Whitening composition, whitening essence and preparation method thereof

Abstract

The invention provides a whitening composition, whitening essence and a preparation method thereof, the whitening composition is prepared from the following raw materials in parts by weight: 10 to 15 parts of extract compound, 3 to 5 parts of compound polysaccharide, 2 to 4 parts of galactose yeast-like bacterium fermentation product filtrate, 1 to 3 parts of rhodopseudomonas palustris liquid, 0.1 to 0.3 part of soluble collagen and 0.5 to 1 part of superoxide dismutase; the extract compound is prepared from the following raw materials in parts by weight: 5 to 7 parts of radix cynanchi atrati extract, 3 to 5 parts of vitex agnus linteus extract, 1 to 3 parts of mussel extract, 2 to 4 parts of wild soybean seed extract and 1 to 3 parts of chamomilla recutita flower extract. The components of the whitening composition are reasonably compounded, the whitening composition is mild and non-irritant, synergistic interaction is achieved, rapid first-aid whitening can be achieved, 15-min first-aid whitening can be achieved, the skin can be restored to be bright and white, and the freckle removing and whitening effects can be comprehensively achieved by damaging melanocytes, inhibiting melanin generation and promoting melanin degradation. Meanwhile, the repairing, moisturizing and soothing effects are considered, no irritation is caused, and the safety is high.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a whitening composition, a whitening essence and a preparation method thereof. Background Art

[0002] The skin is the first barrier of the human body and has the most contact with the external environment. The skin, especially the facial skin, is prone to some darker spots or plaques. Endogenous factors (inside and outside the cells) and exogenous factors (ultraviolet radiation) increase melanin and cause excessive pigmentation, resulting in spots and changes in skin color. Endogenous factors are mainly affected by extracellular cytokines, hormones, extracellular matrix and intracellular multi-enzymes, and signal transduction pathways; exogenous factors such as ultraviolet radiation cause melanocyte proliferation, increase tyrosinase activity, and promote melanin production. In recent years, people's pace of life has accelerated, work pressure has increased, life has been irregular, and sleep quality has been poor. Most people are troubled by dull skin and spots.

[0003] At present, there are many whitening and freckle-removing ingredients used in cosmetics, such as kojic acid, arbutin, fruit acid, tranexamic acid, vitamin C and its derivatives, polypeptides, etc. Traditional whitening and freckle-removing products often contain many chemical substances, which can cause certain damage to the skin and easily cause allergic reactions such as acne and redness. In addition, in order to achieve a good freckle-removing and whitening effect, the amount of active ingredients added to whitening products is very high. For people with sensitive skin or slight skin damage, the irritation will also increase accordingly. If the irritation is small, the whitening treatment cycle is too long and consumers will give up halfway. Although some existing products also use plant extracts with higher safety to improve the whitening and freckle-removing effects, the combination of plant extracts is unreasonable, and the freckle-removing and whitening effects are not ideal.

[0004] Therefore, it is necessary to find a whitening composition that is less irritating, highly safe, and has good freckle removal and whitening effects to enhance consumer experience and usage experience. Summary of the invention

[0005] In view of this, the present invention provides a whitening composition, a whitening essence and a preparation method thereof.

[0006] The technical solution of the present invention is achieved in this way:

[0007] The whitening composition comprises the following raw materials in parts by weight: 10-15 parts of an extract compound, 3-5 parts of a complex polysaccharide, 2-4 parts of a filtrate of a galactosyl yeast-like fermentation product, 1-3 parts of a bacterial solution of Rhodopseudomonas palustris, 0.1-0.3 parts of a soluble collagen, and 0.5-1 parts of a superoxide dismutase;

[0008] The extract compound comprises the following raw materials in parts by weight: 5-7 parts of iris sibiricum extract, 3-5 parts of vitex agnus-castus extract, 1-3 parts of mussel extract, 2-4 parts of glycine max seed extract and 1-3 parts of chamomile flower extract.

[0009] Furthermore, the preparation method of the extract compound is specifically as follows:

[0010] (1) crushing the white osmanthus into powder, adding it to water, adding Lactobacillus plantarum and Lactobacillus rhamnosus in a mass ratio of 1:2-4 for fermentation, concentrating the fermentation liquid, mixing the concentrated product with an ethanol solution with a concentration of 50%-60% v / v, filtering, concentrating the filtrate, and vacuum drying to obtain a white osmanthus extract;

[0011] (2) crushing chamomile into powder, adding water, adding Lactobacillus plantarum and Lactobacillus rhamnosus in a mass ratio of 1:2-4 for fermentation, concentrating the fermentation liquid, mixing the concentrated product with an ethanol solution with a concentration of 50%-60% v / v, filtering, concentrating the filtrate, and vacuum drying to obtain a chamomile extract;

[0012] (3) crushing and squeezing wild soybean seeds, subjecting the squeezing product to ultrahigh pressure treatment, centrifuging, collecting the supernatant, flocculating the supernatant, centrifuging again, and fine filtering the supernatant to obtain a wild soybean seed extract;

[0013] (4) grinding and crushing Vitex agnus-castus, sieving through a 40-60 mesh, adding into an ethanol-ammonium sulfate aqueous two-phase system, ultrasonically extracting, concentrating under reduced pressure, and drying to obtain a Vitex agnus-castus extract;

[0014] (5) Mixing the Bletilla striata extract, the Vitex agnus-castus extract, the Mytilus edulis extract, the Glycine max seed extract and the Chamomile flower extract to obtain a target extract compound.

[0015] Furthermore, in step (1), the solid-liquid ratio of the white osmanthus to water is 10-15:1 g / mL, the total mass of the Lactobacillus plantarum and the Lactobacillus rhamnosus is 0.02-0.04 of the mass of the white osmanthus; the fermentation is carried out at a temperature of 20-30° C. for 1-3 days; the solid-liquid ratio of the concentrated product to the ethanol solution is 1:5-8 g / mL;

[0016] In step (2), the solid-liquid ratio of the chamomile flower to water is 10-15:1 g / mL; the total mass of the plant lactobacillus and the rhamnosus lactobacillus is 0.02-0.04 of the mass of the chamomile flower; the fermentation is carried out at a temperature of 20-30° C. for 1-3 days; the solid-liquid ratio of the concentrated product to the ethanol solution is 1:5-8 g / mL;

[0017] In step (3), the ultrahigh pressure treatment is carried out at 100-200 MPa for 6-10 min; the flocculating flocculant is a xanthan gum and acrylamide-cationic guar gum graft copolymer in a mass ratio of 1:1-3;

[0018] The pore size of the fine filter is 1-2 μm;

[0019] In step (4), the solid-liquid ratio of the Vitex agnus-castus to the ethanol-ammonium sulfate aqueous two-phase system is 1:8-10 g / mL; the concentration of the ethanol-ammonium sulfate aqueous two-phase system is 30%-50% v / v, wherein the volume of ethanol to ammonium sulfate is 1-3:1; the rotation speed of the ultrasonic extraction is 400-500 rpm, the temperature is 70-80° C., and the time is 60-90 min.

[0020] Furthermore, the composite polysaccharide is a polysaccharide of Porphyra yezoensis, a polysaccharide of Dendrobium officinale and a polysaccharide of Pueraria lobata in a mass ratio of 3-5:2-4:1.

[0021] The preparation method of the whitening composition comprises the following specific steps: uniformly mixing the extract compound, complex polysaccharide, galactosaccharide yeast-like bacteria fermentation product filtrate, Rhodopseudomonas palustris bacterial liquid, soluble collagen and superoxide dismutase to obtain the whitening composition.

[0022] The whitening essence comprises the whitening composition and auxiliary materials.

[0023] Furthermore, the content of the whitening composition in the whitening essence is 1-10wt%.

[0024] Furthermore, the auxiliary materials include the following raw materials in parts by weight: 1-2 parts of 1,3-butanediol, 0.5-0.8 parts of triethanolamine, 0.05-0.08 parts of p-hydroxyacetophenone, 0.01-0.03 parts of disodium ethylenediaminetetraacetate, and 50-70 parts of water.

[0025] The preparation method of the whitening essence comprises the following specific steps:

[0026] (1) 1,3-butanediol, triethanolamine, p-hydroxyacetophenone and disodium ethylenediaminetetraacetate are uniformly mixed to obtain a premix;

[0027] (2) adding the premix to water and performing ultrasonic treatment to obtain a mixed solution;

[0028] (3) adding the whitening composition to the mixed solution, homogenizing, filtering, and sterilizing to obtain a whitening essence.

[0029] Furthermore, in step (2), the ultrasonic treatment is carried out at 50-80kHz and 50-60°C for 30-40min; in step (3), the homogenization is carried out at a temperature of 55-65°C, first stirring at 2000-4000rpm for 8-10min, and then continuing to stir at 1000-2000rpm for 10-15min.

[0030] Compared with the prior art, the present invention has the following beneficial effects:

[0031] 1. The whitening composition of the present invention (extract compound, complex polysaccharide, galactosyl yeast fermentation product filtrate, palustris red pseudomonas liquid) is reasonably compounded, mild and non-irritating, synergistic, and can quickly whiten the skin, and can achieve 15 minutes of emergency whitening, restore the skin to a bright white, and achieve a comprehensive and comprehensive freckle removal and whitening effect by destroying melanocytes, inhibiting melanin production, and promoting melanin degradation. At the same time, it takes into account the repair, moisturizing, and soothing effects, is non-irritating, and has high safety.

[0032] 2. The extract compound of the present invention uses a specific extraction method to obtain a substance with higher activity, better purity, and higher yield, which fully guarantees the stability of the effective active ingredients and can better coordinate whitening, repairing, moisturizing and soothing. DETAILED DESCRIPTION

[0033] In order to better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention.

[0034] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0035] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial sources.

[0036] The mussel extract of the present invention was purchased from Xi'an Denohaisi Medical Technology Co., Ltd.

[0037] Example 1

[0038] The whitening composition of this embodiment comprises the following raw materials in parts by weight: 13 parts of an extract compound, 4 parts of complex polysaccharides (porphyra yezoensis polysaccharide, dendrobium polysaccharide and kudzu root polysaccharide in a mass ratio of 4:3:1), 3 parts of galactosaccharomyces fermentation product filtrate, 2 parts of Rhododendron palustris bacterial liquid, 0.2 parts of soluble collagen, and 0.8 parts of superoxide dismutase; wherein the extract compound comprises the following raw materials in parts by weight: 6 parts of white osmanthus extract, 4 parts of vitex agnus-castus extract, 2 parts of mussel extract, 3 parts of wild soybean seed extract and 2 parts of chamomile flower extract.

[0039] The preparation method of the above extract compound is specifically as follows:

[0040] (1) Grinding the white osmanthus into powder, adding it to water at a solid-liquid ratio of 13:1 g / mL, adding 0.03 of the mass of white osmanthus with Lactobacillus plantarum and Lactobacillus rhamnosus (the mass ratio of Lactobacillus plantarum and Lactobacillus rhamnosus is 1:3), fermenting at a temperature of 25° C. for 2 days, concentrating the fermentation liquid, and mixing the concentrated product with an ethanol solution with a concentration of 55% v / v at a solid-liquid ratio of 1:7 g / mL, filtering, concentrating the filtrate, and vacuum drying to obtain a white osmanthus extract;

[0041] (2) Crush the chamomile flower into powder, add it to water at a solid-liquid ratio of 13:1 g / mL, add 0.03 of the mass of chamomile flower with Lactobacillus plantarum and Lactobacillus rhamnosus (the mass ratio of Lactobacillus plantarum and Lactobacillus rhamnosus is 1:3), ferment at 25° C. for 2 days, concentrate the fermentation liquid, and mix the concentrated product with an ethanol solution with a concentration of 55% v / v at a solid-liquid ratio of 1:7 g / mL, filter, concentrate the filtrate, and vacuum dry to obtain a chamomile flower extract;

[0042] (3) The wild soybean seeds were crushed and pressed, and the pressed product was subjected to ultrahigh pressure treatment at 150 MPa for 8 min, centrifuged, and the supernatant was collected. The supernatant was flocculated using a xanthan gum and acrylamide-cationic guar gum graft copolymer with a mass ratio of 1:2 as a flocculant, and the supernatant was centrifuged twice, and the supernatant was finely filtered at a pore size of 1.5 μm to obtain the wild soybean seed extract;

[0043] (4) grinding and crushing Vitex agnus-castus, sieving through 50 mesh, adding into 40% v / v ethanol-ammonium sulfate aqueous two-phase system (volume of ethanol and ammonium sulfate is 2:1) at a solid-liquid ratio of 1:9 g / mL, extracting by ultrasound (speed of 450 rpm, temperature of 75° C.) for 75 min, concentrating under reduced pressure, and drying to obtain Vitex agnus-castus extract;

[0044] (5) Mixing the Bletilla striata extract, the Vitex agnus-castus extract, the Mytilus edulis extract, the Glycine max seed extract and the Chamomile flower extract to obtain a target extract compound.

[0045] The preparation method of the whitening composition comprises the following specific steps: uniformly mixing the extract compound, complex polysaccharide, galactosaccharide yeast-like bacteria fermentation product filtrate, Rhodopseudomonas palustris bacterial liquid, soluble collagen and superoxide dismutase to obtain the whitening composition.

[0046] The whitening essence of this embodiment comprises the above-mentioned whitening composition (content of 5wt%) and auxiliary materials, wherein the auxiliary materials include the following raw materials in parts by weight: 1.5 parts of 1,3-butylene glycol, 0.7 parts of triethanolamine, 0.07 parts of p-hydroxyacetophenone, 0.02 parts of disodium ethylenediaminetetraacetate, and 60 parts of water.

[0047] The preparation method of the above whitening essence comprises the following specific steps:

[0048] (1) 1,3-butanediol, triethanolamine, p-hydroxyacetophenone and disodium ethylenediaminetetraacetate are uniformly mixed to obtain a premix;

[0049] (2) adding the premix to water and treating with ultrasound (65 kHz, 55° C.) for 35 min to obtain a mixed solution;

[0050] (3) Add the whitening composition to the mixed solution, homogenize (at 60° C., first stir at 3000 rpm for 9 min, then continue stirring at 1500 rpm for 13 min), filter, and sterilize to obtain a whitening essence.

[0051] Example 2

[0052] The whitening composition of this embodiment comprises the following raw materials in parts by weight: 10 parts of an extract compound, 3 parts of complex polysaccharides (porphyra yezoensis polysaccharide, dendrobium polysaccharide and kudzu root polysaccharide in a mass ratio of 3:2:1), 2 parts of galactosaccharomyces fermentation product filtrate, 1 part of Rhodopseudomonas palustris bacterial liquid, 0.1 part of soluble collagen, and 0.5 part of superoxide dismutase; wherein the extract compound comprises the following raw materials in parts by weight: 5 parts of white osmanthus extract, 3 parts of vitex agnus-castus extract, 1 part of mussel extract, 2 parts of soybean seed extract and 1 part of chamomile flower extract.

[0053] The preparation method of the above extract compound is specifically as follows:

[0054] (1) crushing the white mulberry into powder, adding it to water at a solid-liquid ratio of 10:1 g / mL, adding 0.02 of the mass of the white mulberry with Lactobacillus plantarum and Lactobacillus rhamnosus (the mass ratio of Lactobacillus plantarum and Lactobacillus rhamnosus is 1:2), fermenting at a temperature of 20° C. for 1 day, concentrating the fermentation liquid, and mixing the concentrated product with an ethanol solution with a concentration of 50% v / v at a solid-liquid ratio of 1:5 g / mL, filtering, concentrating the filtrate, and vacuum drying to obtain a white mulberry extract;

[0055] (2) Crush the chamomile into powder, add it to water at a solid-liquid ratio of 10:1 g / mL, add 0.02 of the mass of the chamomile by weight of Lactobacillus plantarum and Lactobacillus rhamnosus (the mass ratio of Lactobacillus plantarum and Lactobacillus rhamnosus is 1:2), ferment at a temperature of 20° C. for 1 day, concentrate the fermentation liquid, and at a solid-liquid ratio of 1:5 g / mL, mix the concentrated product with an ethanol solution with a concentration of 50% v / v, filter, concentrate the filtrate, and vacuum dry to obtain a chamomile extract;

[0056] (3) The wild soybean seeds were crushed and pressed, and the pressed product was subjected to ultrahigh pressure treatment at 100 MPa for 6 min, centrifuged, and the supernatant was collected. The supernatant was flocculated using a xanthan gum and acrylamide-cationic guar gum graft copolymer with a mass ratio of 1:1 as a flocculant, and the supernatant was centrifuged twice, and the supernatant was finely filtered at a pore size of 1 μm to obtain the wild soybean seed extract;

[0057] (4) grinding and crushing Vitex agnus-castus, sieving through 40 mesh, adding into a 30% v / v ethanol-ammonium sulfate aqueous two-phase system (the volume of ethanol and ammonium sulfate is 1:1) at a solid-liquid ratio of 1:8 g / mL, extracting by ultrasound (speed of 400 rpm, temperature of 70°C) for 60 min, concentrating under reduced pressure, and drying to obtain a Vitex agnus-castus extract;

[0058] (5) Mixing the Bletilla striata extract, the Vitex agnus-castus extract, the Mytilus edulis extract, the Glycine max seed extract and the Chamomile flower extract to obtain a target extract compound.

[0059] The preparation method of the whitening composition comprises the following specific steps: uniformly mixing the extract compound, complex polysaccharide, galactosaccharide yeast-like bacteria fermentation product filtrate, Rhodopseudomonas palustris bacterial liquid, soluble collagen and superoxide dismutase to obtain the whitening composition.

[0060] The whitening essence of this embodiment comprises the above-mentioned whitening composition (content is 1wt%) and auxiliary materials, wherein the auxiliary materials include the following raw materials in parts by weight: 1 part of 1,3-butylene glycol, 0.5 parts of triethanolamine, 0.05 parts of p-hydroxyacetophenone, 0.01 parts of disodium ethylenediaminetetraacetate, and 50 parts of water.

[0061] The preparation method of the above whitening essence comprises the following specific steps:

[0062] (1) 1,3-butanediol, triethanolamine, p-hydroxyacetophenone and disodium ethylenediaminetetraacetate are uniformly mixed to obtain a premix;

[0063] (2) adding the premix to water and treating with ultrasound (50 kHz, 50° C.) for 30 min to obtain a mixed solution;

[0064] (3) Add the whitening composition to the mixed solution, homogenize (at a temperature of 55° C., first stir at 2000 rpm for 8 min, then continue stirring at 1000 rpm for 10 min), filter, and sterilize to obtain a whitening essence.

[0065] Example 3

[0066] The whitening composition of this embodiment comprises the following raw materials in parts by weight: 15 parts of an extract compound, 5 parts of complex polysaccharides (porphyra yezoensis polysaccharide, dendrobium polysaccharide and kudzu root polysaccharide in a mass ratio of 5:4:1), 4 parts of galactosaccharomyces fermentation product filtrate, 3 parts of Rhodopseudomonas palustris bacterial liquid, 0.3 parts of soluble collagen, and 1 part of superoxide dismutase; wherein the extract compound comprises the following raw materials in parts by weight: 7 parts of white osmanthus extract, 5 parts of vitex agnus-castus extract, 3 parts of mussel extract, 4 parts of glycine max seed extract and 3 parts of chamomile flower extract.

[0067] The preparation method of the above extract compound is specifically as follows:

[0068] (1) Grinding the white osmanthus into powder, adding it to water at a solid-liquid ratio of 15:1 g / mL, adding 0.04 of the mass of white osmanthus with Lactobacillus plantarum and Lactobacillus rhamnosus (the mass ratio of Lactobacillus plantarum and Lactobacillus rhamnosus is 1:4), fermenting at a temperature of 30° C. for 3 days, concentrating the fermentation liquid, and mixing the concentrated product with an ethanol solution with a concentration of 60% v / v at a solid-liquid ratio of 1:8 g / mL, filtering, concentrating the filtrate, and vacuum drying to obtain a white osmanthus extract;

[0069] (2) Crush the chamomile flower into powder, add it to water at a solid-liquid ratio of 15:1 g / mL, add 0.04 of the mass of chamomile flower with Lactobacillus plantarum and Lactobacillus rhamnosus (the mass ratio of Lactobacillus plantarum and Lactobacillus rhamnosus is 1:4), ferment at 30° C. for 3 days, concentrate the fermentation liquid, and mix the concentrated product with an ethanol solution with a concentration of 60% v / v at a solid-liquid ratio of 1:8 g / mL, filter, concentrate the filtrate, and vacuum dry to obtain a chamomile flower extract;

[0070] (3) The wild soybean seeds were crushed and pressed, and the pressed product was subjected to ultrahigh pressure treatment at 200 MPa for 10 min, centrifuged, and the supernatant was collected. The supernatant was flocculated using a xanthan gum and acrylamide-cationic guar gum graft copolymer with a mass ratio of 1:3 as a flocculant, and the supernatant was centrifuged twice, and the supernatant was finely filtered at a pore size of 2 μm to obtain the wild soybean seed extract;

[0071] (4) grinding and crushing Vitex agnus-castus, sieving through 60 mesh, adding into a 50% v / v ethanol-ammonium sulfate aqueous two-phase system (the volume of ethanol and ammonium sulfate is 3:1) at a solid-liquid ratio of 1:10 g / mL, extracting by ultrasound (speed of 500 rpm, temperature of 80° C.) for 90 min, concentrating under reduced pressure, and drying to obtain a Vitex agnus-castus extract;

[0072] (5) Mixing the Bletilla striata extract, the Vitex agnus-castus extract, the Mytilus edulis extract, the Glycine max seed extract and the Chamomile flower extract to obtain a target extract compound.

[0073] The preparation method of the whitening composition comprises the following specific steps: uniformly mixing the extract compound, complex polysaccharide, galactosaccharide yeast-like bacteria fermentation product filtrate, Rhodopseudomonas palustris bacterial liquid, soluble collagen and superoxide dismutase to obtain the whitening composition.

[0074] The whitening essence of this embodiment comprises the above-mentioned whitening composition (content of 10 wt%) and auxiliary materials, wherein the auxiliary materials include the following raw materials in parts by weight: 2 parts of 1,3-butylene glycol, 0.8 parts of triethanolamine, 0.08 parts of p-hydroxyacetophenone, 0.03 parts of disodium ethylenediaminetetraacetate, and 70 parts of water.

[0075] The preparation method of the above whitening essence comprises the following specific steps:

[0076] (1) 1,3-butanediol, triethanolamine, p-hydroxyacetophenone and disodium ethylenediaminetetraacetate are uniformly mixed to obtain a premix;

[0077] (2) adding the premix to water and treating with ultrasound (80 kHz, 60° C.) for 40 min to obtain a mixed solution;

[0078] (3) Add the whitening composition to the mixed solution, homogenize (at 65° C., first stir at 4000 rpm for 10 min, then continue stirring at 2000 rpm for 15 min), filter, and sterilize to obtain a whitening essence.

[0079] Comparative Example 1

[0080] The difference from Example 1 is that the amount of raw materials used in the whitening composition is different, and the rest is consistent with Example 1.

[0081] The whitening composition of this comparative example comprises the following raw materials in parts by weight: 8 parts of an extract compound, 6 parts of complex polysaccharides, 5 parts of galactosyl yeast-like bacteria fermentation product filtrate, 1 part of Rhodopseudomonas palustris bacterial liquid, 0.4 parts of soluble collagen, and 0.4 parts of superoxide dismutase.

[0082] Comparative Example 2

[0083] The difference from Example 1 is that the extract compound lacks the Bletilla striata extract, and the missing component is allocated to other components according to the proportion. The rest is consistent with Example 1.

[0084] Comparative Example 3

[0085] The difference from Example 1 is that the complex polysaccharide lacks Porphyra yezoensis polysaccharide, and the missing component is distributed to other components according to the proportion. The rest is consistent with Example 1.

[0086] Comparative Example 4

[0087] The difference from Example 1 is that the raw material of the whitening composition lacks the filtrate of the fermentation product of galactosyl yeast-like bacteria, and the missing component is allocated to other components according to the proportion. The rest is consistent with Example 1.

[0088] Comparative Example 5

[0089] The difference from Example 1 is that the raw material of the whitening composition lacks the bacterial solution of Rhodopseudomonas palustris, and the missing component is allocated to other components according to the proportion. The rest is consistent with Example 1.

[0090] Comparative Example 6

[0091] The difference from Example 1 is that the preparation method of the extract compound is conventional water extraction, and the rest is consistent with Example 1.

[0092] That is, the preparation method of the extract compound of this comparative example is specifically as follows:

[0093] (1) crushing the white osmanthus fragrans, adding it to water at a solid-liquid ratio of 1:13 g / mL, extracting it at 60° C. for 6 h, collecting the extract, concentrating and drying it to obtain a white osmanthus fragrans extract;

[0094] (2) crushing Vitex agnus-castus, adding the mixture into water at a solid-liquid ratio of 1:13 g / mL, extracting the mixture at 60° C. for 6 h, collecting the extract, concentrating and drying the extract, and obtaining a Vitex agnus-castus extract;

[0095] (3) Glycine max seeds were crushed, added into water at a solid-liquid ratio of 1:13 g / mL, extracted at 60° C. for 6 h, and the extract was collected, concentrated and dried to obtain a glycine max seed extract;

[0096] (4) crushing the chamomile flower, adding it to water at a solid-liquid ratio of 1:13 g / mL, extracting it at 60° C. for 6 h, collecting the extract, concentrating and drying it to obtain a chamomile flower extract;

[0097] (5) Mixing the Bletilla striata extract, the Vitex agnus-castus extract, the Mytilus edulis extract, the Glycine max seed extract and the Chamomile flower extract to obtain a target extract compound.

[0098] Experimental situation

[0099] 1. Efficacy evaluation of in vitro melanin epidermal model

[0100] Experimental principle: Skinovo-Mela is an epidermal model containing melanocytes that is reconstructed in vitro by 3D printing using human keratinocytes and melanocytes as seed cells. The structure of this model is highly similar to human skin, with melanin particle distribution and a stratified epidermal structure. Using the epidermal model containing melanocytes, Skinovo-Mela is used to test relevant indicators to evaluate whether the sample to be tested has the corresponding efficacy.

[0101] Experimental method: add the pre-warmed human in vitro recombinant melanin model culture medium to a 6-well culture plate (1 mL / well), transfer the epidermal model containing melanocytes to the 6-well plate containing the culture medium, and place it in an incubator for overnight culture. Divide into sample groups (Examples 1-3 and Comparative Examples 1-6), negative control group (PBS), positive control group (kojic acid), take 25 μL of the test sample, evenly smear the model surface, set 3 parallels for each test group, expose for 1 hour, thoroughly rinse the sample with DPBS until there is no residue, and then place it in a six-well plate containing fresh maintenance culture medium and continue incubation for 48 hours. Use tweezers to carefully remove the model from the well plate, use a sharp blade to cut the tissue, and measure the L value and melanin particle distribution (use Skin-ColorimeterCL400 to measure the L value, and the results are shown in Table 1. The removed experimental tissue was fixed in 4% paraformaldehyde solution, fully dehydrated with 30% sucrose solution, and finally embedded with OCT frozen section embedding agent. During the fixation process, it is necessary to pay attention to eliminate the bubbles in the OCT glue, and then quickly placed in liquid nitrogen, marked the group and transferred to -80℃ for storage. After the tissue section is stained, it is stained with a melanin staining kit, referring to the instructions of the Shangbao Biological Melanin Staining Kit, and the results are shown in Table 2).

[0102] The data are expressed as mean ± standard deviation and analyzed using SPSS. The differences were considered statistically significant if p < 0.05.

[0103] Table 1

[0104]

[0105]

[0106] *: Compared with the negative control group, the difference is statistically significant (p<0.05)

[0107] As can be seen from Table 1, the whitening essence prepared in Examples 1-3 of the present invention has a significantly improved L value compared with the negative control group (p<0.05), and exceeds the effect of the positive control group (p<0.05). Although the L value of Comparative Examples 1-6 is improved relative to the negative control group, the effect is lower than that of Examples 1-3. This shows that the various components of the present invention are reasonably compounded, synergistically enhanced, and indispensable, and can improve the L value of the epidermal model containing melanocytes and have a whitening effect.

[0108] Table 2

[0109] sample Melanin particle ratio Negative control group 100.00±14.83 Positive control group 35.20±11.05* Example 1 67.18±9.51* Example 2 67.07±10.77* Example 3 67.13±11.01* Comparative Example 1 88.38±9.88* Comparative Example 2 93.77±10.68* Comparative Example 3 90.37±9.44* Comparative Example 4 91.54±10.14* Comparative Example 5 92.66±11.21* Comparative Example 6 89.34±11.33*

[0110] *: Compared with the negative control group, the difference is statistically significant (p<0.05)

[0111] As can be seen from Table 2, the whitening essence prepared in Examples 1-3 of the present invention has a significantly lower percentage of melanin granule content than the negative control group (p<0.05), wherein the percentage of melanin granule content in Example 1 is reduced by 32.82%. Although the percentage of melanin granule content in Comparative Examples 1-6 is lower than that in the negative control group, the effect is lower than that in Examples 1-3. This shows that the various components of the present invention are reasonably compounded, synergistically enhanced, and indispensable, and can reduce the content of melanin granules in the epidermal model containing melanocytes, and has a whitening effect.

[0112] 2. Irritation test

[0113] With reference to the Technical Specifications for Safety of Cosmetics (2015 edition), a closed patch test was performed behind the ear of the test subject. 0.02 mL of the test substance was dropped into the patch tester, which was then sealed behind the left ear of the test subject with tape. After 30 minutes, the test substance patch tester was removed, the skin reaction was observed, and the score was recorded.

[0114] Among them, skin reaction is a negative reaction, with a score of 0;

[0115] The skin reaction is a suspicious reaction with only slight erythema, and the score is 1;

[0116] Skin reactions include erythema, infiltration, edema, and papules, with a score of 2;

[0117] Skin reactions included erythema, infiltration, edema, and herpes, with a score of 3;

[0118] Skin reactions included obvious erythema, severe infiltration, edema, and confluent herpes, with a score of 4.

[0119] The test results of each group were averaged, and the specific results are shown in Table 3.

[0120] Table 3

[0121] sample Rating result Examples 1-3 0 No stimulation

[0122] As can be seen from Table 5, the whitening compositions prepared in Examples 1-3 of the present invention are mild, non-irritating and harmless to the skin, suitable for use by people with sensitive skin, and can enhance the user's experience of use.

[0123] 3. Clinical Data

[0124] (1) Immediate efficacy test

[0125] Test sample: whitening essence prepared in Example 1;

[0126] Test subjects: 30 subjects were selected, aged 18-50 years old, 15 males and 15 females, with no restrictions on skin type;

[0127] How to use: After cleansing and toning in the morning and evening, apply an appropriate amount of this product evenly on the face and pat and massage until absorbed for 15 minutes;

[0128] Evaluation indicators:

[0129]

[0130] Scoring criteria: 1 for strongly disagree, 2 for somewhat disagree, 3 for basically disagree, 4 for basically agree, 5 for somewhat agree, 6 for strongly agree;

[0131] Formula for calculating the degree of recognition: Recognition (%) = (number of people who scored 4 points + number of people who scored 5 points + number of people who scored 6 points) / total number of participants × 100%

[0132] The binomial distribution method was used for statistical analysis, and the expected value of recognition was set at 60% (the proportion of people with 4 points + 5 points + 6 points). P < 0.05 indicated a significant difference.

[0133] The results are shown in Table 4.

[0134] Table 4

[0135]

[0136]

[0137] As can be seen from Table 4, the whitening essence prepared in Example 1 of the present invention can achieve rapid whitening in 15 minutes, and has repairing, moisturizing and soothing effects, and is recognized by users.

[0138] (2) Long-term effect test

[0139] Experimental subjects: 30 volunteers, 15 males and 15 females, aged 18-50 years old, with sensitive skin, accompanied by sensitive symptoms such as redness and red blood streaks.

[0140] Grouping and use method: Volunteers used the whitening essence prepared in Example 1 of the present invention, applied it on the face once in the morning and once in the evening, and used it continuously for 28 days;

[0141] Test indicators and standards: Instrumental testing of facial skin indicators was performed at 0d, 14d and 28d;

[0142] Water content of the stratum corneum of the skin: The moisture content of the stratum corneum of the skin at each test time point was measured using the probe CorneometerB CM 825;

[0143] Transepidermal water loss value: The probe TewameterR TM Hex was used to measure the transepidermal water loss value TEWL [g / (hem 2 )];

[0144] Skin melanin: Use the probe MexameterQ MX18 to measure the skin melanin at each test time point;

[0145] Skin L* value, b* value, ITA° value: The skin image acquisition instrument VISIA-CR was used to acquire images, and the analysis system Image-Pro Plus was used to analyze the L* value, b* value, and ITA° value.

[0146] SPSS was used for statistical analysis of the data. The test data were tested for normal distribution. The asymptotic significance of the normality test (bilateral) was P>0.05. The measurement data were expressed as mean±standard deviation, and the significance level was P<0.05.

[0147] The test results are shown in Table 5.

[0148] Table 5

[0149]

[0150]

[0151] *: P<0.05 compared with 0d

[0152] As can be seen from Table 5, after the whitening essence prepared in Example 1 of the present invention was used for 14d and 28d, the moisture content of the skin stratum corneum in the test area of ​​the subject increased by 20.12% and 34.34% respectively, with significant differences. The transepidermal water loss values ​​in the test area of ​​the subject decreased by 26.91% and 37.23% at 14d and 28d, respectively, with significant differences. The lactic acid stinging test scores in the test area of ​​the subject decreased by 10.08% and 11.84% at 14d and 28d, respectively, with significant differences. The skin melanin in the test area of ​​the subject decreased by 8.62% and 17.89% after 14d and 28d, respectively, with significant differences. The skin brightness L* value in the test area of ​​the subject increased by 1.78% and 3.13% after 14d and 28d, respectively, with significant differences. The skin yellow-blue b* value in the test area of ​​the subject decreased by 1.69% and 3.38% after 14d and 28d, respectively, with significant differences. The skin individual type angle ITA° values ​​of the test area of ​​the subjects increased by 8.76% and 15.51% after 14d and 28d, respectively, which was a significant difference.

[0153] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A whitening composition, characterized in that: The raw materials include the following in parts by weight: 10-15 parts of extract compound, 3-5 parts of complex polysaccharide, 2-4 parts of galactosyl yeast-like bacteria fermentation product filtrate, 1-3 parts of Rhodopseudomonas palustris bacterial liquid, 0.1-0.3 parts of soluble collagen, and 0.5-1 parts of superoxide dismutase; The extract compound comprises the following raw materials in parts by weight: 5-7 parts of iris sibiricum extract, 3-5 parts of vitex agnus-castus extract, 1-3 parts of mussel extract, 2-4 parts of glycine max seed extract and 1-3 parts of chamomile flower extract.

2. The whitening composition according to claim 1, characterized in that The preparation method of the extract compound is specifically as follows: (1) crushing the white osmanthus into powder, adding it to water, adding Lactobacillus plantarum and Lactobacillus rhamnosus in a mass ratio of 1:2-4 for fermentation, concentrating the fermentation liquid, mixing the concentrated product with an ethanol solution with a concentration of 50%-60% v / v, filtering, concentrating the filtrate, and vacuum drying to obtain a white osmanthus extract; (2) crushing chamomile into powder, adding water, adding Lactobacillus plantarum and Lactobacillus rhamnosus in a mass ratio of 1:2-4 for fermentation, concentrating the fermentation liquid, mixing the concentrated product with an ethanol solution with a concentration of 50%-60% v / v, filtering, concentrating the filtrate, and vacuum drying to obtain a chamomile extract; (3) crushing and squeezing wild soybean seeds, subjecting the squeezing product to ultrahigh pressure treatment, centrifuging, collecting the supernatant, flocculating the supernatant, centrifuging again, and fine filtering the supernatant to obtain a wild soybean seed extract; (4) grinding and crushing Vitex agnus-castus, sieving through a 40-60 mesh, adding into an ethanol-ammonium sulfate aqueous two-phase system, ultrasonically extracting, concentrating under reduced pressure, and drying to obtain a Vitex agnus-castus extract; (5) Mixing the Bletilla striata extract, the Vitex agnus-castus extract, the Mytilus edulis extract, the Glycine max seed extract and the Chamomile flower extract to obtain a target extract compound.

3. The whitening composition according to claim 2, characterized in that In step (1), the solid-liquid ratio of the white mulberry to water is 10-15:1 g / mL, the total mass of the plant lactobacillus and the rhamnosus lactobacillus is 0.02-0.04 of the mass of the white mulberry; the fermentation is carried out at a temperature of 20-30° C. for 1-3 days; the solid-liquid ratio of the concentrated product to the ethanol solution is 1:5-8 g / mL; In step (2), the solid-liquid ratio of the chamomile flower to water is 10-15:1 g / mL; the total mass of the plant lactobacillus and the rhamnosus lactobacillus is 0.02-0.04 of the mass of the chamomile flower; the fermentation is carried out at a temperature of 20-30° C. for 1-3 days; the solid-liquid ratio of the concentrated product to the ethanol solution is 1:5-8 g / mL; In step (3), the ultrahigh pressure treatment is carried out at 100-200 MPa for 6-10 min; the flocculation flocculant is a xanthan gum and acrylamide-cationic guar gum graft copolymer in a mass ratio of 1:1-3; the pore size of the fine filtration is 1-2 μm; In step (4), the solid-liquid ratio of the Vitex agnus-castus to the ethanol-ammonium sulfate aqueous two-phase system is 1:8-10 g / mL; the concentration of the ethanol-ammonium sulfate aqueous two-phase system is 30%-50% v / v, wherein the volume of ethanol to ammonium sulfate is 1-3:1; the rotation speed of the ultrasonic extraction is 400-500 rpm, the temperature is 70-80° C., and the time is 60-90 min.

4. The whitening composition according to claim 1, characterized in that The complex polysaccharide is composed of Porphyra yezoensis polysaccharide, Dendrobium officinale polysaccharide and Pueraria lobata polysaccharide in a mass ratio of 3-5:2-4:

1.

5. The method for preparing the whitening composition according to claim 1, characterized in that: The specific steps include: uniformly mixing the extract compound, complex polysaccharide, galactose yeast-like bacteria fermentation product filtrate, palustris red pseudomonas bacterial liquid, soluble collagen and superoxide dismutase to obtain a whitening composition.

6. Whitening essence, characterized in that, The invention comprises the whitening composition according to claim 1 and auxiliary materials.

7. The whitening essence according to claim 6, characterized in that: The content of the whitening composition in the whitening essence is 1-10wt%.

8. The whitening essence according to claim 6, characterized in that: The auxiliary materials include the following raw materials in parts by weight: 1-2 parts of 1,3-butanediol, 0.5-0.8 parts of triethanolamine, 0.05-0.08 parts of p-hydroxyacetophenone, 0.01-0.03 parts of disodium ethylenediaminetetraacetate, and 50-70 parts of water.

9. The method for preparing the whitening essence according to claim 6, characterized in that: The specific steps include: (1) 1,3-butanediol, triethanolamine, p-hydroxyacetophenone and disodium ethylenediaminetetraacetate are uniformly mixed to obtain a premix; (2) adding the premix to water and performing ultrasonic treatment to obtain a mixed solution; (3) adding the whitening composition to the mixed solution, homogenizing, filtering, and sterilizing to obtain a whitening essence.

10. The method for preparing the whitening essence according to claim 9, characterized in that: In step (2), the ultrasonic treatment is carried out at 50-80kHz and 50-60°C for 30-40min; in step (3), the homogenization is carried out at a temperature of 55-65°C, first stirring at 2000-4000rpm for 8-10min, and then stirring at 1000-2000rpm for 10-15min.

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