Preparation method of gold nano zeolite imidazole framework-8 camptothecin derivative preparation

Abstract

The invention discloses a preparation method of a gold nano zeolite imidazole frame-8 camptothecin derivative preparation, which comprises the following steps: dissolving a chloroauric acid solution in distilled water, and adding sodium citrate to obtain a gold nano solution; adjusting the pH value of the gold nano solution, adding thiohydracrylic acid, stirring, and adding morpholine ethanesulfonic acid to adjust the pH value of the solution; dissolving one of camptothecin, 10-hydroxycamptothecin and irinotecan hydrochloride in water, and adding the solution into the prepared gold nanoparticle solution to obtain a gold nanoparticle-camptothecin derivative solution; dissolving anhydrous zinc acetate and dimethylimidazole in methanol, mixing the anhydrous zinc acetate and the dimethylimidazole, reacting, and centrifuging to obtain zeolite imidazole frame-8 nanoparticles; and completely adding the tannic acid solution into the zeolite imidazole frame-8 nanoparticles, and adding the gold nanoparticle-camptothecin derivative solution to obtain the gold nanoparticle zeolite imidazole frame-8 camptothecin derivative preparation. According to the preparation method of the gold nano zeolite imidazole frame-8 camptothecin derivative preparation, gold nano and zeolite imidazole frame-8 are jointly used as carriers, the drug loading rate of camptothecin derivatives is effectively increased, and the chemotherapy and photo-thermal synergistic treatment effect is effectively improved.

Description

Technical Field

[0001] The invention relates to the field of preparation of anti-tumor drug carrier systems, in particular to a method for preparing a gold nano-zeolite imidazole framework-8 camptothecin derivative preparation. Background Art

[0002] In recent years, a variety of camptothecin derivatives have been used in clinical tumor treatment and have shown efficient anti-tumor effects. Camptothecin derivatives have the problems of large toxic side effects and low bioavailability. How to reduce toxicity and increase water solubility has become a hot research issue. In addition, the nano drug delivery system currently used also has serious problems such as small drug loading, inability to accurately release in cancer cells, and poor targeting.

[0003] Gold nanoparticles have the advantages of good chemical stability, good photothermal effect at a specific nanoscale, and a long blood circulation cycle. For drug delivery systems, they can effectively reduce drug toxicity, and can be used in synergistic treatment with photothermal therapy and chemotherapy to prolong their release time in the blood. They are a good inorganic carrier for drug systems.

[0004] Zeolite imidazole framework material is an organic-inorganic hybrid material, which has been applied in various fields such as catalysis, sensing, bioimaging, and drug delivery. Among them, zeolite imidazole framework-8 nanoparticles have good biocompatibility and pH sensitivity, providing great potential for drug delivery. At present, there are few studies on zeolite imidazole framework-8 nanoparticles loaded with camptothecin and gold nanoparticles at the same time. Therefore, the development of nano drug delivery systems with pH and near-infrared response, good biocompatibility, and effective loading of camptothecin derivatives has broad application prospects. Summary of the invention

[0005] The purpose of the present invention is to provide a method for preparing a gold nano-zeolite imidazole framework-8 camptothecin derivative preparation to solve the problems raised in the above background technology.

[0006] To achieve the above object, the present invention provides the following technical solution: a method for preparing a gold nano-zeolite imidazole framework-8 camptothecin derivative preparation, characterized in that the preparation method comprises the following steps:

[0007] S1: Dissolve 0.31 mL of chloroauric acid solution in 80 mL of distilled water, heat to boiling for ten minutes at 25°C in the dark, then add 0.20 g of sodium citrate to the solution, heat to boiling for 30 minutes, and let stand for 12 hours to obtain a gold nanoparticle solution;

[0008] S2: 0.0735 g of 3-mercaptopropionic acid solution and 0.2262 g of sodium hydroxide were dissolved in 100 mL of water respectively, the gold nanoparticle solution prepared in the above step S1 was adjusted to pH = 11, and then 6 mL of mercaptopropionic acid was added, and stirred for 2 h. The solution prepared by stirring was taken, 50 mmol of morpholineethanesulfonic acid was added to adjust the solution pH = 6.5, 0.016 g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDC) was added, 0.02 g of N-hydroxysuccinimide (NHS) was added, and the reaction was stirred for 30 minutes, and then 0.0417 g of polyethylene glycol (PEG) was added, and the mixture was stirred at room temperature for 24 hours to obtain the prepared gold nanoparticle solution;

[0009] S3: Dissolve 0.0264 g of 10-hydroxycamptothecin in 10 mL of water, dissolve 1.6 g of sodium hydroxide in 10 mL of water, then add 3 mL of sodium hydroxide solution to the hydroxycamptothecin solution, stir for 1 hour to fully dissolve the hydroxycamptothecin, add it to the gold nanoparticle solution prepared in step S2, stir for 24 hours to mix, and obtain a gold nano-camptothecin derivative solution;

[0010] S4: 1.8348 g of anhydrous zinc acetate was dissolved in 100 mL of methanol, 6.568 g of dimethylimidazole was dissolved in 300 mL of methanol, 300 mL of dimethylimidazole methanol solution was added to 100 mL of anhydrous zinc acetate methanol solution under vigorous stirring, and the mixture was reacted for 12 hours, and then centrifuged at 10000 r / min for ten minutes, washed with anhydrous methanol for three times, and finally vacuum dried at 60 degrees Celsius for 6 hours to obtain zeolite imidazole framework-8 nanoparticles;

[0011] S5: Take 50 mg of tannic acid and dissolve it in 2 mL of water, then add all the tannic acid solution to the zeolite imidazole framework-8 nanoparticles obtained in the above step S4, stir for ten minutes, and adjust the volume to 20 mL to prepare a hollow zeolite imidazole framework-8 solution;

[0012] S6: Add the gold nano-camptothecin derivative solution of step S3 to the hollow zeolite imidazole framework-8 solution prepared in step S5, stir for 12 hours, and stand for 12 hours to obtain a gold nano-zeolite imidazole framework-8 camptothecin derivative preparation.

[0013] As a preferred technical solution of the present invention, the molar ratio of 3-mercaptopropionic acid to PEG in step S2 is 1:33.

[0014] As a preferred technical solution of the present invention, the mass ratio of gold nanoparticles to camptothecin derivatives in step S3 is 1:1.5.

[0015] As a preferred technical solution of the present invention, the concentration ratio of anhydrous zinc acetate to dimethylimidazole in step S4 is 1:2.5-3.

[0016] As a preferred technical solution of the present invention, the mass ratio of the hollow zeolite imidazole framework-8 to the gold nano-camptothecin derivative solution in step S6 is 2:1.

[0017] As a preferred technical solution of the present invention, the particle size of the prepared zeolite imidazole framework-8 is 200-370nm, and the particle size of the gold nanoparticles is 12-15nm.

[0018] Compared with the prior art, the present invention has the following advantages:

[0019] Gold nanoparticles and hollow zeolite imidazole framework-8 can effectively carry hydroxycamptothecin. Gold nanoparticles act as photosensitizers and effectively convert near-infrared light into thermal energy, thereby enhancing the therapeutic effect of tumors. The zeolite imidazole framework-8 framework is acid-sensitive. This drug delivery system can achieve pH / NIR dual responsiveness to release drugs in the tumor microenvironment, and can be applied to the targeted treatment of anti-tumor drugs. It has good application prospects. Gold nanoparticles and zeolite imidazole framework-8 are used as carriers together to effectively improve the drug loading rate. In addition, gold nanoparticles themselves act as photosensitizers, effectively achieving the synergy of chemotherapy and photothermal therapy to improve the therapeutic effect. DETAILED DESCRIPTION

[0020] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only a part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0021] Embodiment 1:

[0022] The present invention provides a method for preparing a gold nano-zeolite imidazole framework-8 camptothecin derivative preparation, and the preparation method comprises the following steps:

[0023] S1: Dissolve 0.31 mL of chloroauric acid solution in 80 mL of distilled water, heat to boiling for ten minutes at 25°C in the dark, then add 0.20 g of sodium citrate to the solution, heat to boiling for 30 minutes, and let stand for 12 hours to obtain a gold nanoparticle solution with a particle size of 12 nm.

[0024] S2: Dissolve 0.0735g of 3-mercaptopropionic acid solution and 0.2262g of sodium hydroxide in 100mL of water respectively, adjust the pH of the gold nanoparticle solution prepared in the above step S1 to 11, then add 6mL of mercaptopropionic acid, stir for 2h, take the stirred prepared solution, add 50mmol of morpholineethanesulfonic acid to adjust the solution pH to 6.5, add 0.016g of EDC, add 0.02g of NHS, stir and react for 30 minutes, then add 0.0417g of PEG, stir at room temperature for 24 hours, the molar ratio of 3-mercaptopropionic acid to PEG is 1:33, and the prepared gold nanoparticle solution is obtained;

[0025] S3: Dissolve 0.0264 g of 10-hydroxycamptothecin in 10 mL of water, dissolve 1.6 g of sodium hydroxide in 10 mL of water, then add 3 mL of sodium hydroxide solution to the hydroxycamptothecin solution, stir for 1 hour to fully dissolve the hydroxycamptothecin, add it to the gold nanoparticle solution prepared in step S2, stir for 24 hours to mix, and obtain a gold nanoparticle-camptothecin derivative solution, wherein the mass ratio of gold nanoparticles to camptothecin derivatives is 1:1.5;

[0026] S4: 1.8348 g of anhydrous zinc acetate was dissolved in 100 mL of methanol, 6.568 g of dimethylimidazole was dissolved in 300 mL of methanol, 300 mL of dimethylimidazole methanol solution was added to 100 mL of anhydrous zinc acetate methanol solution under vigorous stirring, the concentration ratio of anhydrous zinc acetate to dimethylimidazole was 1:2.5, the mixture was reacted for 12 hours, and then centrifuged at 10000 r / min for ten minutes, washed with anhydrous methanol for 3 times, and finally vacuum dried at 60 degrees Celsius for 6 hours to obtain zeolite imidazole framework-8 nanoparticles, the particle size of zeolite imidazole framework-8 was 200 nm;

[0027] S5: Take 50 mg of tannic acid and dissolve it in 2 mL of water, then add all the tannic acid solution to the zeolite imidazole framework-8 nanoparticles obtained in the above step S4, stir for ten minutes, and adjust the volume to 20 mL to prepare a hollow zeolite imidazole framework-8 solution;

[0028] S6: Add the gold nano-camptothecin derivative solution of the above step S3 to the hollow zeolite imidazole framework-8 solution prepared in the above step S5, stir for 12 hours, and let stand for 12 hours to obtain a gold nano-zeolite imidazole framework-8 camptothecin derivative preparation. The mass ratio of the hollow zeolite imidazole framework-8 to the gold nano-camptothecin derivative solution is 2:1.

[0029] Embodiment 2:

[0030] The present invention provides a method for preparing a gold nano-zeolite imidazole framework-8 camptothecin derivative preparation, and the preparation method comprises the following steps:

[0031] S1: Dissolve 0.31 mL of chloroauric acid solution in 80 mL of distilled water, heat to boiling for ten minutes at 25°C in the dark, then add 0.20 g of sodium citrate to the solution, heat to boiling for 30 minutes, and let stand for 12 hours to obtain a gold nanoparticle solution with a particle size of 13 nm.

[0032] S2: Dissolve 0.0735g of 3-mercaptopropionic acid solution and 0.2262g of sodium hydroxide in 100mL of water respectively, adjust the pH of the gold nanoparticle solution prepared in the above step S1 to 11, then add 6mL of mercaptopropionic acid, stir for 2h, take the stirred prepared solution, add 50mmol of morpholineethanesulfonic acid to adjust the solution pH to 6.5, add 0.016g of EDC, add 0.02g of NHS, stir and react for 30 minutes, then add 0.0417g of PEG, stir at room temperature for 24 hours, the molar ratio of 3-mercaptopropionic acid to PEG is 1:33, and the prepared gold nanoparticle solution is obtained;

[0033] S3: Dissolve 0.0264 g of camptothecin in 10 mL of water, dissolve 1.6 g of sodium hydroxide in 10 mL of water, then add 3 mL of sodium hydroxide solution to the camptothecin solution, stir for 1 hour to fully dissolve hydroxycamptothecin, add it to the gold nanoparticle solution prepared in step S2, stir for 24 hours to mix, and obtain a gold nanoparticle-camptothecin derivative solution, wherein the mass ratio of gold nanoparticles to camptothecin derivatives is 1:1.5;

[0034] S4: 1.8348 g of anhydrous zinc acetate was dissolved in 100 mL of methanol, 6.568 g of dimethylimidazole was dissolved in 300 mL of methanol, 300 mL of dimethylimidazole methanol solution was added to 100 mL of anhydrous zinc acetate methanol solution under vigorous stirring, the concentration ratio of anhydrous zinc acetate to dimethylimidazole was 1:2.7, the reaction was continued for 12 hours, and then centrifuged at 10000 r / min for ten minutes, washed with anhydrous methanol for 3 times, and finally vacuum dried at 60 degrees Celsius for 6 hours to obtain zeolite imidazole framework-8 nanoparticles, the particle size of zeolite imidazole framework-8 was 250 nm;

[0035] S5: Take 50 mg of tannic acid and dissolve it in 2 mL of water, then add all the tannic acid solution to the zeolite imidazole framework-8 nanoparticles obtained in the above step S4, stir for ten minutes, and adjust the volume to 20 mL to prepare a hollow zeolite imidazole framework-8 solution;

[0036] S6: Add the gold nano-camptothecin derivative solution of the above step S3 to the hollow zeolite imidazole framework-8 solution prepared in the above step S5, stir for 12 hours, and let stand for 12 hours to obtain a gold nano-zeolite imidazole framework-8 camptothecin derivative preparation. The mass ratio of the hollow zeolite imidazole framework-8 to the gold nano-camptothecin derivative solution is 2:1.

[0037] Embodiment 3:

[0038] The present invention provides a method for preparing a gold nano-zeolite imidazole framework-8 camptothecin derivative preparation, and the preparation method comprises the following steps:

[0039] S1: Dissolve 0.31 mL of chloroauric acid solution in 80 mL of distilled water, heat to boiling for ten minutes at 25°C in the dark, then add 0.20 g of sodium citrate to the solution, heat to boiling for 30 minutes, and let stand for 12 hours to obtain a gold nanoparticle solution with a particle size of 14 nm.

[0040] S2: Dissolve 0.0735g of 3-mercaptopropionic acid solution and 0.2262g of sodium hydroxide in 100mL of water respectively, adjust the pH of the gold nanoparticle solution prepared in the above step S1 to 11, then add 6mL of mercaptopropionic acid, stir for 2h, take the stirred prepared solution, add 50mmol of morpholineethanesulfonic acid to adjust the solution pH to 6.5, add 0.016g of EDC, add 0.02g of NHS, stir and react for 30 minutes, then add 0.0417g of PEG, stir at room temperature for 24 hours, the molar ratio of 3-mercaptopropionic acid to PEG is 1:33, and the prepared gold nanoparticle solution is obtained;

[0041] S3: Dissolve 0.0264 g of irinotecan hydrochloride (CPT-11) in 10 mL of water, dissolve 1.6 g of sodium hydroxide in 10 mL of water, then add 3 mL of sodium hydroxide solution to the CPT-11 solution, stir for 1 hour to fully dissolve CPT-11, add it to the gold nanoparticle solution prepared in step S2, stir for 24 hours to mix, and obtain a gold nanoparticle-camptothecin derivative solution, wherein the mass ratio of gold nanoparticles to camptothecin derivatives is 1:1.5;

[0042] S4: 1.8348 g of anhydrous zinc acetate was dissolved in 100 mL of methanol, 6.568 g of dimethylimidazole was dissolved in 300 mL of methanol, 300 mL of dimethylimidazole methanol solution was added to 100 mL of anhydrous zinc acetate methanol solution under vigorous stirring, the concentration ratio of anhydrous zinc acetate to dimethylimidazole was 1:2.8, the mixture was reacted for 12 hours, and then centrifuged at 10000 r / min for ten minutes, washed with anhydrous methanol for 3 times, and finally vacuum dried at 60 degrees Celsius for 6 hours to obtain zeolite imidazole framework-8 nanoparticles, the particle size of zeolite imidazole framework-8 was 290 nm;

[0043] S5: Take 50 mg of tannic acid and dissolve it in 2 mL of water, then add all the tannic acid solution to the zeolite imidazole framework-8 nanoparticles obtained in the above step S4, stir for ten minutes, and adjust the volume to 20 mL to prepare a hollow zeolite imidazole framework-8 solution;

[0044] S6: Add the gold nano-camptothecin derivative solution of the above step S3 to the hollow zeolite imidazole framework-8 solution prepared in the above step S5, stir for 12 hours, and let stand for 12 hours to obtain a gold nano-zeolite imidazole framework-8 camptothecin derivative preparation. The mass ratio of the hollow zeolite imidazole framework-8 to the gold nano-camptothecin derivative solution is 2:1.

[0045] Embodiment 4:

[0046] The present invention provides a method for preparing a gold nano-zeolite imidazole framework-8 camptothecin derivative preparation, and the preparation method comprises the following steps:

[0047] S1: Dissolve 0.31 mL of chloroauric acid solution in 80 mL of distilled water, heat to boiling for ten minutes at 25°C in the dark, then add 0.20 g of sodium citrate to the solution, heat to boiling for 30 minutes, and let stand for 12 hours to obtain a gold nanoparticle solution with a particle size of 15 nm.

[0048] S2: Dissolve 0.0735g of 3-mercaptopropionic acid solution and 0.2262g of sodium hydroxide in 100mL of water respectively, adjust the pH of the gold nanoparticle solution prepared in the above step S1 to 11, then add 6mL of mercaptopropionic acid, stir for 2h, take the stirred prepared solution, add 50mmol of morpholineethanesulfonic acid to adjust the solution pH to 6.5, add 0.016g of EDC, add 0.02g of NHS, stir and react for 30 minutes, then add 0.0417g of PEG, stir at room temperature for 24 hours, the molar ratio of 3-mercaptopropionic acid to PEG is 1:33, and the prepared gold nanoparticle solution is obtained;

[0049] S3: Dissolve 0.0264 g of 10-hydroxycamptothecin in 10 mL of water, dissolve 1.6 g of sodium hydroxide in 10 mL of water, then add 3 mL of sodium hydroxide solution to the hydroxycamptothecin solution, stir for 1 hour to fully dissolve the hydroxycamptothecin, add it to the gold nanoparticle solution prepared in step S2, stir for 24 hours to mix, and obtain a gold nanoparticle-camptothecin derivative solution, wherein the mass ratio of gold nanoparticles to camptothecin derivatives is 1:1.5;

[0050] S4: 1.8348 g of anhydrous zinc acetate was dissolved in 100 mL of methanol, 6.568 g of dimethylimidazole was dissolved in 300 mL of methanol, 300 mL of dimethylimidazole methanol solution was added to 100 mL of anhydrous zinc acetate methanol solution under vigorous stirring, the concentration ratio of anhydrous zinc acetate to dimethylimidazole was 1:3, the reaction was continued for 12 hours, and then centrifuged at 10000 r / min for ten minutes, washed with anhydrous methanol for 3 times, and finally vacuum dried at 60 degrees Celsius for 6 hours to obtain zeolite imidazole framework-8 nanoparticles, the particle size of zeolite imidazole framework-8 was 350 nm;

[0051] S5: Take 50 mg of tannic acid and dissolve it in 2 mL of water, then add all the tannic acid solution to the zeolite imidazole framework-8 nanoparticles obtained in the above step S4, stir for ten minutes, and adjust the volume to 20 mL to prepare a hollow zeolite imidazole framework-8 solution;

[0052] S6: Add the gold nano-camptothecin derivative solution of the above step S3 to the hollow zeolite imidazole framework-8 solution prepared in the above step S5, stir for 12 hours, and let stand for 12 hours to obtain a gold nano-zeolite imidazole framework-8 camptothecin derivative preparation. The mass ratio of the hollow zeolite imidazole framework-8 to the gold nano-camptothecin derivative solution is 2:1.

[0053] The present invention discloses a method for preparing a gold nano-zeolite imidazole framework-8 camptothecin derivative preparation. Gold nanoparticles and hollow zeolite imidazole framework-8 inorganic frameworks can effectively carry hydroxycamptothecin. Gold nanoparticles are used as photosensitizers to effectively convert near-infrared light into heat energy, thereby enhancing the therapeutic effect of tumors. Since the zeolite imidazole framework-8 framework is acid-sensitive, the drug-carrying system can achieve pH / NIR dual-responsive drug release in the tumor microenvironment, can be applied to the targeted treatment of anti-tumor drugs, and has good application prospects.

[0054] Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned implementation cases, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for preparing a gold nano-zeolite imidazole framework-8 camptothecin derivative preparation, characterized in that The preparation method consists of the following steps: S1: Dissolve 0.31 mL of chloroauric acid solution in 80 mL of distilled water, heat to boiling for ten minutes at 25°C in the dark, then add 0.20 g of sodium citrate to the solution, heat to boiling for 30 minutes, and let stand for 12 hours to obtain a gold nanoparticle solution; S2: Dissolve 0.0735g of 3-mercaptopropionic acid solution and 0.2262g of sodium hydroxide in 100mL of water respectively, adjust the pH of the gold nanoparticle solution prepared in the above step S1 to 11, then add 6mL of mercaptopropionic acid, stir for 2h, take the solution prepared by stirring, add 50mmol of morpholineethanesulfonic acid to adjust the solution pH to 6.5, add 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (EDC) 0.016g, add N-hydroxysuccinimide (NHS) 0.02g, stir and react for 30 minutes, then add polyethylene glycol (PEG) 0.0417g, stir at room temperature for 24 hours to obtain the prepared gold nanoparticle solution; S3: Dissolve 0.0264 g of 10-hydroxycamptothecin in 10 mL of water, dissolve 1.6 g of sodium hydroxide in 10 mL of water, then add 3 mL of sodium hydroxide solution to the hydroxycamptothecin solution, stir for 1 hour to fully dissolve the hydroxycamptothecin, add it to the gold nanoparticle solution prepared in step S2, stir for 24 hours to mix, and obtain a gold nano-camptothecin derivative solution; S4: 1.8348 g of anhydrous zinc acetate was dissolved in 100 mL of methanol, 6.568 g of dimethylimidazole was dissolved in 300 mL of methanol, 300 mL of dimethylimidazole methanol solution was added to 100 mL of anhydrous zinc acetate methanol solution under vigorous stirring, and the mixture was reacted for 12 hours, and then centrifuged at 10000 r / min for ten minutes, washed with anhydrous methanol for three times, and finally vacuum dried at 60 degrees Celsius for 6 hours to obtain zeolite imidazole framework-8 nanoparticles; S5: Take 50 mg of tannic acid and dissolve it in 2 mL of water, then add all the tannic acid solution to the zeolite imidazole framework-8 nanoparticles obtained in the above step S4, stir for ten minutes, and adjust the volume to 20 mL to prepare a hollow zeolite imidazole framework-8 solution; S6: Add the gold nano-camptothecin derivative solution of step S3 to the hollow zeolite imidazole framework-8 solution prepared in step S5, stir for 12 hours, and stand for 12 hours to obtain a gold nano-zeolite imidazole framework-8 camptothecin derivative preparation.

2. The method for preparing a gold nano-zeolite imidazole framework-8 camptothecin derivative preparation according to claim 1, characterized in that: The molar ratio of 3-mercaptopropionic acid to PEG in step S2 is 1:

33.

3. The method for preparing a gold nano-zeolite imidazole framework-8 camptothecin derivative preparation according to claim 1, characterized in that: In the step S3, the mass ratio of the gold nanoparticles to the camptothecin derivatives is 1:1.

5.

4. The method for preparing a gold nano-zeolite imidazole framework-8 camptothecin derivative preparation according to claim 1, characterized in that: In step S4, the concentration ratio of anhydrous zinc acetate to dimethylimidazole is 1:2.5-3.

5. The method for preparing a gold nano-zeolite imidazole framework-8 camptothecin derivative preparation according to claim 1, characterized in that: In the step S6, the mass ratio of the hollow zeolite imidazole framework-8 to the gold nano-camptothecin derivative solution is 2:

1.

6. The method for preparing a gold nano-zeolite imidazole framework-8 camptothecin derivative preparation according to claim 1, characterized in that: The particle size of the prepared zeolite imidazole framework-8 is 200-370nm, and the particle size of the gold nanoparticle is 12-15nm.

7. The method for preparing a gold nano-zeolite imidazole framework-8 camptothecin derivative preparation according to claim 1, characterized in that: The drug loading rate was 23.12%±2%, and the encapsulation efficiency of the system was 60%±2%.