Kit for rapidly detecting HPV (human papillomavirus) through isothermal amplification

Abstract

The invention relates to the field of HPV (human papillomavirus) kits, in particular to an isothermal amplification rapid HPV detection kit which comprises a kit body, a kit cover, a refrigerator, a cooling cylinder and the like. A battery is arranged in the box body; the box body is detachably connected with a box cover; a plurality of refrigerators are fixedly connected in the box body; the box body is connected with cooling cylinders matched with the refrigerators in number. Through cooperation of a refrigerator, a cooling cylinder, a plug and a pushing system, only the refrigerator corresponding to the cooling cylinder filled with samples works, so that the problem that a large amount of energy is wasted due to the fact that an existing kit can only cool the whole interior and cannot independently cool test tubes filled with samples is solved, and meanwhile, the energy consumption is reduced. According to the kit, the prompt plate is matched with the pushing system, so that only the prompt plate corresponding to the cooling cylinder filled with the sample is exposed outside the kit body, and the problem that when an existing kit is used, a medical worker easily takes a test tube filled with the sample, namely the medical worker easily takes the test tube repeatedly is solved.

Description

Technical Field

[0001] The present invention relates to the field of HPV test kits, and in particular to a kit for rapid detection of HPV by isothermal amplification. Background Art

[0002] The existing isothermal amplification rapid detection of HPV (human papillomavirus) is an efficient and simple technology that can complete the amplification and detection of HPV DNA in a relatively short time. However, the HPV virus test samples extracted are generally placed in a test tube inside the test kit. When loading the sample, the medical staff first opens the test kit, then removes the test tube, and places the sample into the test tube, and finally puts the test tube containing the sample back into the test kit. However, since the test kit contains multiple test tubes, and each test tube has the same shape, when taking the reagent, medical staff are likely to take the test tube that has already been filled with the sample, which easily leads to medical staff repeatedly taking the test tube; In addition, existing test kits are usually equipped with refrigeration equipment to ensure the stability of samples and the accuracy of test results by lowering the temperature of test tubes. However, since the existing refrigeration equipment can only cool the entire interior of the test kit, that is, the test tubes containing samples and the test tubes without samples will be cooled together, it is impossible to cool the test tubes containing samples separately, which will result in a large amount of energy waste. Summary of the Invention

[0003] In order to overcome the shortcomings of existing test kits that medical personnel can easily pick up test tubes containing samples when using them, which easily leads to medical personnel repeatedly taking test tubes, and at the same time, the existing test kits can only cool the entire interior and cannot cool the test tubes containing samples separately, resulting in a large amount of energy waste, the present invention provides a constant temperature amplification rapid detection kit for HPV.

[0004] The technical solution is: a constant temperature amplification rapid detection HPV kit, including a box body and a box cover; a battery is provided in the box body; the box body is detachably connected to the box cover; it also includes a refrigerator, a cooling cylinder, a plug, a prompt plate and a pushing system; a plurality of refrigerators are fixedly connected to the box body; the box body is connected to a cooling cylinder matching the number of refrigerators, and an insulation layer is provided on the outside of the cooling cylinder; the box body is connected to a pushing system; the pushing system is connected to a plug matching the number of refrigerators, and all plugs are connected to the battery in the box body through cables; the pushing system is connected to a prompt plate matching the number of refrigerators, and the prompt plate is slidably connected to the box body; the pushing system is used to push the plug into the refrigerator and push the prompt plate to move and expose it to the outside of the box body.

[0005] As a further preferred solution, the pushing system includes a sliding plate 1, a spring member 1, a wedge block 1, a sliding plate 2, a spring member 2, a wedge block 2, a sliding block, a trapezoidal block, a flexible plate, a winding assembly and a pulling assembly; the box body is slidably connected to a sliding plate 1 that matches the number of plugs, and each sliding plate 1 is fixedly connected to the corresponding plug; each sliding plate 1 is fixedly connected to at least one spring member 1, and all spring members 1 are fixedly connected to the box body; each sliding plate 1 is fixedly connected to a wedge block 1; the box body is slidably connected to a sliding plate 2 that matches the number of prompt plates, and each The sliding plates 2 are all fixedly connected to the corresponding prompt plates; each sliding plate 2 is fixedly connected to at least one spring component 2, and all spring components 2 are fixedly connected to the box body; each sliding plate 2 is fixedly connected to a wedge block 2; the box body is slidably connected to the sliding block; the sliding block is fixedly connected to a trapezoidal block matching the wedge block 1 and the wedge block 2; the box body is connected to a winding assembly; the winding assembly is connected to a flexible plate, and the flexible plate is fixedly connected to the sliding block; the winding assembly is used to wind up the flexible plate; the box body is connected to a pulling assembly for pulling the sliding block and the parts connected thereto to move, and the pulling assembly is connected to the sliding block.

[0006] As a further preferred solution, the winding assembly includes a mounting circular plate, a winding disk and a clockwork spring; the box body is fixedly connected to the mounting circular plate; the mounting circular plate is rotatably connected to the winding disk, and the flexible plate is wound on the winding disk; the mounting circular plate is fixedly connected to the clockwork spring, and the clockwork spring is fixedly connected to the winding disk.

[0007] As a further preferred solution, the pulling assembly includes a rotating rod, a second winding reel, a rope and a limiting unit; the box body is rotatably connected to the rotating rod; the rotating rod is slidably connected to the second winding reel through a spline structure, and the second winding reel is rotatably connected to the box body; the second winding reel is fixedly connected to the rope, and the rope is fixedly connected to the sliding block; the box body is connected to a limiting unit for limiting the rotation direction of the rotating rod, and the limiting unit is connected to the rotating rod.

[0008] As a further preferred scheme, the limiting unit includes a round block, a rotating ring, a spring component three, a support plate, a sliding rod, a spring component four, a mounting ring and a hemispherical block; the rotating rod is fixed with the round block; the round block is rotatably connected to the rotating ring; the rotating ring is fixed with the spring component three, and the spring component three is fixed to the box body; the box body is fixed with the support plate; the support plate passes through and is slidably connected to multiple sliding rods; each sliding rod is fixed with a spring component four, and all spring components four are fixed to the support plate; all sliding rods are commonly fixed with the mounting ring; the mounting ring is fixed with multiple hemispherical blocks; the round block is provided with hemispherical grooves matching the number of hemispherical blocks, and the hemispherical grooves match the shape of the hemispherical blocks.

[0009] As a further preferred solution, it further includes a limiting plate; the box body is fixedly connected to the limiting plate, and the limiting plate is slidably connected to the flexible plate.

[0010] As a further preferred embodiment, a flexible cylinder is also included; each cooling cylinder is provided with a flexible cylinder for protecting the test tube, and the open end of each flexible cylinder is wound around to the outside of the corresponding cooling cylinder and fixedly connected to the box body.

[0011] As a further preferred embodiment, it also includes a fixed plate, a pull rod, a spring telescopic rod and a ball; the box body is slidably connected to a fixed plate matching the number of cooling cylinders, and each fixed plate is fixedly connected to the corresponding cooling cylinder, and the box body and the cooling cylinder are connected together through the fixed plate; the box body is slidably connected to a pull rod matching the number of fixed plates, and each pull rod is fixedly connected to the corresponding fixed plate; the box body is fixedly connected to a spring telescopic rod matching the number of pull rods; the telescopic part of each spring telescopic rod is fixedly connected to a ball, and each ball is in contact with the corresponding pull rod, and each pull rod is provided with a spherical groove one, and each spherical groove one is aligned with the corresponding ball.

[0012] As a further preferred solution, each cooling cylinder is provided with an arc portion.

[0013] As a further preferred solution, each pull rod is provided with a second spherical groove, each second spherical groove is aligned with a corresponding sphere, and the distance between the second spherical groove and the sphere is set to be equal to the height of the flexible tube.

[0014] The technical solution provided by the present invention is that: by cooperating with the refrigerator, the cooling cylinder, the plug and the pushing system, only the refrigerator corresponding to the cooling cylinder containing the sample is in operation, thereby avoiding the problem that the existing reagent kit can only cool the entire interior and cannot cool the test tube containing the sample separately, resulting in a large amount of energy waste. At the same time, by cooperating with the prompt plate and the pushing system, only the prompt plate corresponding to the cooling cylinder containing the sample is exposed to the outside of the box body, thereby preventing the problem that medical personnel can easily pick up the test tube containing the sample when the existing reagent kit is in use, that is, the medical personnel can easily pick up the test tube repeatedly. By arranging a flexible cylinder inside the cooling cylinder, the flexible cylinder cushions the test tube containing the sample, thereby preventing the test tube containing the sample from being easily broken when it falls into the cooling cylinder, causing the sample in the test tube to overflow; The flexible tube pushes the test tube upward by cooperating with the cooling tube, the fixing plate, the pull rod, the spring telescopic rod and the ball, so that the upper end of the test tube is exposed on the upper side of the flexible tube, thereby making it easier for medical personnel to take the test tube out of the flexible tube. This prevents the problem that the medical personnel are restricted by the flexible tube due to the small diameter of the flexible tube and have difficulty in quickly removing the test tube from the flexible tube. By setting the distance between the second spherical groove and the ball to be equal to the height of the flexible tube, the cooling tube, fixed plate, pull rod, spring telescopic rod and ball cooperate with each other, and the cooling tube can push the inner side of the flexible tube to flip to the outside, so that medical staff can better disinfect the inner side of the flexible tube to prevent the problem that the diameter of the flexible tube is small and the height is large, which makes it difficult for medical staff to quickly disinfect the flexible tube. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 This is a schematic diagram of the three-dimensional structure of the isothermal amplification rapid detection kit for HPV of the present invention; Figure 2 This is a schematic diagram of the internal structure of the cartridge disclosed in the isothermal amplification rapid detection kit for HPV of the present invention; Figure 3 This is a schematic diagram of the structure of the box body, refrigerator, cooling cylinder, prompt plate, sliding plate 1, sliding plate 2, flexible plate, mounting circular plate, winding reel 1 and rope disclosed in the constant temperature amplification rapid detection kit of the present invention; Figure 4 This is a schematic diagram of the structure of the box body, refrigerator, plug, prompt plate, sliding plate 1, spring member 1, wedge block 1, sliding plate 2, spring member 2, wedge block 2, sliding block, trapezoidal block, flexible plate, mounting circular plate, winding reel 1, spring spring, and rope disclosed in the constant temperature amplification rapid detection HPV kit of the present invention; Figure 5 This is a schematic structural diagram of the box body, refrigerator, cooling cylinder, flexible cylinder, fixing plate, pull rod, spring telescopic rod and ball disclosed in the isothermal amplification rapid detection kit of HPV of the present invention; Figure 6 This is a schematic structural diagram of the box body, cooling cylinder and flexible cylinder disclosed in the isothermal amplification rapid detection kit for HPV of the present invention; Figure 7 This is a schematic structural diagram of the pulling assembly and the limiting unit disclosed in the isothermal amplification rapid detection kit for HPV of the present invention; Figure 8 This is a schematic diagram of the structure of the round block and hemispherical block disclosed in the isothermal amplification rapid detection kit for HPV of the present invention; Figure 9 This is a diagram of the state in which the upper end of the test tube disclosed in the isothermal amplification rapid detection kit for HPV of the present invention is exposed to the upper side of the flexible tube; Figure 10 This is a state diagram of the flexible tube disclosed in the isothermal amplification rapid detection kit for HPV of the present invention when the inner side is turned to the outer side.

[0016] Among them: 1-box body, 2-box cover, 3-refrigerator, 4-cooling cylinder, 5-plug, 6-prompt plate, 111-sliding plate 1, 112-spring member 1, 113-wedge block 1, 114-sliding plate 2, 115-spring member 2, 116-wedge block 2, 117-sliding block, 118-trapezoidal block, 119-flexible plate, 121-mounting circular plate, 122-winding disk 1, 123-spring spring, 131-rotating rod, 132-winding disk 2, 1 33-rope, 141-round block, 142-rotating ring, 143-spring component three, 144-support plate, 145-sliding rod, 146-spring component four, 147-mounting ring, 148-hemispherical block, 151-limiting plate, 211-flexible cylinder, 221-fixing plate, 222-pull rod, 223-spring telescopic rod, 224-ball, 401-arc part, 14101-hemispherical groove, 22201-spherical groove one, 22202-spherical groove two. DETAILED DESCRIPTION

[0017] First of all, it should be noted that in the various embodiments described, identical components are provided with identical reference numerals or identical component names, wherein the disclosure contained throughout the entire description can be transferred to the same components having the same reference numerals or identical component names. Positional designations selected in the description, such as top, bottom, lateral, etc., also refer to the directly described and illustrated figures and are transferred to the new position in the event of a change in position.

[0018] Example 1 A constant temperature amplification rapid detection kit for HPV, such as Figures 1-8 As shown, it includes a box body 1 and a box cover 2; a battery is arranged in the box body 1; the box body 1 is bolted with two handles distributed on the left and right; the box body 1 is detachably connected to the box cover 2; It also includes a refrigerator 3, a cooling cylinder 4, a plug 5, a prompt plate 6 and a propulsion system; five refrigerators 3 in a linear array are bolted inside the box body 1; the box body 1 is connected to five cooling cylinders 4 in a linear array, and an insulation layer is provided on the outside of the cooling cylinder 4. The refrigerator 3 and the cooling cylinder 4 are used together to cool the test tube; the box body 1 is connected to a propulsion system; the propulsion system is connected to five plugs 5 in a linear array, and all the plugs 5 are connected to the battery in the box body 1 through cables, and the plugs 5 are used to power the refrigerator 3; the propulsion system is connected to five prompt plates 6 in a linear array, and the prompt plates 6 are slidably connected to the box body 1.

[0019] The pushing system includes a sliding plate 111, a spring member 112, a wedge block 113, a sliding plate 2 114, a spring member 2 115, a wedge block 2 116, a sliding block 117, a trapezoidal block 118, a flexible plate 119, a winding assembly and a pulling assembly; the box body 1 is slidably connected to five linear array sliding plates 111, and each sliding plate 111 is fixedly connected to the corresponding plug 5; each sliding plate 111 is fixedly connected to two spring members 112, and all spring members 112 are fixedly connected to the box body 1; each sliding plate 111 is fixedly connected to a wedge block 113; the box body 1 is slidably connected to five linear array sliding plates 2 114, and each sliding plate 2 114 is fixedly connected to the corresponding prompt plate 6 fixedly connected; each sliding plate 2 114 is fixedly connected to two spring members 2 115, and all spring members 2 115 are fixedly connected to the box body 1; each sliding plate 2 114 is fixedly connected to a wedge block 2 116 of the same shape and size as the wedge block 113; the box body 1 is slidably connected to a sliding block 117; the sliding block 117 is fixedly connected to a trapezoidal block 118 that matches the wedge block 113 and the wedge block 2 116; the box body 1 is connected to a winding assembly; the winding assembly is connected to a flexible plate 119 for keeping the wedge block 113 and the wedge block 2 116 in a pushed state, and the flexible plate 119 is fixedly connected to the sliding block 117; the box body 1 is connected to a pulling assembly, and the pulling assembly is connected to the sliding block 117.

[0020] The winding assembly includes a mounting circular plate 121, a winding disk 122 and a spring spring 123; the box body 1 is bolted to the mounting circular plate 121; the mounting circular plate 121 is rotatably connected to the winding disk 122 for winding up the flexible plate 119, and the flexible plate 119 is wound on the winding disk 122; the mounting circular plate 121 is fixedly connected to the spring spring 123, and the spring spring 123 is fixedly connected to the winding disk 122.

[0021] The pulling assembly includes a rotating rod 131, a winding disk 132, a rope 133 and a limiting unit; the box body 1 is rotatably connected to the rotating rod 131; the rotating rod 131 is slidingly connected to the winding disk 132 through a spline structure, and the winding disk 132 is rotatably connected to the box body 1; the winding disk 132 is fixedly connected to the rope 133 for pulling the sliding block 117 and the parts connected to it to move, and the rope 133 is fixedly connected to the sliding block 117; the box body 1 is connected to the limiting unit, and the limiting unit is connected to the rotating rod 131.

[0022] The limiting unit includes a round block 141, a rotating ring 142, a spring member 3 143, a support plate 144, a sliding rod 145, a spring member 4 146, a mounting ring 147 and a hemispherical block 148; the rotating rod 131 is fixedly connected to the round block 141; the round block 141 is rotatably connected to the rotating ring 142; the rotating ring 142 is fixedly connected to the spring member 3 143, and the spring member 3 143 is fixed to the box body 1; the box body 1 is bolted to the support plate 144; the support plate 144 penetrates and is slidably connected to four Slide rod 145; each slide rod 145 is fixed with a spring member 146, and all spring members 146 are fixed to the support plate 144; all slide rods 145 are fixed with a mounting ring 147; the mounting ring 147 is fixed with four hemispherical blocks 148; the circular block 141 is provided with four hemispherical grooves 14101, the hemispherical grooves 14101 match the shape of the hemispherical blocks 148, and the hemispherical grooves 14101 cooperate with the hemispherical blocks 148 to limit the rotation direction of the rotating rod 131.

[0023] The box body 1 is bolted with a limit plate 151 for enabling the flexible plate 119 to stably push the wedge block 113 and the wedge block 2 116 , and the limit plate 151 is slidably connected to the flexible plate 119 .

[0024] The specific operation of the above-mentioned embodiment 1 is as follows: It should be noted that, in this test kit, a test tube is placed in each cooling cylinder 4, and when taking out the test tube, the test tube in the cooling cylinder 4 can only be taken out from left to right in sequence. Conversely, when taking out the test tube loaded with the sample, the test tube loaded with the sample in the cooling cylinder 4 can only be taken out from right to left in sequence.

[0025] It should be noted in advance that, looking from top to bottom, the clockwise side of all hemispherical blocks 148 is an arc surface, while the counterclockwise side of all hemispherical blocks 148 is a flat surface, and the hemispherical groove 14101 matches the shape of the hemispherical block 148. Therefore, when the hemispherical groove 14101 is in contact with the hemispherical block 148, when the rotating rod 131 rotates counterclockwise, the hemispherical groove 14101 can push the hemispherical block 148, the mounting ring 147 and the sliding rod 145 to move downward, and compress the spring member 146 to disengage the hemispherical block 148 from the hemispherical groove 14101, that is, the rotating rod 131 can rotate counterclockwise normally, otherwise the rotating rod 131 will not be able to rotate clockwise.

[0026] When the medical staff needs to load the sample into the first cooling cylinder 4 from the left to the right, they first remove the box cover 2 from the box body 1 to open the box body 1, and then the medical staff takes out the test tube in the first cooling cylinder 4 from the left to the right, and loads the sample into the test tube, and then puts the test tube loaded with the sample back into the first cooling cylinder 4 from the left to the right.

[0027] Then, looking from top to bottom as a reference, the medical staff drives the rotating rod 131 to rotate counterclockwise. According to the aforementioned notes, the rotating rod 131 can rotate counterclockwise normally, so that the rotating rod 131 drives the winding disk 2 132 to rotate, so that the winding disk 2 132 reels the rope 133, so that the rope 133 pulls the sliding block 117 and the trapezoidal block 118 to move to the right, so that the trapezoidal block 118 passes between the first wedge block 113 and the wedge block 2 116 from left to right. When the trapezoidal block 118 moves to the right, the trapezoidal block 118 will drive the winding disk 1 122 to rotate through the flexible plate 119, so that the winding disk 1 122 unwinds the flexible plate 119. At this time, the clockwork spring 123 is in a stressed state, that is, the sliding block 117, the trapezoidal block 118 and the flexible plate 119 will move to the right together under the pull of the rope 133.

[0028] It should be noted that the following are the specific operations of the first refrigerator 3, cooling cylinder 4, plug 5, prompt plate 6, slide plate 111, spring member 112, wedge block 113, slide plate 2 114, spring member 2 115 and wedge block 2 116 from left to right. When the trapezoidal block 118 passes between the wedge block 113 and the wedge block 2 116, the trapezoidal block 118 will push the plug 5 away from the slide block 117 through the wedge block 113 and the slide plate 111 in turn, and compress the spring member 115. The spring member 112 allows the plug 5 to be inserted into the refrigerator 3, so that the battery in the box body 1 supplies power to the refrigerator 3 through the cable and the plug 5 in turn, so that the refrigerator 3 works, and the cooling surface of the refrigerator 3 cools the cooling cylinder 4 as a whole, so that the cooling cylinder 4 cools the sample loaded into the test tube therein to ensure the stability of the sample and the accuracy of the test results. At this time, the remaining plugs 5 will not be inserted into the corresponding refrigerator 3, that is, the refrigerator 3 corresponding to the cooling cylinder 4 without the sample loaded will not work.

[0029] Moreover, when the trapezoidal block 118 passes between the wedge block 113 and the wedge block 2 116, the trapezoidal block 118 will push the prompt plate 6 to move away from the slide block 117 through the wedge block 2 116 and the slide plate 2 114 in sequence, and compress the spring member 2 115, so that the prompt plate 6 is exposed to the outside of the box body 1, so that when subsequent medical personnel take out the test tubes in other cooling cylinders 4, they only need to use whether the prompt plate 6 is exposed to the outside of the box body 1 as a reference to determine whether the test tubes in the cooling cylinder 4 have been loaded with samples. At this time, the remaining prompt plates 6 are all retracted to the inside of the box body 1.

[0030] When the trapezoidal block 118 passes between the wedge block 113 and the wedge block 2 116 and is to the right of the wedge block 113 and the wedge block 2 116, the flexible plate 119 will contact the wedge block 113 and the wedge block 2 116 and press against the wedge block 113 and the wedge block 2 116, so that the wedge block 113 and the wedge block 2 116 remain in the pushed state, even if the plug 5 remains inserted into the refrigerator 3, the prompt plate 6 remains exposed to the outside of the box body 1. When the medical staff observes that the prompt plate 6 no longer moves, since the wedge block 113 and the wedge block 2 116 have the same shape and size, when the prompt plate 6 no longer moves, it means that the plug 5 has been fully inserted into the refrigerator 3, and the medical staff can stop driving the rotating rod 131 to rotate.

[0031] It should be noted that, with the view from top to bottom as the reference, since the reel 2 132 rotates counterclockwise when reeling in the rope 133, and according to the aforementioned notes, the rotating rod 131 cannot rotate clockwise, therefore, when the medical staff stops driving the rotating rod 131 to rotate, the clockwork spring 123 in a stressed state will not be able to drive the sliding block 117, the trapezoidal block 118 and the flexible plate 119 to move to the left due to the restriction of the rope 133, that is, when the medical staff stops driving the rotating rod 131 to rotate, the sliding block 117, the trapezoidal block 118 and the flexible plate 119 will remain stationary.

[0032] When the medical staff loads the samples into other cooling cylinders 4, they use the same method as above to insert the plug 5 corresponding to the cooling cylinder 4 containing the samples into the corresponding refrigerator 3, and expose the prompt plate 6 corresponding to the cooling cylinder 4 containing the samples to the outside of the box body 1 until all cooling cylinders 4 are filled with samples.

[0033] When the medical staff needs to take out the sample in the fifth cooling cylinder 4 from the left to the right, they first remove the box cover 2 from the box body 1 to open the box body 1, and then take out the test tube containing the sample in the fifth cooling cylinder 4 from the left to the right. Then, the medical staff pulls the round block 141 and the rotating ring 142 upward through the rotating rod 131 and compresses the spring member 3 143 to move the spherical groove 22202 away from the hemispherical block 148. At this time, due to the ball The second groove 22202 is no longer in contact with the hemispherical block 148, so the rotating rod 131 can rotate clockwise. Then, looking from the top down as a reference, the medical staff drives the second reel 132 to rotate clockwise a specified number of times through the rotating rod 131, so that the second reel 132 unwinds the rope 133. At this time, the spring spring 123 under stress will rewind the flexible plate 119, causing the flexible plate 119 to pull the sliding block 117 and the trapezoidal block 118 to move leftward. After the trapezoidal block 118 passes between the fifth wedge block 113 and the second wedge block 116 from the left to the right, it is located to the left between the wedge block 113 and the second wedge block 116. The following are the specific operations of the fifth refrigerator 3, cooling cylinder 4, plug 5, prompt plate 6, sliding plate 111, spring member 112, wedge block 113, sliding plate 2 114, spring member 2 115 and wedge block 2 116 from the left to the right. When the trapezoidal block 118 is to the left between the wedge block 113 and the second wedge block 116, the compressed spring member 112 will push the sliding plate 111, wedge block 113 and plug 5 to return to their initial positions, so that the plug 5 is pulled out of the refrigerator 3. Even if the plug 5 no longer provides power to the refrigerator 3, at this time, the remaining plugs 5 are still inserted into the corresponding refrigerator 3, that is, the refrigerator 3 corresponding to the cooling cylinder 4 where the sample has not been taken out will continue to be in working condition.

[0034] Moreover, when the trapezoidal block 118 is on the left between the wedge block 1 13 and the wedge block 2 116, the compressed spring member 2 115 will push the sliding plate 2 114, the wedge block 2 116 and the prompt plate 6 to return to their initial positions. Even if the prompt plate 6 is retracted to the inner side of the box body 1, when the medical staff observes that the prompt plate 6 no longer moves, the medical staff stops driving the rotating rod 131 to rotate and releases the rotating rod 131. At this time, the compressed spring member 3 143 will drive the rotating rod 131, the round block 141 and the rotating ring 142 to move downward and return to their initial positions, so that the spherical groove 2 22202 contacts the hemispherical block 148 again, so that the rotating rod 131 cannot rotate clockwise, so that the trapezoidal block 118 remains in its position on the left between the wedge block 1 13 and the wedge block 2 116.

[0035] Example 2 On the basis of Example 1, Figures 1-9As shown, a flexible tube 211 is also included; each cooling tube 4 is provided with a flexible tube 211, and the open end of each flexible tube 211 is wound around to the outside of the corresponding cooling tube 4 and fixed to the box body 1.

[0036] It also includes a fixed plate 221, a pull rod 222, a spring telescopic rod 223 and a ball 224; the box body 1 is slidingly connected to five fixed plates 221, and each fixed plate 221 is fixedly connected to the corresponding cooling cylinder 4, and the box body 1 and the cooling cylinder 4 are connected together through the fixed plate 221; the box body 1 is slidingly connected to five pull rods 222, and each pull rod 222 is fixedly connected to the corresponding fixed plate 221; the box body 1 is bolted to five spring telescopic rods 223; the telescopic part of each spring telescopic rod 223 is fixedly connected to a ball 224, and each ball 224 is in contact with the corresponding pull rod 222, and each pull rod 222 is provided with a spherical groove 22201, and each spherical groove 22201 is aligned with the corresponding ball 224.

[0037] Each cooling cylinder 4 is provided with an arc portion 401 for better pushing the flexible cylinder 211 to flip outward.

[0038] The specific operation of the above-mentioned embodiment 2 is as follows: Considering that when medical personnel place the test tube containing the sample into the corresponding cooling cylinder 4, since the cooling cylinder 4 is made of metal, when the test tube containing the sample falls into the inside of the cooling cylinder 4, the test tube containing the sample is easily broken, causing the sample in the test tube to overflow.

[0039] Therefore, by arranging a flexible cylinder 211 in the cooling cylinder 4, the test tube containing the sample is buffered by the flexible cylinder 211 to prevent the test tube containing the sample from being easily broken when falling into the cooling cylinder 4, causing the sample in the test tube to overflow.

[0040] Furthermore, it is considered that when medical personnel remove the test tube from the flexible tube 211 , due to the small diameter of the flexible tube 211 , the medical personnel are restricted by the flexible tube 211 and find it difficult to quickly remove the test tube from the flexible tube 211 .

[0041] Therefore, when the medical staff takes the test tube out of the flexible cylinder 211, they first pull the rod 222 upwards, so that the rod 222 pulls the cooling cylinder 4 upwards through the fixed plate 221. In this process, since the ball 224 is initially in contact with the rod 222, the telescopic portion of the spring telescopic rod 223 is initially in a compressed state. The rod 222 keeps the telescopic portion of the spring telescopic rod 223 in a compressed state through the ball 224. Therefore, when the spherical groove 222 is When the ball 224 passes through the ball 224, the telescopic portion of the spring telescopic rod 223 will push the ball 224 into the spherical groove 22201. When the ball 224 is stuck in the spherical groove 22201, the medical staff will feel an obvious jam when pulling the pull rod 222. When the medical staff feels the jam, they stop pulling the pull rod 222. At this time, the pull rod 222 will remain as is under the action of the spherical groove 22201, the spring telescopic rod 223 and the ball 224. Figure 9 The state shown, and, as Figure 6 As shown Figure 9 As shown, since the initial open end of the flexible cylinder 211 is wound around to the outside of the corresponding cooling cylinder 4 and is fixed to the box body 1, as the cooling cylinder 4 moves upward, the inner side of the flexible cylinder 211 will continue to flip outward, so that the part of the flexible cylinder 211 that is wound around to the outside of the corresponding cooling cylinder 4 will continue to increase, and conversely, the part of the flexible cylinder 211 on the inside of the cooling cylinder 4 will continue to decrease, so that the flexible cylinder 211 pushes the test tube to move upward, so that the upper end of the test tube is exposed to the upper side of the flexible cylinder 211, so that medical staff can better pick it up, to prevent the problem that due to the small diameter of the flexible cylinder 211, medical staff are restricted by the flexible cylinder 211 and find it difficult to quickly remove the test tube from the flexible cylinder 211.

[0042] When the test tube containing the sample needs to be placed in the flexible cylinder 211, the medical staff first pushes the pull rod 222 to move downward, so that the pull rod 222 pushes the cooling cylinder 4 downward through the fixed plate 221, and the cooling cylinder 4 returns to its initial position. In this process, the ball 224 is squeezed by the spherical groove 22201 to move in the direction away from the pull rod 222, and compresses the telescopic part of the spring telescopic rod 223, so that the fixation of the ball 224 to the pull rod 222 through the spherical groove 22201 will be released. Therefore, the pull rod 222 can move downward normally. When the cooling cylinder 4 returns to its initial position, the medical staff puts the test tube containing the sample into the flexible cylinder 211, and pushes the flexible cylinder 211 by the test tube containing the sample. Figure 9 The status shown is restored to Figure 6 The state shown in FIG. 1 is the state in which the test tube containing the sample is placed.

[0043] Example 3 On the basis of Example 2, Figures 1-10As shown, each pull rod 222 is provided with a second spherical groove 22202, each second spherical groove 22202 is aligned with the corresponding ball 224, and the distance between the second spherical groove 22202 and the ball 224 is set to be equal to the height of the flexible tube 211. This setting is used to facilitate manual disinfection of the flexible tube 211.

[0044] The specific operation of the above-mentioned embodiment 3 is as follows: Considering that before the second use of the present test kit, in order to prevent contaminants from easily remaining on the inner side of the flexible tube 211 after the present test kit is used, and then when the present test kit is used a second time, the contaminants remaining on the inner side of the flexible tube 211 will contaminate the sample in the new test tube, therefore, before the second use of the present test kit, medical personnel are required to disinfect the flexible tube 211. However, due to the small diameter and large height of the flexible tube 211, it is difficult for medical personnel to quickly disinfect the flexible tube 211.

[0045] Therefore, when the medical staff disinfects the flexible cylinder 211, the medical staff first pulls the pull rod 222 to move upward, so that the pull rod 222 pulls the cooling cylinder 4 upward through the fixed plate 221. In this process, when the spherical groove 22201 passes the ball 224, it can be seen from the process of the ball 224 being stuck in the spherical groove 22201 in Example 2 that the medical staff will feel the first obvious jam in the process of pulling the pull rod 222. After feeling the jam once, the medical staff continues to pull the pull rod 222 to move upward. In this process, the ball 224 will be squeezed away from the spherical groove 22201. The pull rod 222 moves in the direction of the pull rod 222 and compresses the telescopic part of the spring telescopic rod 223, that is, the pull rod 222 will continue to move, and as the pull rod 222 continues to move upward, when the spherical groove 22202 passes the ball 224, the telescopic part of the spring telescopic rod 223 will push the ball 224 to be stuck in the spherical groove 22202, so that the medical staff will feel a second obvious jam in the process of pulling the pull rod 222. When the medical staff feels the second jam, they stop pulling the pull rod 222. At this time, the pull rod 222 will remain as shown under the action of the spherical groove 22202, the spring telescopic rod 223 and the ball 224. Figure 10 The state shown, at the same time, as Figure 6 As shown Figure 10 As shown, since the initial open end of the flexible tube 211 is wound around to the outside of the corresponding cooling tube 4 and is fixed to the box body 1, and the distance between the spherical groove 22202 and the ball 224 is set to be equal to the height of the flexible tube 211, then at this time, the flexible tube 211 is pushed by the cooling tube 4, and the inner side of the flexible tube 211 will flip to the outside, allowing medical personnel to better disinfect the inner side of the flexible tube 211 to prevent the problem that the diameter of the flexible tube 211 is small and the height is large, which makes it difficult for medical personnel to quickly disinfect the flexible tube 211.

[0046] The above is a detailed introduction to the present application. Specific examples are used herein to illustrate the principles and implementation methods of the present application. The description of the above embodiments is only used to help understand the method and core idea of ​​the present application. At the same time, for those skilled in the art, based on the idea of ​​the present application, there may be changes in the specific implementation methods and application scope. In summary, the content of this specification should not be understood as a limitation on the present application.

Claims

1. A kit for rapid detection of HPV by isothermal amplification, comprising a box body (1) and a box cover (2); a battery is arranged in the box body (1); the box body (1) is detachably connected to the box cover (2); the characteristics are: The invention also comprises a refrigerator (3), a cooling cylinder (4), a plug (5), a prompt plate (6) and a driving system; a plurality of refrigerators (3) are fixedly connected in the box body (1); the box body (1) is connected to cooling cylinders (4) whose number matches that of the refrigerators (3), and a heat-insulating layer is arranged on the outside of the cooling cylinders (4); the box body (1) is connected to the driving system; the driving system is connected to plugs (5) whose number matches that of the refrigerators (3), and all the plugs (5) are connected to the battery in the box body (1) through cables; the driving system is connected to prompt plates (6) whose number matches that of the refrigerators (3), and the prompt plates (6) are slidably connected to the box body (1); the driving system is used to push the plugs (5) to be inserted into the refrigerator (3), and to push the prompt plates (6) to move and be exposed outside the box body (1).

2. A kit for rapid detection of HPV by isothermal amplification according to claim 1, characterized in that: The pushing system comprises a sliding plate 1 (111), a spring member 1 (112), a wedge block 1 (113), a sliding plate 2 (114), a spring member 2 (115), a wedge block 2 (116), a sliding block (117), a trapezoidal block (118), a flexible plate (119), a winding assembly and a pulling assembly; the box body (1) is slidably connected to sliding plates 1 (111) matching the number of plugs (5), and each sliding plate 1 (111) is fixedly connected to a corresponding plug (5); each sliding plate 1 (111) is fixedly connected to at least one spring member 1 (112), and all spring members 1 (112) are fixedly connected to the box body (1); each sliding plate 1 (111) is fixedly connected to a wedge block 1 (113); the box body (1) is slidably connected to sliding plates 2 (114) matching the number of prompt plates (6), and each sliding plate 2 (114) 114) are fixedly connected to the corresponding prompt plate (6); each sliding plate (114) is fixedly connected to at least one spring member (115), and all spring members (115) are fixedly connected to the box body (1); each sliding plate (114) is fixedly connected to a wedge block (116); the box body (1) is slidably connected to a sliding block (117); the sliding block (117) is fixedly connected to a trapezoidal block (118) matching the wedge block (113) and the wedge block (116); the box body (1) is connected to a winding assembly; the winding assembly is connected to a flexible plate (119), and the flexible plate (119) is fixedly connected to the sliding block (117); the winding assembly is used to wind up the flexible plate (119); the box body (1) is connected to a pulling assembly used to pull the sliding block (117) and parts connected thereto to move, and the pulling assembly is connected to the sliding block (117).

3. A kit for rapid detection of HPV by isothermal amplification according to claim 2, characterized in that: The winding assembly comprises a mounting circular plate (121), a winding disk (122) and a spring spring (123); the box body (1) is fixedly connected to the mounting circular plate (121); the mounting circular plate (121) is rotatably connected to the winding disk (122), and the flexible plate (119) is wound on the winding disk (122); the mounting circular plate (121) is fixedly connected to the spring spring (123), and the spring spring (123) is fixedly connected to the winding disk (122).

4. A kit for rapid detection of HPV by isothermal amplification according to claim 3, characterized in that: The pulling assembly comprises a rotating rod (131), a second winding disk (132), a rope (133) and a limiting unit; the box body (1) is rotatably connected to the rotating rod (131); the rotating rod (131) is slidably connected to the second winding disk (132) via a spline structure, and the second winding disk (132) is rotatably connected to the box body (1); the second winding disk (132) is fixedly connected to the rope (133), and the rope (133) is fixedly connected to the sliding block (117); the box body (1) is connected to a limiting unit for limiting the rotation direction of the rotating rod (131), and the limiting unit is connected to the rotating rod (131).

5. A kit for rapid detection of HPV by isothermal amplification according to claim 4, characterized in that: The limiting unit comprises a round block (141), a rotating ring (142), a spring member three (143), a support plate (144), a sliding rod (145), a spring member four (146), a mounting ring (147) and a hemispherical block (148); the rotating rod (131) is fixedly connected to the round block (141); the round block (141) is rotatably connected to the rotating ring (142); the rotating ring (142) is fixedly connected to the spring member three (143), and the spring member three (143) is fixedly connected to the box body (1); the box body (1) is fixedly connected to the support plate (144); the support plate (147) 144) is penetrated and slidably connected with a plurality of slide bars (145); each slide bar (145) is fixedly connected to a spring member four (146), and all spring members four (146) are fixedly connected to the support plate (144); all slide bars (145) are commonly fixedly connected to a mounting ring (147); the mounting ring (147) is fixedly connected to a plurality of hemispherical blocks (148); the hemispherical block (141) is provided with hemispherical grooves (14101) matching the number of the hemispherical blocks (148), and the hemispherical grooves (14101) match the shape of the hemispherical blocks (148).

6. A kit for rapid detection of HPV by isothermal amplification according to claim 5, characterized in that: It also includes a limiting plate (151); the box body (1) is fixedly connected to the limiting plate (151), and the limiting plate (151) is slidably connected to the flexible plate (119).

7. A kit for rapid detection of HPV by isothermal amplification according to any one of claims 5-6, characterized in that: It also includes a flexible tube (211); each cooling tube (4) is provided with a flexible tube (211) for protecting the test tube, and the open end of each flexible tube (211) is wound around to the outside of the corresponding cooling tube (4) and is fixedly connected to the box body (1).

8. The isothermal amplification rapid detection kit for HPV according to claim 7, characterized in that: The box body (1) also includes a fixing plate (221), a pull rod (222), a spring telescopic rod (223) and a sphere (224); the box body (1) is slidably connected to a number of fixing plates (221) that matches the number of cooling cylinders (4), and each fixing plate (221) is fixedly connected to a corresponding cooling cylinder (4), and the box body (1) and the cooling cylinder (4) are connected together via the fixing plate (221); the box body (1) is slidably connected to a number of pull rods (222) that matches the number of fixing plates (221), and each fixing plate (221) is fixedly connected to a corresponding cooling cylinder (4), and the box body (1) and the cooling cylinder (4) are connected together via the fixing plate (221); Each pull rod (222) is fixedly connected to a corresponding fixed plate (221); the box body (1) is fixedly connected with spring telescopic rods (223) whose number matches the pull rods (222); the telescopic portion of each spring telescopic rod (223) is fixedly connected with a ball (224), and each ball (224) is in contact with the corresponding pull rod (222); each pull rod (222) is provided with a spherical groove (22201), and each spherical groove (22201) is aligned with the corresponding ball (224).

9. A kit for rapid detection of HPV by isothermal amplification according to claim 8, characterized in that: Each cooling cylinder (4) is provided with an arc portion (401).

10. The isothermal amplification rapid detection kit for HPV according to claim 8, characterized in that: Each pull rod (222) is provided with a second spherical groove (22202), each second spherical groove (22202) is aligned with a corresponding round ball (224), and the distance between the second spherical groove (22202) and the round ball (224) is set to be equal to the height of the flexible tube (211).

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