Filling equipment for efficient amplification kit

Abstract

The invention relates to the technical field of biological experiment equipment, and provides efficient amplification kit filling equipment which comprises a base. The positioning frame is located above the base, the positioning frame is arranged in a lifting movement mode relative to the base, the positioning frame is provided with a containing groove, the containing groove is used for containing a kit, and the bottom end of the kit abuts against the base; the liquid storage part is located above the positioning frame, the liquid storage part is arranged in a lifting moving mode relative to the positioning frame, the liquid storage part is provided with a branch liquid storage cavity, the bottom of the branch liquid storage cavity is provided with a vertical sliding way, and the vertical sliding way communicates with the branch liquid storage cavity; the liquid outlet head is arranged in the vertical sliding way in a sliding mode, the liquid outlet head is provided with a liquid outlet flow channel, the liquid outlet flow channel is provided with a liquid inlet and a liquid outlet, and after the liquid outlet head is configured to slide in a lifting mode, the liquid outlet flow channel is communicated with the branch liquid storage cavity through the liquid inlet or is not communicated with the branch liquid storage cavity through the liquid outlet. By means of the technical scheme, the problem that in the prior art, when the standby amount of a kit is not enough in the actual experiment operation process, the instant experiment requirement cannot be met is solved.

Description

Technical Field

[0001] The invention relates to the technical field of biological experiment equipment, and in particular to a filling device for a high-efficiency amplification reagent kit. Background Art

[0002] The high-efficiency expansion kit is an important tool that plays a key role in the biomedical field, especially in immune cell therapy research and clinical applications. The kit is mainly used to promote and enhance the expansion of natural killer cells, thereby providing strong support for related disease treatment and immune research. In actual use, the kit needs to be accurately perfused with specific cytokine base fluid and basal culture medium.

[0003] Typically, laboratories and medical institutions purchase batches of filled test kits for use. However, in the actual experimental operation process, some problems are often encountered. Due to the complexity and uncertainty of the experiment, there may be insufficient spare quantity to meet the immediate experimental needs. In addition, different experiments may have special requirements for the material composition or content of the solution inside the test kit, and batch-produced filled test kits are difficult to flexibly respond to these changes. When the substance or content of the internal solution needs to change, it is difficult to quickly obtain batches of test kits that meet specific requirements, which to a certain extent affects the progress and effect of the experiment. In view of the above situation, there is an urgent need for a filling device that can stably, cleanly and quantitatively perform temporary filling of high-efficiency amplification test kits when needed, so as to meet the diversity and immediacy of the experiment and improve the efficiency and flexibility of the experiment. Summary of the invention

[0004] The present invention provides a filling device for an efficient amplification reagent kit, which solves the problem in the related art that when the spare amount of the reagent kit is insufficient during the actual experimental operation, the immediate experimental demand cannot be met.

[0005] The technical solution of the present invention is as follows: A filling device for a high-efficiency amplification kit, used for filling the inside of the kit, comprising: Pedestal; A positioning frame, the positioning frame is located above the base, the positioning frame is arranged to move relative to the base, the positioning frame has a placement groove, the placement groove is used to accommodate the reagent kit, and the bottom end of the reagent kit abuts against the base; A liquid storage member, the liquid storage member is located above the positioning frame, the liquid storage member is arranged to move relative to the positioning frame, the liquid storage member has a sub-liquid storage cavity, the bottom of the sub-liquid storage cavity has a vertical slide, and the vertical slide is connected to the sub-liquid storage cavity; A liquid outlet head is slidably arranged in a vertical slideway, and the liquid outlet head has a liquid outlet channel, and the liquid outlet channel has a liquid flow inlet and a liquid flow outlet. After the liquid outlet head is configured to rise and fall and slide, the liquid outlet channel is connected to or disconnected from the sub-liquid storage cavity through the liquid flow inlet.

[0006] As a further technical solution, the top of the liquid outlet head is provided with a blocking portion, and the liquid flow inlet is located on one side of the blocking portion. After the liquid outlet head slides down, the blocking portion blocks the vertical slide. The liquid outlet head also has a pushed portion. After the liquid storage component drives the liquid outlet head to slide down, the liquid flow inlet is used to extend into the interior of the reagent box, and the pushed portion abuts against the upper end of the reagent box, driving the liquid outlet head to slide up, thereby allowing the blocking portion to stop blocking the vertical slide, and the liquid outlet channel is connected with the sub-liquid storage cavity through the liquid flow inlet.

[0007] As a further technical solution, the sub-liquid storage chamber has a sub-liquid inlet, and further comprises: A liquid separation plate, the liquid separation plate is arranged in the liquid storage member, the liquid separation plate has a main liquid storage cavity, and the main liquid storage cavity has a main liquid inlet and a liquid distribution port; A quantitative disk is rotatably arranged in the sub-liquid storage cavity, the quantitative disk has a liquid transfer groove, the liquid distribution port and the sub-liquid storage cavity are staggered in the circumferential direction, and the quantitative disk is configured so that after rotation, the liquid transfer groove is connected with the liquid distribution port and the sub-liquid storage cavity in sequence.

[0008] As a further technical solution, the side wall of the placement groove is provided with a horizontal slide groove, and the horizontal slide groove is symmetrically arranged on both sides of the placement groove, and further includes: The first clamping members, which are two in number, are respectively slidably disposed in the horizontal slide groove, and the first clamping members are configured to move closer to or farther from each other after sliding. After moving closer to each other, the two first clamping members respectively abut against the side walls on both sides of the reagent box.

[0009] As a further technical solution, it also includes an anti-deflection bracket, the anti-deflection bracket has a vertical placement space, the placement space is used to place the reagent kit, the anti-deflection bracket is arranged on the base, the anti-deflection bracket has a vertical guiding inclined groove, and further includes: The second clamping member is located below the first clamping member, the second clamping member has a sliding portion, the sliding portion is slidably arranged in the guide bevel, the guide bevel and the second clamping member are arranged in pairs, and the two second clamping members are configured to move away from or approach each other after lifting and sliding.

[0010] As a further technical solution, the anti-deflection bracket further has a horizontally opened strip guide groove, and further comprises: A first connecting rod, one end of which is hinged to the first clamping member, and the other end of which is hinged to the second clamping member, and the first connecting rod passes through and is slidably disposed in the strip guide groove, after the positioning frame slides down, the first connecting rod abuts against the side wall of the strip guide groove, and drives the first clamping member to swing toward the direction close to the reagent box.

[0011] As a further technical solution, it also includes: A telescopic rod, one end of which is arranged on the base, and the other end is arranged on the liquid storage member, and the telescopic rod passes through the positioning frame, the telescopic rod has a stopper, and the stopper is located between the liquid storage member and the positioning frame, and the telescopic rod is configured so that after the liquid storage member slides down, the telescopic rod is driven to retract, and the stopper is brought into contact with the positioning frame, thereby driving the positioning frame to slide down synchronously.

[0012] As a further technical solution, it also includes: A first elastic member, wherein one end of the first elastic member acts on the positioning frame, and the other end of the first elastic member acts on the liquid storage member to provide a force for the liquid storage member to move away from the positioning frame.

[0013] As a further technical solution, the anti-deflection bracket, the first clamp, the second clamp, and the first connecting rod together constitute a straightening assembly, and the straightening assembly is arranged in a circle. The placement groove, the sub-liquid storage chamber, the liquid distribution port and the liquid outlet head are all arranged in a circle, and the number of the two is the same as the straightening assembly.

[0014] As a further technical solution, the upper end of the liquid storage member has scale lines, and the number of the scale lines is the same as the number of the righting components, and further includes: A rotating transmission member is arranged on the rotating shaft of the quantitative disk. The rotating transmission member has a pointer part, and the pointer part is aligned with or cancels the alignment with the scale line after rotation.

[0015] The working principle and beneficial effects of the present invention are: In the present invention, the base is placed horizontally on the ground or a workbench, and the positioning frame is installed above the base through a guide column and a lifting drive device or manually, so as to realize lifting and moving relative to the base. The liquid storage part is installed above the positioning frame through another set of guide columns and a lifting structure. The liquid discharge head cooperates with the vertical slide and can slide up and down in the vertical slide. First, the reagent box to be filled is placed in the placement groove of the positioning frame. Then, the liquid storage part descends, driving the liquid discharge head to move together. When the liquid discharge head descends to the point where the liquid flow inlet of the liquid discharge channel is connected with the sub-liquid storage cavity, the liquid in the liquid storage cavity is poured into the reagent box through the liquid flow outlet of the liquid discharge channel. After the filling is completed, the liquid storage part rises, and the liquid discharge head rises accordingly, and the liquid flow inlet of the liquid discharge channel is disconnected from the sub-liquid storage cavity, and the filling is stopped. The filling amount is automatically controlled by the equipment, which avoids the errors that may occur in manual filling, improves the accuracy and consistency of filling, and provides convenience for subsequent experiments. The equipment has a compact structure and occupies a small space, which is convenient for use and placement in places with limited space such as laboratories. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] The preferred implementation modes will be described below in a clear and understandable manner with reference to the accompanying drawings to further illustrate the above-mentioned characteristics, technical features, advantages and implementation methods of the present invention.

[0017] Figure 1 This is a schematic diagram of the overall external structure of the present invention; Figure 2 It is another perspective structural schematic diagram of the present invention; Figure 3 for Figure 2 Schematic diagram of the internal structure; Figure 4 for Figure 3 A partial enlarged structural diagram of the middle part; Figure 5 for Figure 2 A schematic diagram of the internal structure from another perspective; Figure 6 It is a schematic diagram of the structure of the liquid outlet head in the present invention; Figure 7 It is a schematic diagram of the structure of the righting assembly in the present invention.

[0018] In the figure: base 1, positioning frame 2, placement slot 201, horizontal slide 202, liquid storage member 3, sub-liquid storage chamber 301, vertical slide 302, sub-liquid inlet 303, scale line 304, liquid outlet head 4, liquid outlet channel 401, liquid inlet 402, liquid outlet 403, blocking part 404, pushed part 405, liquid separation plate 5, main liquid storage chamber 501, main liquid inlet 502 , liquid preparation port-503, quantitative disk-6, pipetting slot-601, first clamping member-7, anti-deflection bracket-8, straightening assembly-800, placement space-801, guide inclined groove-802, strip guide groove-803, second clamping member-9, sliding part-901, first connecting rod-10, telescopic rod-11, blocking part-1101, first elastic member-12, rotating transmission member-13, pointer part-1301. DETAILED DESCRIPTION

[0019] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the specific implementation methods of the present invention will be described below with reference to the accompanying drawings. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings and other implementation methods can be obtained based on these drawings without creative work.

[0020] In order to simplify the drawings, only the parts related to the invention are schematically shown in each figure, and they do not represent the actual structure of the product. In addition, in order to simplify the drawings and facilitate understanding, in some figures, only one of the parts with the same structure or function is schematically shown, or only one of them is marked. In this article, "one" not only means "only one", but also means "more than one", and "several" includes "two" and "more than two".

[0021] In this document, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0022] In addition, in the description of the present application, the terms "first", "second", etc. are only used to distinguish the description and cannot be understood as indicating or implying relative importance.

[0023] Reference Figure 1 to Figure 7The first embodiment of the present invention provides a filling device for an efficient amplification reagent kit, which is used for filling the inside of the reagent kit, and includes a base 1; a positioning frame 2 is located above the base 1, and the positioning frame 2 is arranged to move up and down relative to the base 1, and the positioning frame 2 has a placement groove 201, and the placement groove 201 is used to accommodate the reagent kit, and the bottom end of the reagent kit abuts the base 1; a liquid storage member 3 is located above the positioning frame 2, and the liquid storage member 3 is arranged to move up and down relative to the positioning frame 2, and the liquid storage member 3 has a sub-liquid storage cavity 301, and the bottom of the sub-liquid storage cavity 301 has a vertical slide 302, and the vertical slide 302 is connected to the sub-liquid storage cavity 301; a liquid outlet head 4 is slidably arranged in the vertical slide 302, and the liquid outlet head 4 has a liquid outlet flow channel 401, and the liquid outlet flow channel 401 has a liquid flow inlet 402 and a liquid flow outlet 403. After the liquid outlet head 4 is configured to slide up and down, the liquid outlet flow channel 401 is connected or disconnected with the sub-liquid storage cavity 301 through the liquid flow inlet 402.

[0024] In this embodiment, the base 1 is placed horizontally on the ground or on a workbench, and the positioning frame 2 is installed above the base 1 through a guide column and a lifting drive device or manually, so as to realize lifting and moving relative to the base 1. The liquid storage part 3 is installed above the positioning frame 2 through another set of guide columns and a lifting structure. The liquid discharge head 4 cooperates with the vertical slide 302 and can slide up and down in the vertical slide 302. First, the reagent kit to be filled is placed in the placement groove 201 of the positioning frame 2. Then, the liquid storage part 3 descends, driving the liquid discharge head 4 to move together. When the liquid discharge head 4 descends to the liquid flow inlet 402 of the liquid discharge channel 401 and communicates with the sub-liquid storage chamber 301, the liquid in the liquid storage chamber 301 is poured into the reagent kit through the liquid flow outlet 403 of the liquid discharge channel 401. After the filling is completed, the liquid storage part 3 rises, and the liquid discharge head 4 rises accordingly, and the liquid flow inlet 402 of the liquid discharge channel 401 is disconnected from the sub-liquid storage chamber 301, and the filling is stopped. The filling volume is automatically controlled by the equipment, avoiding the possible errors caused by manual filling, improving the filling accuracy and consistency, and providing convenience for subsequent experiments. The equipment has a compact structure and occupies a small space, making it easy to use and place in laboratories and other places with limited space.

[0025] Furthermore, the top of the liquid outlet head 4 has a blocking portion 404, and the liquid flow inlet 402 is located on one side of the blocking portion 404. After the liquid outlet head 4 slides down, the blocking portion 404 blocks the vertical slide 302. The liquid outlet head 4 also has a pushed portion 405. After the liquid storage component 3 drives the liquid outlet head 4 to slide down, the liquid flow inlet 402 is used to extend into the interior of the reagent box, and the pushed portion 405 abuts against the upper end of the reagent box, driving the liquid outlet head 4 to slide up, thereby causing the blocking portion 404 to stop blocking the vertical slide 302, and the liquid outlet channel 401 is connected with the sub-liquid storage chamber 301 through the liquid flow inlet 402.

[0026] In this embodiment, the liquid storage part 3 drives the liquid outlet head 4 to slide downward, and the liquid flow inlet 402 of the liquid outlet head 4 extends into the interior of the reagent box. At this time, the pushed part 405 abuts against the upper end of the reagent box. Due to the obstruction of the reagent box, the liquid outlet head 4 is driven to slide relatively upward, so that the blocking part 404 no longer blocks the vertical slide 302, and the liquid outlet channel 401 is connected with the sub-liquid storage chamber 301 through the liquid flow inlet 402, and filling begins. The design of the blocking part 404 can effectively prevent liquid leakage when not filling, ensure the sealing and safety of the equipment in a non-working state, and bring convenience to operation. The interaction between the pushed part 405 and the reagent box realizes the automatic connection control of the liquid outlet head 4, without the need for additional complex operations, and improves the convenience and automation of filling. This automatic connection mechanism can accurately start filling when the reagent box is in place, reducing the time and error of manual intervention and improving the filling efficiency. The trigger filling method, when the reagent kit placed on the equipment is not full, only the workstation where the reagent kit is placed is filled, saving liquid medicine and avoiding unnecessary waste. At the same time, it also meets the needs of immediate use without large-scale filling, increasing the flexibility and practicality of filling.

[0027] Furthermore, the sub-liquid storage chamber 301 has a sub-liquid inlet 303, and also includes a liquid separation plate 5, which is arranged in the liquid storage component 3, and the liquid separation plate 5 has a main liquid storage chamber 501, and the main liquid storage chamber 501 has a main liquid inlet 502 and a liquid distribution port 503; the quantitative plate 6 is rotatably arranged in the sub-liquid storage chamber 301, and the quantitative plate 6 has a liquid transfer groove 601, and the liquid distribution port 503 and the sub-liquid storage chamber 301 are staggered in the circumferential direction. The quantitative plate 6 is configured so that after rotation, the liquid transfer groove 601 is connected with the liquid distribution port 503 and the sub-liquid storage chamber 301 in sequence.

[0028] In this embodiment, liquid enters the main liquid storage chamber 501 from the main liquid inlet 502, and then flows out from the liquid dispensing port 503. The quantitative disk 6 rotates, and when the liquid transfer slot 601 rotates to align with the liquid dispensing port 503, the liquid enters the liquid transfer slot 601. As the quantitative disk 6 continues to rotate, the liquid transfer slot 601 is connected with the sub-liquid storage chamber 301, and the quantitative liquid is sent into the sub-liquid storage chamber 301. The capacity in the liquid transfer slot is fixed here, such as 2ml. The amount of solution transferred in each transfer process is fixed. Multiple quantitative injections can be achieved by the number of rotations of the quantitative disk. The cooperation of the liquid transfer disk 5 and the quantitative disk 6 greatly improves the quantitative accuracy of the filling, which is conducive to the standardization and repeatability of the experiment. The filling amount is controlled by the number of rotations of the quantitative disk 6. The operation is simple and intuitive, and it is easy to realize automatic control, which improves work efficiency. This fixed capacity and repeatable quantitative method effectively avoids the error of the filling amount, ensures the consistency of the amount of liquid medicine in each test kit, and provides a strong guarantee for the reliability of the experimental results. Due to the precise control of the filling volume, the waste of liquid medicine is reduced, the experimental cost is reduced, and it is more environmentally friendly. For different filling volume requirements, only the number of rotations of the quantitative disk 6 needs to be calculated and controlled, which increases the flexibility and adaptability of the equipment and can meet the filling requirements under various experimental conditions.

[0029] Furthermore, the side wall of the placement groove 201 is provided with a horizontal slide groove 202, which is symmetrically arranged on both sides of the placement groove 201, and also includes a first clamping member 7. There are two first clamping members 7, which are respectively slidably arranged in the horizontal slide groove 202, and the first clamping members 7 are configured to move closer to or away from each other after sliding. After moving closer to each other, the two first clamping members 7 respectively abut against the side walls on both sides of the test kit.

[0030] In this embodiment, the side walls of the placement groove 201 are symmetrically provided with horizontal slide grooves 202, and the two first clamps 7 are respectively installed in the horizontal slide grooves 202 on both sides and can slide therein. The first clamp 7 is a claw-shaped structure with certain elasticity and friction. After the reagent box is placed in the placement groove 201, the two first clamps 7 are pushed to slide in the horizontal slide groove 202, so that they are close to each other until they are closely abutted against the side walls on both sides of the reagent box, thereby fixing the reagent box in the center position of the placement groove 201. It is ensured that the reagent box will not be displaced in the horizontal direction during the filling process, the accuracy of the filling position is guaranteed, and the filling quality is improved. The operation is simple and convenient, and the reagent box can be fixed by manual pushing without complicated operation steps. The close abutment of the first clamp 7 with the side wall of the reagent box increases the friction force and further improves the stability of the reagent box. The symmetrically arranged structure makes the reagent box evenly stressed when fixed, avoiding tilting or damage caused by uneven stress. The reagent box can be quickly fixed, and the efficiency of filling is improved, which is particularly suitable for the scene of batch filling.

[0031] Furthermore, it also includes an anti-deflection bracket 8, which has a vertical placement space 801, and the placement space 801 is used to place the test kit. The anti-deflection bracket 8 is arranged on the base 1, and the anti-deflection bracket 8 has a vertical guiding bevel 802. It also includes a second clamping member 9, and the second clamping member 9 is located below the first clamping member 7. The second clamping member 9 has a sliding portion 901, and the sliding portion 901 is slidably arranged in the guiding bevel 802. The guiding bevel 802 and the second clamping member 9 are arranged in pairs, and the two second clamping members 9 are configured to move away from or approach each other after lifting and sliding.

[0032] In this embodiment, after the reagent box is placed in the placement space 801, as the positioning frame 2 descends, the first clamp 7 drives the second clamp 9 to descend together. Due to the effect of the guide chute 802, the sliding parts 901 of the two second clamps 9 slide in the guide chute 802, so that the two second clamps 9 are close to each other, thereby further fixing the reagent box from the bottom to prevent it from deflecting during the filling process. The cooperation of the anti-deflection bracket 8 and the second clamp 9 provides double fixing and anti-deflection measures up and down, greatly improving the stability and accuracy of the reagent box during the filling process. The setting of the guide chute 802 converts the vertical movement into the horizontal approaching movement, realizes the automatic clamping of the bottom of the reagent box, does not require an additional driving device, and simplifies the structure and operation. It can adapt to the size and shape errors of the reagent box, and through the adaptive movement of the second clamp 9, it is ensured that effective anti-deflection fixation can be provided for reagent boxes of various specifications. It effectively avoids the position deviation of the reagent box caused by vibration or other factors during the filling process, and ensures the accuracy and quality of the filling.

[0033] Furthermore, the anti-bias bracket 8 also has a horizontally opened strip guide groove 803, and also includes a first connecting rod 10, one end of the first connecting rod 10 is hinged to the first clamp 7, and the other end is hinged to the second clamp 9, and the first connecting rod 10 passes through and is slidably set in the strip guide groove 803. After the positioning frame 2 slides down, the first connecting rod 10 abuts against the side wall of the strip guide groove 803, and drives the first clamp 7 to swing toward the direction close to the test kit.

[0034] In this embodiment, when the positioning frame 2 slides downward, the first clamp 7 and the second clamp 9 are driven downward. Since the first connecting rod 10 passes through the strip guide groove 803, when it is lowered to a certain extent, the first connecting rod 10 abuts against the side wall of the strip guide groove 803, thereby driving the first clamp 7 to swing toward the direction close to the reagent box, further strengthening the fixation of the reagent box. Through the cooperation of the first connecting rod 10 and the strip guide groove 803, the first clamp 7 is automatically driven to swing and clamp the reagent box when the positioning frame 2 is lowered, thereby improving the degree of automation and efficiency of fixation. The reagent box can be fixed more tightly, especially the fixing effect is gradually enhanced during the lowering process of the positioning frame 2, and the stability of the reagent box during the filling process is improved. The structure is simple and reliable, which reduces the possibility of failure and reduces the maintenance cost of the equipment.

[0035] Furthermore, it also includes a telescopic rod 11, one end of the telescopic rod 11 is arranged on the base 1, and the other end is arranged on the liquid storage member 3, and the telescopic rod 11 passes through the positioning frame 2, the telescopic rod 11 has a stopper 1101, and the stopper 1101 is located between the liquid storage member 3 and the positioning frame 2. The telescopic rod 11 is configured so that after the liquid storage member 3 slides down, the telescopic rod 11 is driven to retract, and the stopper 1101 is brought into contact with the positioning frame 2, thereby driving the positioning frame 2 to slide down synchronously.

[0036] In this embodiment, when the liquid storage part 3 slides down, the telescopic rod 11 is driven to retract. When it retracts to a certain extent, the stopper 1101 abuts against the positioning frame 2, thereby driving the positioning frame 2 to slide down synchronously. The setting of the telescopic rod 11 realizes the linkage of the downward movement of the liquid storage part 3 and the positioning frame 2, simplifies the control process of the equipment, and improves the convenience of operation. The synchronization of the downward movement of the liquid storage part 3 and the positioning frame 2 is ensured, making the filling process more coordinated and stable, and improving the accuracy and quality of filling. The telescopic rod 11 is set to hover at any position. The design principle of this hovering is based on the damping and locking mechanism inside the telescopic rod 11. Through the internal damping structure, the telescopic speed of the telescopic rod 11 can be controlled so that it can perform telescopic movements smoothly. The locking mechanism can provide sufficient resistance and support force when the telescopic rod 11 reaches any position, so that it remains in the current position and achieves hovering.

[0037] Furthermore, a first elastic member 12 is included. One end of the first elastic member 12 acts on the positioning frame 2 , and the other end of the first elastic member 12 acts on the liquid storage member 3 to provide a force to move the liquid storage member 3 away from the positioning frame 2 .

[0038] In this embodiment, when the liquid storage member 3 is lowered for filling, the first elastic member 12 is compressed to store elastic potential energy. After the filling is completed, the first elastic member 12 releases the elastic potential energy, providing a force to move the liquid storage member 3 away from the positioning frame 2, so that the liquid storage member 3 rises and resets. The setting of the first elastic member 12 provides automatic power for the liquid storage member 3 to rise and reset, reduces additional driving devices, and simplifies the equipment structure and control process.

[0039] Furthermore, the anti-deviation bracket 8, the first clamp 7, the second clamp 9, and the first connecting rod 10 together constitute the straightening assembly 800, and the straightening assembly 800 is arranged in a circle. There are several placement grooves 201, sub-liquid storage chambers 301, liquid distribution ports 503 and liquid outlet heads 4 arranged in a circle, and the number of them is the same as the straightening assembly 800.

[0040] In this embodiment, during the filling process, multiple straightening assemblies 800 straighten and fix multiple test kits at the same time to ensure that the position of each test kit is accurate and stable. The various components work together to achieve the filling operation of multiple test kits at the same time. Despite the small-batch production, the provision of multiple circularly arranged straightening assemblies 800 can still significantly improve the filling efficiency, saving operation time compared to a single filling station. The circular arrangement design can still make the equipment relatively uniformly stressed during operation, reduce the deviation caused by local uneven stress, and ensure the accuracy and quality of small-batch filling. Processing multiple test kits at a time can better utilize the limited space and resources of the equipment under small-batch requirements and improve the comprehensive utilization efficiency of the equipment. The number and arrangement of each component are matched to ensure the relative synchronization and consistency of small-batch filling operations, so that the product quality is relatively uniform. It is convenient to carry out local modular maintenance. When individual straightening assemblies or filling stations fail, they can be repaired or replaced in a targeted manner without affecting the progress of the overall small-batch production.

[0041] Furthermore, the upper end of the liquid storage part 3 has scale lines 304, and the number of scale lines 304 is also the same as the number of the straightening components 800. It also includes a rotating transmission part 13, which is arranged on the rotating axis of the quantitative disk 6. The rotating transmission part 13 has a pointer part 1301, and the pointer part 1301 is aligned with the scale lines 304 or cancels the alignment after rotation.

[0042] In the present embodiment, when it is necessary to adjust the rotation angle of the liquid separation disk 5 to control the liquid separation amount, the rotating transmission member 13 can be driven manually or electrically to rotate. The rotating transmission member 13 drives the quantitative disk 6 to rotate so that the pointer portion 1301 is aligned with the corresponding scale line 304, thereby accurately setting the liquid separation amount. The cooperation of the scale line 304 and the pointer portion 1301 provides an intuitive reference for the adjustment of the liquid separation amount, which is convenient for the operator to quickly and accurately set the liquid separation amount according to the experimental requirements. The manual or electrically controlled driving mode increases the flexibility of the equipment and can adapt to the needs of different experimental scenes and operating habits. In small batch experiments, this precise liquid separation amount control helps to improve the repeatability and accuracy of the experiment and ensure the reliability of the experimental results.

[0043] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the claims of the present invention.

Claims

1. A filling device for a high-efficiency amplification kit, used for filling the inside of the kit, characterized in that: include: Base (1); a positioning frame (2), the positioning frame (2) being located above the base (1), the positioning frame (2) being arranged to be lifted and moved relative to the base (1), the positioning frame (2) having a placement groove (201), the placement groove (201) being used to accommodate the reagent kit, and the bottom end of the reagent kit abuts against the base (1); A liquid storage member (3), the liquid storage member (3) being located above the positioning frame (2), the liquid storage member (3) being arranged to be lifted and moved relative to the positioning frame (2), the liquid storage member (3) having a sub-liquid storage cavity (301), the bottom of the sub-liquid storage cavity (301) having a vertical slideway (302), the vertical slideway (302) being in communication with the sub-liquid storage cavity (301); A liquid outlet head (4), the liquid outlet head (4) being slidably disposed in a vertical slideway (302), the liquid outlet head (4) having a liquid outlet channel (401), the liquid outlet channel (401) having a liquid flow inlet (402) and a liquid flow outlet (403), the liquid outlet head (4) being configured such that after the liquid outlet head (4) is lifted and slid, the liquid outlet channel (401) is connected to or disconnected from the sub-liquid storage chamber (301) through the liquid flow inlet (402).

2. The filling device of the high-efficiency amplification kit according to claim 1, characterized in that: The top of the liquid outlet head (4) has a blocking portion (404), and the liquid flow inlet (402) is located on one side of the blocking portion (404). After the liquid outlet head (4) slides down, the blocking portion (404) blocks the vertical slideway (302). The liquid outlet head (4) also has a pushed portion (405). After the liquid storage member (3) drives the liquid outlet head (4) to slide downward, the liquid flow inlet (402) is used to extend into the interior of the reagent box, and the pushed portion (405) abuts against the upper end of the reagent box, driving the liquid outlet head (4) to slide upward, thereby causing the blocking portion (404) to stop blocking the vertical slideway (302), and the liquid outlet flow channel (401) is connected to the sub-liquid storage chamber (301) through the liquid flow inlet (402).

3. The filling device of the high-efficiency amplification kit according to claim 1, characterized in that: The sub-liquid storage chamber (301) has a sub-liquid inlet (303) and further comprises: A liquid separation plate (5), the liquid separation plate (5) being arranged in the liquid storage element (3), the liquid separation plate (5) having a main liquid storage cavity (501), the main liquid storage cavity (501) having a main liquid inlet (502) and a liquid distribution port (503); A quantitative disk (6), the quantitative disk (6) being rotatably disposed in the sub-liquid storage chamber (301), the quantitative disk (6) having a liquid transfer groove (601), the liquid dispensing port (503) and the sub-liquid storage chamber (301) being staggered in the circumferential direction, and the quantitative disk (6) being configured such that after rotation, the liquid transfer groove (601) is in sequential communication with the liquid dispensing port (503) and the sub-liquid storage chamber (301).

4. The filling device of the high-efficiency amplification kit according to claim 3, characterized in that: The side wall of the placement groove (201) is provided with a horizontal slide groove (202), and the horizontal slide groove (202) is symmetrically arranged on both sides of the placement groove (201), and further comprises: The first clamping members (7) are two in number and are respectively slidably disposed in the horizontal slide groove (202). The first clamping members (7) are configured to move closer to or farther from each other after sliding. After moving closer to each other, the two first clamping members (7) respectively abut against the side walls on both sides of the reagent box.

5. The filling device of the high-efficiency amplification kit according to claim 4, characterized in that: It also includes an anti-deflection bracket (8), the anti-deflection bracket (8) having a vertical placement space (801), the placement space (801) being used to place the reagent kit, the anti-deflection bracket (8) being arranged on the base (1), the anti-deflection bracket (8) having a vertical guiding inclined groove (802), and further includes: A second clamping member (9), the second clamping member (9) is located below the first clamping member (7), the second clamping member (9) has a sliding portion (901), the sliding portion (901) is slidably arranged in the guide inclined groove (802), the guide inclined groove (802) and the second clamping member (9) are arranged in pairs, and the two second clamping members (9) are configured to move away from or approach each other after lifting and sliding.

6. The filling equipment of the high-efficiency amplification kit according to claim 5, characterized in that: The anti-deflection bracket (8) further comprises a horizontally arranged strip-shaped guide groove (803), and further comprises: A first connecting rod (10), one end of the first connecting rod (10) is hinged to the first clamping member (7), and the other end is hinged to the second clamping member (9), and the first connecting rod (10) passes through and is slidably arranged in the strip guide groove (803), after the positioning frame (2) slides downward, the first connecting rod (10) abuts against the side wall of the strip guide groove (803), and drives the first clamping member (7) to swing in a direction close to the reagent box.

7. The filling equipment of the high-efficiency amplification kit according to claim 1, characterized in that: Also includes: A telescopic rod (11), one end of the telescopic rod (11) being arranged on the base (1), and the other end of the telescopic rod (11) being arranged on the liquid storage member (3), and the telescopic rod (11) passing through the positioning frame (2), the telescopic rod (11) having a stopper (1101), the stopper (1101) being located between the liquid storage member (3) and the positioning frame (2), the telescopic rod (11) being configured such that, after the liquid storage member (3) slides down, the telescopic rod (11) is driven to retract, and the stopper (1101) is brought into contact with the positioning frame (2), thereby driving the positioning frame (2) to slide down synchronously.

8. The filling equipment of the high-efficiency amplification kit according to claim 1, characterized in that: Also includes: A first elastic member (12), wherein one end of the first elastic member (12) acts on the positioning frame (2) and the other end of the first elastic member (12) acts on the liquid storage member (3) to provide a force for moving the liquid storage member (3) away from the positioning frame (2).

9. The filling device of the high-efficiency amplification kit according to claim 6, characterized in that: The anti-deflection bracket (8), the first clamping member (7), the second clamping member (9), and the first connecting rod (10) together constitute a straightening assembly (800), and the straightening assembly (800) is arranged in a plurality of circles. The placement groove (201), the sub-liquid storage chamber (301), the liquid distribution port (503), and the liquid outlet head (4) are all arranged in a plurality of circles, and the number of the plurality of the straightening assemblies (800) is the same as that of the straightening assemblies (800).

10. The filling device of the high-efficiency amplification kit according to claim 9, characterized in that: The upper end of the liquid storage member (3) has scale lines (304), the number of the scale lines (304) is the same as the number of the righting assemblies (800), and further comprises: A rotating transmission member (13), the rotating transmission member (13) being arranged on a rotating shaft of the quantitative disk (6), the rotating transmission member (13) having a pointer portion (1301), the pointer portion (1301) being aligned with or de-aligned with the scale line (304) after rotation.

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