Constant-temperature quantitative sub-packaging super clean table of shaking table and application method thereof

Abstract

The invention relates to the technical field of experimental equipment, in particular to a constant-temperature quantitative split charging super clean table of a shaking table and an application method thereof, the constant-temperature quantitative split charging super clean table comprises a super clean table body, and a constant-temperature box and a conical flask are detachably mounted in the super clean table body; the driving assembly is connected with the constant-temperature box, and the driving assembly can drive the constant-temperature box to reciprocate; the quantitative split charging assembly is arranged in the super clean bench body, and the quantitative split charging assembly is used for quantitatively extracting a mixed solution of a culture medium and a strain in the conical flask; the concentric plate group is arranged in the constant-temperature box, and the concentric plate group can prevent the hot liquid in the constant-temperature box from splashing out; and the suction assembly is provided with a plurality of nozzles and is circumferentially installed in the constant-temperature box at equal intervals, the suction assembly is matched with a guide piece arranged on the driving assembly, and when the constant-temperature box moves to the end of the stroke, the hot liquid in the constant-temperature box can move from the outside of the conical flask to the top down, the heating effect is improved, and the hot liquid is prevented from splashing.

Description

Technical Field

[0001] The invention relates to the technical field of experimental equipment, in particular to a constant-temperature quantitative packaging shaking table clean bench and an application method thereof. Background Art

[0002] A shaker is a biochemical instrument that combines a temperature-controllable constant-temperature heating and insulation tank with an oscillator. It includes two types of shakers: one with gas as the heating medium and the other with water as the heating medium. It is mainly suitable for universities, colleges, medical, petrochemical, health and epidemic prevention, environmental monitoring and other scientific research departments for oscillating and shaking cultures of various liquid and solid compounds such as biology, biochemistry, cells, and bacteria.

[0003] During the use of the shaker, the hot liquid contained in the shaker will oscillate back and forth with the shaker. At this time, due to the effect of inertia, the hot liquid is likely to splash. For this reason, there are requirements for the water capacity of existing water medium shakers. As the water capacity increases, the inertia of the hot liquid also increases, and the splashing phenomenon will become more serious, which seriously limits the water capacity of the shaker. As a result, when heating and keeping warm, part of the test tube, beaker or conical flask is exposed to the heated hot water, resulting in uneven heating of the test tube, beaker or conical flask as a whole, affecting the reproduction of bacteria. Summary of the invention

[0004] The object of the present invention is to provide a constant temperature quantitative packaging shaking table clean bench and an application method thereof, so as to solve the problems raised in the above background technology.

[0005] To achieve the above object, the present invention provides the following technical solutions: A constant temperature quantitative packaging shaking table clean bench, comprising: A clean bench body, wherein a constant temperature box and a conical flask are detachably installed in the clean bench body; A driving assembly connected to the thermostat, the driving assembly can drive the thermostat to reciprocate, so that the hot liquid in the thermostat shakes and the conical flask is evenly heated; A quantitative dispensing component is arranged in the clean bench body, and is used to quantitatively extract the mixed solution of the culture medium and the bacterial strain in the conical flask; A concentric plate group is arranged in the thermostatic box, and the concentric plate group can prevent the hot liquid placed in the thermostatic box from splashing out; The suction assembly is provided with multiple nozzles and is installed in the constant temperature box at equal distances around the circumference. The suction assembly cooperates with a guide member arranged on the driving assembly, and can make the hot liquid in the constant temperature box move from top to bottom from the outside of the conical flask when the constant temperature box moves to the end of the stroke.

[0006] As a further solution of the present invention: the concentric plate group includes multiple groups of annular baffles connected to the inner wall of the constant temperature box, and the widths of the multiple groups of annular baffles increase successively from bottom to top.

[0007] As a further solution of the present invention: the suction assembly includes a first suction cylinder installed on the side of the thermostatic box, and two groups of first one-way valves are arranged on the first suction cylinder, one group of the first one-way valves is connected to the nozzles arranged on the thermostatic box, and the other group of the first one-way valves is connected to the bottom of the thermostatic box; The suction assembly further comprises a first sealing plug sealingly and slidably mounted in the first suction cylinder, a connecting shaft penetrating the first suction cylinder being mounted on the first sealing plug, a first spring being sleeved on the connecting shaft, and a pulling plate being disposed at one end of the connecting shaft away from the first sealing plug; The convex shaft rotatably mounted on the pulling plate is adapted to the guide member.

[0008] As a further solution of the present invention: the guide member is provided with an inclined surface, and the side of the guide member is provided with a first horizontal groove, a vertical groove and a second horizontal groove; A deflecting member is rotatably mounted on one end of the first horizontal slot away from the vertical slot. When the convex shaft moves toward the guiding member, the deflecting member can guide the convex shaft to move along the inclined surface.

[0009] As a further solution of the present invention: the driving assembly includes a guide plate installed in the clean bench body, a sliding member rotatably connected to the constant temperature box is slidably installed on the guide plate, a rack plate is installed on the side of the sliding member, the rack plate is meshed with a gear rotatably installed on the guide plate, and a first motor installed on the guide plate is connected to the gear; The driving assembly also includes a trigger structure connected to the rotating shaft of the thermostat, and the trigger structure can deflect the thermostat by a predetermined angle when the thermostat performs a reciprocating motion.

[0010] As a further solution of the present invention: the trigger structure comprises a plurality of abutment shafts arranged equidistantly on the bottom of the thermostat and a connecting piece detachably connected to the rack plate, a triggering piece is rotatably mounted on the connecting piece, and the connecting piece and the triggering piece are connected by a stopper kit, and the stopper kit can make the triggering piece deflect unidirectionally relative to the connecting piece; The trigger structure also includes an elastic telescopic rod connected to the sliding member, one end of the elastic telescopic rod is rotatably mounted with an abutment wheel, and the abutment wheel is adapted to a limiter connected to the rotating shaft of the thermostat.

[0011] As a further solution of the present invention: the stopper kit includes a second fan-shaped stopper provided on the connecting member and a first fan-shaped stopper provided on the trigger member, the first fan-shaped stopper and the second fan-shaped stopper; A torsion spring is provided at the rotation connection between the trigger member and the connecting member.

[0012] As a further solution of the present invention: a plurality of groups of limiting grooves are equidistantly arranged on the limiting member in a circumferential manner, a protrusion protruding away from the limiting member rotation axis is formed between two adjacent groups of limiting grooves, and the protrusion is connected to the two adjacent limiting grooves via an inclined guiding surface.

[0013] As a further solution of the present invention: the quantitative dispensing assembly includes a suction structure with one end placed in the conical bottle and a threaded driving structure arranged on the clean bench body; The thread drive structure can drive the suction structure to move so as to extract the mixed liquid of the culture medium and the bacterial strain in the conical flask.

[0014] An application method of the shaking clean bench for constant temperature quantitative packaging as described above comprises the following steps: Step 1: Start the clean bench body, then pour the bacteria and culture medium to be cultured into the conical flask, and finally start the drive component; Step 2: The driving assembly drives the thermostat to reciprocate, and under the action of the concentric plate group, the hot liquid in the thermostat is prevented from splashing out; Step 3: When the thermostatic box moves to the end of the stroke, the suction assembly moves under the action of the guide to pump the hot liquid at the bottom of the thermostatic box to the upper part of the conical flask; Step 4: The driving assembly causes the thermostatic box to deflect at a predetermined angle, so that the hot liquid in the thermostatic box is oscillated in a circumferential direction and shaken; Step 5: Control the action of the quantitative dispensing component to pump the mixed solution of the culture medium and the bacterial strain in the conical flask into the culture dish placed in the clean bench body.

[0015] Compared with the prior art, the present invention has the following beneficial effects: By means of the provided suction assembly and guide, when the thermostatic box moves to the end of the stroke, the hot liquid pumped into the first suction cylinder can be sprayed onto the conical flask through the nozzle, and moves from top to bottom along the height direction of the conical flask, so that the hot liquid can evenly exchange heat with the conical flask, ensuring that the entire conical flask is evenly heated. At the same time, since the nozzle is in a state of tilting downward, a water flow tilting downward can be generated at the nozzle, and this part of the water flow can suppress the hot liquid from splashing upward after hitting the conical flask, thereby preventing the hot liquid from splashing outside the conical flask. By setting up the concentric plate group, when the thermostatic box reciprocates and the hot liquid in the thermostatic box moves upward along the inner wall of the thermostatic box, it can achieve step-by-step buffering under the action of multiple groups of annular baffles, and inhibit the hot liquid from moving along the side wall of the thermostatic box. On the one hand, this can reduce the impact degree when the hot liquid hits the annular baffle, and on the other hand, it can effectively buffer the movement of the hot liquid. The combination of the two aspects can further effectively prevent the occurrence of hot liquid splashing. At the same time, when the hot liquid hits the annular baffle, it can produce lateral hot liquid splashing and act on the hot liquid that continues to move upward, and can slow down and buffer the hot liquid moving toward the upper annular baffle. The hot liquid that moves horizontally can also impact the conical flask, so that the conical flask can maintain a good water heating atmosphere. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 The present invention is a structural schematic diagram of an embodiment of a shaking clean bench for constant temperature quantitative packaging.

[0017] Figure 2 This is a schematic diagram of the structure of a shaking clean bench for constant temperature quantitative packaging after the clean bench body is removed in one embodiment.

[0018] Figure 3 This is a schematic diagram of the structure of a shaking clean bench for constant temperature quantitative packaging after the clean bench body and quantitative packaging components are removed in one embodiment.

[0019] Figure 4 A schematic diagram of the internal structure of the first suction cylinder in one embodiment of a shaking clean bench for constant temperature quantitative packaging.

[0020] Figure 5 A schematic diagram of the internal structure of a constant temperature box in one embodiment of a shaking clean bench for constant temperature quantitative packaging.

[0021] Figure 6 A schematic diagram of the structure of a guide member in an embodiment of a shaking clean bench for constant temperature quantitative packaging.

[0022] Figure 7 A schematic diagram of the structure of the abutment shaft in one embodiment of a shaking clean bench for constant temperature quantitative packaging.

[0023] Figure 8 A schematic diagram of the structure of a drive assembly in an embodiment of a shaking clean bench for constant temperature quantitative packaging.

[0024] Fig. 9 A structural explosion diagram of a trigger structure in an embodiment of a shaking clean bench for constant temperature quantitative packaging.

[0025] Fig.10 The present invention is a structural schematic diagram of the cooperation between the trigger member and the abutment shaft in one embodiment of a shaking clean bench for constant temperature quantitative packaging.

[0026] Fig.11 This is an exploded view of the structure of the quantitative filling component in one embodiment of a shaking clean bench for constant temperature quantitative filling.

[0027] In the figure: 1, clean bench body; 2, constant temperature box; 3, conical bottle; 4, annular baffle; 5, nozzle; 6, first suction cylinder; 7, first sealing plug; 8, first spring; 9, connecting shaft; 10, pulling plate; 11, convex shaft; 12, first one-way valve; 13, guide member; 1301, first horizontal groove; 1302, vertical groove; 1303, second horizontal groove; 1304, inclined surface; 14, deflection member; 15, guide plate; 16, sliding member; 17, first motor; 18, gear; 19, gear Slat; 20, limit member; 2001, limit groove; 2002, protrusion; 2003, inclined guide surface; 21, abutment wheel; 22, elastic telescopic rod; 23, abutment shaft; 24, trigger member; 2401, first fan-shaped stop member; 2402, abutment portion; 25, connecting member; 2501, second fan-shaped stop member; 26, second suction cylinder; 27, second one-way valve; 28, second sealing plug; 29, connecting rod; 30, connecting plate; 31, threaded block; 32, second motor; 33, threaded rod. DETAILED DESCRIPTION

[0028] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0029] In addition, when an element in the present invention is referred to as being "fixed to" or "disposed on" another element, it may be directly on the other element or there may be a central element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. The terms "vertical", "horizontal", "left", "right" and similar expressions used herein are for illustrative purposes only and are not intended to be the only embodiment.

[0030] See also Figure 1 to Figure 11In an embodiment of the present invention, a constant temperature quantitative packaging shaking table clean bench includes: a clean bench body 1, a driving component, a quantitative packaging component, a concentric plate group and a suction component, so that when the thermostatic box 2 reciprocates and the hot liquid in the thermostatic box 2 moves upward along the inner wall of the thermostatic box 2, it can achieve step-by-step buffering under the action of multiple groups of annular baffles 4 to inhibit the hot liquid from moving along the side wall of the thermostatic box 2. On the one hand, this can make the impact degree of the hot liquid smaller when it hits the annular baffle 4, and on the other hand, it can effectively buffer the movement of the hot liquid. The combination of the two aspects can effectively prevent the hot liquid from splashing. The occurrence of the situation, at the same time, when the hot liquid hits the annular baffle 4, it can generate lateral hot liquid splashing, and act on the hot liquid that continues to move upward, and can decelerate and buffer the hot liquid moving toward the upper annular baffle 4, and the hot liquid that moves horizontally can also impact on the conical flask 3; wherein, the hot liquid is equivalent to hot water, and a heating component is integrated in the thermostat 2, such as electric heating wire heating, electric heating plate heating, etc., and then cooperates with the temperature control module to achieve constant temperature heating; through the setting of the above structure, the conical flask 3 can maintain a good water heating and heat preservation atmosphere, as follows: The clean bench body 1 is detachably provided with a thermostat 2 and a conical flask 3. Specifically, the upper end of the conical flask 3 is fixed to the clean bench body 1 through an annular clamp. The driving component is connected to the thermostat 2, and the driving component can drive the thermostat 2 to reciprocate, wherein the conical flask 3 contains bacteria and culture medium to be cultured, and the thermostat 2 contains heated hot liquid, and the thermostat 2 has the effect of keeping the heated fluid at a constant temperature. When in use, the driving component can drive the thermostat 2 and the heated hot liquid inside it to reciprocate, so that the hot liquid in the thermostat 2 shakes, and the conical flask 3 is evenly heated; and the heated hot liquid and the conical flask 3 have a better heat exchange effect, ensuring the environmental requirements during bacterial culture. At the same time, the conical flask 3 is in a fixed state, so that the load is lower than that when the conical flask 3 moves synchronously with the thermostat 2, and at the same time, the stability of the bacteria and culture medium to be cultured in the conical flask 3 is improved, and the reproduction effect of the bacteria is improved; The driving assembly includes a guide plate 15 installed in the clean bench body 1, and a sliding member 16 rotatably connected to the constant temperature box 2 is slidably installed on the guide plate 15. A rack plate 19 is installed on the side of the sliding member 16, and the rack plate 19 is meshed with a gear 18 rotatably installed on the guide plate 15. A first motor 17 installed on the guide plate 15 is connected to the gear 18; wherein the above-mentioned first motor 17 is a stepping motor capable of positive and negative rotation. The first motor 17 drives the gear 18 to rotate positive and negative, so that when the gear 18 is meshed with the rack plate 19, the sliding member 16 can be driven to reciprocate along the length direction of the guide plate 15, thereby improving the contact effect between the heated hot liquid in the constant temperature box 2 and the conical flask 3, so that the heated hot liquid can fully contact with the conical flask 3, so as to maintain a suitable bacterial reproduction environment in the conical flask 3.

[0031] The driving assembly further comprises a trigger structure connected to the rotating shaft of the thermostat 2, wherein the trigger structure can deflect the thermostat 2 by a predetermined angle when the thermostat 2 performs a reciprocating motion; The trigger structure includes a plurality of abutment shafts 23 arranged equidistantly on the bottom of the thermostat 2 and a connecting member 25 detachably connected to the rack plate 19, a trigger member 24 is rotatably mounted on the connecting member 25, and the connecting member 25 is connected to the trigger member 24 via a stopper kit, and the stopper kit can make the trigger member 24 deflect unidirectionally relative to the connecting member 25; The trigger structure also includes an elastic telescopic rod 22 connected to the sliding member 16, and an abutment wheel 21 is rotatably installed on one end of the elastic telescopic rod 22. The abutment wheel 21 is adapted to a limiter 20 connected to the rotating shaft of the constant temperature box 2, wherein the length direction of the elastic telescopic rod 22 passes through the rotation center line of the limiter 20, so that when the abutment wheel 21 cooperates with the limiter 20, the force of the limiter 20 can be balanced, and the axial limitation of the constant temperature box 2 can be achieved, thereby ensuring the axial stability of the constant temperature box 2 when the constant temperature box 2 makes reciprocating motion.

[0032] The limiting member 20 is provided with a plurality of groups of limiting grooves 2001 at equal intervals in a circumference, and a protrusion 2002 protruding outward away from the rotation axis of the limiting member 20 is formed between two adjacent groups of limiting grooves 2001, and the protrusion 2002 is connected to the two adjacent limiting grooves 2001 via an inclined guide surface 2003; The stop kit includes a second fan-shaped stopper 2501 arranged on the connecting member 25 and a first fan-shaped stopper 2401 arranged on the trigger member 24. The first fan-shaped stopper 2401 and the second fan-shaped stopper 2501 are further provided with a torsion spring (not shown in the figure) at the rotational connection between the trigger member 24 and the connecting member 25, and an abutment portion 2402 is formed on the trigger member 24.

[0033] In the initial state, the elastic telescopic rod 22 is in a compressed state, and the abutment wheel 21 is in one of the limiting grooves 2001, so that the thermostat 2 can be axially limited, improving the axial stability of the thermostat 2 during reciprocating motion, and under the cooperation of the first motor 17, the gear 18 and the rack plate 19, the sliding member 16 will drive the thermostat 2 to reciprocate, and the connecting member 25 and the trigger member 24 will also reciprocate, and due to the existence of the torsion spring, the first fan-shaped stopper 2401 and the second fan-shaped stopper 2501 are in abutment. The state makes it possible for the thermostat 2 to move laterally for the first time, when the abutting shaft 23 abuts against the abutting portion 2402, since the thermostat 2 is in an axially limited state, the abutting portion 2402 can drive the trigger member 24 to deflect relative to the connecting member 25, and separate the first fan-shaped stopper 2401 from the second fan-shaped stopper 2501, and at the same time the torsion spring is compressed. After the abutting shaft 23 separates from the abutting portion 2402, the trigger member 24 can be reset under the action of the torsion spring, so that the thermostat 2 can maintain an axially stable state when it moves laterally for the first time. When the thermostatic box 2 moves to the end of the stroke, the thermostatic box 2 will move in the reverse direction. At this time, when the abutting portion 2402 abuts against the abutting shaft 23 again, the abutting portion 2402 will not be able to deflect due to the cooperation between the first sector-shaped stopper 2401 and the second sector-shaped stopper 2501, so that the abutting shaft 23 can drive the thermostatic box 2 to rotate, and the abutting wheel 21 and the limiting groove 2001 move, and move along one of the inclined guide surfaces 2003, so that the elastic telescopic rod 22 is compressed and the elastic potential energy is stored until the abutting wheel 21 moves past the protruding portion 200 2, the elastic telescopic rod 22 can actively release the elastic potential energy to make the abutting wheel 21 move along the other inclined guiding surface 2003 toward the other limiting groove 2001, so that the thermostatic box 2 can perform a predetermined angle of deflection, and during the reciprocating motion of the thermostatic box 2, it can perform a linear stepping axial rotation, so that the heated hot liquid can evenly act on the outer circumference of the conical flask 3, thereby improving the heat exchange effect between the heated hot liquid and the conical flask 3, ensuring that the bacteria and the culture medium in the conical flask 3 are evenly heated, and providing a suitable temperature environment for bacteria reproduction.

[0034] Through the above arrangement, the thermostat box 2 can rotate a predetermined angle during a reciprocating motion of the thermostat box 2, so that during the continuous reciprocating motion of the thermostat box 2, it can perform linear stepping axial rotation, so that the heated hot liquid can evenly act on the outer circumference of the conical flask 3, thereby improving the heat exchange effect between the heated hot liquid and the conical flask 3, ensuring that the bacteria and culture medium in the conical flask 3 are evenly heated, and providing a suitable temperature environment for bacterial reproduction.

[0035] See also Figure 2 , Fig.11The quantitative sub-packaging component is arranged in the clean bench body 1, and the quantitative sub-packaging component is used to quantitatively extract the mixed liquid of the culture medium and the bacterial strain in the conical bottle 3; The quantitative dispensing assembly includes a suction structure with one end placed in the conical bottle 3 and a threaded driving structure arranged on the clean bench body 1; The threaded drive structure can drive the suction structure to move so as to extract the mixed liquid of the culture medium and the bacterial strain in the conical flask 3, wherein the extraction structure includes a second suction cylinder 26 installed on the clean bench body 1, and two groups of second one-way valves 27 are arranged on the second suction cylinder 26, wherein one group of the second one-way valves 27 extends to the interior of the conical flask 3 through a conduit, and a second sealing plug 28 is sealingly and slidably installed in the second suction cylinder 26, and a connecting rod 29 that passes through the second suction cylinder 26 is connected to the second sealing plug 28, and the connecting rod 29 is connected to the threaded drive structure; The threaded drive structure includes a second motor 32 installed on the clean bench body 1, a threaded rod 33 is connected to the output shaft of the second motor 32, a threaded block 31 threadedly connected to the threaded rod 33 is provided on the threaded rod 33, and a connecting plate 30 connected to the connecting rod 29 is provided on the threaded block 31.

[0036] After the initial stage of bacterial cultivation is completed, the second motor 32 is controlled to work. At this time, the threaded rod 33 and the threaded block 31 cooperate to drive the connecting plate 30 to move, and the second sealing plug 28 is driven to move through the connecting rod 29, so that negative pressure can be generated in the second suction cylinder 26. Under the action of the negative pressure, one of the second one-way valves 27 is turned on, and the bacteria in the conical flask 3 are drawn into the second suction cylinder 26. After a predetermined dose is extracted, the second motor 32 reverses, and the second sealing plug 28 reverses, and the bacteria entering the second suction cylinder 26 can be squeezed out from the other second one-way valve 27 and dripped onto the culture dish, thereby realizing quantitative packaging of bacteria.

[0037] Furthermore, the second motor 32 is a stepping motor capable of forward and reverse rotation, and due to the threaded arrangement between the threaded rod 33 and the threaded block 31, more precise dosage control can be achieved when extracting bacteria, thereby achieving more precise packaging.

[0038] See also Figure 5 The concentric plate group is arranged in the constant temperature box 2. The concentric plate group can prevent the hot liquid placed in the constant temperature box 2 from splashing out. The concentric plate group includes multiple groups of annular baffles 4 connected to the inner wall of the constant temperature box 2. The width of the multiple groups of annular baffles 4 increases successively from bottom to top.

[0039] It is worth noting that the annular baffle 4 is coaxially arranged with the thermostatic box 2, and multiple groups of annular baffles 4 are equidistantly arranged in the vertical direction of the space. At this time, the widths of the multiple groups of annular baffles 4 increase successively from bottom to top. This makes it possible for the annular baffle 4 to stop the hot liquid in the thermostatic box 2 when it splashes outward along the side wall of the thermostatic box 2 due to inertia when the thermostatic box 2 reciprocates and turns back, so that the thermostatic box 2 can reciprocate with a larger amplitude and a faster speed, thereby ensuring the water heating and heat preservation effect while avoiding hot liquid splashing.

[0040] Specifically, when the hot liquid in the thermostatic box 2 moves upward along the side wall of the thermostatic box 2 due to inertia, it will contact the bottom annular baffle 4. At this time, the bottom annular baffle 4 can stop part of the hot liquid and suppress its movement. Since the bottom annular baffle 4 has the smallest width, some hot liquid will still move upward along the side wall of the thermostatic box 2. At this time, the annular baffle 4 above the annular baffle 4 can stop the hot liquid that is still moving upward along the side wall of the thermostatic box 2, that is, multiple groups of annular baffles 4 can stop the hot liquid step by step and suppress its movement along the side wall of the thermostatic box 2. On the one hand, the impact degree of the hot liquid when it hits the annular baffle 4 is smaller, and on the other hand, the movement of the hot liquid can be effectively buffered. The combination of the two aspects can effectively prevent the occurrence of hot liquid splashing.

[0041] Furthermore, since the annular baffle 4 is arranged horizontally, when the hot liquid contacts and collides with the lowest annular baffle 4, this part of the hot liquid collides with the annular baffle 4 and can produce lateral hot liquid splashing. At this time, the hot liquid splashing can act on the other part of the hot liquid that continues to move toward the upper annular baffle 4, thereby slowing down and buffering the hot liquid moving toward the upper annular baffle 4, and the hot liquid doing lateral horizontal movement can also impact on the conical flask 3, so that the conical flask 3 can maintain a good water heating and heat preservation atmosphere.

[0042] Through the above-mentioned arrangement, when the thermostatic box 2 reciprocates and the hot liquid in the thermostatic box 2 moves upward along the inner wall of the thermostatic box 2, step-by-step buffering can be achieved under the action of multiple groups of annular baffles 4, thereby inhibiting the hot liquid from moving along the side wall of the thermostatic box 2. On the one hand, this can reduce the impact degree of the hot liquid when it hits the annular baffle 4, and on the other hand, it can effectively buffer the movement of the hot liquid. The combination of the two aspects can effectively prevent the occurrence of hot liquid splashing. At the same time, when the hot liquid hits the annular baffle 4, it can produce lateral hot liquid splashing and act on the hot liquid that continues to move upward, and can slow down and buffer the hot liquid moving toward the upper annular baffle 4. The hot liquid that moves horizontally can also impact the conical flask 3, so that the conical flask 3 can maintain a good hot bath atmosphere.

[0043] See also Figure 2~Figure 4 , Figure 6The suction assembly is provided with multiple nozzles and is equidistantly installed in the thermostat 2. The suction assembly cooperates with the guide 13 provided on the driving assembly, and can make the hot liquid in the thermostat 2 move from the outside of the conical flask 3 from top to bottom when the thermostat 2 moves to the end of the stroke; The suction assembly includes a first suction cylinder 6 installed on the side of the thermostatic box 2, and two groups of first one-way valves 12 are arranged on the first suction cylinder 6, one group of the first one-way valves 12 is connected to the nozzle 5 arranged on the thermostatic box 2, and the other group of the first one-way valves 12 is connected to the bottom of the thermostatic box 2; The suction assembly further comprises a first sealing plug 7 sealingly and slidably mounted in the first suction cylinder 6, a connecting shaft 9 penetrating the first suction cylinder 6 is mounted on the first sealing plug 7, a first spring 8 is sleeved on the connecting shaft 9, one end of the first spring 8 is connected to the first sealing plug 7, and the other end is connected to the inner wall of the first suction cylinder 6, and a pulling plate 10 is arranged at the end of the connecting shaft 9 away from the first sealing plug 7; The convex shaft 11 rotatably mounted on the pulling plate 10 is adapted to the guide member 13 , the guide member 13 is provided with an inclined surface 1304 , and the side of the guide member 13 is provided with a first horizontal groove 1301 , a vertical groove 1302 and a second horizontal groove 1303 ; A deflecting member 14 is rotatably mounted on one end of the first horizontal slot 1301 away from the vertical slot 1302 . When the convex shaft 11 moves toward the guiding member 13 , the deflecting member 14 can guide the convex shaft 11 to move along the inclined surface 1304 .

[0044] In the initial state, the thermostatic box 2 is in an axially limited state. At this time, when the thermostatic box 2 moves horizontally to the end of the stroke, the convex shaft 11 can abut against the deflection member 14, and drive the deflection member 14 to deflect to a state coplanar with the inclined surface 1304. At this time, the thermostatic box 2 continues to move, and can make the convex shaft 11 enter the inclined surface 1304 under the guidance of the deflection member 14. In this process, the convex shaft 11 moves along the inclined surface 1304, and pulls the first sealing plug 7 downward through the pulling plate 10 and the connecting shaft 9, compressing the first spring 8 while generating negative pressure in the first suction cylinder 6. At this time, the hot liquid in the thermostatic box 2 can be sucked into the first suction cylinder 6 through one group of first one-way valves 12, and as the thermostatic box 2 continues to move, the convex shaft 11 can enter the second horizontal groove 1303 from the inclined surface 1304, and after the convex shaft 11 moves to the end of the second horizontal groove 1303, the thermostatic box 2 moves to the end of the stroke. At this time, the first spring 8 releases its elastic potential energy, so that the convex shaft 11 moves along the vertical groove 1302, and at the same time drives the first sealing plug 7 to move upward to squeeze the hot liquid in the first suction cylinder 6, so that this part of the hot liquid can enter the nozzle 5 through another group of first one-way valves 12 and be sprayed on the conical flask 3. Using the gravity of the hot liquid itself, the hot liquid can move from top to bottom along the height direction of the conical flask 3, so that the hot liquid can evenly exchange heat with the conical flask 3, ensuring that the entire conical flask 3 is heated evenly.

[0045] When the thermostat 2 moves in the reverse direction, the convex shaft 11 can move along the first horizontal groove 1301 until it abuts against the deflection member 14, thereby deflecting the deflection member 14. Then, after the deflection member 14 is separated from the convex shaft 11, the deflection member 14 can automatically reset under the action of gravity.

[0046] It should be noted that the above-mentioned connecting piece 25 is located in the middle position of the rack plate 19, so that when the rack plate 19 and the thermostatic box 2 move to the middle position, the rotation of the thermostatic box 2 can be completed, so that when the thermostatic box 2 moves to the end of the stroke, the corresponding suction assembly can be activated to spray the heated hot liquid onto the conical flask 3.

[0047] It should also be noted that the portion of the nozzle 5 in the thermostatic box 2 is in a tilted downward state. At this time, after the hot liquid is ejected from the nozzle 5, it can be sprayed obliquely downward on the conical flask 3. Since the hot liquid can produce lateral sputtering when it hits the annular baffle 4 due to inertia, this part of the hot liquid also has the effect of sputtering upward after hitting the conical flask 3. At this time, a water flow tilted downward is generated at the nozzle 5. This part of the water flow can suppress the hot liquid from splashing upward after hitting the conical flask 3, thereby further avoiding the hot liquid from splashing to the outside of the conical flask 3.

[0048] Through the above arrangement, when the thermostatic box 2 moves to the end of the stroke, the hot liquid pumped into the first suction cylinder 6 can be sprayed onto the conical flask 3 through the nozzle 5, and moves from top to bottom along the height direction of the conical flask 3, so that the hot liquid can evenly exchange heat with the conical flask 3, ensuring that the entire conical flask 3 is heated evenly. At the same time, since the nozzle 5 is in a tilted downward state, a tilted downward water flow can be generated at the nozzle 5. This part of the water flow can suppress the hot liquid from splashing upward after hitting the conical flask 3, thereby further avoiding the hot liquid from splashing to the outside of the conical flask 3.

[0049] As an embodiment of the present invention, the present invention also proposes an application method of the shaking clean bench for constant temperature quantitative packaging as described above, comprising the following steps: Step 1: Start the clean bench body 1, then pour the bacteria to be cultured and the culture medium into the conical flask 3, and finally start the drive assembly; Step 2: The driving assembly drives the thermostat 2 to reciprocate, and under the action of the concentric plate group, the hot liquid in the thermostat 2 is prevented from splashing out; Step 3: When the thermostatic box 2 moves to the end of the stroke, the suction assembly moves under the action of the guide 13 to pump the hot liquid at the bottom of the thermostatic box 2 to the upper part of the conical flask 3; Step 4: The driving assembly causes the thermostatic box 2 to deflect at a predetermined angle, so that the hot liquid in the thermostatic box 2 is oscillated and shaken in a circumferential direction; Step 5: Control the action of the quantitative dispensing component to pump the mixed solution of the culture medium and the bacterial strain in the conical flask 3 into the culture dish placed in the clean bench body 1.

[0050] It will be apparent to those skilled in the art that the invention is not limited to the details of the exemplary embodiments described above and that the invention can be implemented in other specific forms without departing from the spirit or essential features of the invention. Therefore, the embodiments should be considered exemplary and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description, and it is intended that all variations falling within the meaning and scope of the equivalent elements of the claims be included in the invention. Any reference numeral in a claim should not be considered as limiting the claim to which it relates.

[0051] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A constant temperature quantitative packaging shaker clean bench, comprising: A clean bench body (1), wherein a constant temperature box (2) and a conical flask (3) are detachably installed in the clean bench body (1); a drive assembly connected to the thermostatic box (2), the drive assembly being capable of driving the thermostatic box (2) to reciprocate so as to cause the hot liquid in the thermostatic box (2) to shake, thereby causing the conical flask (3) to be heated evenly; A quantitative dispensing component is arranged in the clean bench body (1), and is used to quantitatively extract a mixed solution of the culture medium and the bacterial strain in the conical flask (3); It is characterized by further comprising: A concentric plate group, arranged in the thermostatic box (2), the concentric plate group being capable of preventing hot liquid placed in the thermostatic box (2) from splashing out; A suction assembly is provided with multiple nozzles and is equidistantly installed in a constant temperature box (2). The suction assembly cooperates with a guide (13) provided on the drive assembly to enable the hot liquid in the constant temperature box (2) to move from top to bottom from the outside of the conical flask (3) when the constant temperature box (2) moves to the end of the stroke.

2. A constant temperature quantitative packaging shaking table clean bench according to claim 1, characterized in that: The concentric plate group comprises a plurality of groups of annular baffles (4) connected to the inner wall of the thermostatic box (2), and the widths of the plurality of groups of annular baffles (4) increase sequentially from bottom to top.

3. A constant temperature quantitative packaging shaking table clean bench according to claim 1, characterized in that: The suction assembly comprises a first suction cylinder (6) mounted on the side of the thermostatic box (2), and two groups of first one-way valves (12) are arranged on the first suction cylinder (6), one group of the first one-way valves (12) is connected to a nozzle (5) arranged on the thermostatic box (2), and the other group of the first one-way valves (12) is connected to the bottom of the thermostatic box (2); The suction assembly further comprises a first sealing plug (7) sealingly and slidably mounted in the first suction cylinder (6); a connecting shaft (9) penetrating the first suction cylinder (6) is mounted on the first sealing plug (7); a first spring (8) is sleeved on the connecting shaft (9); and a pulling plate (10) is arranged at one end of the connecting shaft (9) away from the first sealing plug (7); A convex shaft (11) rotatably mounted on the pulling plate (10) is adapted to fit the guide member (13).

4. A constant temperature quantitative packaging shaking table clean bench according to claim 3, characterized in that: The guide member (13) is provided with an inclined surface (1304), and the side of the guide member (13) is provided with a first horizontal groove (1301), a vertical groove (1302) and a second horizontal groove (1303); A deflection member (14) is rotatably mounted on one end of the first horizontal groove (1301) away from the vertical groove (1302); when the convex shaft (11) moves toward the guide member (13), the deflection member (14) can guide the convex shaft (11) to move along the inclined surface (1304).

5. A constant temperature quantitative packaging shaking clean bench according to claim 1, characterized in that: The driving assembly comprises a guide plate (15) mounted in the clean bench body (1), a sliding member (16) rotatably mounted on the guide plate (15) and connected to the constant temperature box (2), a rack plate (19) being mounted on the side of the sliding member (16), the rack plate (19) being meshed with a gear (18) rotatably mounted on the guide plate (15), and a first motor (17) mounted on the guide plate (15) being connected to the gear (18); The driving assembly further comprises a trigger structure connected to the rotating shaft of the thermostatic box (2), wherein the trigger structure is capable of causing the thermostatic box (2) to deflect by a predetermined angle when the thermostatic box (2) performs a reciprocating motion.

6. A constant temperature quantitative packaging shaking table clean bench according to claim 5, characterized in that: The trigger structure comprises a plurality of abutment shafts (23) arranged equidistantly on a circumference at the bottom of the thermostatic box (2) and a connecting piece (25) detachably connected to the rack plate (19); a trigger piece (24) is rotatably mounted on the connecting piece (25); and the connecting piece (25) and the trigger piece (24) are connected via a stopper kit, wherein the stopper kit can cause the trigger piece (24) to deflect unidirectionally relative to the connecting piece (25); The trigger structure further comprises an elastic telescopic rod (22) connected to the sliding member (16), an abutment wheel (21) being rotatably mounted on one end of the elastic telescopic rod (22), the abutment wheel (21) being adapted to a limiter (20) connected to a rotating shaft of the thermostatic box (2).

7. A constant temperature quantitative packaging shaking table clean bench according to claim 6, characterized in that: The stop kit comprises a second fan-shaped stopper (2501) arranged on the connecting member (25) and a first fan-shaped stopper (2401) arranged on the trigger member (24), wherein the first fan-shaped stopper (2401) and the second fan-shaped stopper (2501); A torsion spring is provided at the rotation connection between the trigger member (24) and the connecting member (25).

8. A constant temperature quantitative packaging shaking table clean bench according to claim 6, characterized in that: The limiting member (20) is provided with a plurality of groups of limiting grooves (2001) at equal intervals in a circumferential manner, a protrusion (2002) protruding outwardly away from the rotation axis of the limiting member (20) is formed between two adjacent groups of limiting grooves (2001), and the protrusion (2002) is connected to two adjacent limiting grooves (2001) via an inclined guide surface (2003).

9. A constant temperature quantitative packaging shaking table clean bench according to claim 1, characterized in that: The quantitative dispensing assembly comprises a suction structure with one end placed in the conical flask (3) and a threaded drive structure arranged on the clean bench body (1); The threaded drive structure can drive the suction structure to move, so as to extract the mixed liquid of the culture medium and the bacterial strain in the conical flask (3).

10. An application method of the constant temperature quantitative packaging shaking table clean bench according to any one of claims 1 to 9, characterized in that: The following steps are involved: Step 1: Start the clean bench body (1), then pour the bacteria to be cultured and the culture medium into the conical flask (3), and finally start the drive component; Step 2: The driving component drives the thermostatic box (2) to reciprocate, and under the action of the concentric plate group, prevents the hot liquid in the thermostatic box (2) from splashing out; Step 3: When the thermostatic box (2) moves to the end of the stroke, the suction assembly is activated under the action of the guide (13) to pump the hot liquid at the bottom of the thermostatic box (2) to the upper part of the conical flask (3); Step 4: the driving component causes the thermostatic box (2) to deflect at a predetermined angle, so that the hot liquid in the thermostatic box (2) is oscillated in a circumferential direction and shaken; Step 5: Control the action of the quantitative dispensing component to pump the mixed solution of the culture medium and the bacterial strain in the conical flask (3) into the culture dish placed in the clean bench body (1).

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