Multistage tandem continuous enzymolysis reaction equipment and enzymolysis method

Abstract

The invention provides multistage tandem continuous enzymolysis reaction equipment and an enzymolysis method, and belongs to the technical field of biochemical engineering. The equipment comprises a belt conveyor, a screw conveyor, a batching tank and at least one enzymolysis tank which are connected in sequence, and the control system is used for controlling the mixing tank and the enzymolysis tank to continuously operate and work. Materials enter a batching tank through a belt conveyor and a screw conveyor, a control system calculates the water feeding mass and the enzyme preparation feeding amount according to the amount of the added materials and controls the water feeding mass and the enzyme preparation feeding amount to enter the batching tank, and in the batching process, the control system automatically controls the temperature, the pH value and the liquid level in the batching tank in real time; the control system controls ingredient liquid to enter the enzymolysis tank according to the liquid level condition in the ingredient tank, the control system automatically controls the temperature, the pH value and the liquid level in the enzymolysis tank in real time in the enzymolysis process, continuous enzymolysis reaction of equipment is achieved, the utilization rate of the equipment is increased, time is saved, and the productivity is improved.

Description

Technical Field

[0001] The invention belongs to the technical field of biochemical engineering, and in particular relates to a multi-stage series continuous enzymolysis reaction device and an enzymolysis method. Background Art

[0002] As a raw material for the production of bio-based chemicals, sugar is as important as "ethylene" in petrochemical products. Developing sugar production technology using agricultural waste such as straw as raw materials can not only avoid threats to food security, but also reduce the cost of sugar, creating a good development space for the development and production of bio-based chemicals.

[0003] The main methods of making sugar from straw raw materials are acid hydrolysis and enzymatic hydrolysis. Acid hydrolysis has a series of problems such as equipment corrosion, harsh process conditions and difficult wastewater treatment. Enzymatic hydrolysis process conditions are mild, so enzymatic hydrolysis has become a research hotspot for making sugar from straw raw materials. At present, enzymatic hydrolysis generally adopts an intermittent operation method, that is, each batch of enzymatic hydrolysis production needs to go through four processes such as feeding, reaction, discharging and equipment cleaning, which has a low level of automation, resulting in low production efficiency and time-consuming problems. Summary of the invention

[0004] The purpose of the present invention is to solve the above-mentioned problems existing in the prior art, and to provide a multi-stage series continuous enzymatic hydrolysis reaction equipment and an enzymatic hydrolysis method, so as to realize the continuous operation of the enzymatic hydrolysis reaction and improve the production efficiency.

[0005] The present invention is achieved through the following technical solutions:

[0006] The first aspect of the present invention provides a multi-stage series continuous enzymolysis reaction equipment, comprising a belt conveyor, a screw conveyor, a batching tank and at least one enzymolysis tank connected in sequence;

[0007] It also includes a control system for controlling the continuous operation of the batching tank and the enzymolysis tank.

[0008] A further improvement of the present invention is:

[0009] The control system includes a batching control unit and an enzymatic hydrolysis control unit;

[0010] The batching control unit includes a calculation module and a feeding control module;

[0011] The calculation module includes a first calculation module and a second calculation module, wherein the first calculation module is used to calculate the mass flow rate of the upper water according to the mass flow rate of the material entering the batching tank, and the second calculation module is used to calculate the mass flow rate of the enzyme preparation according to the mass flow rate of the material entering the batching tank;

[0012] The feed control module includes a first feed control module and a second feed control module. The first feed control module is connected to the first calculation module and is used to control the mass flow of water entering the batching tank according to the calculation results of the first calculation module. The second feed control module is connected to the second calculation module and is used to control the mass flow of enzyme preparation entering the batching tank according to the calculation results of the second calculation module.

[0013] A further improvement of the present invention is:

[0014] The batching control unit also includes a first temperature control module, a first pH control module and a first liquid level control module. The first temperature control module is used to control the temperature in the batching tank to be at a set temperature value; the first pH control module is used to control the pH value in the batching tank to be at a set pH value; the first liquid level control module is used to control the liquid level in the batching tank to be at a set liquid level value.

[0015] A further improvement of the present invention is:

[0016] The enzymolysis control unit includes a second temperature control module, a second pH control module and a second liquid level control module. The second temperature control module is used to control the temperature in the enzymolysis tank to a set temperature value; the second pH control module is used to control the pH value in the enzymolysis tank to a set pH value; the second liquid level control module is used to control the liquid level in the enzymolysis tank to a set liquid level value.

[0017] A further improvement of the present invention is:

[0018] The top of the batching tank is connected to a water supply pipeline, and a first mass flow transmitter and a water supply regulating valve are arranged on the water supply pipeline, and the first mass flow transmitter and the water supply regulating valve are both connected to the first feed control module;

[0019] The top of the batching tank is also connected to a variable frequency batching pump through a pipeline, and the pipeline is also connected to a second mass flow transmitter. The variable frequency batching pump and the second mass flow transmitter are both connected to the second feed control module.

[0020] A further improvement of the present invention is:

[0021] A first circulating water system is arranged on the outer wall of the batching tank, a water inlet of the first circulating water system is connected to an external hot water supply pipe through a pipe, a first hot water valve is arranged on the pipe connecting the water inlet and the external hot water supply pipe, and the first hot water valve is connected to a first temperature control module;

[0022] The batching tank is also provided with a first temperature transmitter connected to the first temperature control module.

[0023] A further improvement of the present invention is:

[0024] The top of the batching tank is also connected to a first liquid alkali pipeline, on which a first liquid alkali valve is provided, and the first liquid alkali valve is connected to a first pH control module;

[0025] The batching tank is also provided with a first pH meter connected to the first pH control module.

[0026] A further improvement of the present invention is:

[0027] The bottom of the batching tank is provided with a discharge port, the discharge port is connected to a first batching pump through a pipeline, the first batching pump is connected to a reflux port provided at the top of the batching tank through a first pipeline, the first batching pump is connected to the enzymolysis tank through a second pipeline, and the second pipeline is provided with a first liquid level regulating valve;

[0028] The batching tank is provided with a first liquid level transmitter, and the first liquid level control module is respectively connected to the first liquid level regulating valve, the first liquid level transmitter and the control switch of the belt conveyor.

[0029] A further improvement of the present invention is:

[0030] A second circulating water system is provided on the outer wall of the enzymolysis tank, the water inlet of the second circulating water system is connected to an external hot water supply pipe through a pipe, a second hot water regulating valve is provided on the pipe connecting the water inlet and the external hot water supply pipe, and the second hot water regulating valve is connected to the second temperature control module;

[0031] The enzymolysis tank is also provided with a second temperature transmitter connected to the second temperature control module.

[0032] A further improvement of the present invention is:

[0033] The top of the enzymolysis tank is also connected to a second liquid alkali pipeline, on which a second liquid alkali valve is provided, and the second liquid alkali valve is connected to a second pH control module;

[0034] The enzymolysis tank is also provided with a second pH meter connected to the second pH control module.

[0035] A further improvement of the present invention is:

[0036] The bottom of the enzymolysis tank is provided with a discharge port, the discharge port is connected to the second dosing pump through a pipeline, the second dosing pump is connected to the reflux port provided on the upper part of the enzymolysis tank through the first delivery pipeline, the second dosing pump is connected to the next-level enzymolysis tank or storage tank through the second delivery pipeline, and a second liquid level regulating valve is provided on the second delivery pipeline, and the second liquid level regulating valve is connected to the second liquid level control module;

[0037] A second liquid level transmitter is provided in the enzymolysis tank, and the second liquid level transmitter is connected to the second liquid level control module.

[0038] The second aspect of the present invention provides a multi-stage series continuous enzymolysis method, which is carried out using the multi-stage series continuous enzymolysis reaction equipment, and the method includes a batching process and an enzymolysis process.

[0039] (I) The batching process specifically includes:

[0040] The pre-treated materials are continuously transported to the batching tank through belt conveyors and screw conveyors;

[0041] The control system calculates the mass flow rate of the feed water and the mass flow rate of the enzyme preparation according to the flow rate of the pre-treated material entering the batching tank, and controls the feed water and the enzyme preparation to enter the batching tank;

[0042] During the batching process, the control system automatically adjusts the temperature, pH value and liquid level in the batching tank in real time;

[0043] (II) The enzymatic hydrolysis process specifically includes:

[0044] The batching liquid in the batching tank is input into the enzymolysis tank through the control system to finally produce enzymolysis liquid. During the enzymolysis process, the control system automatically adjusts the temperature, pH value and liquid level in the enzymolysis tank in real time.

[0045] A further improvement of the present invention is:

[0046] The calculation formula for the water mass flow rate is:

[0047]

[0048] The enzyme preparation mass flow calculation formula is:

[0049] Enzyme preparation mass flow rate = pretreatment material mass flow rate × enzyme addition ratio.

[0050] Compared with the prior art, the present invention has the following beneficial effects:

[0051] The invention discloses a multi-stage series continuous enzymolysis device. Materials are conveyed to a screw conveyor through a belt conveyor, and then enter a batching tank through a discharge port of the screw conveyor. A control system calculates the quality of water and the feed amount of enzyme preparation according to the amount of added materials, and then controls the addition of the materials into the batching tank for batching according to the calculation results. During the batching process, the control system automatically controls the temperature, pH value and liquid level in the batching tank in real time. The control system controls the materials to enter an enzymolysis tank for enzymolysis according to the liquid level in the batching tank. During the enzymolysis process, the control system automatically controls the temperature, pH value and liquid level in the enzymolysis tank in real time.

[0052] The present invention can reduce cumbersome and complicated operating procedures for operators, can realize continuous enzymatic hydrolysis reaction of equipment, increase equipment utilization, save time and improve productivity. BRIEF DESCRIPTION OF THE DRAWINGS

[0053] Figure 1 It is a schematic diagram of the structure of a multi-stage series continuous enzymatic hydrolysis reaction device in an embodiment of the present invention;

[0054] Figure 2 is a schematic diagram of the structure of a control system in an embodiment of the present invention;

[0055] Figure 3 This is a schematic diagram of the connection between the batching tank and the control system in an embodiment of the present invention;

[0056] Figure 4 This is a schematic diagram of the connection between the enzymatic hydrolysis tank and the control system in an embodiment of the present invention.

[0057] In the figure, 1. belt conveyor, 2. screw conveyor, 3. batching tank, 4. enzymatic hydrolysis tank, 5. control system, 6. frequency conversion pump, 7. first batching pump, 8. second batching pump. DETAILED DESCRIPTION

[0058] The present invention is further described in detail below in conjunction with the accompanying drawings:

[0059] [Example 1]

[0060] This embodiment proposes a multi-stage series continuous enzymatic reaction device, such as Figure 1 As shown, it includes a belt conveyor 1, a screw conveyor 2, a batching tank 3 and at least one enzymolysis tank 4 connected in sequence, and also includes a control system 5 for controlling the batching tank 3 and the enzymolysis tank 4 to operate continuously.

[0061] The material is conveyed to the screw conveyor 2 by the belt conveyor 1, and then enters the batching tank 3 through the discharge port of the screw conveyor 2. The control system 5 calculates the quality of the water and the feed amount of the enzyme preparation according to the amount of the added material, and then controls the addition of the materials into the batching tank 3 for batching according to the calculation results. During the batching process, the control system automatically controls the temperature, pH value and liquid level in the batching tank 3 in real time; the control system 5 controls the material to enter the enzymolysis tank 4 for enzymolysis according to the liquid level in the batching tank 3. During the enzymolysis process, the control system automatically controls the temperature, pH value and liquid level in the enzymolysis tank in real time.

[0062] Among them, the belt conveyor 1 includes a weighing module and a control switch for controlling the belt conveyor 1 to be turned on or off. The weighing module is used to measure the mass flow rate of the material flowing through the belt conveyor 1. The belt conveyor 1 and the screw conveyor 2 both use existing technical equipment, which will not be repeated here.

[0063] [Example 2]

[0064] like Figure 2 As shown, the control system includes a batching control unit and an enzymatic hydrolysis control unit.

[0065] The batching control unit includes a calculation module and a feeding control module, the calculation module includes a first calculation module and a second calculation module, the first calculation module and the second calculation module are both connected to the weighing module on the belt conveyor, the first calculation module is used to calculate the mass flow rate of water according to the mass flow rate of the material entering the batching tank 3 (that is, the mass flow rate of the material measured by the weighing module on the belt conveyor 1), and the second calculation module is used to calculate the mass flow rate of the enzyme preparation according to the mass flow rate of the material entering the batching tank 3 (that is, the mass flow rate of the material measured by the weighing module on the belt conveyor 1);

[0066] The feed control module includes a first feed control module and a second feed control module. The first feed control module is connected to the first calculation module and is used to control the mass flow rate of water entering the batching tank 3 according to the calculation results of the first calculation module. The second feed control module is connected to the second calculation module and is used to control the mass flow rate of enzyme preparation entering the batching tank 3 according to the calculation results of the second calculation module.

[0067] The batching control unit also includes a first temperature control module, a first pH control module and a first liquid level control module. The first temperature control module is used to control the temperature in the batching tank 3 to be at a set temperature value, so that the temperature in the batching tank 3 is always at an optimal temperature; the first pH control module is used to control the pH value in the batching tank 3 to be at a set pH value, so that the temperature in the batching tank 3 is always at an optimal pH value; the first liquid level control module is used to control the liquid level in the batching tank 3 to be at a set liquid level value.

[0068] The enzymolysis control unit includes a second temperature control module, a second pH control module and a second liquid level control module. The second temperature control module is used to control the temperature in the enzymolysis tank 4 to be at a set temperature value, so that the temperature in the enzymolysis tank 4 is always at an optimal temperature; the second pH control module is used to control the pH value in the enzymolysis tank 4 to be at a set pH value, so that the temperature in the enzymolysis tank 4 is always at an optimal pH value; the second liquid level control module is used to control the liquid level in the enzymolysis tank 4 to be at a set liquid level value.

[0069] The number of enzymatic hydrolysis control units is the same as the number of enzymatic hydrolysis tanks, one to one, and the number of enzymatic hydrolysis tanks is determined according to the material reaction time and output.

[0070] [Example 3]

[0071] like Figure 3As shown, a water supply pipeline is connected to the top of the batching tank 3, and a first mass flow transmitter and a water supply regulating valve are arranged on the water supply pipeline. The first mass flow transmitter is used to monitor the water supply mass flow flowing through the water supply pipeline in real time. The first mass flow transmitter and the water supply regulating valve are both connected to the first feed control module. The first feed control module judges the calculation result of the first calculation module and the size of the monitoring result of the first mass flow transmitter, and controls the opening and closing size of the water supply regulating valve according to the judgment result, thereby controlling the water supply mass flow. Specifically, if the monitoring result of the first mass flow transmitter is less than the calculation result of the first calculation module, the first feed control module controls the water supply regulating valve to open wider, thereby increasing the water supply mass flow entering the batching tank 3; if the monitoring result of the first mass flow transmitter is greater than the calculation result of the first calculation module, the first feed control module controls the water supply regulating valve to close smaller, thereby reducing the water supply mass flow entering the batching tank 3.

[0072] Furthermore, a variable frequency dosing pump 6 is connected to the top of the dosing tank 3 through a pipeline, and a second mass flow transmitter is also connected to the pipeline. The second mass flow transmitter is used to monitor the mass flow of the enzyme preparation entering the dosing tank 3 in real time. The variable frequency dosing pump 6 and the second mass flow transmitter are both connected to the second feed control module. The second feed control module judges the calculation result of the second calculation module and the monitoring result of the second mass flow transmitter, and controls the frequency of the variable frequency dosing pump 6 according to the judgment result, thereby controlling the mass flow of the enzyme preparation. Specifically, if the monitoring result of the second mass flow transmitter is less than the calculation result of the second calculation module, the second feed control module controls the frequency of the variable frequency dosing pump 6 to increase, thereby increasing the mass flow of the enzyme preparation entering the dosing tank 3; if the monitoring result of the second mass flow transmitter is greater than the calculation result of the second calculation module, the second feed control module controls the frequency of the variable frequency dosing pump 6 to decrease, thereby reducing the mass flow of the enzyme preparation entering the dosing tank 3.

[0073] Furthermore, a first circulating water system is arranged on the outer wall of the batching tank 3, the water inlet of the first circulating water system is connected to an external hot water supply pipe through a pipe, the water outlet of the first circulating water system is connected to an external pipe, a first hot water valve is arranged on the pipe connecting the water inlet with the external hot water supply pipe, the first hot water valve is connected to a first temperature control module, the opening and closing size of the first hot water valve is controlled by the first temperature control module, and then the amount of hot water entering the first circulating water system is controlled; a first temperature transmitter connected to the first temperature control module is also arranged in the batching tank 3, the first temperature transmitter monitors the temperature in the batching tank 3 in real time, when the temperature in the batching tank 3 is lower than the set value, the first temperature control module opens the first hot water valve to increase the hot water flow, when the temperature in the batching tank 3 is higher than the set value, the first temperature control module closes the first hot water valve to reduce the hot water flow, so that the temperature in the batching tank 3 is always at an optimal temperature.

[0074] Furthermore, a first liquid alkali pipeline is also connected to the top of the batching tank 3, and a first liquid alkali valve is arranged on the first liquid alkali pipeline. The first liquid alkali valve is connected to a first pH control module, and the opening and closing size of the first liquid alkali valve is controlled by the first pH control module to adjust the pH value in the batching tank 3; a first pH meter connected to the first pH control module is also provided in the batching tank 3, and the first pH meter monitors the pH value in the batching tank 3 in real time. When the pH value in the batching tank 3 is lower than the set value, the first pH control module opens the first liquid alkali valve to increase the liquid alkali flow rate. When the pH value in the batching tank 3 is higher than the set value, the first pH control module closes the first liquid alkali valve to reduce the liquid alkali flow rate, so that the pH value in the batching tank 3 is always at the best.

[0075] Furthermore, a discharge port is provided at the bottom of the batching tank 3, and a first batching pump 7 is connected to the discharge port through a pipeline. During the operation of the equipment, the first batching pump 7 is always in an open state, and the first batching pump 7 is connected to the reflux port provided at the upper part of the batching tank 3 through a first pipeline, and the first batching pump 7 is connected to the enzymolysis tank 4 through a second pipeline, and a first liquid level regulating valve is provided on the second pipeline. A first liquid level transmitter is provided in the batching tank 3, and the first liquid level transmitter is used to monitor the liquid level value in the batching tank 3 in real time; the first liquid level control module is respectively connected to the first liquid level regulating valve, the first liquid level transmitter and the control switch of the belt conveyor 1. If the first liquid level transmitter monitors that the liquid level value in the batching tank 3 is less than the minimum liquid level setting value, the first liquid level regulating valve is controlled to be closed. At this time, the belt conveyor 1 is in a working state, so that the batching liquid flowing out of the discharge port of the batching tank 3 passes through the first batching pump 7 in sequence. , the first pipeline and the reflux port of the batching tank 3 flow back to the batching tank 3; if the first liquid level transmitter monitors that the liquid level value in the batching tank 3 is between the minimum liquid level setting value and the maximum liquid level setting value, the first liquid level control module controls the first liquid level regulating valve to open, and at this time the belt conveyor 1 is in working state, so that the batching liquid flowing out of the discharge port of the batching tank 3 flows into the enzymolysis tank 4 through the first batching pump 7 and the second pipeline in sequence; if the first liquid level transmitter monitors that the liquid level value in the batching tank 3 is greater than the maximum liquid level setting value, the first liquid level control module controls the first liquid level regulating valve to open, and at the same time controls the control switch of the belt conveyor to close, at this time the belt conveyor is in a closed state, and feeding is stopped until the first liquid level transmitter monitors that the liquid level value in the batching tank 3 is less than the minimum liquid level setting value, and the first liquid level control module controls the control switch of the belt conveyor to open, and continues feeding.

[0076] [Example 4]

[0077] like Figure 4As shown, a second circulating water system is arranged on the outer wall of the enzymolysis tank 4, the water inlet of the second circulating water system is connected to an external hot water supply pipe through a pipe, the water outlet of the second circulating water system is connected to an external pipe, a second hot water regulating valve is arranged on the pipe connecting the water inlet with the external hot water supply pipe, the second hot water regulating valve is connected to a second temperature control module, the opening and closing size of the second hot water regulating valve is controlled by the second temperature control module, and then the amount of hot water entering the second circulating water system is controlled; a second temperature transmitter connected to the second temperature control module is also provided in the enzymolysis tank 4, the second temperature transmitter monitors the temperature in the enzymolysis tank 4 in real time, when the temperature in the enzymolysis tank 4 is lower than the set value, the second temperature control module opens the second hot water regulating valve to increase the hot water flow, when the temperature in the enzymolysis tank 4 is higher than the set value, the second temperature control module closes the second hot water regulating valve to reduce the hot water flow, so that the temperature in the enzymolysis tank 4 is always at an optimal temperature.

[0078] Furthermore, a second liquid alkali pipeline is connected to the top of the enzymolysis tank 4, and a second liquid alkali valve is arranged on the second liquid alkali pipeline. The second liquid alkali valve is connected to a second pH control module, and the opening and closing size of the second liquid alkali valve is controlled by the second pH control module to adjust the pH value in the enzymolysis tank 4; a second pH meter connected to the second pH control module is also provided in the enzymolysis tank 4, and the second pH meter monitors the pH value in the enzymolysis tank 4 in real time. When the pH value in the enzymolysis tank 4 is lower than the set value, the second pH control module opens the second liquid alkali valve to increase the liquid alkali flow rate. When the pH value in the enzymolysis tank 4 is higher than the set value, the second pH control module closes the second liquid alkali valve to reduce the liquid alkali flow rate, so that the pH value in the enzymolysis tank 4 is always at the best.

[0079] Furthermore, a discharge port is provided at the bottom of the enzymolysis tank 4, and the discharge port is connected to the second dosing pump 8 through a pipeline. During the operation of the equipment, the second dosing pump is in an open state, and the second dosing pump 8 is connected to the reflux port provided on the upper part of the enzymolysis tank 4 through the first conveying pipeline. The second dosing pump 8 is connected to the next-level enzymolysis tank or storage tank through the second conveying pipeline, and a second liquid level regulating valve is provided on the second conveying pipeline, and the second liquid level regulating valve is connected to the second liquid level control module. The second liquid level control module controls the liquid level in the enzymolysis tank 4 by controlling the opening and closing of the second liquid level regulating valve; a second liquid level transmitter is provided in the enzymolysis tank 4, and the second liquid level transmitter is connected to the second liquid level control module The second liquid level transmitter monitors the liquid level in the enzymolysis tank 4 in real time. If the liquid level value in the enzymolysis tank 4 monitored by the second liquid level transmitter is less than the set value, the second liquid level control module controls the second liquid level regulating valve to close, so that the enzymolysis liquid flowing out of the discharge port of the enzymolysis tank 4 flows back to the enzymolysis tank 4 through the second dosing pump, the first conveying pipeline and the reflux port of the enzymolysis tank 4 in turn; if the liquid level value in the enzymolysis tank 4 monitored by the second liquid level transmitter is greater than the set value, the second liquid level control module controls the second liquid level regulating valve to open, so that the enzymolysis liquid flowing out of the discharge port of the enzymolysis tank 4 flows into the next-level enzymolysis tank or storage tank through the second dosing pump and the second conveying pipeline in turn.

[0080] [Example 5]

[0081] This embodiment provides a multi-stage series continuous enzymolysis reaction method, which is carried out using the multi-stage series continuous enzymolysis reaction equipment described in Examples 1-4. The method includes a batching process and an enzymolysis process.

[0082] (I) The batching process specifically includes:

[0083] The pre-treated materials are continuously conveyed to the batching tank 3 through the belt conveyor 1 and the screw conveyor 2;

[0084] The control system 5 calculates the mass flow rate of the water supply and the mass flow rate of the enzyme preparation according to the flow rate of the pretreated material entering the batching tank 3;

[0085] The calculation formula for the water mass flow rate is:

[0086]

[0087] Among them, the dry matter concentration of the pre-treated material is the concentration obtained by analysis and testing, and the dry matter concentration of the batching material refers to the concentration that the batching tank needs to reach. The dry matter concentration of the pre-treated material and the dry matter concentration of the batching material are both set in advance in the control system; the mass flow rate of the pre-treated material is directly measured by the weighing module on the belt conveyor;

[0088] The enzyme preparation mass flow rate calculation formula is:

[0089] Enzyme preparation mass flow rate = pretreatment material mass flow rate × enzyme addition ratio

[0090] During the batching process, the first feed control module determines the size of the calculation result of the first calculation module and the monitoring result of the first mass flow transmitter, and controls the opening and closing size of the water supply regulating valve according to the judgment result, thereby controlling the water supply mass flow rate; the second feed control module determines the size of the calculation result of the second calculation module and the monitoring result of the second mass flow transmitter, and controls the frequency of the variable frequency batching pump 6 according to the judgment result, thereby controlling the mass flow rate of the enzyme preparation, and at the same time, the stirring device arranged in the batching tank 3 stirs the material, and the control system 5 automatically adjusts the temperature, pH value and liquid level in the batching tank 3 in real time.

[0091] Among them, the control system 5 automatically adjusts the temperature, pH value and liquid level in the batching tank 3 in real time, specifically:

[0092] The first temperature transmitter monitors the temperature in the batching tank 3 in real time. When the temperature in the batching tank 3 is lower than the set value, the first temperature control module opens the first hot water valve to increase the hot water flow rate. When the temperature in the batching tank 3 is higher than the set value, the first temperature control module closes the first hot water valve to reduce the hot water flow rate.

[0093] The first pH meter monitors the pH value in the batching tank 3 in real time. When the pH value in the batching tank 3 is lower than the set value, the first pH control module opens the first liquid alkali valve to increase the liquid alkali flow rate. When the pH value in the batching tank 3 is higher than the set value, the first pH control module closes the first liquid alkali valve to reduce the liquid alkali flow rate.

[0094] The first liquid level transmitter is used to monitor the liquid level value in the batching tank 3 in real time. The first liquid level control module is respectively connected to the first liquid level regulating valve, the first liquid level transmitter and the control switch of the belt conveyor 1. If the first liquid level transmitter monitors that the liquid level value in the batching tank 3 is less than the minimum liquid level setting value, the first liquid level regulating valve is controlled to be closed. At this time, the belt conveyor 1 is in a working state, so that the batching liquid flowing out of the discharge port of the batching tank 3 flows back to the batching tank 3 through the first batching pump 7, the first pipeline and the reflux port of the batching tank 3 in turn; if the first liquid level transmitter monitors that the liquid level value in the batching tank 3 is between the minimum liquid level setting value and the maximum liquid level setting value, the first liquid level control The module controls the first liquid level regulating valve to open, at which time the belt conveyor 1 is in working condition, so that the batching liquid flowing out from the discharge port of the batching tank 3 flows into the enzymolysis tank 4 through the first batching pump 7 and the second pipeline in turn; if the first liquid level transmitter monitors that the liquid level value in the batching tank 3 is greater than the maximum liquid level setting value, the first liquid level control module controls the first liquid level regulating valve to open, and at the same time controls the control switch of the belt conveyor to close. At this time, the belt conveyor is in a closed state and stops feeding. Until the first liquid level transmitter monitors that the liquid level value in the batching tank 3 is less than the minimum liquid level setting value, the first liquid level control module controls the control switch of the belt conveyor to open and continue feeding.

[0095] (II) The enzymatic hydrolysis process specifically includes:

[0096] The dosing liquid from the dosing tank 3 is input into the enzymolysis tank 4 through the first dosing pump, and finally an enzymolysis liquid is produced. During the enzymolysis process, the control system 5 automatically adjusts the temperature, pH value and liquid level in the enzymolysis tank 4 in real time, specifically:

[0097] The second temperature transmitter monitors the temperature in the enzymolysis tank 4 in real time. When the temperature in the enzymolysis tank 4 is lower than the set value, the second temperature control module opens the second hot water valve to increase the hot water flow rate. When the temperature in the enzymolysis tank 4 is higher than the set value, the second temperature control module closes the second hot water valve to reduce the hot water flow rate.

[0098] The second pH meter monitors the pH value in the enzymolysis tank 4 in real time. When the pH value in the enzymolysis tank 4 is lower than the set value, the second pH control module opens the second liquid alkali valve to increase the liquid alkali flow rate. When the pH value in the enzymolysis tank 4 is higher than the set value, the second pH control module closes the second liquid alkali valve to reduce the liquid alkali flow rate.

[0099] The second liquid level transmitter monitors the liquid level in the enzymolysis tank 4 in real time. If the liquid level value in the enzymolysis tank 4 monitored by the second liquid level transmitter is less than the set value, the second liquid level control module controls the second liquid level regulating valve to close, so that the enzymolysis liquid flowing out of the discharge port of the enzymolysis tank 4 flows back to the enzymolysis tank 4 through the second dosing pump, the first conveying pipeline and the reflux port of the enzymolysis tank 4 in turn; if the liquid level value in the enzymolysis tank 4 monitored by the second liquid level transmitter is greater than the set value, the second liquid level control module controls the second liquid level regulating valve to open, so that the enzymolysis liquid flowing out of the discharge port of the enzymolysis tank 4 flows into the next-level enzymolysis tank or storage tank through the second dosing pump and the second conveying pipeline in turn.

[0100] The enzymatic reaction equipment and method of the present invention are further explained below through specific examples.

[0101] [Example 6]

[0102] A multi-stage series continuous enzymatic hydrolysis reaction equipment provided in Examples 1-4 is used to enzymatically hydrolyze the straw, wherein the enzymatic hydrolysis tanks are three series-connected enzymatic hydrolysis tanks, and the structures of the three enzymatic hydrolysis tanks and the structure of the enzymatic hydrolysis control unit used to control the automatic and continuous operation of the three enzymatic hydrolysis tanks are the same, and the control parameters such as temperature, pH and liquid level in the three enzymatic hydrolysis tanks are also the same.

[0103] The method is specifically as follows:

[0104] (I) Batching process

[0105] The pretreated material is continuously conveyed to the enzymolysis batching tank 3 through the belt conveyor 1 and the screw conveyor 2 at a rate of 2 t / h. At the same time, the control system 5 calculates the water mass flow rate and the enzyme preparation mass flow rate according to the pretreated straw mass flow rate, and controls them to continuously enter the batching tank 3, wherein the dry matter concentration of the pretreated material is 20%, the dry matter concentration of the batching is 10%, and the enzyme addition ratio is set to 2;

[0106] During the batching process, the control system 5 is automatically adjusted in real time, wherein the temperature setting value in the batching tank 3 is 45°C, the pH setting value is 4, the maximum liquid level setting value is 90% and the minimum liquid level setting value is 70%, specifically:

[0107] The first temperature transmitter monitors the temperature in the batching tank 3 in real time. When the temperature in the batching tank 3 is lower than 45°C, the first temperature control module opens the first hot water valve to increase the hot water flow rate. When the temperature in the batching tank 3 is higher than 45°C, the first temperature control module closes the first hot water valve to reduce the hot water flow rate.

[0108] The first pH meter monitors the pH value in the batching tank 3 in real time. When the pH value in the batching tank 3 is lower than 4, the first pH control module opens the first liquid alkali valve to increase the liquid alkali flow rate. When the pH value in the batching tank 3 is higher than 4, the first pH control module closes the first liquid alkali valve to reduce the liquid alkali flow rate.

[0109] The first liquid level transmitter is used to monitor the liquid level value in the batching tank 3 in real time. The first liquid level control module is connected to the first liquid level regulating valve, the first liquid level transmitter and the control switch of the belt conveyor 1 respectively. If the first liquid level transmitter monitors that the liquid level value in the batching tank 3 is less than 70%, the first liquid level regulating valve is controlled to be closed. At this time, the belt conveyor 1 is in working state, so that the batching liquid flowing out of the discharge port of the batching tank 3 flows back to the batching tank 3 through the first batching pump 7, the first pipeline and the reflux port of the batching tank 3 in turn; if the first liquid level transmitter monitors that the liquid level value in the batching tank 3 is greater than 70% and less than 90%, the first liquid level control module controls The first liquid level regulating valve is opened, and the belt conveyor 1 is in working condition at this time, so that the batching liquid flowing out from the discharge port of the batching tank 3 flows into the enzymolysis tank 4 through the first batching pump 7 and the second pipeline in turn; if the first liquid level transmitter monitors that the liquid level value in the batching tank 3 is greater than 90%, the first liquid level control module controls the first liquid level regulating valve to open, and at the same time controls the control switch of the belt conveyor to close. At this time, the belt conveyor is in a closed state and stops feeding. Until the first liquid level transmitter monitors that the liquid level value in the batching tank 3 is less than the minimum liquid level setting value, the first liquid level control module controls the control switch of the belt conveyor to open and continue feeding.

[0110] (II) Enzymatic hydrolysis process

[0111] The liquid from the batching tank 3 is input into the first-stage enzymolysis tank through the first batching pump, and finally an enzymolysis solution is produced. During the enzymolysis process, the control system 5 is automatically adjusted in real time to control the temperature in the three enzymolysis tanks to be 45°C, the pH value to be 4, and the liquid level to be 70% of the enzymolysis tank, specifically:

[0112] The second temperature transmitter monitors the temperature in the enzymolysis tank 4 in real time. When the temperature in the enzymolysis tank 4 is lower than 45°C, the second temperature control module opens the second hot water valve to increase the hot water flow rate. When the temperature in the enzymolysis tank 4 is higher than 45°C, the second temperature control module closes the second hot water valve to reduce the hot water flow rate.

[0113] The second pH meter monitors the pH value in the enzymolysis tank 4 in real time. When the pH value in the enzymolysis tank 4 is lower than 4, the second pH control module opens the second liquid alkali valve to increase the liquid alkali flow rate. When the pH value in the enzymolysis tank 4 is higher than 4, the second pH control module closes the second liquid alkali valve to reduce the liquid alkali flow rate.

[0114] The second liquid level transmitter monitors the liquid level in the enzymolysis tank 4 in real time. If the liquid level value in the enzymolysis tank 4 monitored by the second liquid level transmitter is less than 70%, the second liquid level control module controls the second liquid level regulating valve to close, so that the enzymolysis liquid flowing out of the discharge port of the enzymolysis tank 4 flows back to the enzymolysis tank 4 through the second dosing pump, the first conveying pipeline and the reflux port of the enzymolysis tank 4 in turn; if the liquid level value in the enzymolysis tank 4 monitored by the second liquid level transmitter is greater than 70%, the second liquid level control module controls the second liquid level regulating valve to open, so that the enzymolysis liquid flowing out of the discharge port of the enzymolysis tank 4 flows into the next-level enzymolysis tank or storage tank through the second dosing pump and the second conveying pipeline in turn.

[0115] In this embodiment, the total residence time of the batching tank and the three enzymolysis tanks is controlled at 48 hours.

[0116] [Example 7]

[0117] The equipment used in Example 7 is the same as that in Example 6, and the difference in the enzymatic hydrolysis process is that in Example 7, the mass flow rate of the pretreated material is 5 t / h, the dry matter concentration of the pretreated material is set to 50%, the dry matter concentration of the ingredients is set to 30%, and the enzyme addition ratio is set to 20;

[0118] During the batching process, the control system 5 is automatically adjusted in real time, wherein the temperature setting value in the batching tank 3 is 50°C, the pH setting value is 5, the maximum liquid level setting value is 85% and the minimum liquid level setting value is 75%;

[0119] The liquid from the batching tank 3 is continuously input into the first-stage enzymolysis tank through the first batching pump to finally produce enzymolysis liquid. During the enzymolysis process, the control system 5 is automatically adjusted in real time to control the temperature in the three enzymolysis tanks to 50°C, the pH value to 5 and the liquid level to 75% of the enzymolysis tank.

[0120] In this embodiment, the total residence time of the batching tank and the three enzymolysis tanks is controlled at 96 hours.

[0121] [Comparative Example 1]

[0122] The enzymolysis equipment used in this comparative example includes a belt conveyor, a screw conveyor and an enzymolysis tank which are connected in sequence.

[0123] The specific process of no results is:

[0124] Feeding: First, add water to the enzymolysis tank, then convey the pretreated straw to the enzymolysis tank through a belt conveyor and a screw conveyor, mix and prepare the batching liquid, the dry matter concentration of the pretreated material is controlled at 20%, and the dry matter concentration of the batching is controlled at 10%; then add the enzyme preparation, the enzyme addition ratio range is controlled at 2, and the liquid level is controlled at 70% of the enzymolysis tank. The above process takes 2 hours.

[0125] Reaction: During the enzymolysis process, the pH value in the enzyme tank is controlled at about 4 by adjusting the liquid alkali valve, the temperature in the enzymolysis tank is controlled at 45°C, and the enzymolysis time is controlled at 48 hours.

[0126] Discharging: After the enzymatic hydrolysis is completed, open the discharging pump to discharge all the enzymatic hydrolysis liquid in the enzymatic hydrolysis tank. This process takes 1 hour.

[0127] [Comparative Example 2]

[0128] The equipment used in Comparative Example 2 is the same as that in Comparative Example 1, except that:

[0129] Feeding: First, add water to the enzymolysis tank, then convey the pretreated straw to the enzymolysis tank through a belt conveyor and a screw conveyor, mix and prepare the batching liquid, the dry matter concentration of the pretreated material is controlled at 50%, and the dry matter concentration of the batching is controlled at 30%; then add the enzyme preparation, and the enzyme preparation addition ratio range is controlled at 20. The liquid level is controlled at 75% of the enzymolysis tank. The above process takes 3 hours.

[0130] Reaction: During the enzymatic hydrolysis process, the pH value in the enzyme tank is controlled at about 4 by adjusting the liquid alkali valve, the temperature in the enzymatic hydrolysis batching tank is controlled at 45°C, and the enzymatic hydrolysis time is controlled at 96 hours.

[0131] Discharging: After the enzymatic hydrolysis is completed, open the discharging pump to discharge all the enzymatic hydrolysis liquid in the enzymatic hydrolysis tank. This process takes 1 hour.

[0132] Table 1 Operation process and time comparison of the embodiments and comparative examples

[0133] Example 6 Comparative Example 1 Operation process Continuous operation Intermittent operation requires three steps: feeding, reaction, and discharging Operation time Stay time 48h Feed 2h, reaction 48h, discharge 1h, total time 51h Example 7 Comparative Example 2 Operation process Continuous operation Intermittent operation requires three steps: feeding, reaction, and discharging Operation time Stay time 96h Feed 3h, reaction 96h, discharge 1h, total time required 100h

[0134] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "connected" and "connection" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0135] In the description of the present invention, unless otherwise specified, the terms "upper", "lower", "left", "right", "inside", "outside", etc. indicate directions or positional relationships based on the directions or positional relationships shown in the accompanying drawings. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction. Therefore, they cannot be understood as limitations on the present invention.

[0136] The above technical solution is only one implementation mode of the present invention. For those skilled in the art, it is easy to make various types of improvements or modifications based on the principles disclosed in the present invention, and it is not limited to the technical solution described in the above specific embodiments of the present invention. Therefore, the above description is only preferred and does not have a restrictive meaning.

Claims

1. A multi-stage series continuous enzymatic reaction device, characterized in that: It includes a belt conveyor, a screw conveyor, a batching tank and at least one enzymolysis tank connected in sequence; It also includes a control system for controlling the continuous operation of the batching tank and the enzymolysis tank.

2. The multi-stage series continuous enzymolysis reaction equipment according to claim 1, characterized in that: The control system includes a batching control unit and an enzymatic hydrolysis control unit; The batching control unit includes a calculation module and a feeding control module; The calculation module includes a first calculation module and a second calculation module, wherein the first calculation module is used to calculate the mass flow rate of the upper water according to the mass flow rate of the material entering the batching tank, and the second calculation module is used to calculate the mass flow rate of the enzyme preparation according to the mass flow rate of the material entering the batching tank; The feed control module includes a first feed control module and a second feed control module. The first feed control module is connected to the first calculation module and is used to control the mass flow of water entering the batching tank according to the calculation results of the first calculation module. The second feed control module is connected to the second calculation module and is used to control the mass flow of enzyme preparation entering the batching tank according to the calculation results of the second calculation module.

3. The multi-stage series continuous enzymolysis reaction equipment according to claim 2, characterized in that: The batching control unit also includes a first temperature control module, a first pH control module and a first liquid level control module. The first temperature control module is used to control the temperature in the batching tank to be at a set temperature value; the first pH control module is used to control the pH value in the batching tank to be at a set pH value; the first liquid level control module is used to control the liquid level in the batching tank to be at a set liquid level value.

4. The multi-stage series continuous enzymolysis reaction equipment according to claim 3, characterized in that: The enzymolysis control unit includes a second temperature control module, a second pH control module and a second liquid level control module. The second temperature control module is used to control the temperature in the enzymolysis tank to a set temperature value; the second pH control module is used to control the pH value in the enzymolysis tank to a set pH value; the second liquid level control module is used to control the liquid level in the enzymolysis tank to a set liquid level value.

5. The multi-stage series continuous enzymolysis reaction equipment according to claim 4, characterized in that: The top of the batching tank is connected to a water supply pipeline, and a first mass flow transmitter and a water supply regulating valve are arranged on the water supply pipeline, and the first mass flow transmitter and the water supply regulating valve are both connected to the first feed control module; The top of the batching tank is also connected to a variable frequency batching pump through a pipeline, and the pipeline is also connected to a second mass flow transmitter. The variable frequency batching pump and the second mass flow transmitter are both connected to the second feed control module.

6. The multi-stage series continuous enzymolysis reaction equipment according to claim 5, characterized in that: A first circulating water system is arranged on the outer wall of the batching tank, a water inlet of the first circulating water system is connected to an external hot water supply pipe through a pipe, a first hot water valve is arranged on the pipe connecting the water inlet and the external hot water supply pipe, and the first hot water valve is connected to a first temperature control module; The batching tank is also provided with a first temperature transmitter connected to the first temperature control module.

7. The multi-stage series continuous enzymolysis reaction equipment according to claim 6, characterized in that: The top of the batching tank is also connected to a first liquid alkali pipeline, on which a first liquid alkali valve is provided, and the first liquid alkali valve is connected to a first pH control module; The batching tank is also provided with a first pH meter connected to the first pH control module.

8. The multi-stage series continuous enzymolysis reaction equipment according to claim 7, characterized in that: The bottom of the batching tank is provided with a discharge port, the discharge port is connected to a first batching pump through a pipeline, the first batching pump is connected to a reflux port provided at the top of the batching tank through a first pipeline, the first batching pump is connected to the enzymolysis tank through a second pipeline, and the second pipeline is provided with a first liquid level regulating valve; The batching tank is provided with a first liquid level transmitter, and the first liquid level control module is respectively connected to the first liquid level regulating valve, the first liquid level transmitter and the control switch of the belt conveyor.

9. The multi-stage series continuous enzymolysis reaction equipment according to claim 8, characterized in that: A second circulating water system is provided on the outer wall of the enzymolysis tank, the water inlet of the second circulating water system is connected to an external hot water supply pipe through a pipe, a second hot water regulating valve is provided on the pipe connecting the water inlet and the external hot water supply pipe, and the second hot water regulating valve is connected to the second temperature control module; The enzymolysis tank is also provided with a second temperature transmitter connected to the second temperature control module.

10. The multi-stage series continuous enzymolysis reaction equipment according to claim 9, characterized in that: The top of the enzymolysis tank is also connected to a second liquid alkali pipeline, on which a second liquid alkali valve is provided, and the second liquid alkali valve is connected to a second pH control module; The enzymolysis tank is also provided with a second pH meter connected to the second pH control module.

11. The multi-stage series continuous enzymolysis reaction equipment according to claim 10, characterized in that: The bottom of the enzymolysis tank is provided with a discharge port, the discharge port is connected to the second dosing pump through a pipeline, the second dosing pump is connected to the reflux port provided on the upper part of the enzymolysis tank through the first delivery pipeline, the second dosing pump is connected to the next-level enzymolysis tank or storage tank through the second delivery pipeline, and the second delivery pipeline is provided with a second liquid level regulating valve, and the second liquid level regulating valve is connected to the second liquid level control module; A second liquid level transmitter is provided in the enzymolysis tank, and the second liquid level transmitter is connected to the second liquid level control module.

12. A multi-stage series continuous enzymolysis method, characterized in that: The method is carried out using the multi-stage series continuous enzymolysis reaction equipment according to any one of claims 1 to 11, wherein the method comprises a batching process and an enzymolysis process. (I) The batching process specifically includes: The pre-treated materials are continuously transported to the batching tank through belt conveyors and screw conveyors; The control system calculates the mass flow rate of the feed water and the mass flow rate of the enzyme preparation according to the flow rate of the pre-treated material entering the batching tank, and controls the feed water and the enzyme preparation to enter the batching tank; During the batching process, the control system automatically adjusts the temperature, pH value and liquid level in the batching tank in real time; (II) The enzymatic hydrolysis process specifically includes: The batching liquid in the batching tank is input into the enzymolysis tank through the control system to finally produce enzymolysis liquid. During the enzymolysis process, the control system automatically adjusts the temperature, pH value and liquid level in the enzymolysis tank in real time.

13. The multi-stage series continuous enzymolysis method according to claim 12, characterized in that: The calculation formula for the water mass flow rate is: The enzyme preparation mass flow calculation formula is: Enzyme preparation mass flow rate = pretreatment material mass flow rate × enzyme addition ratio.

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