Traditional Chinese medicine composition with effects of reducing oxalic acid in urine and increasing citric acid in urine and application of traditional Chinese medicine composition

Abstract

The invention discloses a traditional Chinese medicine composition with the effects of reducing urological oxalic acid and increasing urological citric acid and application thereof.The traditional Chinese medicine composition is prepared from eight main raw medicinal materials and minor raw medicinal materials, and the main raw medicinal materials comprise lalang grass rhizome, microcos paniculata, wild buckwheat, chrysanthemum, folium mori, sophora flower, lophatherum gracile and liquorice; the secondary raw medicinal materials comprise rheum officinale, catechu, caulis spatholobi, fructus lycii, fructus canarii, folium apocyni veneti, rhizoma smilacis glabrae, kiwi fruit, cortex cinnamomi, kapok, radix ampelopsis, sanguisorba officinalis, fructus xanthii, rhizoma galangae, flos carthami and herba cepbalanoplosis segeti. The traditional Chinese medicine composition has good effects of reducing urinary oxalic acid and increasing urinary citric acid, the effect is better than that of potassium sodium hydrogen citrate in the aspect of increasing urinary citric acid, and the traditional Chinese medicine composition adopts medicinal and edible raw medicinal materials in China, has the characteristics of safe components and no side effect, does not cause the side effect of potassium citrate, and is suitable for large-scale production. The compound can be used for preparing food, medicines and health-care products with the effects of reducing urological oxalic acid and increasing urological citric acid.

Description

Technical Field

[0001] The invention belongs to the field of medical care and health, and specifically relates to a traditional Chinese medicine composition having the effects of reducing urine oxalic acid and increasing urine citric acid and an application thereof. Background Art

[0002] Kidney stones are a common urinary system disease, which are mineral deposits formed in the kidneys due to urine salt supersaturation. Urinary stones, including renal pelvic stones, ureteral stones, bladder stones and urethral stones, mostly originate from the kidneys, with an incidence rate of 5-12% and a recurrence rate of up to 50% in 5 years, which seriously affects human health. The composition of kidney stones varies, calcium oxalate stones are the most common, accounting for about 80%, uric acid stones account for about 10%, followed by struvite stones (5%), calcium phosphate stones (1%) and cystine stones (1%). Kidney stones are a complex metabolic disease, among which hyperoxaluria and hypocitrateuria are high-risk factors affecting urine salt supersaturation and the occurrence and development of kidney stones.

[0003] Abnormalities in the human body's oxalic acid metabolism process lead to increased oxalic acid absorption, synthesis, and excretion, which can cause severe hyperoxaluria, combining with free calcium ions in the body to form crystals in the renal tubules and renal interstitium of the kidney. Oxalic acid enters the body mainly through intestinal absorption and liver synthesis, and is excreted through the kidneys as a metabolic end product, so the kidneys are susceptible to oxalic acid. Calcium oxalate is deposited in the kidneys, which can easily cause stones, renal calcinosis, early renal failure, etc. At present, there are few products developed in the market for reducing urinary oxalic acid. There is an "enzyme fossil" product that reduces intestinal absorption of oxalic acid by regulating intestinal flora to decompose intestinal oxalic acid, and its effect needs further verification. In addition, a drug used to treat childhood epilepsy, Stripentol, can reduce the excretion of urinary oxalic acid to a certain extent, but Stripentol has serious side effects, including drowsiness, decreased appetite, weight loss, neutropenia, thrombocytopenia, and suicidal thoughts or behaviors.

[0004] On the other hand, there are factors that inhibit crystallization / stone formation during the formation of kidney stones. These inhibitors can increase the threshold of urine supersaturation, reduce crystal aggregation and / or adhesion to renal tubular epithelial cells, and the most important crystallization inhibitor is urinary citrate. The content of citrate in normal human urine is 3-4 molar concentration, which can effectively inhibit the growth and aggregation of calcium oxalate crystals in urine. According to surveys, about 19-63% of patients with calcium stones are accompanied by hypocitraturia, so hypocitraturia is one of the important risk factors for the formation of calcium oxalate stones. Potassium citrate, also known as tripotassium citrate, is a potent crystal inhibitor used for the short-term treatment of renal tubular acidosis, hypocitraturian calcium oxalate stones and uric acid stones. Potassium citrate has a significant therapeutic effect on patients with renal tubular acidosis and stones caused by hypocitraturian calcium oxalate, but long-term use may cause the following side effects: (1) Gastrointestinal discomfort: Intake of more potassium citrate is prone to nausea, vomiting, chest pain, and abdominal pain. In severe cases, gastrointestinal ulcers and bleeding may also occur, especially in patients with primary gastrointestinal diseases; (2) Effects on the nervous system: If a large amount of this substance is ingested, the intake of more potassium ions may cause instability of the child's nervous system, headaches and excitement, which has a great impact on the healthy growth of children; (3) Hyperkalemia: If potassium citrate is not taken properly, it leads to excessive intake of potassium ions, which may cause hyperkalemia and fatigue; (4) Cardiac arrest: Studies have shown that large amounts of potassium citrate can cause cardiac arrest. Therefore, effectively increasing urinary citrate is important for treating kidney stones and preventing their recurrence. Summary of the invention

[0005] In view of the current reality of a lack of products that can lower urine oxalate and increase urine citrate, the purpose of the present invention is to utilize my country's abundant traditional Chinese medicine resources to provide a traditional Chinese medicine composition with the effects of lowering urine oxalate and increasing urine citrate, as well as a preparation method and application thereof, for the treatment of kidney stones and the prevention of recurrence of kidney stones.

[0006] The objective of the present invention is achieved through the following technical solutions:

[0007] The present invention provides a Chinese medicine composition having the effects of reducing urine oxalic acid and increasing urine citric acid, comprising 8 main raw medicinal material components and secondary raw medicinal material components;

[0008] Among them, the 8 main raw medicinal materials are: Imperata root, Buza leaf, Fagopyrum sibiricum, Chrysanthemum, Mulberry leaf, Sophora japonica flower, Lophatherum gracile, and Licorice; the secondary raw medicinal materials include: Rhubarb, Catechu, Millettia reticulata, Lycium barbarum, Green fruit, Lophatherum venetum leaf, Smilax glabra, Kiwi fruit, Cinnamon bark, Kapok flower, Bletilla striata, Sanguisorba officinalis, Xanthium sibiricum, Alpinia officinalis, Safflower, and Thistle.

[0009] Preferably, the Chinese medicine composition having the effects of lowering urinary oxalate and raising urinary citric acid is composed of the 8 main raw medicinal ingredients and any 1-4 secondary raw medicinal ingredients.

[0010] Further preferably, the Chinese medicine composition having the effects of lowering urinary oxalate and raising urinary citric acid is composed of the above 8 main raw medicinal ingredients.

[0011] The Chinese medicine composition with the effects of lowering urine oxalate and raising urine citric acid, wherein the main raw medicinal materials are composed of the following weight ratios: 20-200 parts by weight of Imperata root, 20-200 parts by weight of Buza leaf, 60-600 parts by weight of Fagopyrum truncatum, 40-400 parts by weight of Chrysanthemum, 20-200 parts by weight of Mulberry leaf, 10-200 parts by weight of Sophora japonica flower, 20-200 parts by weight of Lophatherum gracile leaf, and 20-200 parts by weight of Licorice;

[0012] The secondary raw medicinal material components are composed of the following weight proportions: 20-500 weight parts of rhubarb, 20-200 weight parts of catechu, 80-500 weight parts of Millettia spatholobi, 300-700 weight parts of wolfberry, 20-100 weight parts of green fruit, 20-200 weight parts of Apocynum venetum leaves, 20-400 weight parts of Smilax glabra, 100-800 weight parts of kiwi fruit, 10-100 weight parts of cinnamon bark, 20-100 weight parts of kapok flowers, 20-200 weight parts of white ampel, 70-200 weight parts of sanguisorba officinalis, 20-100 weight parts of Xanthium sibiricum, 400-800 weight parts of galangal, 100-500 weight parts of safflower, and 100-500 weight parts of thistle.

[0013] Preferably, the Chinese medicine composition having the effects of lowering urine oxalate and raising urine citric acid of the present invention is composed of the following medicinal materials in weight ratio:

[0014] The proportion of 8 main medicinal materials: 20-100 parts by weight of Imperata root, 60-100 parts by weight of Buza leaf, 100-400 parts by weight of Golden Buckwheat, 100-200 parts by weight of Chrysanthemum, 20-100 parts by weight of Mulberry leaf, 60-120 parts by weight of Sophora japonica flower, 20-100 parts by weight of Lophatherum gracile leaf, and 20-100 parts by weight of Licorice root;

[0015] The weight ratio of the secondary raw medicinal materials is as follows: 100-300 parts by weight of rhubarb, 100-200 parts by weight of catechu, 100-300 parts by weight of Millettia spatholobi, 400-600 parts by weight of wolfberry, 40-80 parts by weight of green fruit, 40-100 parts by weight of dogbane leaves, 40-300 parts by weight of smilax glabra, 200-600 parts by weight of kiwi fruit, 20-80 parts by weight of cinnamon bark, 40-80 parts by weight of kapok, 50-100 parts by weight of white ampel, 90-150 parts by weight of sanguisorba officinalis, 40-80 parts by weight of Xanthium sibiricum, 650-750 parts by weight of galangal, 200-400 parts by weight of safflower, and 200-400 parts by weight of thistle.

[0016] Further preferably, the Chinese medicine composition having the effects of lowering urine oxalate and raising urine citrate of the present invention is composed of the following medicinal materials in weight ratio:

[0017] The proportion of 8 main raw materials is: 20-50 parts by weight of Imperata root, 80-100 parts by weight of Buza leaf, 200-400 parts by weight of Golden Buckwheat, 150-200 parts by weight of Chrysanthemum, 20-60 parts by weight of Mulberry leaf, 80-100 parts by weight of Sophora japonica flower, 20-50 parts by weight of Lophatherum gracile leaf, and 20-50 parts by weight of Licorice root.

[0018] The weight ratio of secondary raw medicinal materials: 180-240 weight parts of rhubarb, 120-180 weight parts of catechu, 150-250 weight parts of Millettia spatholobi, 450-550 weight parts of wolfberry, 50-70 weight parts of green fruit, 60-80 weight parts of dogbane leaves, 50-200 weight parts of smilax glabra, 300-500 weight parts of kiwi fruit, 30-60 weight parts of cinnamon bark, 50-70 weight parts of kapok, 60-80 weight parts of white ampel, 100-130 weight parts of sanguisorba officinalis, 50-70 weight parts of Xanthium sibiricum, 680-720 weight parts of galangal, 280-340 weight parts of safflower, and 280-340 weight parts of thistle.

[0019] Finally, the preferred Chinese medicine composition of the present invention having the effects of lowering urine oxalate and raising urine citrate is composed of the following medicinal materials in weight ratio:

[0020] 100 parts by weight of Imperata rhizome, 50 parts by weight of Buza leaves, 200 parts by weight of Fagopyrum truncatum, 150 parts by weight of chrysanthemum, 100 parts by weight of mulberry leaves, 100 parts by weight of Sophora japonica flower, 100 parts by weight of Lophatherum gracile, and 100 parts by weight of Licorice.

[0021] The weight of the above components is calculated based on crude drugs. Each portion can be in grams, kilograms or tons. If grams are used as the unit, the formula composition can be made into 1000 grams of pharmaceutical preparations.

[0022] The preparation method of the Chinese medicine composition having the effects of lowering urine oxalic acid and raising urine citric acid of the present invention comprises the following steps:

[0023] (1) The Chinese medicine composition consists of eight main raw medicinal materials and secondary raw medicinal materials, and the specific formula is clearly defined;

[0024] (2) Weighing the raw Chinese medicine materials according to the weight ratio of the Chinese medicine composition;

[0025] (3) crushing the raw medicinal materials, sieving those with mesh sizes smaller than 18, and mixing;

[0026] (4) Add 500-1000 mL of water each time and boil for 20-30 minutes;

[0027] (5) It can be consumed after it cools down.

[0028] The present invention also provides the use of the traditional Chinese medicine composition in preparing products with the efficacy of reducing urine oxalic acid and increasing urine citric acid, and the products include food, medicine and health care products.

[0029] The Chinese medicine composition is used in the preparation of medicines or health products with the effects of reducing urine oxalate and increasing urine citrate, wherein the medicines also include pharmaceutically acceptable carriers and / or excipients; the dosage form of the medicines or health products is tablets, powders, granules, capsules, emulsions or suspensions.

[0030] The Chinese medicine composition is used in preparing food with the effects of reducing urine oxalic acid and increasing urine citric acid, and the food is at least one of tea bags, health tea, solid food, and liquid beverage.

[0031] Advantages and effects of the present invention:

[0032] The present invention firstly finds that a Chinese medicine composition has good effects of reducing urine oxalic acid and increasing urine citrate, and the effect of increasing urine citrate is better than that of potassium sodium hydrogen citrate;

[0033] The main raw medicinal material components of the invention adopt Chinese raw medicinal materials that are both medicine and food, and have the characteristics of safe components and no side effects, and will not cause the side effects of potassium citrate; and can be used to prepare foods, medicines and health products with the effects of lowering urine oxalate and raising urine citrate. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 Application Example 1 Histogram of the change of oxalic acid in rat urine over time;

[0035] Figure 2 Application Example 1 Histogram of the change of citric acid in rat urine over time;

[0036] Figure 3 Application Example 1: A bar graph showing the effect of Chinese herbal formula combination tea and potassium sodium hydrogen citrate on increasing citric acid over time. DETAILED DESCRIPTION

[0037] The present invention is further described in detail below in conjunction with specific implementation modes.

[0038] Example 1 A Chinese medicine composition having the effect of lowering urine oxalate and raising urine citric acid

[0039] The Chinese medicine composition of this embodiment is composed of 8 main raw materials in the following weight ratio:

[0040] 100 parts by weight of Imperata rhizome, 50 parts by weight of Buza leaves, 200 parts by weight of Fagopyrum truncatum, 150 parts by weight of chrysanthemum, 100 parts by weight of mulberry leaves, 100 parts by weight of Sophora japonica flower, 100 parts by weight of Lophatherum gracile, and 100 parts by weight of Licorice.

[0041] Example 2 A Chinese medicine composition having the effects of lowering urine oxalate and raising urine citric acid

[0042] The Chinese medicine composition of this embodiment is composed of 8 main raw medicinal materials and 2 secondary raw medicinal materials in the following weight ratio:

[0043] 100 parts by weight of Imperata rhizome, 50 parts by weight of Buza leaf, 200 parts by weight of Fagopyrum truncatum, 100 parts by weight of Chrysanthemum, 50 parts by weight of Mulberry leaf, 50 parts by weight of Sophora japonica flower, 100 parts by weight of Lophatherum gracile, 100 parts by weight of Licorice root, 300 parts by weight of Lycium barbarum, and 200 parts by weight of Smilax glabra.

[0044] Example 3 A Chinese medicine composition having the effect of lowering urine oxalate and raising urine citric acid

[0045] The Chinese medicine composition of this embodiment is composed of 8 main raw medicinal materials and 2 secondary raw medicinal materials in the following weight ratio:

[0046] 50 parts by weight of Imperata rhizome, 200 parts by weight of Buza leaves, 200 parts by weight of golden buckwheat, 200 parts by weight of chrysanthemum, 50 parts by weight of mulberry leaves, 50 parts by weight of Sophora japonica flowers, 50 parts by weight of loblolly leaves, 50 parts by weight of liquorice, 50 parts by weight of cinnamon bark, and 50 parts by weight of green fruit.

[0047] Example 4 A Chinese medicine composition having the effect of lowering urine oxalate and raising urine citric acid

[0048] The Chinese medicine composition of this embodiment is composed of 8 main raw medicinal materials and 2 secondary raw medicinal materials in the following weight ratio:

[0049] 50 parts by weight of Imperata rhizome, 100 parts by weight of Buza leaves, 400 parts by weight of golden buckwheat, 200 parts by weight of chrysanthemum, 50 parts by weight of mulberry leaves, 100 parts by weight of Sophora japonica flowers, 50 parts by weight of Lophatherum gracile, 25 parts by weight of liquorice, 50 parts by weight of catechu, and 100 parts by weight of safflower. Add purified water and boil the above ten medicinal materials.

[0050] Example 5 A Chinese medicine composition having the effect of lowering urine oxalate and raising urine citric acid

[0051] The Chinese medicine composition of this embodiment is composed of 8 main raw medicinal materials and 4 secondary raw medicinal materials in the following weight ratio:

[0052] The composition includes 50 parts by weight of Imperata rhizome, 50 parts by weight of Buza leaf, 250 parts by weight of Fagopyrum tiliaceum, 100 parts by weight of chrysanthemum, 50 parts by weight of mulberry leaf, 50 parts by weight of Sophora japonica flower, 50 parts by weight of Lophatherum gracile, 50 parts by weight of Licorice root, 50 parts by weight of Apocynum venetum leaf, 100 parts by weight of kiwi fruit, 20 parts by weight of Kapok flower and 100 parts by weight of Xanthium sibiricum fruit.

[0053] Example 6 Preparation method of a Chinese medicine composition having the effects of lowering urine oxalate and raising urine citric acid

[0054] The Chinese medicine composition of Examples 1-5 is prepared by weighing raw medicinal materials according to the weight ratio of the formula, crushing the raw medicinal materials, sieving them to less than 18 mesh, and then mixing them. 500-1000 mL of pure water is added each time, and the mixture is boiled for 20 minutes and cooled.

[0055] The embodiments of the present invention may arbitrarily combine the various technical features of the invention content. To make the description concise, not all possible combinations of the various technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of the present invention.

[0056] The above-mentioned embodiments only express several implementation methods of the present invention, and the description is relatively specific and detailed, but it cannot be understood as limiting the scope of the invention patent. It should be pointed out that for ordinary technicians in this field, several modifications and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention.

[0057] Application Example 1 Application of a Chinese medicine composition having the effects of lowering urine oxalic acid and raising urine citric acid

[0058] In vivo screening test of the Chinese medicinal composition with the efficacy of lowering urine oxalate and increasing urine citrate

[0059] 1. Experimental purpose: To evaluate the effect of a Chinese herbal medicine composition on lowering oxalate and increasing citric acid at the animal level.

[0060] 2. Experimental Materials:

[0061] 1. Test drugs: The Chinese medicine composition of Example 1 of the present invention, consisting of Imperata root, Buza leaf, Fagopyrum truncatum, Chrysanthemum, Mulberry leaf, Sophora japonica flower, Lophatherum gracile, and Licorice, all of which were purchased from Anhui Bozhou Wenjingtang Biotechnology Co., Ltd.; the positive control drug potassium sodium hydrogen citrate was purchased from Wuhan Viao Pharmaceutical Co., Ltd.

[0062] 2. Animals: 18 male SD rats aged 6-8 weeks were purchased from Liaoning Changsheng Biotechnology Co., Ltd.

[0063] 3. List of reagents, consumables and main instruments:

[0064] Table 1 List of commonly used reagents and consumables

[0065] Reagent name Reagent Company Oxalic acid content detection kit Beijing Solable Technology Co., Ltd. Citric Acid Content Detection Kit Beijing Solable Technology Co., Ltd. 15mL centrifuge tube Beijing Dingguo 1.5mL centrifuge tube Beijing Dingguo 96-well plate Beijing Dingguo SC-3610 low speed centrifuge Anhui Zhongke Zhongjia Scientific Instrument Co., Ltd. ELISA reader Thermo Fully automatic ice machine Shanghai Bilang Instrument Co., Ltd. -80℃ Ultra Low Temperature Refrigerator SANYO -20℃ Refrigerator Zhongke Meiling

[0066] 3. Experimental methods:

[0067] 1. Grouping: The rats were divided into a blank control group, an oxalic acid model group and a combination tea group; the combination tea was made by weighing the medicinal materials in Example 1 according to the ratio to make a tea bag, consisting of 100 parts by weight of Imperata root, 50 parts by weight of Buza leaf, 200 parts by weight of Fagopyrum scabra, 150 parts by weight of chrysanthemum, 100 parts by weight of mulberry leaf, 100 parts by weight of Sophora japonica flower, 100 parts by weight of Lophatherum gracile, and 100 parts by weight of Licorice.

[0068] 2. Modeling: 1% ethylene glycol and 20 g / L hydroxyproline were used for modeling respectively. The model group continued to be modeled with 1% ethylene glycol; the combined tea group continued to be modeled, and medication began one week after modeling;

[0069] 3. Administration: one tea bag of combined tea is added to 500 mL of drinking water and boiled for 30 minutes, and the patient can drink water freely;

[0070] 4. Urine collection: Collect 24-hour urine once a week and freeze it for future use;

[0071] 5. Detection:

[0072] (1) Oxalic acid content detection

[0073] Steps:

[0074] Take an appropriate amount of urine sample for filtration, prepare the working solution according to the proportion of reagents described in the reagent manual according to the sample requirements, mix in a 1.5mL centrifuge tube, and take 100μL and add it to a 96-well plate for detection.

[0075] Determination steps:

[0076] 1) Preheat the spectrophotometer / ELISA reader for more than 30 minutes, adjust the wavelength to 510nm, and zero with distilled water;

[0077] 2) Dilute the 100 μmol / mL oxalic acid standard solution with distilled water to make 18, 15, 12, 6, 3, 1.5, 0.75, and 0.375 μmol / mL standard solutions for use.

[0078] 3) Operation table (operated in 1.5mL centrifuge tube / 96-well plate):

[0079] Mix the test reagents according to the kit instructions

[0080] Blank group: Add 10 μL of distilled water and 90 μL of the prepared assay reagent to each well and mix well.

[0081] Assay group: Add 10 μL of urine working solution and 90 μL of prepared assay reagent to each well and mix well.

[0082] Standard group: add 10 μL standard working solution and 90 μL prepared assay reagent to each well and mix well.

[0083] After standing at room temperature for 20 min, the absorbance value A at 510 nm was measured in a micro-glass cuvette / 96-well, and recorded as A 空白管 , A 测定管 , A 标准管 Calculate ΔA 测定 =A 空白管 -A 测定管 , ΔA 标准 =A 空白管 -A 标准管 For the same batch of test samples, 1-2 blank tubes need to be tested (standard tubes only need to be tested 1-2 times).

[0084] Calculation of oxalic acid content:

[0085] 1) Drawing of standard curve: With the concentration of each standard solution as the x-axis and the corresponding ΔA standard as the y-axis, draw the standard curve to obtain the standard equation y=kx+b, and substitute ΔA into the equation to obtain x (μmol / mL);

[0086] 2) Calculation of oxalic acid content: Oxalic acid content (mg / g mass) = x × V extraction × M × 10-3 ÷ W = 0.09x ÷ W

[0087] V extraction: volume of distilled water added, 1 mL; W: sample mass, g; M: molecular mass of oxalic acid, 90.04; 10 -3 :Unit conversion factor, 1μg=10 -3 mg.

[0088] Efficacy test results

[0089] As attached Figure 1 As shown, the Chinese herbal formula tea group of the present invention significantly reduced the urine oxalic acid of rats, compared with the model group *p < 0.05, **p < 0.01, ***p < 0.001, and the efficacy was significant; there was no statistical difference between the Chinese herbal formula tea group and the blank group at each time point, indicating that the formula tea would not affect the normal oxalic acid level.

[0090] (2) Citric acid content detection

[0091] Steps:

[0092] Citric acid extraction in liquid samples: Take 0.1mL of liquid and add 0.9mL of the reagent in the kit, mix thoroughly, centrifuge at 11000g, 4℃ for 10min, take the supernatant and place it on ice for testing. (If the measured value is low, the volume ratio of liquid sample and reagent can be appropriately adjusted, such as (0.2mL liquid sample + 0.8mL reagent) or (0.5mL liquid sample + 0.5mL reagent).)

[0093] Determination steps:

[0094] 1) Preheat the spectrophotometer for more than 30 minutes, adjust the wavelength to 545nm, and zero with distilled water;

[0095] 2) Preheat the reagent in a 30°C water bath for more than 30 minutes;

[0096] 3) Operation steps (operated in 1.5mL centrifuge tube / 96-well plate)

[0097] Mix the test reagents according to the kit instructions

[0098] Blank group: Add 10 μL of distilled water and 90 μL of the prepared assay reagent to each well and mix well.

[0099] Assay group: Add 10 μL of urine working solution and 90 μL of prepared assay reagent to each well and mix well.

[0100] Standard group: add 10 μL standard working solution and 90 μL prepared assay reagent to each well and mix well.

[0101] After fully mixing, let it stand at room temperature for 30 minutes, and measure the absorbance at 545nm, which is recorded as A 空白管 , A 测定管 , A 标准管 , calculate ΔA 测定 =A 测定管 -A 空白管 , ΔA 标准 =A 标准管 -A 空白管 The blank tube and standard tube only need to be done 1-2 times.

[0102] 4) Calculation of citric acid content: Calculated by liquid volume: Citric acid content (μmol / mL) = C standard solution × ΔA determination ÷ ΔA standard × F = 20 × ΔA determination ÷ ΔA standard

[0103] C standard solution: standard concentration; F: sample dilution factor, 10.

[0104] Notes: 1) Sample handling and other processes need to be carried out on ice; 2) Reagent 5 is a carcinogen. During the experiment, gloves must be worn to prevent Reagent 5 from splashing on the skin; 3) Citric acid reagent 1 cannot be used for protein content determination. If protein content needs to be determined, another tissue must be taken for determination; 4) If there are obvious small black particles after 30 minutes of reaction, it is normal and the sample needs to be diluted before testing; 5) If the sample absorbance value is greater than 0.5, it is recommended to dilute the sample with reagent 1 before testing.

[0105] Efficacy test results

[0106] As attached Figure 2As shown, the formula tea group made from the Chinese medicine composition of the present invention has a significant effect on increasing citric acid in rat urine, "*": compared with the blank group, *p<0.05, **p<0.01, ***p<0.001, ****p≤0.0001; "#": compared with the model group, #p<0.05, ##p<0.01, ###p<0.001, ####p≤0.0001.

[0107] As attached Figure 3 As shown, the formula tea group prepared by the Chinese medicine composition of the present invention has a better effect of increasing citric acid than potassium citrate, "*": compared with the blank group, *p<0.05, **p<0.01, ***p<0.001, ****p≤0.0001; "#": compared with potassium sodium hydrogen citrate, #p<0.05, ##p<0.01, ###p<0.001, ####p≤0.0001; "+": compared with the modeling group, +p<0.05, ++p<0.01, +++p<0.001, ++++p≤0.0001.

[0108] The above results show that the Chinese medicine composition of the present invention (Imperata root, Buza leaf, Fagopyrum scabra, chrysanthemum, mulberry leaf, Sophora japonica flower, Lophatherum gracile, and licorice) can significantly reduce the content of urinary oxalic acid in the body and increase the content of urinary citrate in the body, and can be used to prepare foods, medicines, and health products with the effects of reducing urinary oxalic acid and increasing urinary citrate.

Claims

1. A Chinese medicine composition having the effects of lowering urine oxalate and raising urine citric acid, characterized in that: It is composed of 8 main raw medicinal materials and secondary raw medicinal materials; The main raw medicinal materials include: Imperata root, Buza leaf, Fagopyrum sibiricum, Chrysanthemum, Mulberry leaf, Sophora japonica flower, Lophatherum gracile, and Licorice; the secondary raw medicinal materials include: Rhubarb, Catechu, Millettia reticulata, Lycium barbarum, Green fruit, Lophatherum venetum leaf, Smilax glabra, Kiwi fruit, Cinnamon bark, Kapok flower, Bletilla striata, Sanguisorba officinalis, Xanthium sibiricum, Alpinia officinalis, Safflower, and Thistle.

2. The Chinese medicine composition having the effects of lowering urine oxalate and raising urine citric acid as claimed in claim 1, characterized in that: The traditional Chinese medicine composition with the effects of reducing urine oxalic acid and increasing urine citric acid consists of the 8 main raw medicinal material components and 1-4 secondary raw medicinal material components.

3. The Chinese medicine composition having the effects of lowering urine oxalate and raising urine citric acid as claimed in claim 2, characterized in that: The traditional Chinese medicine composition with the effects of reducing urine oxalic acid and increasing urine citric acid is composed of the 8 main raw medicinal materials.

4. The Chinese medicine composition having the effects of lowering urine oxalate and raising urine citric acid according to claim 1, characterized in that: The main raw materials are as follows: 20-200 parts by weight of Imperata root, 20-200 parts by weight of Buza leaf, 60-600 parts by weight of Golden Buckwheat, 40-400 parts by weight of Chrysanthemum, 20-200 parts by weight of Mulberry leaf, 10-200 parts by weight of Sophora japonica flower, 20-200 parts by weight of Lophatherum gracile leaf, and 20-200 parts by weight of Licorice; The weight ratio of the secondary raw medicinal materials is as follows: 20-500 weight parts of rhubarb, 20-200 weight parts of catechu, 80-500 weight parts of Millettia spatholobi, 300-700 weight parts of wolfberry, 20-100 weight parts of green fruit, 20-200 weight parts of Apocynum venetum leaves, 20-400 weight parts of Smilax glabra, 100-800 weight parts of kiwi fruit, 10-100 weight parts of cinnamon bark, 20-100 weight parts of kapok flowers, 20-200 weight parts of white ampel, 70-200 weight parts of sanguisorba officinalis, 20-100 weight parts of Xanthium sibiricum, 400-800 weight parts of galangal, 100-500 weight parts of safflower, and 100-500 weight parts of thistle.

5. The Chinese medicine composition having the effects of lowering urine oxalate and raising urine citric acid as claimed in claim 4, characterized in that: The main raw materials are as follows: 20-100 parts by weight of Imperata root, 60-100 parts by weight of Buza leaf, 100-400 parts by weight of Fagopyrum truncatum, 100-200 parts by weight of Chrysanthemum, 20-100 parts by weight of Mulberry leaf, 60-120 parts by weight of Sophora japonica flower, 20-100 parts by weight of Lophatherum gracile, and 20-100 parts by weight of Licorice. The weight ratio of the secondary raw medicinal materials is: 100-300 parts by weight of rhubarb, 100-200 parts by weight of catechu, 100-300 parts by weight of Millettia spatholobi, 400-600 parts by weight of wolfberry, 40-80 parts by weight of green fruit, 40-100 parts by weight of Apocynum venetum leaf, 40-300 parts by weight of Smilax glabra, 200-600 parts by weight of kiwi fruit, 20-80 parts by weight of cinnamon bark, 40-80 parts by weight of kapok flower, 50-100 parts by weight of white ampel, 90-150 parts by weight of Sanguisorba officinalis, 40-80 parts by weight of Xanthium sibiricum, 650-750 parts by weight of galangal, 200-400 parts by weight of safflower, and 200-400 parts by weight of thistle.

6. The Chinese medicine composition having the effects of lowering urine oxalate and raising urine citric acid as claimed in claim 5, characterized in that: The weight ratio of the main raw medicinal materials is as follows: 20-50 parts by weight of Imperata root, 80-100 parts by weight of Buza leaf, 200-400 parts by weight of Golden Buckwheat, 150-200 parts by weight of Chrysanthemum, 20-60 parts by weight of Mulberry leaf, 80-100 parts by weight of Sophora japonica flower, 20-50 parts by weight of Lophatherum gracile leaf, and 20-50 parts by weight of Licorice root; The weight ratio of secondary raw medicinal materials: 180-240 weight parts of rhubarb, 120-180 weight parts of catechu, 150-250 weight parts of Millettia spatholobi, 450-550 weight parts of wolfberry, 50-70 weight parts of green fruit, 60-80 weight parts of dogbane leaves, 50-200 weight parts of smilax glabra, 300-500 weight parts of kiwi fruit, 30-60 weight parts of cinnamon bark, 50-70 weight parts of kapok, 60-80 weight parts of white ampel, 100-130 weight parts of sanguisorba officinalis, 50-70 weight parts of Xanthium sibiricum, 680-720 weight parts of galangal, 280-340 weight parts of safflower, and 280-340 weight parts of thistle.

7. The Chinese medicine composition having the effects of lowering urine oxalate and raising urine citric acid as claimed in claim 3, characterized in that: The Chinese medicine composition having the effects of lowering urine oxalate and raising urine citric acid is composed of the following medicinal materials in weight ratio: 100 parts by weight of Imperata rhizome, 50 parts by weight of Buza leaves, 200 parts by weight of Fagopyrum truncatum, 150 parts by weight of chrysanthemum, 100 parts by weight of mulberry leaves, 100 parts by weight of Sophora japonica flower, 100 parts by weight of Lophatherum gracile, and 100 parts by weight of Licorice.

8. Use of the Chinese medicine composition according to any one of claims 1 to 3 in the preparation of a product having the effects of lowering urine oxalate and raising urine citric acid, characterized in that: The products include food, medicine and health products.

9. The use according to claim 8, characterized in that The product is a medicine or a health product, and the medicine also includes a pharmaceutically acceptable carrier and / or excipient; the dosage form of the medicine or health product is tablets, powders, granules, capsules, emulsions or suspensions.

10. The use according to claim 8, characterized in that The product is a food, and the food is at least one of tea bags, health tea, solid food, and liquid beverage.

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