Sesame oil extraction process

Abstract

The invention relates to the field of edible oil extraction, and discloses a sesame oil extraction process, which comprises the following steps: raw material pretreatment: carrying out primary treatment on sesame through cleaning, drying and crushing; carrying out enzymatic assisted cell wall rupture, and carrying out composite degradation on the cell wall by utilizing cellulase, pectinase and protease; subcritical fluid extraction: extracting the sesame oil by using a solvent such as butane or propane under low-temperature and low-pressure conditions; oil refining: removing impurities and improving the oil quality through physical filtration, enzymatic degumming and vacuum deodorization; and packaging a finished product, namely filling the refined sesame oil into a light-proof sealed container. Compared with a traditional process, the method has the advantages that the oil extraction rate and the functional component retention rate are remarkably increased, the peroxide value is reduced, the extraction time is remarkably shortened, and the method is high in process adaptability, environmentally friendly, capable of saving energy and suitable for industrial popularization.

Description

Technical Field

[0001] The invention relates to the technical field of edible oil extraction, in particular to a sesame oil extraction process. Background Art

[0002] Sesame oil is an edible oil with high nutritional value, and has attracted much attention because it is rich in functional ingredients such as sesamol and sesamin. However, traditional sesame oil extraction processes, such as pressing and solvent extraction, have many problems, which limit the further improvement of sesame oil extraction efficiency and product quality.

[0003] In the traditional pressing process, although the mechanical equipment is relatively simple, the integrity of the cell wall is not effectively destroyed, resulting in incomplete oil release and low oil yield. In addition, due to the high temperature during the pressing process, functional ingredients such as sesamol and sesamin are easily degraded, seriously affecting the nutritional value of sesame oil. In the solvent extraction process, organic solvents such as n-hexane are usually used to dissolve the oil. Although this method can improve the extraction efficiency, the solvent requires a higher operating temperature, which not only aggravates the oxidation of the oil, but may also lead to the loss of functional ingredients. More importantly, the environmental protection and safety issues of solvent residues also need to be urgently addressed.

[0004] Among the existing technologies, some studies have attempted to introduce enzymatic-assisted technology to improve the efficiency of cell wall rupture, but often only a single enzyme is used. The limitations of enzymatic action make it difficult to achieve complete cell wall rupture, and the oil extraction rate and the release of functional ingredients have not been significantly improved. At the same time, the existing technology has less exploration of mild extraction conditions, and is still mainly based on high temperature and high pressure, which is insufficient for the protection of sensitive functional ingredients. Overall, the existing technology still lacks an innovative process that can efficiently extract oils and effectively retain functional ingredients while taking into account environmental protection, safety and economy. Summary of the invention

[0005] In view of the shortcomings of the prior art, the present invention provides a sesame oil extraction process, which can efficiently extract sesame oil under low temperature and environmentally friendly conditions, while retaining the functional ingredients to the greatest extent and improving the quality of the oil.

[0006] To achieve the above objectives, the present invention is implemented by the following technical scheme: a sesame oil extraction process, comprising the following steps:

[0007] Raw material pretreatment: washing, drying and crushing sesame seeds;

[0008] Enzymatically assisted cell wall rupture: The broken sesame seeds are mixed with enzyme solution, and the sesame cell walls are enzymatically degraded using cellulase, pectinase and protease;

[0009] Subcritical Fluid Extraction: Extraction of sesame oil under subcritical fluid conditions;

[0010] Oil refining: The extracted sesame oil is subjected to physical filtration, enzymatic degumming and vacuum deodorization;

[0011] Finished product packaging: Filling the refined sesame oil.

[0012] Preferably, the washing step in the raw material pretreatment comprises mixing sesame with water at a water to sesame mass ratio of 1:10-1:15, the water temperature is 25-35° C., and the washing time is 10-15 minutes.

[0013] Preferably, in the enzymatically assisted cell wall disruption step, the enzyme solution comprises:

[0014] Cellulase, concentration 0.4%-0.6% (w / v);

[0015] Pectinase, concentration 0.2%-0.4% (w / v);

[0016] Protease, concentration 0.1%-0.3% (w / v);

[0017] The mass ratio of enzyme solution to sesame is 1:2-1:3, the temperature of enzymatic reaction is 45-55°C, and the time is 60-90 minutes.

[0018] Preferably, the enzymatically assisted cell wall disruption step uses citric acid-sodium citrate buffer as a solvent, and the pH value of the buffer is 5.0-5.5.

[0019] Preferably, in the subcritical fluid extraction step, subcritical butane is used as the solvent, the mass ratio of butane to sesame is 4:1-6:1, the extraction temperature is 35-45° C., the extraction pressure is 0.3-0.6 MPa, and the extraction time is 15-25 minutes.

[0020] Preferably, after the subcritical fluid extraction step, butane is recovered by vacuum distillation equipment, with a recovery rate of not less than 95%, a distillation temperature of 40-50° C., and a vacuum degree of 0.05-0.1 MPa.

[0021] Preferably, the oil refining step comprises:

[0022] Physical filtration through a filter membrane with a pore size of 0.2-0.5 μm;

[0023] Adding phospholipase for enzymatic degumming, the concentration of phospholipase is 0.1%-0.2% (w / v), the reaction temperature is 25-30°C, and the reaction time is 30-60 minutes;

[0024] The sesame oil is deodorized by a low-temperature vacuum deodorization device, the deodorization temperature is 50-60°C, and the vacuum degree is 0.05-0.1MPa.

[0025] Preferably, in the enzymatic degumming step, sesame oil and deionized water are mixed in a mass ratio of 1:10-1:15, and separated by a centrifuge to obtain refined oil.

[0026] Preferably, in the vacuum deodorization step, low-temperature steam is used as the deodorization medium, the steam flow rate is 1-2 L / min, and the deodorization time is 20-30 minutes.

[0027] Preferably, the finished product packaging step uses a light-proof food-grade container, the filling temperature is 20-25° C., and the container storage conditions are a temperature of 15-25° C. and a humidity of less than 60%.

[0028] The present invention provides a sesame oil extraction process. It has the following beneficial effects:

[0029] 1. The present invention significantly improves the extraction rate of sesame oil by combining enzymatic assisted cell wall rupture with subcritical fluid extraction. In the composite enzymatic treatment process, multiple enzymes work synergistically to effectively destroy the cell wall and release more bound oil; at the same time, subcritical fluid solvents (such as butane and propane) have high solubility and quickly dissolve oil under low temperature and low pressure conditions, reducing the amount of oil residue.

[0030] 2. The present invention adopts enzymatic treatment and subcritical extraction technology of low temperature operation to effectively protect the functional ingredients (such as sesamol and sesamin) in sesame oil. These ingredients are easily degraded under high temperature or strong acid and alkali conditions, and the present invention reduces the loss of ingredients to the greatest extent by optimizing temperature and extracting solvent, thereby ensuring the nutritional value and antioxidant properties of sesame oil.

[0031] 3. The present invention completes oil extraction under low-temperature, closed subcritical conditions, avoiding the risk of oxidation caused by high-temperature extraction. At the same time, selective solvents are used to isolate oxygen contact, significantly reducing the peroxide value (POV) of the oil.

[0032] 4. The present invention is suitable for efficient processing of different sesame raw materials through flexible process parameter settings (such as enzyme concentration, solvent type and extraction conditions), and is highly compatible with modern industrial equipment. Its low temperature operation, high solvent recovery rate (>95%) and environmentally friendly design make the process of the present invention stable in large-scale production and provide reliable technical support for the development of high-quality sesame oil products. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 It is a schematic diagram of the process flow of the present invention. DETAILED DESCRIPTION

[0034] The following will be combined with the drawings in the specification of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0035] Please refer to the attached Figure 1 The present invention provides a sesame oil extraction process, comprising the following steps:

[0036] S1. Raw material pretreatment

[0037] The raw material is pretreated in step S1 to ensure that the oil and functional components in the sesame can be effectively released and remain intact in the subsequent extraction step. Specifically, the raw material pretreatment step includes three sub-steps: washing, drying and crushing. Each step is closely related to the subsequent enzymatic assisted cell wall rupture step and is an important prerequisite for ensuring the efficiency of enzymatic action and subcritical fluid extraction.

[0038] Generally speaking, the main purpose of raw material pretreatment is to remove impurities in sesame raw materials, reduce moisture content, improve cell wall permeability and increase specific surface area, thereby providing favorable conditions for subsequent enzymatic reactions and solvent extraction.

[0039] In some embodiments, the cleaning in the pretreatment step mainly removes mud, dust and some water-soluble impurities on the surface of sesame seeds by deionized water or weak alkaline solution; the drying process reduces the moisture content of sesame seeds by low temperature conditions, while trying to avoid the damage of functional ingredients to high temperature; and the crushing process moderately crushes the sesame seeds by mechanical equipment to increase the physical contact area of ​​sesame seeds and release some soluble components.

[0040] In this embodiment, the washing step first places the sesame raw material in a washing tank or a drum-type washing device, and uses deionized water as the main washing medium. In order to further enhance the washing effect, a sodium bicarbonate solution (NaHCO3) with a concentration of 0.01-0.03 mol / L can be added to soften the polysaccharide and protein structure on the surface of the sesame by a weak alkaline solution to remove attached impurities.

[0041] Specifically, the mass ratio of water to sesame is set to 1:10-1:15, the temperature of the cleaning liquid is controlled within the range of 25°C to 35°C, and the cleaning time is 10 to 15 minutes. As a possible implementation method, the cleaning equipment can adopt a circulating water system to save water resources, and can be equipped with a vibration device to improve cleaning efficiency.

[0042] In some embodiments, in order to avoid the influence of residual alkali solution on subsequent processes, the washed sesame seeds can be rinsed with deionized water for multiple times (usually 2 to 3 times) until the pH of the rinse solution is close to neutral.

[0043] In this embodiment, the washed sesame seeds need to be dried. The drying is mainly carried out using circulating air drying equipment or heat pump drying equipment at a low temperature of 50° C. to 60° C. In order to avoid uneven drying, the sesame seeds are turned over every 30 minutes during the drying process to ensure that the sesame seeds are evenly heated.

[0044] The drying time is generally 3 to 5 hours, and the specific time can be adjusted according to the equipment performance and the moisture content of sesame. As an option, intelligent control equipment with humidity monitoring function can be used to ensure that the final moisture content of sesame is controlled within the range of 8% to 10% to meet the needs of subsequent processes.

[0045] In some embodiments, the circulating wind speed of the drying equipment can be set to 1.5 to 2.5 m / s, and this parameter range can be adjusted through experiments to balance the drying efficiency and sesame quality. In a possible implementation, drying under low temperature conditions can effectively reduce the oxidation risk of sesame oil in the pretreatment stage.

[0046] In this embodiment, the dried sesame seeds are crushed by a roller crusher. Generally, the roller gap of the crusher can be adjusted to 0.5 to 1.0 mm to ensure that the sesame seeds are crushed into appropriate particles, thereby increasing the specific surface area without causing excessive crushing.

[0047] Specifically, the rotation speed of the crusher is controlled at 80 to 120 rpm during the crushing process to reduce the damage of mechanical shearing to the sesame oil. In some embodiments, in order to reduce dust pollution and electrostatic adhesion of sesame particles, a negative pressure exhaust device can be installed on the crushing equipment, and an anti-sticking roller material can be used to reduce the adhesion of the crushed objects.

[0048] As an option, a multi-stage crushing process can be used. In the first stage of crushing, the sesame seeds are crushed into coarse particles with a particle size of about 1.0 mm; in the second stage of crushing, the coarse particles are further crushed into fine particles with a particle size range of 0.5 to 0.8 mm. Multi-stage crushing not only improves the uniformity of sesame particles, but also avoids the problem of one-time crushing resulting in too fine or too coarse particles.

[0049] It should be noted that the specific parameters of the pretreatment step in the present invention can be appropriately adjusted according to the properties of different sesame raw materials. For example, for sesame with more impurities, the washing time can be extended or the number of washing times can be increased; for sesame with higher humidity, the drying temperature can be appropriately increased or the drying time can be extended; for sesame with harder texture, the roller gap of the crusher can be appropriately reduced to achieve the best crushing effect.

[0050] Through the above-mentioned cleaning, drying and crushing processing steps, the sesame raw material is fully pre-treated, which not only removes impurities and excess moisture, but also optimizes the physical structure of the sesame cell wall.

[0051] S2, Enzyme-assisted cell wall disruption

[0052] Step S2 is to rupture the sesame cell wall by enzymatic assistance to create favorable conditions for subsequent subcritical fluid extraction. Enzymatic treatment is one of the key steps of the process, and its purpose is to use a specific enzyme system (including cellulase, pectinase and protease) to selectively degrade the cellulose, pectin and protein matrix in the sesame cell wall, thereby destroying the integrity of the sesame cell wall and releasing the oil and functional components (such as sesamol and sesamin) in the cell.

[0053] In general, in order to achieve the best cell wall degradation effect, it is necessary to adjust the enzyme solution ratio and reaction conditions according to the physical and chemical properties of sesame and the particle size after pretreatment, such as key parameters such as pH, temperature, solid-liquid ratio and reaction time of the enzyme solution. Specifically, the implementation of step S2 includes both the preparation process of the enzyme solution and the operation steps of the enzymatic reaction, and the operation method and optimization of the process conditions have been fully verified.

[0054] In some embodiments, the enzymatic treatment combines the destructive effect of cellulase on the fiber structure of the cell wall, the decomposition of intercellular adhesives by pectinase, and the degradation of matrix proteins by proteases, so that the degradation of sesame cell walls is more thorough.

[0055] In this embodiment, the enzyme solution is prepared using three enzyme preparations, cellulase, pectinase and protease, to achieve multi-level degradation of sesame cell walls. The role of cellulase is to decompose the cellulose backbone of the cell wall, and the concentration is set to 0.4%-0.6% (w / v). Pectinase is mainly used to degrade pectin, with a concentration range of 0.2%-0.4% (w / v), thereby loosening the cell wall structure. Protease is used to decompose the protein matrix combined with oil in the cell wall, with a concentration range of 0.1%-0.3% (w / v).

[0056] As an option, the solvent of the enzyme solution is 0.1 mol / L citric acid-sodium citrate buffer, which is used to adjust the pH value of the enzyme solution to 5.0-5.5. The buffer not only provides a suitable active environment for the enzyme, but also avoids the loss of enzyme activity due to pH fluctuations.

[0057] In a possible implementation, the enzyme solution is continuously stirred by a magnetic stirrer during preparation, and the stirring time is controlled within 5-10 minutes to ensure that the three enzymes are fully dissolved and evenly distributed in the solution.

[0058] In this embodiment, the enzymatic reaction mixes the crushed sesame particles with the enzyme solution at a solid-liquid ratio of 1:2-1:3 (w / v), and then transfers them to a temperature-controlled reactor for reaction. The reaction temperature is set at 45°C to 55°C, the reaction time is 60 to 90 minutes, and the stirring rate is 50 to 100 rpm. The main purpose of stirring is to ensure sufficient contact between the enzyme solution and the sesame particles, thereby improving the reaction efficiency.

[0059] Generally, in order to avoid the decrease of enzyme activity or oil oxidation due to the increase of reaction temperature, the reactor can be equipped with a water bath temperature control system. In some embodiments, the temperature rise gradient of the enzymatic reaction is controlled by heating in stages, for example, maintaining 45°C for the first 30 minutes, then gradually increasing to 55°C, and finally maintaining a constant temperature to complete the reaction.

[0060] Specifically, during the reaction process, samples can be taken every 10 minutes to detect the degree of degradation of the sesame cell wall, and the detection methods include microscopic observation or turbidimetric determination. The test results show that when the degradation rate of cellulose and pectin reaches 70%-90%, the enzymatic reaction is basically completed.

[0061] In a possible implementation, after the enzymatic reaction is completed, the mixture is separated into solid and liquid by a centrifuge. The speed of the centrifuge is set to 3000 to 5000 rpm for 5 to 10 minutes to ensure maximum recovery of the liquid product containing sesame oil and functional ingredients. In some embodiments, the separated solid residue can be subjected to a secondary enzymatic treatment to improve the overall extraction rate.

[0062] As an option, in order to avoid interference of residual enzymes with subsequent extraction processes, hot water at 60°C may be added to the reactor after the enzymatic reaction to quickly inactivate the enzymes in the reaction system for 2 to 5 minutes.

[0063] This embodiment also discloses an extended operation scheme, which introduces a combination of mechanical stirring and ultrasonic assistance during the enzymatic reaction process. Specifically, an ultrasonic transducer is set in the reactor, the ultrasonic frequency is 20-40kHz, and the action time is 1 minute of ultrasonic treatment every 10 minutes. The mechanical action of ultrasound can further enhance the penetration effect of the enzyme solution on the cell wall, and at the same time promote the release of oil and functional ingredients.

[0064] The enzymatic-assisted cell wall rupture treatment step of the present invention not only significantly improves the degradation efficiency of the sesame cell wall, but also lays a good foundation for subsequent subcritical fluid extraction.

[0065] S3, Subcritical Fluid Extraction

[0066] Step S3 is mainly for the sesame material after enzymatic treatment, and the sesame oil is efficiently extracted by the solvent extraction technology under subcritical conditions. This step utilizes the excellent solubility of subcritical fluid solvents under relatively low temperature and pressure, which can not only efficiently extract oil, but also effectively retain the functional ingredients (such as sesamol and sesamin) in sesame. By accurately controlling the extraction conditions (including solvent selection, temperature, pressure and extraction time), a high extraction rate and the integrity of the functional ingredients are achieved.

[0067] In general, subcritical fluid extraction has the characteristics of high dissolution selectivity, mildness, environmental protection, and safe operation, and is particularly suitable for the extraction of sesame oil with high sensitive components. Specifically, the S3 step includes three key links: solvent selection and pretreatment, extraction process, and solvent recovery. The setting and execution of its process parameters are directly related to the extraction efficiency and product quality.

[0068] In some embodiments, subcritical butane is selected as the solvent in this step, based on its low boiling point (-0.5°C) at normal pressure and good solubility for sesame oil. At the same time, butane has high operational safety and can be efficiently recovered by vacuum distillation technology after extraction.

[0069] In this embodiment, the solvent selected for subcritical fluid extraction is butane with a purity of not less than 99.5%. To ensure the stability of the extraction solvent, butane needs to be degassed before use. The specific method is to place the solvent in a vacuum environment and stir it for 10 to 15 minutes. The vacuum degree is 0.05 to 0.1 MPa to remove dissolved oxygen and other trace gases in the solvent.

[0070] As a possible implementation method, butane and sesame material are mixed in a mass ratio of 4:1 to 6:1, and the specific ratio can be adjusted according to the oil content of the material after enzymatic treatment. The mixed extraction system is transferred to a subcritical fluid extraction device, sealed and pressurized to within the operating pressure range.

[0071] In this embodiment, the subcritical fluid extraction equipment is precisely controlled by a temperature and pressure control device. The extraction temperature is set to 35°C to 45°C, and the extraction pressure range is 0.3MPa to 0.6MPa. As an option, the extraction time can be adjusted to 15 to 25 minutes according to the extraction rate and solubility of the target oil.

[0072] Specifically, during the extraction process, in order to ensure that sesame oil and butane are in full contact, the continuous flow of the solvent can be achieved through a stirring mechanism or a circulating pump in the equipment. The stirring rate is recommended to be controlled at 50 to 100 rpm, and the circulation frequency is once every 5 minutes. In some embodiments, a liquid level monitoring system can also be set to control the ratio of material to solvent in real time to prevent insufficient or excessive solvent during the extraction process.

[0073] In a possible implementation, after the extraction is completed, the mixed liquid is initially separated into solid and liquid by a separation device, and the extracted oil-solvent mixed liquid enters a vacuum distillation device. The main purpose of vacuum distillation is to recover the solvent butane while avoiding the influence of high temperature evaporation on the functional components.

[0074] In this embodiment, the vacuum degree of the distillation equipment is controlled at 0.05 to 0.1 MPa, and the distillation temperature ranges from 40° C. to 50° C. The distillation time is generally 20 to 30 minutes, which can be adjusted according to the solvent recovery rate. The solvent butane can be recycled after distillation and condensation, and the recovery rate is not less than 95%.

[0075] As an option, in order to reduce the impact of residual solvents on the quality of the oil, the oil can be quickly cooled after the distillation to reduce the temperature to room temperature (20°C to 25°C).

[0076] In some embodiments, in order to further improve the extraction efficiency and the retention rate of functional components, an appropriate amount of co-solvent (such as ethanol) can be added to the extraction system, and the addition ratio is 5% to 10% of the mass of butane. The main function of the co-solvent is to improve the solvent system's solubility for polar components (such as sesamol) while reducing the amount of solvent used.

[0077] In another possible implementation, the extraction equipment can be equipped with an online monitoring function to detect the oil concentration in the extract in real time by ultraviolet spectroscopy or turbidimetry. When the extraction efficiency drops to a set threshold (for example, the oil content in the extract is less than 0.1%), the system can automatically terminate the extraction process.

[0078] Through the above operations, the subcritical fluid extraction step of this embodiment can achieve efficient extraction of sesame oil under mild conditions and retain the functional components therein to the maximum extent.

[0079] S4. Oil refining

[0080] Step S4 is to further purify and treat the crude oil obtained by subcritical fluid extraction, aiming to remove impurities, colloids and peculiar smells therein, and improve the purity and quality of sesame oil. This step combines multiple processes such as physical filtration, enzymatic degumming and vacuum deodorization to achieve efficient refining and quality stability of sesame oil. By strictly controlling the parameters of each process, the integrity of the functional components in sesame oil is guaranteed, while reducing the content of oxidation products and volatile impurities.

[0081] Generally speaking, the key to the oil refining step is to maintain a low-temperature operating environment to avoid oil oxidation or decomposition of functional components caused by high-temperature treatment. Specifically, the S4 step includes three sub-steps: physical filtration, enzymatic degumming and vacuum deodorization, and all processes have been carefully designed and verified.

[0082] In some embodiments, filtration mainly uses high-precision filter membranes to remove suspended impurities; degumming removes colloids and impurity proteins through phospholipase degradation; and deodorization removes solvent residues and odors through vacuum distillation technology. These treatment methods cooperate with each other to form a complete refining process.

[0083] In this embodiment, physical filtration uses a precision filter membrane to perform preliminary filtration on the crude oil. Specifically, the pore size of the filter membrane is set to 0.2 μm to 0.5 μm to ensure the removal of fine particles and suspended matter in the oil. The filtration equipment uses a positive pressure filtration system, the pressure is controlled at 0.1 MPa to 0.2 MPa, and the filtration temperature is maintained in the range of 20°C to 30°C.

[0084] As a possible implementation method, the filtration process can be set with an automatic impurity removal function to backwash the filter membrane every 10 minutes to prevent the accumulation of impurities from affecting the filtration efficiency. In some embodiments, to further improve the filtration effect, an appropriate amount of filter aid (such as diatomaceous earth) can be added before filtration, and the amount of filter aid added is 0.1% to 0.5% of the weight of the oil.

[0085] In this embodiment, enzymatic degumming is to remove phospholipids and impurity proteins in oil by adding phospholipase. Specifically, the enzyme solution is prepared according to the ratio of degumming treatment liquid to crude oil of 1:10 to 1:15 (w / v), the concentration of phospholipase is 0.1% to 0.2% (w / v), and the reaction temperature is controlled at 25°C to 30°C.

[0086] In a possible implementation, the pH value of the enzyme solution is adjusted to 6.5 to 7.0 to provide the most suitable enzymatic reaction conditions. In order to enhance the contact between the enzyme solution and the oil, a stirring device is used to maintain the stirring rate at 50 rpm to 80 rpm, and the reaction time is 30 minutes to 60 minutes. After the reaction is completed, the water phase and the oil phase are separated by a centrifuge, and the centrifugal speed is set to 3000 rpm to 5000 rpm for 5 to 10 minutes.

[0087] In some embodiments, in order to further reduce the impurity content in the oil, an appropriate amount of weak acid (such as citric acid) can be added after enzymatic degumming, and the acid concentration is 0.01 mol / L to 0.05 mol / L. By adjusting the pH value of the oil to 4.0 to 5.5, it helps to further remove impurities.

[0088] In this embodiment, vacuum deodorization uses low-temperature distillation technology to remove solvent residues and odor substances in oil. Specifically, the degummed oil is transferred to a vacuum deodorization device, the vacuum degree is controlled at 0.05MPa to 0.1MPa, and the deodorization temperature is set at 50°C to 60°C.

[0089] As an option, low-temperature steam can be used as the deodorization medium, with a steam flow rate of 1L / min to 2L / min and a deodorization time of 20 minutes to 30 minutes. In a possible implementation, the deodorization equipment can be equipped with an online odor monitoring system to adjust the deodorization time by real-time detection of odor intensity to ensure that the odor index of the final oil meets the quality requirements.

[0090] In some embodiments, in order to avoid the loss of functional components during the deodorization process, the oil can be quickly cooled after deodorization, and the cooling temperature is controlled in the range of 20° C. to 25° C. At the same time, the oxidation of the oil can be further delayed by adding 0.01% to 0.02% of a natural antioxidant (such as vitamin E).

[0091] Through the physical filtration, enzymatic degumming and vacuum deodorization treatment steps of this embodiment, impurities and odors in the crude oil are effectively removed, and the clarity and quality of the oil are significantly improved.

[0092] S5. Finished product packaging

[0093] Step S5 is to fill and seal the refined sesame oil to ensure that the product maintains its quality and stability of functional ingredients during storage and transportation. Through scientific and reasonable packaging design, sesame oil can be effectively protected from environmental factors such as oxidation, light and humidity. At the same time, this step combines technical means such as aseptic filling and light-proof packaging to further extend the shelf life of the product and meet the market demand for high-quality sesame oil.

[0094] Generally speaking, finished product packaging needs to take into account food-grade safety, the protective performance of packaging materials, and the operability of packaging technology. Specifically, the S5 step includes three main links: container selection, aseptic filling, and sealed storage. The process parameters of each link are optimized to ensure that sesame oil can maintain its original flavor and nutritional content after packaging.

[0095] In some embodiments, the filling process also needs to be precisely controlled in conjunction with automated equipment to ensure consistency in product volume and the cleanliness of the filling environment.

[0096] In this embodiment, the container used for the finished product packaging is a light-proof glass bottle or an aluminum foil bag, the inner wall of which is treated with a food-grade coating to prevent chemical reactions between grease and the container. As an option, a multi-layer composite plastic bottle with low oxygen permeability and high mechanical strength can be used, wherein the inner layer is made of polyethylene or polypropylene and the outer layer is coated with an anti-ultraviolet coating.

[0097] In a possible implementation, the packaging container needs to be sterilized by steam or ultraviolet light before use, with the sterilization temperature controlled at 80° C. to 100° C. and the sterilization time being 15 minutes to 30 minutes to ensure the sterility of the container.

[0098] In this embodiment, the refined sesame oil is filled in an aseptic filling device. The filling temperature is controlled at 20°C to 25°C to avoid oil oxidation or loss of functional components due to high temperature filling. The filling device is equipped with an automatic liquid level detection device, which can accurately control the filling amount of each packaging unit with an error of no more than ±0.1%.

[0099] As an option, nitrogen filling technology can be used during the filling process to replace the oxygen in the package with high-purity nitrogen (purity ≥ 99.9%) to reduce the risk of contact between oil and oxygen. The nitrogen filling pressure is controlled within the range of 0.02MPa to 0.05MPa.

[0100] In this embodiment, the sealing adopts automatic capping or heat sealing equipment, and the corresponding sealing method is selected according to the type of packaging container. Specifically, the glass bottle is sealed with a screw-type aluminum cap, and the sealing torque is 15 to 25 N·cm; the aluminum foil bag uses heat sealing technology, the heat sealing temperature is controlled at 120℃ to 140℃, and the sealing width is 5mm to 10mm.

[0101] In some embodiments, in order to further improve the sealing effect, the packaging container may be subjected to a secondary inspection after the sealing is completed. The inspection method includes a positive pressure test or a water immersion bubble method to ensure that the sealing of the container meets the requirements of transportation and storage.

[0102] In this embodiment, the sealed sesame oil needs to be stored in an environment with a temperature of 15° C. to 25° C. and a relative humidity of less than 60% to avoid oil deterioration caused by high temperature or high humidity. As a possible implementation method, a light shielding device can be installed in the storage area to prevent direct light from affecting the quality of the sesame oil.

[0103] In some embodiments, in order to enhance the market competitiveness of the product, functional labels such as anti-counterfeiting labels, QR code traceability systems, etc. may be added to the outside of the packaging container to meet consumers' demands for product quality and traceability information.

[0104] Through the above-mentioned container selection, aseptic filling and sealed storage operations, this embodiment can effectively ensure the quality stability of sesame oil after packaging, extend the shelf life of the product, and meet consumers' demand for high-quality sesame oil products.

[0105] In order to better understand the present invention, the above process is described in detail below in conjunction with specific embodiments.

[0106] Example 1: Sesame oil extraction process based on subcritical butane solvent

[0107] Step 1: Raw material pretreatment

[0108] Washing: Mix sesame seeds with deionized water at 25°C at a ratio of 1:10 (w / v) for 10 minutes; then rinse twice to remove residual impurities.

[0109] Drying: Treat in a circulating air dryer at 50℃ for 3 hours, and the moisture content drops to 8%.

[0110] Crushing: Use a roller crusher to crush the sesame seeds to a particle size of 0.8 mm.

[0111] Step 2: Enzymatically Assisted Cell Wall Disruption

[0112] Enzyme solution preparation: 0.5% (w / v) cellulase, 0.3% (w / v) pectinase, and 0.2% (w / v) protease were dissolved in a citric acid buffer solution at pH 5.0.

[0113] Reaction conditions: solid-liquid ratio 1:2 (w / v), temperature 45°C, reaction time 60 minutes, stirring rate 60 rpm.

[0114] Step 3: Subcritical Fluid Extraction

[0115] Extraction solvent: butane and sesame mixed ratio 4:1 (w / v), extraction temperature 35°C, extraction pressure 0.3MPa, extraction time 15 minutes.

[0116] Step 4: Oil Refining

[0117] Filtration: 0.5 μm pore size filter membrane, filtration temperature 30°C.

[0118] Enzymatic degumming: phospholipase concentration 0.15% (w / v), temperature 25°C, reaction time 30 minutes.

[0119] Deodorization: vacuum degree 0.05MPa, deodorization temperature 50℃, time 20 minutes.

[0120] Step 5: Finished product packaging

[0121] The aseptic filling temperature is 20℃, the product is stored in sealed glass bottles, and the storage environment temperature is 15℃.

[0122] Example 2: Sesame oil extraction process based on subcritical propane solvent

[0123] Step 1: Raw material pretreatment

[0124] Cleaning: Clean in 30°C sodium bicarbonate solution (0.02 mol / L) for 15 minutes, then rinse three times with deionized water.

[0125] Drying: Drying temperature is 60℃, time is 4 hours, moisture content drops to 9%.

[0126] Crushing: The crushing particle size is 1.0mm.

[0127] Step 2: Enzymatically Assisted Cell Wall Disruption

[0128] Enzyme solution preparation: 0.4% (w / v) cellulase and 0.2% (w / v) pectinase were dissolved in a buffer solution of pH 5.5.

[0129] Reaction conditions: solid-liquid ratio 1:3 (w / v), temperature 50°C, time 90 minutes, stirring rate 50 rpm.

[0130] Step 3: Subcritical Fluid Extraction

[0131] Extraction solvent: propane and sesame mixed ratio 5:1 (w / v), extraction temperature 40°C, extraction pressure 0.5MPa, extraction time 20 minutes.

[0132] Step 4: Oil Refining

[0133] Filtration: 0.2μm pore size filter membrane, filtration temperature 25℃.

[0134] Enzymatic degumming: phospholipase concentration 0.2% (w / v), pH 6.5, temperature 30°C, time 60 minutes.

[0135] Deodorization: vacuum degree 0.1MPa, deodorization temperature 60℃, time 30 minutes.

[0136] Step 5: Finished product packaging

[0137] Aseptic filling temperature is 25℃, aluminum foil bag is heat sealed, and storage environment humidity is less than 60%.

[0138] Example 3: Cold pressing and subcritical fluid combined process

[0139] Step 1: Raw material pretreatment

[0140] Cleaning: Soak in 25℃ deionized water for 10 minutes.

[0141] Drying: temperature 50℃, time 3 hours, moisture content drops to 10%.

[0142] Crushing: Particle size is 0.6mm.

[0143] Step 2: Enzymatically Assisted Cell Wall Disruption

[0144] Enzyme solution preparation: cellulase 0.6% (w / v), protease 0.3% (w / v), buffer pH 5.2.

[0145] Reaction conditions: solid-liquid ratio 1:2 (w / v), temperature 45°C, time 60 minutes.

[0146] Step 3: Cold Pressing and Subcritical Fluid Extraction

[0147] Cold pressing: initial pressing pressure 50MPa, temperature 25℃, pressing time 10 minutes.

[0148] Subcritical fluid extraction: using butane, mixing ratio 3:1 (w / v), extraction temperature 35°C, pressure 0.4MPa.

[0149] Step 4: Oil Refining

[0150] Enzymatic degumming: phospholipase concentration 0.1% (w / v), temperature 25°C, time 30 minutes.

[0151] Deodorization: vacuum degree 0.08MPa, temperature 55℃.

[0152] Step 5: Finished product packaging

[0153] Aseptic filling temperature is 20℃, using multi-layer composite plastic bottles.

[0154] Embodiment 4: Processing technology of high-humidity sesame raw material

[0155] Step 1: Raw material pretreatment

[0156] Cleaning: 30℃ deionized water, cleaning time 15 minutes, rinse 3 times.

[0157] Drying: temperature 55℃, time 5 hours, moisture content dropped to 7%.

[0158] Crushing: Particle size is controlled at 1.0mm.

[0159] Step 2: Enzymatically Assisted Cell Wall Disruption

[0160] Enzyme solution preparation: cellulase 0.5% (w / v), pectinase 0.4% (w / v), protease 0.1% (w / v), buffer pH 5.0.

[0161] Reaction conditions: solid-liquid ratio 1:3 (w / v), temperature 50°C, time 90 minutes.

[0162] Step 3: Subcritical Fluid Extraction

[0163] Solvent: butane and sesame mixed ratio 6:1 (w / v), temperature 45°C, pressure 0.6MPa, extraction time 25 minutes.

[0164] Step 4: Oil Refining

[0165] Filtration: 0.3 μm pore size filter membrane, filtration temperature 30°C.

[0166] Deodorization: vacuum degree 0.07MPa, deodorization temperature 50℃, time 25 minutes.

[0167] Step 5: Finished product packaging

[0168] Aseptic filling, storage in glass bottles, sealed after nitrogen filling.

[0169] Example 5: Low temperature storage environment optimization process

[0170] Step 1: Raw material pretreatment

[0171] Cleaning: 25℃ deionized water, cleaning time 10 minutes.

[0172] Drying: temperature 50℃, time 4 hours, moisture content dropped to 9%.

[0173] Crushing: Particle size is 0.8mm.

[0174] Step 2: Enzymatically Assisted Cell Wall Disruption

[0175] Enzyme solution preparation: cellulase 0.4% (w / v), pectinase 0.3% (w / v), protease 0.2% (w / v), pH 5.5 buffer.

[0176] Reaction conditions: solid-liquid ratio 1:2.5 (w / v), temperature 45°C, time 60 minutes.

[0177] Step 3: Subcritical Fluid Extraction

[0178] The butane solvent mixing ratio was 5:1 (w / v), the extraction temperature was 40°C, the pressure was 0.5 MPa, and the time was 20 minutes.

[0179] Step 4: Oil Refining

[0180] Degumming: phospholipase concentration 0.1% (w / v), reaction time 30 minutes, temperature 25°C.

[0181] Deodorization: vacuum degree 0.05MPa, deodorization temperature 55℃, time 20 minutes.

[0182] Step 5: Finished product packaging

[0183] Storage conditions: temperature 15°C, humidity 50%, storage environment away from light.

[0184] Comparative experiment 1:

[0185] Objective: To verify the advantages of the composite enzymatic treatment in improving the sesame cell wall degradation efficiency, oil extraction rate and functional component release by comparing the effects of the composite enzymatic treatment (experimental group, Example 1) of the present invention with those of the single enzyme treatment (Comparative Example 1).

[0186] Experimental setup

[0187] Experimental group: The enzymatic-assisted cell wall disruption process (composite enzyme method) of Example 1 was used.

[0188] Cellulase: 0.5% (w / v).

[0189] Pectinase: 0.3% (w / v).

[0190] Protease: 0.2% (w / v).

[0191] Reaction conditions: temperature 45°C, time 60 minutes, solid-liquid ratio 1:2.

[0192] Comparative Example 1: Single enzyme treatment, using only cellulase at a concentration of 0.5% (w / v), and the other reaction conditions were consistent with the experimental group.

[0193] Experimental steps:

[0194] 1. Sample Preparation

[0195] 20 g of crushed sesame seeds were divided into groups and enzyme solution was prepared with buffer solution (pH 5.0).

[0196] Prepare enzyme solution according to the process requirements of the experimental group and the comparative example, add it to the corresponding sample, and mix well.

[0197] 2. Enzymatic reaction

[0198] The sample was placed in a constant temperature water bath oscillator, and the reaction temperature was controlled at 45°C, the reaction time was 60 minutes, and the oscillation speed was 60 rpm.

[0199] Samples were taken every 10 minutes and the cell wall degradation was observed under a microscope.

[0200] 3. Centrifugal separation

[0201] After the reaction, the sample was centrifuged (4000 rpm, 10 minutes) for solid-liquid separation, and the liquid phase was retained for determination.

[0202] 4. Determination of indicators

[0203] Cell wall degradation rate (%): The cell wall structure rupture was analyzed by image analysis using a microscope.

[0204] Oil extraction rate (%): the ratio of the mass of liquid oil to the total mass of the sample.

[0205] Functional ingredient content (mg / g): The contents of sesamol and sesamin were determined by high performance liquid chromatography (HPLC).

[0206] The experimental results are shown in the following table:

[0207] Experimental indicators Experimental group (complex enzyme method) Comparative Example 1 (single enzyme method) Cell wall degradation rate (%) 82.1 64.8 Oil extraction rate (%) 87.2 74.3 Sesamol content (mg / g) 3.41 2.68 Sesamin content (mg / g) 5.12 4.03

[0208] Experimental data show that the composite enzymatic assisted cell wall rupture process in the present invention has significant advantages in terms of cell wall degradation rate, oil extraction rate and functional component content compared with the single enzyme method in the comparative example. The cell wall degradation rate of the experimental group was 82.1%, which was 27% higher than the 64.8% of the comparative example. The oil extraction rate was increased from 74.3% of the comparative example to 87.2%, and the content of the functional components sesamol and sesamin was increased by 27.2% and 27.1%, respectively. These data fully verify the technical innovation and superiority of the present invention.

[0209] 1. Improvement of cell wall degradation rate

[0210] In the composite enzymatic process of the present invention, cellulase, pectinase and protease act synergistically to comprehensively degrade the multi-component structure of sesame cell walls:

[0211] Cellulase mainly degrades the cellulose backbone of the cell wall, weakening the overall strength of the cell wall.

[0212] Pectinase further breaks down pectin in the cell wall, which is the key adhesive of the cell wall. The degradation of pectin can relax the cell wall structure.

[0213] Proteases act on matrix proteins in the cell wall, further releasing bound lipids and functional ingredients.

[0214] Compared with the single enzyme method of the control group, which only relied on the action of cellulase, it failed to effectively degrade pectin and protein, resulting in limited cell wall rupture. The results showed that the cell wall degradation rate of the control group was only 64.8%, while that of the experimental group reached 82.1%, significantly improving the cell wall rupture effect.

[0215] 2. Improvement of oil extraction rate

[0216] The present invention releases more intracellular oil through compound enzyme treatment, thereby improving the availability of oil in subsequent extraction steps. The combined use of cellulase, pectinase and protease breaks the structural barrier of the cell wall and reduces the hindrance of cell wall residue to oil extraction.

[0217] In the comparative example, the single enzyme method had a limited scope of action and failed to fully release the bound oil. Although oil extraction was achieved to a certain extent, its extraction rate was only 74.3%. In contrast, the experimental group increased the extraction rate to 87.2% through the multiple synergistic effects of the composite enzyme, proving the significant advantages of the present invention in improving the extractability of oil.

[0218] 3. Increased retention of functional ingredients

[0219] Sesamol and sesamin are important functional components of sesame oil, and their retention rate directly affects the nutritional value and market competitiveness of sesame oil. The composite enzymatic process of the present invention avoids the destruction of functional components by high temperature or strong acid and alkali through mild enzymolysis conditions (temperature 45°C, pH 5.0), while releasing more bound sesamol and sesamin.

[0220] The contents of sesamol and sesamin in the experimental group reached 3.41 mg / g and 5.12 mg / g respectively, while those in the control group were only 2.68 mg / g and 4.03 mg / g. Due to insufficient treatment, the functional components of the single enzyme method were not fully released and may be partially lost.

[0221] The composite enzymatic technology of the present invention is based on the synergistic effect of cellulase, pectinase and protease, which innovatively solves the problem of limited action range of traditional single enzyme method. The introduction of pectinase and protease greatly enhances the destructive power of cell wall, while cellulase further enhances the overall degradation effect, forming a multi-level and multi-target degradation mechanism.

[0222] In Comparative Example 1, only cellulase was used. Although cellulose could be degraded to a certain extent, the pectin and protein matrix in the cell wall were not effectively degraded, resulting in the cell wall still having a high integrity, thereby limiting the release of oil and the extraction of functional ingredients.

[0223] Comparative experiment 2:

[0224] Objective: To verify the advantages of the solvent selection of the present invention, including the improvement of oil extraction efficiency, functional component retention rate and oil quality, by comparing the subcritical butane (experimental group, Example 1) and subcritical propane (experimental group, Example 2) used in the present invention with the traditional n-hexane solvent extraction process (Comparative Example 2).

[0225] Experimental setup:

[0226] Experimental group 1 (Example 1): subcritical butane was used as the solvent, the mass ratio of butane to sesame was 4:1, the extraction temperature was 35° C., the pressure was 0.3 MPa, and the time was 15 minutes.

[0227] Experimental group 2 (Example 2): subcritical propane was used as the solvent, the mass ratio of propane to sesame was 5:1, the extraction temperature was 40° C., the pressure was 0.5 MPa, and the time was 20 minutes.

[0228] Comparative Example 2: using traditional n-hexane solvent, the mass ratio of solvent to sesame is 4:1, the extraction temperature is 45°C, the pressure is normal, and the time is 30 minutes.

[0229] Experimental steps:

[0230] 1. Sample Preparation

[0231] 20 g of the crushed sesame samples were divided into groups, and the extraction system was configured according to the solvent type and ratio of the experimental group and the comparative example.

[0232] The extraction system includes crushed sesame seeds and corresponding solvents.

[0233] 2. Solvent Extraction

[0234] Each set of samples was placed in its own corresponding extraction device.

[0235] The experimental group used subcritical fluid extraction equipment to perform extraction at set temperature and pressure.

[0236] The comparative example used a conventional solvent extraction apparatus, operating at normal pressure and a relatively high temperature.

[0237] 3. Post-extraction processing

[0238] The extract was centrifuged (4000 rpm, 5 min) to separate the oil phase and the solvent phase.

[0239] The solvent is recovered using a vacuum distillation device to obtain a sesame oil product.

[0240] 4. Detection indicators

[0241] Extraction time (min): record the time required for each group to reach the set extraction conditions.

[0242] Oil extraction rate (%): the percentage of sesame oil mass to the total mass of the sample.

[0243] Functional ingredient retention rate (%): the ratio of the content of sesamol and sesamin to the total content in the raw material after being measured respectively.

[0244] Peroxide value (POV): The initial peroxide value (g / 100 g) of sesame oil was measured.

[0245] The experimental results are shown in the following table:

[0246]

[0247] This experiment verified the significant advantages of the subcritical fluid extraction technology of the present invention by comparing the effects of subcritical butane (experimental group 1) and subcritical propane (experimental group 2) used in the present invention with the traditional n-hexane extraction process (comparative example 2). The results showed that the extraction efficiency of experimental group 1 was the highest, and the functional component retention rate and oil quality were better than those of experimental group 2 and the comparative example. The comparative example had the lowest extraction efficiency, serious loss of functional components, and a significantly higher peroxide value, which further verified the innovation of the present invention in terms of solvent selection and optimization of extraction conditions.

[0248] 1. Shorten the extraction time

[0249] The experimental results show that the extraction time of experimental group 1 (butane) is only 15.2 minutes, experimental group 2 (propane) is 20.3 minutes, and the comparative example (n-hexane) takes 30.1 minutes. The low viscosity and high solubility of subcritical solvents (butane and propane) enable them to quickly dissolve sesame oil at lower temperatures and pressures, significantly shortening the extraction time.

[0250] In Example 1 of the present invention, butane can quickly penetrate sesame particles and efficiently dissolve oil under subcritical conditions due to its better solubility and lower boiling point (-0.5°C), and the extraction rate is much higher than that of traditional n-hexane.

[0251] In Comparative Example 2, the n-hexane extraction was carried out under normal pressure and high temperature (45° C.), and its dissolution efficiency was limited. Moreover, since it took a long time to complete the extraction, it was difficult to meet the needs of efficient industrial production.

[0252] 2. Improvement of oil extraction rate

[0253] The oil extraction rate of experimental group 1 was 87.0%, and that of experimental group 2 was 85.5%, both significantly higher than 73.2% of the comparative example. This is because the subcritical solvent used in the present invention can effectively avoid oil residue under low temperature and low pressure conditions:

[0254] The molecular structure of butane is more suitable for dissolving the non-polar and slightly polar components in sesame oil. It has strong permeability in the subcritical state (35°C, 0.3MPa), thus achieving efficient extraction.

[0255] Although propane in experimental group 2 is slightly inferior to butane, its higher solubility is still better than that of n-hexane, and the extraction efficiency is significantly improved.

[0256] The solubility of n-hexane in the comparative example is limited, and its high temperature operation easily leads to oil volatilization loss and oil waste in the residue.

[0257] 3. Increased retention of functional ingredients

[0258] The retention rates of the functional ingredients (sesamol and sesamin) in experimental group 1 and experimental group 2 were 92.1%-95.3% and 90.2%-94.0%, respectively, while the control group was only 75.0%-77.1%. The subcritical conditions in the present invention have a significant protective effect on the functional ingredients, mainly due to the following reasons:

[0259] Low temperature conditions: Subcritical butane and propane extractions are performed at low temperatures of 35°C to 40°C, which avoids the destruction of sesamol and sesamin by high temperature conditions. However, n-hexane extraction needs to be performed at 45°C, as higher temperatures lead to thermal degradation of functional ingredients.

[0260] Low-pressure environment: Subcritical solvent extraction does not require high pressure, and the interaction between solvent and oil is more gentle, reducing the mechanical stress loss of functional ingredients.

[0261] The low polarity and high permeability of butane in experimental group 1 enabled it to release bound sesamol and sesamin more effectively, thus achieving a higher retention rate during the extraction process.

[0262] 4. Reduction of oil peroxide value

[0263] The peroxide values ​​(POV) of experimental group 1 and experimental group 2 were 0.012g / 100g and 0.013g / 100g, respectively, which were much lower than 0.046g / 100g of the comparative example. The peroxide value of oil directly reflects the quality of oil. The low peroxide value of the present invention proves that the subcritical solvent extraction technology can significantly reduce the risk of oil oxidation:

[0264] The extraction conditions of butane and propane are low temperature and low pressure, which avoids the double bond oxidation and peroxide generation in oils and fats under high temperature conditions.

[0265] The high temperature operation of n-hexane in the comparative example resulted in the aggravated oxidation of sesame oil, a significant increase in the peroxide value, and a decrease in oil quality.

[0266] In addition, the solvent is better sealed during the subcritical solvent extraction process, which isolates the oxygen contact during the extraction process and further reduces the degree of oxidation of the oil.

[0267] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A sesame oil extraction process, characterized in that: The following steps are involved: Raw material pretreatment: washing, drying and crushing sesame seeds; Enzymatically assisted cell wall rupture: The broken sesame seeds are mixed with enzyme solution, and the sesame cell walls are enzymatically degraded using cellulase, pectinase and protease; Subcritical Fluid Extraction: Extraction of sesame oil under subcritical fluid conditions; Oil refining: The extracted sesame oil is subjected to physical filtration, enzymatic degumming and vacuum deodorization; Finished product packaging: Filling the refined sesame oil.

2. A sesame oil extraction process according to claim 1, characterized in that, The washing step in the raw material pretreatment comprises mixing sesame with water at a water to sesame mass ratio of 1:10-1:15, the water temperature is 25-35° C., and the washing time is 10-15 minutes.

3. A sesame oil extraction process according to claim 1, characterized in that, In the enzymatically assisted cell wall rupture step, the enzyme solution comprises: Cellulase, concentration 0.4%-0.6% (w / v); Pectinase, concentration 0.2%-0.4% (w / v); Protease, concentration 0.1%-0.3% (w / v); The mass ratio of enzyme solution to sesame is 1:2-1:3, the temperature of enzymatic reaction is 45-55°C, and the time is 60-90 minutes.

4. A sesame oil extraction process according to claim 3, characterized in that, The enzymatically assisted cell wall disruption step uses citric acid-sodium citrate buffer as a solvent, and the pH value of the buffer is 5.0-5.

5.

5. A sesame oil extraction process according to claim 1, characterized in that, In the subcritical fluid extraction step, subcritical butane is used as a solvent, the mass ratio of butane to sesame is 4:1-6:1, the extraction temperature is 35-45° C., the extraction pressure is 0.3-0.6 MPa, and the extraction time is 15-25 minutes.

6. A sesame oil extraction process according to claim 5, characterized in that: After the subcritical fluid extraction step is completed, butane is recovered by vacuum distillation equipment, with a recovery rate of not less than 95%, a distillation temperature of 40-50° C., and a vacuum degree of 0.05-0.1 MPa.

7. A sesame oil extraction process according to claim 1, characterized in that: The oil refining step comprises: Physical filtration through a filter membrane with a pore size of 0.2-0.5 μm; Adding phospholipase for enzymatic degumming, the concentration of phospholipase is 0.1%-0.2% (w / v), the reaction temperature is 25-30°C, and the reaction time is 30-60 minutes; The sesame oil is deodorized by a low-temperature vacuum deodorization device, the deodorization temperature is 50-60°C, and the vacuum degree is 0.05-0.1MPa.

8. A sesame oil extraction process according to claim 7, characterized in that: In the enzymatic degumming step, sesame oil and deionized water are mixed in a mass ratio of 1:10-1:15, and separated by a centrifuge to obtain refined oil.

9. A sesame oil extraction process according to claim 7, characterized in that: In the vacuum deodorization step, low-temperature steam is used as the deodorization medium, the steam flow rate is 1-2 L / min, and the deodorization time is 20-30 minutes.

10. A sesame oil extraction process according to claim 1, characterized in that: The finished product packaging step adopts a light-proof food-grade container, the filling temperature is 20-25° C., and the container storage conditions are a temperature of 15-25° C. and a humidity of less than 60%.

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