Natural plant extract face cream and preparation method thereof

Abstract

The invention discloses natural plant extract face cream and a preparation method thereof. The polysaccharide content is taken as an index, and the extraction process of the cortex dictamni is optimized by adopting a single-factor response surface method; by taking the ABTS free radical scavenging rate as an index, screening the dosages of the broken tremella fuciformis extracting solution, the cortex dictamni extracting solution, the bletilla striata extracting solution and the ginseng extracting solution, and determining the proportion of each extracting solution; sensory evaluation comprehensive scores are taken as indexes, dosage screening is carried out on auxiliary materials including glyceryl stearate, cetostearyl alcohol, stearic acid, coconut oil, glycerin and 1, 2-propylene glycol by utilizing a single factor experiment, a response surface design is carried out by selecting the proportion of key auxiliary materials to a mixed extracting solution, and quality evaluation, functional evaluation and safety evaluation are carried out on the face cream. The ideal natural plant face cream is obtained. The face cream is mild in formula, and has the effects of antioxidant activity, whitening, moisture retention and the like.

Description

Technical Field

[0001] The invention relates to the technical field of natural plant skin care products, and in particular to a facial cream formula using a natural plant extract as an active ingredient and a preparation method thereof. Background Art

[0002] Based on the theory of traditional Chinese medicine, ingredients derived from nature are used as the core elements of skin care products to develop multifunctional, safe and healthy skin care products. This is gradually becoming the mainstream trend of the market, and it is expected that they will dominate the field of skin care products in the future.

[0003] The Dictamni cortex added by the present invention is a traditional Chinese medicinal material. Modern research generally finds that it contains multiple biological activities, such as antibacterial, anti-inflammatory, and antioxidant. By optimizing the extraction process, the extraction rate of the effective ingredients in the Dictamni cortex can be improved, providing economical and high-quality raw materials for the subsequent research and development of facial creams, thereby improving the quality, stability, and skin care effects of facial creams; Bletilla striata is a traditional Chinese medicine in the field of wound repair. In modern research, Bletilla striata can stop bleeding, fight inflammation and inhibit bacteria, resist oxidation, and regulate immunity. At the cellular level, Bletilla striata can promote cell proliferation and microvascular regeneration, and at the tissue level, it can effectively inhibit scar hyperplasia, providing comprehensive support for wound repair. With the continuous deepening and expansion of skin care scientific research, due to the multiple advantages of ginseng polysaccharides, it has become an important ingredient in skin care products. It is added to various skin care products such as facial masks, facial creams, and essences. The main active ingredient is Tremella polysaccharide, which has multiple activities such as anti-oxidation, lowering blood lipids, protecting and nourishing nerves, regulating immunity, inflammation, anti-tumor, and anti-aging. Cao Ming et al. found that Tremella polysaccharide is a natural heteropolysaccharide with excellent moisturizing and film-forming effects. Experiments also showed that Tremella polysaccharide can inhibit the expression of inflammatory factors, protect and restore the barrier function of the skin. Tremella will inevitably produce broken Tremella during processing. In order to reduce losses and save costs, the present invention intends to develop broken Tremella, providing a basis for fully recycling resources.

[0004] Coconut oil, as the oil phase, is an excellent natural moisturizer that can effectively lock in skin moisture, keep the skin soft and smooth, and is rich in fatty acids, which can help repair damaged skin barriers and promote the skin's self-repair ability; glycerin and 1,2-propylene glycol, as the water phase, have the effects of high-efficiency moisturizing and softening keratin; allantoin is a natural moisturizing factor synthesized in the body, which has the effects of moisturizing and softening keratin, and the solubility of allantoin can make skin care products easier to distribute evenly on the skin, improve the skin's ability to absorb other effective ingredients, thereby enhancing the efficacy of skin care products; D-panthenol has good moisturizing and emollient properties, and is often used in skin care products, cosmetics and various personal care products. It can help improve the skin's moisturizing ability, promote wound healing, and increase the skin's softness and smoothness; tocotrienol has antioxidant, anti-inflammatory, moisturizing and other effects, triethanolamine is used to adjust pH, and phenoxyethanol is used as a preservative.

[0005] The invention adopts a hot water reflux method to optimize the extraction process of the Dictamni cortex, and uses the polysaccharide content as an inspection index for the optimization process. The Dictamni cortex water extract is compounded with the broken Tremella fuciformis, Bletilla striata, and Ginseng water extract as main components, and glycerol stearate, cetearyl alcohol, and stearic acid are selected as emulsifiers, which have the functions of stabilizer, emulsifier, thickener, etc.; through the research on the functionalities of the cream such as the antioxidant activity, whitening property, and moisturizing property, a human skin safety irritation evaluation method is adopted to provide a strong guarantee for the safety and reliability of the cream. Summary of the invention

[0006] The purpose of the present invention is to obtain a natural, safe, mild, non-irritating, easily absorbed facial cream with the functions of anti-oxidation, whitening, moisturizing and locking water, balancing skin water and oil, etc. It is suitable for people with sensitive skin, improves the application value of natural plant extracts, and promotes the development of the market cosmetics industry in the direction of natural, environmentally friendly and efficient.

[0007] The invention discloses a natural plant facial cream with the main raw material of Dictamni cortex and having the effects of anti-oxidation, whitening, moisturizing and locking moisture, and balancing water and oil in the skin. The cream combines four kinds of natural plant water extracts, glyceryl stearate, cetearyl alcohol, stearic acid, coconut oil, glycerin, 1,2-propylene glycol, D-panthenol, allantoin, tocotrienol, triethanolamine, and phenoxyethanol as auxiliary materials, and performs functional evaluation and safety evaluation on the facial cream.

[0008] The technical solution adopted by the present invention to solve its technical problem is:

[0009] The invention discloses a natural plant facial cream which takes broken tremella, white daikon bark, bletilla striata and ginseng as main raw materials and has the functions of anti-oxidation, whitening, moisturizing and locking moisture, and balancing water and oil in the skin.

[0010] The broken tremella extract is prepared as follows: 20 g of broken tremella powder is weighed and mixed with 800 ml of pure water, and added into a distillation flask, and extracted under hot reflux at 80° C. for 3.5 h, cooled, and filtered to obtain a tremella polysaccharide extract with a sugar content of 7.2%.

[0011] Preferably, the Dictamni cortex water extract is prepared by the following method: 20 g of Dictamni cortex is crushed, the solid-liquid ratio is 30:1 (mL / g), heated and extracted twice at 81° C., the extraction time is 2 h, filtered through 3 layers of gauze, centrifuged at 4800 r / min for 15 min, and the supernatant is taken, and the polysaccharide content is 44.74%.

[0012] Preferably, the bletilla striata water extract is prepared according to the following method: weigh 20g of bletilla striata powder, add 200ml of water, soak for half an hour, extract for 1.5h, cool and filter; add 120ml of pure water to the residue, extract for 1h, cool and filter; add 120ml of water to the residue, extract for 0.5h, cool and filter, combine the three filtrates to obtain a bletilla striata extract with a polysaccharide content of 30.3%.

[0013] Preferably, the ginseng water extract is prepared as follows: weigh 20 g of ginseng powder and mix with 600 ml of pure water, add them into a distillation flask, extract at 60° C. for 2 h, cool and filter to obtain a ginseng extract with a polysaccharide content of 37.9%.

[0014] Preferably, the facial cream extract is prepared by using high-quality Tremella and broken Tremella to prepare a mixed extract, and the antioxidant activity and tyrosinase inhibition rate are functionally evaluated. The IC of the mixed high-quality Tremella to scaveng DPPH and ABTS free radicals is 50 The values ​​were 1.88 mg / mL and 1.29 mg / mL respectively, and the tyrosinase inhibition rate was 79.63%; the IC values ​​of the mixed broken Tremella extract for scavenging DPPH and ABTS free radicals were 50 The values ​​were 1.94 mg / mL and 1.40 mg / mL respectively, the tyrosinase inhibition rate was 74.27%, and the antioxidant and whitening effects of broken Tremella fuciformis tended to decrease, but there was no statistical difference. It can be used as a high-quality raw material to achieve the purpose of resource recycling.

[0015] Preferably, calculated by volume, the ratios of the broken Tremella fuciformis, Dictamni cortex, Bletilla striata and ginseng are 2.25:2:2:1.5 respectively, and calculated by weight percentage, the mixed ratio of the extract accounts for 4% of the weight of the cream.

[0016] Preferably, calculated by weight, 3-7% glyceryl stearate, 0.5-2.5% cetearyl alcohol, 2-6% stearic acid, 16-24% coconut oil, 6-10% glycerol, 0.5-2.5% 1,2-propylene glycol, 2-6% mixed extract, 57.5-66.5% ultrapure water, 0.5% D-panthenol, 0.5% allantoin, 0.5% tocotrienol, 0.5% triethanolamine and 0.2% phenoxyethanol.

[0017] Preferably, the preparation method of the present formula adopts defoaming treatment, and the obtained facial cream is more uniform and stable.

[0018] Preferably, the natural plant cream is functionally evaluated, and the 12h moisturizing rate of the cream is 48.58%, the 24h moisturizing rate is 41.88%, and the moisturizing effect of the cream is good; the skin water and oil determination score is between 60-85 points, and the effect of balancing the skin water and oil is good.

[0019] Compared with the prior art, the present invention has the following significant advantages:

[0020] 1. The present invention adopts a hot water immersion reflux method to optimize the extraction process of the main material Dictamni cortex, which can retain the antibacterial active ingredients in the plant to the maximum extent and provide economical and high-quality raw materials.

[0021] By using antioxidant screening and matching of four kinds of extracts, including broken Tremella fuciformis, Dictamni cortex, Bletilla striata and Ginseng, the multiple functions of the raw materials in cosmetics can be brought into play.

[0022] 2. The present invention compares the antioxidant and whitening functions of the mixed extracts of high-quality Tremella and broken Tremella, respectively, and confirms that the mixed extracts of broken Tremella have the same high-quality antioxidant and whitening effects, thereby achieving the purpose of cost saving and resource recycling, and increasing the added value of Tremella.

[0023] 3. The present invention determines the dosage of the mixed extract and auxiliary materials through single factor and response surface methodology, optimizes the formula, and improves the stability and uniformity of the cream through specific emulsification and defoaming treatment to ensure product quality.

[0024] 4. The plant facial cream of the present invention has multiple functions such as anti-oxidation, whitening and moisturizing, and is safe and non-irritating. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 Comparison of antioxidant activity of Vc and extracts ABTS (left) and DPPH (right).

[0026] Figure 2 Three-dimensional response surface diagram of the interaction effects of various factors on polysaccharide extraction rate.

[0027] Figure 3Effect of the amount of added broken Tremella fuciformis, Dictamni cortex, Bletilla striata and Ginseng on the clearance rate.

[0028] Figure 4 Effect of extracts on the removal rate and odor of facial creams.

[0029] Figure 5 The influence of the mass fraction of each excipient on the comprehensive score of the cream.

[0030] Figure 6 Three-dimensional response surface diagram of the interaction of various factors on the comprehensive score of face cream.

[0031] Figure 7 Flow chart of the facial cream preparation process.

[0032] Figure 8 Moisturizing rate comparison chart. DETAILED DESCRIPTION

[0033] Example 1: Determination of polysaccharide content

[0034] Drawing of glucose standard curve: Accurately pipette 1mL of standard solution, then quickly add 6mL of phenol-sulfuric acid solution, shake, and let stand for 30 minutes. Measure the absorbance value at a wavelength of 490nm. Draw the standard curve with glucose concentration (C) as the horizontal axis and absorbance value (A) as the vertical axis, and obtain the regression equation and calculate the correlation coefficient (R 2 )

[0035] The standard curve is y = 9.185x + 0.0643, R 2 =0.9997.

[0036] Example 2: Evaluation of the functionality of the extract

[0037] Broken Tremella usually refers to some wastes generated in the Tremella processing process, including Tremella pedicles, fungus residue waste, and some Tremella fragments or powders generated in the process of cleaning, cutting, drying, grinding and crushing Tremella. Although these broken Tremella are regarded as wastes in the traditional processing process, their utilization value is still to be developed. The present invention respectively conducts antioxidant activity and tyrosinase inhibition experiments on the mixed plant extracts added with high-quality Tremella and broken Tremella extracts.

[0038] (1) Antioxidant activity test

[0039] ABTS free radical scavenging experiment: add 2.0 ml ABTS solution to 2.0 ml plant extract, shake for 10 seconds, stand in the dark for 6 minutes, and measure the absorbance A1 at 734 nm. Use VC with the same concentration gradient to replace the Dictamni extract solution as a positive control, and calculate the scavenging rate (%):

[0040] Clearance rate (%) = [(A0-A1) / A0] × 100%

[0041] Where A1 is the absorbance of the active product, and A0 is the absorbance of the blank control sample (no active product).

[0042] IC of Vc 50 The IC value of ABTS free radical scavenging of mixed and crushed plant extracts is 0.003 mg / mL. 50 The values ​​were 1.29mg / mL and 1.4mg / mL respectively. The results showed that it had good antioxidant effect within 0.4-2mg / ml. Figure 1 (Left) shown.

[0043] DPPH free radical scavenging experiment: Mix 2mL ethanol solution of 0.1mmol / LDPPH with 2mL aqueous solution of plant extract and shake well, react in the dark for 30min, measure the absorbance at 517nm, and get A1. Ascorbic acid (VC) is used as a positive control, and a mixed solution of 2mL DPPH solution plus 2mL anhydrous ethanol is used as a blank control, and the absorbance at 517nm is measured as A0. Calculate the scavenging rate (%):

[0044] Clearance rate (%) = [1-(A1-A2) / A0] × 100%

[0045] Where A0 is the absorbance of the blank sample, A2 represents the absorbance of the blank reagent, and A1 is the absorbance of the active sample.

[0046] IC of Vc 50 The IC value of DPPH free radical scavenging of mixed and crushed plant extracts is 0.005 mg / mL. 50 The values ​​were 1.88mg / mL and 1.94mg / mL respectively. The results showed that it had good antioxidant effect within 1-3mg / ml. Figure 1 (right) shown.

[0047] (2) Tyrosinase inhibition assay

[0048] Draw the standard curve of β-arbutin, and the sample addition method is shown in Table 1, where A1 represents the blank background group, A2 represents the blank group, A3 represents the sample background group, and A4: the sample group. Calculation:

[0049] Tyrosinase inhibition rate (%) = [1-(A4-A3) / (A2-A1)] × 100%

[0050] Table 1 Reaction solution addition method

[0051]

[0052] The standard curve is: y = 151.54x + 13.988, R 2 =0.9991

[0053] The results showed that the concentration and absorbance of β-arbutin showed a good linear relationship within the range of 0.1 mg / ml to 0.5 mg / ml.

[0054] Determination of tyrosinase inhibition rate: Using the above experimental method, β-arbutin was replaced with mixed plant extracts for determination and calculation.

[0055] The results showed that the tyrosinase inhibition rate of mixed high-quality Tremella extract was 79.63%, and the tyrosinase inhibition rate of mixed broken Tremella extract was 74.27%, indicating that both extracts have whitening effects, and the results are similar, which provides a reference value for the reuse of broken Tremella. It is proved that broken Tremella contains a variety of nutrients and bioactive substances, has a certain utilization value, and can be reprocessed and applied through comprehensive utilization to reduce resource waste and environmental pollution.

[0056] Example 3: Process Optimization of Dictamni Water Extract

[0057] (1) Single factor experiment of Dictamni cortex extract

[0058] The fixed factors were liquid-to-solid ratio of 25:1 (mL / g), extraction temperature of 100℃, extraction time of 2h, and extraction once. The liquid-to-solid ratio of 20:1 (mL / g), 25:1 (mL / g), 30:1 (mL / g), 35:1 (mL / g), and 40:1 (mL / g); extraction temperature of 60℃, 70℃, 80℃, 90℃, and 100℃; extraction time of 1h, 1.5h, 2h, 2.5h, 3.5h, and extraction times of 1, 2, 3, 4, and 5 times were investigated respectively. The polysaccharide content was used as the investigation index to screen the single-factor extraction process. When the liquid-to-solid ratio was 30:1 (mL / g), the polysaccharide content reached a maximum of 37.67%; when the extraction temperature was 80℃, the polysaccharide content reached a maximum of 38.60%; when the extraction time was 2h, the polysaccharide content reached a maximum of 36.23%; when the extraction times were 2 times, the polysaccharide content reached a maximum of 44.74%.

[0059] The optimal extraction conditions of Dictamni cortex extract in single factor experiment were liquid-to-solid ratio of 30:1 (mL / g), extraction time of 2h, extraction temperature of 80℃, extraction times of 2 times and polysaccharide content of 46.04%.

[0060] (2) Response surface experiment of Dictamni cortex extract

[0061] The optimal extraction process under the single factor condition in Example 3 (1) was adopted. The liquid-to-solid ratio, extraction time and extraction temperature were used as independent variables, and the extraction rate of Dictamni polysaccharide was used as the response factor water value. The experimental factor levels are shown in Table 2.

[0062] Table 2. Response surface experiment factor level table

[0063]

[0064] The effects of different levels of liquid-to-solid ratio A, extraction temperature B, and extraction time C on the extraction rate of Dictamni cortex polysaccharides are shown in Table 3.

[0065] Table 3 Response surface analysis results

[0066]

[0067]

[0068] Reference Figure 2 The response surface analysis of the regression model is expressed as the equation:

[0069] Y=44.71+0.11A+0.50B-0.30C-0.22AB-1.17AC-1.21BC-3.06A 2 -3.1

[0070] 5B 2 -5.21C 2 The F value of the model is 20.96, and the corresponding P value is 0.0003, which is significantly less than 0.05. The model is statistically significant. The F value of the lack-of-fit term is 0.81, and the P value is 0.5529, which is significantly greater than 0.05. This confirms that the model is significant, but the degree of lack-of-fit is not significant. The coefficient of determination R of the model 2 Up to 0.9642, the adjusted R 2 The (adj) value is 0.9182. These two indicators show that the model has good fit and credibility. In addition, the coefficient of variation (CV) is 2.81%, which shows the stability and reliability of the experimental results. Therefore, this model can be used as a reliable tool to predict and analyze the extraction rate of Dictamni cortex polysaccharides in the Dictamni cortex water extraction process. The order of influence of the three factors on the extraction rate of Dictamni cortex polysaccharides is: B (extraction temperature)>C (extraction time)>A (liquid-to-solid ratio). After using the regression model analysis, the optimal extraction process parameters were determined: the liquid-to-solid ratio was 30.12:1 (mL / g), the extraction time was 1.98h, the extraction temperature was 80.86℃, and the extraction was repeated twice. Combined with the convenience and operability of the experiment, the optimal extraction process parameters were determined: liquid-to-solid ratio of 30:1 (mL / g), extraction time of 2h, extraction temperature of 81℃, extraction times of 2 times, and the extraction rate was 44.74±1.3%

[0071] Example 4: Preparation of plant extract

[0072] Preparation of Dictamni Cortex Extract: Grind 20 g of Dictamni Cortex with a solid-liquid ratio of 30:1 (mL / g), heat and extract twice at 81°C for 2 hours, filter with 3 layers of gauze, centrifuge at 4800 r / min for 15 minutes, take the top clear liquid, and the polysaccharide content is 44.74%.

[0073] Preparation of Bletilla striata extract: weigh 20g of Bletilla striata powder, add 200ml of water, soak for half an hour, extract for 1.5h, cool and filter; add 120ml of pure water to extract for 1h, cool and filter; add 120ml of water to extract for 0.5h, cool and filter, combine the three filtrates to obtain Bletilla striata extract with a polysaccharide content of 30.3%.

[0074] Preparation of ginseng water extract: Weigh 20 g of ginseng powder and mix with 600 ml of pure water, add into a distillation flask, extract at 60° C. for 2 h, and the ginseng polysaccharide content is 37.9%.

[0075] Preparation of residual tremella extract: Weigh 20 g tremella powder and mix with 800 ml pure water, add into a distillation flask, extract at 80°C for 3.5 h, the tremella polysaccharide content is 7.2%.

[0076] The polysaccharide obtained by the hot water extraction method of the present invention has a high content, is convenient and easy to obtain, can be directly added for preparation, saves costs, and is conducive to industrial production.

[0077] Example 5: Single factor and response surface experiment of main ingredients and auxiliary materials of facial cream extract

[0078] Single factor experiment of extract main ingredients: evaluate the effect of four different extract addition amounts on the antioxidant properties of the cream to determine the optimal ratio of the four plant extracts added to the cream formula. Using the method in Example 2 and taking ABTS free radical scavenging activity as an indicator, a single factor experiment was used to determine the amount of Dictamni, Bletilla striata, ginseng, and broken Tremella extracts, and finally determine the ratio of the mixed extracts.

[0079] The fixed factors were 1 mL of ginseng extract, 2 mL of bletilla striata extract and 2 mL of difficile cortex extract, and the added amount of broken tremella was 2.25 mL. The dosages of the four extracts were investigated respectively, namely 2 mL, 2.25 mL, 2.5 mL, 2.75 mL and 3 mL of tremella extract; 0.5 mL, 1 mL, 1.5 mL, 2 mL and 2.5 mL of difficile cortex extract; 0.5 mL, 1 mL, 1.5 mL, 2 mL and 2.5 mL of bletilla striata extract; 0.5 mL, 1 mL, 1.5 mL, 2 mL and 2.5 mL of ginseng extract were centrifuged and measured at 734 nm, with free radical scavenging activity as the assessment indicator. When the amount of broken Tremella fuciformis added was 2.25 mL, the highest clearance rate was 43.18%; when the amount of Dictamni cortex was 2 mL, the highest clearance rate was 41.20%; when the amount of Bletilla striata was 2 mL, the highest clearance rate was 40.02%; when the amount of Ginseng was 1.5 mL, the highest clearance rate was 40.30%. Figure 3 shown.

[0080] In summary, when the ratio of the four extracts of broken Tremella fuciformis, Dictamni cortex, Bletilla striata and ginseng is 2.25:2:2:1.5, the removal rate of the mixed extract is the best.

[0081] Selection of the dosage of mixed extract: Prepare the mixed extract according to the optimal ratio obtained above, and then take the mixed extract of 4 plants at a ratio of 2%, 3%, 4%, 5%, and 6%. The antioxidant activity of each mixed extract dilution was evaluated by ABTS free radical scavenging method, and the odor of the extract was scored. The scoring criteria are shown in Table 4. The results are shown in Figure 4 As shown in the figure, the removal rate first increases with the increase of the mass fraction of the extract, tends to be flat, and then increases with an upward trend. The odor score becomes lower as the mass fraction of the extract increases. When the amount of the extract is 3%, 4%, and 5%, the removal rates are 16.0%, 22.29%, and 22.57%, respectively, and the odor scores are 9, 8.7, and 6.1, respectively. Based on the comprehensive removal rate and odor score results, 4% of the extract is selected as the optimal amount.

[0082] Table 4 Odor Rating Table

[0083]

[0084] Excipient single factor experiment dosage screening: 6 factors are glyceryl stearate, cetearyl alcohol, stearic acid, coconut oil, glycerol and 1,2-propylene glycol. The fixed value is 5.0% (mass fraction, all the following are mass fractions) of glyceryl stearate, 1% of cetearyl alcohol, 4% of stearic acid, 20% of coconut oil, 8% of glycerol and 1% of 1,2-propylene glycol to prepare the cream.

[0085] Glyceryl stearate with different mass fractions (3.0%, 4.0%, 5.0%, 6.0%, 7.0%) was selected for the experiment to examine the appearance and stability of the finished cream. The sensory evaluation comprehensive scoring standard was referred to Table 5, and the optimal dosage of glyceryl stearate was determined based on the sensory comprehensive scoring. Figure 5 As shown in the figure, when the content of glyceryl stearate is 4.0%, 5.0% and 6.0%, the sensory scores of the cream are 87.9 points, 94 points and 92.6 points respectively. When the content of glyceryl stearate is 5.0%, the sensory score of the cream is the highest.

[0086] Different mass fractions of cetearyl alcohol (0.5%, 1.0%, 1.5%, 2.0%, 2.5%) were selected for the experiment, and the method was the same as above. Figure 5 As shown, when the content of cetearyl alcohol is 1.0%, 1.5%, and 2.0%, the sensory scores of the cream are 89.1 points, 93.7 points, and 90 points respectively. When the content of cetearyl alcohol is 1.5%, the sensory score of the cream is the highest.

[0087] Different mass fractions of stearic acid (2.0%, 3.0%, 4.0%, 5.0%, 6.0%) were selected for the experiment, and the method was the same as above. Figure 5 As shown, when the stearic acid content is 4.0%, 5.0%, and 6.0%, the sensory scores of the cream are 92.6 points, 93.6 points, and 89.7 points respectively. When the stearic acid content is 5.0%, the sensory score of the cream is the highest.

[0088] Coconut oil with different mass fractions (16%, 18%, 20%, 22%, 24%) was selected for the experiment, and the greasiness of the finished cream was examined and scored. The sensory evaluation comprehensive scoring standard was referred to Table 5 to determine the optimal amount of coconut oil. Figure 5 As shown in the figure, when the mass fraction of coconut oil is 18%, 20%, and 22%, the sensory scores of the cream are 84.8, 90.1, and 89.1 respectively. When the mass fraction of coconut oil is 20%, the sensory score of the cream is the highest.

[0089] Glycerin with different mass fractions (6%, 7%, 8%, 9%, 10%) was selected for experiment to examine the appearance, stability and moisturizing rate of the finished cream and determine the optimal amount of glycerin. Figure 5 As shown in the figure, when the mass fraction of glycerol is 7%, 8%, and 9%, the moisturizing rates are 92.48%, 93.33%, and 93.3% respectively. When the mass fraction of glycerol is 8%, the moisturizing rate of the cream is the highest.

[0090] Different mass fractions of 1,2-propylene glycol (0.5%, 1.0%, 1.5%, 2.0%, 2.5%) were selected for experiments to investigate the moisturizing rate of the finished cream and determine the optimal amount of 1,2-propylene glycol. Figure 5As shown, when the mass fraction of 1,2-propylene glycol is 1.0%, 1.5%, and 2.0%, the moisturizing rates are 92.72%, 93.13%, and 92.85%, respectively. When the mass fraction of 1,2-propylene glycol is 1.5%, the moisturizing rate of the cream is the highest.

[0091] Table 5 Sensory evaluation table

[0092]

[0093]

[0094] In the comprehensive evaluation of skin care products, sensory, moisturizing rate, stability and smell are four key evaluation indicators. The weight distribution of these indicators is 20% for sensory, 40% for moisturizing, 20% for stability and 20% for smell. Moisturizing rate is the core indicator for evaluating the effect of skin care products. The moisturizing effect is directly related to whether the product can meet the basic skin care needs of consumers, so it occupies the highest weight in the comprehensive evaluation; sensory score and smell score are important factors for consumers to choose skin care products, which directly affect consumers' usage experience and market acceptance of products; stability can ensure that its ingredients do not deteriorate during the validity period, ensuring the safety and effectiveness of consumers when using, so sensory, smell and stability occupy an equally important weight.

[0095] Response surface experiment of facial cream: After the single factor investigation of main ingredients and auxiliary ingredients, the mixed extract of main ingredients and three auxiliary ingredients with greater influence were selected for response surface design. Glyceryl stearate, coconut oil, glycerin and mixed extract were used as independent variables, and the comprehensive score of sensory score, moisturizing rate, stability and odor was used as the response value. The experimental factor levels are shown in Table 6.

[0096] Table 6 Response surface experiment factor level table

[0097]

[0098] By establishing a mathematical model, the amount of the main ingredients and auxiliary ingredients of the cream can be effectively predicted, and the optimal cream formula can be finally obtained. The results of the response surface experiment are shown in Table 7.

[0099] Table 7 Response surface analysis experimental results

[0100]

[0101]

[0102] The response surface results are as follows Figure 6It can be seen that glyceryl stearate, coconut oil, glycerin, and extract interact with each other, and the response surface is convex, indicating that with the increase of the proportion of glyceryl stearate, coconut oil, and glycerin, the comprehensive score of the cream shows a trend of first rising and then falling, so it can be judged that the comprehensive score of the cream has a strong correlation with these factors. In summary, according to the comprehensive analysis results of the cream, the mass score of each factor is obtained, and the percentage by weight is calculated, and the mixed plant extract 4%, glyceryl stearate 5.0%, cetearyl alcohol 1.5%, stearic acid 5%, coconut oil 20%, glycerin 8%, 1,2-propylene glycol 1.5%, and the final cream formula is shown in Table 8.

[0103] Table 8 Final formula of facial cream

[0104]

[0105]

[0106] Embodiment 6: facial cream preparation method

[0107] Referring to the ingredients of each phase in Table 8 of Example 5, a facial cream was prepared. The preparation process is as follows: Figure 7 .

[0108] S1. Place the mixed extract of the four extracts in a centrifuge and centrifuge at 5000r / min for 30min. After centrifugation, take the supernatant for later use.

[0109] S2. Place phase A and phase B in the same beaker, heat in a 75°C constant temperature water bath to melt them and stir evenly, and keep the temperature constant for 5 minutes.

[0110] S3. Place phase C and phase D in the same beaker, heat in a 75°C constant temperature water bath to melt them and stir evenly, and keep the temperature constant for 5 minutes.

[0111] S4. Slowly add the melted and kept warm phases C and D into the completely melted phases A and B. When the water phase is completely poured into the oil phase, take it out of the water bath. At this time, the solution is milky white with a small amount of oil droplets on top. Homogenize and emulsify at a speed of 3000r / min for 5-15min.

[0112] S5. Add phase E, place on a magnetic heating stirrer, and stir at 3500 r / min for 10 min for defoaming treatment to obtain a facial cream, which is used for the evaluation of Examples 7-8.

[0113] Example 7: Evaluation of facial cream functionality

[0114] (1) Determination of moisture retention rate

[0115] The moisturizing rate was determined by weighing method. A transparent 10×10 cm board was used as a carrier. A 2×5 cm medical tape was pasted on it. The weight was weighed and the mass was accurately recorded. 0.2 g of Dictamni cream and the commonly used sample 1 (the aqueous phase includes water, glycerin, ginseng extract, etc.; the oil phase is mineral oil; the emulsifiers include stearyl alcohol, potassium lauryl phosphate, cetyl alcohol, glyceryl stearate, etc.) and sample 2 (main ingredients: the aqueous phase includes water, glycerin, summer truffle extract, 1, 2-pentanediol, etc.; the oil phase is olive oil; the emulsifiers include isopropyl isostearate, stearyl alcohol, cetyl alcohol, cetearyl alcohol, etc.) were evenly spread on the tape, weighed, and the data was recorded. A saturated sodium acetate solution was prepared to create a relative humidity of 60%. The solution was placed in a desiccator. The data were recorded at 1, 2, 3, 4, 5, 6, 8, 12, and 24 h, and the moisturizing rate was calculated. The solution was repeated 3 times and the average value was calculated.

[0116] Moisture retention rate (%) = m2 ÷ m1 × 100%

[0117] Where: m1 is the mass of the sample before drying; m2 is the mass of the sample after drying.

[0118] The comparison of the moisturizing rates of the white fresh skin cream and the commonly used samples 1 and 2 on the market is shown in Table 9.

[0119] Table 9 Moisture Retention Rate Data

[0120]

[0121]

[0122] The results show that before 5 hours, the moisturizing rate of the cream is higher than sample 1 and lower than sample 2. After 5 hours, the cream is better than the two commercially available samples 1 and 2. Figure 8 shown.

[0123] (2) Skin water and oil determination

[0124] 10 volunteers with oily, neutral and dry skin were selected, aged 20 to 55 years old, healthy and with good judgment ability. A 1g sample test was conducted on the same position of the face of 30 volunteers, and the white fresh skin cream and the two commonly used creams on the market were compared on the left and right faces respectively. The skin was tested before and after the cream with a skin tester, and the comprehensive scores of skin moisture content, oil content and elasticity were obtained by comparison. During the test, the cleanliness of the volunteers' faces was ensured, and no other daily chemical products were mixed to avoid affecting the experimental results. According to the instructions of the testing instrument, the water and oil content of different skins in summer are shown in Table 10.

[0125] Table 10 Water and oil content of different skin types in summer

[0126]

[0127] According to the above skin water and oil test method, a skin detector was used to measure the comprehensive scores of water, oil and elasticity of three different skin types. The measured results are shown in Table 11. The data in the table are for the same skin type as one group, with ten people in each group. The scores of ten people were measured by the instrument, and the highest and lowest scores were selected as the interval range.

[0128] Table 11 Skin tester results

[0129]

[0130] According to the data results of the skin detector, it can be concluded that the comprehensive score of water, oil and elasticity of the cream is better than that of sample 2, and is not much different from sample 1, and has a better water-oil balance effect.

[0131] Example 8: Skin irritation test

[0132] Twenty volunteers in good physical condition were selected, and the area behind the ears of the volunteers was about 4cm 2 Apply the sample to the area of ​​the test sample, once in the morning and once in the evening, and keep applying for 7 days. Record the skin condition after application, whether there is rash, edema, itching, etc. Record and rate the volunteers. During the experiment, ensure that no other daily chemical products are used on the sample area.

[0133] The results showed that no rashes, edema, itching, etc. were found after using the cream, which is highly safe. This is because the pure natural plant extract is prepared by hot water extraction, which is safe and non-irritating.

Claims

1. A facial cream formula using natural plant extracts as active ingredients, characterized in that: It is composed of a combination of four natural plant ingredients, namely, broken Tremella fuciformis, Dictamni cortex, Bletilla striata, and ginseng extract, and five phases A, B, C, D, and E, wherein by weight percentage: Phase A contains the following ingredients: 5% glyceryl stearate, 1.5% cetearyl alcohol, 5% stearic acid; Phase B contains the following ingredients: 20.5% coconut oil; Phase C contains the following components: 8% glycerin, 1,2-propylene glycol 1.5%, mixed extract 4%, Phase D contains the following components: 0.5% D-panthenol, 0.5% allantoin, 0.5% tocotrienol; Phase E contains the following components: 0.5% triethanolamine, 0.2% phenoxyethanol; Add ultrapure water to make up to 100 g.

2. A facial cream formula using natural plant extracts as active ingredients, characterized in that: 20g of Dictamni cortex was crushed, the liquid-to-solid ratio was 30:1 (mL / g), heated and extracted twice at 81°C for 2 hours, filtered through 3 layers of gauze, centrifuged at 4800rpm for 15 minutes, and the supernatant was taken, the polysaccharide content was 44.74%.

3. A facial cream formula using natural plant extracts as active ingredients, characterized in that: The optimal volume mixing ratio of the four extracts in the facial cream of claim 1 is shredded Tremella: Dictamni: Bletilla striata: Ginseng = 2.25:2:2:1.

5.

4. A facial cream formula using natural plant extracts as active ingredients, characterized in that: According to claim 3, the facial cream extract is prepared by respectively using high-quality Tremella fuciformis and broken Tremella fuciformis with Dictamni cortex, Bletilla striata and Ginseng to prepare mixed and mixed extracts, and the IC of DPPH free radical scavenging of the mixed and mixed extracts is 50 The values ​​were 1.88 mg / mL and 1.94 mg / mL, respectively. The IC 50 The values ​​were 1.29 mg / mL and 1.40 mg / mL respectively, and the tyrosinase inhibition rates were 79.63% and 74.27% respectively. The extracts have antioxidant and whitening effects.

5. A facial cream formula using natural plant extracts as active ingredients, characterized in that: The amount of the facial cream ingredients according to claim 1 is 4% of the mixed plant extract, and the excipients are 5% of glyceryl stearate, 1.5% of cetearyl alcohol, 5% of stearic acid, 20.5% of coconut oil, 8% of glycerol, 1,2-propylene glycol, 0.5% of D-panthenol, 0.5% of allantoin, 0.5% of tocotrienol, 0.5% of triethanolamine, 0.2% of phenoxyethanol, and ultrapure water is added to 100g.

6. A facial cream formula using natural plant extracts as active ingredients, characterized in that: S1. Place the four extracts in a centrifuge and centrifuge at 5000r / min for 30min. After the centrifugation, take the supernatant for later use; S2. Place phase A and phase B in the same beaker, heat in a 75°C constant temperature water bath to melt and stir evenly, and keep the temperature constant for 5 minutes; S3. Place phase C and phase D in the same beaker, heat in a 75°C constant temperature water bath to melt and stir evenly, and keep the temperature constant for 5 minutes; S4. Slowly add the melted and heat-maintained phases C and D to the completely melted phases A and B. When the aqueous phase is completely poured into the oil phase, remove it from the water bath. At this time, the solution is milky white with a small amount of oil droplets on top. Homogenize and emulsify at a speed of 3000r / min for 5-15min; S5. Add phase E, place on a magnetic heating stirrer, and stir at 3500 r / min for 10 min to defoam, to obtain a facial cream.

7. A facial cream formula using natural plant extracts as active ingredients, characterized in that: According to the functional evaluation of the natural plant facial cream according to claim 1, the moisturizing rate of the facial cream after being placed for 24 hours is 41.88%, and the moisturizing effect of the facial cream is good; the skin water and oil determination score is between 60-85 points, and the skin water and oil balancing effect is good.

8. A facial cream formula using natural plant extracts as active ingredients, characterized in that: Choosing broken Tremella fuciformis as raw material can achieve the purpose of saving costs and promoting resource recycling.

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