Pumpkin seed protein peptide with function of delaying muscle attenuation as well as preparation method and application of pumpkin seed protein peptide

By performing specific enzymatic treatment on the crude pumpkin seed protein, pumpkin seed protein peptide was prepared, and the C. elegans model was verified to delay muscle loss and improve exercise ability, which solved the problem of difficult to effectively delay muscle attenuation in the existing technology, and achieved Significant muscle protection and exercise capacity improvement.

CN119307575AInactive Publication Date: 2025-01-14完美(广东)日用品有限公司 +1
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Patent Information

Application Number
CN202411416106.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-10-11
Publication Date
2025-01-14
Estimated Expiration
Not applicable · inactive patent

AI Technical Summary

Technical Problem

The prior art is difficult to effectively delay muscle attenuation, especially through peptide compositions, and there are no relevant reports on pumpkin seed protein peptides in this field, and it is impossible to clarify the effect of delaying muscle attenuation.

Method used

By mixing the crude pumpkin seed protein with water for homogenization, adjusting the pH value and adding alkaline protease, trypsin or flavor protease for enzymatic decomposition, pumpkin seed protein peptide was obtained, and it was verified by the C. nematode model to delay muscle loss and improve The efficacy of muscle motor ability.

Benefits of technology

The preparation of pumpkin seed protein peptide is achieved, which significantly delays muscle loss, protects muscle tissue, and improves muscle motility, thereby effectively delaying muscle attenuation.

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Abstract

The invention relates to pumpkin seed protein peptide with a sarcopenia delaying effect and a preparation method and application thereof, and belongs to the technical field of biology. The invention provides a preparation method of pumpkin seed protein peptide with a sarcopenia delaying effect, which comprises the following steps: S1, mixing pumpkin seed crude protein with water, and homogenizing to obtain homogenized liquid; s2, adjusting the pH value of the homogenate obtained in the step S1 to 7.5-10.0, adding protease, performing enzymolysis at 50-60 DEG C for 2-10 hours, and performing enzyme deactivation to obtain enzymatic hydrolysate; and S3, centrifuging the enzymatic hydrolysate obtained in the step S2, wherein the obtained supernatant is the pumpkin seed protein peptide. Crude pumpkin seed protein is subjected to enzymolysis under specific conditions to obtain the pumpkin seed protein peptide, and it is proved through a caenorhabditis elegans model that the pumpkin seed protein peptide can delay muscle loss, protect muscular tissue and improve the athletic ability of muscles, so that the effect of delaying muscle attenuation is achieved.
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Citation Information

Patent Citations

  • Pumpkin seed peptide powder as well as a preparation method and application thereof

    CN111019990A

  • Composition comprising a combination of vegetable proteins and peptides

    EP3763228A1

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