Electrostatic spinning film fixing device, stacking device, winding device and preparation system

Abstract

The invention relates to an electrostatic spinning film fixing device, a stacking device, a winding device and a preparation system, which are suitable for ultra-thin electrostatic spinning films. The electrostatic spinning film fixing device comprises a first fixing piece, a second fixing piece and a third fixing piece, the first fixing piece is arranged on the first side of a film, and the first fixing piece is provided with at least two subareas; and the second fixing piece is configured to be arranged on the second side of the thin film, and the second fixing piece is tightly clamped with the boundary part of each subarea of the at least two subareas, so that the thin film forms a thin film unit in each subarea. According to the electrostatic spinning film fixing device, the electrostatic spinning film can be directly fixed on a preparation device of the electrostatic spinning film, the electrostatic spinning film is fixed in a clamping mode, operation is convenient, and the electrostatic spinning film cannot be damaged.

Description

Technical Field

[0001] The present invention relates to the field of film material production, and in particular to an electrostatic spinning film fixing device, a stacking device, a winding device and a preparation system. Background Art

[0002] Electrospinning is a method for manufacturing non-woven fiber materials: the molten or dissolved material forms a jet under the action of a high-voltage electric field, which stretches and solidifies in the air to form fiber filaments of micrometer to nanometer scale, which are then collected on a collector and accumulated into a macroscopic membrane-like porous fiber material.

[0003] A variety of synthetic and natural materials are suitable for electrospinning technology. By adjusting parameters such as material concentration, material flow rate, voltage, collector shape and structure (and rotation), collection time, etc., the fiber diameter, filament spacing, fiber morphology and other structural properties can be adjusted to control their macroscopic physical properties. The chemical properties can also be adjusted by adding composite materials or post-processing processes.

[0004] The micro-nano fiber structure, high surface area ratio, various composite materials and structures of electrospinning materials make this material have many applications and a large market in the fields of medical treatment, energy storage and conversion, green agriculture, environmental protection, smart wearables, electromagnetic shielding, chemical catalysis, food packaging, etc.

[0005] Making electrospun materials thin is a requirement for many applications, such as improving the transmittance of energy storage materials, improving cell permeability for transplanted tissues, improving the breathability of skin dressings, improving the functionality, convenience and comfort of multi-layer wearable devices or special functional clothing materials (such as high temperature resistant protective clothing), etc. Therefore, making electrospun materials thin is one of the important development directions of this material. However, electrospun materials are generally very soft, and reducing the thickness will make them difficult to prepare, characterize or operate, such as physical operations such as picking, standing, flipping, multi-layer stacking, winding, biochemical operations such as surface chemical modification, sterilization post-treatment, and biological application operations such as planting cells on (in) electrospun materials. When the electrospun material is too thin, these operations are difficult to achieve. Moreover, some materials cannot be "self-standing" (i.e., they cannot maintain a thin film morphology by themselves) because they are too thin. They will shrink or curl rapidly in the air or liquid without external clamping, and lose their flat film morphology. These difficulties result in the thickness of the electrospinning membrane materials commonly used currently being between hundreds of micrometers and several millimeters, which greatly hinders the application of this material in the above-mentioned fields. Summary of the invention

[0006] Some embodiments of the present invention provide an electrospinning film fixing device, a stacking device, a winding device and a preparation system, which are suitable for extremely thin electrospinning films.

[0007] In one aspect of the present invention, there is provided an electrospinning film fixing device, comprising:

[0008] A first fixing member configured to be disposed on a first side of the film, the first fixing member having at least two partitions; and

[0009] The second fixing member is configured to be disposed on the second side of the film, and the second fixing member is clamped with the boundary portion of each of the at least two partitions, so that the film forms a film unit in each partition.

[0010] In some embodiments, one of the first fixing member and the second fixing member is provided with a slot, and the other of the first fixing member and the second fixing member is provided with a buckle, and the slot and the buckle cooperate to achieve the clamping of the first fixing member and the second fixing member.

[0011] In some embodiments, the second fixing member includes an annular member, and the annular member is circumferentially and closedly disposed along the boundary of the partition.

[0012] In some embodiments, the first fixing member includes a first annular member, which is configured to form one of the at least two partitions, and the second fixing member includes a second annular member, which is configured to be disposed in the first annular member and clamped and fixed to the first annular member.

[0013] In some embodiments, the first annular member is provided with a first slot, and the second annular member is provided with a first buckle, and the first buckle is configured to be disposed in the first slot.

[0014] In some embodiments, the first fixing member includes a first plate and at least two first ring-shaped members, and the at least two first ring-shaped members are detachably disposed on the first plate to form the at least two partitions.

[0015] In some embodiments, the second fixing member includes at least two second annular members, and the at least two second annular members are configured to be respectively disposed in the at least two first annular members in a one-to-one correspondence, and the second annular members are clamped with the first annular members.

[0016] In some embodiments, the second fixing member includes a second plate, the at least two second annular members are detachably disposed on the second plate, the second plate is configured to carry the at least two second annular members respectively and one-to-one correspond to the at least two first annular members disposed on the first plate, and the first plate and the second plate are configured to be disassembled after the first annular member and the second annular member are clamped.

[0017] In some embodiments, the first annular member is provided with a first slot, the first plate is provided with a first groove for accommodating the first annular member, the side wall of the first groove is provided with a second buckle, and the second buckle is configured to be provided in the first slot.

[0018] In some embodiments, the second annular member is provided with a first protrusion, the second plate is provided with a second groove for accommodating the second annular member, a through hole is provided in the second groove, and the first protrusion is configured to be inserted into the through hole.

[0019] In some embodiments, the electrospinning film fixing device also includes a third plate, which is provided with a second protrusion. The third plate is configured to be arranged on the side of the second plate opposite to the second groove, and the second protrusion is configured to be inserted into the through hole and push the first protrusion.

[0020] In some embodiments, the second fixing member includes two strip members, and the two strip members are respectively arranged on opposite sides of the boundary of the partition in a one-to-one correspondence.

[0021] In some embodiments, the first fixing member includes a strip partition, and second slots are respectively provided on both sides of the strip partition. The two strip members are respectively provided with third buckles, and the third buckles are configured to be arranged in the second slots so that the strip members are clamped and fixed to the first fixing member.

[0022] In one aspect of the present invention, there is provided an electrospinning film stacking device, comprising:

[0023] A first component, the top of which is provided with a receiving groove;

[0024] A third annular member, the outer diameter of which is smaller than the inner circumferential dimension of the receiving groove; the third annular member is configured to be disposed in the receiving groove;

[0025] The above-mentioned electrospinning film fixture; the film unit formed by the fixture is configured to be placed on top of the first component, and the circumferential dimension of the film unit is larger than the circumferential dimension of the first component;

[0026] A second component, whose circumferential dimension is smaller than the inner circumferential dimension of the containing groove; the second component is configured to abut against the membrane unit from the top of the membrane unit;

[0027] a fifth annular member, whose inner diameter is larger than the circumferential dimensions of the second member and the first member; the fifth annular member is configured to be sleeved on the second member and to press the film unit so that the outer edge of the film unit is circumferentially wrapped around the outer circumference of the first member; and

[0028] The fourth ring-shaped member has an outer diameter smaller than the inner diameter of the third ring-shaped member; the fourth ring-shaped member is configured to be arranged on the inner side of the third ring-shaped member and clamped with the third ring-shaped member after the plurality of the film units are stacked in sequence and the outer edges of the plurality of the film units are circumferentially wrapped around the outer circumference of the first member in sequence; the third ring-shaped member is configured to cooperate with the fourth ring-shaped member to fix the stacked plurality of film units.

[0029] In one aspect of the present invention, there is provided an electrospinning film winding device, comprising:

[0030] Strip board;

[0031] The above-mentioned electrospinning film fixing device; the fixing device is configured to form a strip-shaped film unit, and the film unit is configured to be attached to the top surface of the strip plate; and

[0032] The film-breaking member is configured to disconnect the film unit from the fixing device along the length direction of the film unit, and the film unit is wound into a roll.

[0033] In some embodiments, the electrospinning film winding device further comprises a reel, wherein the reel is configured to be disposed at an end of the film unit so that the film unit is wound around the reel.

[0034] In some embodiments, the film breaking component comprises:

[0035] A first fixed block;

[0036] a second fixing block; and

[0037] Two shearing pieces, each of which includes a first blade and a second blade, and the two first blades are fixedly arranged between the first fixing block and the second fixing block at intervals; the two shearing pieces are configured to simultaneously cut off the connection between the film unit and the fixing device along the length direction of the film unit.

[0038] In some embodiments, the film breaking component comprises:

[0039] a first main body member;

[0040] a first side member;

[0041] a second side member; and

[0042] Two blades, one of which is fixed to the first side of the first main body through the first side piece, and the other is fixed to the second side of the first main body through the second side piece; the two blades are configured to simultaneously cut off the connection between the film unit and the fixing device along the length direction of the film unit.

[0043] In one aspect of the present invention, there is provided an electrospinning film preparation system, comprising:

[0044] power supply;

[0045] A wire collector is configured to rotate, the wire collector comprising:

[0046] at least two rods connected to the negative pole of the power source or to ground;

[0047] A first connecting member connected to the first ends of the at least two rods; and

[0048] A second connecting member connected to the second ends of the at least two rods;

[0049] an injection member connected to the positive electrode of the power supply; the injection member is configured to spray a solution toward the collector to form an electrospinning film on the at least two rods; and

[0050] The above-mentioned electrospun film fixing device is configured to fix the electrospun film.

[0051] In some embodiments, the electrospinning film preparation system further includes a guide rail, the injection member is movably disposed on the guide rail, and an extension direction of the guide rail is parallel to an extension direction of the at least two rods.

[0052] In some embodiments, the electrospinning film preparation system also includes a first test component, which includes a first frame and two clamping members; the first frame includes a first frame bar and a second frame bar that are relatively arranged, and a third frame bar and a fourth frame bar that are relatively arranged, and the first frame bar and the second frame bar are respectively provided with a first breakable portion, and the two clamping members are respectively and one by one arranged on the third frame bar and the fourth frame bar to clamp the electrospinning film, and the first breakable portion is configured to break so as to perform a tensile test on the electrospinning film.

[0053] In some embodiments, the electrospinning film preparation system also includes a second test component, which includes two second frames, and one of the two second frames has a second breakable portion on both sides thereof. The two second frames are configured to clamp the electrospun film and be immersed in nitrogen liquid. The second breakable portions of the two second frames are configured to break so as to perform a brittle fracture test on the electrospun film.

[0054] In some embodiments, the electrospinning film preparation system also includes a third test component, which includes a plate and a conductive adhesive, wherein the plate is provided with a boss, the conductive adhesive is configured to press a recess through the boss, and the conductive adhesive is configured to carry the electrospinning film to perform an electron microscope scanning test on the electrospinning film.

[0055] In some embodiments, the electrospinning film preparation system also includes a fourth test component, which includes a sixth ring-shaped member and a seventh ring-shaped member, the outer diameter of the sixth ring-shaped member is smaller than the inner diameter of the seventh ring-shaped member, the sixth ring-shaped member is arranged on the first side of the electrospinning film, and the seventh ring-shaped member is configured to be arranged on the second side of the electrospinning film, the sixth ring-shaped member is configured to be arranged in the seventh ring-shaped member and protrude from the seventh ring-shaped member, the sixth ring-shaped member is configured to clamp the electrospinning film with the seventh ring-shaped member, and the portion of the sixth ring-shaped member protruding from the seventh ring-shaped member is covered with the electrospinning film, so as to perform a hydrophilicity and hydrophobicity test on the electrospinning film.

[0056] Based on the above technical solution, the present invention has at least the following beneficial effects:

[0057] In some embodiments, the first fixing member of the electrospinning film fixing device is arranged on the first side of the film, and the first fixing member has at least two partitions; the second fixing member is arranged on the second side of the film, and the second fixing member is clamped with the boundary portion of each of the at least two partitions so that the film forms a film unit in each partition, and the electrospinning film can be directly fixed on the electrospinning film preparation device, and the electrospinning film is fixed by clamping, which is easy to operate and will not cause damage to the electrospinning film. BRIEF DESCRIPTION OF THE DRAWINGS

[0058] The drawings described herein are used to provide a further understanding of the present invention and constitute a part of this application. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention. In the drawings:

[0059] Figure 1 A schematic diagram of an electrospinning film preparation device according to some embodiments of the present invention;

[0060] Figure 2A schematic diagram of a first embodiment of a wire collector provided according to the present invention;

[0061] Figure 3 A schematic diagram of a second embodiment of a wire collector provided according to the present invention;

[0062] Figure 4 A schematic diagram of the matching structure of a first annular member and a second annular member provided according to some embodiments of the present invention;

[0063] Figure 5 A schematic diagram of a second annular member provided according to some embodiments of the present invention;

[0064] Figure 6 A schematic diagram of some components of a first embodiment of an electrospinning film fixing device provided according to the present invention;

[0065] Figure 7 A schematic diagram of the operation process of the first embodiment of the electrospinning film fixing device provided by the present invention;

[0066] Figure 8 A schematic diagram of a second embodiment of an electrospinning film fixing device provided according to the present invention;

[0067] Fig. 9 A side view schematic diagram of a second embodiment of the electrospinning film fixing device provided according to the present invention;

[0068] Fig.10 A schematic diagram of the cooperation between the second card slot and the third card buckle of the second embodiment of the electrospinning film fixing device provided by the present invention;

[0069] Fig.11 This is a schematic diagram showing that the third buckle of the second embodiment of the electrospinning film fixing device provided by the present invention is located in the second buckle groove;

[0070] Fig.12 A schematic diagram of the operation process of the second embodiment of the electrospinning film fixing device provided by the present invention;

[0071] Fig.13 A schematic diagram of some components of an electrospinning film stacking device provided according to some embodiments of the present invention;

[0072] Fig.14 A schematic diagram of the operation process of an electrospinning film stacking device provided according to some embodiments of the present invention;

[0073] Fig.15 A schematic diagram of the operation process of the first embodiment of the electrospinning film stacking device provided by the present invention;

[0074] Fig.16A schematic diagram of a first embodiment of a film breaking member provided according to the present invention;

[0075] Fig.17 A schematic diagram of a second embodiment of a film breaking member provided according to the present invention;

[0076] Fig.18 A schematic diagram of the operation process of the second embodiment of the electrospinning film winding device provided by the present invention;

[0077] Fig.19 A schematic diagram of an electrospinning film roll fixing device provided according to some embodiments of the present invention;

[0078] Fig. 20 A schematic diagram of a first test assembly provided according to some embodiments of the present invention;

[0079] Fig.21 A schematic diagram of a second test assembly provided according to some embodiments of the present invention;

[0080] Fig. 22 A schematic diagram of a third test assembly provided according to some embodiments of the present invention;

[0081] Fig.23 A schematic diagram of a fourth test assembly provided according to some embodiments of the present invention;

[0082] Fig.24 A schematic cross-sectional view of a scaffold material of stacked multi-layer electrospun films provided according to some embodiments of the present invention;

[0083] Fig.25 This is a schematic diagram of a statically electrospun film wound up using a reel according to some embodiments of the present invention.

[0084] The reference numerals in the accompanying drawings are described as follows:

[0085] 100-electrospinning film fixing device;

[0086] 110-first fixing member; 111-partition; 112-first annular member; 113-first slot; 114-first plate; 1141-positioning hole; 115-first groove; 1151-second buckle; 116-strip partition; 117-second slot; 1171-first clamping portion; 1172-second clamping portion; 118-convex portion;

[0087] 120-second fixing member; 121-second annular member; 122-first buckle; 123-second plate; 124-positioning column; 125-first protrusion; 126-second groove; 127-through hole; 128-strip member; 129-third buckle; 1291-third clamping portion; 1292-fourth clamping portion;

[0088] 130 - third plate; 131 - second protrusion;

[0089] 200 - electrospinning film stacking device; 210 - first component; 220 - second component; 230 - third annular member; 240 - fourth annular member; 250 - fifth annular member;

[0090] 300-Electrospinning film winding device;

[0091] 310-strip plate; 311-recess;

[0092] 320-film cutting member; 321-first fixing block; 322-second fixing block; 323-shearing member; 3231-first blade member; 3232-second blade member; 324-first main body member; 325-first side member; 326-second side member; 327-blade;

[0093] 330-Scroll;

[0094] 400-Electrospinning film preparation device;

[0095] 410-power supply;

[0096] 420-wire collector; 421-rod; 422-first connecting member; 423-second connecting member;

[0097] 430-injection member; 440-guide rail; 450-carrying member; 460-motor;

[0098] 500-first test assembly; 510-first frame; 511-first frame bar; 512-second frame bar; 513-third frame bar; 514-fourth frame bar; 520-clamping member; 530-first fragile portion;

[0099] 600 - second test assembly; 610 - second frame; 620 - second breakable portion;

[0100] 700-third test assembly; 710-plate; 711-boss; 720-conductive adhesive; 730-pressing piece;

[0101] 800 - fourth test assembly; 810 - sixth annular member; 820 - seventh annular member;

[0102] 900 - electrostatic spinning film roll fixing device; 910 - third fixing member; 920 - fourth fixing member.

[0103] It should be understood that the size of each part shown in the accompanying drawings is not drawn according to the actual proportional relationship. In addition, the same or similar reference numerals represent the same or similar components. DETAILED DESCRIPTION

[0104] Various exemplary embodiments of the present invention will now be described in detail with reference to the accompanying drawings. The description of the exemplary embodiments is merely illustrative and is in no way intended to limit the present invention and its application or use. The present invention can be implemented in many different forms and is not limited to the embodiments described herein. These embodiments are provided to make the present invention thorough and complete and to fully express the scope of the present invention to those skilled in the art. It should be noted that unless otherwise specifically stated, the relative arrangement of the parts and steps, the composition of the materials, the numerical expressions and the numerical values ​​set forth in these embodiments should be interpreted as being merely exemplary, rather than as limitations.

[0105] The words "first", "second" and similar words used in the present invention do not indicate any order, quantity or importance, but are only used to distinguish different parts. The words "include" or "comprise" and similar words mean that the elements before the word include the elements listed after the word, and do not exclude the possibility of including other elements. "Up", "down", "left", "right" and the like are only used to indicate relative positional relationships. When the absolute position of the described object changes, the relative positional relationship may also change accordingly.

[0106] In the present invention, when a specific device is described as being located between a first device and a second device, there may or may not be an intermediate device between the specific device and the first device or the second device. When a specific device is described as being connected to other devices, the specific device may be directly connected to the other devices without an intermediate device, or may not be directly connected to the other devices but have an intermediate device.

[0107] All terms (including technical terms or scientific terms) used in the present invention have the same meanings as those understood by ordinary technicians in the field to which the present invention belongs, unless otherwise specifically defined. It should also be understood that terms defined in general dictionaries should be interpreted as having meanings consistent with their meanings in the context of the relevant technology, and should not be interpreted in an idealized or extremely formal sense, unless explicitly defined herein.

[0108] Technologies, methods, and equipment known to ordinary technicians in the relevant art may not be discussed in detail, but where appropriate, the technologies, methods, and equipment should be considered part of the specification.

[0109] Electrospinning is a method for manufacturing non-woven fiber materials: the molten or dissolved material forms a jet under the action of a high-voltage electric field, which stretches and solidifies in the air to form fiber filaments of micrometer to nanometer scale, which are then collected on a collector and accumulated into a macroscopic membrane-like porous fiber material.

[0110] Electrospinning materials have a wide range of uses in medical treatment, energy storage and conversion, green agriculture, environmental protection, smart wearables, electromagnetic shielding, chemical catalysis, food packaging and other fields. Making electrospinning materials thin is the key to many applications and is also the overall development trend of this material.

[0111] In terms of preparation, the first step in making electrospun films requires that the collector can collect the spinning in mid-air, that is, the film to be collected cannot be attached to the surface of the collector (the static metal plate or rotating drum used in some related technologies will not be applicable), because the electrospun film cannot withstand the external forces such as peeling, pulling, and rolling when it is removed from the non-suspended collector surface, and will be overly deformed or damaged. The static parallel collector method that currently meets this requirement has low production efficiency, a small area of ​​prepared film, and complex operation, and low potential for mass production.

[0112] To manufacture electrospun films, it is necessary to fix the films on the fixture to maintain the macroscopic shape of the films and the microscopic morphology of the fibers. In some related technologies, the methods for fixing electrospun films are all implemented around the structure of inserts. For example, after removing the original film of the insert, the electrospun film is glued to the end face of the insert body with PDMS (polydimethylsiloxane). This method is cumbersome to operate, time-consuming (it takes several hours to cure PDMS), requires heating (generally above 65°C, so it is not suitable for some temperature-sensitive materials), and is not easy to reuse. Another example: use neutral silica gel to glue the electrospun film to the insert from which the original film has been removed. In order to implant the film into the body, medical sutures are also glued into a circle and spun between two layers of electrospun films of the same thickness to fix the electrospun film. However, because the mechanical strength of medical sutures is very low, this method cannot maintain the morphology and flatness of large-area electrospun film materials, and the operation is complicated, it cannot be reused, and the manual error is large. In addition to these devices based on plug-in modification, some related technologies also have plug-in products specifically for clamping thin film materials. First of all, these products have different design defects, which will cause micro and macro damage to the spinning support during the fixing or clamping production process.

[0113] In terms of characterization, electrospun materials usually need to be observed by scanning electron microscopy, mechanical properties by tensile tests, thickness by embedding and cross-section, hydrophilicity by contact angle meter, etc. Because the electrospun film is too thin, these characterization processes will cause operational difficulties.

[0114] More importantly, the methods and devices in the related art can only realize simple operations such as picking up and standing still, and cannot realize complex operations such as flipping, multi-layer stacking, and winding, which are necessary in the above-mentioned multiple application fields. For example, in order to cooperate with the use in the cell culture well plate, the plug-in can only be inserted in one direction and cannot be flipped. Moreover, making electrospinning thin is usually a requirement for one of the processes to achieve a certain function of the product, and in order to finally obtain a strong and usable material, it is necessary to stack or wind the 2D electrospun film into a 3D material with a certain thickness. Therefore, stacking and winding electrospun films is a key technical problem. However, the vast majority of stacking and winding electrospun materials in the related art are operated on self-supporting, thick electrospun membranes of more than 100 microns. There is no good method for stacking and winding non-self-supporting electrospun films.

[0115] In summary, there is no comprehensive solution or dedicated device in the related art for electrospun films that can be reused multiple times, is easy to assemble and disassemble, does not damage the fiber structure, and covers preparation, characterization, and operation.

[0116] Based on this, some embodiments of the present invention provide an electrospinning film preparation system including a preparation device and process suitable for ultra-thin electrospinning membrane materials (electrospinning films), a characterization device and process, and a device and process including operations such as stacking and winding, to alleviate the above-mentioned problems.

[0117] In some embodiments, the electrospinning film preparation system includes an electrospinning film preparation device 400 .

[0118] refer to Figure 1 Some embodiments provide an electrospinning film preparation device 400 , which includes a power source 410 , a collector 420 and a jet element 430 .

[0119] The wire collector 420 includes at least two rods 421, a first connecting member 422 and a second connecting member 423. The rod 421 is a metal rod.

[0120] At least two rods 421 are connected to the negative pole of the power source 410 or to the ground.

[0121] The first connecting member 422 is connected to the first ends of at least two rods 421 .

[0122] The second connecting member 423 is connected to the second ends of at least two rods 421 .

[0123] The injection member 430 is connected to the positive electrode of the power source 410 .

[0124] The collector 420 is configured to rotate, and the spraying member 430 is configured to spray the solution toward the collector 420 during the rotation of the collector 420 , so as to form an electrospinning film on at least two rods 421 .

[0125] The injection member 430 includes a syringe pump, which includes a syringe, a needle, and a push member, which pushes the solution in the syringe to eject the solution through the needle.

[0126] A surface for collecting the electrospun film is formed between the two rods 421 .

[0127] In some embodiments, the electrospinning film preparation device 400 further includes a motor 460 , which is drivingly connected to the wire collector 420 , and the motor 460 drives the wire collector 420 to rotate.

[0128] In some embodiments, the electrospinning film preparation device 400 further includes a guide rail 440 , and the injection member 430 is movably disposed on the guide rail 440 . The extension direction of the guide rail 440 is parallel to the extension direction of the at least two rods 421 .

[0129] The spraying member 430 reciprocates along the guide rail 440 during the process of spraying the solution, so as to form an electrospinning film on a plane formed by at least two rods 421 .

[0130] refer to Figure 2 In the first embodiment, the collector 420 includes three or more rods 421. The plane formed between two adjacent rods 421 is used to form an electrospinning film. The first end of each rod 421 is connected to the first connecting member 422, and the second end of each rod 421 is connected to the second connecting member 423. The first connecting member 422 and the second connecting member 423 are supported by a bracket. The motor 460 is driven and connected to the second connecting member 423 so that the second connecting member 423, each rod 421 and the first connecting member 422 rotate synchronously.

[0131] refer to Figure 3 In the second embodiment, the collector 420 includes two rods 421. The plane formed between the two rods 421 is used to form two layers of electrospinning film. Since the distance between the two layers of electrospinning film is small, it can be used as one layer of electrospinning film. The first ends of the two rods 421 are connected to the first connecting member 422, and the second ends of the two rods 421 are connected to the second connecting member 423. The first connecting member 422 and the second connecting member 423 are supported by a bracket. The motor 460 is driven and connected to the second connecting member 423 so that the second connecting member 423, the two rods 421 and the first connecting member 422 rotate synchronously.

[0132] In some embodiments, the electrospinning film preparation system includes an electrospinning film fixture 100 .

[0133] refer to Figures 4 to 12 In some embodiments, the electrospinning film fixing device 100 includes a first fixing member 110 and a second fixing member 120 .

[0134] refer to Figure 7 The first fixing member 110 is configured to be disposed on the first side of the film, and the first fixing member 110 has at least two partitions 111 .

[0135] The second fixing member 120 is configured to be disposed on the second side of the film. The second fixing member 120 is clamped with the boundary portion of each of the at least two partitions 111 so that the film forms a film unit in each partition 111 .

[0136] In some embodiments, one of the first fixing member 110 and the second fixing member 120 is provided with a slot, and the other of the first fixing member 110 and the second fixing member 120 is provided with a buckle, and the slot and the buckle cooperate to achieve the clamping of the first fixing member 110 and the second fixing member 120.

[0137] refer to Figures 4 to 7 , the electrospinning film fixture 100 includes a circular partition 111 .

[0138] In some embodiments, the second fixing member 120 includes an annular member, which is circumferentially closed and disposed along the boundary of the partition 111 .

[0139] refer to Figure 4 In some embodiments, the first fixing member 110 includes a first annular member 112, which is configured to form one of at least two partitions 111, and the second fixing member 120 includes a second annular member 121, which is configured to be disposed in the first annular member 112 and clamped and fixed to the first annular member 112.

[0140] refer to Figure 4 and Figure 5 In some embodiments, the first annular member 112 is provided with a first slot 113 , and the second annular member 121 is provided with a first buckle 122 , and the first buckle 122 is configured to be disposed in the first slot 113 .

[0141] refer to Figure 6 and Figure 7 In some embodiments, the first fixing member 110 includes a first plate 114 and at least two first annular members 112 , and the at least two first annular members 112 are detachably disposed on the first plate 114 to form at least two partitions 111 .

[0142] refer to Figure 4 and Figure 7In some embodiments, the second fixing member 120 includes at least two second annular members 121 , and the at least two second annular members 121 are respectively and one by one arranged in at least two first annular members 112 , and the second annular members 121 are clamped with the first annular members 112 .

[0143] refer to Figure 4 and Figure 7 In some embodiments, the second fixing member 120 includes a second plate 123, and at least two second annular members 121 are detachably provided on the second plate 123. The second plate 123 is configured to carry at least two second annular members 121, which are respectively and one-to-one arranged in at least two first annular members 112 on the first plate 114, and the first plate 114 and the second plate 123 are configured to be disassembled after the first annular member 112 and the second annular member 121 are clamped.

[0144] refer to Figure 4 and Figure 6 In some embodiments, the first annular member 112 is provided with a first card slot 113, the first plate 114 is provided with a first groove 115 for accommodating the first annular member 112, the side wall of the first groove 115 is provided with a second card buckle 1151, and the second card buckle 1151 is configured to be arranged in the first card slot 113.

[0145] refer to Figure 5 and Figure 6 In some embodiments, the second annular member 121 is provided with a first protrusion 125 , the second plate 123 is provided with a second groove 126 for accommodating the second annular member 121 , a through hole 127 is provided in the second groove 126 , and the first protrusion 125 is configured to be inserted into the through hole 127 .

[0146] refer to Figure 6 and Figure 7 In some embodiments, the electrospinning film fixing device 100 also includes a third plate 130, and the third plate 130 is provided with a second protrusion 131. The third plate 130 is configured to be arranged on the side of the second plate 123 opposite to the second groove 126, and the second protrusion 131 is configured to be inserted into the through hole 127 and push the first protrusion 125.

[0147] refer to Figure 7 In the first embodiment, the operation process of the electrospinning film fixing device 100 is as follows:

[0148] A first annular member 112 is respectively mounted in each of the first grooves 115 on the first plate 114 of the first fixing member 110 ; a second annular member 121 is respectively mounted in each of the second grooves 126 on the second plate 123 of the second fixing member 120 .

[0149] The electrospun film on one side of the collector 420 is placed on the top of the first fixing member 110 .

[0150] The collector 420 and the first fixing member 110 are turned upside down together, with the first fixing member 110 on the upper side and the electrospinning film on the lower side.

[0151] The second fixture 120 is assembled with the first fixture 110 from below the electrospun film.

[0152] The entire assembly is turned upside down, with the second fixing member 120 being above the electrospun membrane and the first fixing member 110 being below the electrospun membrane.

[0153] Assemble the third plate 130 with the second plate 127 of the second fixing member 120, press the third plate 130, insert the second protrusion 131 on the third plate 130 into the through hole 127 on the second plate 123, and push the first protrusion 125, so that the second annular member 121 and the first annular member 112 clamp and fix the electrospinning film.

[0154] The third plate 130 , the first plate 114 and the second plate 123 are disassembled, and the electrospun film on the collector 420 is cut to obtain a plurality of electrospun film units fixed by the first annular member 112 and the second annular member 121 .

[0155] refer to Figures 8 to 12 The electrospinning film fixture 100 includes a strip-shaped partition 111 .

[0156] refer to Figure 8 In some embodiments, the second fixing member 120 includes two strip members 128 , and the two strip members 128 are respectively disposed on opposite sides of the boundary of the partition 111 in a one-to-one correspondence.

[0157] refer to Figure 8 In some embodiments, the first fixing member 110 includes a strip partition 116, and second slots 117 are respectively provided on both sides of the strip partition 116. The two strip members 128 are respectively provided with third buckles 129, and the third buckles 129 are configured to be arranged in the second slots 117 so that the strip members 128 are clamped and fixed to the first fixing member 110.

[0158] refer to Figure 8 and Fig. 9 In some embodiments, two strip-shaped partitions 116 are provided on the first fixing member 110 , and a second slot 117 is provided on both sides of each strip-shaped partition 116 , and a strip-shaped member 128 is provided in each second slot 117 .

[0159] refer to Fig. 9In some embodiments, a convex portion 118 protruding upward is provided between the side of the strip partition 116 and the adjacent second clamping groove 117. After the electrospun film covers the strip partition 116 and is clamped by the second clamping grooves 117 and the strip member 128 on both sides of the strip partition 116, the electrospun film is supported by the convex portion 118 and protrudes in a direction away from the strip partition 116, so that operations such as cell culture can be performed on the electrospun film.

[0160] refer to Fig.10 and Fig.11 In some embodiments, a first clamping portion 1171 and a second clamping portion 1172 are provided at the upper portion of the second slot 117. The first clamping portion 1171 and the second clamping portion 1172 are arranged relative to each other. The first clamping portion 1171 and the second clamping portion 1172 both protrude into the second slot 117, and the surface of the second clamping portion 1172 gradually tilts into the second slot 117 along the direction from top to bottom.

[0161] refer to Fig.10 and Fig.11 In some embodiments, the third clip 129 is respectively provided with a third clip portion 1291 and a fourth clip portion 1292, the third clip portion 1291 and the fourth clip portion 1292 are arranged opposite to each other, the third clip portion 1291 is provided with an arc surface cooperating with the first clip portion 1171, and the fourth clip portion 1292 is provided with an inclined surface cooperating with the second clip portion 1172.

[0162] The process of installing the third clip 129 into the second clip slot 117 is as follows: first, the third clip part 1291 is abutted against the first clip part 1171, and the inclined surface of the fourth clip part 1292 and the inclined surface of the second clip part 1172 gradually fit together to squeeze the third clip 129, so that the fourth clip part 1292 slides to the bottom of the second clip part 1172, and the third clip part 1291 slides to the bottom of the first clip part 1171, and the third clip 129 is installed in the second clip slot 117.

[0163] refer to Fig.12 In the second embodiment, the operation process of the electrospinning film fixing device 100 is as follows:

[0164] The electrospun film on one side of the collector 420 is placed on the first fixing member 110 having two strip-shaped partitions 116;

[0165] The two strip members 128 are respectively disposed in the second slots 117 outside the two strip partitions 116;

[0166] Cutting the electrospun film on the collector 420;

[0167] The other two strip-shaped members 128 are respectively disposed in the second slots 117 inside the two strip-shaped partitions 116 to obtain two strip-shaped electrospinning film units.

[0168] In some embodiments, the electrospinning film preparation system includes an electrospinning film stacking device 200 .

[0169] refer to Figure 13 to Figure 14 In some embodiments, the electrospinning film stacking device 200 includes:

[0170] The first component 210 has a receiving groove on the top;

[0171] A third annular member 230, whose outer diameter is smaller than the inner circumferential dimension of the receiving groove; the third annular member 230 is configured to be disposed in the receiving groove;

[0172] The electrospun film fixture 100 in the above embodiment; the electrospun film unit formed by the fixture 100 is configured to be placed on top of the first component 210, and the circumferential dimension of the electrospun film unit is larger than the circumferential dimension of the first component 210;

[0173] The circumferential dimension of the second component 220 is smaller than the inner circumferential dimension of the containing groove; the second component 220 is configured to abut against the electrospinning film unit from the top of the electrospinning film unit;

[0174] The fifth annular member 250 has an inner diameter greater than the circumferential dimensions of the second member 220 and the first member 210; the fifth annular member 250 is configured to be sleeved on the second member 220 and press the film unit so that the outer edge of the film unit is circumferentially wrapped around the outer circumference of the first member 210; and

[0175] The fourth annular member 240 has an outer diameter smaller than the inner diameter of the third annular member 230; the fourth annular member 240 is configured to be stacked in sequence on a plurality of film units, and after the outer edges of the plurality of film units are successively wrapped around the outer circumference of the first member 210 in sequence, it is arranged on the inner side of the third annular member 230 and clamped with the third annular member 230, and the third annular member 230 and the fourth annular member 240 cooperate to fix the stacked plurality of film units.

[0176] refer to Fig.14 The operation steps of the electrospinning film stacking device 200 are as follows:

[0177] S1: placing the third annular member 230 in the top receiving groove of the first member 210;

[0178] S2 placing the electrospun film unit obtained by the electrospun film fixing device 100 on the top of the first component 210;

[0179] S3: placing the second component 220 in contact with the membrane unit from the top of the membrane unit;

[0180] S4: sleeve the fifth annular member 250 onto the second component 220 and press the circumferential edge of the film unit so that the outer edge of the film unit is circumferentially wrapped around the top periphery of the first component 210, and remove the second component 220 and the electrospinning film fixing device 100;

[0181] S5: removing the fifth annular member 250, and repeating steps S2 to S4 until the number of stacked membrane units meets the requirement;

[0182] S6: The fourth annular member 240 is disposed on the inner side of the top of the third annular member 230 and is clamped with the third annular member 230. The third annular member 230 and the fourth annular member 240 cooperate to fix the stacked multiple film units.

[0183] In some embodiments, the electrospinning film preparation system includes an electrospinning film winding device 300 .

[0184] refer to Figures 15 to 18 In some embodiments, the electrospinning film winding device 300 includes:

[0185] Strip plate 310;

[0186] The electrospun film fixing device 100 in the above embodiment; the fixing device 100 is configured to form a strip-shaped electrospun film unit, and the electrospun film unit is configured to be attached to the top surface of the strip plate 310; and

[0187] The film cutting member 320 is configured to disconnect the film unit from the fixing device along the length direction of the film unit, and the film unit is automatically rolled into a roll.

[0188] refer to Fig.15 and Fig.16 In some embodiments, the film breaking member 320 includes:

[0189] A first fixing block 321;

[0190] A second fixing block 322; and

[0191] There are two shearing pieces 323, each of which includes a first blade 3231 and a second blade 3232. The first blades 3231 of the two shearing pieces 323 are fixedly arranged between the first fixed block 321 and the second fixed block 322 at intervals; the two shearing pieces 323 are configured to simultaneously cut off the connection between the film unit and the fixing device along the length direction of the film unit.

[0192] refer to Fig.15 In the first embodiment, the operation method of the electrospinning film winding device 300 is:

[0193] A square film unit formed by the electrospinning film fixture 100 is prepared;

[0194] The film cutting member 320 is operated so that its two shearing members 323 simultaneously cut the connection between the film unit and the fixing device along the length direction of the film unit, and the film unit is naturally wound up with the shearing of the shearing members 323 to form a roll without a reel.

[0195] refer to Fig.17 In some embodiments, the film breaking member 320 includes:

[0196] A first main body member 324;

[0197] A first side member 325;

[0198] A second side member 326; and

[0199] Two blades 327, one blade 327 is fixed to the first side of the first main body 324 through the first side piece 325, and the other blade 327 is fixed to the second side of the first main body 324 through the second side piece 326; the two blades 327 are configured to simultaneously cut off the connection between the film unit and the fixing device along the length direction of the film unit.

[0200] refer to Fig.18 In some embodiments, the electrospinning film winding device 300 further includes a reel 330 , which is configured to be disposed at an end of the film unit so that the film unit is wound around the reel 330 .

[0201] refer to Fig.18 In some embodiments, the electrospinning film winding device 300 further includes a strip plate 310 , and the strip plate 310 is configured to support below the square film unit formed by the electrospinning film fixture 100 .

[0202] refer to Fig.18 In some embodiments, a recess 311 is provided at the end of the strip plate 310 , and the recess 311 is used to accommodate the reel 330 .

[0203] refer to Fig.18 In the second embodiment, the operation method of the electrospinning film winding device 300 is:

[0204] Place the square film unit formed by the electrospinning film fixture 100 on the strip plate 310;

[0205] The film cutting member 320 is operated so that its two blades 327 simultaneously cut the connection between the film unit and the fixing device along the length direction of the film unit.

[0206] Place the reel 330 in the recess 311 of the strip plate 310 and fix the reel 330 to the end of the film unit;

[0207] The reel 330 is rotated to roll the strip-shaped film unit into a roll shape having the reel 330 .

[0208] In some embodiments, the electrospinning film preparation system includes an electrospinning film roll fixing device 900 .

[0209] See also Fig.19 In some embodiments, the electrospinning film roll fixing device 900 includes a third fixing member 910 and a fourth fixing member 920 .

[0210] Two grooves are arranged at intervals on the third fixing member 910, and there are two fourth fixing members 920. Buckles are arranged at the ends of the two fourth fixing members 920. The electrospinning film roll is placed between the two grooves of the third fixing member 910. The buckles of the two fourth fixing members 920 press part of the edge of the electrospinning film roll and fix it in the two grooves. The electrospinning film roll is fixed between the two fourth fixing members 920.

[0211] In some embodiments, the electrospinning film preparation system includes an electrospinning film preparation device 400 .

[0212] refer to Figures 1 to 3 In some embodiments, the electrospinning film preparation device 400 includes a power source 410, a collector 420, a jet element 430 and the electrospinning film fixing device in the above embodiments.

[0213] The wire collector 420 is configured to rotate. The wire collector 420 includes:

[0214] At least two rods 421 connected to the negative pole of the power source 410 or to the ground;

[0215] A first connecting member 422 connected to the first ends of at least two rods 421; and

[0216] A second connecting member 423 connected to the second ends of at least two rods 421;

[0217] The spraying member 430 is connected to the positive electrode of the power source 410; the spraying member 430 is configured to spray the solution toward the collector 420 to form an electrospinning film on at least two rods 421; and

[0218] The electrospun film fixing device is configured to fix the electrospun film.

[0219] refer to Figure 1In some embodiments, the electrospinning film preparation device 400 further includes a guide rail 440 , and the injection member 430 is movably disposed on the guide rail 440 , and an extension direction of the guide rail 440 is parallel to an extension direction of at least two rods 421 .

[0220] In some embodiments, the electrospinning film preparation system includes a first test component 500 .

[0221] refer to Fig. 20 In some embodiments, the first test assembly 500 includes a first frame 510 and two clamping members 520 .

[0222] The first frame 510 includes a first frame bar 511 and a second frame bar 512 that are oppositely disposed, and a third frame bar 513 and a fourth frame bar 514 that are oppositely disposed.

[0223] The first frame bar 511 and the second frame bar 512 are respectively provided with a first breakable portion 530. Two clamping members 520 are respectively provided on the third frame bar 513 and the fourth frame bar 514 in a one-to-one correspondence to clamp the electrospun film, and the first breakable portion 530 is configured to be broken to perform a tensile test on the electrospun film.

[0224] In some embodiments, the electrospinning film preparation system includes a second test component 600 .

[0225] refer to Fig.21 In some embodiments, the second test assembly 600 includes two second frames 610, and a second breakable portion 620 is respectively provided on one of the opposite sides of the two second frames 610. The two second frames 610 are configured to clamp the electrospun film and immersed in nitrogen liquid. The second breakable portions 620 of the two second frames 610 are configured to break so as to perform a brittle fracture test on the electrospun film.

[0226] In some embodiments, the electrospinning film preparation system includes a third test component 700 .

[0227] refer to Fig. 22 In some embodiments, the third test component 700 includes a plate 710 and a conductive adhesive 720. The plate 710 is provided with a boss 711. The conductive adhesive 720 is configured to press a recess through the boss 711. The conductive adhesive 720 is configured to carry an electrospun film to perform an electron microscope scanning test on the electrospun film.

[0228] In some embodiments, the third test assembly 700 further includes a pressing piece 730 , on which the conductive adhesive 720 is disposed, and the pressing piece 730 carries the conductive adhesive 720 to press a recess on the boss 711 of the plate 710 .

[0229] In some embodiments, the electrospinning film preparation system includes a fourth test component 800 .

[0230] refer to Fig.23 In some embodiments, the fourth test component 800 includes a sixth ring-shaped member 810 and a seventh ring-shaped member 820, the outer diameter of the sixth ring-shaped member 810 is smaller than the inner diameter of the seventh ring-shaped member 820, the sixth ring-shaped member 810 is arranged on the first side of the electrospun film, and the seventh ring-shaped member 820 is configured to be arranged on the second side of the electrospun film, the sixth ring-shaped member 810 is configured to be arranged in the seventh ring-shaped member 820 and protrude from the seventh ring-shaped member 820, the sixth ring-shaped member 810 is configured to clamp the electrospun film with the seventh ring-shaped member 820, and the portion of the sixth ring-shaped member 810 protruding from the seventh ring-shaped member 820 is covered with the electrospun film, so as to perform a hydrophilicity and hydrophobicity test on the electrospun film.

[0231] The following is combined with Figures 1 to 25 Some specific embodiments of the electrospinning film preparation system are described.

[0232] The electrospinning film preparation system includes a complete set of devices for preparation, characterization and operation of electrospinning films. Specifically, it includes two types of collectors, a circular film fixture, a rectangular film fixture, a characterization device (including a first test component for testing the mechanical properties of electrospinning films by stretching, a second test component for liquid nitrogen brittle fracture test of electrospinning films, a third test component for scanning electron microscope electrospinning film sample preparation, and a fourth test component for hydrophilicity and hydrophobicity test of electrospinning films), a stacking device, a winding device, and an electrospinning film roll fixture.

[0233] The electrospinning film preparation provided by the embodiment of the present invention includes two types of collectors: a rectangular collector 420 for preparing a large-area electrospinning film (see Figure 3 ) and a prismatic collector 420 for efficiently preparing electrospinning films (see Figure 1 and Figure 2 ). The length and width of the rectangular collector 420 can be modified relatively flexibly to improve the fiber orientation, the collection area (collection amount), the spinning efficiency, etc. The dynamic and static connection method between the collector and the grounding wire needs to be connected through the inner and outer rings of the bearing: the metal rod of the collector is connected to the metal part of the inner ring of the bearing with a wire, and then the metal part of the outer ring of the bearing is connected to the grounding wire (or negative pressure) with a wire. The rotation speed of the collector 420 needs to match the jet speed; the direction (clockwise / counterclockwise) needs to be determined according to the collection situation.

[0234] The electrospinning film preparation device also includes an ejector 430, which needs to move parallel to the axis of the collector 420 during spinning, and the moving speed, moving range and relative position of the collector 420 need to be within a certain range.

[0235] When a large rectangular collector 420 is used in conjunction with spinning, the jet needs to reach an ultra-stable spinning state (there is almost no jet spinning phenomenon): for this reason, the viscosity of the spinning material is relatively large, and the voltage, needle size, distance between the needle and the collector 420, and injection pump push speed all need to be within a certain range.

[0236] In some embodiments, polylactic acid-co-glycolic acid copolymer and hexafluoroisopropanol are mixed to obtain a solution with a concentration of 10%, and the following spinning parameters are used: a prismatic collector 420 is used, and a negative voltage of -2kV is applied, a positive voltage of +12kV is applied to the needle of the injection member 430, the distance between the needle tip of the injection member 430 and the metal rod of the collector is 5cm, the injection member 430 moves parallel to 18cm, the center of the injection member 430 is aligned with the center of the collector, the feeding speed of the injection member 430 is 0.1mm / min, the rotation speed of the prismatic collector 420 is 700RPM, and after collecting for 1h, the collector 420 is turned around and collected for another 1h. After the spinning is completed, the collector 420 is removed.

[0237] In some embodiments, the electrospinning film preparation process includes a preparation process for preparing batches of uniform, uniform thickness, uniform filament spacing, large area, oriented electrospinning films: including optimization and limitation of spinning materials, equipment and operating procedures.

[0238] (1) The electrospinning film preparation device needs to use the following parameter ranges during spinning:

[0239] ① The spinning material includes at least one of polylactic acid-glycolic acid (PLGA), polylactic acid (PLA), and polycaprolactone (PCL), and the above material is dissolved in at least one of hexafluoroisopropanol, dichloromethane, and acetic acid aqueous solution.

[0240] ② Voltage: The wire collector 420 is grounded or applies a voltage in the range of 0 to -5 kV; the needle of the injection member 430 applies a voltage in the range of +5 to +15 kV.

[0241] ③ The needle size of the injection piece 430 is: 13G~25G.

[0242] ④ The distance between the needle and the wire collector 420: 3cm to 15cm.

[0243] ⑤ Injection part 430 push speed: 0.5mL / h~1.5mL / h.

[0244] ⑥ The rotation speed of the wire collector 420: 200RPM~1000RPM.

[0245] (2) The ejector 430 needs to reciprocate along the axis of the collector 420 while spinning. The moving speed is 1 cm / min to 20 cm / min; the moving range is from 4 cm shorter than the span of the collector 420 to 5 cm longer than the span of the collector 420; the midpoint of the moving range is 0 to 5 cm eccentric to the center of the collector 420 (eccentric away from the negative voltage terminal).

[0246] (3) Receiving time: Depending on the size of the collector 420 and the required film fiber spacing, the receiving time is 0.5 h to 3 h.

[0247] (4) Fixed spinning: Use a fixing device to fix the electrospun film from the collector 420 and remove the spinning. Maintain the integrity of the entire electrospun film material, and cut the film unit formed by the fixing device after the inactivation treatment.

[0248] (5) Inactivation: For materials that shrink when exposed to alcohol, such as PLGA, it is recommended to use ethylene oxide to inactivate the electrospun film after it is fixed with a film fixing device. The temperature range is 35℃~45℃, the relative humidity is 40%~80%, and the inactivation time is 2h~5h.

[0249] Advantages of the collector 420: The rotary collector 420 of the present invention has the potential for large-scale production of electrospun films. Secondly, the rotary collector 420 can continuously collect oriented spinning for up to 3 hours, so as to adjust the fiber spacing (film thickness), while the notched collector in the related art cannot stably collect oriented spinning for a long time, so it is necessary to manually fix the spinning repeatedly with a rectangular frame to control the fiber spacing (adjust the film thickness). In addition, the use of the rotary collector 420 in conjunction with the ultra-stable spinning state can spin fibers with diameters ranging from hundreds of nanometers to several microns.

[0250] The electrospinning film fixture 100 is used to remove the electrospinning film from the collector 420 in situ. The electrospinning film fixture 100 can not only improve the collection efficiency, but also ensure the collection quality (the in-situ collection will not destroy the microscopic morphology or orientation of the fiber). In order not to damage the electrospinning film, the electrospinning film fixture 100 needs to be inactivated after collecting the wire, and inactivated in ethylene oxide with a specific range of temperature, humidity and duration. After the inactivation treatment, the electrospinning film between the fixtures is cut to obtain a separate electrospinning film unit.

[0251] In some embodiments, the electrospun film fixture 100 includes a circular film fixture, which is used to fix the electrospun film from the collector 420 to the fixture in situ, so that the electrospun film maintains the macroscopic and microscopic morphology of the fiber during subsequent use and operation. This circular film fixture can be made into three sizes to adapt to three common cell culture well plates (6-well plate, 12-well plate and 24-well plate).

[0252] Each set of fixing devices 100 includes a first annular member 112, a second annular member 121, a first plate 114, a second plate 123 and a third plate 130. The first annular member 112 and the second annular member 121 are assembled by buckles and elastic deformation of the annular portion of the main body, and the electrospinning film is fixed by the interference fit between the outer surface of the second annular member 121 and the inner surface of the first annular member 112.

[0253] The first annular member 112 and the second annular member 121 are assembled into a fixing fixture kit through the first buckle 122 of the second annular member 121 and the first groove 113 of the first annular member 112. The first protrusion 125 of the second annular member 121 is positioned and assembled with the through hole 127 on the second plate 123. The first groove 113 of the first annular member 112 is positioned and assembled with the second buckle 1151 on the first plate 114. The positioning hole 1141 on the first plate 114 is positioned and assembled with the positioning column 124 on the second plate 123. The second protrusion 131 of the third plate 130 is pressed to be positioned and assembled through the through hole 127 on the second plate 123, and the first protrusion 125 of the second annular member 121 is pressed through the third plate 130 to assemble the first annular member 112 and the second annular member 121.

[0254] After the first annular member 112 and the second annular member 121 are assembled, the bottom surfaces of the first annular member 112 and the second annular member 121 are flush or the bottom surface of the second annular member 121 is slightly lower than the bottom surface of the first annular member 112, so that the fixed electrospun film is in contact with the culture dish to ensure efficient and uniform planting of cells.

[0255] After the cell planting is completed, the first annular member 112 and the second annular member 121 are turned over, so that the electrospun film is separated from the bottom of the orifice plate and suspended in the culture medium to avoid physical damage caused by the contact of the bottom of the orifice plate with the cell or the electrospun film during the long-term culture process. And after turning over the first annular member 112 and the second annular member 121, another cell can be planted again. The first annular member 112 and the second annular member 121 can be separated by twisting to reuse the fixture set. The first annular member 112 and the second annular member 121 can be assembled with the first plate 114 and the second plate 123 respectively, and the first plate 114 and the second plate 123 can be assembled with the wire collector 420 to position the first annular member 112 and the second annular member 121, and facilitate the assembly of the first annular member 112 and the second annular member 121. The third plate 130 can be assembled with the second plate 123 to assemble a plurality of first annular members 112 and the second annular member 121 in a disposable manner.

[0256] refer to Figure 7 In some embodiments, the process flow of fixing the electrospun film by the prismatic collector 420 with a circular fixture suitable for a 6-well plate is as follows:

[0257] The second annular member 121 of the circular fixture for the 6-well plate is assembled into the second groove 126 on the second plate 123. The first annular member 112 of the circular fixture for the 6-well plate is assembled into the first groove 115 on the first plate 114.

[0258] The electrospun film on one side of the prismatic collector 420 is placed on the first plate 114 .

[0259] The wire collector 420 and the first plate 114 are turned upside down together.

[0260] The second plate 123 is positioned and assembled with the first plate 114 from below the electrospinning film through the positioning holes 1141 on the first plate 114 and the positioning posts 124 on the second plate 123 .

[0261] Turn the entire assembly upside down.

[0262] Assemble the third plate 130 and the second plate 127, press the third plate 130, insert the second protrusion 131 on the third plate 130 into the through hole 127 on the second plate 123, and push the first protrusion 125 with the second annular member 121 and the first annular member 112 to clamp and fix the electrospinning film.

[0263] The third plate 130 , the first plate 114 and the second plate 123 are disassembled, and the fixing fixture set formed by the film and the first annular member 112 and the second annular member 121 is removed, and 9 electrospinning film units adapted to the 6-well plate are obtained by cutting.

[0264] The circular film fixture can avoid the complicated operation of hanging wire collection through the space limitation of the first plate 114 and the second plate 123. The assembly of the fixture is snap-on assembly through elastic deformation between parts. There is only one direction of pressing in the assembly process, and no additional device is required, which avoids the rotation operation to ensure the integrity of the microscopic morphology of the electrospun film. The first annular member 112 and the second annular member 121 can be manually and quickly disassembled by rotating the assembly without additional devices, and the fixture can be reused. After fixation, the electrospun film is located at the bottom of the second annular member 121, which can facilitate efficient and uniform planting of cells.

[0265] In some embodiments, the electrospinning film fixture 100 comprises a rectangular film fixture (see Figures 8 to 12 ) is used to fix the electrospun film from the collector 420 to the fixture in situ to obtain a large-area rectangular electrospun film unit, so that the electrospun film can maintain the macroscopic and microscopic morphology of the fiber during subsequent use and operation.

[0266] The rectangular film fixing device includes a rectangular first fixing member 110 and a strip member 128. The rectangular first fixing member 110 determines the area of ​​the electrospinning film, and the maximum area can be adapted to a general square culture dish (13cm×13cm). The length of the rectangular first fixing member 110 ranges from 0.5cm to 12cm.

[0267] The rectangular first fixing member 110 is a rectangular frame, and two strip partitions 116 are formed on the rectangular frame, and a second slot 117 is provided on both sides of each strip partition 116. A third buckle 129 of an asymmetric structure is provided on the strip member 128. The strip member 128 is connected to the first fixing member 110 by the third buckle 129 being clamped in the second slot 117. A strip member 128 is provided in each second slot 117. A convex portion 118 protruding upward is provided on the side of the second slot 117, and the convex portion 118 abuts against the spinning film, so that after the rectangular fixing device is flipped, the spinning film can protrude from the rectangular first fixing member 110, which is conducive to the electrospinning film being attached to the bottom of the culture dish.

[0268] The rectangular first fixing member 110 and the strip member 128 respectively have a second card slot 117 and a third card buckle 129 of asymmetric shape. The assembly of the asymmetric card buckle is similar to the process of wearing shoes. No additional device is required. It is an insertion and rotation movement, so that the fracture of the spinning on one side is sacrificed (with a certain probability) during the clamping process to ensure the integrity of the spinning on the other side (functional side). In the case where the span of the strip partition 116 is large, a support column is required in the middle to increase the rigidity of the fixture and reduce the deformation caused by the shrinkage of the oriented spinning. The side of the second card slot 117 is provided with a convex portion 118 protruding upward, and the convex portion 118 abuts the spinning film. After the rectangular fixing device is flipped, the spinning film protrudes from the rectangular first fixing member 110, which is conducive to the electrospinning film being attached to the bottom of the culture dish, ensuring efficient and uniform planting of cells. After the cell planting is completed, the fixing device is flipped so that the electrospinning film is suspended in the culture solution from the bottom of the orifice plate to avoid physical damage caused by the contact of the bottom of the orifice plate with the cells or the electrospinning film during the long-term culture process.

[0269] refer to Fig.12 The process flow of fixing the electrospun film on the prismatic collector 420 with a rectangular fixture is as follows:

[0270] The electrospun film on one side of the prismatic collector 420 is placed on the rectangular first fixing member 110 .

[0271] Two strip members 128 are respectively assembled on both outer sides of the length direction of the rectangular first fixing member 110. The top member is assembled obliquely, so that it is similar to the action of "putting on shoes" to reduce the damage of the functional end fiber.

[0272] The electrospun film is cut from the collector 420 .

[0273] Two strip members 128 are assembled on both inner sides of the rectangular first fixing member 110 in the length direction in the same assembly manner.

[0274] The electrospun film fixing device 100 provided in the embodiment of the present invention can fix the oriented electrospun film firmly and for a long time, and can be used repeatedly without the need for additional plasma surface treatment equipment.

[0275] The electrospun film preparation system provided in an embodiment of the present invention includes a characterization device, including a first test component for testing the mechanical properties of the electrospun film by stretching it, a second test component for performing a liquid nitrogen brittle fracture test on the electrospun film, a third test component for scanning electron microscopy (SEM) electrospun film sample preparation, and a fourth test component for performing a hydrophilicity and hydrophobicity test on the electrospun film, which can respectively assist in characterization experiments on the mechanical properties, brittle fracture (observation of the cross section), SEM sample preparation and hydrophilicity and hydrophobicity of the electrospun film.

[0276] refer to Fig. 20 , the first test assembly 500 is similar to a rectangular fixture.

[0277] The first test assembly 500 includes a first frame 510 and two clamps 520 .

[0278] The first frame 510 includes a first frame bar 511 and a second frame bar 512 that are oppositely disposed, and a third frame bar 513 and a fourth frame bar 514 that are oppositely disposed.

[0279] The first frame bar 511 and the second frame bar 512 are respectively provided with a first breakable portion 530. Two clamping members 520 are respectively provided on the third frame bar 513 and the fourth frame bar 514 in a one-to-one correspondence to clamp the electrospun film, and the first breakable portion 530 is configured to be broken to perform a tensile test on the electrospun film.

[0280] By using the first test assembly 500, the area of ​​the fixed electrospun film can be adjusted arbitrarily according to the tensile test standards of different materials. The cross-sections of the relatively arranged first frame strip 511 and the second frame strip 512 become narrower in the middle, which is convenient for cutting before the tensile test.

[0281] refer to Fig.21 In some embodiments, the second test assembly 600 includes two second frames 610, and a second breakable portion 620 is respectively provided on one of the opposite sides of the two second frames 610. The two second frames 610 are configured to clamp the electrospun film and immersed in nitrogen liquid. The second breakable portions 620 of the two second frames 610 are configured to break so as to perform a brittle fracture test on the electrospun film.

[0282] The electrospun film is too thin to be broken, and the cross-sectional morphology of the film is not easy to observe. The second test assembly 600 can clamp the electrospun film in situ, and keep the microscopic morphology of the fiber during the liquid nitrogen breaking. The cross-section of one of the two second frames 610 on the opposite sides is narrowed at the center to facilitate the breaking operation.

[0283] refer to Fig. 22 In some embodiments, the third test component 700 includes a plate 710 and a conductive adhesive 720. The plate 710 is provided with a boss 711. The conductive adhesive 720 is configured to press a recess through the boss 711. The conductive adhesive 720 is configured to carry an electrospun film to perform an electron microscope scanning test on the electrospun film.

[0284] The electrospun film is too thin and is easily embedded in the conductive glue during SEM sample preparation, making it difficult to observe. The indentation boss 711 of the third test component 700 can press the conductive glue into a "concave" shape, so that a "bridge"-shaped observation area is formed after the electrospun film is attached. The fibers in this area are suspended above the conductive glue, making SEM observation clear.

[0285] refer to Fig.23 In some embodiments, the fourth test component 800 includes a sixth ring-shaped member 810 and a seventh ring-shaped member 820, the outer diameter of the sixth ring-shaped member 810 is smaller than the inner diameter of the seventh ring-shaped member 820, the sixth ring-shaped member 810 is arranged on the first side of the electrospun film, and the seventh ring-shaped member 820 is configured to be arranged on the second side of the electrospun film, the sixth ring-shaped member 810 is configured to be arranged in the seventh ring-shaped member 820 and protrude from the seventh ring-shaped member 820, the sixth ring-shaped member 810 is configured to clamp the electrospun film with the seventh ring-shaped member 820, and the portion of the sixth ring-shaped member 810 protruding from the seventh ring-shaped member 820 is covered with the electrospun film, so as to perform a hydrophilicity and hydrophobicity test on the electrospun film.

[0286] The fourth test assembly 800 is similar to a circular fixing device. After assembly, the bottom surface of the sixth ring 810 is lower than the bottom surface of the seventh ring 820, so that the fixed electrospun film protrudes, and after turning over, it is convenient for horizontal photography during the hydrophilicity and hydrophobicity test.

[0287] According to the above embodiments, the characterization fixture can take samples in situ and maintain the corresponding characteristics of the electrospun film: for example, the first test component used for the tensile test can maintain the original length of the electrospun film until the first test component can be cut before the tensile test begins; the third test component used for SEM sample preparation can press the conductive glue to create a "bridge" structure, so that the electrospun film adhered to it is suspended in the air, avoiding the electrospun film from sinking into the conductive glue and affecting the observation quality; the fourth test component used for hydrophilicity and hydrophobicity testing allows the fixed electrospun film to protrude, facilitating the observation of droplets during hydrophilicity and hydrophobicity characterization.

[0288] refer to Fig.13 and Fig.14 In some embodiments, the electrospinning film preparation system includes an electrospinning film stacking device 200, which can be used in conjunction with a circular film fixture suitable for a 6-well plate and a 24-well plate to stack multiple layers of electrospinning films on the circular film fixture suitable for a 6-well plate and fix them on a circular film fixture suitable for a 24-well plate.

[0289] The electrospinning film stacking device 200 includes a first component 210 , a second component 220 , a third annular component 230 , a fourth annular component 240 and a fifth annular component 250 .

[0290] The fourth annular member 240 can be assembled with the third annular member 230 of the circular fixture suitable for the 24-well plate, and assembled with the open circular ring to temporarily fix the electrospun film to the first component 210. The second component 220 is assembled with the first component 210 to press the electrospun film so as to maintain the microscopic morphology of the electrospun film during the stacking operation. The first component 210 and the second component 220 both have a spinning orientation indicator structure for aligning the fibers between the layers.

[0291] By adjusting the matching size of the circular fixture for 24-well plates, the number of stacking layers can reach 50. Special design of fixtures for 24-well plates is required, and large-scale preparation of fixtures for 6-well plates and spinning, dyeing, alternating stacking, and cross-sectional photography are required.

[0292] refer to Fig.14 The process flow of stacking electrospinning films using the electrospinning film stacking device 200 is as follows:

[0293] S1 places the third annular member 230 in the top receiving groove of the first component 210 .

[0294] S2: placing the film unit obtained by the electrospinning film fixture 100 on the top of the first component 210, so that the fiber orientation is perpendicular to the line connecting the two slots of the fixture.

[0295] S3: the second component 220 is brought into contact with the film unit from the top thereof; the electrospun film is pressed to maintain the microscopic morphology of the electrospun film during the stacking operation.

[0296] S4: sleeve the fifth annular member 250 onto the second component 220 and press the circumferential edge of the film unit so that the outer edge of the film unit is circumferentially wrapped around the top periphery of the first component 210, and remove the second component 220 and the electrospinning film fixing device 100.

[0297] S5: removing the fifth annular member 250, and repeating steps S2 to S4 until the number of stacked membrane units meets the requirement.

[0298] S6: The fourth annular member 240 is disposed on the inner side of the top of the third annular member 230 and is clamped with the third annular member 230. The third annular member 230 and the fourth annular member 240 cooperate to fix the stacked multiple film units.

[0299] The stacked multi-layer electrospun film assembly fixed by the third annular member 230 and the fourth annular member 240 is taken out from the first component 210 of the stacking device, and the excess electrospun film is cut off.

[0300] The electrospinning film stacking device 200 provided in the embodiment of the present invention can be used in conjunction with a circular film fixture for a 6-well plate and a circular film fixture for a 24-well plate, and is used to stack and fix the electrospinning film received on the circular film fixture for a 6-well plate on the circular film fixture for a 24-well plate. By adjusting the matching size of the circular fixture for a 24-well plate, the number of stacked layers can reach 50, and the resolution of the stacked 3D material in the stacking direction can be as high as 10 microns.

[0301] refer to Figures 15 to 18 In some embodiments, the electrospinning film preparation system includes an electrospinning film winding device 300 .

[0302] The electrospinning film winding device 300 includes a Venus scissors clamping device, a razor clamping device and a winding auxiliary strip plate 310. The clamping device clamps two Venus scissors or two razor blades at the same time, and the spacing between them is the same width as the electrospinning film fixed by the rectangular fixing device. When used in conjunction with the rectangular fixing device, the electrospinning film can be accurately and efficiently cut. The winding auxiliary strip plate 310 and the rectangular film fixing device are used in conjunction with each other to maintain the flatness of the cut electrospinning film through surface tension, locate the position of the reel 330, and assist the reel 330 and the electrospinning film in sticking at the slot. The electrospinning film winding device can roll an electrospinning film without a reel 330, and the reel will automatically form a reel shape through its surface tension and / or internal stress; or by laminating the reel 330 (prepared separately) and the electrospinning film attached to the auxiliary strip plate 310, it is rolled into a reel with a reel 330.

[0303] refer to Fig.16 In the first embodiment, the film cutting member 320 includes a first fixing block 321, a second fixing block 322 and two shearing members 323. The shearing members 323 include Venus shears.

[0304] refer to Fig.17 In the second embodiment, the film cutting member 320 includes a first main body member 324, a first side member 325, a second side member 326 and two blades 327. The blades 327 include razors.

[0305] The electrospinning film winding device 300 further includes a strip plate 310, which is configured to be supported below the square film unit formed by the electrospinning film fixing device 100. A recess 311 is provided at the end of the strip plate 310, and the recess 311 is used to accommodate the reel 330 to prevent the reel 330 from accidentally sticking to the surface of the strip plate 310 when attaching the electrospinning film.

[0306] refer to Fig.15The process flow of using a 330mm non-reel winding device in conjunction with a rectangular fixing device to roll a 330mm non-reel electrospinning film roll is as follows:

[0307] The two Venus scissors are fixed by the first fixing block 321 and the second fixing block 322 .

[0308] Soak the electrospun film with water and cut the two ends of the roll film according to the required length (i.e. determine the starting and ending positions of the electrospun film roll).

[0309] The film cutter 320 is used to slowly cut off one end of the electrospun film on the rectangular film fixture from one end of the film in a direction perpendicular to the orientation direction of the electrospun film fibers. The feed speed is less than 2 mm per cut. The electrospun film will be automatically rolled into a roll until the electrospun film is completely cut off. The electrospun film roll fixture is used to clamp the electrospun film roll for subsequent use.

[0310] refer to Fig.18 The process flow of winding the electrospun film with a reel 330 using a reel winding device in combination with a rectangular fixing device is as follows:

[0311] Required devices: Reel 330: Print polylactic acid (PLA filament, about 250um in diameter) using an Ultimaker 3D printer, and cut off a filament about 2 cm long. Assemble two razor blades with a blade clamping device (first main body 324, first side piece 325, second side piece 326).

[0312] Soak the electrospun film with water, cut the two ends of the film according to the required length, and place the electrospun film on the strip plate 310 so that one end of the film is aligned with one end of the strip plate 310.

[0313] The electrospun film is cut by two razor blades of the film cutting member 320 .

[0314] The electrospun film is attached to the strip plate 310 by means of surface tension.

[0315] A 502 all-purpose glue of about 5 mm in length is applied to the middle of the PLA filament, and the filament is adhered to the electrospun film at the concave portion 311 of the strip plate 310 .

[0316] The PLA filaments are pushed away from the strip plate 310 by both hands and the electrospun film is wound up. The electrospun film roll is clamped by an electrospun film roll fixing device for subsequent use.

[0317] In some embodiments, the electrospinning film preparation system includes an electrospinning film roll fixture 900, which is used in conjunction with a rectangular film fixture to clamp the electrospinning film roll to maintain its macroscopic and microscopic structure, or to continue culturing cells on the electrospinning film after the winding operation to form a roll-type biological tissue.

[0318] See also Fig.19 In some embodiments, the electrospinning film roll fixing device 900 includes a third fixing member 910 and a fourth fixing member 920. The third fixing member 910 is provided with two grooves at intervals, and the number of the fourth fixing members 920 is two. The ends of the two fourth fixing members 920 are provided with buckles, and are assembled by asymmetric buckles similar to rectangular clamps. The electrospinning film roll is placed between the two grooves of the third fixing member 910, and the buckles of the two fourth fixing members 920 press part of the edge of the electrospinning film roll and fix it in the two grooves, and the electrospinning film roll is fixed between the two fourth fixing members 920.

[0319] The electrospinning film preparation system provided in the embodiment of the present invention can significantly improve the quality of the prepared electrospinning film, ensuring both the microscopic fiber structure and maintaining the macroscopic morphology and size, which can greatly simplify the operational difficulty during preparation, characterization and operation, significantly improve production efficiency, and reduce characterization and detection errors.

[0320] Based on the description of the above embodiments, the embodiments of the present invention have at least the following beneficial effects:

[0321] The present invention proposes a device and a process for preparing, characterizing and stacking electrospinning film materials, and describes its application in cell culture. The method can be used to prepare large-area, uniform, and oriented electrospinning films. The preparation and characterization methods are simpler and more efficient than related technologies, and can achieve the application of double-sided cell seeding. The device provided in the embodiment of the present invention is suitable for common electrospinning materials, such as PLGA, PLA, gelatin, PCL, collagen, and mixtures thereof.

[0322] The fixing device, the characterization device (excluding the brittle fracture test assembly), the stacking device, the shearing device and the roll fixing device of the present invention can be reused.

[0323] The electrospun film prepared by the device of the present invention can achieve cell penetration of the scaffold material in a short time (within 10 minutes) when inoculating cells, so that the cells are evenly distributed, which is beneficial to the interaction between cells, thereby improving the homogeneity of the cultured tissue. The 3D electrospun material formed by the multi-layer electrospun film stack prepared by the device of the present invention and the 3D electrospun material formed by the single-layer electrospun film roll can be used to culture biological tissues to overcome the problem that cells are difficult to penetrate the oriented electrospun material, so that the cultured tissue is highly cellularized.

[0324] The 3D electrospun material prepared by stacking multiple layers of electrospun films can achieve a gradient change in the properties of each layer of film in the stacking direction with a resolution of 10 microns and a total thickness of millimeters.

[0325] refer to Fig.24 , Cross-section of a scaffold material with 20 layers of electrospun films. The delamination of some of the electrospun films can be clearly seen (white dashed lines).

[0326] The 3D stent material wound with a reel 330 formed by the electrospinning film winding device prepared by the invention can accurately simulate the Archimedean spiral (the winding diameter error is about 3%).

[0327] refer to Fig.25 , a 3D scaffold material with a reel 330 is formed by winding a 10 cm long electrospun film with PLA filaments as reels 330 using a winding device. (A) Theoretically, the same size of Archimedean spiral: the film is 10 cm long, 10 μm thick, and the diameter of the reel 330 is 210 μm, which should result in a reel with a diameter of 1.15 mm. (B) The actual reel is 1.12 mm in diameter.

[0328] The skeletal muscle cells prepared using this method have high fusion and can reach a certain thickness to form a 3D structure, breaking through the 2D limitations of oriented electrospinning to culture skeletal muscle cells.

[0329] The present invention can provide a complete set of devices and solutions for the preparation, characterization and operation (including stacking and winding) of electrospinning films with a thickness of less than 20 microns.

[0330] The preparation process of the electrostatic spinning film of the present invention comprises materials, devices and operation procedures for preparing batches of uniform electrostatic spinning films with uniform thickness, consistent filament spacing, large area and orientation.

[0331] The materials of the device of the present invention are all polymer materials for 3D printing. However, other materials can be used if they have good physical and chemical compatibility with the spun material, that is, they do not destroy the fiber morphology or chemical composition and will not affect the function. Including but not limited to metal materials, aluminum and its alloys, iron and its alloys, polymer materials, etc.

[0332] The kit components and assembly process of the present invention have the potential for transformation into automated mechanical assembly, which can further improve the efficiency and consistency of wire collection.

[0333] Based on the above-mentioned embodiments of the present invention, in the absence of explicit negation or conflict, the technical features of one embodiment may be beneficially combined with one or more other embodiments.

[0334] Although some specific embodiments of the present invention have been described in detail by way of examples, it should be understood by those skilled in the art that the above examples are for illustration only and are not intended to limit the scope of the present invention. It should be understood by those skilled in the art that the above embodiments may be modified or some technical features may be replaced by equivalents without departing from the scope and spirit of the present invention. The scope of the present invention is defined by the appended claims.

Claims

1. An electrospinning film fixing device, characterized in that: include: A first fixing member (110) is configured to be disposed on a first side of the film, wherein the first fixing member (110) has at least two partitions (111); as well as The second fixing member (120) is configured to be disposed on the second side of the film, and the second fixing member (120) is clamped to the boundary portion of each partition (111) of the at least two partitions (111) so that the film forms a film unit in each partition (111).

2. The electrospinning film fixing device according to claim 1, characterized in that: One of the first fixing member (110) and the second fixing member (120) is provided with a slot, and the other of the first fixing member (110) and the second fixing member (120) is provided with a buckle, and the slot and the buckle cooperate to achieve the clamping of the first fixing member (110) and the second fixing member (120).

3. The electrospinning film fixing device according to claim 1, characterized in that: The second fixing member (120) comprises an annular member, and the annular member is arranged in a closed circumferential direction along the boundary of the partition (111).

4. The electrospinning film fixing device according to claim 1, characterized in that: The first fixing member (110) comprises a first annular member (112), the first annular member (112) being configured to form one partition (111) of the at least two partitions (111), and the second fixing member (120) comprises a second annular member (121), the second annular member (121) being configured to be disposed in the first annular member (112) and to be clamped and fixed to the first annular member (112).

5. The electrospinning film fixing device according to claim 4, characterized in that: The first annular member (112) is provided with a first clamping groove (113), and the second annular member (121) is provided with a first clamping buckle (122), and the first clamping buckle (122) is configured to be arranged in the first clamping groove (113).

6. The electrospinning film fixing device according to claim 1, characterized in that: The first fixing member (110) comprises a first plate (114) and at least two first annular members (112), wherein the at least two first annular members (112) are detachably arranged on the first plate (114) to form the at least two partitions (111).

7. The electrospinning film fixing device according to claim 6, characterized in that: The second fixing member (120) comprises at least two second annular members (121), and the at least two second annular members (121) are configured to be respectively arranged in the at least two first annular members (112) in a one-to-one correspondence, and the second annular members (121) are clamped with the first annular members (112).

8. The electrospinning film fixing device according to claim 7, characterized in that: The second fixing member (120) comprises a second plate (123), the at least two second annular members (121) are detachably arranged on the second plate (123), the second plate (123) is configured to carry the at least two second annular members (121) and to be respectively arranged one-to-one in the at least two first annular members (112) on the first plate (114), and the first plate (114) and the second plate (123) are configured to be disassembled after the first annular member (112) and the second annular member (121) are clamped.

9. The electrospinning film fixing device according to claim 6, characterized in that: The first annular member (112) is provided with a first clamping groove (113), the first plate (114) is provided with a first groove (115) for accommodating the first annular member (112), the side wall of the first groove (115) is provided with a second clamping buckle (1151), and the second clamping buckle (1151) is configured to be arranged in the first clamping groove (113).

10. The electrospinning film fixing device according to claim 8, characterized in that: The second annular member (121) is provided with a first protrusion (125), the second plate (123) is provided with a second groove (126) for accommodating the second annular member (121), a through hole (127) is provided in the second groove (126), and the first protrusion (125) is configured to be inserted into the through hole (127).

11. The electrospinning film fixing device according to claim 10, characterized in that: The invention also includes a third plate (130), wherein the third plate (130) is provided with a second protrusion (131), and the third plate (130) is configured to be arranged on a side of the second plate (123) opposite to the second groove (126), and the second protrusion (131) is configured to be inserted into the through hole (127) and push the first protrusion (125).

12. The electrospinning film fixing device according to claim 1, characterized in that: The second fixing member (120) comprises two strip members (128), and the two strip members (128) are respectively arranged on two opposite sides of the boundary of the partition (111) in a one-to-one correspondence.

13. The electrospinning film fixing device according to claim 12, characterized in that: The first fixing member (110) comprises a strip partition (116), and second slots (117) are respectively provided on both sides of the strip partition (116). The two strip members (128) are respectively provided with third buckles (129), and the third buckles (129) are configured to be arranged in the second slots (117) so that the strip members (128) and the first fixing member (110) are clamped and fixed.

14. An electrospinning film stacking device, characterized in that: include: A first component (210) having a receiving groove on its top; A third annular member (230) having an outer diameter smaller than an inner circumferential dimension of the receiving groove; the third annular member (230) is configured to be disposed in the receiving groove; An electrospinning film fixture as claimed in any one of claims 1 to 13; the film unit formed by the fixture is configured to be placed on top of the first component (210), and the circumferential dimension of the film unit is larger than the circumferential dimension of the first component (210); A second component (220) having a circumferential dimension smaller than an inner circumferential dimension of the containing groove; the second component (220) is configured to abut against the film unit from the top of the film unit; a fifth annular member (250) having an inner diameter greater than the circumferential dimensions of the second member (220) and the first member (210); the fifth annular member (250) is configured to be sleeved on the second member (220) and to press against the film unit so that the outer edge of the film unit is circumferentially wrapped around the outer circumference of the first member (210); as well as The fourth ring-shaped member (240) has an outer diameter smaller than the inner diameter of the third ring-shaped member (230); the fourth ring-shaped member (240) is configured to be arranged on the inner side of the third ring-shaped member (230) and clamped with the third ring-shaped member (230) after the plurality of the film units are stacked in sequence and the outer edges of the plurality of the film units are circumferentially wrapped around the outer circumference of the first member (210) in sequence; the third ring-shaped member (230) is configured to cooperate with the fourth ring-shaped member (240) to fix the stacked plurality of film units.

15. An electrospinning film winding device, characterized in that: include: Strip plate (310); The electrospinning film fixing device according to any one of claims 1 to 13; the fixing device is configured to form a strip-shaped film unit, and the film unit is configured to be attached to the top surface of the strip plate (310); as well as The film cutting member (320) is configured to cut off the connection between the film unit and the fixing device along the length direction of the film unit, and the film unit is wound into a roll shape.

16. The electrospinning film winding device according to claim 15, characterized in that: The invention also includes a reel (330), wherein the reel (330) is configured to be disposed at an end of the film unit so that the film unit is wound around the reel (330).

17. The electrospinning film winding device according to claim 15, characterized in that: The film breaking component (320) comprises: A first fixing block (321); A second fixing block (322); and Two shearing pieces (323), each shearing piece (323) comprises a first blade piece (3231) and a second blade piece (3232), and the two first blade pieces (3231) are fixedly arranged between the first fixing block (321) and the second fixing block (322) at intervals; the two shearing pieces (323) are configured to simultaneously cut off the connection between the film unit and the fixing device along the length direction of the film unit.

18. The electrospinning film winding device according to claim 15, characterized in that: The film breaking component (320) comprises: A first main body member (324); a first side member (325); a second side member (326); and Two blades (327), one of which is fixed to the first side of the first main body (324) via the first side piece (325), and the other blade (327) is fixed to the second side of the first main body (324) via the second side piece (326); the two blades (327) are configured to simultaneously cut off the connection between the film unit and the fixing device along the length direction of the film unit.

19. An electrospinning film preparation system, characterized in that: include: Power supply (410); The wire collector (420) is configured to rotate, and the wire collector (420) comprises: At least two rods (421) connected to the negative pole of the power source (410) or to the ground; A first connecting member (422) connected to the first ends of the at least two rods (421); and A second connecting member (423) connected to the second ends of the at least two rods (421); an injection member (430) connected to the positive electrode of the power source (410); the injection member (430) is configured to inject a solution toward the collector (420) to form an electrospinning film on the at least two rods (421); and The electrospun film fixing device according to any one of claims 1 to 13, wherein the fixing device is configured to fix the electrospun film.

20. The electrospinning film preparation system according to claim 19, characterized in that: It also includes a guide rail (440), the injection member (430) is movably disposed on the guide rail (440), and the extension direction of the guide rail (440) is parallel to the extension direction of the at least two rod members (421).

21. The electrospinning film preparation system according to claim 19, characterized in that: The invention also includes a first test component (500), wherein the first test component (500) includes a first frame (510) and two clamping members (520); the first frame (510) includes a first frame bar (511) and a second frame bar (512) that are arranged opposite to each other, and a third frame bar (513) and a fourth frame bar (514) that are arranged opposite to each other; the first frame bar (511) and the second frame bar (512) are respectively provided with a first breakable portion (530); the two clamping members (520) are respectively provided on the third frame bar (513) and the fourth frame bar (514) in a one-to-one correspondence to clamp the electrospun film; the first breakable portion (530) is configured to be broken so as to perform a tensile test on the electrospun film.

22. The electrospinning film preparation system according to claim 19, characterized in that: The invention also comprises a second test assembly (600), wherein the second test assembly (600) comprises two second frames (610), wherein two opposite sides of the two second frames (610) are respectively provided with a second breakable portion (620), wherein the two second frames (610) are configured to clamp the electrospun film and immerse the electrospun film in nitrogen liquid, and the second breakable portions (620) of the two second frames (610) are configured to break so as to perform a brittle fracture test on the electrospun film.

23. The electrospinning film preparation system according to claim 19, characterized in that: The invention also comprises a third test assembly (700), wherein the third test assembly (700) comprises a plate (710) and a conductive adhesive (720), wherein a boss (711) is provided on the plate (710), and the conductive adhesive (720) is configured to press out a recess through the boss (711), and the conductive adhesive (720) is configured to carry an electrospun film so as to perform an electron microscope scanning test on the electrospun film.

24. The electrospinning film preparation system according to claim 19, characterized in that: The invention also includes a fourth test component (800), wherein the fourth test component (800) includes a sixth ring-shaped member (810) and a seventh ring-shaped member (820), wherein the outer diameter of the sixth ring-shaped member (810) is smaller than the inner diameter of the seventh ring-shaped member (820), the sixth ring-shaped member (810) is arranged on the first side of the electrospun film, and the seventh ring-shaped member (820) is configured to be arranged on the second side of the electrospun film, the sixth ring-shaped member (810) is configured to be arranged inside the seventh ring-shaped member (820) and protrude from the seventh ring-shaped member (820), the sixth ring-shaped member (810) is configured to clamp the electrospun film with the seventh ring-shaped member (820), and the portion of the sixth ring-shaped member (810) protruding from the seventh ring-shaped member (820) is covered with the electrospun film, so as to perform a hydrophilicity and hydrophobicity test on the electrospun film.

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