Organic fertilizer for planting tea leaves in original habitat and preparation method of organic fertilizer

Abstract

The invention belongs to the technical field of organic fertilizers, and particularly relates to an organic fertilizer for original habitat tea planting and a preparation method thereof, the organic fertilizer for original habitat tea planting comprises the following components by weight: 10-20 parts of a CS-EDTA compound, 5-10 parts of a tea waste residue extract, 30-40 parts of an animal waste fermentation product, 10-20 parts of bentonite, and 2.5-5 parts of purified water. According to the prepared organic fertilizer, natural degradable materials are adopted, necessary and specific nutrient substances for growth of original habitat tea can be provided, and the prepared CS-EDTA compound can serve as a carrier to control release of nutrients and reduce the fertilization frequency, has the functions of resisting bacteria, forming a film and chelating heavy metal ions, protects the root system of a tea tree and improves the survival rate of the tea tree. The soil ecological environment for tea growth in the original habitat is maintained.

Description

Technical Field

[0001] The invention belongs to the technical field of organic fertilizers, and in particular relates to an organic fertilizer for native tea planting and a preparation method thereof. Background Art

[0002] As a perennial plant with extremely high economic value, the growth environment and soil conditions of tea trees are crucial to the formation of tea quality. In particular, native tea cultivation relies on a specific natural environment and usually has better sensory quality and drinking value, so the requirements for fertilization and management are also more stringent. In the native environment, the roots of tea trees must maintain a high degree of coordination with soil microorganisms, relying on the help of microorganisms to absorb nutrients and improve their own resistance. However, due to its fragility, it is easy to be invaded by soil pathogens, coupled with the disturbance of conventional chemical fertilizers and improper fertilization methods, this natural ecological balance can be easily destroyed, affecting the growth of tea trees and the quality of tea. Therefore, in native tea cultivation, how to ensure a stable supply of nutrients while protecting the roots of tea trees from diseases has become an important challenge in the current tea planting management.

[0003] Slow-release fertilizers are widely used in modern agriculture, and are particularly suitable for the long-term nutrient supply of perennial tea trees. However, most of the existing slow-release fertilizers use chemical coating technology. Although the slow release of nutrients is achieved to a certain extent, the coating materials are mostly non-degradable or difficult-to-degrade chemical substances. Long-term use may lead to the accumulation of chemical residues in the soil, destroying the natural ecosystem of the native soil of the tea tree. In addition, slow-release fertilizers often have a single function and cannot achieve an effective combination of supplying nutrition, slow-release nutrients, and protecting the root system of crops. Therefore, there is an urgent need for a novel organic slow-release fertilizer that can help tea trees resist pathogens, has slow-release ability, and can effectively protect the root system of tea trees, maintain the ecological balance of the native tea garden soil, and the healthy growth of tea trees. Summary of the invention

[0004] In view of the above situation, the present invention provides an organic fertilizer for native tea planting and a preparation method thereof. Chitosan (CS) is used as an organic fertilizer carrier. After ethylenediaminetetraacetic acid (EDTA) is introduced into its structure, the formed CS-EDTA complex can effectively encapsulate the nutrients, active bacteria and soil conditioners in the fertilizer. It also has excellent film-forming properties and chelating functions, protecting the roots of tea trees from the invasion of pathogens, and adsorbing heavy metals and harmful substances in the soil through its chelating properties to purify the soil environment. Animal waste and tea waste residue extracts are the main nutrient sources of organic fertilizers. They are rich in basic nutrients such as nitrogen, phosphorus, and potassium, as well as specific nutrients required for the growth of native tea trees, promoting the high-quality growth of tea leaves, and effectively solving the limitations of existing fertilizer technology in native planting.

[0005] In order to achieve the above object, the technical solution adopted by the present invention is as follows: The invention provides an organic fertilizer for native tea planting. The organic fertilizer comprises the following components in parts by weight: 10-20 parts of CS-EDTA complex (chitosan-ethylenediaminetetraacetic acid complex), 5-10 parts of tea waste extract, 30-40 parts of animal waste fermentation products, 10-20 parts of bentonite, and 2.5-5 parts of purified water.

[0006] Furthermore, the molecular weight of CS in the CS-EDTA complex is 50-400 kDa, and the degree of deacetylation is 85%-95%.

[0007] Preferably, in order to facilitate dissolution preparation, the EDTA in the CS-EDTA complex is selected in the form of its disodium salt, namely EDTA-2Na.

[0008] The CS-EDTA complex is prepared from the following raw materials: CS, EDTA-2Na, 2% acetic acid solution and deionized water. The preparation method of the CS-EDTA complex comprises the following steps: Step A1: weigh CS, EDTA-2Na, 2% acetic acid solution and deionized water in a mass ratio of 2:1:100:10; Step A2: Slowly add the CS weighed in step A1 into 2% acetic acid solution, place under a magnetic stirrer, stir at a speed of 200 r / min, and at a temperature of 35°C, and continue stirring for 1 h to completely dissolve it, to obtain a CS solution; Step A3: dissolve the EDTA-2Na weighed in step A1 in deionized water, and stir manually for 3 min until the EDTA-2Na is completely dissolved to obtain an EDTA-2Na solution; Step A4: slowly add the EDTA-2Na solution prepared in step A3 to the CS solution prepared in step A2, with continuous magnetic stirring during the process. After complete addition, continue stirring for 2 h at a stirring speed of 120 r / min and a stirring temperature of 35°C to allow sufficient reaction to obtain a CS-EDTA complex solution. Step A5: The CS-EDTA complex solution obtained in step A4 is naturally cooled to obtain a CS-EDTA complex.

[0009] The tea waste residue extract is prepared from tea waste residue, which includes tea leaf residue, tea stems and tea powder discarded in the process of tea production and processing. The preparation method of the tea waste residue extract comprises the following steps: Step B1: Weigh tea waste residue and deionized water in a mass ratio of 1:20, wash and dry the tea waste residue, grind it with a grinder, and soak it in deionized water for 30 minutes. After soaking, place it in a constant temperature water bath for extraction at 80°C for 2 hours to obtain an extract mixture; Step B2: filtering and separating the extract mixture in step B1 using a filter bag, and retaining the filtrate; Step B3: Concentrate the filtrate obtained in step B2 using a rotary evaporator at a temperature of 40°C and a speed of 40r / min. The rotary evaporator is connected to a low-temperature coolant circulation pump. The temperature of the low-temperature coolant circulation pump is -8°C. After the concentration is completed, an extract concentrate is obtained; Step B4: The extract concentrate obtained in step B3 is subjected to hot air drying at a temperature of 60° C. for 12 h to obtain a tea waste residue extract.

[0010] The animal waste fermentation product is prepared from the following raw materials: animal waste, straw and microbial powder. The animal waste fermentation product preparation process includes the following steps: Step C1: weighing animal waste, straw and microbial powder in a mass ratio of 4:1:0.02, manually removing debris and then mixing the animal waste and straw to obtain an animal waste-straw mixture; Step C2: adding microbial powder to the animal waste straw mixture obtained in step C1 for constant temperature fermentation at a temperature of 55° C. for 7 days to obtain fermented animal waste; Step C3: drying the fermented animal waste obtained in step C2 by hot air to remove moisture, the drying temperature is 70° C., and the drying time is 8 h, to obtain fermented dried animal waste; Step C4: Using a pulverizer, the fermented and dried animal waste obtained in step C3 is pulverized into particles or powders with a size of 2-4 mm to obtain animal waste fermentation products.

[0011] Furthermore, the animal waste is selected from one or more of cow dung, minced meat discarded in beef processing, fur, viscera, fat, and blood clots.

[0012] Preferably, the straw is corn straw.

[0013] Furthermore, the microbial powder is composed of the following raw materials in percentage by weight: 25% Streptomyces, 25% Bacillus subtilis, 10% Bacillus licheniformis, 10% Bacillus megaterium, 10% Pseudomonas fluorescens, 15% yeast, and 5% Lactobacillus paracasei.

[0014] The present invention also provides a method for preparing an organic fertilizer for native tea planting, which specifically comprises the following steps: (1): Weigh 10-20 parts of CS-EDTA complex, 5-10 parts of tea waste extract, 30-40 parts of animal waste fermentation product, 10-20 parts of bentonite, and 2.5-5 parts of purified water; (2): The bentonite weighed in step (1) and purified water were mixed in a mass ratio of 4:1, stirred for 2 min, and stirred at a speed of 30 r / min to obtain wet bentonite; (3): using a three-dimensional mixer, the weighed tea waste extract and animal waste fermentation product in step (1) are fully mixed, stirred for 3 min at a stirring speed of 50 r / min, and then the wet bentonite obtained in step (2) is added, the speed is increased to 100 r / min, and stirring is continued for 5 min to obtain an organic fertilizer intermediate product; (4): Add CS-EDTA complex to the organic fertilizer intermediate product obtained in step (3) and mix evenly at a stirring speed of 80 r / min for 7 min to obtain wet organic fertilizer; (5): using a granulator to make the wet organic fertilizer obtained in step (4) into organic fertilizer granules with a diameter of 5±1 mm; (6): Dry the organic fertilizer particles obtained in step (5) using a drying device at a drying temperature of 70° C. for 6 h to obtain an organic fertilizer for native tea cultivation.

[0015] The beneficial effects achieved by the present invention are as follows: The organic fertilizer for native tea planting prepared by the present invention is made of natural degradable materials and is designed according to the native tea tree growth environment and soil characteristics. The CS-EDTA complex has good adhesion and slow release, which can effectively coat the various components of the fertilizer, so that the nutrients can be gradually released over a long period of time, reduce the frequency of fertilization, and improve the fertilizer utilization rate. At the same time, the CS used in the present invention is in a macromolecular form, which is degraded into low molecular weight chitosan oligosaccharides under the action of soil microorganisms. Chitosan oligosaccharides have good antibacterial and film-forming properties, and can protect the tea tree roots from pathogens without affecting the absorption of nutrients by the tea tree, and reduce the impact of environmental stress. When the organic fertilizer is stored, the CS-EDTA complex can effectively chelate the beneficial metal ions (such as calcium, magnesium, iron, copper, etc.) in the fertilizer to prevent nutrient loss. After application, the chelated metal ions are continuously and slowly released under the degradation of soil microorganisms, thereby improving the effectiveness of the fertilizer. In addition, the modified EDTA can form a biofilm with oligosaccharides and stay in the tea tree root system for a long time, and further protect the tea tree root system by chelating heavy metal ions in the soil. As a natural source of organic matter, tea waste extract contains polyphenol compounds, amino acids, cellulose and trace elements, which are specific nutrients required by tea trees. It can not only improve nutrient utilization, but also improve soil structure, enhance soil water retention and air permeability, and complement animal waste fermentation to further improve the fertilizer efficiency. As the main source of organic matter, animal waste fermentation is rich in nutrients required for plant growth, such as nitrogen, phosphorus, and potassium. After sufficient fermentation, it not only removes harmful pathogens, but also improves the fertilizer efficiency of organic fertilizers and soil microbial activity. Bentonite, as a soil conditioner, works together with CS-EDTA complex to regulate the soil's water retention capacity and prevent nutrient loss. It also has the function of regulating the slow release of fertilizers and prolonging the action time of fertilizers.

[0016] The organic fertilizer for native tea planting prepared by the present invention uses raw materials with green, safe and non-toxic properties, is suitable for the growth of native tea trees, and protects the health of tea trees and maintains the soil environment while continuously providing nutrients. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 The present invention is a flow chart for preparing organic fertilizer for native tea cultivation; Figure 2 This is a graph showing the results of detecting soil organic matter content after applying the organic fertilizer prepared by the present invention for native tea cultivation.

[0018] The accompanying drawings are used to provide further understanding of the present invention and constitute a part of the specification. They are used to explain the present invention together with the embodiments of the present invention and do not constitute a limitation of the present invention. DETAILED DESCRIPTION

[0019] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments; based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0020] Unless otherwise defined, all professional and scientific terms used herein have the same meanings as those familiar to those skilled in the art. In addition, any methods and materials similar or equivalent to those described herein may be applied to the present invention. The preferred implementation methods and materials described herein are for demonstration purposes only and are not intended to limit the content of this application.

[0021] The preparation process in the following examples is referred to Figure 1 Unless otherwise specified, all methods are conventional; the materials used in the following embodiments, unless otherwise specified, are new materials purchased from the market.

[0022] Example 1: This example provides an organic fertilizer for native tea cultivation, the organic fertilizer comprising the following components in parts by weight: 15 parts of CS-EDTA complex, 5 parts of tea waste extract, 40 parts of animal waste fermentation, 10 parts of bentonite, and 2.5 parts of purified water; Furthermore, the CS-EDTA complex preparation method comprises the following steps: Step A1: Weigh 40 parts by weight of CS, 20 parts by weight of EDTA-2Na, 2000 parts by weight of 2% acetic acid solution and 200 parts by weight of deionized water; Step A2: Slowly add the CS weighed in step A1 into 2% acetic acid solution, place under a magnetic stirrer, stir at a speed of 200 r / min, and at a temperature of 35°C, and continue stirring for 1 h to completely dissolve it, to obtain a CS solution; Step A3: dissolve the EDTA-2Na weighed in step A1 in deionized water, and stir manually for 3 min until the EDTA-2Na is completely dissolved to obtain an EDTA-2Na solution; Step A4: slowly add the EDTA-2Na solution prepared in step A3 to the CS solution prepared in step A2, with continuous magnetic stirring during the process. After complete addition, continue stirring for 2 h at a stirring speed of 120 r / min and a stirring temperature of 35°C to allow sufficient reaction to obtain a CS-EDTA complex solution. Step A5: The CS-EDTA complex solution obtained in step A4 is naturally cooled to obtain a CS-EDTA complex.

[0023] Furthermore, the method for preparing the tea waste residue extract comprises the following steps: Step B1: Weigh 80 parts by weight of tea waste residue and 1600 parts by weight of deionized water, wash and dry the tea waste residue, grind it with a grinder, and soak it in deionized water for 30 minutes. After soaking, place it in a constant temperature water bath for extraction at 80°C for 2 hours to obtain an extract mixture; Step B2: filtering and separating the extract mixture in step B1 using a filter bag, and retaining the filtrate; Step B3: Concentrate the filtrate obtained in step B2 using a rotary evaporator at a temperature of 40°C and a speed of 40r / min. The rotary evaporator is connected to a low-temperature coolant circulation pump. The temperature of the low-temperature coolant circulation pump is -8°C. After the concentration is completed, an extract concentrate is obtained; Step B4: The extract concentrate obtained in step B3 is subjected to hot air drying at a temperature of 60° C. for 12 h to obtain a tea waste residue extract.

[0024] Furthermore, the animal waste fermentation product preparation process comprises the following steps: Step C1: weighing 100 parts by weight of cow dung, 25 parts by weight of corn stalks and 0.5 parts by weight of microbial powder, manually removing debris and then mixing the cow dung and corn stalks to obtain an animal waste straw mixture; Step C2: adding microbial powder to the animal waste straw mixture obtained in step C1 for constant temperature fermentation at a temperature of 55° C. for 7 days to obtain fermented animal waste; Step C3: drying the fermented animal waste obtained in step C2 by hot air to remove moisture, the drying temperature is 70° C., and the drying time is 8 h, to obtain fermented dried animal waste; Step C4: Using a pulverizer, the fermented and dried animal waste obtained in step C3 is pulverized into particles or powders with a size of 2-4 mm to obtain animal waste fermentation products.

[0025] This embodiment also provides a method for preparing organic fertilizer for native tea planting, which specifically comprises the following steps: (1): Weigh 15 parts of CS-EDTA complex, 5 parts of tea waste extract, 40 parts of animal waste fermentation product, 10 parts of bentonite, and 2.5 parts of purified water; (2): The bentonite weighed in step (1) and purified water were mixed, stirred for 2 min at a stirring speed of 30 r / min to obtain wet bentonite; (3): using a three-dimensional mixer, the tea waste extract and the animal waste fermentation product weighed in step (1) are fully mixed, stirred for 3 minutes at a stirring speed of 50 r / min, and then the wet bentonite obtained in step (2) is added, the speed is increased to 100 r / min, and stirring is continued for 5 minutes to obtain an organic fertilizer intermediate product; (4): Add CS-EDTA complex to the organic fertilizer intermediate product obtained in step (3) and mix evenly at a stirring speed of 80 r / min for 7 min to obtain wet organic fertilizer; (5): using a granulator to make the wet organic fertilizer obtained in step (4) into organic fertilizer granules with a diameter of 5±1 mm; (6): Dry the organic fertilizer particles obtained in step (5) using a drying device at a drying temperature of 70° C. for 6 h to obtain an organic fertilizer for native tea cultivation.

[0026] Example 2: This example provides an organic fertilizer for native tea cultivation, the organic fertilizer comprising the following components in parts by weight: 10 parts of CS-EDTA complex, 8 parts of tea waste extract, 30 parts of animal waste fermentation, 16 parts of bentonite, and 4 parts of purified water; Furthermore, the CS-EDTA complex preparation method comprises the following steps: Step A1: Weigh 40 parts by weight of CS, 20 parts by weight of EDTA-2Na, 2000 parts by weight of 2% acetic acid solution and 200 parts by weight of deionized water; Step A2: Slowly add the CS weighed in step A1 into 2% acetic acid solution, place under a magnetic stirrer, stir at a speed of 200 r / min, and at a temperature of 35°C, and continue stirring for 1 h to completely dissolve it, to obtain a CS solution; Step A3: dissolve the EDTA-2Na weighed in step A1 in deionized water, and stir manually for 3 min until the EDTA-2Na is completely dissolved to obtain an EDTA-2Na solution; Step A4: slowly add the EDTA-2Na solution prepared in step A3 to the CS solution prepared in step A2, with continuous magnetic stirring during the process. After complete addition, continue stirring for 2 h at a stirring speed of 120 r / min and a stirring temperature of 35°C to allow sufficient reaction to obtain a CS-EDTA complex solution. Step A5: The CS-EDTA complex solution obtained in step A4 is naturally cooled to obtain a CS-EDTA complex.

[0027] Furthermore, the method for preparing the tea waste residue extract comprises the following steps: Step B1: Weigh 80 parts by weight of tea waste residue and 1600 parts by weight of deionized water, wash and dry the tea waste residue, grind it with a grinder, and soak it in deionized water for 30 minutes. After soaking, place it in a constant temperature water bath for extraction at 80°C for 2 hours to obtain an extract mixture; Step B2: filtering and separating the extract mixture in step B1 using a filter bag, and retaining the filtrate; Step B3: Concentrate the filtrate obtained in step B2 using a rotary evaporator at a temperature of 40°C and a speed of 40r / min. The rotary evaporator is connected to a low-temperature coolant circulation pump. The temperature of the low-temperature coolant circulation pump is -8°C. After the concentration is completed, an extract concentrate is obtained; Step B4: The extract concentrate obtained in step B3 is subjected to hot air drying at a temperature of 60° C. for 12 h to obtain a tea waste residue extract.

[0028] Furthermore, the animal waste fermentation product preparation process comprises the following steps: Step C1: weighing 100 parts by weight of a mixture of ground meat, fur and blood clots discarded in beef processing, 25 parts by weight of corn stalks and 0.5 parts by weight of microbial powder, manually removing debris and then mixing the mixture of ground meat, fur and blood clots discarded in beef processing with the corn stalks to obtain an animal waste straw mixture; Step C2: adding microbial powder to the animal waste straw mixture obtained in step C1 for constant temperature fermentation at a temperature of 55° C. for 7 days to obtain fermented animal waste; Step C3: drying the fermented animal waste obtained in step C2 by hot air to remove moisture, the drying temperature is 70° C., and the drying time is 8 h, to obtain fermented dried animal waste; Step C4: Using a pulverizer, the fermented and dried animal waste obtained in step C3 is pulverized into particles or powders with a size of 2-4 mm to obtain animal waste fermentation products.

[0029] This embodiment also provides a method for preparing organic fertilizer for native tea planting, which specifically comprises the following steps: (1): Weigh 10 parts of CS-EDTA complex, 8 parts of tea waste extract, 30 parts of animal waste fermentation product, 16 parts of bentonite, and 4 parts of purified water; (2): The bentonite weighed in step (1) and purified water were mixed, stirred for 2 min at a stirring speed of 30 r / min to obtain wet bentonite; (3): using a three-dimensional mixer, the tea waste extract and the animal waste fermentation product weighed in step (1) are fully mixed, stirred for 3 minutes at a stirring speed of 50 r / min, and then the wet bentonite obtained in step (2) is added, the speed is increased to 100 r / min, and stirring is continued for 5 minutes to obtain an organic fertilizer intermediate product; (4): Add CS-EDTA complex to the organic fertilizer intermediate product obtained in step (3) and mix evenly at a stirring speed of 80 r / min for 7 min to obtain wet organic fertilizer; (5): using a granulator to make the wet organic fertilizer obtained in step (4) into organic fertilizer granules with a diameter of 5±1 mm; (6): Dry the organic fertilizer particles obtained in step (5) using a drying device at a drying temperature of 70° C. for 6 h to obtain an organic fertilizer for native tea cultivation.

[0030] Example 3: This example provides an organic fertilizer for native tea cultivation, the organic fertilizer comprising the following components in parts by weight: 20 parts of CS-EDTA complex, 10 parts of tea waste extract, 35 parts of animal waste fermentation, 20 parts of bentonite, and 5 parts of purified water; Furthermore, the CS-EDTA complex preparation method comprises the following steps: Step A1: Weigh 40 parts by weight of CS, 20 parts by weight of EDTA-2Na, 2000 parts by weight of 2% acetic acid solution and 200 parts by weight of deionized water; Step A2: Slowly add the CS weighed in step A1 into 2% acetic acid solution, place under a magnetic stirrer, stir at a speed of 200 r / min, and at a temperature of 35°C, and continue stirring for 1 h to completely dissolve it, to obtain a CS solution; Step A3: dissolve the EDTA-2Na weighed in step A1 in deionized water, and stir manually for 3 min until the EDTA-2Na is completely dissolved to obtain an EDTA-2Na solution; Step A4: slowly add the EDTA-2Na solution prepared in step A3 to the CS solution prepared in step A2, with continuous magnetic stirring during the process. After complete addition, continue stirring for 2 h at a stirring speed of 120 r / min and a stirring temperature of 35°C to allow sufficient reaction to obtain a CS-EDTA complex solution. Step A5: The CS-EDTA complex solution obtained in step A4 is naturally cooled to obtain a CS-EDTA complex.

[0031] Furthermore, the method for preparing the tea waste residue extract comprises the following steps: Step B1: Weigh 80 parts by weight of tea waste residue and 1600 parts by weight of deionized water, wash and dry the tea waste residue, grind it with a grinder, and soak it in deionized water for 30 minutes. After soaking, place it in a constant temperature water bath for extraction at 80°C for 2 hours to obtain an extract mixture; Step B2: filtering and separating the extract mixture in step B1 using a filter bag, and retaining the filtrate; Step B3: Concentrate the filtrate obtained in step B2 using a rotary evaporator at a temperature of 40°C and a speed of 40r / min. The rotary evaporator is connected to a low-temperature coolant circulation pump. The temperature of the low-temperature coolant circulation pump is -8°C. After the concentration is completed, an extract concentrate is obtained; Step B4: The extract concentrate obtained in step B3 is subjected to hot air drying at a temperature of 60° C. for 12 h to obtain a tea waste residue extract.

[0032] Furthermore, the animal waste fermentation product preparation process comprises the following steps: Step C1: weighing 100 parts by weight of cow dung, 25 parts by weight of corn stalks and 0.5 parts by weight of microbial powder, manually removing debris and then mixing the cow dung and corn stalks to obtain an animal waste straw mixture; Step C2: adding microbial powder to the animal waste straw mixture obtained in step C1 for constant temperature fermentation at a temperature of 55° C. for 7 days to obtain fermented animal waste; Step C3: drying the fermented animal waste obtained in step C2 by hot air to remove moisture, the drying temperature is 70° C., and the drying time is 8 h, to obtain fermented dried animal waste; Step C4: Using a pulverizer, the fermented and dried animal waste obtained in step C3 is pulverized into particles or powders with a size of 2-4 mm to obtain animal waste fermentation products.

[0033] This embodiment also provides a method for preparing organic fertilizer for native tea planting, which specifically comprises the following steps: (1): Weigh 20 parts of CS-EDTA complex, 10 parts of tea waste extract, 35 parts of animal waste fermentation product, 20 parts of bentonite, and 5 parts of purified water; (2): The bentonite weighed in step (1) and purified water were mixed, stirred for 2 min at a stirring speed of 30 r / min to obtain wet bentonite; (3): using a three-dimensional mixer, the tea waste extract and the animal waste fermentation product weighed in step (1) are fully mixed, stirred for 3 minutes at a stirring speed of 50 r / min, and then the wet bentonite obtained in step (2) is added, the speed is increased to 100 r / min, and stirring is continued for 5 minutes to obtain an organic fertilizer intermediate product; (4): Add CS-EDTA complex to the organic fertilizer intermediate product obtained in step (3) and mix evenly at a stirring speed of 80 r / min for 7 min to obtain wet organic fertilizer; (5): using a granulator to make the wet organic fertilizer obtained in step (4) into organic fertilizer granules with a diameter of 5±1 mm; (6): Dry the organic fertilizer particles obtained in step (5) using a drying device at a drying temperature of 70° C. for 6 h to obtain an organic fertilizer for native tea cultivation.

[0034] The difference between Comparative Example 1 and Example 1 is that no CS-EDTA complex is added, and the rest is the same as Example 1; The difference between Comparative Example 2 and Example 1 is that no tea waste residue extract is added, and the rest is the same as Example 1; The difference between Comparative Example 3 and Example 1 is that no animal waste fermentation product is added, and the rest is the same as Example 1; The difference between Comparative Example 4 and Example 1 is that bentonite and purified water are not added, and the rest is the same as Example 1; Obviously, the above comparative examples and embodiments are only a part of comparative examples and embodiments of the present invention, and they and the comparative examples and embodiments based on such references are all within the scope of protection of the present invention.

[0035] In order to further illustrate the beneficial technical effects of the embodiments of the present invention, the effects of the organic fertilizers of Examples 1-3 and Comparative Examples 1-4 of the present invention were tested respectively. The testing method is as follows: the organic fertilizers prepared in Examples 1-3 and Comparative Examples 1-4 were applied to the native tea trees in the same growth environment in different test areas, and no fertilizer was applied to the blank group. The area of ​​each test area was 25 m 2 The planting density of tea trees is the same, the amount of fertilizer applied is 1.5 kg, and a one-time hole application is adopted. After the tea trees sprout, the growth conditions of the tea trees are observed and the soil quality is tested.

[0036] Determination of tea tree growth index In each test area, (0.33×0.33) m 2 (0.1 m 2) area, 5 places were taken in each test area, the number of tea tree sprouts in the extracted area was recorded, the sprout density was calculated, and the average value of 5 times was taken as the experimental result; the determination of 100 bud weight was when the tea tree shoots grew to one bud and two or three leaves, one bud and two leaves of the tea tree were picked to determine the 100 bud weight, 100 tea tree shoots with the same number of leaves were randomly picked in each test area, mixed, weighed and recorded, and the sampling was repeated 5 times, and the average value was taken for calculation. The test results of Examples 1-3, Comparative Examples 1-4 and the blank group for the effects of organic fertilizers used for native tea planting on the sprout density and 100 bud weight of tea trees are shown in Table 1.

[0037] Fresh leaf yield determination method The weight of the buds was weighed separately after each picking in each test area, and the total weight of each batch was added up to obtain the total yield of each test area. The results are shown in Table 1.

[0038] Table 1 Germination density, 100 bud weight and tea yield of tea trees under different fertilization treatments

[0039] It can be seen from the results in Table 1 that the organic fertilizer provided by this scheme for native tea cultivation has a better effect. Compared with the blank group and comparative examples 1-4, the germination density of tea trees is increased, the yield is increased, and it has a higher economic value.

[0040] To further verify the effect of the organic fertilizer prepared by the present invention on the native tea planting soil, the pH and organic matter content of the soil in the test area of ​​Examples 1-3, Comparative Examples 1-4 and the blank group were measured before fertilization, after fertilization, during the germination period and after picking.

[0041] Soil pH Determination Method The soil of each test area was sampled according to the "five-point method" before and after fertilization, during the germination period and after picking. 20 g of air-dried samples sieved with a 2 mm aperture were placed in a 50 mL beaker, and 40 mL of degassed water (removing CO2) was added. The mixture was stirred with a blender for 1 min to completely disperse the soil particles. After 30 min, the pH meter calibrated with the standard solution was used for measurement. The electrode was placed in the solution to be tested. When the displayed data was stable, the reading was taken and the pH value of the solution was recorded. Before measuring the next sample, the electrode was cleaned with distilled water and wiped dry with filter paper before use. The average soil pH value of 5 locations in each test area was taken, and the results are shown in Table 2.

[0042] Determination of soil organic matter content The heating method was used to determine the organic matter content in the soil. The soil of each test area was sampled according to the "five-point method" before and after fertilization, during the germination period and after picking. After the soil sample was air-dried and ground through a 0.5 mm sieve, 1.0 g was weighed and transferred to a 500 mL conical flask. 10.00 mL of 1.0 mol / L potassium dichromate standard solution was added and gently shaken. Then 20 mL of concentrated sulfuric acid was quickly added and shaken for 1 min to fully mix the soil and reagent. The conical flask was placed on an asbestos board for 30 min, 200 mL of deionized water and 3 drops of o-phenanthroline indicator were added, and 0.5 mol / L ammonium ferrous sulfate standard solution was used to titrate until brown-red, which was the titration end point. The volume of ammonium ferrous sulfate standard solution consumed was recorded. In the blank test, the soil sample was replaced with quartz sand, and the other operating steps were the same as those for soil sample analysis. The soil organic matter content was calculated according to the following formula, and the results of each test area were averaged. The results are shown in Figure 2 .

[0043] Soil organic matter content (g / kg) = C (V0-V) × 0.003 × 1.724 × 1000 / m In the formula: C is the concentration of ammonium ferrous sulfate standard solution, mol / L; V0 is the volume of ammonium ferrous sulfate standard solution consumed in the blank test, mL; V is the volume of ammonium ferrous sulfate standard solution consumed in the soil sample test, mL; m is the mass of the air-dried soil sample, g.

[0044] Table 2 Soil pH of tea plantations in situ under different fertilization treatments

[0045] As shown in Table 2, the organic fertilizer for native tea planting provided by the present invention has little effect on the soil pH of native tea growth before fertilization, after fertilization, during the germination period and after picking, while the soil pH changes greatly after fertilization in Comparative Example 1. It can be seen that the CS-EDTA complex, as a slow-release material, can effectively control the release rate of nutrients in the organic fertilizer and reduce the impact of sudden changes in the external environment on the tea tree. The tea tree is an acid-loving plant with an optimal pH range of 4.5-5.5. The organic fertilizer prepared in this scheme can alleviate the problem of soil alkalinization.

[0046] Figure 2 The organic matter content in the soil is measured at different stages. From the results, it can be seen that the organic fertilizer for native tea planting provided by the present invention can stably and continuously supply nutrients to tea trees at different stages, has a good slow-release effect, can reduce the frequency of fertilization, and ensure the native environment for the growth of tea trees.

[0047] In summary, the experimental results of the embodiments and comparative examples show that the present invention prepares an organic fertilizer that can be used for native tea tree planting through natural degradable materials, and the synthesized CS-EDTA complex will play a role in slow-release nutrients. While reducing the number of fertilizations, it can continuously and stably supply nutrients to tea trees and maintain the native soil environment, and the chitosan oligosaccharides formed after degradation not only have certain antibacterial properties, but also can form biofilms to protect soil structure. Tea waste residue extracts and animal waste fermentation products can efficiently provide essential nutrients for native tea trees, which is conducive to the healthy growth of tea trees.

[0048] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

[0049] The present invention and its implementation methods are described above, which is not restrictive. The drawings are only one of the implementation methods of the present invention, and the actual application is not limited thereto. In short, if ordinary technicians in the field are inspired by it and design methods and embodiments similar to the technical solution without creativity without departing from the purpose of the invention, they should all fall within the protection scope of the present invention.

Claims

1. An organic fertilizer for native tea planting, characterized in that: The method is prepared from the following raw materials in parts by weight: 10-20 parts of CS-EDTA complex, 5-10 parts of tea waste extract, 30-40 parts of animal waste fermentation product, 10-20 parts of bentonite, and 2.5-5 parts of purified water; The raw materials for preparing the CS-EDTA complex include CS, EDTA-2Na, 2% acetic acid solution and deionized water; The tea waste residue extract is prepared from tea waste residue; The raw materials for preparing the animal waste fermentation product include animal waste, straw and microbial powder.

2. The organic fertilizer for tea planting in situ according to claim 1, characterized in that: The preparation method of the CS-EDTA complex comprises the following steps: Step A1: weigh CS, EDTA-2Na, 2% acetic acid solution and deionized water; Step A2: slowly add the CS weighed in step A1 into 2% acetic acid solution and stir magnetically to completely dissolve it to obtain a CS solution; Step A3: Add the EDTA-2Na weighed in step A1 into deionized water and stir until completely dissolved to obtain an EDTA-2Na solution; Step A4: slowly add the EDTA-2Na solution prepared in step A3 to the CS solution prepared in step A2, and continue magnetic stirring to allow the reaction to complete, thereby obtaining a CS-EDTA complex solution; Step A5: The CS-EDTA complex solution obtained in step A4 is naturally cooled to obtain a CS-EDTA complex.

3. The organic fertilizer for native tea planting according to claim 2, characterized in that: The mass ratio of the CS, EDTA-2Na, 2% acetic acid solution and deionized water is 2:1:100:

10. The magnetic stirring is carried out in a magnetic stirrer. In step A2, the stirring speed is 200 r / min, the stirring temperature is 35°C, and the stirring time is 1 h. In step A3, the stirring time is 3 min. In step A4, the stirring speed is 120 r / min. After the EDTA-2Na solution is completely added to the CS solution, the stirring time is continued for 2 h.

4. The organic fertilizer for native tea planting according to claim 1, characterized in that: The preparation method of the tea waste residue extract comprises the following steps: Step B1: weighing tea waste residue and deionized water, washing, drying, and crushing the tea waste residue, and then soaking it in deionized water, and then heating and extracting it to obtain an extract mixture; Step B2: filtering and separating the extract mixture obtained in step B1, and retaining the filtrate; Step B3: Concentrating the filtrate obtained in step B2 to obtain an extract concentrate; Step B4: Drying the extract concentrate obtained in step B3 to obtain a tea waste extract.

5. The organic fertilizer for in situ tea planting according to claim 4, characterized in that: In step B1, the mass ratio of tea waste residue to deionized water is 1:20, the soaking time is 30 min, the heating process is carried out in a constant temperature water bath, the extraction temperature is 80°C, and the extraction time is 2 h. In step B2, a filter bag is used in the filtration process. In step B3, the concentration process is carried out in a rotary evaporator at a temperature of 40°C and a speed of 40 r / min. The rotary evaporator is connected to a low-temperature coolant circulation pump, and the temperature of the low-temperature coolant circulation pump is -8°C. In step B4, the drying method is hot air drying at a temperature of 60°C and a drying time of 12 h.

6. The organic fertilizer for native tea planting according to claim 1, characterized in that: The method for preparing the animal waste fermentation product comprises the following steps: Step C1: weighing animal waste, straw and microbial powder, removing debris and mixing the animal waste and straw to obtain an animal waste-straw mixture; Step C2: adding microbial powder to the animal waste straw mixture obtained in step C1 for fermentation to obtain fermented animal waste; Step C3: drying to remove moisture from the fermented animal waste obtained in step C2 to obtain fermented dried animal waste; Step C4: crushing the fermented and dried animal waste obtained in step C3 to obtain animal waste fermentation product.

7. The organic fertilizer for in situ tea planting according to claim 6, characterized in that: The microbial powder is composed of the following raw materials in weight percentage: 25% of Streptomyces, 25% of Bacillus subtilis, 10% of Bacillus licheniformis, 10% of Bacillus megaterium, 10% of Pseudomonas fluorescens, 15% of yeast, and 5% of Lactobacillus paracasei. The mass ratio of the animal waste, straw and microbial powder is 4:1:0.

02. The fermentation temperature in step C2 is 55°C and the fermentation time is 7 days. The drying method in step C3 is hot air drying, the drying temperature is 70°C, and the drying time is 8 hours. The crushing process in step C4 is carried out in a crusher, and the particle size after crushing is 2-4 mm.

8. A method for preparing an organic fertilizer for in situ tea planting according to any one of claims 1 to 7, characterized in that: The steps include: (1): Weigh 10-20 parts of CS-EDTA complex, 5-10 parts of tea waste extract, 30-40 parts of animal waste fermentation product, 10-20 parts of bentonite, and 2.5-5 parts of purified water; (2): The bentonite weighed in step (1) and purified water are stirred and mixed to obtain wet bentonite; (3): The tea waste extract and the animal waste fermentation product weighed in step (1) are stirred and mixed, and the wet bentonite obtained in step (2) is added, and the speed is increased and stirring is continued to obtain an organic fertilizer intermediate product; (4): adding the CS-EDTA complex to the organic fertilizer intermediate product obtained in step (3), stirring and mixing to obtain a wet organic fertilizer; (5): Granulating the wet organic fertilizer obtained in step (4) to obtain organic fertilizer granules; (6): Drying the organic fertilizer particles obtained in step (5) to obtain organic fertilizer for native tea cultivation.

9. The method for preparing an organic fertilizer for native tea planting according to claim 8, characterized in that: The mass ratio of bentonite to purified water in step (1) is 4:1; the stirring time in step (2) is 2 min and the stirring speed is 30 r / min; the mixing process in step (3) is carried out in a three-dimensional mixer; the stirring time of the tea waste extract and the animal waste fermentation product is 3 min and the stirring speed is 50 r / min; during the process of adding wet bentonite, the speed is increased to 100 r / min and the stirring time is 5 min; the stirring speed in step (4) is 80 r / min and the stirring time is 7 min; the granulation process in step (5) uses a granulator, and the size of the organic fertilizer particles is 5±1 mm; the drying method in step (6) is hot air drying, the drying temperature is 70°C, and the drying time is 6 h.

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