Rapid agilawood formation method for dalbergia bentonii

Abstract

The invention provides a rapid agilawood formation method for dalbergia bentonii, which comprises the following steps of: selecting a dalbergia bentonii plant, and drilling a hole in a trunk along the horizontal direction or in a downward inclined manner; after the holes are drilled, the edgeworthia chrysantha liquid fermented twice is injected into each hole. The edgeworthia chrysantha liquid comprises the following raw materials: rice, eclipta, red-knees herb and pandan leaves. Mixing and crushing edgeworthia chrysantha liquid raw materials, and adding water for fermentation to obtain a fermented primary mixed solution; and filtering the fermentation primary mixed liquid, carrying out fungal fermentation again after filtering, and filtering again to obtain the edgeworthia chrysantha liquid. According to the rapid edgeworthia chrysantha prain ex benth edgeworthia chrysantha prain ex benth edgeworthia chrysantha prain ex benth edgeworthia chrysantha prain ex benth edgeworthia chrysantha prain ex benth edgeworthia chrysantha prain ex benth

Description

Technical Field

[0001] The invention relates to the technical field of incense formation, and in particular to a method for quickly forming incense with Dalbergia odorifera. Background Art

[0002] Dalbergia benthamii Prain is a vine of the genus Dalbergia in the Fabaceae family. The name "Dalbergia benthamii Prain" was first recorded in "Illustrated Description of Major Plants in China - Fabaceae". It is mainly distributed in Guangdong, Hainan, Guangxi and other places in China. Dalbergia benthamii Prain (large leaf) is a perennial woody vine plant. After being injured, it secretes oil to repair the wound and produces a fragrance called agarwood, also known as purple vine incense and chicken bone incense. It usually takes more than 50 years for the agarwood produced in Hainan to produce fragrance. The oil of agarwood is mainly concentrated in the injured part. Generally, the forked part of the vine, the injured and infected part, and the wound part are easy to accumulate oil, and the oil is rich and the fragrance is clear. Agarwood contains rich flavonoids and has multiple biological activities. It can relieve pain, stop bleeding, resist bacteria, and reduce inflammation. Its unique fragrance and medicinal value are deeply loved by consumers. At present, the methods for rapid formation of Dalbergia odorifera mainly include: bacterial liquid infusion method, cutting method, drilling method, fire burning method, etc., but there are the following problems: first, the period of natural formation and artificial wounding technology is very long, and natural formation generally takes more than 50 years; second, the quality of agarwood obtained by traditional methods is uneven, which is difficult to meet the market demand for high-quality agarwood; third, unreasonable artificial wounding technology will cause irreversible damage to Dalbergia odorifera plants. Therefore, the present invention provides a method for rapid formation of Dalbergia odorifera. Summary of the invention

[0003] In view of this, the present invention develops a method for quickly forming fragrance of Dalbergia odorifera.

[0004] The technical solution of the present invention is achieved in this way:

[0005] A method for quickly forming incense from Dalbergia odorifera, comprising the following steps:

[0006] S1, select Dalbergia odorifera plants and drill holes horizontally or downwardly on the trunk;

[0007] S2, after drilling, injecting fermented aroma liquid into each hole, the aroma liquid raw materials including: 2-3 parts of rice, 1-2 parts of Eclipta prostrata, 1-1.5 parts of Polygonum hydropiper, and 0.8-1.2 parts of Pandan leaves.

[0008] The preparation method of the aroma liquid comprises: mixing aroma liquid raw materials, crushing, adding water for the first time to ferment to obtain a fermentation mixed liquid; filtering the fermentation mixed liquid, performing a second fungal fermentation after filtration, and filtering again to obtain the aroma liquid.

[0009] Preferably, the water-added fermentation is anaerobic fermentation, the fermentation time is 38-42 days, and the fermentation temperature is 28-32°C.

[0010] Preferably, the fermentation initial mixed solution obtained by the first water-adding fermentation consists of rice, Eclipta prostrata, Polygonum hydropiper, Pandan leaves and water in a mass volume ratio of (2-3) g: (1-2) g: (1-1.5) g: (0.8-1.2) g: (25-35) mL.

[0011] Preferably, the fungi are Trichoderma atroviride and Trichoderma harziauum, and the mass ratio of the fermentation initial mixed liquid, Trichoderma atroviride and Trichoderma harziauum is (100-90):(3-6):(2-5).

[0012] Preferably, the fungus fermentation has a fermentation temperature of 24-26° C., a fermentation time of 6-7 days, a pH value of 5-5.2, and a sealed anaerobic fermentation condition.

[0013] Preferably, S1, for the drilling, select Dalbergia dasyphylla plants with a height of 18-22 cm and a trunk diameter > 5 cm, and use a 1.7-2.0 mm diameter drill bit to drill holes in parallel at a height of 20-30 cm from the ground along the horizontal direction or horizontally downward at an angle of 3-6° to penetrate the trunk. The horizontal distance between adjacent holes is 1.7-2.0 cm, and the distance between adjacent upper and lower rows is 15-20 cm.

[0014] Preferably, S2, the injection of fragrance liquid, injecting 0.2-0.25g of fragrance liquid into each hole once within 25-30 minutes after drilling.

[0015] Compared with the prior art, the present invention has the following beneficial effects:

[0016] The invention selects Dalbergia odorifera plants from Guangdong and Guangdong, drills holes on the trunk, and injects twice-fermented aroma liquid into each hole. The aroma liquid raw materials are compounded with rice, Eclipta prostrata, Polygonum hydropiper and Pandan leaves, and the compound aroma liquid raw materials improve the aroma production efficiency and the extract content; the aroma liquid is anaerobic fermented by the second fermentation process, the raw materials are mixed, crushed, and water is added for fermentation to obtain the fermentation primary mixed liquid, which is filtered and then fermented by fungi again, and the aroma liquid finished product is filtered. The method for quickly forming fragrance of Dalbergia odorifera using the method of the present invention can form fragrance again 1-1.2 years after the fragrance is formed, and the types and contents of volatile substances in the extract are increased. The volatile oil includes elemenine, methyl eugenol, whiskey lactone, isoelemenine, linalool and cis-cubane, among which whiskey lactone, linalool and cis-B-cubane are detected, among which elemenine accounts for 73%-89%, methyl eugenol accounts for 7.6%-20.0%, and also includes whiskey lactone, isoelemenine, linalool and cis-B-cubane. In addition, a total of 8 flavor components are detected, all of which contain methyl eugenol and isoelemenine. In the fragrance products formed by the method for quickly forming fragrance of Dalbergia odorifera using the method of Examples 1-3 of the present invention, the types of volatile oils are rich and the content is increased. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 : After the incense treatment, obvious oil accumulation appears in the wood of the trunk, and as the years increase, black oil will cover the entire trunk. The black oil is the incense of Dalbergia odorifera, also known as agarwood, which can be used as medicine, extract essential oils or make aromatic products. The black oil in the picture is intermittently distributed because the oil is first formed at the incense hole and gradually extends along the trunk. The longer the time, the longer the extension, and finally the entire trunk is almost full of oil.

[0018] Figure 2 : The trunks of plants that have not been treated with fragrance are light in color and have no oil accumulation. DETAILED DESCRIPTION

[0019] In order to better understand the technical content of the present invention, specific examples are provided below to further illustrate the present invention. The experimental methods used in the examples of the present invention are all conventional methods unless otherwise specified.

[0020] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial sources.

[0021] Trichoderma atroviride: The strain comes from the Institute of Microbiology, Chinese Academy of Sciences, original number E115, effective viable count ≥ 12 billion / g; Trichoderma harziauum: The strain comes from the Chinese Microbial Culture Center, effective viable count ≥ 2.5 billion / g.

[0022] Preparation Example 1

[0023] The preparation method of the fragrance liquid comprises: mixing 2 parts of rice, 1 part of Ecliptae, 1 part of Polygonum hydropiper, and 1 part of Pandan leaves as raw materials of the fragrance liquid, crushing, adding water for anaerobic fermentation, fermentation time for 38 days, fermentation temperature for 28°C, obtaining a fermentation initial mixed liquid, wherein the mass volume ratio of rice, Ecliptae, Polygonum hydropiper, Pandan leaves and water in the fermentation initial mixed liquid is 2g:1g:1g:1g:25mL; filtering the fermentation initial mixed liquid, and then performing fungal fermentation again after filtration, wherein the fungi are Trichoderma atroviride and Trichoderma harziauum, wherein the mass ratio of the fermentation initial mixed liquid, Trichoderma atroviride and Trichoderma harziauum is 100:3:2, the fermentation temperature of the fungal fermentation is 24°C, the fermentation time is 6 days, the pH value is 5, and the fermentation condition is sealed anaerobic fermentation. Filtering again to obtain the fragrance liquid.

[0024] Preparation Example 2

[0025] The preparation method of the fragrance liquid comprises: mixing 3 parts of rice, 1.2 parts of Ecliptae, 1 part of Polygonum hydropiper, and 1.3 parts of Pandan leaves as raw materials of the fragrance liquid, crushing, adding water for anaerobic fermentation, fermentation time for 42 days, fermentation temperature for 32°C, obtaining a fermentation initial mixed liquid, wherein the mass volume ratio of rice, Ecliptae, Polygonum hydropiper, Pandan leaves and water in the fermentation initial mixed liquid is 3g:1.2g:1.2g:1.2g:35mL; filtering the fermentation initial mixed liquid, and then performing fungal fermentation again after filtration, wherein the fungi are Trichoderma atroviride and Trichoderma harziauum, wherein the mass ratio of the fermentation initial mixed liquid, Trichoderma atroviride and Trichoderma harziauum is 90:6:5, the fermentation temperature of the fungal fermentation is 26°C, the fermentation time is 7 days, the pH value is 5.2, and the fermentation condition is sealed anaerobic fermentation. Filtering again to obtain the fragrance liquid.

[0026] Preparation Example 3

[0027] The preparation method of the fragrance liquid comprises: mixing 2.5 parts of rice, 1.1 parts of Ecliptae, 1.1 parts of Polygonum hydropiper, and 1 part of Pandan leaves as raw materials of the fragrance liquid, crushing, adding water for anaerobic fermentation, fermentation time for 40 days, fermentation temperature for 30°C, obtaining a fermentation initial mixed liquid, wherein the mass volume ratio of rice, Ecliptae, Polygonum hydropiper, Pandan leaves and water in the fermentation initial mixed liquid is 2.5g:1.1g:1.1g:1g:28mL; filtering the fermentation initial mixed liquid, and then performing fungal fermentation again after filtration, wherein the fungi are Trichoderma atroviride and Trichoderma harziauum, wherein the mass ratio of the fermentation initial mixed liquid, Trichoderma atroviride and Trichoderma harziauum is (100-90):(3-6):(2-5), the fermentation temperature of the fungal fermentation is 25°C, the fermentation time is 6.5 days, the pH value is 5.1, and the fermentation condition is sealed anaerobic fermentation. Filtering again to obtain the fragrance liquid.

[0028] Example 1

[0029] A method for quickly forming incense from Dalbergia odorifera, comprising the following steps:

[0030] Step 1: Select a Dalbergia odorifera plant with a height of 18 cm and a trunk diameter of 5.5 cm. At a height of 22 cm from the ground, use a 1.8 mm diameter drill bit to drill holes side by side horizontally or 3° downward on the trunk of the plant, penetrating the trunk. The horizontal distance between adjacent holes is 1.7 cm, and the distance between adjacent upper and lower rows is 15 cm.

[0031] Step 2: Within 25 minutes after drilling, inject 0.2 g of incense-forming liquid into each hole at one time. The incense-forming liquid is prepared according to the method of Preparation Example 1.

[0032] Example 2

[0033] Step 1: Select a Dalbergia odorifera plant with a height of 22 cm and a trunk diameter of 6 cm. At a height of 30 cm from the ground, use a 2.0 mm diameter drill bit to drill holes side by side at 6° horizontally or horizontally downward on the trunk of the plant, penetrating the trunk. The horizontal distance between adjacent holes is 2.0 cm, and the distance between adjacent upper and lower rows is 20 cm.

[0034] Step 2: Within 30 minutes after drilling, inject 0.25 g of incense-forming liquid into each hole at one time. The incense-forming liquid is prepared according to the method of Preparation Example 2.

[0035] Example 3

[0036] Step 1: Select a Dalbergia odorifera plant with a height of 20 cm and a trunk diameter of 5.5 cm. At a height of 25 cm from the ground, use a 1.8 mm diameter drill bit to drill holes in the trunk of the plant horizontally or horizontally downward at 4° side by side, penetrating the trunk. The horizontal distance between adjacent holes is 1.9 cm, and the distance between adjacent upper and lower rows is 18 cm.

[0037] Step 2: Within 28 minutes after drilling, inject 0.23 g of incense-forming liquid into each hole at one time. The incense-forming liquid is prepared according to the method of Preparation Example 3.

[0038] Comparative Example 1

[0039] The main difference between Comparative Example 1 and Example 1 is that the raw materials of rice and Eclipta prostrata for the fragrance-forming liquid are removed from the preparation method of the fragrance-forming liquid in Preparation Example 1, and the other methods and steps are consistent with those in Example 1.

[0040] Comparative Example 2

[0041] The main difference between Comparative Example 2 and Example 1 is that the Polygonum hydropiper and Pandanus leaf are removed from the preparation method of the fragrance liquid in Preparation Example 1, and the other methods and steps are consistent with Example 1.

[0042] Comparative Example 3

[0043] The main difference between Comparative Example 3 and Example 1 is that in the preparation method of the fragrance-forming liquid in Preparation Example 1, the two anaerobic fermentations are changed to aerobic fermentations, and the other methods and steps are consistent with Example 1.

[0044] Comparative Example 4

[0045] The main difference between Comparative Example 4 and Example 1 is that the fungal fermentation step is removed from the preparation method of the fragrance liquid in Preparation Example 1, and the other methods and steps are consistent with Example 1.

[0046] Test 1

[0047] Each example is divided into 3 groups, Example 1 (Example 1-1, Example 1-2, Example 1-3), Example 2 (Example 2-1, Example 2-2, Example 2-3) and Example 3 (Example 3-1, Example 3-2, Example 3-3), and the comparative example grouping method is the same as the example. The 13-month extracts obtained from Examples 1-3 and Comparative Examples 1-4 were steam distilled to extract volatile oil, the mass volume ratio of the extract to water was 1g:5-8mL, the extraction time was 30-50min, the temperature was 20-30°C, and the volatile oil obtained by distillation was dried with anhydrous sodium sulfate and weighed. The volatile oil was subjected to GC-MS analysis and qualitative analysis of chemical composition categories. The experimental results show that the average value of the volatile oil content of Examples 1-3 ranges from 2.48% to 2.56%, and the range of Comparative Examples 1-4 is 0.96% to 1.10%. The volatile oil content of Example 1-3 is significantly higher than that of the comparative example, as shown in Table 1.

[0048] Table 1

[0049] Group Sample type Average volatile oil content (%) Example 1 Raw Materials 2.56a Example 2 Raw Materials 2.48a Example 3 Raw Materials 2.53a Comparative Example 1 Raw Materials 1.02b Comparative Example 2 Raw Materials 0.98b Comparative Example 3 Raw Materials 1.10b Comparative Example 4 Raw Materials 0.96b

[0050] The volatile oil extracted above was subjected to GC-MS determination and analysis. The results showed that a total of 81 components were detected in the volatile oil, of which 24 were sesqui-type compounds. The data of the volatile oil components with higher content are listed in Table 2. The main components in the volatile oil are elemenine, methyl eugenol, whiskey lactone, isoelemenine, linalool and cis-cubane, among which whiskey lactone, linalool and cis-B-cubane were detected, and the relative percentage content ranges of the above main components were 73%-89% (elemenine), 7.6%-20.0% (methyl eugenol), 0.03%-2.51% (whiskey lactone), 0.15-0.79% (isoelemenine), 0.01%-0.55% (linalool) and 0.01%-0.66% (cis-B-cubane); in addition, a total of 8 kinds of fragrance components were detected, all of which contained methyl eugenol and isoelemenine, as shown in Table 2 for details. It is explained that the volatile oil products produced by the rapid method for producing Dalbergia paniculata in accordance with the methods of Examples 1-3 are rich in variety and increased in content.

[0051] Table 2

[0052]

[0053]

[0054] Test 2:

[0055] Experiment on xylene-induced ear swelling in mice. 45 mice were randomly divided into a blank group, a dexamethasone positive control group, an example 1-3 Dalbergia chinensis extract group and a comparative example 1-4 Dalbergia chinensis extract group, with 5 equilibrium tests in each group. Except for the blank group, which was gavaged with distilled water, the other groups were given corresponding doses of drugs, once a day, with each dose of 15 mL / kg, for 7 consecutive days. Fasting but not water control for 12 hours before the last administration, 60 minutes after administration, 0.02 mL of xylene was dripped into the right ear of each group of mice to cause inflammation, and death was caused 15 minutes later. The two ears of the inflamed mice were punched with a 5 mm diameter puncher, weighed, and the swelling degree and swelling inhibition rate of the mouse ears were calculated.

[0056] Ear swelling (mg) = right ear piece weight - left ear piece weight

[0057] The Dalbergia chinensis extract group of Example 1-3 effectively inhibited the swelling of the mouse auricle, and the inhibitory effect was better than that of the comparative example and the control group, indicating that the Dalbergia chinensis extract obtained by the raw materials and methods of the present invention has good anti-inflammatory activity and has an anti-inflammatory effect on the ear.

[0058] Table 3

[0059]

[0060]

[0061] Test results: The raw materials of the fragrance liquid of the present invention are rice, Eclipta prostrata, Polygonum hydropiper and Pandan leaves, and the fragrance liquid is fermented anaerobically by a two-time fermentation process. The raw materials are mixed, crushed, and fermented with water to obtain the fermentation initial mixed liquid, which is filtered and then fermented again by fungi to obtain the fragrance liquid product. Select Dalbergia chinensis plants, drill holes on the trunk, and inject the fragrance liquid after the second fermentation into each hole to form fragrance. The method for rapid fragrance formation of Dalbergia chinensis using the method of the present invention can form fragrance again 1-1.2 years after the fragrance is formed, which improves the fragrance production efficiency and extract content of Dalbergia chinensis. The fragrance liquid is prepared by mixing rice, Eclipta prostrata, Polygonum hydropiper and Pandan leaves to increase the types and contents of volatiles in the extract, and the combined use of the two-time fermentation process improves the antibacterial ability and anti-inflammatory activity of the extract. When the fragrance liquid raw materials are changed in Comparative Examples 1-2 and the fermentation process is changed in Comparative Examples 3-4, the antibacterial effect in the extract is significantly reduced. The raw materials, raw material ratio and method of the aroma liquid of the present invention can make Dalbergia odorifera quickly form aroma, so that the volatile oil includes flavor components such as elemenine, methyl eugenol, and whiskey lactone. In the aroma product of the Dalbergia odorifera quick aroma formation method according to the methods of Examples 1-3 of the present invention, the variety and content of volatile oil are improved.

[0062] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for quickly forming fragrance of Dalbergia odorifera, characterized in that: The following steps are involved: S1, select Dalbergia odorifera plants and drill holes horizontally or downwardly on the trunk; S2, after drilling, inject the fermented aroma liquid into each hole; The raw materials of the fragrance-forming liquid include: 2-3 parts of rice, 1-2 parts of Eclipta prostrata, 1-1.5 parts of Polygonum hydropiper, and 0.8-1.2 parts of Pandan leaves.

2. A method for rapidly forming fragrance of Dalbergia odorifera according to claim 1, characterized in that: The preparation method of the aroma liquid comprises: mixing aroma liquid raw materials, crushing, adding water for the first time to ferment to obtain a fermentation mixed liquid; filtering the fermentation mixed liquid, performing a second fungal fermentation after filtering, and filtering again to obtain the aroma liquid.

3. A method for rapidly forming fragrance of Dalbergia odorifera according to claim 2, characterized in that: The first water-adding fermentation is anaerobic fermentation, with a fermentation time of 38-42 days and a fermentation temperature of 28-32°C.

4. The method for rapidly forming fragrance of Dalbergia odorifera according to claim 1, characterized in that: The fermentation initial mixed solution obtained by the first water addition fermentation is composed of rice, Eclipta prostrata, Polygonum hydropiper, Pandan leaves and water in a mass volume ratio of (2-3) g: (1-2) g: (1-1.5) g: (0.8-1.2) g: (25-35) mL.

5. The method for rapidly forming fragrance of Dalbergia odorifera according to claim 2, characterized in that: The fungi are Trichoderma atroviride and Trichoderma harziauum, and the mass ratio of the fermentation initial mixed liquid, Trichoderma atroviride and Trichoderma harziauum is (100-90):(3-6):(2-5).

6. The method for rapidly forming fragrance of Dalbergia odorifera according to claim 2, characterized in that: The fungus fermentation has a fermentation temperature of 24-26° C., a fermentation time of 6-7 days, a pH value of 5-5.2, and a sealed anaerobic fermentation condition.

7. The method for rapidly forming fragrance of Dalbergia odorifera according to claim 1, characterized in that: S1, for drilling, select Dalbergia chinensis plants, use a 1.7-2.0 mm diameter drill bit to drill holes in parallel at a height of 20-30 cm from the ground along the horizontal direction or horizontally downward at an angle of 3-6° on the trunk of the plant, penetrate the trunk, the horizontal distance between adjacent holes is 1.7-2.0 cm, and the distance between adjacent upper and lower rows is 15-20 cm.

8. The method for rapidly forming fragrance of Dalbergia odorifera according to claim 1, characterized in that: S1, the Dalbergia chinensis plant has a plant height of 18-22 cm and a trunk diameter of >5 cm.

9. The method for rapidly forming fragrance of Dalbergia odorifera according to claim 1, characterized in that: S2, the fragrance liquid is injected, and 0.2-0.25g of fragrance liquid is injected into each hole at one time within 25-30 minutes after drilling.

10. The method for rapidly forming fragrance of Dalbergia odorifera according to claim 1, characterized in that: After producing fragrance in the first year, it will produce fragrance again after 1-1.2 years.

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