Extraction method of pine needle and pinecone extracts

Abstract

The invention relates to an extraction method of pine needle and pinecone extracts, and belongs to the technical field of agriculture. The method comprises the following steps: extracting by using a 20% ethanol solution through an organic solvent extraction method: cleaning fresh pine needles, removing old leaves, air-drying, weighing, cutting to about 2cm, soaking in the 20% ethanol solution overnight, refluxing in a water bath at 60 DEG C for 8 hours, carrying out suction filtration, adding the 20% ethanol solution into sediments, soaking overnight, refluxing in a water bath at 60 DEG C for 8 hours, and carrying out suction filtration. Adding 20% ethanol solution into sediment to soak overnight, refluxing for 8 hours in water bath at 60 DEG C, carrying out suction filtration, combining filtrates obtained in three times, and distilling and concentrating by using a rotary evaporator to obtain extract; the extract can inhibit growth and reproduction of germs, effectively repair plant sick bodies, completely replace fruit growers to scrape and treat diseases, and is labor-saving, time-saving and better in effect compared with manual scraping.

Description

Technical Field

[0001] The invention belongs to the technical field of agriculture and relates to a method for extracting pine needle and pine cone extracts. Background Art

[0002] my country is a big agricultural country with a large area of ​​fruit tree planting. Agriculture has always been a major industry for making people rich. However, the occurrence of fruit tree diseases is a common problem that plagues the development of fruit farmers. For fruit tree diseases such as ring rot, fruit farmers generally use traditional scraping methods to treat the diseases. This treatment method not only has high labor costs and unsatisfactory results, but sometimes also causes damage to the trunks of fruit trees, making it difficult to achieve the desired results.

[0003] Pine needles contain volatile oils, flavonoids, resins, cellulose, lignin, chlorophyll, carotene, vitamins, trace elements and other ingredients. Pine needle extracts are rich in carbohydrates, crude protein, crude fat, various amino acids and various trace minerals, multiple vitamins, bioflavonoids, essential oils, chlorophyll, unsaturated fatty acids, enzymes and coenzymes and other active substances. Pine needle extracts are made into liquid and applied to tree trunks to prevent and control diseases and insect pests. This technology is more effective than manual scraping to treat fruit tree diseases, and can save a lot of labor costs, thereby saving labor, saving time and improving work efficiency. The flavonoids contained in this pine needle extract can also be used as raw materials for animal medicines. Summary of the invention

[0004] In order to effectively extract the extracts of pine needles and pine cones, the present invention adopts the following technical solutions to achieve the extraction method of pine needle and pine cone extracts: 1. Organic solvent extraction method: Use 20% ethanol solution for extraction: Wash fresh pine needles and remove old leaves, air-dry and weigh, cut about 2 cm, soak in 20% ethanol solution overnight, reflux in a 60℃ water bath for 8h, filter, add 20% ethanol solution to the sediment and soak overnight, reflux in a 60℃ water bath for 8h, filter. Add 20% ethanol solution to the sediment and soak overnight, reflux in a 60℃ water bath for 8h, filter, combine the filtrates for 3 times, and distill and concentrate to extract using a rotary evaporator.

[0005] 2. Supercritical extraction method Supercritical CO2 supercritical extraction technology was used to extract terpenes from Pinus tabulaeformis needles. The results showed that the extraction pressure of 15MPa, the extraction temperature of 40℃ and the extraction time of 20min were the extraction conditions with a high yield of terpenes represented by a-pinene and β-caryophyllene from Pinus tabulaeformis needles. Compared with steam distillation, supercritical fluid extraction can preserve a large amount of thermally unstable and easily oxidized components due to its lower operating temperature, maintain the natural quality of the chemical components, and have a high sample yield.

[0006] The beneficial effect of the invention is that the extract can inhibit the growth and reproduction of pathogens, effectively repair plant diseased bodies, completely replace fruit farmers' peeling to treat diseases, save the inefficient manual peeling and poor treatment effect, and achieve the purpose of saving labor and time and better effect than manual peeling. DETAILED DESCRIPTION

[0007] Two extraction methods of pine needle and pine cone extracts:

[0008] 1. Organic solvent extraction method: Use 20% ethanol solution for extraction: Wash fresh pine needles, remove old leaves, air-dry and weigh, cut about 2cm, soak in 20% ethanol solution overnight, reflux in a 60℃ water bath for 8h, filter, add 20% ethanol solution to the sediment overnight, reflux in a 60℃ water bath for 8h, filter. Add 20% ethanol solution to the sediment overnight, reflux in a 60℃ water bath for 8h, filter, combine the filtrates for 3 times, and distill and concentrate to extract using a rotary evaporator.

[0009] 2. Supercritical extraction method Supercritical CO2 supercritical extraction technology was used to extract terpenes from Pinus tabulaeformis needles. The results showed that the extraction pressure of 15MPa, the extraction temperature of 40℃ and the extraction time of 20min were the extraction conditions with a high yield of terpenes represented by a-pinene and β-caryophyllene from Pinus tabulaeformis needles. Compared with steam distillation, supercritical fluid extraction can preserve a large amount of thermally unstable and easily oxidized components due to its lower operating temperature, maintain the natural quality of the chemical components, and have a high sample yield.

[0010] When using, add 50% pine needle and pine cone extract, dilute it with an appropriate amount of quick-acting fertilizer, and apply it to the trunks of fruit trees. It can kill bacteria, viruses and pests, and enable the young tissue inside the diseased area to grow and heal quickly.

[0011] The effect of this extract is obvious. The Wanwu Yangguang brand extract produced by Luohe Nong Baili Technology Co., Ltd. was used for trial in many places in Henan for two consecutive years in 2022 and 2023, and the effect was good. This extract is green organic matter and does not contain pesticides. It is welcomed and praised by local fruit farmers. The flavonoids contained in this pine needle extract can also be used as raw materials for animal drugs, and its market prospects are very promising.

Claims

1. A method for extracting pine needle and pine cone extracts, characterized in that: Organic solvent extraction method: Use 20% ethanol solution for extraction: Wash fresh pine needles, remove old leaves, air-dry and weigh, cut about 2 cm, soak in 20% ethanol solution overnight, reflux in a 60℃ water bath for 8 hours, filter, soak the sediment in 20% ethanol solution overnight, reflux in a 60℃ water bath for 8 hours, filter. Soak the sediment in 20% ethanol solution overnight, reflux in a 60℃ water bath for 8 hours, filter, combine the filtrates for three times, and distill and concentrate to extract using a rotary evaporator.

2. A method for extracting pine needle and pine cone extracts, characterized in that: Supercritical CO2 extraction was used to extract terpenes from Pinus tabulaeformis needles. The results showed that the extraction pressure of 15 MPa, the extraction temperature of 40 °C and the extraction time of 20 min were the extraction conditions with a high yield of terpenes represented by a-pinene and β-caryophyllene from Pinus tabulaeformis needles.