A medical experimental staining device for studying the elongation of human styloid process

Abstract

The invention discloses a medical experimental staining device for studying excessively long human styloid processes, comprising a chassis, a moving mechanism, a plurality of dye boxes and a flushing water pipe arranged in the chassis, the moving mechanism comprising a longitudinal moving mechanism arranged along the width direction of the chassis, a transverse moving mechanism mounted on the longitudinal moving mechanism and a vertical moving mechanism arranged below the transverse moving mechanism, the vertical moving mechanism vertically provided with an adapter seat toward the front side of the chassis, a glass slide placement rack installed on the adapter seat, a cross bar provided on the glass slide placement rack, a plurality of placement seats rotatably connected on the cross bar, the placement seats move in the vertical direction through their movably connected angle adjustment components to drive the placement seats to rotate on the cross bar to adjust the angle, the dye box is arranged at the bottom of the chassis, and the flushing water pipe is arranged on the wide side of one side of the chassis. The invention solves the technical problem that the existing clamp cannot control the synchronous tilting and rotation of multiple glass slides, resulting in different flushing effects of multiple slices in the same time, which is not conducive to subsequent synchronous staining.

Description

Technical Field

[0001] The invention belongs to the technical field of slide staining equipment and relates to a medical experimental staining device for studying excessive elongation of human styloid processes. Background Art

[0002] Eagle's syndrome refers to a series of symptoms caused by abnormal styloid process morphology, length, orientation and / or stylohyoid ligament ossification, called elongated styloid processes or styloid process syndrome (SPS). The clinical manifestations of elongated styloid process syndrome depend on the length, angle and width of the styloid process. Symptoms can range from mild discomfort to severe vascular and neurological complications. Compression of the lower cranial nerves in the neck can cause persistent throat pain, ear pain, dysphagia, cervical rotational pain, facial pain, vertigo, syncope, and headache, becoming a common outpatient disease. Carotid artery dissection (CAD) refers to a sudden tear in the intima of the artery caused by various reasons, circulating blood enters the middle layer of the blood vessel wall and causes its stratification, and gradually expands in the middle layer of the artery to form a dissection as driven by blood flow pressure. Acute onset and acute ischemia of organs are the characteristics of this disease.

[0003] The internal carotid artery is adjacent to the styloid process and ascends behind the styloid process. An excessively long styloid process can compress the blood vessels, causing vascular damage or carotid artery dissection, leading to arterial stenosis and occlusion, and then transient ischemic attack or cerebral infarction. The mechanism includes mechanical compression of the styloid process causing arterial stenosis; mechanical stimulation / impact damage to the arterial wall leading to thromboembolism or carotid artery dissection. The incidence of people with an excessively long styloid process (4% to 7%), among which the proportion of styloid process syndrome reaches 10% (including ischemic and non-ischemic symptoms). Therefore, strengthening the understanding of this disease, paying attention to the diagnosis and treatment of styloid process syndrome, timely surgical removal of the excessively long styloid process, and relieving mechanical contact are the key to preventing severe stroke recurrence.

[0004] Animal models play a vital role in clarifying the pathological mechanism of the disease. Carotid artery dissection caused by excessive styloid process is an important cause of stroke in young people. The study of its pathological mechanism and treatment methods requires a suitable animal model. When constructing an animal model, the internal carotid artery of the modeling group mice with internal carotid artery dissection aneurysm and the same part of the internal carotid artery of the control group rats were used for embedding and frozen sectioning, and then staining and elastic fiber staining experiments were performed to determine whether the internal carotid artery dissection model of the modeling group rats was successfully established.

[0005] In the process of staining slices with a staining device in the prior art, a clamp is used to immerse the slide containing the slices in a stain to stain the slices, and clean water is used to rinse off the excess stain on the slices after staining. In order to improve the rinsing efficiency, the rinse area of ​​the slice surface with clean water is increased, so that the clean water can more easily flow through the entire slice surface and increase the retention time of the water flow on the slice surface. During the rinsing process, the slide is driven to tilt by the clamp to change the angle between the water flow and the slice to complete the rinsing.

[0006] In order to improve the staining efficiency of slices, slices on multiple slides are usually stained and rinsed synchronously. However, the clamp in the prior art cannot control the synchronous tilting and rotation of multiple slides, resulting in different rinse effects of multiple slices at the same time, which is not conducive to subsequent synchronous staining. Summary of the invention

[0007] In view of the shortcomings of the prior art, the present invention provides a medical experimental staining device for studying the excessive length of human styloid process, which solves the technical problem that the clamp in the prior art cannot control the synchronous tilting and rotation of multiple slides, resulting in different washing effects of multiple slices in the same time, which is not conducive to subsequent synchronous staining.

[0008] The technical solution provided by the present invention is as follows:

[0009] The invention provides a medical experimental staining device for studying excessive length of human styloid process, comprising a chassis, wherein a moving mechanism, a plurality of dye boxes and a flushing water pipe are arranged in the chassis, wherein the moving mechanism comprises a longitudinal moving mechanism arranged along the width direction of the chassis, a transverse moving mechanism mounted on the longitudinal moving mechanism and a vertical moving mechanism arranged below the transverse moving mechanism, wherein the vertical moving mechanism is vertically provided with an adapter seat toward the front side of the chassis, a slide placement rack is installed on the adapter seat, a cross bar is provided on the slide placement rack, a plurality of placement seats are rotatably connected to the cross bar, the placement seats are moved in the vertical direction through their movably connected angle adjustment components to drive the placement seats to rotate on the cross bar to achieve angle adjustment, a plurality of dye boxes are arranged at the bottom of the chassis, and the flushing water pipe is arranged at a wide side of one side of the chassis and is located above one of the dye boxes.

[0010] Furthermore, the placement seat includes a first mounting seat and a second mounting seat, the first mounting seat and the second mounting seat are connected by a fixed shaft, the first mounting seat is rotatably connected to the cross bar, the angle adjustment assembly includes an adjustment seat connected to the adapter seat through a vertical drive, the adjustment seat is arranged below the cross bar, the second mounting seat is provided with an inclined guide groove, the adjustment seat is fixedly provided with a connecting shaft, and the connecting shaft is movably arranged in the guide groove.

[0011] Furthermore, a through placement hole is provided on the first mounting seat, and a groove is provided on the second mounting seat corresponding to the placement hole. The aperture of the placement hole is larger than the thickness of the glass slide. After the glass slide passes through the placement hole, the bottom of the glass slide contacts the bottom wall of the groove of the second mounting seat.

[0012] Furthermore, a water guide hole is provided on the inner wall of the groove of the second mounting seat.

[0013] Furthermore, the longitudinal moving mechanism is provided with two parts including a first fixed seat, a first threaded rod and a first movable seat, the first fixed seat is arranged along both sides of the wide side of the chassis, the first threaded rod is rotatably arranged on the first fixed seat, the first movable seat is slidably arranged on the first fixed seat and is threadedly connected to the first threaded rod, the transverse moving mechanism includes a second fixed seat, a second threaded rod and a second movable seat, the second fixed seat is arranged on the first movable seat, the second movable seat is slidably arranged on the second fixed seat and is threadedly connected to the second threaded rod, and the vertical moving mechanism is fixedly arranged on the second movable seat.

[0014] Furthermore, support shafts are provided on both sides of the adapter seat, and rectangular seats are fixedly provided at the ends of the two support shafts away from each other. Rectangular holes are opened on the slide placement rack, and the rectangular seats are clamped in the corresponding rectangular holes.

[0015] Furthermore, it also includes a sealing mechanism, which includes a cover glass rack arranged on the front inner wall of the chassis and a control box on the rear inner wall, the control box is connected to multiple rotating structures above and a linear drive structure is fixed below, the multiple rotating structures are connected to clamps, and the linear drive structure is connected to a third movable seat for adjusting the opening sizes of multiple clamps, and the cover glass rack is provided with a plurality of inclined support frames, and when sealing is performed, the slide rack moves between the cover glass rack and the control box.

[0016] Furthermore, the multiple rotating structures include multiple rotating drivers and multiple driving shafts which are respectively connected to the top wall of the control box through a first connecting column and a second connecting column. The output shaft of the rotating driver is fixedly arranged on the driving shaft, the driving shaft is rotatably connected to the clamp, and the multiple driving shaft sleeves are provided with sprockets, and the corresponding two sprockets are connected through the same chain transmission.

[0017] Furthermore, a plurality of rotating seats are installed on the third movable seat, and positioning holes are provided on the rotating seats, and the clamps are inserted into the positioning holes.

[0018] Furthermore, the clamp is two elastic clamping pieces fixed together at one end.

[0019] Beneficial Effects

[0020] The present invention provides an angle adjustment component. After the slice samples on the slide are stained, when the samples are rinsed with clean water, the vertical movement of the angle adjustment component can drive multiple slides to rotate synchronously. During rinsing, the angle between the water flow and the slices is adjusted to ensure that the rinsing effects of different slices are the same, which is beneficial to subsequent synchronous staining.

[0021] The present invention sets a sealing mechanism to make the slide glass in an inclined state, and uses a clamp to pick up the slide glass to complete the packaging. When sealing, not only can the bubbles between the slide glass and the cover glass be removed, but multiple cover glass slides can also be sealed simultaneously, reducing the workload of the staff. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a schematic diagram of the three-dimensional structure of the dyeing device of the present invention.

[0023] Figure 2 It is a schematic diagram of the cross-sectional structure of the chassis of the present invention from the front view.

[0024] Figure 3 It is a schematic diagram of the three-dimensional structure of the longitudinal moving mechanism of the present invention.

[0025] Figure 4 It is a schematic diagram of the three-dimensional structure of the lateral moving mechanism of the present invention.

[0026] Figure 5 It is a schematic diagram of the front view structure of the vertical moving mechanism of the present invention.

[0027] Figure 6 It is a schematic diagram of the main structure of the placement seat of the present invention.

[0028] Figure 7 It is a schematic cross-sectional structural diagram of the placement seat of the present invention.

[0029] Figure 8 It is a structural schematic diagram of the rotation trajectory of the placement seat of the present invention.

[0030] Fig. 9 It is a schematic cross-sectional structural diagram of the control box of the present invention.

[0031] Fig.10 For the present invention Fig. 9 Schematic diagram of the side view of the cover glass placement rack.

[0032] Fig.11 For the present invention Fig. 9 Schematic diagram of the side view structure of the middle moving seat.

[0033] Fig.12 For the present invention Fig. 9 Schematic diagram of the structure of the middle sprocket from the side.

[0034] Fig.13It is a structural schematic diagram of the encapsulation track of the cover glass encapsulated onto the slide glass of the present invention.

[0035] Fig.14 It is a schematic diagram of the process of the dyeing method of the present invention.

[0036] Explanation of the reference numerals in the accompanying drawings: 1. chassis; 11. flushing water pipe; 12. cover glass rack; 2. dye box; 3. longitudinal moving mechanism; 31. first fixed seat; 32. first threaded rod; 33. first movable seat; 41. lateral moving mechanism; 42. vertical moving mechanism; 421. adapter seat; 5. angle adjustment assembly; 51. slide rack; 52. placement seat; 521. guide groove; 53. vertical drive; 54. adjustment seat; 541. connecting shaft; 6. sealing mechanism; 61. control box; 62. drive shaft; 63. clamp; 64. third movable seat; 641. rotating seat; 642. positioning hole; 65. sprocket; 651. chain; 66. rotating drive; 67. linear drive structure; a. slide; b. cover glass. DETAILED DESCRIPTION

[0037] The present invention will be further described below in conjunction with the accompanying drawings. The following embodiments are only used to more clearly illustrate the technical solution of the present invention, and cannot be used to limit the protection scope of the present invention.

[0038] In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and the like are only used for descriptive purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, features defined as "first", "second", and the like may explicitly or implicitly include one or more of the features. In the description of the present invention, unless otherwise specified, "multiple" means two or more.

[0039] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood by specific circumstances.

[0040] Embodiment 1, as Figures 1 to 8 As shown, an embodiment of the present invention provides a medical experimental staining device for studying excessive length of human styloid process, including a chassis 1, wherein a moving mechanism, a plurality of dye boxes 2 and a flushing water pipe 11 are arranged in the chassis 1, wherein the moving mechanism includes a longitudinal moving mechanism 3 arranged along the width direction of the chassis 1, a transverse moving mechanism 41 mounted on the longitudinal moving mechanism, and a vertical moving mechanism 42 arranged below the transverse moving mechanism 41, wherein the vertical moving mechanism 42 is vertically provided with an adapter seat 421 toward the front side of the chassis 1, and a slide placement rack 51 is installed on the adapter seat 421, and a cross bar is provided on the slide placement rack 51, and a plurality of placement seats 52 are rotatably connected to the cross bar, wherein the placement seat 52 moves in the vertical direction through its movably connected angle adjustment component 5 to drive the placement seat 52 to rotate on the cross bar to adjust the angle, and the plurality of dye boxes 2 are arranged at the bottom of the chassis 1, and the flushing water pipe 11 is arranged at the wide side of one side of the chassis 1 and above one of the dye boxes 2.

[0041] The present invention provides an angle adjustment component. After the slice samples on the slide are stained, when the samples are rinsed with clean water, the vertical movement of the angle adjustment component can drive multiple slides to rotate synchronously. During rinsing, the angle between the water flow and the slices is adjusted to ensure that the rinsing effects of different slices are the same, which is beneficial to subsequent synchronous staining.

[0042] Example 2: In the study of human styloid process elongation, it is crucial to construct a rat model of arterial dissection associated with styloid process elongation, which includes the following steps:

[0043] Step 1: 14 healthy adult male Wistar rats with a body weight range of 200 g to 250 g were selected as subjects for model construction and randomly divided into two groups.

[0044] Step 2: General anesthesia was used to make the rats pain-free. Then, the skin of the neck was cut open and the styloid process and carotid artery were exposed under aseptic operation.

[0045] Step 3: Use precise microsurgical tools to lengthen the styloid process of the experimental group of rats. Without damaging the surrounding tissues, gradually cut off the connection between the styloid process and the surrounding structures, and use biocompatible material bone cement to lengthen the styloid process so that it increases in length and abuts against the wall of the internal carotid artery.

[0046] Step 4: Close the incision and provide recovery care for the rats. During this period, closely monitor the rats' vital signs to ensure their health. The rats in the control group were raised normally.

[0047] Step 5: Fix the trunk and head of the rats with a fixator, and give the two groups of rats different times and frequencies of head lowering, head raising and neck turning movements for four weeks to simulate different pathological conditions. At the same time, record the behavioral performance and physiological data of the rats. Record the cerebral embolism, death time and mortality rate of the rats in the modeling group, and perform autopsies on the dead rats to observe whether the rats in the modeling group died of cerebral embolism related to carotid artery dissection.

[0048] At the end of the fourth week of feeding, the remaining surviving rats in the model group and the control group were killed by overdose of sodium pentobarbital anesthesia, and the internal carotid arteries of the killed rats in the model group and the control group were separated using a microscopic instrument using a 4% mass percentage paraformaldehyde solution. The occurrence of internal carotid artery dissection in the model group and the control group was observed and counted.

[0049] The same parts of the internal carotid arteries of the model group mice with internal carotid artery dissection and the internal carotid arteries of the control group rats were respectively embedded and frozen sectioned, and then dyeing and elastic fiber staining experiments were performed to determine whether the internal carotid artery dissection model of the model group rats was successfully established.

[0050] The staining experiment specifically includes the following process: paraffin section slide a fixed with paraformaldehyde is dewaxed and stained with water and hematoxylin solution for 5 minutes, then rinsed with tap water for 2 minutes, then separated with 0.5% hydrochloric acid alcohol for 2 to 3 seconds, then rinsed with tap water for blueing for 15 to 30 minutes, then stained with eosin solution (95%) ethanol solution for 1 minute, then rinsed with distilled water for 1 to 2 seconds, then rinsed with 80% ethanol for 1 to 2 seconds, then washed with 95% ethanol for 2 to 3 seconds, then washed with 100% ethanol for 3 to 5 seconds, then soaked in xylene for 5 minutes, and soaked 3 times. Finally, neutral gum is used to seal the coverslip b and slide a.

[0051] When conducting dyeing experiments, in order to improve dyeing efficiency and reduce the workload of staff, such as Figures 1 to 8 An embodiment of the present invention provides a medical experimental staining device for studying excessive styloid process in humans, including a chassis 1, a flushing water pipe 11 is arranged in the chassis 1, a water pump is fixedly connected to the flushing water pipe 11, a plurality of dye boxes 2 are arranged in the chassis 1, and a dye is contained in the dye box 2.

[0052] A longitudinal moving mechanism 3 is arranged in the chassis 1, and the longitudinal moving mechanism 3 includes a first moving seat 33, a transverse moving mechanism 41 is arranged on the first moving seat 33, a vertical moving mechanism 42 is arranged on the transverse moving mechanism 41, an adapter seat 421 is arranged at the output end of the vertical moving mechanism 42, a slide placement rack 51 is installed on the adapter seat 421, a cross bar is arranged on the slide placement rack 51, a plurality of placement seats 52 are rotatably arranged on the cross bar, and a slide a is placed in the placement seat 52; the adapter seat 421 drives the slide placement rack 51 to move vertically through the vertical moving mechanism 42 to place the slide a containing the slice into the dye box 2 for dyeing, and the adapter seat 421 drives the slide placement rack 51 to move horizontally through the longitudinal moving mechanism 3 and the transverse moving mechanism 41 to replace the dye box 2 or be located under the flushing water pipe 11, and the slide placement rack 51 is provided with an angle adjustment component 5, and the placement seat 52 is driven to rotate around the cross bar by the vertical movement of the angle adjustment component 5 to adjust the angle.

[0053] like Figures 1 to 4 In order to improve the driving efficiency of the adapter seat 421, specifically, the longitudinal moving mechanism 3 includes a first fixed seat 31, the first fixed seat 31 is fixedly arranged on both sides of the width direction of the chassis 1, a first threaded rod 32 is rotatably arranged on the first fixed seat 31, a first movable seat 33 is slidably arranged on the first fixed seat 31, and the first threaded rod 32 is threadedly connected with the first movable seat 33, the lateral moving mechanism 41 includes a second fixed seat, a second threaded rod is rotatably arranged on the second fixed seat, the second movable seat is slidably arranged on the second fixed seat, the second threaded rod is threadedly connected with the second movable seat, and the vertical moving mechanism 42 is fixedly arranged on the second movable seat.

[0054] It should be noted that a first motor is fixedly arranged on the first fixed seat 31, and the output shaft of the first motor is fixedly arranged on the first threaded rod 32; a second motor is fixedly arranged on the second fixed seat, and the output shaft of the second motor is fixedly arranged on the second threaded rod; the vertical moving mechanism 42 is arranged as a linear motor; the adapter seat 421 is fixedly arranged on the output shaft of the linear motor and is arranged perpendicularly to the vertical moving mechanism 42 toward the front side of the chassis.

[0055] It should also be noted that the first movable seat 33 is driven to move by the rotation of the output shaft of the first motor, and the second movable seat is driven to move by the rotation of the output shaft of the second motor.

[0056] It should be noted that one end of the water pump away from the flushing water pipe 11 is fixedly connected to a water tank, and clean water is contained in the water tank. A plurality of drainage holes are arranged in the drainage end of the flushing water pipe 11, and two adjacent drainage holes are arranged at equal intervals. Due to the arrangement of the drainage holes, when the clean water is discharged from the drainage end of the flushing water pipe 11, the water flow becomes a plurality of water columns, and each group of water columns corresponds to a corresponding placement seat 52. When rinsing the slide a, the use of water resources can be reduced, thereby achieving the effect of saving water.

[0057] It should also be noted that the multiple dye boxes 2 contain ethanol of different concentration gradients, different dyes and required reagents, and each dye box 2 is fixedly connected to a guide tube, on which a gate valve is installed. After the dyeing experiment, the reagents in the dye box 2 need to be replaced regularly. The dye box 2 is placed directly below the flushing water pipe 11, and the dye box 2 here is used to receive the waste liquid after washing the slices. The installation between the adapter 421 and the slide rack 51 is not in the same vertical plane, that is, when the adapter 421 drives the slide rack 51 to be located below the flushing water pipe 11, there will be no interference, and the water discharged from the drainage end of the flushing water pipe 11 can rinse the slide a.

[0058] In this embodiment, the placement seat 52 includes a first mounting seat and a second mounting seat, and the first mounting seat and the second mounting seat are connected by the same fixed axis. The center of the first mounting seat is rotatably arranged between the slide placement rack 51. The first mounting seat is provided with a placement hole, and the second mounting seat is provided with a groove corresponding to the placement hole. The aperture of the placement hole is larger than the thickness of the slide a. After the slide a passes through the placement hole, the bottom of the slide a contacts the bottom wall of the groove of the second mounting seat. The inner wall of the second mounting seat is provided with a water guide hole. When washing the slice sample on the slide a, the water in the second mounting seat is discharged through the water guide hole.

[0059] like Figures 5 to 8 In order to further improve the synchronous control of multiple glass slides a and reduce the use of driving sources to reduce the production cost of the equipment, specifically, the angle adjustment component 5 includes an adjustment seat 54 connected to the adapter seat 421 through a vertical driver 53, and the adjustment seat 54 is arranged below the cross bar. An inclined guide groove 521 is provided on the second mounting seat, and a connecting shaft 541 is fixedly provided on the adjustment seat, and the connecting shaft 541 is movably arranged in the guide groove.

[0060] It should be noted that the guide groove 521 is tilted on the second mounting seat, and the connecting shaft 541 is movably arranged in the guide groove 521. During the vertical upward movement of the adjustment seat 54, the connecting shaft 541 is driven to move upward. During the movement of the connecting shaft 541, the placement seat 52 will produce an angular displacement through the guide groove 521, that is, the effect of synchronous tilting and rotating of multiple placement seats 52 is achieved. The placement seat 52 can be controlled to swing back and forth by controlling the reciprocating up and down movement of the adjustment seat 54.

[0061] like Figures 2 to 8 In order to facilitate the adjustment of the posture of the slide rack 51 and the disassembly of the slide rack 51, specifically, a vertical driver 53 is rotatably arranged on the adapter seat 421, a connecting seat is fixedly arranged on the adjustment seat 54, the output shaft of the vertical driver 53 is fixedly arranged with the connecting seat, support shafts are arranged on both sides of the adapter seat 421, and rectangular seats are fixedly arranged at one end of the two support shafts away from each other, and a rectangular hole is opened on the slide rack 51, and the rectangular seat is clamped in the corresponding rectangular hole.

[0062] It should be noted that the vertical drive 53 is configured as a cylinder, and the output shaft of the cylinder is fixed on the adjustment seat 54 by screws. The raw material of the slide rack 51 is set to plastic, and the plastic is elastic. The slide rack 51 can be removed from the support shaft through the connection between the rectangular hole and the rectangular seat. Two motors are fixed on the adapter seat 421, and the support shaft is fixed on the output shaft of the motor. After starting the motor, the slide rack 51 can be driven to rotate, and the efficiency of staining the sliced ​​sample on the slide a can be further improved by coordinating the tilting rotation of the placement seat 52.

[0063] The specific implementation scenario is as follows: when the glass slide a needs to be rinsed after being stained in the dye box 2, the adapter seat 421 moves to place the glass slide placement rack 51 under the rinsing water pipe 11, and then starts the water pump, and the clean water is discharged through the drainage end of the rinsing water pipe 11, and then drives the adjustment seat 54 to move upward. During the upward movement of the adjustment seat 54, the multiple placement seats 52 are pushed to rotate synchronously, and the rotation of the placement seat 52 causes the glass slide a to rotate. Compared with the prior art, the effect of synchronously changing the angle between the slices on multiple glass slides a and the water flow can be achieved, and multiple The angle of the slide a is adjusted synchronously. Compared with the vertical placement of the slide a for washing, the tilted slide a allows the water flow to flow along the entire surface. Compared with the situation where the water flow is mainly concentrated on the top when placed vertically, the entire surface of the slide a can be washed more evenly, and the water flow stays on the inclined surface of the slide a for a longer time, which increases the contact time between the water and the slice sample, which is conducive to more thorough cleaning. Compared with the water flow directly hitting the slide when placed vertically, the tilted placement can reduce water splashing, reduce the risk of contamination, and reduce mechanical damage to fragile slice samples. In addition, during the dyeing process, when the slide a is located in the dye box 2, multiple slides a can also be driven to perform synchronous angle tilting and swinging in the dye box 2, which can increase the fluidity between the dye and the slice sample on the slide a, effectively increase the contact opportunity between the slice sample and the dye, significantly improve the flow and penetration of the dye, reduce bubble interference, accelerate the dyeing reaction speed, avoid the occurrence of uneven dyeing, and further improve the dyeing effect.

[0064] After the slice sample on the slide a is stained, the sample needs to be sealed with a cover glass b. There are two common sealing methods. One is to add neutral resin to the slice sample on the slide a, and then cover the cover glass b on the slice sample to complete the sealing; the second method is to add neutral resin on one side of the cover glass b and then cover the cover glass b on the slice sample to complete the sealing. In method one, more overflow of neutral resin will be generated after the cover glass b is covered on the slide a for sealing. The excess neutral resin needs to be wiped off after sealing. The sealing method in method two can reduce the use of sealing resin, thereby avoiding the step of wiping off excess neutral resin after sealing. Both the sealing methods of method one and method two require manual operation. In order to reduce the workload of the staff, the sealing operation is automatically completed after the sample on the slide a is stained.

[0065] In this embodiment, it also includes a sealing mechanism, which includes a cover glass rack 12 arranged on the front inner wall of the chassis 1 and a control box 61 on the rear inner wall. The control box 61 is connected to multiple rotating structures on the top and a linear drive structure 67 is fixed on the bottom. The multiple rotating structures are connected to clamps 63, and the linear drive structure 67 is connected to a third movable seat 64 for adjusting the opening sizes of the multiple clamps. The cover glass rack 12 is provided with a plurality of inclined support frames. When sealing is performed, the slide rack 51 moves between the cover glass rack 12 and the control box 61.

[0066] In this embodiment, the multiple rotating structures include multiple rotating drivers 66 and multiple driving shafts 62, which are respectively connected to the inner top wall of the control box 61 through the first connecting column and the second connecting column. The output shaft of the rotating driver 66 is fixedly arranged with the driving shaft 62, and the driving shaft 62 is rotatably connected to the clamp 63. The multiple driving shafts 62 are provided with sprockets 65, and the corresponding two sprockets 65 are connected by the same chain 651.

[0067] Specifically, refer to Figures 2 to 13 A sealing mechanism 6 is provided in the chassis 1, and the sealing mechanism 6 includes a cover glass rack 12 fixedly arranged on the front inner wall of the chassis 1 and a control box 61 arranged on the rear inner wall of the chassis 1. When the sealing operation is performed, the slide rack 51 is located between the cover glass rack 12 and the control box 61, and the cover glass b is placed on the cover glass rack 12.

[0068] It should also be noted that a plurality of inclined support frames are provided on the cover glass placement rack 12, and the inclined support frames are used to support the cover glass b.

[0069] In this embodiment, neutral resin is dripped on one side of cover glass b so that the neutral resin is evenly distributed on cover glass b. Since neutral resin has surface tension and the weight of neutral resin on cover glass b is small, even if cover glass b is placed vertically, the neutral resin on its surface can adhere and will not fall off. Cover glass b dripped with neutral resin is tilted and placed on cover glass placement rack 12, and the side of cover glass b that is not dripped with neutral resin is supported by the support frame on cover glass placement rack 12. After the slice sample on slide glass a is stained, it is left to stand to dry, and then slide glass a is driven to be in a tilted state, and the clamp 63 is driven to clamp the edge of cover glass b, and then cover glass b is brought close to the slice sample on slide glass a, so that the slice sample on slide glass a and the side of cover glass b that is dripped with neutral resin are close to each other, and the bottom of cover glass b is driven to contact with slide glass a first, as shown in FIG. Fig.13 As shown, the cover glass b is then driven to rotate slowly so that the cover glass b is gradually attached to the slide glass a from bottom to top to complete the sealing of the slide glass a and the cover glass b. Compared with the method of placing the slide glass a horizontally and then completing the sealing of the cover glass b in the prior art, in this scheme, the slide glass a is first tilted, and then the cover glass b is attached to the slide glass a from bottom to top to complete the sealing. The bottom of the cover glass b first contacts the slide glass a, and as the cover glass b approaches the slide glass a as a whole from bottom to top, during this process, the neutral resin on the cover glass b remains between the slide glass a and the cover glass b, and the angle between the cover glass b and the slide glass a becomes smaller and smaller, so that the bubbles in the neutral resin of the cover glass b can be squeezed and discharged, thereby avoiding residual bubbles between the slide glass a and the cover glass b during packaging. Not only can the sealing operation be automatically completed to reduce the workload of the staff, but also residual bubbles between the slide glass a and the cover glass b can be avoided during the sealing process.

[0070] like Figures 9 to 12 As shown, after the slice samples on multiple slides a are stained, in order to be able to perform synchronous sealing operations on multiple slices and improve the sealing efficiency in the experiment, specifically, multiple driving shafts 62 are rotatably arranged in the control box 61, a third movable seat 64 is slidably arranged in the control box 61, and multiple rotating seats 641 are rotatably arranged in the third movable seat 64. Positioning holes 642 are opened on the rotating seat 641, and the clamp 63 is located in the corresponding positioning holes 642.

[0071] In this embodiment, the clamp 63 includes two clamping plates, one end of which is fixed together, and the clamping plates are set to elastic material and have elasticity. The elastic material includes but is not limited to metal. The combination of the two clamping plates in the clamp 63 is used similarly to "tweezers". When pressure is applied to the two clamping plates, the two clamping plates move closer to each other, and when the pressure is released, the two clamping plates move away from each other.

[0072] In this embodiment, the clamp 63 is located in the positioning hole 642, and the rotation of the clamp 63 can drive the rotating seat 641 to rotate synchronously. When clamping the cover glass b, the third movable seat 64 is moved toward one side of the cover glass b. Since the two clamping pieces of the clamp 63 are initially in an open state, when the clamp 63 is driven to move, the two clamping pieces of the clamp 63 are brought close to each other to clamp the cover glass b under the action of the positioning hole 642. After the clamp 63 clamps the cover glass b, the clamp 63 is driven to rotate to make the cover glass b fit with the slide a, thereby completing the packaging of the cover glass b and the slide a.

[0073] like Figures 9 to 12 In order to drive multiple clamps 63 to rotate synchronously, specifically, multiple rotation drivers 66 are fixedly arranged in the control box 61, and the output shaft of the rotation driver 66 is fixedly arranged with the driving shaft 62. A linear driving structure 67 is also fixedly arranged in the control box 61, and the output shaft of the linear driving structure 67 is fixedly arranged with the third movable seat 64. Sprockets 65 are fixedly sleeved on the multiple driving shafts 62, and the corresponding two sprockets 65 are connected through the same chain 651.

[0074] It should be noted that the rotary driver 66 is configured as a motor, the output shaft of the motor is fixedly disposed on the driving shaft 62 , and the linear driving structure 67 is configured as a cylinder, the output shaft of the cylinder is fixedly disposed on the third movable seat 64 .

[0075] It should also be noted that starting the linear drive structure 67 can drive the third movable seat 64 to move. After starting the rotary driver 66, the output shaft of the rotary driver 66 drives the sprocket 65 to rotate, and the sprocket 65 drives the chain 651. In conjunction with the movement of the third movable seat 64, it can not only achieve the synchronous clamping of multiple clamps 63, but also achieve the synchronous rotation of multiple clamps 63.

[0076] It should be noted that a fourth linear drive is fixedly installed in the chassis 1 . The fourth linear drive is configured as a linear motor. The control box 61 is fixedly disposed on the output end of the linear motor. The fourth linear drive can drive the control box 61 to move in the chassis 1 .

[0077] like Fig.14 As shown, a staining method for a medical experimental staining device for studying excessive elongation of human styloid process comprises the following steps:

[0078] Step 1: Place the glass slide a containing the slices in the placement seat 52, and the first movable seat 33 and the second movable seat drive the adapter seat 421 to move horizontally and vertically to place the glass slide a in the dye box 2 to dye the slices;

[0079] Step 2: Then move the glass slide a to the bottom of the flushing water pipe 11, start the vertical driver 53, the output shaft of the vertical driver 53 moves back and forth vertically to drive the glass slide a to rotate back and forth, and start the water pump to make clean water flow out of the flushing water pipe 11 to flush the residual stain on the glass slide a;

[0080] Step 3: Repeat steps 1 and 2, and put the slide a into different dye boxes 2 and use different dyes to dye;

[0081] Step 4: After the staining of the glass slide a is completed, the glass slide a is kept in an inclined posture, and the cover glass b with the encapsulation glue added is clamped by the clamp 63, and the cover glass b is encapsulated on the slice of the glass slide a.

[0082] The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A medical experimental staining device for studying excessive elongation of human styloid process, characterized in that: The invention comprises a chassis, in which a moving mechanism, a plurality of dye boxes and a flushing water pipe are arranged, the moving mechanism comprising a longitudinal moving mechanism arranged along the width direction of the chassis, a transverse moving mechanism mounted on the longitudinal moving mechanism and a vertical moving mechanism arranged below the transverse moving mechanism, the vertical moving mechanism vertically provided with an adapter seat toward the front side of the chassis, a glass slide placement rack is installed on the adapter seat, a cross bar is provided on the glass slide placement rack, a plurality of placement seats are rotatably connected to the cross bar, the placement seat moves in the vertical direction through its movably connected angle adjustment component to drive the placement seat to rotate on the cross bar to adjust the angle, a plurality of dye boxes are arranged at the bottom of the chassis, the flushing water pipe is arranged at the wide side of one side of the chassis and is located above one of the dye boxes; the placement seat comprises a first mounting seat and a second mounting seat, the first mounting seat The seat and the second mounting seat are connected by a fixed shaft, the first mounting seat is rotatably connected to the cross bar, the angle adjustment assembly includes an adjustment seat connected to the adapter seat through a vertical drive, the adjustment seat is arranged below the cross bar, the second mounting seat is provided with an inclined guide groove, the adjustment seat is fixedly provided with a connecting shaft, and the connecting shaft is movably arranged in the guide groove; a through placement hole is provided on the first mounting seat, and a groove is provided on the second mounting seat corresponding to the placement hole, the aperture of the placement hole is larger than the thickness of the glass slide, and the bottom of the glass slide contacts the bottom wall of the groove of the second mounting seat after passing through the placement hole; support shafts are provided on both sides of the adapter seat, and a rectangular seat is fixedly provided at one end of the two support shafts away from each other, a rectangular hole is provided on the glass slide placement rack, and the rectangular seat is clamped in the corresponding rectangular hole.

2. The medical experimental staining device for studying excessive elongation of human styloid process according to claim 1, characterized in that: A water guide hole is provided on the inner wall of the groove of the second mounting seat.

3. The medical experimental staining device for studying excessive elongation of human styloid process according to claim 1, characterized in that: The longitudinal moving mechanism is provided with two first fixed seats, a first threaded rod and a first movable seat, the first fixed seats are arranged along both sides of the wide side of the chassis, the first threaded rod is rotatably arranged on the first fixed seat, the first movable seat is slidably arranged on the first fixed seat and is threadedly connected to the first threaded rod, the transverse moving mechanism includes a second fixed seat, a second threaded rod and a second movable seat, the second fixed seat is arranged on the first movable seat, the second movable seat is slidably arranged on the second fixed seat and is threadedly connected to the second threaded rod, and the vertical moving mechanism is fixedly arranged on the second movable seat.

4. The medical experimental staining device for studying excessive elongation of human styloid process according to claim 1, characterized in that: It also includes a sealing mechanism, which includes a cover glass rack arranged on the front inner wall of the chassis and a control box on the rear inner wall, the control box is connected to multiple rotating structures above and a linear drive structure is fixed below, the multiple rotating structures are connected to clamps, and the linear drive structure is connected to a third movable seat for adjusting the opening sizes of multiple clamps, and the cover glass rack is provided with a plurality of inclined support frames, and when sealing is performed, the slide rack moves between the cover glass rack and the control box.

5. The medical experimental staining device for studying excessive elongation of human styloid process according to claim 4, characterized in that: The multiple rotating structures include multiple rotating drivers and multiple driving shafts which are respectively connected to the top wall of the control box through a first connecting column and a second connecting column. The output shaft of the rotating driver is fixed to the driving shaft, and the driving shaft is rotatably connected to the clamp. The multiple driving shaft sleeves are provided with sprockets, and the corresponding two sprockets are connected through the same chain transmission.

6. The medical experimental staining device for studying excessive elongation of human styloid process according to claim 4, characterized in that: A plurality of rotating seats are installed on the third movable seat, and positioning holes are arranged on the rotating seats, and the clamps are inserted into the positioning holes.

7. The medical experimental staining device for studying excessive elongation of human styloid process according to claim 4, characterized in that: The clamp is two elastic clamping pieces with one end fixed together.

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