Pleurotus geesteranus culture medium and preparation method and culture method thereof
By adding plant-source insecticides such as bitter vine rhizome, fennel and orange peel to the Xiuzhen mushroom cultivation substrate, the problem of failure to effectively prevent and control pests in the existing technology is solved, and the effect of high yield and healthy growth is achieved, and a green and environmentally friendly method is adopted.
Patent Information
- Application Number
- CN202411895286.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-21
- Publication Date
- 2025-02-25
AI Technical Summary
The prior art only considers the cost reduction when preparing the Xiuzhen mushroom culture medium, and does not involve how to control pests in green during the cultivation process.
The cultivation matrix of Xiuzhen mushrooms consisting of lemon strips, cottonseed shells, lime powder, bitter skin vine rhizomes, fennel, orange peel and corn cob is used to effectively prevent and control pests through the plant-source insecticide effect of bitter skin vine rhizomes, fennel and orange peel.
This method not only increases the yield of Xiuzhen mushrooms, but also ensures the healthy growth of fruiting entities, avoids the potential harm caused by the use of pesticides, and achieves a green and environmentally friendly pest control effect.
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Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of edible fungus cultivation, and in particular relates to a Pleurotus geesteranus cultivation substrate and a preparation method and a cultivation method thereof. Background Art
[0002] Pleurotus geesteranus is a kind of mushroom that grows in tropical and subtropical regions. It is also known as pocket mushroom, ring-stalked pleurotus, yellow-white pleurotus, ring-stalked fighting mushroom, pleurotus ostreatus and small pleurotus. Pleurotus geesteranus is not only tender and crispy, low in fiber, with a great taste and delicious taste, but also rich in nutrition, with nutritional value equivalent to milk. The polysaccharides it contains have been proven to have anti-tumor functions. Its fruiting bodies are rich in high-quality bacterial proteins and 17 kinds of amino acids and various trace elements required by the human body. It is a rare edible fungus with extremely high nutritional value. At the same time, because Pleurotus geesteranus is a high-temperature edible fungus, it needs to be cultivated under facility conditions in the northwest region, and the environmental factors such as temperature, humidity, carbon dioxide, and light need to be regulated for normal growth.
[0003] Pleurotus geesteranus is rich in protein, fungal polysaccharides, vitamins and various trace elements. It is not only nutritious but also has health functions. The protein content of Pleurotus geesteranus is close to that of meat, and is 3 to 6 times higher than that of ordinary vegetables. Pleurotus geesteranus contains threonine, lysine, leucine, etc., which cannot be produced by the human body and are very lacking in the daily diet. The proteoglycans contained in Pleurotus geesteranus have a strong inhibitory effect on cancer cells and can enhance the body's immune function. Regular consumption of Pleurotus geesteranus can not only improve human metabolism and regulate autonomic nerves, but also has obvious effects on reducing human serum cholesterol, lowering blood pressure, preventing hepatitis, gastric ulcers and duodenal ulcers. It can be seen that Pleurotus geesteranus is a high-protein and low-fat healthy food.
[0004] In the prior art, when preparing a culture substrate for Pleurotus geesteranus, only the cost of cultivating Pleurotus geesteranus is considered. For example, Chinese patent CN112293162A discloses a straw culture substrate and a Pleurotus geesteranus cultivation method, which include the following raw materials in weight percentage: 0-25% corn straw, 40%-50% corn cob, 0-25% rice straw, 0-30% bean straw, 15%-25% bran, 5%-10% soybean meal, 1.0%-2.0% lime powder, 2.0%-2.5% gypsum powder and 0.3%-0.5% potassium dihydrogen phosphate. For another example, Chinese patent CN117694177A shows that the existing Pleurotus geesteranus culture substrate only considers reducing the cost of cultivating Pleurotus geesteranus, and does not involve how to prevent and control pests during the cultivation of Pleurotus geesteranus. Summary of the invention
[0005] The invention discloses a Pleurotus geesteranus cultivation substrate and a preparation method and a cultivation method thereof, which solve the problem that in the prior art, when preparing a Pleurotus geesteranus cultivation substrate, only the cost of cultivating Pleurotus geesteranus is reduced, but how to carry out green pest control in the process of cultivating Pleurotus geesteranus is not involved.
[0006] A cultivation substrate for Pleurotus geesteranus consists of the following raw materials in parts by weight: 60-80 parts of Caragana korshinskii, 20-40 parts of cottonseed hulls, 1-5 parts of lime powder, 6-12 parts of smilax glabra rhizomes, 3-9 parts of fennel, 10-17 parts of orange peels and 15-20 parts of corn cobs.
[0007] Preferably, it is composed of the following raw materials in parts by weight: 65 to 75 parts of Caragana korshinskii, 25 to 35 parts of cottonseed hulls, 2 to 4 parts of lime powder, 8 to 10 parts of Caulis davidiana rhizomes, 5 to 7 parts of fennel, 12 to 14 parts of orange peels, and 16 to 18 parts of corn cobs.
[0008] Preferably, it is composed of the following raw materials in parts by weight: 70 parts of Caragana korshinskii, 30 parts of cottonseed hulls, 3 parts of lime powder, 9 parts of Cistanche deserticola rhizomes, 6 parts of fennel, 13 parts of orange peels, and 17 parts of corn cobs.
[0009] The second object of the present invention is to protect the preparation method of the Pleurotus geesteranus cultivation substrate, which specifically comprises the following steps:
[0010] Crush the formulated amount of Caragana korshinskii to obtain Caragana korshinskii powder;
[0011] Grinding corn cobs into pieces of 5 mm to 8 mm to obtain corn cobs;
[0012] The preparation methods of Caulis Achyranthis Bidentatae rhizome powder, fennel powder and orange peel powder are the same as that of Caragana korshinskii powder;
[0013] Soaking a formulated amount of cottonseed hulls in water until the cottonseed hulls no longer absorb water, thereby obtaining cottonseed hulls that have absorbed water;
[0014] The lime powder, prepared Caragana korshinskii powder, Caulis davidiana root and stem powder, fennel powder, orange peel powder, corn cob and cottonseed hulls after absorbing water are mixed evenly, the water content is adjusted to 50% to 60%, and the Pleurotus geesteranus cultivation medium is obtained by sterilization.
[0015] Preferably, the caragana korshinskii is crushed to 1 mm to 2 mm.
[0016] Preferably, the sterilization condition is 120°C to 122°C for 110 min to 120 min.
[0017] The third object of the present invention is to protect a method for cultivating Pleurotus geesteranus using the Pleurotus geesteranus cultivation substrate, which specifically comprises the following steps:
[0018] Making mushroom bags: bagging and sterilizing the prepared Pleurotus geesteranus cultivation substrate to obtain sterilized mushroom bags;
[0019] Inoculation: Inoculate the sterilized fungus bag to obtain an inoculated fungus bag;
[0020] Mycelium management: Cultivate the inoculated bacteria bags to obtain bacteria sticks with full mycelium;
[0021] Fruiting management: Move the mushroom sticks after full mycelium growth into the mushroom shed for fruiting cultivation;
[0022] Harvesting: Start harvesting when the fruiting bodies of Pleurotus geesteranus grow to 60% to 70%.
[0023] Preferably, during inoculation, the inoculation amount is 10 mg / g to 20 mg / g.
[0024] Preferably, during mushroom production management, the temperature of the mushroom shed is 22°C to 25°C and the relative humidity is 80% to 90%.
[0025] Compared with the prior art, the present invention has the following beneficial effects:
[0026] The cultivation substrate of Pleurotus geesteranus prepared by the present invention comprises 60 to 80 parts of Caragana korshinskii, 20 to 40 parts of cottonseed shells, 1 to 5 parts of lime powder and 15 to 20 parts of corn cobs, and 6 to 12 parts of Cistanche deserticola rhizomes, 3 to 9 parts of fennel and 10 to 17 parts of orange peels are added. Cistanche deserticola rhizomes, fennel and orange peels are all plant-derived insecticides, which can effectively prevent and control pests, increase yields and have no effect on the fruiting bodies of Pleurotus geesteranus. Cistanche deserticola rhizomes have a killing effect on pests in the growth process of Pleurotus geesteranus and are plant-derived insecticides and sterilizers; anisaldehyde, which is rich in fennel, is also an agricultural insecticide; and orange peels have a controlling effect on springtails.
[0027] In the matrix of comparative example 2 of the present application, fennel and orange peel were not added, only bitter vine was added, which had low lethality to pests, and the pests nibbled on the fruiting bodies of Pleurotus geesteranus, resulting in a reduction in the yield of Pleurotus geesteranus. In the matrix of comparative example 3, orange peel was not added, and the control ability of springtails was weakened, resulting in a reduction in the yield of Pleurotus geesteranus. In the matrix of comparative example 4, bitter vine was not added, and the control ability of pests was weak, resulting in a reduction in the yield of Pleurotus geesteranus. When pesticides and insecticides were used, Pleurotus geesteranus was also in a state of reduced yield. Only when the raw materials of the present invention cooperated with each other, the yield was greatly improved, the growth of the fruiting bodies was also greatly improved, and there was significant progress. This effect was based on the interference of fennel with the nervous system of pests, the volatility in orange peel The hair oil interferes with the olfactory system of pests, preventing them from finding food or mates. On the basis of destroying their mobility, the rhizomes of Caulis Agaricus serrata have a feeding repellent effect on pests and play an insecticidal role when the vitality of the pests is weak, thereby improving the pest control effect. When the amount of fennel and orange peel exceeds the dosage range of the present invention, their strong aroma will repel the pests in a short time, but after the repellent, the pests will again cause a reduction in the yield of Pleurotus geesteranus, and the insecticidal effect cannot be achieved. Therefore, within the dosage range of the present invention, the insecticidal effect and nutritional components are taken into account, and the purpose of preventing and controlling pests and ensuring the yield of Pleurotus geesteranus is achieved. It can be seen that the raw materials of this application cannot be arbitrarily deleted or replaced.
[0028] Caragana powder is added to the Pleurotus geesteranus cultivation substrate prepared by the invention, and the caragana powder can improve the material structure of the cultivation substrate and is beneficial to the growth of Pleurotus geesteranus hyphae. The use of corn cobs increases the air contact amount and is beneficial to the growth of hyphae. DETAILED DESCRIPTION
[0029] The technical solutions in the present invention will be described clearly and completely below in conjunction with the specific implementation of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.
[0030] The methods described in the embodiments of the present invention are conventional methods unless otherwise specified. The materials, reagents, etc. used can be obtained from commercial sources unless otherwise specified.
[0031] Example 1
[0032] A cultivation medium for Pleurotus geesteranus consists of the following raw materials in parts by weight: 60 parts of Caragana korshinskii, 20 parts of cottonseed hulls, 1 part of lime powder, 6 parts of smilax glabra rhizomes, 3 parts of fennel, 10 parts of orange peels and 15 parts of corn cobs.
[0033] A method for preparing a cultivation substrate for Pleurotus geesteranus, comprising the following steps:
[0034] The formulated amount of Caragana korshinskii was crushed into 1 mm to obtain Caragana korshinskii powder.
[0035] The corn cobs in the formula amount are crushed into 5 mm to obtain corn cobs.
[0036] The formulated amount of Celastrus angulatus rhizome is crushed into 1 mm to obtain Celastrus angulatus rhizome powder.
[0037] Grind the formulated amount of fennel into 1 mm to obtain fennel powder.
[0038] The formulated amount of orange peel is crushed into 1 mm to obtain orange peel powder.
[0039] Soak a formulated amount of cottonseed hulls in water until the cottonseed hulls no longer absorb water, thereby obtaining the water-absorbed cottonseed hulls.
[0040] The lime powder, prepared Caragana korshinskii powder, Caulis davidiana root and stem powder, fennel powder, orange peel powder, corn cob and cottonseed hulls after absorbing water in the prescribed amount are uniformly mixed, the water content is adjusted to 50%, and sterilized at 120° C. for 30 minutes to obtain the Pleurotus geesteranus cultivation medium.
[0041] A method for cultivating Pleurotus geesteranus using the Pleurotus geesteranus cultivation substrate specifically comprises the following steps:
[0042] Preparation of mushroom bags: bag the prepared Pleurotus geesteranus cultivation substrate in a bagging amount of 750 g / bag, sterilize at 120°C for 30 min, and obtain sterilized mushroom bags.
[0043] Inoculation: Inoculate the sterilized bags with an inoculation amount of 10mg / g. The specific operation method is: move the sterilized bags into the sterilized inoculation room for natural cooling, and inoculate when the bag temperature is 23°C. Before inoculation, put the inoculation tools, collars, plastic covers, and alcohol cotton balls into the inoculation room and fumigate with sodium dichloroisocyanurate, 10g per cubic meter. During inoculation, the operator wipes and disinfects the hands and the surface of the cultivated species with cotton balls. Two people operate, one is responsible for uncovering and covering the cover, and the other is responsible for inoculation. One end is inoculated, and the cultivated species connected seals the surface of the material to obtain an inoculated bag.
[0044] Management of mushroom production: The temperature is controlled at 28℃, and the contaminated mushroom bags are removed in time. When the mycelium has eaten half of the material, turn the bag over once. When the mycelium is fully grown and there is a hollow drum sound when knocking, it can be stacked, and the mushroom stick production is completed.
[0045] Management of mushroom production: Cultivate the inoculated mushroom bags, and move them into the mushroom shed for mushroom production after the mycelium is fully grown. First, open the air outlet membrane of the mushroom shed, lower the ambient temperature of the mushroom shed to 8℃, stimulate at low temperature for 12 hours, and spray humidification into the space regularly to keep the relative humidity at 85% to stimulate the mycelium to form primordia. After most of the primordia appear, carry out mushroom production in the shed, remove the mushroom cap and collar at the same time, do not spray water, ventilate 8 times a day, 15 minutes each time, control the relative humidity of the air between 80%, and provide scattered light, so that the mycelium can produce mushrooms as soon as possible under the stimulation of low temperature and light.
[0046] Harvesting: When the fruiting bodies of Pleurotus geesteranus grow to 60% to 70%, that is, the cap grows to 2cm, the edge of the cap curls inward, and the color gradually changes from dark to light, start harvesting before the spores are ejected.
[0047] Example 2
[0048] A cultivation medium for Pleurotus geesteranus consists of the following raw materials in parts by weight: 65 parts of Caragana korshinskii, 25 parts of cottonseed hulls, 2 parts of lime powder, 8 parts of smilax glabra rhizomes, 5 parts of fennel, 12 parts of orange peels and 16 parts of corn cobs.
[0049] A method for preparing a cultivation substrate for Pleurotus geesteranus, comprising the following steps:
[0050] The formulated amount of Caragana korshinskii was crushed into 1 mm to obtain Caragana korshinskii powder.
[0051] The corn cobs in the formula amount are crushed into 6 mm to obtain corn cobs.
[0052] The formulated amount of Celastrus angulatus rhizome is crushed into 1 mm to obtain Celastrus angulatus rhizome powder.
[0053] Grind the formulated amount of fennel into 1 mm to obtain fennel powder.
[0054] The formulated amount of orange peel is crushed into 1 mm to obtain orange peel powder.
[0055] Soak the cottonseed hulls in water until the cottonseed hulls no longer absorb water, thereby obtaining the cottonseed hulls after absorbing water.
[0056] The lime powder, prepared Caragana korshinskii powder, Caulis davidiana root and stem powder, fennel powder, orange peel powder, corn cob and cottonseed hulls after absorbing water in the formulated amount are uniformly mixed, the water content is adjusted to 50%, and sterilized at 120° C. for 30 minutes to obtain the Pleurotus geesteranus cultivation medium.
[0057] A method for cultivating Pleurotus geesteranus using the Pleurotus geesteranus cultivation substrate specifically comprises the following steps:
[0058] Preparation of mushroom bags: bag the prepared Pleurotus geesteranus cultivation substrate in a bagging amount of 450 g / bag, sterilize at 120°C for 30 min, and obtain sterilized mushroom bags.
[0059] Inoculation: Inoculate the sterilized bags with an inoculation amount of 10mg / g. The specific operation method is: move the sterilized bags into the sterilized inoculation room for natural cooling, and inoculate when the bag temperature is 23°C. Before inoculation, put the inoculation tools, collars, plastic covers, and alcohol cotton balls into the inoculation room and fumigate with sodium dichloroisocyanurate, 10g per cubic meter. During inoculation, the operator wipes and disinfects the hands and the surface of the cultivated species with cotton balls. Two people operate, one is responsible for uncovering and covering the cover, and the other is responsible for inoculation. One end is inoculated, and the cultivated species connected seals the surface of the material to obtain an inoculated bag.
[0060] Management of mushroom production: The temperature is controlled at 28℃, and the contaminated mushroom bags are removed in time. When the mycelium has eaten half of the material, turn the bag over once. When the mycelium is fully grown and there is a hollow drum sound when knocking, it can be stacked, and the mushroom stick production is completed.
[0061] Management of mushroom production: Cultivate the inoculated mushroom bags, and move them into the mushroom shed for mushroom production after the mycelium is fully grown. First, open the air outlet membrane of the mushroom shed, lower the ambient temperature of the mushroom shed to 10℃, stimulate at low temperature for 12 hours, and spray humidification into the space regularly to keep the relative humidity at 90% to stimulate the mycelium to form primordia. After most of the primordia appear, the shed can be sealed for mushroom production. At the same time, the mushroom cap and the collar can be removed. Water cannot be sprayed. Ventilate 8 times a day, 15 minutes each time, control the relative humidity of the air at 85%, and provide scattered light to make the mycelium produce mushrooms as soon as possible under the stimulation of low temperature and light.
[0062] Harvesting: When the fruiting bodies of Pleurotus geesteranus grow to 60% to 70%, that is, the cap grows to 2-3cm, the edge of the cap curls inward, and the color gradually changes from dark to light, start harvesting before the spores are ejected.
[0063] Example 3
[0064] A cultivation medium for Pleurotus geesteranus consists of the following raw materials in parts by weight: 70 parts of Caragana korshinskii, 30 parts of cottonseed hulls, 3 parts of lime powder, 9 parts of smilax glabra rhizomes, 6 parts of fennel, 13 parts of orange peels and 17 parts of corn cobs.
[0065] A method for preparing a cultivation substrate for Pleurotus geesteranus, comprising the following steps:
[0066] The formulated amount of Caragana korshinskii was crushed into 2 mm to obtain Caragana korshinskii powder.
[0067] The corn cobs in the formula amount are crushed into 7 mm to obtain corn cobs.
[0068] The formulated amount of Celastrus angulatus rhizome is crushed into 2 mm to obtain Celastrus angulatus rhizome powder.
[0069] Grind the formulated amount of fennel into 2 mm to obtain fennel powder.
[0070] The formulated amount of orange peel is crushed into 2 mm to obtain orange peel powder.
[0071] Soak a formulated amount of cottonseed hulls in water until the cottonseed hulls no longer absorb water, thereby obtaining the water-absorbed cottonseed hulls.
[0072] The lime powder, prepared Caragana korshinskii powder, Caulis davidiana root and stem powder, fennel powder, orange peel powder, corn cob and cottonseed hulls after absorbing water in the formulated amount are uniformly mixed, the water content is adjusted to 55%, and sterilized at 121° C. for 35 minutes to obtain the Pleurotus geesteranus cultivation medium.
[0073] A method for cultivating Pleurotus geesteranus using the Pleurotus geesteranus cultivation substrate specifically comprises the following steps:
[0074] Preparation of mushroom bags: bag the prepared Pleurotus geesteranus cultivation substrate in a bagging amount of 450 g / bag, sterilize at 121° C. for 35 min, and obtain sterilized mushroom bags.
[0075] Inoculation: Inoculate the sterilized bags with an inoculation amount of 15 mg / g. The specific operation method is: move the sterilized bags into the sterilized inoculation room for natural cooling, and inoculate when the bag temperature is 24°C. Before inoculation, put the inoculation tools, collars, plastic covers, and alcohol cotton balls into the inoculation room and fumigate with sodium dichloroisocyanurate, 10g per cubic meter. During inoculation, the operator wipes and disinfects the hands and the surface of the cultivated species with cotton balls. Two people operate, one is responsible for uncovering and covering the cover, and the other is responsible for inoculation. One end is inoculated, and the cultivated species connected seals the surface of the material to obtain an inoculated bag.
[0076] Management of mushroom growth: The temperature is controlled at 27℃, and the contaminated mushroom bags are removed in time. When the mycelium has eaten half of the material, turn the bag over once. When the mycelium is fully grown and there is a hollow drum sound when knocking, it can be stacked, and the mushroom stick production is completed.
[0077] Management of mushroom production: Cultivate the inoculated mushroom bags, and move them into the mushroom shed for mushroom production after the mycelium is fully grown. First, open the air outlet membrane of the mushroom shed, lower the ambient temperature of the mushroom shed to 12℃, stimulate at low temperature for 13 hours, and spray humidification into the space regularly to keep the relative humidity at 95% to stimulate the mycelium to form primordia. After most of the primordia appear, carry out mushroom production in the shed, remove the mushroom cap and collar at the same time, do not spray water, ventilate 9 times a day, 15 minutes each time, control the relative humidity of the air at 90%, and provide scattered light to make the mycelium produce mushrooms as soon as possible under the stimulation of low temperature and light.
[0078] Harvesting: When the fruiting bodies of Pleurotus geesteranus grow to 60% to 70%, that is, the cap grows to 2-3cm, the edge of the cap curls inward, and the color gradually changes from dark to light, start harvesting before the spores are ejected.
[0079] Example 4
[0080] A cultivation medium for Pleurotus geesteranus consists of the following raw materials in parts by weight: 75 parts of Caragana korshinskii, 35 parts of cottonseed hulls, 4 parts of lime powder, 10 parts of smilax glabra rhizomes, 7 parts of fennel, 14 parts of orange peels and 18 parts of corn cobs.
[0081] A method for preparing a cultivation substrate for Pleurotus geesteranus, comprising the following steps:
[0082] The formulated amount of Caragana korshinskii was crushed into 2 mm to obtain Caragana korshinskii powder.
[0083] The corn cobs in the formula amount are crushed into 5 mm to obtain corn cobs.
[0084] The formulated amount of Celastrus angulatus rhizome is crushed into 2 mm to obtain Celastrus angulatus rhizome powder.
[0085] Grind the formulated amount of fennel into 2 mm to obtain fennel powder.
[0086] The formulated amount of orange peel is crushed into 2 mm to obtain orange peel powder.
[0087] Soak a formulated amount of cottonseed hulls in water until the cottonseed hulls no longer absorb water, thereby obtaining the water-absorbed cottonseed hulls.
[0088] The lime powder, prepared Caragana korshinskii powder, Caulis davidiana root and stem powder, fennel powder, orange peel powder, corn cob and cottonseed hulls after absorbing water in the prescribed amount are uniformly mixed, the water content is adjusted to 60%, and sterilized at 122° C. for 40 minutes to obtain the Pleurotus geesteranus cultivation medium.
[0089] A method for cultivating Pleurotus geesteranus using the Pleurotus geesteranus cultivation substrate specifically comprises the following steps:
[0090] Preparation of mushroom bags: bag the prepared Pleurotus geesteranus cultivation substrate in a bagging amount of 450 g / bag, sterilize at 122°C for 40 min, and obtain sterilized mushroom bags.
[0091] Inoculation: Inoculate the sterilized bags with an inoculation amount of 20 mg / g. The specific operation method is: move the sterilized bags into the sterilized inoculation room for natural cooling, and inoculate when the bag temperature is 25°C. Before inoculation, put the inoculation tools, rings, plastic covers, and alcohol cotton balls into the inoculation room and fumigate with sodium dichloroisocyanurate, 10g per cubic meter. During inoculation, the operator wipes and disinfects the hands and the surface of the cultivated species with cotton balls. Two people operate, one is responsible for uncovering and covering the cover, and the other is responsible for inoculation. One end is inoculated, and the cultivated species connected seals the surface of the material to obtain an inoculated bag.
[0092] Management of mushroom production: The temperature is controlled at 26℃, and the contaminated mushroom bags are removed in time. When the mycelium has eaten half of the material, turn the bag over once. When the mycelium is fully grown and there is a hollow drum sound when knocking, it can be stacked, and the mushroom stick production is completed.
[0093] Management of mushroom production: Cultivate the inoculated mushroom bags, and move them into the mushroom shed for mushroom production after the mycelium is fully grown. First, open the air outlet membrane of the mushroom shed, lower the ambient temperature of the mushroom shed to 13℃, stimulate at low temperature for 14 hours, and spray humidification into the space regularly to keep the relative humidity at 95% to stimulate the mycelium to form primordia. After most of the primordia appear, the shed can be sealed for mushroom production. At the same time, the mushroom cap and the collar can be removed. Water cannot be sprayed. Ventilate 10 times a day, 15 minutes each time, control the relative humidity of the air at 95%, and provide scattered light to make the mycelium produce mushrooms as soon as possible under the stimulation of low temperature and light.
[0094] Harvesting: When the fruiting bodies of Pleurotus geesteranus grow to 60% to 70%, that is, the cap grows to 3cm, the edge of the cap curls inward, and the color gradually changes from dark to light, start harvesting before the spores are ejected.
[0095] Example 5
[0096] A cultivation medium for Pleurotus geesteranus consists of the following raw materials in parts by weight: 80 parts of Caragana korshinskii, 40 parts of cottonseed hulls, 5 parts of lime powder, 12 parts of smilax glabra rhizomes, 9 parts of fennel, 17 parts of orange peels and 20 parts of corn cobs.
[0097] A method for preparing a cultivation substrate for Pleurotus geesteranus, comprising the following steps:
[0098] The formulated amount of Caragana korshinskii was crushed into 2 mm to obtain Caragana korshinskii powder.
[0099] The corn cobs in the formula amount are crushed into 8 mm to obtain corn cobs.
[0100] The formulated amount of Celastrus angulatus rhizome is crushed into 2 mm to obtain Celastrus angulatus rhizome powder.
[0101] Grind the formulated amount of fennel into 2 mm to obtain fennel powder.
[0102] The formulated amount of orange peel is crushed into 2 mm to obtain orange peel powder.
[0103] Soak a formulated amount of cottonseed hulls in water until the cottonseed hulls no longer absorb water, thereby obtaining the water-absorbed cottonseed hulls.
[0104] The lime powder, prepared Caragana korshinskii powder, Caulis davidiana root and stem powder, fennel powder, orange peel powder, corn cob and cottonseed hulls after absorbing water in the formulated amount are uniformly mixed, the water content is adjusted to 60%, and sterilized at 122° C. for 40 minutes to obtain the Pleurotus geesteranus cultivation medium.
[0105] A method for cultivating Pleurotus geesteranus using the Pleurotus geesteranus cultivation substrate specifically comprises the following steps:
[0106] Preparation of mushroom bags: bag the prepared Pleurotus geesteranus cultivation substrate in a bagging amount of 450 g / bag, sterilize at 122°C for 40 min, and obtain sterilized mushroom bags.
[0107] Inoculation: Inoculate the sterilized bags with an inoculation amount of 20 mg / g. The specific operation method is: move the sterilized bags into the sterilized inoculation room for natural cooling, and inoculate when the bag temperature is 25°C. Before inoculation, put the inoculation tools, rings, plastic covers, and alcohol cotton balls into the inoculation room and fumigate with sodium dichloroisocyanurate, 10g per cubic meter. During inoculation, the operator wipes and disinfects the hands and the surface of the cultivated species with cotton balls. Two people operate, one is responsible for uncovering and covering the cover, and the other is responsible for inoculation. One end is inoculated, and the cultivated species connected seals the surface of the material to obtain an inoculated bag.
[0108] Management of mushroom growth: The temperature is controlled at 25℃, and the contaminated mushroom bags are removed in time. When the mycelium has eaten half of the material, turn the bag over once. When the mycelium is fully grown and there is a hollow drum sound when knocking, the mushroom stick is ready.
[0109] Management of mushroom production: Cultivate the inoculated mushroom bags, and move them into the mushroom shed for mushroom production after the mycelium is fully grown. First, open the air outlet membrane of the mushroom shed, lower the ambient temperature of the mushroom shed to 15℃, stimulate at low temperature for 14 hours, and spray humidification into the space regularly to keep the relative humidity at 95% to stimulate the mycelium to form primordia. After most of the primordia appear, the shed can be sealed for mushroom production. At the same time, the mushroom cap and the collar can be removed. Water cannot be sprayed. Ventilate 10 times a day, 15 minutes each time, control the relative humidity of the air at 95%, and provide scattered light to make the mycelium produce mushrooms as soon as possible under the stimulation of low temperature and light.
[0110] Harvesting: When the fruiting bodies of Pleurotus geesteranus grow to 60% to 70%, that is, the cap grows to 3cm, the edge of the cap curls inward, and the color gradually changes from dark to light, start harvesting before the spores are ejected.
[0111] Comparative Example 1 (using pesticides for insecticide without adding botanical insecticide)
[0112] A cultivation medium for Pleurotus geesteranus consists of the following raw materials in parts by weight: 80 parts of Caragana korshinskii, 40 parts of cottonseed hulls, 5 parts of lime powder and 15 parts of corn cobs.
[0113] A method for preparing a cultivation substrate for Pleurotus geesteranus, comprising the following steps:
[0114] The formulated amount of Caragana korshinskii was crushed into 2 mm to obtain Caragana korshinskii powder.
[0115] The corn cobs in the formula amount are crushed into 5 mm to obtain corn cobs.
[0116] Soak a formulated amount of cottonseed hulls in water until the cottonseed hulls no longer absorb water, thereby obtaining the water-absorbed cottonseed hulls.
[0117] The lime powder, the prepared caragana powder, corn cobs and the cottonseed hulls after absorbing water in the prescribed amount are mixed evenly, the water content is adjusted to 60%, and the mixture is sterilized at 122° C. for 40 minutes to obtain the Pleurotus geesteranus cultivation medium.
[0118] A method for cultivating Pleurotus geesteranus using the Pleurotus geesteranus cultivation substrate specifically comprises the following steps:
[0119] Preparation of mushroom bags: bag the prepared Pleurotus geesteranus cultivation substrate in a bagging amount of 450 g / bag, sterilize at 122°C for 40 min, and obtain sterilized mushroom bags.
[0120] Inoculation: Inoculate the sterilized bags with an inoculation amount of 20 mg / g. The specific operation method is: move the sterilized bags into the sterilized inoculation room for natural cooling, and inoculate when the bag temperature is 25°C. Before inoculation, put the inoculation tools, rings, plastic covers, and alcohol cotton balls into the inoculation room and fumigate with sodium dichloroisocyanurate, 10g per cubic meter. During inoculation, the operator wipes and disinfects the hands and the surface of the cultivated species with cotton balls. Two people operate, one is responsible for uncovering and covering the cover, and the other is responsible for inoculation. One end is inoculated, and the cultivated species connected seals the surface of the material to obtain an inoculated bag.
[0121] Management of mushroom growth: The temperature is controlled at 28℃, and the contaminated mushroom bags are removed in time. When the mycelium has eaten half of the material, turn the bag over once. When the mycelium is fully grown and there is a hollow drum sound when knocking, the mushroom stick is ready.
[0122] Management of mushroom production: Cultivate the inoculated mushroom bags, and move them into the mushroom shed for mushroom production after the mycelium is fully grown. First, open the air outlet membrane of the mushroom shed, lower the ambient temperature of the mushroom shed to 15℃, stimulate at low temperature for 14 hours, and spray humidification into the space regularly to keep the relative humidity at 85% to stimulate the mycelium to form primordia. After most of the primordia appear, the shed can be sealed for mushroom production. At the same time, the mushroom cap and the collar can be removed. Water cannot be sprayed. Ventilate 10 times a day, 15 minutes each time, control the relative humidity of the air at 80%, and provide scattered light to make the mycelium produce mushrooms as soon as possible under the stimulation of low temperature and light.
[0123] Spray Mushroom Insect Net to prevent insect pests. Dilute 4.3% Mushroom Insect Net 1000 times for spraying.
[0124] Harvesting: When the fruiting bodies of Pleurotus geesteranus grow to 60% to 70%, that is, the cap grows to 2cm, the edge of the cap curls inward, and the color gradually changes from dark to light, start harvesting before the spores are ejected.
[0125] Comparative Example 2 (without fennel and orange peel)
[0126] A cultivation medium for Pleurotus geesteranus consists of the following raw materials in parts by weight: 80 parts of Caragana korshinskii, 40 parts of cottonseed hulls, 5 parts of lime powder, 12 parts of celastrus angulatus rhizomes, and 15 parts of corn cobs.
[0127] A method for preparing a cultivation substrate for Pleurotus geesteranus, comprising the following steps:
[0128] The formulated amount of Caragana korshinskii was crushed into 2 mm to obtain Caragana korshinskii powder.
[0129] The corn cobs in the formula amount are crushed into 5 mm to obtain corn cobs.
[0130] The formulated amount of Celastrus angulatus rhizome is crushed into 2 mm to obtain Celastrus angulatus rhizome powder.
[0131] Soak a formulated amount of cottonseed hulls in water until the cottonseed hulls no longer absorb water, thereby obtaining the water-absorbed cottonseed hulls.
[0132] The lime powder, prepared Caragana korshinskii powder, Celastrus angulatus rhizome powder, corn cob pieces and cottonseed hulls after absorbing water in the formulated amount are uniformly mixed, the water content is adjusted to 60%, and sterilized at 122° C. for 40 minutes to obtain the Pleurotus geesteranus cultivation medium.
[0133] A method for cultivating Pleurotus geesteranus using the Pleurotus geesteranus cultivation substrate specifically comprises the following steps:
[0134] Preparation of mushroom bags: bag the prepared Pleurotus geesteranus cultivation substrate in a bagging amount of 450 g / bag, sterilize at 122°C for 40 min, and obtain sterilized mushroom bags.
[0135] Inoculation: Inoculate the sterilized bags with an inoculation amount of 20 mg / g. The specific operation method is: move the sterilized bags into the sterilized inoculation room for natural cooling, and inoculate when the bag temperature is 25°C. Before inoculation, put the inoculation tools, rings, plastic covers, and alcohol cotton balls into the inoculation room and fumigate with sodium dichloroisocyanurate, 10g per cubic meter. During inoculation, the operator wipes and disinfects the hands and the surface of the cultivated species with cotton balls. Two people operate, one is responsible for uncovering and covering the cover, and the other is responsible for inoculation. One end is inoculated, and the cultivated species connected seals the surface of the material to obtain an inoculated bag.
[0136] Management of mushroom growth: The temperature is controlled at 28℃, and the contaminated mushroom bags are removed in time. When the mycelium has eaten half of the material, turn the bag over once. When the mycelium is fully grown and there is a hollow drum sound when knocking, the mushroom stick is ready.
[0137] Management of mushroom production: Cultivate the inoculated mushroom bags, and move them into the mushroom shed for mushroom production after the mycelium is fully grown. First, open the air outlet membrane of the mushroom shed, lower the ambient temperature of the mushroom shed to 15℃, stimulate at low temperature for 14 hours, and spray humidification into the space regularly to keep the relative humidity at 85% to stimulate the mycelium to form primordia. After most of the primordia appear, the shed can be sealed for mushroom production. At the same time, the mushroom cap and the collar can be removed. Water cannot be sprayed. Ventilate 10 times a day, 15 minutes each time, control the relative humidity of the air at 80%, and provide scattered light to make the mycelium produce mushrooms as soon as possible under the stimulation of low temperature and light.
[0138] Harvesting: When the fruiting bodies of Pleurotus geesteranus grow to 60% to 70%, that is, the cap grows to 2cm, the edge of the cap curls inward, and the color gradually changes from dark to light, start harvesting before the spores are ejected.
[0139] Comparative Example 3 (without orange peel)
[0140] A cultivation medium for Pleurotus geesteranus consists of the following raw materials in parts by weight: 80 parts of Caragana korshinskii, 40 parts of cottonseed hulls, 5 parts of lime powder, 12 parts of smilax glabra rhizomes, 9 parts of fennel and 15 parts of corn cobs.
[0141] A method for preparing a cultivation substrate for Pleurotus geesteranus, comprising the following steps:
[0142] The formulated amount of Caragana korshinskii was crushed into 2 mm to obtain Caragana korshinskii powder.
[0143] The corn cobs in the formula amount are crushed into 5 mm to obtain corn cobs.
[0144] The formulated amount of Celastrus angulatus rhizome is crushed into 2 mm to obtain Celastrus angulatus rhizome powder.
[0145] Grind the formulated amount of fennel into 2 mm to obtain fennel powder.
[0146] Soak a formulated amount of cottonseed hulls in water until the cottonseed hulls no longer absorb water, thereby obtaining the water-absorbed cottonseed hulls.
[0147] The lime powder, prepared Caragana korshinskii powder, Caulis angelicae rhizome powder, fennel powder, corn cob pieces and cottonseed hulls after absorbing water in the prescribed amount are uniformly mixed, the water content is adjusted to 60%, and sterilized at 122° C. for 40 minutes to obtain the Pleurotus geesteranus cultivation medium.
[0148] A method for cultivating Pleurotus geesteranus using the Pleurotus geesteranus cultivation substrate specifically comprises the following steps:
[0149] Preparation of mushroom bags: bag the prepared Pleurotus geesteranus cultivation substrate in a bagging amount of 450 g / bag, sterilize at 122°C for 40 min, and obtain sterilized mushroom bags.
[0150] Inoculation: Inoculate the sterilized bags with an inoculation amount of 20 mg / g. The specific operation method is: move the sterilized bags into the sterilized inoculation room for natural cooling, and inoculate when the bag temperature is 25°C. Before inoculation, put the inoculation tools, rings, plastic covers, and alcohol cotton balls into the inoculation room and fumigate with sodium dichloroisocyanurate, 10g per cubic meter. During inoculation, the operator wipes and disinfects the hands and the surface of the cultivated species with cotton balls. Two people operate, one is responsible for uncovering and covering the cover, and the other is responsible for inoculation. One end is inoculated, and the cultivated species connected seals the surface of the material to obtain an inoculated bag.
[0151] Management of mushroom growth: The temperature is controlled at 28℃, and the contaminated bags are removed in time. When the mycelium has eaten half of the material, turn the bag over once. When the mycelium is fully grown and there is a hollow drum sound when knocking, it can be stacked. Generally, the mycelium will fill the bag about 25 days after inoculation, and the mushroom stick production is completed at this time.
[0152] Management of mushroom production: Cultivate the inoculated mushroom bags, and move them into the mushroom shed for mushroom production after the mycelium is fully grown. First, open the air outlet membrane of the mushroom shed, lower the ambient temperature of the mushroom shed to 15℃, stimulate at low temperature for 14 hours, and spray humidification into the space regularly to keep the relative humidity at 85% to stimulate the mycelium to form primordia. After most of the primordia appear, the shed can be sealed for mushroom production. At the same time, the mushroom cap and the collar can be removed. Water cannot be sprayed. Ventilate 10 times a day, 15 minutes each time, control the relative humidity of the air at 80%, and provide scattered light to make the mycelium produce mushrooms as soon as possible under the stimulation of low temperature and light.
[0153] Harvesting: When the fruiting bodies of Pleurotus geesteranus grow to 60% to 70%, that is, the cap grows to 2cm, the edge of the cap curls inward, and the color gradually changes from dark to light, start harvesting before the spores are ejected.
[0154] Comparative Example 4 (without adding Celastrus angulatus)
[0155] A cultivation medium for Pleurotus geesteranus consists of the following raw materials in parts by weight: 80 parts of Caragana korshinskii, 40 parts of cottonseed hulls, 5 parts of lime powder, 9 parts of fennel, 17 parts of orange peels and 15 parts of corn cobs.
[0156] A method for preparing a cultivation substrate for Pleurotus geesteranus, comprising the following steps:
[0157] The formulated amount of Caragana korshinskii was crushed into 2 mm to obtain Caragana korshinskii powder.
[0158] The corn cobs in the formula amount are crushed into 5 mm to obtain corn cobs.
[0159] Grind the formulated amount of fennel into 2 mm to obtain fennel powder.
[0160] The formulated amount of orange peel is crushed into 2 mm to obtain orange peel powder.
[0161] Soak a formulated amount of cottonseed hulls in water until the cottonseed hulls no longer absorb water, thereby obtaining the water-absorbed cottonseed hulls.
[0162] The lime powder, prepared caragana powder, fennel powder, orange peel powder, corn cob and cottonseed hulls after absorbing water in the prescribed amount are mixed evenly, the water content is adjusted to 60%, and sterilized at 122° C. for 40 minutes to obtain the Pleurotus geesteranus cultivation medium.
[0163] A method for cultivating Pleurotus geesteranus using the Pleurotus geesteranus cultivation substrate specifically comprises the following steps:
[0164] Preparation of mushroom bags: bag the prepared Pleurotus geesteranus cultivation substrate in a bagging amount of 450 g / bag, sterilize at 122°C for 40 min, and obtain sterilized mushroom bags.
[0165] Inoculation: Inoculate the sterilized bags with an inoculation amount of 20 mg / g. The specific operation method is: move the sterilized bags into the sterilized inoculation room for natural cooling, and inoculate when the bag temperature is 25°C. Before inoculation, put the inoculation tools, rings, plastic covers, and alcohol cotton balls into the inoculation room and fumigate with sodium dichloroisocyanurate, 10g per cubic meter. During inoculation, the operator wipes and disinfects the hands and the surface of the cultivated species with cotton balls. Two people operate, one is responsible for uncovering and covering the cover, and the other is responsible for inoculation. One end is inoculated, and the cultivated species connected seals the surface of the material to obtain an inoculated bag.
[0166] Management of mushroom growth: The temperature is controlled at 28℃, and the contaminated mushroom bags are removed in time. When the mycelium has eaten half of the material, turn the bag over once. When the mycelium is fully grown and there is a hollow drum sound when knocking, the mushroom stick is ready.
[0167] Management of mushroom production: Cultivate the inoculated mushroom bags, and move them into the mushroom shed for mushroom production after the mycelium is fully grown. First, open the air outlet membrane of the mushroom shed, lower the ambient temperature of the mushroom shed to 15℃, stimulate at low temperature for 14 hours, and spray humidification into the space regularly to keep the relative humidity at 85% to stimulate the mycelium to form primordia. After most of the primordia appear, the shed can be sealed for mushroom production. At the same time, the mushroom cap and the collar can be removed. Water cannot be sprayed. Ventilate 10 times a day, 15 minutes each time, control the relative humidity of the air at 80%, and provide scattered light to make the mycelium produce mushrooms as soon as possible under the stimulation of low temperature and light.
[0168] Harvesting: When the fruiting bodies of Pleurotus geesteranus grow to 60% to 70%, that is, the cap grows to 2cm, the edge of the cap curls inward, and the color gradually changes from dark to light, start harvesting before the spores are ejected.
[0169] Result determination:
[0170] The yield and biological conversion rate of Pleurotus geesteranus cultivated in Pleurotus geesteranus cultivation substrates of different embodiments and comparative examples are shown in Table 1. Only the first 3 flushes of Pleurotus geesteranus were measured. Three replicates were made for each embodiment and comparative example. One-way ANOVA was performed on the data using IBM SPASS 22.0 software.
[0171] Biological efficiency (%) = (yield of mushrooms per batch / weight of dry material per bag) × 100.
[0172] Table 1 Yield and biological conversion rate of Pleurotus geesteranus cultivated in different embodiments and comparative examples
[0173]
[0174] Table 2 Growth of Pleurotus geesteranus fruiting bodies cultivated in different embodiments and comparative examples
[0175]
[0176]
[0177] As shown in Tables 1 and 2, compared with Comparative Examples 1 to 4, the yield, biological conversion rate, flower weight, cap weight and handle weight of Pleurotus geesteranus cultivated in Examples 1 to 5 are all higher. The yield of Comparative Example 1 is slightly lower than that of Examples 1 to 5, which may be because although pesticides are used in Comparative Example 1 to kill insects, the pesticides have certain damage to the fruiting bodies of Pleurotus geesteranus. In the matrix of Comparative Example 2, fennel and orange peel are not added, only Caulis dahliae is added, which has low lethality to pests. Pests cannibalize the fruiting bodies of Pleurotus geesteranus, resulting in a reduction in the yield of Pleurotus geesteranus. In the matrix of Comparative Example 3, orange peel is not added, and orange peel has a strong lethality to springtails. After removing the orange peel, the control ability of springtails may be weakened. In the matrix of Comparative Example 4, Caulis dahliae is not added, and Caulis dahliae has lethality to many pests. Without Caulis dahliae, the control ability of pests is weak.
[0178] Table 3 Nutritional components of Pleurotus geesteranus cultivated in different embodiments and comparative examples
[0179] Protein / (g / 100g) Total sugar / (g / 100g) Reducing sugar / (g / 100g) Example 1 4.50±0.02bc 0.49±0.00e 0.51±0.01b Example 2 4.65±0.01a 0.59±0.00b 0.52±0.02ab Example 3 4.52±0.03b 0.52±0.01d 0.46±0.01c Example 4 4.54±0.06a 0.51±0.002f 0.49±0.02e Example 5 4.58±0.05e 0.55±0.001ab 0.47±0.01f Comparative Example 1 4.46±0.02c 0.58±0.00b 0.54±0.01a Comparative Example 2 4.12±0.03e 0.49±0.01e 0.47±0.00c Comparative Example 3 4.41±0.02d 0.55±0.01c 0.48±0.00c Comparative Example 4 3.80±0.03f 0.61±0.01a 0.52±0.02ab
[0180] As shown in Table 3, the nutritional content of Pleurotus geesteranus cultivated in Comparative Examples 1 and 3 is not much different from that in Examples 1 to 5. The nutritional content of Pleurotus geesteranus cultivated in Comparative Examples 2 and 4 is quite different from that in Examples 1 to 5. In Comparative Example 2, no fennel and orange peel were added, only Celastrus was added, and in Comparative Example 4, Celastrus was not added, indicating that fennel, orange peel, and Celastrus can not only prevent and control pests, but also increase the content of various nutrients in Pleurotus geesteranus.
[0181] Although the preferred embodiments of the present invention have been described, those skilled in the art may make other changes and modifications to these embodiments once they have learned the basic creative concept. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments and all changes and modifications that fall within the scope of the present invention.
[0182] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.
Claims
1. A Pleurotus geesteranus cultivation substrate, characterized in that: The invention is composed of the following raw materials in parts by weight: 60-80 parts of Caragana korshinskii, 20-40 parts of cottonseed hulls, 1-5 parts of lime powder, 6-12 parts of calamus berberis rhizomes, 3-9 parts of fennel, 10-17 parts of orange peels and 15-20 parts of corn cobs.
2. The Pleurotus geesteranus cultivation substrate according to claim 1, characterized in that: The invention is composed of the following raw materials in parts by weight: 65-75 parts of Caragana korshinskii, 25-35 parts of cottonseed hulls, 2-4 parts of lime powder, 8-10 parts of calamus berberis rhizomes, 5-7 parts of fennel, 12-14 parts of orange peels and 16-18 parts of corn cobs.
3. A Pleurotus geesteranus cultivation substrate according to claim 2, characterized in that: The invention is composed of the following raw materials in parts by weight: 70 parts of Caragana korshinskii, 30 parts of cottonseed hulls, 3 parts of lime powder, 9 parts of serrata root stems, 6 parts of fennel, 13 parts of orange peels and 17 parts of corn cobs.
4. The method for preparing a Pleurotus geesteranus cultivation substrate according to claim 1, characterized in that: The specific steps include: Crush the formulated amount of Caragana korshinskii to obtain Caragana korshinskii powder; Grinding corn cobs into pieces of 5 mm to 8 mm to obtain corn cobs; The preparation methods of Caulis Achyranthis Bidentatae rhizome powder, fennel powder and orange peel powder are the same as that of Caragana korshinskii powder; Soaking a formulated amount of cottonseed hulls in water until the cottonseed hulls no longer absorb water, thereby obtaining cottonseed hulls that have absorbed water; The lime powder, prepared Caragana korshinskii powder, Caulis davidiana root and stem powder, fennel powder, orange peel powder, corn cob and cottonseed hulls after absorbing water are mixed evenly, the water content is adjusted to 50% to 60%, and the Pleurotus geesteranus cultivation medium is obtained by sterilization.
5. The method for preparing a cultivation substrate of Pleurotus geesteranus according to claim 1, characterized in that: Crush the caragana to 1mm to 2mm.
6. The method for preparing a Pleurotus geesteranus cultivation substrate according to claim 4, characterized in that: The sterilization condition is 120° C. to 122° C. for 110 min to 120 min.
7. A method for cultivating Pleurotus geesteranus using the Pleurotus geesteranus cultivation substrate according to claim 1, characterized in that: The specific steps include: Making mushroom bags: bagging and sterilizing the prepared Pleurotus geesteranus cultivation substrate to obtain sterilized mushroom bags; Inoculation: Inoculate the sterilized fungus bag to obtain an inoculated fungus bag; Mycelium management: Cultivate the inoculated bacteria bags to obtain bacteria sticks with full mycelium; Fruiting management: Move the mushroom sticks after full mycelium growth into the mushroom shed for fruiting cultivation; Harvesting: Start harvesting when the fruiting bodies of Pleurotus geesteranus grow to 60% to 70%.
8. The method for cultivating Pleurotus geesteranus according to claim 6, characterized in that: During inoculation, the inoculation amount is 10mg / g to 20mg / g.
9. The method for cultivating Pleurotus geesteranus according to claim 7, characterized in that: During mushroom management, the temperature in the mushroom shed is 22℃~25℃ and the relative humidity is 80%~90%.
Citation Information
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