Bactericidal composition for preventing and treating anthracnose of carambola

Abstract

The invention belongs to the technical field of bactericides, and particularly relates to a bactericidal composition for preventing and treating carambola anthracnose. The invention relates to a bactericidal composition. The bactericidal composition is prepared by binary compounding of pyrazamine, orysastrobin, sedaxane or berberine, wherein the mass ratio of the pyrazamide to the orysastrobin is (1-16): (32-1); the mass ratio of the pyrazamide to the sedaxane is 1: (12-1); the mass ratio of the tetrazopiramate to the berberine is (1-20): (4-1). According to the invention, a hypha growth rate method is adopted to determine the combined action type of the compounding of the pyrazolostrobin and the existing bactericidal active component on the colletotrichum gloeosporioides, and it is found that the pyrazolostrobin and orysastrobin, sedaxane or berberine have a synergistic effect on inhibition of the growth of the hypha of the colletotrichum gloeosporioides under the condition of different mass ratios. Compared with a single component, the composition can improve the prevention and treatment effect on the carambola anthracnose, can reduce the pesticide dosage and reduce the pesticide residue, and is beneficial to green, healthy and sustainable development of the carambola industry.

Description

Technical Field

[0001] The invention belongs to the technical field of fungicides, and particularly relates to a fungicide composition for preventing and treating carambola anthracnose. Background Art

[0002] Carambola belongs to the genus Acaromyces of the family Solanaceae. It can bloom and bear fruit many times a year and has high economic value. During the growth and development of carambola, it will be harmed by various diseases such as anthracnose and red spot. Among them, carambola anthracnose can be caused by infection with Colletotrichum gloeosporioides, which mainly harms the fruit. The affected fruit is easy to fall off. It often occurs on nearly mature or mature fruits. Fruits with wounds are also susceptible to infection during storage and transportation. In the early stage of the disease, there are dark brown round or nearly round spots on the surface of the fruit. In the later stage, the spots are black. The diseased tissue can expand and penetrate into the inside of the fruit, causing the whole fruit to rot.

[0003] In the process of agricultural disease prevention and control, disease resistance is an issue that cannot be ignored. Compounding different active ingredients is a common method for preventing and controlling resistant diseases. When different active ingredients are compounded, they usually show three types of combined effects: synergistic effect, additive effect and antagonistic effect. In the vast majority of cases, compound combinations mostly show additive effects, and there are very few compound combinations that truly have synergistic effects, especially those with very obvious synergistic effects.

[0004] Picarbutrazox is a carbamate fungicide and the only compound in tetrazolyl oxime fungicide. CAS registration number: 500207-04-5, molecular formula: C 20 H 23 N7O3, relative molecular mass: 409.442. The mechanism of action of tetrazolylpyridine is the U17 category with unknown mechanism of action in the FRAC coding table, but it does not inhibit complex I or complex III of the respiratory chain electron transport system, and has no cross-resistance with QoI and benzamide fungicides. Tetrazolylpyridine can prevent and control a variety of fungal diseases of crops by inhibiting the formation of bacterial cell membranes, inhibiting hyphae growth and spore germination, and reducing the number of sporangia and zoospores.

[0005] The inventors used the hyphae growth rate method to determine the type of combined action of tetrazolylpyram and existing fungicidal active ingredients on carambola anthracnose, and found that tetrazolylpyram and orysastrobin, fluazifop or berberine showed a synergistic effect on inhibiting the hyphae growth of carambola anthracnose at different mass ratios. No relevant combination reports have been seen so far. Summary of the invention

[0006] The object of the present invention is to provide a fungicide composition for preventing and treating carambola anthracnose, wherein the compound combination thereof exhibits a synergistic effect on inhibiting the mycelial growth of carambola anthracnose fungi.

[0007] To achieve the above object, the present invention provides the following technical solutions:

[0008] A bactericidal composition, which is formed by a binary compound of tetrazolium pyridine and orysastrobin, fluazifop or berberine; wherein the mass ratio of tetrazolium pyridine to orysastrobin is 1-16:32-1; the mass ratio of tetrazolium pyridine to fluazifop is 1:12-1; and the mass ratio of tetrazolium pyridine to berberine is 1-20:4-1.

[0009] Furthermore, the mass ratio of tetrazolium pyrazone to fluazifop is 1:8.

[0010] The invention also provides application of the bactericidal composition in preventing and treating carambola anthracnose.

[0011] The present invention also provides a bactericide, which comprises auxiliary components and the bactericidal composition.

[0012] Furthermore, the mass of the bactericidal composition accounts for 3-75% of the total mass of the bactericide.

[0013] Compared with the prior art, the present invention has the following beneficial effects:

[0014] (1) The present invention uses the mycelium growth rate method to determine the combined action type of tetrazolylpyram and the existing fungicidal active ingredients on carambola anthracnose, and finds that tetrazolylpyram and orysastrobin, fluazifop or berberine show a synergistic effect on inhibiting the mycelial growth of carambola anthracnose at different mass ratios. Compared with a single ingredient, the control effect on carambola anthracnose can be improved, the amount of pesticides used can be reduced, pesticide residues can be reduced, environmental pollution can be alleviated, and the green, healthy and sustainable development of the carambola industry can be contributed to.

[0015] (2) The bactericidal composition of the present invention is composed of two bactericidal active ingredients. Compared with a single ingredient, it can reduce the risk of drug resistance and extend the service life of the agent. DETAILED DESCRIPTION

[0016] The technical solution of the present invention is clearly and completely described below in conjunction with the embodiments.

[0017] Example

[0018] 1. Test strains

[0019] Colletotrichum gloeosporioides, isolated from carambola fruits showing symptoms of anthracnose.

[0020] 2. Test reagents

[0021] 97% tetrazolium pyrazone technical, 98% orysastrobin technical, 95% fluazifop technical, 98% berberine technical, all of the above agents are commercially available.

[0022] 3. Test methods

[0023] The mycelial growth rate method was used to determine the toxicity of tetrazolium pyraclostrobin, orysastrobin, fluazifop and berberine alone and their mixtures against the carambola anthracnose pathogen Colletotrichum gloeosporioides.

[0024] The test agent was dissolved in methyl sulfoxide and then diluted with a 0.1% Tween-80 aqueous solution to prepare a single-dose stock solution with an active ingredient concentration of 1000 mg / L. The single dose was diluted with a 0.1% Tween-80 aqueous solution in an equal proportion to form 5 mass concentration gradients, and then the toxicity of the single dose to the carambola anthracnose pathogen Colletotrichum gloeosporioides was determined.

[0025] On the basis of the single-dose toxicity determination, multiple groups of mixtures were set up, and each group of mixtures was diluted into 5 mass concentration gradients with 0.1% Tween-80 aqueous solution in equal proportions, and then the toxicity of each mixture to the carambola anthracnose pathogen Colletotrichum gloeosporioides was determined.

[0026] The specific method for the toxicity determination of single doses and mixed doses is as follows: pre-melted PDA medium and the drug solution to be tested are mixed evenly in a conical flask at a volume ratio of 9:1, and then poured into a 9 cm diameter culture dish, 10 mL per dish, and the drug-containing plate is formed after the PDA medium is cooled; and the treatment of 0.1% Tween-80 aqueous solution is set as a blank control. Five plates are set for each treatment.

[0027] The test strain was propagated and cultured on PDA medium at 25°C for 4 days, and then a 6mm diameter bacterial cake was punched at the edge of the test strain colony with a hole puncher. The bacterial cake was inoculated in the center of the drug-containing plate and the blank control plate, with one bacterial cake per plate. The plates were covered and cultured at 25°C. When the diameter of the blank control colony grew to about 6cm, the colony diameter was measured by the cross method, and the mycelium growth inhibition rate of different treatments was calculated. A linear regression was performed with the logarithm of the drug concentration as x and the probability value of the mycelium growth inhibition rate as y to obtain the toxicity regression equation and the toxicity EC of the drug to the test strain. 50 value.

[0028]

[0029] 4. Data Analysis

[0030] The co-toxicity coefficient (CTC) was calculated using the Sun Yunpei co-toxicity coefficient method, and the type of combined action of the compound combination on the test strain was evaluated based on the co-toxicity coefficient value: CTC≤80 was antagonistic, 80<CTC<120 was additive, and CTC≥120 was synergistic. The results are shown in Tables 1-3.

[0031] Table 1 The results of toxicity test of tetrazolium pyrazone and orysastrobin against Colletotrichum gloeosporioides

[0032]

[0033] As shown in Table 1, within the mass ratio range of 1-16:32-1, the combination of tetrazolylpyram and orysastrobin shows a synergistic effect in inhibiting the mycelial growth of carambola anthracnose fungi (Colletotrichum gloeosporioides). This indicates that the fungicidal composition prepared by combining tetrazolylpyram and orysastrobin in the present invention can improve the control effect of carambola anthracnose, reduce the amount of pesticides used, reduce pesticide residues, and alleviate environmental pollution.

[0034] Table 2 The results of the toxicity test of tetrazolium pyrazone and fluazifop-butyl against Colletotrichum gloeosporioides

[0035]

[0036]

[0037] As shown in Table 2, within the mass ratio range of 1:12-1, the combination of tetrazolylpyram and fluzopyram exhibits a synergistic effect in inhibiting the mycelial growth of carambola anthracnose fungi (Colletotrichum gloeosporioides). This indicates that the fungicidal composition prepared by combining tetrazolylpyram and fluzopyram can improve the control effect of carambola anthracnose, reduce the amount of pesticides used, reduce pesticide residues, and alleviate environmental pollution.

[0038] Table 3 The results of the toxicity test of tetrazolium pyrazone and berberine against Colletotrichum gloeosporioides

[0039]

[0040] As shown in Table 3, within the mass ratio range of 1-20:4-1, the combination of tetrazolium pyrrolidone and berberine showed a synergistic effect in inhibiting the mycelial growth of carambola anthracnose fungi (Colletotrichum gloeosporioides). This indicates that the fungicidal composition formed by the combination of tetrazolium pyrrolidone and berberine of the present invention can improve the control effect of carambola anthracnose, reduce the amount of pesticides used, reduce pesticide residues, and alleviate environmental pollution.

[0041] In summary, the present invention uses the mycelium growth rate method to determine the combined action type of tetrazolylpyram and the existing fungicidal active ingredients on carambola anthracnose, and finds that tetrazolylpyram and oxazolidinone, fluazifop or berberine show a synergistic effect on inhibiting the mycelial growth of carambola anthracnose at different mass ratios. Compared with a single ingredient, the control effect of carambola anthracnose can be improved, the amount of pesticides used can be reduced, pesticide residues can be reduced, environmental pollution can be alleviated, and the green, healthy and sustainable development of the carambola industry can be contributed to.

Claims

1. A bactericidal composition, characterized in that: The bactericidal composition is a binary compound of tetrazolium pyrazone and orysastrobin, fluazifop or berberine; wherein, The mass ratio of tetrazolium pyrazone to orysastrobin is 1-16:32-1; The mass ratio of tetrazolium pyrazone to fluazifop is 1:12-1; The mass ratio of tetrazolium pyrrolidone to berberine is 1-20:4-1.

2. The bactericidal composition according to claim 1, characterized in that: The mass ratio of tetrazolium pyrazone to fluazifop is 1:

8.

3. Use of the fungicide composition according to any one of claims 1 to 2 in preventing and treating carambola anthracnose.

4. A bactericide, characterized in that: The bactericide comprises auxiliary components and the bactericidal composition according to claim 1.

5. The fungicide according to claim 1, characterized in that The mass of the bactericidal composition accounts for 3-75% of the total mass of the bactericide.

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