Gelling composition of carrageenan as well as preparation method and application thereof

Abstract

The invention discloses a gelation composition of carrageenan as well as a preparation method and application thereof, and the preparation method comprises the following steps: S1, mixing yeast beta-glucan, carrageenan and deionized water, and carrying out high-temperature and high-pressure homogenization reaction to obtain a mixture A; s2, cooling the mixture A to room temperature; adding tea seed oil, radix rehmanniae, allantoin and dipotassium glycyrrhizinate, and performing low-temperature and high-pressure homogenization to obtain a mixture B; s3, adjusting the pH value of the mixture B to be 5-6 by adopting a pH regulator; and adding a preservative to obtain the gelling composition. According to the preparation method of the gelling composition of the carrageenan, the gelling composition of the yeast beta-glucan and the carrageenan is formed through a high-temperature and high-pressure homogenization reaction, and then all efficacy components are uniformly dispersed in the gelling composition through low-temperature and high-pressure homogenization, so that the dispersion uniformity of the efficacy components in the gelling composition is improved; meanwhile, an effective protective layer can be formed on the skin surface in the form of the gel composition, so that the effect of each functional component is lasting and efficient.

Description

Technical Field

[0001] The invention relates to the technical field of gelling compositions, and in particular to a gelling composition of carrageenan and a preparation method and application thereof. Background Art

[0002] Burns often occur frequently in daily life and work. Severe burns require medical surgery, while minor burns can be relieved by ointments or Chinese medicine compositions. In the prior art, burn ointments are all special components formed by Chinese medicine compositions.

[0003] CN117503860A discloses a scald plaster, which is composed of a mercurochrome composition and a medicine surface, and the mercurochrome composition and the medicine surface are used separately, wherein the mercurochrome composition comprises 3-9 parts of sulfonamide crystals, 20-40 parts of mercurochrome and 4-10 parts of zinc oxide in parts by weight, and the medicine surface comprises 4-10 parts of mugwort, 3-9 parts of cactus, 10-20 parts of aloe peel, 6-12 parts of white worm wax, 5-15 parts of beeswax, 8-20 parts of ascidian, 4-10 parts of drossop, 4-10 parts of borneol, 8-20 parts of honeysuckle, 4-10 parts of peony bark, and 3-9 parts of dandelion in parts by weight. Among them, the mercurochrome composition has antibacterial, anti-inflammatory and anti-infection effects; Artemisia argyi has the effects of clearing away heat and detoxifying, promoting blood circulation and removing blood stasis, and clearing rashes and removing spots; cactus has antibacterial, anti-inflammatory, anti-irritation and repairing effects, and has good stability and skin compatibility; aloe peel has the effects of promoting blood circulation, reducing swelling, promoting tissue regeneration and relieving pain; ascidian has the effects of reconciling the exterior and interior, relieving liver depression and raising yang energy; dragon grass can increase granulation, tissue structure and epidermal cell regeneration, repair skin appendages and has a strong wound healing effect; honeysuckle reduces swelling and relieves pain, and is used for mild burns caused by water and fire to relieve skin swelling and pain; peony bark has an inhibitory effect on a variety of bacteria and viruses, and effectively prevents wound infection; dandelion has the effects of clearing heat and relieving pain, promoting tissue regeneration, promoting blood circulation and unblocking meridians, and relieving swelling and pain in sores and ulcers. However, in the preparation process of the traditional Chinese medicine composition, various mixed powders are added to white insect wax and beeswax, and after sufficient mixing, they are added to a stirring kettle and heated and stirred to obtain a finished plaster. This stirring method can only physically combine the various compositions together. During the plaster packaging process, the various compositions may be unevenly distributed, thereby affecting the medicinal properties of the plaster.

[0004] The existing preparation method for burn ointment cannot make various effective ingredients evenly and effectively distributed, and cannot maximize the efficacy of the burn ointment. Summary of the invention

[0005] The object of the present invention is to provide a method for preparing a gel composition of carrageenan, wherein a gel composition of yeast β-glucan and carrageenan is formed by a high-temperature and high-pressure homogenization reaction, and then various functional ingredients are uniformly dispersed in the gel composition by low-temperature and high-pressure homogenization, thereby improving the dispersion uniformity of the functional ingredients in the gel composition. At the same time, the form of the gel composition can form an effective protective layer on the skin surface, thereby making the effects of various functional ingredients lasting and efficient.

[0006] To achieve the above object, a method for preparing a carrageenan gel composition is provided, comprising:

[0007] S1: mixing yeast β-glucan, carrageenan and deionized water and subjecting them to high temperature and high pressure homogenization reaction to obtain a mixture A;

[0008] S2: Cooling the mixture A to room temperature; adding tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizinate to perform low temperature and high pressure homogenization to obtain a mixture B;

[0009] S3: adjusting the pH value of the mixture B to 5-6 with a pH adjuster; and adding a preservative to obtain a gelled composition.

[0010] Furthermore, the homogenization pressure of the high temperature and high pressure homogenization is 8-10 MPa, and the homogenization temperature is 50-70°C.

[0011] Furthermore, the pressure of the low-temperature and high-pressure homogenization is 6-8 MPa, and the homogenization temperature is 20-25°C.

[0012] Furthermore, the mass ratio of the camellia oil, rehmannia root, allantoin and dipotassium glycyrrhizinate is 1:1:2:2.

[0013] Furthermore, the mass ratio of the sum of the masses of the camellia oil, rehmannia root, allantoin and dipotassium glycyrrhizinate to the mixture A is 1:10.

[0014] Furthermore, the mass ratio of the yeast β-glucan to carrageenan is 5:1.

[0015] Furthermore, the preservative includes at least one of methyl parahydroxybenzoate, ethyl parahydroxybenzoate and butyl parahydroxybenzoate.

[0016] Furthermore, the pH adjuster is sodium citrate.

[0017] A carrageenan gelling composition is prepared based on the preparation method as described above.

[0018] The carrageenan gel composition as described above is used for preparing burn ointment.

[0019] Beneficial effects of the present invention:

[0020] (1) The present invention forms a gelled composition from yeast β-glucan and carrageenan through a high temperature and high pressure homogenization reaction, thereby forming the main scaffold component of the composition of the present application. Yeast β-glucan does not affect the gelling effect of carrageenan, and the two undergo a gelling reaction during the high temperature and high pressure homogenization process to form an interlaced network structure. The finally formed network gelled scaffold can serve as the main scaffold for carrying its effective ingredients, and can also serve as an outer protective film of the skin to cover and protect the effective ingredients and the burn site during use.

[0021] (2) The present invention further adds tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizinate as effective ingredients, wherein tea seed oil and rehmannia root can play a role in healing burns, and allantoin and dipotassium glycyrrhizinate have good anti-inflammatory effects. Tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizinate are uniformly dispersed in a mesh gel scaffold under low temperature and high pressure homogenization conditions, so that the effective ingredients can be uniformly dispersed in the carrier. At the same time, the low temperature environment can also ensure that the biological activity and efficacy of tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizinate are not destroyed, thereby ensuring that they can fully exert their efficacy during use. DETAILED DESCRIPTION

[0022] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. Without departing from the spirit and essence of the present invention, modifications or substitutions made to the methods, steps or conditions of the present invention are within the scope of protection of the present invention. Unless otherwise specified, the experimental materials, reagents, instruments, etc. used in the examples of this application are all commercially available; unless otherwise specified, all technical means in the examples of the present invention are conventional means well known to those skilled in the art.

[0023] The present application provides a method for preparing a carrageenan gel composition, comprising:

[0024] S1: Yeast β-glucan, carrageenan and deionized water are mixed and subjected to high temperature and high pressure homogenization reaction to obtain a mixture A; wherein the homogenization pressure of the high temperature and high pressure homogenization is 8-10 MPa, and the homogenization temperature is 50-70° C. The mass ratio of the yeast β-glucan to carrageenan is 5:1.

[0025] S2: Cool the mixture A to room temperature; add tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizinate for low temperature and high pressure homogenization to obtain a mixture B. The pressure of the low temperature and high pressure homogenization is 6-8 MPa, and the homogenization temperature is 20-25°C. The mass ratio of the tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizinate is 1:1:2:2. The mass ratio of the sum of the mass of the tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizinate to the mass of the mixture A is 1:10.

[0026] S3: Using a pH adjuster to adjust the pH value of the mixture B to 5-6; and adding a preservative to obtain a gel composition. The preservative includes at least one of methyl parahydroxybenzoate, ethyl parahydroxybenzoate, and butyl parahydroxybenzoate. The pH adjuster is sodium citrate.

[0027] The present invention forms a gelled composition of yeast β-glucan and carrageenan through a high temperature and high pressure homogenization reaction, forming the main scaffold component of the composition of the present application, wherein yeast β-glucan will not affect the gelling effect of carrageenan, and the two undergo a gelling reaction during the high temperature and high pressure homogenization process to form an interlaced mesh structure, and the mesh gelled scaffold finally formed can be used as the main scaffold for carrying its effective components, and can also be used as an outer protective film of the skin to cover and protect the effective components and the scald position during use. The homogenization pressure of the high temperature and high pressure homogenization is 8-10MPa, and the homogenization temperature is 50-70°C. Under this condition, yeast β-glucan and carrageenan can undergo a gelling reaction well to form a mesh gelled scaffold.

[0028] The present invention also adds tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizinate as effective ingredients, wherein tea seed oil and rehmannia root can play a role in healing burns, allantoin and dipotassium glycyrrhizinate have good anti-inflammatory effects, tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizinate are uniformly dispersed in a mesh gelling scaffold under low temperature and high pressure homogenization conditions, so that the effective ingredients can be uniformly dispersed in the carrier, and at the same time, the low temperature environment can also ensure that the biological activity and efficacy of tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizinate are not destroyed, ensuring that they can fully exert their efficacy during use. The pressure of low temperature and high pressure homogenization is 6-8MPa, and the homogenization temperature is 20-25°C. Under this condition, the biological activity and efficacy of tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizinate will not be destroyed, and under high pressure homogenization, they can be uniformly dispersed in the mesh gelling scaffold.

[0029] The gel composition prepared in the present application can be used as a burn ointment. During use as a burn ointment, the mesh gel scaffold can cover the burned skin, so that the tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizinate embedded in the mesh gel scaffold can fully exert their effects, relieve burns, and enhance the skin's repair ability.

[0030] Example 1

[0031] The present embodiment provides a method for preparing a carrageenan gel composition, comprising:

[0032] S1: Yeast β-glucan, carrageenan and deionized water are mixed and subjected to high temperature and high pressure homogenization reaction to obtain a mixture A; wherein the homogenization pressure of the high temperature and high pressure homogenization is 8 MPa, and the homogenization temperature is 50° C.; the mass ratio of yeast β-glucan to carrageenan is 5:1.

[0033] S2: Cool the mixture A to room temperature; add tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizinate for low temperature and high pressure homogenization to obtain a mixture B. The pressure of the low temperature and high pressure homogenization is 6 MPa, and the homogenization temperature is 20°C. The mass ratio of the tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizinate is 1:1:2:2; the mass ratio of the sum of the mass of the tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizinate to the mixture A is 1:10.

[0034] S3: adjusting the pH value of the mixture B to 5-6 with sodium citrate, and adding methyl paraben to obtain a gel composition. The gel composition is used as a burn ointment.

[0035] Example 2

[0036] The present embodiment provides a method for preparing a carrageenan gel composition, comprising:

[0037] S1: Yeast β-glucan, carrageenan and deionized water are mixed and subjected to high temperature and high pressure homogenization reaction to obtain a mixture A; wherein the homogenization pressure of the high temperature and high pressure homogenization is 10 MPa, and the homogenization temperature is 70° C.; the mass ratio of yeast β-glucan to carrageenan is 5:1.

[0038] S2: Cool the mixture A to room temperature; add tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizinate for low temperature and high pressure homogenization to obtain a mixture B. The pressure of the low temperature and high pressure homogenization is 8 MPa, and the homogenization temperature is 25°C. The mass ratio of the tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizinate is 1:1:2:2; the mass ratio of the sum of the mass of the tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizinate to the mixture A is 1:10.

[0039] S3: adjusting the pH value of the mixture B to 5-6 with sodium citrate, and adding methyl paraben to obtain a gel composition. The gel composition is used as a burn ointment.

[0040] Example 3

[0041] The present embodiment provides a method for preparing a carrageenan gel composition, comprising:

[0042] S1: Yeast β-glucan, carrageenan and deionized water are mixed and subjected to high temperature and high pressure homogenization reaction to obtain a mixture A; wherein the homogenization pressure of the high temperature and high pressure homogenization is 9 MPa, and the homogenization temperature is 60° C.; the mass ratio of yeast β-glucan to carrageenan is 5:1.

[0043] S2: Cool the mixture A to room temperature; add tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizinate for low temperature and high pressure homogenization to obtain a mixture B. The pressure of the low temperature and high pressure homogenization is 7 MPa, and the homogenization temperature is 22°C. The mass ratio of the tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizinate is 1:1:2:2; the mass ratio of the sum of the mass of the tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizinate to the mass of the mixture A is 1:10.

[0044] S3: adjusting the pH value of the mixture B to 5-6 with sodium citrate, and adding methyl paraben to obtain a gel composition. The gel composition is used as a burn ointment.

[0045] Comparative Example 1

[0046] The present embodiment provides a method for preparing a carrageenan gel composition, comprising:

[0047] S1: Yeast β-glucan, carrageenan, deionized water, tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizinate are mixed and subjected to high temperature and high pressure homogenization reaction, wherein the homogenization pressure of the high temperature and high pressure homogenization is 9 MPa and the homogenization temperature is 60°C; the mass ratio of yeast β-glucan to carrageenan is 5:1. The mass ratio of tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizinate is 1:1:2:2; the mass ratio of the sum of the mass of tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizinate to the mass of mixture A is 1:10.

[0048] S2: adjusting the pH value of the mixture B to 5-6 with sodium citrate, and adding methyl paraben to obtain a gelled composition. The gelled composition is used as a burn ointment.

[0049] Comparative Example 2

[0050] The present embodiment provides a method for preparing a carrageenan gel composition, comprising:

[0051] S1: Yeast β-glucan, carrageenan, deionized water, tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizinate are mixed and subjected to low temperature and high pressure homogenization reaction, wherein the homogenization pressure of the low temperature and high pressure homogenization is 7 MPa and the homogenization temperature is 22°C; the mass ratio of yeast β-glucan to carrageenan is 5:1. The mass ratio of the tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizinate is 1:1:2:2; the mass ratio of the sum of the mass of the tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizinate to the mass of the mixture A is 1:10.

[0052] S2: adjusting the pH value of the mixture B to 5-6 with sodium citrate, and adding methyl paraben to obtain a gelled composition. The gelled composition is used as a burn ointment.

[0053] Comparative Example 3

[0054] The present embodiment provides a method for preparing a carrageenan gel composition, comprising:

[0055] S1: Yeast β-glucan, carrageenan and deionized water are mixed and subjected to high temperature and high pressure homogenization reaction to obtain a mixture A; wherein the homogenization pressure of the high temperature and high pressure homogenization is 9 MPa, and the homogenization temperature is 60° C.; the mass ratio of yeast β-glucan to carrageenan is 5:1.

[0056] S2: Cooling the mixture A to room temperature; adding tea seed oil and Rehmannia root for low temperature and high pressure homogenization to obtain a mixture B. The pressure of the low temperature and high pressure homogenization is 7 MPa, and the homogenization temperature is 22°C. The mass ratio of the tea seed oil to Rehmannia root is 1:1; the mass ratio of the sum of the mass of the tea seed oil and Rehmannia root to the mixture A is 1:10.

[0057] S3: adjusting the pH value of the mixture B to 5-6 with sodium citrate, and adding methyl paraben to obtain a gel composition. The gel composition is used as a burn ointment.

[0058] Comparative Example 4

[0059] The present embodiment provides a method for preparing a carrageenan gel composition, comprising:

[0060] S1: Yeast β-glucan, carrageenan and deionized water are mixed and subjected to high temperature and high pressure homogenization reaction to obtain a mixture A; wherein the homogenization pressure of the high temperature and high pressure homogenization is 9 MPa, and the homogenization temperature is 60° C.; the mass ratio of yeast β-glucan to carrageenan is 5:1.

[0061] S2: Cooling the mixture A to room temperature; adding allantoin and dipotassium glycyrrhizinate for low temperature and high pressure homogenization to obtain a mixture B. The pressure of the low temperature and high pressure homogenization is 7 MPa, and the homogenization temperature is 22°C. The mass ratio of the allantoin and dipotassium glycyrrhizinate is 1:1; the mass ratio of the sum of the mass of the allantoin and dipotassium glycyrrhizinate to the mass of the mixture A is 1:10.

[0062] S3: adjusting the pH value of the mixture B to 5-6 with sodium citrate, and adding methyl paraben to obtain a gel composition. The gel composition is used as a burn ointment.

[0063] Experimental example

[0064] 1. Establishment of mouse scald model

[0065] 24 hours before the experiment, apply an appropriate amount of 5% sodium sulfide liquid to the back of the mouse for hair removal. The hairless area on the back of the mouse is about 2×2 cm. When establishing the scald model, a 50g weight is placed in boiling water for 10 minutes, then quickly taken out and tightly adhered to the smooth hairless skin of the mouse for 8 seconds to complete the deep II degree scald model. The blank control group was not treated.

[0066] 2. Skin healing rate

[0067] 50 mice were divided into 5 groups and treated with the burn ointments of Examples 1 to 3 and Comparative Examples 1 to 4, respectively. 5% sodium sulfide was applied to the back of the mice to remove hair, with an area of ​​2×2 cm. The back skin of the mice was tightly placed on a weight in boiling distilled water, and the time was measured with a stopwatch. The mice in each group were scalded for 8 seconds, and the scald model was completed.

[0068] After the mouse scald model was completed, 0.1 g of scald ointment was applied to the wound skin once a day, and the wound healing rate was calculated. The results are shown in Table 1.

[0069] On the 14th day after the model was completed, the sulfuric acid paper marking method was used to replace the area with the mass of the marked area, and the average wound healing rate was calculated. The specific calculation formula is as follows:

[0070] Wound healing rate = (original scald area - unhealed area) / original scald area × 100%

[0071] Criteria for judging wound healing: the scab on the wound falls off, new epidermis is generated, and tiny villi appear.

[0072] Table 1 Mouse skin healing rate in each embodiment and comparative example

[0073]

[0074] It can be seen that: under the same components, only high temperature and high pressure homogenization or low temperature and high pressure homogenization cannot fully exert the effect of the effective ingredients, and the mouse skin healing rate is greater than that of the blank control group, but is much lower than the mouse skin healing rate corresponding to the scald ointment in Examples 1 to 3.

[0075] Under the same process conditions, compared with the comparative examples containing only camellia oil and rehmannia, or only allantoin and dipotassium glycyrrhizinate, the effective ingredients of Examples 1 to 3 of the present application, which simultaneously contain camellia oil, rehmannia, allantoin and dipotassium glycyrrhizinate, can significantly improve the healing rate of mouse skin.

[0076] It can be understood that the above embodiments only express the preferred implementation modes of the present invention, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the patent scope of the present invention. It should be pointed out that, for ordinary technicians in this field, the above technical features can be freely combined without departing from the concept of the present invention, and several deformations and improvements can be made, which all belong to the protection scope of the present invention. Therefore, all equivalent changes and modifications made to the scope of the claims of the present invention should belong to the coverage of the claims of the present invention.

Claims

1. A method for preparing a carrageenan gel composition, characterized in that: include: S1: mixing yeast β-glucan, carrageenan and deionized water and subjecting them to high temperature and high pressure homogenization reaction to obtain a mixture A; S2: Cooling the mixture A to room temperature; adding tea seed oil, rehmannia root, allantoin, and dipotassium glycyrrhizinate to perform low temperature and high pressure homogenization to obtain a mixture B; S3: adjusting the pH value of the mixture B to 5-6 with a pH adjuster; and adding a preservative to obtain a gelled composition.

2. The method for preparing a carrageenan gel composition according to claim 1, characterized in that: The homogenization pressure of the high temperature and high pressure homogenization is 8-10 MPa, and the homogenization temperature is 50-70°C.

3. The method for preparing a carrageenan gel composition according to claim 1, characterized in that: The pressure of the low temperature and high pressure homogenization is 6-8 MPa, and the homogenization temperature is 20-25°C.

4. The method for preparing a carrageenan gel composition according to claim 1, characterized in that: The mass ratio of the tea seed oil, rehmannia root, allantoin and dipotassium glycyrrhizinate is 1:1:2:

2.

5. The method for preparing a carrageenan gel composition according to claim 1, characterized in that: The mass ratio of the sum of the masses of the camellia oil, rehmannia root, allantoin and dipotassium glycyrrhizinate to the mixture A is 1:

10.

6. The method for preparing a carrageenan gel composition according to claim 1, characterized in that: The mass ratio of the yeast β-glucan to carrageenan is 5:

1.

7. The method for preparing a carrageenan gel composition according to claim 1, characterized in that: The preservative includes at least one of methyl parahydroxybenzoate, ethyl parahydroxybenzoate and butyl parahydroxybenzoate.

8. The method for preparing a carrageenan gel composition according to claim 1, characterized in that: The pH adjuster is sodium citrate.

9. A carrageenan gelling composition, characterized in that: It is prepared based on the preparation method according to any one of claims 1 to 5.

10. The use of the carrageenan gel composition according to claim 7, characterized in that: Used to prepare burn ointment.

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