Composition for preventing osteoporosis

Abstract

The invention relates to the field of health food, in particular to a composition for preventing osteoporosis. The invention discloses a composition for preventing osteoporosis. Comprising the following components in parts by weight: 30-40 parts of glucosamine sulfate, 13-40 parts of calcium carbonate, 15-20 parts of sodium chondroitin sulfate and 10-30 parts of collagen, and the collagen comprises type I collagen and type II collagen. Various kinds of collagen are screened and added into the composition, and after the collagen type I and the collagen type II are added at the same time, the composition can remarkably promote bone cell growth and enhance calcium absorption, so that the composition is beneficial to increase of bone mineral density and prevention of osteoporosis. Meanwhile, the sturgeon cartilage is screened and used as the II-type collagen source, so that the effect is more remarkable.

Description

Technical Field

[0001] The invention relates to the field of health care products and foods, in particular to a composition for preventing osteoporosis. Background Art

[0002] Osteoporosis is one of the most common bone diseases in clinical practice. It is a metabolic bone disease characterized by low bone mass and degeneration and destruction of bone tissue microstructure, which leads to increased bone brittleness, decreased bone density, and easy fractures. Osteoporosis is more common in postmenopausal women. In addition, the risk of osteoarthritis in osteoporosis patients is several times that of the normal population.

[0003] At present, products that increase bone density are a major category of health foods. Raw materials that increase bone density are used in health products, and their effective ingredients include Chinese herbal medicine, chondroitin sulfate, glucosamine, collagen, vitamins, etc. Through the synergistic effect of added collagen, glucosamine sulfate, calcium carbonate, and sodium chondroitin sulfate, users can increase bone density and reduce the incidence of osteoporosis. However, due to the rich content and wide distribution of collagen, there are more than 28 known types of collagen, which are named type I, type II, type III, etc.

[0004] For example, CN 116210911 B discloses a shark chondroitin sulfate high calcium tablet and its preparation method, which only discloses the addition of collagen, but the specific type of collagen is not disclosed. Different types of collagen have different effects on the human body. In health food, what kind of collagen component can be added to effectively increase bone density, which is an urgent problem to be solved. Summary of the invention

[0005] The present invention aims at a composition for preventing osteoporosis, which is used in health food.

[0006] To achieve the purpose of the present invention, the technical solution is as follows:

[0007] A composition for preventing osteoporosis; characterized in that the composition comprises the following components in parts by weight: 30-40 parts of glucosamine sulfate, 13-40 parts of calcium carbonate, 15-20 parts of sodium chondroitin sulfate, and 10-30 parts of collagen.

[0008] Preferably, the composition comprises the following components in parts by weight: 38 parts of glucosamine sulfate, 25 parts of calcium carbonate, 20 parts of sodium chondroitin sulfate, and 20 parts of collagen.

[0009] More preferably, the collagen includes type I collagen and type II collagen, and the weight ratio of type I collagen to type II collagen is (4:6) to (6:4).

[0010] Furthermore, the type I collagen is derived from fish tissue and the type II collagen is derived from fish cartilage. Furthermore, the type I collagen is derived from tilapia skin and the type II collagen is derived from sturgeon cartilage.

[0011] Furthermore, the type I collagen is derived from tilapia skin, and its preparation method comprises the following steps:

[0012] S1 takes fish skin, slices it, rinses it, and cuts it into pieces;

[0013] S2: soaking the fish skin with NaCl solution, and filtering out the solution;

[0014] After S3 was soaked with SDS solution, the solution was filtered off;

[0015] S4 was added to the alcohol solution and stirred for 12 h, and the solution was filtered off;

[0016] S5 added NaOH solution, stirred at a high speed for 12 h, and filtered the solution;

[0017] S6 added acetic acid solution and stirred for 48h;

[0018] S7 added pepsin and stirred the reaction for 48 h;

[0019] S8 centrifuge, take the supernatant and filter it through a filter membrane and an ultrafiltration membrane;

[0020] S9 freeze-dried.

[0021] In the above method, in step S2, the mass concentration of NaCl solution is 8%. The amount of SDS solution is 30L, and the mass concentration is 0.6%. In step S4, the alcohol solution is selected from n-butanol, and the mass concentration is 5%. In step S5, the NaOH solution is 30L of 0.2M NaOH solution. In step S6, the acetic acid solution is 1M, 100L. In step S8, the centrifugal speed is 15000rpm, and the centrifugal time is 20min. In step S8, the pressure of the supernatant filtered through the filter membrane and ultrafiltration membrane is 0.40-0.50Mpa. The freeze-drying time of step S9 is 72h.

[0022] The type II collagen is derived from sturgeon cartilage, and its preparation method comprises the following steps:

[0023] S1 Rinse the sturgeon cartilage, cut into pieces, and freeze and mince;

[0024] S2 is steamed at 110-120℃ for 20-50 min;

[0025] S3 plus pancreatin, enzymatic hydrolysis;

[0026] S4: taking the above enzymatic hydrolysate, centrifuging and obtaining a supernatant;

[0027] S5 enzyme inactivation;

[0028] S6 was salted out by adding NaCl powder and left to stand overnight;

[0029] S7 centrifuges the salting liquid, takes the supernatant after centrifugation, and sterilizes it;

[0030] S8 freeze drying.

[0031] In the above method, the cooking temperature in step S2 is preferably 120°C for 20-40 minutes. More preferably, the cooking temperature is preferably 120°C for 30 minutes. Using a higher cooking temperature helps to improve the effect of pyrolysis of the raw material and facilitates the effect of the subsequent enzymatic hydrolysis. As the cooking time increases, the extraction rate of collagen increases and tends to be stable after 30 minutes.

[0032] In the above method, in step S4, the amount of pancreatic enzyme added was 8000 U / g·Pro, the enzymatic hydrolysis time was 4 h, the enzymatic hydrolysis pH was 8, and the enzymatic hydrolysis temperature was 50°C.

[0033] In the above method, in step S4, the centrifugal speed is 10000 r / min, and the centrifugal time is 30 min. In step S5, the enzyme is inactivated by adjusting the pH value of the solution to 7, and an acid solution can be used. In step S7, the centrifugal speed is 10000 r / min, and the centrifugal time is 30 min.

[0034] The composition for preventing osteoporosis comprises mixing the components according to a proportion.

[0035] Compared with the prior art, the present invention has the following significant advantages:

[0036] The present invention screens various collagens and adds them to the composition. It is found that when type I and type II collagen are added at the same time, the composition can significantly promote the growth of bone cells and enhance the effect of calcium absorption, thereby being beneficial to the increase of bone density and the prevention of osteoporosis. At the same time, by screening and using sturgeon cartilage as a source of type II collagen, the effect is more significant. This may be because the type II collagen of sturgeon is more conducive to the absorption and utilization of the human body. Sturgeon cartilage accounts for about 5.7% of the sturgeon body. Except for the partially ossified fins and true bones of the head, the rest of the bones are cartilage. Therefore, using sturgeon cartilage as a source of extracting type II collagen and adding it to health foods provides the possibility of large-scale industrial production. DETAILED DESCRIPTION

[0037] Example 1 Preparation of Type I Collagen

[0038] Preparation of type I collagen, extracted from tilapia skin, the specific production method is as follows:

[0039] S1, weigh 2500g tilapia fish skin, cut it into thin slices with a blade, use pure water to rinse the shredded fish skin 3 times, clean the fish skin surface stains and other impurities among the fish skin;

[0040] S2, with mass concentration be 8% NaCl solution 30L with fish skin soaked 4h after, filtering solution;

[0041] S3, soak in 30L of 0.6% SDS solution for 3h, filter out the solution;

[0042] S4, after washing three times with pure water, add 30L of 5% n-butanol solution, stir at 60rpm for 12h, and filter out the solution;

[0043] S5. Add 30 L of 0.2 M NaOH solution into the reactor, stir at the same speed for 12 h, and filter out the solution;

[0044] S6. After washing three times with pure water, add 100 L of 1M acetic acid solution and stir for 48 h;

[0045] S7, add 1 kg of pepsin and stir to react for 48 hours;

[0046] S8. Transfer the liquid after the reaction into a centrifuge, centrifuge at 15000 rpm for 20 min, take the supernatant and remove the precipitate;

[0047] S9. Under a pressure of 0.45 MPa, the supernatant liquid is sequentially passed through a large-pore filter membrane, a small-pore filter membrane and an ultrafiltration membrane to remove large particles, microorganisms, small molecules, salt and other impurities, and finally a sterile collagen solution with a molecular weight of ≥100 Kb is obtained;

[0048] S10, placing in a freeze dryer, freeze drying for 72 hours to obtain dry type I collagen powder.

[0049] Example 2 Preparation of Type II Collagen

[0050] Preparation of type II collagen, extracted from sturgeon cartilage, the specific production method is as follows:

[0051] S1. Pre-treat the sturgeon cartilage: rinse the sturgeon cartilage with ice water to remove impurities and non-cartilage tissue on its surface, cut it into pieces, and then freeze and mince it;

[0052] S2, steaming at 120°C for 30 min;

[0053] S3, add pancreatic enzyme at a dosage of 8000 U / g·Pro, enzymatic hydrolysis time of 4 h, enzymatic hydrolysis pH value of 8, and enzymatic hydrolysis temperature of 50°C;

[0054] S4, centrifuge the enzymatic hydrolysate at 10000 r / min for 30 min, and take the supernatant;

[0055] S5, pH to 7, enzyme inactivation;

[0056] S6. Slowly add NaCl powder for salting out, and keep stirring. After all the NaCl is dissolved, let it stand overnight.

[0057] S7, centrifuge the salting liquid at 4°C, 10000r / min, for 30min, and sterilize the supernatant after centrifugation;

[0058] S8. Freeze-dry for 48 hours to obtain sturgeon cartilage type II collagen.

[0059] Example 3 Preparation of Composition 1 for Preventing Osteoporosis

[0060] Take 38 parts of glucosamine sulfate, 25 parts of calcium carbonate, 20 parts of sodium chondroitin sulfate, and 20 parts of collagen, wherein the collagen is 10 parts of type I collagen in Example 1 and 10 parts of type II collagen in Example 2, and mix them evenly to form a powder.

[0061] Example 4 Preparation of Composition 2 for Preventing Osteoporosis

[0062] Take 38 parts of glucosamine sulfate, 25 parts of calcium carbonate, 20 parts of sodium chondroitin sulfate, and 20 parts of collagen, wherein the collagen is 8 parts of type I collagen in Example 1 and 12 parts of type II collagen in Example 2, and mix them evenly to form a powder.

[0063] Example 5 Preparation of Composition 3 for Preventing Osteoporosis

[0064] Take 38 parts of glucosamine sulfate, 25 parts of calcium carbonate, 20 parts of sodium chondroitin sulfate, and 20 parts of collagen, wherein the collagen is 12 parts of type I collagen in Example 1 and 8 parts of type II collagen in Example 2, and mix them evenly to form a powder.

[0065] Comparative Example 1

[0066] Take 38 parts of glucosamine sulfate, 25 parts of calcium carbonate, 20 parts of sodium chondroitin sulfate, and 20 parts of collagen, wherein the collagen is 20 parts of type I collagen in Example 1, and mix them evenly to form a powder.

[0067] Comparative Example 2

[0068] Take 38 parts of glucosamine sulfate, 25 parts of calcium carbonate, 20 parts of sodium chondroitin sulfate, and 20 parts of collagen, wherein the collagen is 10 parts of type I collagen in Example 1 and 10 parts of type II collagen of chicken cartilage purchased from commercial channels, and mix them evenly to form a powder.

[0069] Comparative Example 3

[0070] Take 38 parts of glucosamine sulfate, 25 parts of calcium carbonate, 20 parts of sodium chondroitin sulfate, and 20 parts of collagen, wherein the collagen is 10 parts of type I collagen in Example 1 and 10 parts of type II collagen from bovine cartilage purchased from commercial channels, and mix them evenly to form a powder.

[0071] Example 7 Effect Verification

[0072] 1. Experimental animals and feeding conditions: 60 SPF Wistar rats, male, body weight: 100-105 g, feeding temperature of 21°C to 23°C, feeding humidity of 55%RH to 58%RH.

[0073] 2. Administration route of test animals: Rats were gavaged 3 times a day, 50 mg / kg each time. After 7 days of adaptive feeding, the rats were randomly divided into 6 groups, 10 rats in each group, A / B / C / D / E / F, and kept in separate cages. They drank deionized water to avoid obtaining calcium from drinking water. After 3 months of feeding, various indicators of bone density increase were tested, and the results are shown in Table 1 below.

[0074] Table 1

[0075]

[0076] The results of Table 1 show that after 3 months of feeding, the femur weight of rats in group B was 0.56±0.03g, while that of group D was 0.35±0.04g, the heaviest femur weight of rats in group AC was 0.56±0.03g, and the lightest femur weight of rats in group DF was 0.35±0.04g. The femur weight of rats in group AC was much greater than that in group DF. The bone calcium content was 198±6.2g in group B, 168±5.4g in group D, and 198±6.2g in group AC. The test results of bone calcium content also showed that the bone calcium content of rats in group AC was significantly higher than that in group DF. The results of midpoint bone density and distal end bone density also show that the composition provided by the present invention is beneficial for preventing osteoporosis and has a significant effect.

[0077] The innovation of the present invention is that the present invention can promote bone cell growth and enhance calcium absorption by adding type II collagen, especially sturgeon cartilage type II collagen, so as to be beneficial to increase bone density and prevent osteoporosis.

[0078] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be considered exemplary and non-restrictive in all respects, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention.

[0079] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A composition for preventing osteoporosis, characterized in that: The composition comprises the following components in parts by weight: 30-40 parts of glucosamine sulfate, 13-40 parts of calcium carbonate, 15-20 parts of sodium chondroitin sulfate, and 10-30 parts of collagen; the collagen comprises type I collagen and type II collagen.

2. The composition for preventing osteoporosis according to claim 1, characterized in that: The composition comprises the following components in parts by weight: 38 parts of glucosamine sulfate, 25 parts of calcium carbonate, 20 parts of sodium chondroitin sulfate, and 20 parts of collagen.

3. The composition for preventing osteoporosis according to claim 2, characterized in that: The weight ratio of type I collagen to type II collagen is (4:6) to (6:4).

4. The composition for preventing osteoporosis according to claim 3, characterized in that: The type I collagen and type II collagen are both derived from fish tissues.

5. The composition for preventing osteoporosis according to claim 4, characterized in that: The type I collagen is derived from fish skin and the type II collagen is derived from fish cartilage.

6. The composition for preventing osteoporosis according to claim 5, characterized in that: The type I collagen is derived from tilapia skin; the type II collagen is derived from sturgeon cartilage.

7. The composition for preventing osteoporosis according to claim 6, characterized in that: The method for preparing type I collagen comprises the following steps: S1 takes fish skin, slices it, rinses it, and cuts it into pieces; S2: soaking the fish skin with NaCl solution, and filtering out the solution; After S3 was soaked with SDS solution, the solution was filtered off; S4 was added to the alcohol solution and stirred for 12 h, and the solution was filtered off; S5 added NaOH solution, stirred at a high speed for 12 h, and filtered the solution; S6 added acetic acid solution and stirred for 48h; S7 added pepsin and stirred the reaction for 48 h; S8 centrifuge, take the supernatant and filter it through a filter membrane and an ultrafiltration membrane; S9 freeze-dried.

8. The composition for preventing osteoporosis according to claim 6, characterized in that: The method for preparing type II collagen comprises the following steps: S1 Rinse the sturgeon cartilage, cut into pieces, and freeze and mince; S2 is steamed at 110-120℃ for 20-50 min; S3 plus pancreatin, enzymatic hydrolysis; S4: taking the above enzymatic hydrolysate, centrifuging and obtaining a supernatant; S5 enzyme inactivation; S6 was salted out by adding NaCl powder and left to stand overnight; S7 centrifuges the salting liquid, takes the supernatant after centrifugation, and sterilizes it; S8 freeze drying.

9. The composition for preventing osteoporosis according to claim 1, characterized in that: Including mixing the components according to the proportions.

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