Gel containing polynucleotide sodium hyaluronate and preparation method thereof

Abstract

The invention relates to the technical field of medicines, and particularly discloses gel containing polynucleotide sodium hyaluronate and a preparation method of the gel. The invention discloses gel containing polynucleotide and sodium hyaluronate. The gel is prepared from the following raw material components in parts by weight: 45 to 60 parts of mannitol, 8 to 12 parts of polynucleotide, 8 to 12 parts of sodium hyaluronate, 0.5 to 1.5 parts of a humectant, 0.5 to 1.5 parts of a whitening agent and 0.5 to 1 part of a stabilizer. The mannitol, the polynucleotide and the sodium hyaluronate are matched, so that the skin elasticity can be improved, and the skin can be tightened; and the moisturizing agent and the whitening agent are added, so that the moisturizing and whitening effects of the prepared gel can be improved.

Description

Technical Field

[0001] The present application relates to the technical field of medicine, and in particular to a polynucleotide sodium hyaluronate gel and a preparation method thereof. Background Art

[0002] Hyaluronic acid products are currently the most popular absorbable skin fillers. As a physiologically active substance, they are widely distributed in the extracellular matrix of connective tissues of animals and humans. Its irregular bending state in the solution and its fluid dynamics properties give it some important physical properties such as high viscoelasticity, plasticity, unique rheological properties and good biocompatibility. Because of this, it is a widely used absorbable biomaterial.

[0003] Mannitol is a common pharmaceutical excipient and is widely used in the medical field. As a free radical scavenger, mannitol can effectively limit the oxidative damage of free radicals to the skin, prevent free radicals from degrading the product, thereby reducing the redness and swelling reaction after injection, slowing down the degradation of gel molecules, and making the therapeutic effect more lasting. At the same time, it can be used as an osmotic pressure regulator to maintain isotonic conditions similar to those in the body.

[0004] Polynucleotide (PN) is a natural macromolecular deoxyribonucleic acid polymer extracted from salmon testes (semen) through a rigorous purification process, with high safety and stability. Its mechanism of action is to provide support for the skin through a 3D specific structure; the three-dimensional structure can serve as a weak support body during the growth of cells and extracellular matrix (ECM), and can regenerate tissues; it is a structure suitable for fibroblast regeneration; it is biodegradable and has a neutral pH value. These advantages can enhance the nutrition of dermal cells to improve the fullness of the skin, increase skin elasticity and tighten the skin, making the skin healthier, higher quality and more natural. Studies have shown that when polydeoxyribonucleotides and sodium hyaluronate are used in combination, the effect of promoting wound healing is significantly higher than that of sodium hyaluronate alone, showing the mutual promotion effect of the two, but the moisturizing and whitening effect on the skin needs to be improved. Summary of the invention

[0005] In order to improve the moisturizing and whitening effects of skin fillers, the present application provides a gel containing polynucleotide sodium hyaluronate and a preparation method thereof.

[0006] In the first aspect, the present application provides a polynucleotide sodium hyaluronate gel, which adopts the following technical solution: A polynucleotide-containing sodium hyaluronate gel, wherein the raw materials of the gel include the following components in parts by weight: 45-60 parts of mannitol, 8-12 parts of polynucleotide, 8-12 parts of sodium hyaluronate, 0.5-1.5 parts of moisturizer, 0.5-1.5 parts of whitening agent, and 0.5-1 part of stabilizer.

[0007] By adopting the above technical scheme, the combination of mannitol, polynucleotide and sodium hyaluronate can increase skin elasticity and tighten the skin; and the addition of moisturizers and whitening agents can improve the moisturizing and whitening effects of the prepared gel.

[0008] In a specific embodiment, the moisturizing agent includes a mixture of aloe vera extract and glycerin.

[0009] By adopting the above technical solution and utilizing the composite moisturizer composed of aloe extract and glycerin, the moisturizing effect of the gel can be improved.

[0010] In a specific embodiment, the preparation method of the aloe extract comprises the following steps: Pretreatment: first wash the aloe leaves, then slice and crush them to obtain aloe pieces; Extraction: extracting aloe vera powder with an extractant to obtain an extract; Filtration: Filter the extract to obtain a filtrate; Freeze drying: The filtrate is freeze dried to obtain aloe vera extract.

[0011] By adopting the above technical scheme, the aloe leaves are first sliced ​​and crushed to obtain aloe crushed materials, then extracted with an extractant, filtered, and finally the obtained filtrate is freeze-dried to obtain the aloe extract.

[0012] In a specific embodiment, the freeze-drying step is: first freezing the filtrate at -20--10°C, and then heating and drying at 0.01MPa-0.1MPa vacuum and 20-40°C to obtain the aloe extract.

[0013] By adopting the above technical solution, the freeze-drying conditions are further limited, thereby improving the quality of the obtained aloe extract.

[0014] In a specific embodiment, the whitening agent includes magnesium L-ascorbyl phosphate.

[0015] By adopting the above technical solution, L-ascorbyl magnesium phosphate can not only improve the whitening effect of the gel, but also improve the stability and antioxidant performance of the gel.

[0016] In a specific embodiment, the stabilizer includes a mixture of carbomer and vitamin E.

[0017] By adopting the above technical solution and using a composite stabilizer composed of carbomer and vitamin E, the stability of the gel can be further improved.

[0018] In a second aspect, the present application provides a method for preparing a polynucleotide sodium hyaluronate gel, which adopts the following technical scheme: A method for preparing a polynucleotide sodium hyaluronate gel comprises the following steps: First, add mannitol to the buffer solution, stir until completely dissolved, filter using an aqueous filter membrane, and sterilize the filtrate at 121°C for 30 minutes to obtain a mixed solution; Add polynucleotide, sodium hyaluronate powder, moisturizer, whitening agent, stabilizer to the mixed solution, shake it slowly, centrifuge it, and finally sterilize it with wet heat to obtain a gel containing polynucleotide sodium hyaluronate.

[0019] By adopting the above technical scheme, mannitol is first added to the buffer solution to dissolve it, then filtered and sterilized at high temperature to obtain a mixed solution, and finally polynucleotides, sodium hyaluronate dry powder, moisturizers, whitening agents, and stabilizers are added to the mixed solution, stirred and mixed evenly, centrifuged, and sterilized with wet heat to obtain a polynucleotide sodium hyaluronate gel with good moisturizing and whitening effects.

[0020] In a specific embodiment, the buffer is a mixture of disodium hydrogen phosphate dodecahydrate, sodium dihydrogen phosphate dihydrate, sodium chloride, and water for injection.

[0021] In summary, the present application includes at least one of the following beneficial technical effects: In the present application, the combination of mannitol, polynucleotides and sodium hyaluronate can increase skin elasticity and tighten the skin; and the addition of moisturizers and whitening agents can improve the moisturizing and whitening effects of the prepared gel; In the present application, aloe leaves are first sliced ​​and crushed to obtain aloe fragments, then extracted with an extractant, filtered, and finally the obtained filtrate is freeze-dried to obtain aloe extract; The method in the present application first adds mannitol to a buffer solution to dissolve it, then filters it, and sterilizes it at high temperature to obtain a mixed solution. Finally, polynucleotides, sodium hyaluronate dry powder, moisturizers, whitening agents, and stabilizers are added to the mixed solution, stirred and mixed evenly, centrifuged, and sterilized with wet heat to obtain a polynucleotide sodium hyaluronate gel with good moisturizing and whitening effects. DETAILED DESCRIPTION

[0022] The present application is further described in detail below with reference to the embodiments.

[0023] All raw materials in the examples are commercially available.

[0024] Preparation Example 1 Preparation Example 1 provides a method for preparing an aloe extract, comprising the following steps: Pretreatment: Select fresh aloe leaves with a growth period of more than 6 months, wash the aloe leaves, slice them into slices with a thickness of 2-3 mm, and crush them to obtain aloe fragments; Extraction: extracting aloe vera powder with an extractant at 50°C to obtain an extract; wherein the extractant is an ethanol solution with a mass concentration of 65%; Filtration: Filter the extract using a 100-mesh filter to obtain a filtrate; Freeze drying: The filtrate was first frozen at -20°C, and then heated and dried at 20°C under a vacuum degree of 0.01 MPa to obtain the aloe extract.

[0025] Preparation Example 2 Preparation Example 2 provides a method for preparing an aloe extract, comprising the following steps: Pretreatment: Select fresh aloe leaves with a growth period of more than 6 months, wash the aloe leaves, slice them into slices with a thickness of 2-3 mm, and crush them to obtain aloe fragments; Extraction: extracting aloe vera powder with an extractant at 50°C to obtain an extract; wherein the extractant is an ethanol solution with a mass concentration of 65%; Filtration: Filter the extract using a 100-mesh filter to obtain a filtrate; Freeze drying: The filtrate was first frozen at -15°C, and then heated and dried at 30°C under a vacuum degree of 0.05 MPa to obtain the aloe extract.

[0026] Preparation Example 3 Preparation Example 3 provides a method for preparing an aloe extract, comprising the following steps: Pretreatment: Select fresh aloe leaves with a growth period of more than 6 months, wash the aloe leaves, slice them into slices with a thickness of 2-3 mm, and crush them to obtain aloe fragments; Extraction: extracting aloe vera powder with an extractant at 50°C to obtain an extract; wherein the extractant is an ethanol solution with a mass concentration of 65%; Filtration: Filter the extract using a 100-mesh filter to obtain a filtrate; Freeze drying: The filtrate was first frozen at -10°C, and then heated and dried at 40°C under a vacuum degree of 0.1 MPa to obtain the aloe extract. Example

[0027] Example 1 provides a method for preparing a polynucleotide sodium hyaluronate gel, comprising the following steps: First, add 45g of mannitol to 1L of buffer solution, stir until completely dissolved, filter with a 0.22um aqueous filter membrane, and sterilize the filtrate at 121°C for 30 minutes to obtain a mixed solution; wherein the buffer solution is a mixed solution composed of disodium hydrogen phosphate dodecahydrate, sodium dihydrogen phosphate dihydrate, sodium chloride, and water for injection, and the pH value is 7.0; Under a laminar flow hood, add 8 g of polynucleotide, 8 g of sodium hyaluronate powder, 0.5 g of moisturizer, 0.5 g of whitening agent, and 0.5 g of stabilizer to the mixed solution, shake it slowly, fill it into a prefilled syringe, add a stopper in time, centrifuge it after stoppering, and finally sterilize it with wet heat at 121°C for 15 minutes to obtain a gel containing polynucleotide sodium hyaluronate; wherein the moisturizer is a mixture of aloe extract and glycerol in Preparation 1, and the weight ratio of aloe extract to glycerol is 2:1; the whitening agent is L-ascorbyl magnesium phosphate; the stabilizer is a mixture of carbomer and vitamin E, and the weight ratio of carbomer to vitamin E is 1:1. Example 2 provides a method for preparing a polynucleotide sodium hyaluronate gel, comprising the following steps:

[0028] First, add 45g of mannitol to 1L of buffer solution, stir until completely dissolved, filter with a 0.22um aqueous filter membrane, and sterilize the filtrate at 121°C for 30 minutes to obtain a mixed solution; wherein the buffer solution is a mixed solution composed of disodium hydrogen phosphate dodecahydrate, sodium dihydrogen phosphate dihydrate, sodium chloride, and water for injection, and the pH value is 7.0; Under a laminar flow hood, add 8 g of polynucleotide, 8 g of sodium hyaluronate powder, 0.5 g of moisturizer, 0.5 g of whitening agent, and 0.5 g of stabilizer to the mixed solution, shake it slowly, fill it into a prefilled syringe, add a stopper in time, centrifuge it after stoppering, and finally sterilize it with wet heat at 121°C for 15 minutes to obtain a gel containing polynucleotide sodium hyaluronate; wherein the moisturizer is a mixture of aloe extract and glycerol in Preparation 2, and the weight ratio of aloe extract to glycerol is 2:1; the whitening agent is L-ascorbyl magnesium phosphate; the stabilizer is a mixture of carbomer and vitamin E, and the weight ratio of carbomer to vitamin E is 1:1. Example 3 provides a method for preparing a polynucleotide sodium hyaluronate gel, comprising the following steps:

[0029] First, add 45g of mannitol to 1L of buffer solution, stir until completely dissolved, filter with a 0.22um aqueous filter membrane, and sterilize the filtrate at 121°C for 30 minutes to obtain a mixed solution; wherein the buffer solution is a mixed solution composed of disodium hydrogen phosphate dodecahydrate, sodium dihydrogen phosphate dihydrate, sodium chloride, and water for injection, and the pH value is 7.0; Under a laminar flow hood, add 8 g of polynucleotide, 8 g of sodium hyaluronate powder, 0.5 g of moisturizer, 0.5 g of whitening agent, and 0.5 g of stabilizer to the mixed solution, shake it slowly, fill it into a prefilled syringe, add a stopper in time, centrifuge it after stoppering, and finally sterilize it with wet heat at 121°C for 15 minutes to obtain a gel containing polynucleotide sodium hyaluronate; wherein the moisturizer is a mixture of aloe extract and glycerol in Preparation 3, and the weight ratio of aloe extract to glycerol is 2:1; the whitening agent is L-ascorbyl magnesium phosphate; the stabilizer is a mixture of carbomer and vitamin E, and the weight ratio of carbomer to vitamin E is 1:1. Example 4 provides a method for preparing a polynucleotide sodium hyaluronate gel, comprising the following steps:

[0030] First, add 52.5g of mannitol to 1L of buffer solution, stir until completely dissolved, filter with a 0.22um aqueous filter membrane, and sterilize the filtrate at 121°C for 30 minutes to obtain a mixed solution; wherein the buffer solution is a mixed solution composed of disodium hydrogen phosphate dodecahydrate, sodium dihydrogen phosphate dihydrate, sodium chloride, and water for injection, and the pH value is 7.0; Under a laminar flow hood, add 10 g of polynucleotide, 10 g of sodium hyaluronate powder, 1 g of moisturizer, 1 g of whitening agent, and 0.8 g of stabilizer to the mixed solution, shake it slowly, fill it into a prefilled syringe, add a stopper in time, centrifuge it after stoppering, and finally sterilize it with wet heat at 121°C for 15 minutes to obtain a gel containing polynucleotide sodium hyaluronate; wherein the moisturizer is a mixture of aloe extract and glycerol in Preparation 2, and the weight ratio of aloe extract to glycerol is 2:1; the whitening agent is L-ascorbyl magnesium phosphate; the stabilizer is a mixture of carbomer and vitamin E, and the weight ratio of carbomer to vitamin E is 1:1. Example 5 provides a method for preparing a polynucleotide sodium hyaluronate gel, comprising the following steps:

[0031] First, add 60g of mannitol to 1L of buffer solution, stir until completely dissolved, filter with a 0.22um aqueous filter membrane, and sterilize the filtrate at 121°C for 30 minutes to obtain a mixed solution; wherein the buffer solution is a mixed solution composed of disodium hydrogen phosphate dodecahydrate, sodium dihydrogen phosphate dihydrate, sodium chloride, and water for injection, and the pH value is 7.0; Under a laminar flow hood, add 12 g of polynucleotide, 12 g of sodium hyaluronate powder, 1.5 g of moisturizer, 1.5 g of whitening agent, and 1 g of stabilizer to the mixed solution, shake it slowly, fill it into a prefilled syringe, add a stopper in time, centrifuge it after stoppering, and finally sterilize it with wet heat at 121°C for 15 min to obtain a gel containing polynucleotide sodium hyaluronate; wherein the moisturizer is a mixture of aloe extract and glycerol in Preparation 2, and the weight ratio of aloe extract to glycerol is 2:1; the whitening agent is L-ascorbyl magnesium phosphate; the stabilizer is a mixture of carbomer and vitamin E, and the weight ratio of carbomer to vitamin E is 1:1. Example 6 provides a method for preparing a gel containing polynucleotide sodium hyaluronate, comprising the following steps:

[0032] First, add 52.5g of mannitol to 1L of buffer solution, stir until completely dissolved, filter with a 0.22um aqueous filter membrane, and sterilize the filtrate at 121°C for 30 minutes to obtain a mixed solution; wherein the buffer solution is a mixed solution composed of disodium hydrogen phosphate dodecahydrate, sodium dihydrogen phosphate dihydrate, sodium chloride, and water for injection, and the pH value is 7.0; Under a laminar flow hood, add 10 g of polynucleotide, 10 g of sodium hyaluronate powder, 1 g of moisturizer, 1 g of whitening agent, and 0.8 g of stabilizer to the mixed solution, shake it slowly, fill it into a prefilled syringe, add a stopper in time, centrifuge it after stoppering, and finally sterilize it with wet heat at 121°C for 15 min to obtain a gel containing polynucleotide sodium hyaluronate; wherein the moisturizer is the aloe extract in Preparation 2; the whitening agent is L-ascorbyl magnesium phosphate; the stabilizer is a mixture of carbomer and vitamin E, and the weight ratio of carbomer to vitamin E is 1:1. Example 7 provides a method for preparing a gel containing polynucleotide sodium hyaluronate, comprising the following steps:

[0033] First, add 52.5g of mannitol to 1L of buffer solution, stir until completely dissolved, filter with a 0.22um aqueous filter membrane, and sterilize the filtrate at 121°C for 30 minutes to obtain a mixed solution; wherein the buffer solution is a mixed solution composed of disodium hydrogen phosphate dodecahydrate, sodium dihydrogen phosphate dihydrate, sodium chloride, and water for injection, and the pH value is 7.0; Under a laminar flow hood, add 10 g of polynucleotide, 10 g of sodium hyaluronate powder, 1 g of moisturizer, 1 g of whitening agent, and 0.8 g of stabilizer to the mixed solution, shake it slowly, fill it into a prefilled syringe, add a stopper in time, centrifuge it after stoppering, and finally sterilize it with wet heat at 121°C for 15 minutes to obtain a gel containing polynucleotide sodium hyaluronate; wherein the moisturizer is glycerol; the whitening agent is L-ascorbyl magnesium phosphate; the stabilizer is a mixture of carbomer and vitamin E, and the weight ratio of carbomer to vitamin E is 1:1. Example 8 provides a method for preparing a gel containing polynucleotide sodium hyaluronate, comprising the following steps:

[0034] First, add 52.5g of mannitol to 1L of buffer solution, stir until completely dissolved, filter with a 0.22um aqueous filter membrane, and sterilize the filtrate at 121°C for 30 minutes to obtain a mixed solution; wherein the buffer solution is a mixed solution composed of disodium hydrogen phosphate dodecahydrate, sodium dihydrogen phosphate dihydrate, sodium chloride, and water for injection, and the pH value is 7.0; Under a laminar flow hood, add 10 g of polynucleotide, 10 g of sodium hyaluronate powder, 1 g of moisturizer, 1 g of whitening agent, and 0.8 g of stabilizer to the mixed solution, shake it slowly, fill it into a prefilled syringe, add a stopper in time, centrifuge it after stoppering, and finally sterilize it with wet heat at 121°C for 15 min to obtain a gel containing polynucleotide sodium hyaluronate; wherein the moisturizer is a mixture of aloe extract and glycerol in Preparation 2, and the weight ratio of aloe extract to glycerol is 2:1; the whitening agent is L-ascorbyl magnesium phosphate; and the stabilizer is carbomer. Example 9 provides a method for preparing a polynucleotide sodium hyaluronate gel, comprising the following steps:

[0035] First, add 52.5g of mannitol to 1L of buffer solution, stir until completely dissolved, filter with a 0.22um aqueous filter membrane, and sterilize the filtrate at 121°C for 30 minutes to obtain a mixed solution; wherein the buffer solution is a mixed solution composed of disodium hydrogen phosphate dodecahydrate, sodium dihydrogen phosphate dihydrate, sodium chloride, and water for injection, and the pH value is 7.0; Under a laminar flow hood, add 10 g of polynucleotide, 10 g of sodium hyaluronate powder, 1 g of moisturizer, 1 g of whitening agent, and 0.8 g of stabilizer to the mixed solution, shake it slowly, fill it into a prefilled syringe, add a stopper in time, centrifuge it after stoppering, and finally sterilize it with wet heat at 121°C for 15 minutes to obtain a gel containing polynucleotide sodium hyaluronate; wherein the moisturizer is a mixture of aloe extract and glycerol in Preparation 2, and the weight ratio of aloe extract to glycerol is 2:1; the whitening agent is L-ascorbyl magnesium phosphate; and the stabilizer is vitamin E. Comparative Example 1

[0036] Comparative Example 1 provides a method for preparing a gel containing polynucleotide sodium hyaluronate, comprising the following steps: First, add 45g of mannitol to 1L of buffer solution, stir until completely dissolved, filter with a 0.22um aqueous filter membrane, and sterilize the filtrate at 121°C for 30 minutes to obtain a mixed solution; wherein the buffer solution is a mixed solution composed of disodium hydrogen phosphate dodecahydrate, sodium dihydrogen phosphate dihydrate, sodium chloride, and water for injection, and the pH value is 7.0; Under a laminar flow hood, add 8 g of polynucleotide and 8 g of sodium hyaluronate powder to the mixture, shake slowly, fill into a prefilled syringe, add a stopper in time, centrifuge after stoppering, and finally sterilize with wet heat at 121°C for 15 min to obtain a polynucleotide sodium hyaluronate gel.

[0037] Cosmetic effect: Twenty subjects who are willing and able to return for follow-up visits on time and receive follow-up visits in each group of embodiments and comparative examples are selected. They are in good health, hope to improve their facial skin quality, and are willing and able to provide accurate information. A 31G 9-needle medical hydro-light needle is used, and the Demasha hydro-light injector is used for full-face intradermal injection. The operating setting parameters of the Demasha hydro-light injector are: injection depth 1.0mm, negative pressure 3 gears, and unit injection volume 0.033mL each time. The needle is injected perpendicular to the face once. After the injection, a medical sodium hyaluronate mask is applied topically for 20 minutes. Two weeks after the injection, the subjects gave feedback. For the moisturizing and whitening effects, they were divided into three evaluation criteria: very satisfied, satisfied, and dissatisfied. Very satisfied means excellent effect, satisfied means effective, and dissatisfied means ineffective. People who choose each option are counted. For the optimal embodiment, ten subjects are selected again, and the injections are performed according to the same method as above. Then, the subjects are tested by the VISIA skin testing instrument four weeks later to obtain a VISIA facial scoring report, in which the percentile score describes the skin feature score compared with other people of the same age, gender, and skin type as the subject, and the higher the score, the better.

[0038] Table 1 Gel test results

[0039] Table 2 VISIA facial scoring report of the gel in Example 4

[0040] Table 3 VISIA facial scoring report of the gel in Example 4

[0041] Combining Table 1, Example 1 and Comparative Example 1, the moisturizing and whitening effect of the gel in Example 1 is better. It can be seen that when preparing the gel, adding a composite moisturizer composed of aloe extract and glycerin and a whitening agent of L-ascorbic acid magnesium phosphate to the raw materials can improve the moisturizing and beauty effect of the prepared gel.

[0042] Combining Table 1 and Examples 1-3, the moisturizing effect of the gel in Example 2 is the best. It can be seen that when preparing the aloe extract, the preparation conditions in Preparation Example 2 are the best, so that the moisturizing effect of the gel finally obtained is the best.

[0043] Combining Table 1, Example 2, Example 4 and Example 5, the moisturizing and whitening effect of the gel in Example 4 is the best. It can be seen that when preparing the gel, increasing the amount of raw materials used, the effect of the prepared gel shows a trend of first increasing and then decreasing.

[0044] Combining Table 1, Example 4, Example 6 and Example 7, the moisturizing effect of the gel in Example 4 is the best. It can be seen that when preparing the gel, the moisturizing agent is preferably a composite moisturizing agent composed of aloe extract and glycerin, and the moisturizing effect of the gel finally obtained is better.

[0045] Combining Table 1, Example 4, Example 8 and Example 9, the moisturizing and whitening effect of the gel in Example 4 is the best. It can be seen that when preparing the gel, the stabilizer is preferably a composite stabilizer composed of carbomer and vitamin E, which can further improve the effect of the gel.

[0046] Combining Table 2, Table 3 and Example 4, it can be seen that the gel in Example 4 has a good moisturizing and whitening effect.

[0047] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed, but such modifications are protected by the patent law as long as they are within the scope of the claims of the present application.

Claims

1. A polynucleotide sodium hyaluronate gel, characterized in that: The raw materials of the gel include the following components in parts by weight: 45-60 parts of mannitol, 8-12 parts of polynucleotides, 8-12 parts of sodium hyaluronate, 0.5-1.5 parts of moisturizers, 0.5-1.5 parts of whitening agents, and 0.5-1 parts of stabilizers.

2. The polynucleotide sodium hyaluronate gel according to claim 1, characterized in that: The moisturizing agent comprises a mixture of aloe vera extract and glycerin.

3. The polynucleotide sodium hyaluronate gel according to claim 2, characterized in that: The preparation method of the aloe extract comprises the following steps: Pretreatment: first wash the aloe leaves, then slice and crush them to obtain aloe pieces; Extraction: extracting aloe vera powder with an extractant to obtain an extract; Filtration: Filter the extract to obtain a filtrate; Freeze drying: The filtrate is freeze dried to obtain aloe vera extract.

4. The polynucleotide sodium hyaluronate gel according to claim 3, characterized in that: The freeze-drying step is: firstly freezing the filtrate at -20--10°C, and then heating and drying at 0.01MPa-0.1MPa vacuum degree and 20-40°C to obtain the aloe extract.

5. The polynucleotide sodium hyaluronate gel according to claim 1, characterized in that: The whitening agent includes magnesium L-ascorbyl phosphate.

6. The polynucleotide sodium hyaluronate gel according to claim 1, characterized in that: The stabilizer comprises a mixture of carbomer and vitamin E.

7. A method for preparing a polynucleotide sodium hyaluronate gel according to any one of claims 1 to 6, characterized in that: The following steps are involved: First, add mannitol to the buffer solution, stir until completely dissolved, filter using an aqueous filter membrane, and sterilize the filtrate at 121°C for 30 minutes to obtain a mixed solution; Add polynucleotide, sodium hyaluronate powder, moisturizer, whitening agent, stabilizer to the mixed solution, shake it slowly, centrifuge it, and finally sterilize it with wet heat to obtain a gel containing polynucleotide sodium hyaluronate.

8. The method for preparing a polynucleotide sodium hyaluronate gel according to claim 7, characterized in that: The buffer solution is a mixed solution consisting of disodium hydrogen phosphate dodecahydrate, sodium dihydrogen phosphate dihydrate, sodium chloride and water for injection.

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