SEQ ID NO.35; The 180 sequences of DNA staple strands are selected from the "Supporting information" of "Shen, C; Lan, X; Lu, X; Ni, W; Wang, Q. Tuning the structural asymmetries of three-dimensional gold nanorod assemblies. Chemical Communications 2015, 51 (71), 13627-13629." Specifically, the 180 sequences are selected from the sequences 1-99 and 112-204 in the "Sequence of staple strands used in the assembly of basic rectangular DNA origami (left to right 5'-3')" disclosed in the above document after removing the sequences 25, 60, 70, 113, 133, 135, 157, 159, 162, 174, 181, and 183; 1) Mix the above DNA strands in 1×TAE / Mg 2+ The total volume after mixing was 100 μL in the buffer, in which the concentration of the M13mp18 template chain was 10 nM, the DNA short chains of each sequence were added in equal amounts and in excess, and the total concentration of the DNA short chains was 80 nM; 2) Use a gradient PCR instrument to slowly anneal the mixture of the M13mp18 template chain and the DNA short chain. The annealing conditions are as follows: starting temperature 95 °C, maintained for 3 min, end temperature 25 °C, each 1 °C as a gradient, each gradient stay 100 s, annealing from 95 °C to 25 °C within two hours, 3) Take out the sample and centrifuge it in a 100 kDa centrifuge tube to remove excess DNA short chains; the centrifugation conditions are: add 300 μL 1×TAE-Mg 2+ Buffer, centrifuge at 3000 rcf / min for 10 min, repeat centrifugation 3 times; The final collected samples were observed using atomic force microscopy (AFM) to observe the morphology of the layer structure. Figure 7 As shown, the constructed DNA nanostructure is a rectangular sheet structure. AFM characterization results show that the rectangular two-dimensional DNA nanostructure is about 90 nm long, about 60 nm wide, and asymmetric.

[0037] Example 3 In this embodiment, the use of a DNA computing system for dynamic computation in a simulated von Neumann system is disclosed; The operation process of the DNA operator is operated through DNA chain displacement; the four edge DNA tetrahedral nanostructures are connected to the central DNA tetrahedral nanostructure through base complementary pairing, and the four chains extending from the central DNA tetrahedral nanostructure have four different DNA chain displacement footholds.

[0038] like Figure 2 As shown, preset conditions are obtained according to the items to be calculated, and the capture sites of the asymmetric rectangular two-dimensional DNA nanostructures are encoded according to the preset conditions; the preset conditions include the number of edge DNA tetrahedral nanostructures, i.e., DNA computing units, the connection between the central DNA tetrahedral nanostructure and the edge DNA tetrahedral nanostructure, and the sequence and number of instruction DNA chains, i.e., DNA fuel chains.

[0039] like Figure 1 As shown, the DNA nanostructure module (DNA tetrahedral polymer nanostructure, i.e., DNA central processor) constructed according to preset conditions, the instruction DNA chain, and the DNA memory are mixed in the DNA computing architecture, annealed, diluted to a certain concentration, and then detected by a detector to obtain the calculation result.

[0040] The annealing conditions are: starting temperature 45°C, end temperature 25°C, each 1°C is a gradient, each gradient stays for 5 min, and 5 cycles are performed. The molar ratio of the instruction DNA chain to the DNA nanostructure module (DNA tetrahedral polymer nanostructure, i.e., DNA central processor) is 10:1; the instruction DNA chain is shown in SEQ ID NO.36-SEQ ID NO.39.

[0041] The PAGE experiment was used to verify the effect of the DNA computing process. Figure 8 Lane 1: DNA molecular weight standard Marker (100-1200 bp); Lane 2: DNA tetrahedron five-mer high-order DNA nanostructure, i.e., DNA central processor; Lanes 3-6: calculation results of adding one displacement chain according to preset conditions; Lanes 7-12: calculation results of adding two displacement chains according to preset conditions; Lane 13: DNA molecular weight standard Marker (100-1200 bp); Lane 14: DNA tetrahedron five-mer high-order DNA nanostructure, i.e., DNA central processor as control; Lanes 15-19: calculation results of adding three displacement chains according to preset conditions; Lane 19: calculation results of adding four displacement chains according to preset conditions; Lane 20: DNA tetrahedron nanostructure with four different extension chains, i.e., DNA controller, as control group; Lanes 21-24: four DNA tetrahedron nanostructures with one different extension chain, i.e., DNA operator, as control group.

[0042] The atomic force microscope experiment is used to verify the output of different operation results, and it is reflected in the form of Karnaugh map, such as Fig. 9 As shown, it can be seen that the DNA computing architecture can run successfully, Figure 2 The designed operation results show consistent effects.

[0043] In the description of this specification, the description with reference to the terms "one embodiment", "example", "specific example", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0044] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments, and the above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, and these changes and improvements all fall within the scope of the present invention to be protected.

Claims

1. A DNA computing architecture based on dynamic assembly of DNA nanostructures, characterized in that: It includes a DNA central processor, a DNA memory, an input device and an output device; the DNA central processor includes a DNA controller and a DNA operator; The DNA controller is a central DNA tetrahedron nanostructure, and the DNA operator is an edge DNA tetrahedron nanostructure; a central DNA tetrahedron nanostructure and a plurality of edge DNA tetrahedron nanostructures form a DNA tetrahedron polymer nanostructure through complementary pairing of extended chain bases, i.e., a DNA central processor; The DNA memory is an addressable DNA origami in the form of an asymmetric rectangular two-dimensional DNA nanostructure, comprising a DNA template chain and a DNA staple chain; a plurality of capture chains are anchored on the surface of the asymmetric rectangular two-dimensional DNA nanostructure, and are respectively complementary to the extended chains of different edge DNA tetrahedral nanostructures in base pairing; an asymmetric DNA origami replacement chain is arranged on the asymmetric rectangular two-dimensional DNA nanostructure.

2. The DNA computing architecture based on dynamic assembly of DNA nanostructures according to claim 1, characterized in that: The central DNA tetrahedron nanostructure and the edge DNA tetrahedron nanostructure are both composed of four DNA backbone chains, each of which is stacked to form a triangle to form each face of the DNA tetrahedron nanostructure, and each side of the central DNA tetrahedron nanostructure and the edge DNA tetrahedron nanostructure is composed of two DNA chains with complementary base pairing.

3. The DNA computing architecture based on dynamic assembly of DNA nanostructures according to claim 2, characterized in that: In the DNA tetrahedral polymer nanostructure, the number of edge DNA tetrahedral nanostructures is four; the number of capture chains on the surface of the asymmetric rectangular two-dimensional DNA nanostructure is four, and they are respectively complementary to the extended chains of the four edge DNA tetrahedral nanostructures; The four vertices of the central DNA tetrahedron nanostructure are respectively connected to an extended chain, and one of the vertices of the four edge DNA tetrahedron nanostructures is respectively connected to an extended chain, with a total of 8 extended chains; the four extended chains on the central DNA tetrahedron nanostructure are respectively complementary to the extended chains on the four edge DNA tetrahedron nanostructures in base pairing.

4. The DNA computing architecture based on dynamic assembly of DNA nanostructures according to claim 3, characterized in that: The sequences of the four DNA backbone chains are shown as: SEQ ID NO.1-SEQ ID NO.4; The sequences of the four extended chains on the four edge tetrahedral DNA nanostructures are shown as SEQ ID NO.5 to SEQ ID NO.8 respectively; the sequences of the four extended chains on the central tetrahedral DNA nanostructure are shown as SEQ ID NO.9 to SEQ ID NO.12 respectively.

5. The DNA computing architecture based on dynamic assembly of DNA nanostructures according to claim 2, characterized in that: The DNA template strand is the M13mp18 phage genomic DNA sequence; the M13mp18 phage genomic DNA sequence is shown as SEQ ID NO.

13.

6. The DNA computing architecture based on dynamic assembly of DNA nanostructures according to claim 3, characterized in that: The nucleotide sequences of the four capture chains are shown in SEQ ID NO.32 to SEQ ID NO.35 respectively.

7. The DNA computing architecture based on dynamic assembly of DNA nanostructures according to claim 2, characterized in that: The nucleotide sequences of the asymmetric DNA origami replacement chains are shown in SEQ ID NO.14 to SEQ ID NO.

31.

8. The method for preparing a DNA computing architecture based on dynamic assembly of DNA nanostructures according to any one of claims 2 to 7, characterized in that: The following steps are involved: S1, the DNA template strand, DNA staple strand, asymmetric DNA origami replacement strand and capture strand are mixed in a buffer solution, and an asymmetric rectangular two-dimensional DNA nanostructure is obtained after annealing and ultrafiltration purification; S2, mixing the DNA backbone chains in a buffer, annealing, and ultrafiltration purification to obtain central DNA tetrahedral nanostructures and edge DNA tetrahedral nanostructures with different extended chains; S3, mixing the central DNA tetrahedral nanostructure and the edge DNA tetrahedral nanostructure in a buffer solution, and annealing to obtain a DNA tetrahedral polymer nanostructure.

9. The method for preparing a DNA computing architecture based on dynamic assembly of DNA nanostructures according to claim 8, characterized in that: In S1, the buffer is 1×TAE / Mg 2+ buffer, in the mixed solution, the concentration of the DNA template strand was 10 nM, and the total concentration of the DNA staple strand, the asymmetric DNA origami replacement strand, and the capture strand was 80 nM; The buffer in S2 and S3 is TM buffer.

10. Application of the DNA computing architecture based on dynamic assembly of DNA nanostructures according to any one of claims 1 to 7 in miRNA detection.

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