Digital phantom intensity calculation method using illumination light feedback, electronic equipment and medium

Abstract

The invention provides a digital phantom intensity calculation method based on illumination light feedback, electronic equipment and a medium. The digital phantom intensity calculation method comprises the following steps: acquiring first excitation power P1 and first excitation wavelength A (lambda) of a fluorescence acquisition system; obtaining the fluorescence molecule concentration, the fluorescence spectrum curve, the excitation efficiency curve E (lambda) and the fluorescence emission intensity of the fluorescence molecule sample; acquiring second excitation power P2 and second excitation wavelength B (lambda) of the to-be-tested equipment; setting a fluorescence concentration value c, and obtaining a fluorescence emission spectrum curve corresponding to the to-be-detected equipment according to a relation W (c) between the fluorescence center wavelength and the fluorescence concentration; calculating a fluorescence emission ratio eta (c) based on the second excitation wavelength B (lambda) and the excitation efficiency curve E (lambda) of the to-be-detected equipment, the first excitation wavelength A (lambda) of the fluorescence acquisition system, the second excitation power P2 of the to-be-detected equipment and the first excitation power P1 of the fluorescence acquisition system; and multiplying the fluorescence emission ratio eta (c) by the fluorescence emission intensity I (c) at any point in the space to obtain the fluorescence emission intensity of the to-be-detected equipment at the position.

Description

Technical Field

[0001] The invention relates to the technical field of bioluminescence imaging, and in particular to a method, electronic equipment and medium for calculating the intensity of a digital phantom using illumination light feedback. Background Art

[0002] In the current technological field, accurate restoration of fluorescence properties is crucial for many applications, including biomedical imaging, materials science, environmental monitoring, and industrial detection. However, traditional digital phantom restoration methods are often limited by the fixed parameters of the fluorescence acquisition system and the inherent properties of fluorescent molecules, which may lead to deviations in measurement results under different devices or under different conditions. In addition, due to the complexity of fluorescence signals, it has always been a challenge to achieve quantitative calculation of fluorescence intensity. For example, existing phantom restoration methods only consider the distribution of light, without considering factors such as wavelength and excitation efficiency, and cannot accurately restore the light field information of the digital phantom. Summary of the invention

[0003] The purpose of the present invention is to address the deficiencies of the prior art and provide a method, electronic device and medium for calculating the intensity of a digital phantom using illumination light feedback.

[0004] In a first aspect, an embodiment of the present invention provides a method for calculating the intensity of a digital phantom using illumination light feedback, the method comprising:

[0005] Acquire a first excitation power P1 and a first excitation wavelength A(λ) of a fluorescence collection system;

[0006] Obtain the fluorescent molecule concentration, fluorescence spectrum curve, excitation efficiency curve E(λ) and fluorescence emission intensity of the fluorescent molecule sample;

[0007] Obtaining a second excitation power P2 and a second excitation wavelength B(λ) of the device under test;

[0008] Set the fluorescence concentration value c, and obtain the fluorescence emission spectrum curve corresponding to the device under test from the relationship between the fluorescence center wavelength and the fluorescence concentration W(c);

[0009] Calculate the fluorescence emission ratio η(c) based on the second excitation wavelength B(λ) of the device under test, the excitation efficiency curve E(λ), the first excitation wavelength A(λ) of the fluorescence collection system, the second excitation power P2 of the device under test, and the first excitation power P1 of the fluorescence collection system;

[0010] The fluorescence emission intensity of the device under test at that position is calculated by multiplying the fluorescence emission ratio η(c) by the fluorescence emission intensity I(c) at any point in space.

[0011] In a second aspect, an embodiment of the present invention provides an electronic device, comprising a memory and a processor, wherein the memory is coupled to the processor; wherein the memory is used to store program data, and the processor is used to execute the program data to implement the above-mentioned digital phantom intensity calculation method using illumination light feedback.

[0012] In a third aspect, an embodiment of the present invention provides a computer-readable storage medium having a computer program stored thereon, which, when executed by a processor, implements the above-mentioned method for calculating the intensity of a digital phantom using illumination light feedback.

[0013] In a fourth aspect, an embodiment of the present invention provides a computer program product, including a computer program / instruction, which, when executed by a processor, implements the above-mentioned method for calculating the intensity of a digital phantom using illumination light feedback.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] The present invention provides a method for calculating the intensity of a digital phantom using illumination light feedback. The present invention not only takes into account a first excitation power, a first laser two-dimensional plane distribution and a first excitation wavelength of a fluorescence acquisition system, but also integrates the characteristics of a fluorescent molecule sample including a fluorescent molecule concentration, a fluorescence spectrum curve, an excitation efficiency curve and a fluorescence emission intensity. The present invention calculates the fluorescence emission intensity of a device to be tested according to the excitation and emission principle of fluorescence by detecting a second excitation power, a second laser two-dimensional plane distribution and a second excitation wavelength corresponding to the device to be tested. The present invention makes it possible to map from a physical space to a digital space, thereby reproducing the fluorescence characteristics in a digital phantom, and significantly improving its accuracy and reliability. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the accompanying drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings in the following description are only some embodiments of the present invention. For ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without paying creative labor.

[0017] Figure 1 is a flow chart of a method for calculating the intensity of a digital phantom using illumination light feedback provided by an embodiment of the present invention;

[0018] Figure 2 is a schematic diagram of a fluorescence collection system provided by an embodiment of the present invention;

[0019] Figure 3 is a schematic diagram of an image calibration process of a fluorescence acquisition system provided in an embodiment of the present invention;

[0020] Figure 4 is a schematic diagram of a first laser two-dimensional plane distribution and a first excitation wavelength corresponding to the fluorescence collection system provided in an embodiment of the present invention;

[0021] Figure 5 It is a schematic diagram of a fluorescence spectrum curve and an excitation efficiency curve corresponding to a fluorescent molecule provided in an embodiment of the present invention;

[0022] Figure 6 is a schematic diagram of fluorescence spectrum curve fitting corresponding to the fluorescent molecule provided in an embodiment of the present invention;

[0023] Figure 7 is a schematic diagram of the fluorescence emission intensity fitting corresponding to the fluorescent molecules provided in an embodiment of the present invention;

[0024] Figure 8 is a schematic diagram of the second laser two-dimensional plane distribution and the second excitation wavelength corresponding to the device under test provided by an embodiment of the present invention;

[0025] Fig. 9 is a schematic diagram of the fluorescence emission intensity corresponding to the device under test provided by an embodiment of the present invention;

[0026] Fig.10 is a schematic diagram of an electronic device provided by an embodiment of the present invention. DETAILED DESCRIPTION

[0027] Exemplary embodiments will be described in detail herein, examples of which are shown in the accompanying drawings. When the following description refers to the drawings, the same numbers in different drawings represent the same or similar elements unless otherwise indicated. The embodiments described in the following exemplary embodiments do not represent all embodiments consistent with the present invention. Instead, they are merely examples of devices and methods consistent with some aspects of the present invention as detailed in the appended claims.

[0028] The terms used in the present invention are only for the purpose of describing specific embodiments and are not intended to limit the present invention. The singular forms "a", "the" and "the" used in the present invention and the appended claims are also intended to include plural forms unless the context clearly indicates other meanings. It should also be understood that the term "and / or" used herein refers to and includes any or all possible combinations of one or more associated listed items.

[0029] It should be understood that although the terms first, second, third, etc. may be used in the present invention to describe various information, these information should not be limited to these terms. These terms are only used to distinguish the same type of information from each other. For example, without departing from the scope of the present invention, the first information may also be referred to as the second information, and similarly, the second information may also be referred to as the first information. Depending on the context, the word "if" as used herein may be interpreted as "at the time of" or "when" or "in response to determining".

[0030] The present invention is described in detail below in conjunction with the accompanying drawings. In the absence of conflict, the features of the following embodiments and implementations can be combined with each other.

[0031] like Figure 1 As shown, an embodiment of the present invention provides a method for calculating the intensity of a digital phantom using illumination light feedback, the method comprising the following steps:

[0032] Step S1, obtaining a first excitation power P1, a first laser two-dimensional plane distribution, and a first excitation wavelength A(λ) of a fluorescence collection system.

[0033] Specifically, in this example, Figure 2 As shown, a black and white camera and a lens group are placed and assembled, and within the imaging distance range, a two-dimensional plane distribution of the first laser of the illumination light is detected by a two-dimensional plane detector;

[0034] At a fixed central position on the imaging surface, a first excitation power P1 of the illumination light is recorded by an optical power meter;

[0035] At a fixed central position of the imaging plane, the first excitation spectrum of the illumination light is recorded by a spectrometer fiber optic probe, thereby obtaining a first excitation wavelength A(λ).

[0036] Step S2, obtaining the fluorescent molecule concentration, fluorescence spectrum curve, excitation efficiency curve E(λ) and fluorescence emission intensity of the fluorescent molecule sample.

[0037] The process of obtaining the excitation efficiency curve E(λ) includes:

[0038] The fluorescence collection system irradiates the fluorescent molecules with a series of first excitation wavelengths A(λ), records the fluorescence emission power, and fits the fluorescence emission power with the first excitation wavelength A(λ) to obtain the relationship function between the fluorescence emission power and the first excitation wavelength A(λ), namely, the excitation efficiency curve E(λ).

[0039] Step S3, obtaining a second excitation power P2, a second laser two-dimensional plane distribution, and a second excitation wavelength B(λ) of the device under test.

[0040] Specifically, in this example, a black-and-white camera and a filter lens group with a bandwidth of 780 nm are placed and assembled, and within the imaging distance range, a two-dimensional plane distribution of the second laser of the illumination light is detected by a two-dimensional plane detector;

[0041] At a fixed central position on the imaging surface, a second excitation power P2 of the illumination light is recorded by an optical power meter;

[0042] At a fixed central position of the imaging surface, the second excitation spectrum of the illumination light is recorded by the optical fiber probe of the spectrometer, thereby obtaining the second excitation wavelength B(λ).

[0043] Step S4, setting the fluorescence concentration value c, and obtaining the fluorescence emission spectrum curve corresponding to the device to be tested from the relationship W(c) between the fluorescence center wavelength and the fluorescence concentration.

[0044] The process of obtaining the relationship W(c) between the fluorescence center wavelength and the fluorescence concentration includes:

[0045] A series of fluorescent molecule samples with concentration gradients are prepared; a spectrometer is used to fix the excitation wavelength at 780 nm, and the series of gradient fluorescent molecule solutions are spectrally scanned in sequence, thereby detecting and obtaining a fluorescence spectrum curve, extracting the fluorescence center wavelength of the fluorescence spectrum curve, and obtaining discrete sampling points of the center wavelength of the fluorescence spectrum curve along with the concentration of the fluorescent molecule;

[0046] The fluorescence center wavelength and the fluorescence concentration are fitted to obtain the relationship W(c) between the fluorescence center wavelength and the fluorescence concentration.

[0047] Step S5, calculating the fluorescence emission ratio η(c) based on the second excitation wavelength B(λ) of the device under test, the excitation efficiency curve E(λ), the first excitation wavelength A(λ) of the fluorescence collection system, the second excitation power P2 of the device under test, and the first excitation power P1 of the fluorescence collection system.

[0048] Calculate the integral of the first excitation wavelength A(λ) of the fluorescence collection system and the excitation efficiency curve E(λ) to obtain the relative fluorescence excitation amount corresponding to the fluorescence collection system;

[0049] Calculate the integral of the second excitation wavelength B(λ) of the device under test and the excitation efficiency curve E(λ) to obtain the relative fluorescence excitation amount corresponding to the device under test;

[0050] The ratio of the relative fluorescence excitation amount corresponding to the fluorescence collection system to the relative fluorescence excitation amount corresponding to the device to be tested is taken as the relative fluorescence emission ratio;

[0051] In order to obtain an absolute quantified fluorescence emission ratio, the relative fluorescence emission ratio needs to be normalized to the excitation power; the ratio of the second excitation power P2 of the device under test and the first excitation power P1 of the fluorescence acquisition system is calculated, and the ratio is multiplied by the relative fluorescence emission ratio to obtain the absolute fluorescence emission ratio η(c).

[0052] Furthermore, the expression is as follows:

[0053]

[0054] Step S6, multiplying the fluorescence emission ratio η(c) by the fluorescence emission intensity I(c) at any point in space to obtain the fluorescence emission intensity of the device under test at that position, that is, I(c)η(c).

[0055] Alternatively, a two-dimensional plane fluorescence emission intensity change is obtained according to the two-dimensional plane distribution of the first laser and the two-dimensional plane distribution of the second laser.

[0056] The process of obtaining the fluorescence emission intensity I(c) at any point in space includes:

[0057] Under the conditions of the first excitation power P1 and the first excitation wavelength A(λ) of the fluorescence collection system, the fluorescence emission intensity of a series of fluorescent molecule samples with a concentration gradient is detected to obtain discrete sampling points of fluorescence emission intensity versus fluorescent molecule concentration. The fluorescent molecule concentration and fluorescence emission intensity are fitted to obtain the fluorescence emission intensity I(c) at any point in space.

[0058] The process of obtaining the change of the two-dimensional plane fluorescence emission intensity according to the two-dimensional plane distribution of the first laser and the two-dimensional plane distribution of the second laser includes:

[0059] According to the fluorescence emission intensity I(c) at any point in space, the fluorescence emission intensity in two-dimensional space is obtained and recorded as I1(c, x, y);

[0060] The first laser two-dimensional plane distribution of the fluorescence collection system is recorded as D1 (x, y), and the second laser two-dimensional plane distribution of the device to be tested is recorded as D2 (x, y); then the change of the two-dimensional plane fluorescence emission intensity is The digital phantom strength I2(c, x, y) = η(c, x, t)I1(c, x, y).

[0061] Example 1

[0062] This embodiment provides a digital phantom calculation case based on the above-mentioned digital phantom intensity quantitative calculation method using illumination light feedback.

[0063] Step S1, building a fluorescence collection system.

[0064] The excitation of the fluorescence acquisition system was characterized. In the fluorescence acquisition system, a positive fluorescence imaging setting was adopted. The first grayscale camera 1 was placed at a distance of 60 mm from the sample, and an 850 nm filter was added in front of the camera to detect the fluorescence emission intensity of indocyanine green. At the same time, a spectrometer was used to detect the fluorescence spectrum curve, excitation efficiency curve, and excitation wavelength. Figure 3 As shown, the second grayscale camera 2 is placed near the first grayscale camera 1, and the spatial position of the image is calibrated to the first grayscale camera 1; a 780nm color filter is added in front of the second grayscale camera 2 to detect the first laser two-dimensional plane distribution of the excitation light; and a 780nm calibrated optical power meter is used to detect the first excitation power of the excitation light. Figure 4 It shows that the first excitation power corresponding to the fluorescence collection system is 5.69 W, and also provides a schematic diagram of the first laser two-dimensional plane distribution and the first excitation wavelength.

[0065] Step S2, preparing a series of gradient indocyanine green solutions.

[0066] The concentration of the indocyanine green aqueous solution includes 1, 1 / 2, 1 / 4, 1 / 8, 1 / 16 mg / ml, etc., and each concentration is separated by 2 times; the fluorescence acquisition system is used to detect the fluorescence of the sample, and under the excitation of the 780nm laser, the first grayscale camera 1 records the discrete sample points where the fluorescence emission intensity changes with the concentration; then, the function fitting is performed on these discrete sample points to obtain the functional relationship between the fluorescence emission intensity of indocyanine green and the concentration, such as Figure 7 shown.

[0067] The fluorescence spectrum curve of the sample is detected using a fluorescence acquisition system. Under the excitation of a 780nm laser, the spectrometer records the discrete sample points of the fluorescence emission spectrum that changes with concentration. Then, the central wavelength of the discrete sample points is extracted, and a function fitting is performed on the central wavelength to obtain the functional relationship between the fluorescence emission spectrum of indocyanine green and the concentration, as shown in Figure 6 shown.

[0068] The fluorescence acquisition system is used to detect the excitation efficiency curve of the sample. Under the excitation light of 700nm to 800nm, the spectrometer records the discrete sample points of the fluorescence emission spectrum changing with the excitation light wavelength; then the discrete sample points are integrated and the integration results are normalized to obtain the functional relationship of the excitation efficiency curve of indocyanine green changing with the excitation wavelength, such as Figure 5 shown.

[0069] Step S3, characterize the excitation of the device under test, such as Figure 8Place a white diffuse reflection cardboard on the sample plane, turn on the excitation illumination of the device to be tested, use the second grayscale camera 2 to detect the second laser two-dimensional plane distribution of the excitation light, use the spectrometer to detect the second excitation wavelength of the excitation light, and use the optical power meter to detect the second excitation power of the excitation light.

[0070] Step S4, calculating the data obtained by the above detection, specifically:

[0071] The fluorescence concentration value c is set, and the fluorescence emission spectrum curve corresponding to the device under test is obtained from the relationship W(c) between the fluorescence center wavelength and the fluorescence concentration.

[0072] The fluorescence emission ratio η(c) is calculated based on the second excitation wavelength B(λ) of the device under test, the excitation efficiency curve E(λ), the first excitation wavelength A(λ) of the fluorescence collection system, the second excitation power P2 of the device under test, and the first excitation power P1 of the fluorescence collection system. The fluorescence emission ratio η(c) is multiplied by the fluorescence emission intensity I(c) at any point in space to obtain the fluorescence emission intensity of the device under test at that position, which is I(c)η(c), as follows: Fig. 9 shown.

[0073] Accordingly, the present application also provides an electronic device, comprising: one or more processors; a memory for storing one or more programs; when the one or more programs are executed by the one or more processors, the one or more processors implement the above-mentioned digital phantom intensity calculation method using illumination light feedback. Fig.10 As shown in FIG. 1 , a hardware structure diagram of any device with data processing capability for the method for calculating the intensity of a digital phantom using illumination light feedback provided by an embodiment of the present invention is shown in FIG. Fig.10 In addition to the processor, memory and network interface shown, any device with data processing capability in which the apparatus in the embodiment is located may also include other hardware, generally based on the actual functions of the device with data processing capability, which will not be described in detail.

[0074] Accordingly, the present application also provides a computer-readable storage medium on which computer instructions are stored, and when the instructions are executed by a processor, the digital phantom intensity calculation method using illumination light feedback as described above is implemented. The computer-readable storage medium can be an internal storage unit of any device with data processing capabilities described in any of the aforementioned embodiments, such as a hard disk or a memory. The computer-readable storage medium can also be an external storage device, such as a plug-in hard disk, a smart memory card (Smart Media Card, SMC), an SD card, a flash card (Flash Card), etc. equipped on the device. Further, the computer-readable storage medium can also include both an internal storage unit of any device with data processing capabilities and an external storage device. The computer-readable storage medium is used to store the computer program and other programs and data required by any device with data processing capabilities, and can also be used to temporarily store data that has been output or is to be output.

[0075] The above embodiments are only used to illustrate the design ideas and features of the present invention, and their purpose is to enable those skilled in the art to understand the content of the present invention and implement it accordingly. The protection scope of the present invention is not limited to the above embodiments. Therefore, any equivalent changes or modifications made based on the principles and design ideas disclosed by the present invention are within the protection scope of the present invention.

Claims

1. A method for calculating the intensity of a digital phantom using illumination light feedback, characterized in that: The method specifically comprises the following steps: Acquire a first excitation power P1 and a first excitation wavelength A(λ) of a fluorescence collection system; Obtain the fluorescent molecule concentration, fluorescence spectrum curve, excitation efficiency curve E(λ) and fluorescence emission intensity of the fluorescent molecule sample; Obtaining a second excitation power P2 and a second excitation wavelength B(λ) of the device under test; Set the fluorescence concentration value c, and obtain the fluorescence emission spectrum curve corresponding to the device under test from the relationship between the fluorescence center wavelength and the fluorescence concentration W(c); Calculate the fluorescence emission ratio η(c) based on the second excitation wavelength B(λ) of the device under test, the excitation efficiency curve E(λ), the first excitation wavelength A(λ) of the fluorescence collection system, the second excitation power P2 of the device under test, and the first excitation power P1 of the fluorescence collection system; The fluorescence emission intensity of the device under test at that position is calculated by multiplying the fluorescence emission ratio η(c) by the fluorescence emission intensity I(c) at any point in space.

2. The method for calculating the intensity of a digital phantom using illumination light feedback according to claim 1, characterized in that: The process of obtaining the relationship between the fluorescence center wavelength and the fluorescence concentration W(c) includes: A series of fluorescent molecular samples with concentration gradients are configured, the fluorescence spectrum curve is detected, and the fluorescence center wavelength of the fluorescence spectrum curve is extracted; The fluorescence center wavelength and the fluorescence concentration are fitted to obtain the relationship W(c) between the fluorescence center wavelength and the fluorescence concentration.

3. The method for calculating the intensity of a digital phantom using illumination light feedback according to claim 1, characterized in that: The process of obtaining the excitation efficiency curve E(λ) includes: The fluorescence collection system irradiates the fluorescent molecules with a series of first excitation wavelengths A(λ), records the fluorescence emission power, and fits the fluorescence emission power with the first excitation wavelength A(λ) to obtain the relationship function between the fluorescence emission power and the first excitation wavelength A(λ), namely, the excitation efficiency curve E(λ).

4. The method for calculating the intensity of a digital phantom using illumination light feedback according to claim 1, characterized in that: The process of obtaining the fluorescence emission intensity I(c) at any point in space includes: Under the conditions of the first excitation power P1 and the first excitation wavelength A(λ) of the fluorescence collection system, the fluorescence emission intensity of a series of fluorescent molecule samples with a concentration gradient is detected, and the fluorescent molecule concentration and the fluorescence emission intensity are fitted to obtain the fluorescence emission intensity I(c) at any point in space.

5. The method for calculating the intensity of a digital phantom using illumination light feedback according to claim 1, characterized in that: The process of calculating the fluorescence emission ratio η(c) based on the second excitation wavelength B(λ) of the device under test, the excitation efficiency curve E(λ), the first excitation wavelength A(λ) of the fluorescence collection system, the second excitation power P2 of the device under test, and the first excitation power P1 of the fluorescence collection system includes: Calculate the integral of the first excitation wavelength A(λ) of the fluorescence collection system and the excitation efficiency curve E(λ) to obtain the relative fluorescence excitation amount corresponding to the fluorescence collection system; Calculate the integral of the second excitation wavelength B(λ) of the device under test and the excitation efficiency curve E(λ) to obtain the relative fluorescence excitation amount corresponding to the device under test; The ratio of the relative fluorescence excitation amount corresponding to the fluorescence collection system to the relative fluorescence excitation amount corresponding to the device to be tested is taken as the relative fluorescence emission ratio; The ratio of the second excitation power P2 of the device under test to the first excitation power P1 of the fluorescence collection system is calculated, and the ratio is multiplied by the relative fluorescence emission ratio to obtain the absolute fluorescence emission ratio η(c).

6. The method for calculating the intensity of a digital phantom using illumination light feedback according to claim 1, characterized in that: The method further comprises: According to the fluorescence emission intensity I(c) at any point in space, the fluorescence emission intensity in two-dimensional space is obtained and recorded as I1(c,x,y); Obtain a first laser two-dimensional plane distribution D1 (x, y) of the fluorescence collection system, and obtain a second laser two-dimensional plane distribution D2 (x, y) of the device to be tested; The two-dimensional plane fluorescence emission intensity change is obtained according to the first laser two-dimensional plane distribution D1 (x, y) and the second laser two-dimensional plane distribution D2 (x, y) Thus, the digital phantom strength I2(c,x,y)=η(c,x,t)I1(c,x,y) is obtained.

7. A method for calculating the intensity of a digital phantom using illumination light feedback according to claim 1 or 6, characterized in that: The process of obtaining the first excitation power P1 and the first excitation wavelength A(λ) of the fluorescence collection system includes: A black and white camera and a lens group are placed and assembled, and within the imaging distance range, a first laser two-dimensional plane distribution of the illumination light is detected by a two-dimensional plane detector; At a fixed central position on the imaging surface, a first excitation power P1 of the illumination light is recorded by an optical power meter; At a fixed central position of the imaging surface, the first excitation spectrum of the illumination light is recorded by a spectrometer fiber probe, thereby obtaining a first excitation wavelength A(λ); The process of obtaining the second excitation power P2 and the second excitation wavelength B(λ) of the device under test includes: A black and white camera and a lens group are placed and assembled, and within the imaging distance range, a two-dimensional plane distribution of a second laser of the illumination light is detected by a two-dimensional plane detector; At a fixed central position on the imaging surface, a second excitation power P1 of the illumination light is recorded by an optical power meter; At a fixed central position of the imaging plane, the second excitation spectrum of the illumination light is recorded by the optical fiber probe of the spectrometer, thereby obtaining the second excitation wavelength A(λ).

8. An electronic device, comprising a memory and a processor, characterized in that: The memory is coupled to the processor; wherein the memory is used to store program data, and the processor is used to execute the program data to implement the digital phantom intensity calculation method using illumination light feedback as described in any one of claims 1 to 7.

9. A computer-readable storage medium having a computer program stored thereon, characterized in that: When the program is executed by a processor, the method for calculating the intensity of a digital phantom using illumination light feedback as claimed in any one of claims 1 to 7 is implemented.

10. A computer program product comprising a computer program / instructions, characterized in that When the computer program / instruction is executed by a processor, the method for calculating the intensity of a digital phantom using illumination light feedback as described in any one of claims 1 to 7 is implemented.

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