Hair extracting solution for drug detection and preparation method thereof

Abstract

The invention provides a hair extracting solution for drug detection and a preparation method thereof, and belongs to the technical field of extracting solutions for drug hair detection, and the extracting solution comprises the following components: trihydroxymethyl aminomethane, boric acid, hydrogen peroxide, lauryl sodium sulfate and sodium chloride. The preparation method comprises the following steps: preparation work, preparation of a basic solution, addition of active components, addition of methanol, constant volume and mixing, inspection and storage. The method has the characteristics of efficient detection, low cost production, easy operation, good stability and the like.

Description

Technical Field

[0001] The present invention relates to the technical field of hair extract for drug detection, and in particular to a hair extract for drug detection and a preparation method thereof. Background Art

[0002] At present, hair as a biological sample is increasingly valued in drug detection because it can reflect drug use over a long period of time. However, traditional hair drug detection methods have problems such as complex operation, long detection time, and low sensitivity. Existing extracts mostly rely on high-cost proteases or complex processing steps, which not only increase the detection cost but also extend the detection cycle. Therefore, it is particularly important to develop a hair extract for drugs that is low in cost, easy to operate, and has high detection efficiency. To this end, the present invention provides a hair extract for drug detection and a preparation method thereof. Summary of the invention

[0003] The purpose of the present invention is to provide a hair extract for drug detection and a preparation method thereof.

[0004] To solve the above technical problems, the purpose of the present invention is achieved as follows:

[0005] A hair extract for drug detection comprises the following components by mass percentage: 1%-5% of tris(hydroxymethyl)aminomethane, 0.5%-3% of boric acid, 1%-4% of hydrogen peroxide, 0.1%-1% of sodium dodecyl sulfate, and 5%-15% of sodium chloride.

[0006] On the basis of the above scheme and as a preferred scheme of the above scheme, the hair extract for drug detection includes the following components by mass percentage: 2%-3% of trishydroxymethylaminomethane, 1%-2% of boric acid, 2%-3% of hydrogen peroxide, 0.5%-0.7% of sodium dodecyl sulfate, and 8%-12% of sodium chloride.

[0007] On the basis of the above scheme and as a preferred scheme of the above scheme, the hair extract for drug detection also includes dithiothreitol; the mass percentage of the dithiothreitol is 0.01%-0.1%.

[0008] On the basis of the above scheme and as a preferred scheme of the above scheme, the hair extract for drug detection also includes methanol; the mass percentage of the methanol is 5-20%.

[0009] On the basis of the above scheme and as a preferred scheme of the above scheme, the hair extract for drug detection also includes a stabilizer and a preservative; the mass percentage of the stabilizer is 1%-5%; the mass percentage of the preservative is 0.01%-0.2%.

[0010] On the basis of the above scheme and as a preferred scheme of the above scheme, the stabilizer is ethylenediaminetetraacetic acid or citric acid.

[0011] On the basis of the above scheme and as a preferred scheme of the above scheme, the preservative is sodium azide or sodium formate.

[0012] A method for preparing a hair extract for drug detection comprises the following steps:

[0013] S1. Preparation: including material preparation and equipment preparation;

[0014] The material preparation is to collect and prepare all the required raw materials, including tris(hydroxymethyl)aminomethane, boric acid, hydrogen peroxide, sodium lauryl sulfate, sodium chloride, dithiothreitol, methanol, and preservatives and stabilizers planned to be added;

[0015] The equipment preparation includes preparing a weighing device, a stirring device, a pH measuring device, a solid raw material container, a liquid raw material container, a pipetting device and a liquid measuring device;

[0016] S2, preparing a basic solution, comprising the following steps:

[0017] Weighing: Use a weighing device to accurately weigh a certain amount of solid raw materials such as tris(hydroxymethyl)aminomethane, boric acid and sodium chloride, and place them in a solid raw material container;

[0018] Dissolution: Add an appropriate amount of distilled water into the solid raw material container and stir thoroughly using a stirring device until all the solid raw materials are completely dissolved.

[0019] Adjusting pH: Use a pH measuring device to detect the pH value of the base solution and add an appropriate amount of acid or alkaline solution to adjust it as needed to ensure that the pH value is within the predetermined range;

[0020] S3, adding active ingredients, comprising the following steps:

[0021] Add hydrogen peroxide: slowly add a predetermined amount of hydrogen peroxide to the base solution while stirring, and control the adding speed to avoid violent reaction;

[0022] Add sodium lauryl sulfate: Continue stirring and slowly add the predetermined amount of sodium lauryl sulfate; the addition of sodium lauryl sulfate will increase the viscosity and foam volume of the solution, so keep stirring to ensure it is evenly dispersed;

[0023] Add dithiothreitol: first mix dithiothreitol with distilled water to dissolve it, then add the solvent to the main solution; keep stirring during the addition process;

[0024] Add preservatives and stabilizers; mix and dissolve the preservatives and stabilizers with distilled water, and then add the dissolved solvent to the solution; keep stirring during the addition process;

[0025] S4, adding methanol. Slowly pour a predetermined amount of methanol into the solution while stirring. Since methanol is miscible with water, local heat release may occur when it is added. Therefore, the addition speed needs to be controlled and stirring needs to be continued to dissipate heat.

[0026] S5, volume setting and mixing;

[0027] Make up to volume: Use distilled water to adjust the solution volume to the desired value and continue stirring to ensure that all ingredients are evenly mixed;

[0028] Mix well: Place the solution on a stirring device and continue stirring for a while to ensure that all ingredients are fully dissolved and mixed well;

[0029] S6. Inspection and storage:

[0030] Inspection: Conduct necessary inspections on the prepared extract, such as pH re-inspection, appearance inspection, etc., to ensure that it meets the quality requirements;

[0031] Storage: Transfer the extract to a clean glass or plastic bottle and seal it in a cool, dry, dark place away from fire and heat sources.

[0032] The beneficial effects of the present invention are:

[0033] 1. Improve detection efficiency: Drug hair extracts can achieve rapid extraction of drug components in hair by optimizing the formula and preparation process. For example, some extracts can complete the extraction process within a few minutes to tens of minutes, greatly shortening the detection time. There is no need for complex sample pre-treatment steps, such as grinding, cleaning, and cracking, which simplifies the operation process and reduces the technical requirements for operators.

[0034] 2. Enhanced detection sensitivity: The active ingredients in the extract can efficiently release the drug components in the hair and react with the detection reagents, thereby improving the sensitivity of the detection. It is suitable for detecting extremely low concentrations of drug residues, providing a more reliable scientific basis for forensic identification.

[0035] 3. Maintaining the stability of the test: During the preparation process, the extract is added with stabilizers and other ingredients to ensure its stability during storage and use. It is not easily affected by environmental factors (such as temperature, humidity, etc.), ensuring the accuracy and repeatability of the test results.

[0036] 4. Expand the scope of application: Drug hair extract is not only suitable for the detection of common drugs such as methamphetamine, but also can be adjusted according to the needs to detect other types of drugs or drug residues. It has broad application prospects in the fields of forensic identification, laboratory capacity certification, scientific research and development.

[0037] 5. Reduce testing costs: Compared with traditional testing methods, using hair extracts for drug testing can significantly reduce testing costs. No expensive equipment and complicated operating procedures are required, making testing more economical. DETAILED DESCRIPTION

[0038] The present invention is further described below with regard to specific embodiments.

[0039] Embodiment 1:

[0040] A hair extract for drug detection comprises the following components by mass percentage: 1%-5% of tris(hydroxymethyl)aminomethane, 0.5%-3% of boric acid, 1%-4% of hydrogen peroxide, 0.1%-1% of sodium dodecyl sulfate, 5%-15% of sodium chloride, 0.01%-0.1% of dithiothreitol, 5%-20% of methanol, 1%-5% of a stabilizer and 0.01%-0.2% of a preservative.

[0041] Wherein, the stabilizer is ethylenediaminetetraacetic acid or citric acid; the preservative is sodium azide or sodium formate.

[0042] Tris (hydroxymethylaminomethane): As a buffer, it adjusts the pH value of the extract to ensure stability during the extraction process. Boric acid: It enhances the permeability of the extract and helps release the drug components from the hair. Hydrogen peroxide: As an oxidant, it promotes the interaction between the drug components and the extract and improves the extraction efficiency. Sodium dodecyl sulfate (SDS): As a surfactant, it increases the wettability of the extract on the hair surface and helps dissolve and extract the drug components. Sodium chloride: It adjusts the ionic strength of the extract to further optimize the extraction effect.

[0043] Dithiothreitol (DTT): Function: Dithiothreitol is a strong reducing agent that is primarily used to protect biomolecules from oxidative damage during the extraction process. In the context of drug hair extraction, DTT is able to maintain the integrity of trace proteins, enzymes, or other bioactive substances that may be present in the hair, which may indirectly affect the release or stability of drug components. By preventing the occurrence of oxidation reactions, DTT helps ensure the extraction efficiency of the extract and the accuracy of the test results. Mechanism of action: DTT can break disulfide bonds, thereby preventing the aggregation and denaturation of proteins or other sulfur-containing biomolecules. This protective effect is essential to maintaining the mildness and biocompatibility of the extraction environment.

[0044] Methanol: Function: Methanol, as an organic solvent, mainly plays the role of enhancing the solubility and promoting the release of drug components in the extract of the present invention. The drug components in the hair are often tightly bound to the hair matrix and are difficult to be directly released into the aqueous solution. The addition of methanol can destroy this binding, making the drug components easier to be dissolved and extracted by the extract. Mechanism of action: Methanol has good lipophilicity and permeability, can penetrate into the hair, interact with the drug components, reduce their binding energy, and thus promote their release from the hair and dissolution in the extract. In addition, methanol can also form a co-solvent system with water, further improving the solubility and extraction efficiency of the extract.

[0045] Preservatives: 1. Sodium azide (NaN): Function: Sodium azide is a commonly used preservative that can effectively inhibit the growth of microorganisms such as bacteria and molds, thereby extending the shelf life of the extract. In drug hair extracts, the addition of sodium azide can prevent the extract from being contaminated during storage and ensure the accuracy of the test results. Mechanism of action: Sodium azide achieves the purpose of preservation by destroying the cell structure of microorganisms or interfering with their metabolic process. It can quickly inhibit the growth and reproduction of microorganisms at low concentrations and is relatively non-toxic or less toxic to the human body (but caution is required when handling it, as high concentrations or improper handling may produce toxic gases). 2. Sodium Benzoate: Function: Sodium Benzoate is another common preservative that is widely used in food and cosmetics. In drug hair extracts, it can also inhibit the growth of microorganisms and ensure the stability and safety of the extract. Mechanism of action: Sodium Benzoate achieves the effect of preservation by inhibiting the activity of enzymes in microorganisms to block their metabolic process. It is relatively safe to the human body, but excessive intake may have certain effects on health.

[0046] Stabilizers: 1. Ethylenediaminetetraacetic acid (EDTA): Function: Ethylenediaminetetraacetic acid is an important complexing agent and stabilizer. It is mainly used to prevent the interference of metal ions in drug hair extracts. Metal ions (such as iron, copper, etc.) may react with drug components and affect the accuracy of the test results. EDTA can form stable complexes with these metal ions, thereby removing them from the extract or reducing their interference. Mechanism of action: EDTA has multiple coordinating atoms and can form a stable six-membered ring structure with metal ions, thereby firmly "locking" them in the complex. This complexation not only reduces the activity of metal ions, but also prevents them from reacting unnecessarily with drug components. 2. Citric Acid: Function: Although citric acid is more commonly used in the food industry as an acidulant and preservative, it can also be used as a stabilizer under certain specific conditions. In drug hair extracts, citric acid may enhance the stability of the extract by adjusting the pH value or synergizing with other ingredients. Mechanism of action: Citric acid has multiple carboxyl groups that can chelate with metal ions in water to form stable complexes. In addition, it can also adjust the pH value of the extract to keep it within an appropriate range, thereby avoiding certain chemical reactions or accelerating the release of drug components. However, it should be noted that the specific role of citric acid in drug hair extracts may vary depending on the formulation and experimental conditions.

[0047] A method for preparing a hair extract for drug detection comprises the following steps:

[0048] S1. Preparation: including material preparation and equipment preparation;

[0049] The material preparation is to collect and prepare all the required raw materials, including tris(hydroxymethyl)aminomethane, boric acid, hydrogen peroxide, sodium lauryl sulfate, sodium chloride, dithiothreitol, methanol, and preservatives and stabilizers planned to be added;

[0050] The equipment preparation includes preparing a weighing device, a stirring device, a pH measuring device, a solid raw material container, a liquid raw material container, a pipetting device and a liquid measuring device;

[0051] S2, preparing a basic solution, comprising the following steps:

[0052] Weighing: Use a weighing device to accurately weigh a certain amount of solid raw materials such as tris(hydroxymethyl)aminomethane, boric acid and sodium chloride, and place them in a solid raw material container;

[0053] Dissolution: Add an appropriate amount of distilled water into the solid raw material container and stir thoroughly using a stirring device until all the solid raw materials are completely dissolved.

[0054] Adjusting pH value: using a pH measuring device to detect the pH value of the base solution, and adding an appropriate amount of acid or alkali solution as needed to adjust the pH value to ensure that the pH value is within a predetermined range; the acid or alkali solution is measured by a liquid measuring device to measure the required amount;

[0055] S3, adding active ingredients, comprising the following steps:

[0056] Add hydrogen peroxide: slowly add a predetermined amount of hydrogen peroxide to the base solution while stirring, and control the adding speed to avoid violent reaction;

[0057] Add sodium lauryl sulfate: Continue stirring and slowly add the predetermined amount of sodium lauryl sulfate; the addition of sodium lauryl sulfate will increase the viscosity and foam volume of the solution, so keep stirring to ensure it is evenly dispersed;

[0058] Add dithiothreitol: first mix dithiothreitol with distilled water to dissolve it, then add the solvent to the main solution; keep stirring during the addition process;

[0059] Add preservatives and stabilizers; mix and dissolve the preservatives and stabilizers with distilled water, and then add the dissolved solvent to the solution; keep stirring during the addition process;

[0060] S4, adding methanol. Slowly pour a predetermined amount of methanol into the solution while stirring. Since methanol is miscible with water, local heat release may occur when it is added. Therefore, the addition speed needs to be controlled and stirring needs to be continued to dissipate heat.

[0061] S5, volume setting and mixing;

[0062] Make up to volume: Use distilled water to adjust the solution volume to the desired value and continue stirring to ensure that all ingredients are evenly mixed;

[0063] Mix well: Place the solution on a stirring device and continue stirring for a while to ensure that all ingredients are fully dissolved and mixed well;

[0064] S6. Inspection and storage:

[0065] Inspection: Conduct necessary inspections on the prepared extract, such as pH re-inspection, appearance inspection, etc., to ensure that it meets the quality requirements;

[0066] Storage: Transfer the extract to a clean glass or plastic bottle and seal it. The storage environment should be cool, dry, away from light, and away from fire and heat sources.

[0067] Embodiment 2:

[0068] A hair extract for drug detection comprises the following components by mass percentage: 2%-3% of tris(hydroxymethyl)aminomethane, 1%-2% of boric acid, 2%-3% of hydrogen peroxide, 0.5%-0.7% of sodium dodecyl sulfate, 8%-12% of sodium chloride, 0.03%-0.08% of dithiothreitol, 10%-15% of methanol, 2%-3% of a stabilizer and 0.05%-0.1% of a preservative.

[0069] The preferred specific embodiments of the present invention are described in detail above. It should be understood that a person skilled in the art can make many modifications and changes based on the concept of the present invention without creative work. Therefore, any technical solution that can be obtained by a person skilled in the art through logical analysis, reasoning or limited experiments based on the concept of the present invention on the basis of the prior art should be within the scope of protection determined by the claims.

Claims

1. A hair extract for drug detection, characterized in that: The invention comprises the following components by mass percentage: 1%-5% of tris(hydroxymethyl)aminomethane, 0.5%-3% of boric acid, 1%-4% of hydrogen peroxide, 0.1%-1% of sodium dodecyl sulfate and 5%-15% of sodium chloride.

2. The hair extract for drug detection according to claim 1, characterized in that: The hair extract for drug detection comprises the following components by mass percentage: 2%-3% of tris(hydroxymethyl)aminomethane, 1%-2% of boric acid, 2%-3% of hydrogen peroxide, 0.5%-0.7% of sodium dodecyl sulfate, and 8%-12% of sodium chloride.

3. The hair extract for drug detection according to claim 1, characterized in that: The hair extract for drug detection also includes dithiothreitol; the mass percentage of the dithiothreitol is 0.01%-0.1%.

4. The hair extract for drug detection according to claim 1, characterized in that: The hair extract for drug detection also includes methanol; the mass percentage of the methanol is 5-20%.

5. The hair extract for drug detection according to claim 1, characterized in that: The hair extract for drug detection also includes a stabilizer and a preservative; the mass percentage of the stabilizer is 1%-5%; the mass percentage of the preservative is 0.01%-0.2%.

6. The hair extract for drug detection according to claim 5, characterized in that: The stabilizer is ethylenediaminetetraacetic acid or citric acid.

7. The hair extract for drug detection according to claim 5, characterized in that: The preservative is sodium azide or sodium formate.

8. A method for preparing a hair extract for drug detection, characterized in that: The following steps are involved: S1. Preparation: including material preparation and equipment preparation; The material preparation is to collect and prepare all the required raw materials, including tris(hydroxymethyl)aminomethane, boric acid, hydrogen peroxide, sodium lauryl sulfate, sodium chloride, dithiothreitol, methanol, and preservatives and stabilizers planned to be added; The equipment preparation includes preparing a weighing device, a stirring device, a pH measuring device, a solid raw material container, a liquid raw material container, a pipetting device and a liquid measuring device; S2, preparing a basic solution, comprising the following steps: Weighing: Use a weighing device to accurately weigh a certain amount of solid raw materials such as tris(hydroxymethyl)aminomethane, boric acid and sodium chloride, and place them in a solid raw material container; Dissolution: Add an appropriate amount of distilled water into the solid raw material container and stir thoroughly using a stirring device until all the solid raw materials are completely dissolved. Adjusting pH value: Use a pH measuring device to detect the pH value of the base solution, and add an appropriate amount of acid or alkaline solution to adjust it as needed to ensure that the pH value is within the predetermined range; S3, adding active ingredients, comprising the following steps: Add hydrogen peroxide: slowly add a predetermined amount of hydrogen peroxide to the base solution while stirring, and control the adding speed to avoid violent reaction; Add sodium lauryl sulfate: Continue stirring and slowly add the predetermined amount of sodium lauryl sulfate; the addition of sodium lauryl sulfate will increase the viscosity and foam volume of the solution, so keep stirring to ensure it is evenly dispersed; Add dithiothreitol: first mix dithiothreitol with distilled water to dissolve it, then add the solvent to the main solution; keep stirring during the addition process; Add preservatives and stabilizers; mix and dissolve the preservatives and stabilizers with distilled water, and then add the dissolved solvent to the solution; keep stirring during the addition process; S4, adding methanol. Slowly pour a predetermined amount of methanol into the solution while stirring. Since methanol is miscible with water, local heat release may occur when it is added. Therefore, the addition speed needs to be controlled and stirring needs to be continued to dissipate heat. S5, volume setting and mixing; Make up to volume: Use distilled water to adjust the solution volume to the desired value and continue stirring to ensure that all ingredients are evenly mixed; Mix well: Place the solution on a stirring device and continue stirring for a while to ensure that all ingredients are fully dissolved and mixed well; S6. Inspection and storage: Inspection: Conduct necessary inspections on the prepared extract, such as pH re-inspection, appearance inspection, etc., to ensure that it meets the quality requirements; Storage: Transfer the extract to a clean glass or plastic bottle and seal it in a cool, dry, dark place away from fire and heat sources.