Immune cell culture perfusion device

Abstract

The invention relates to the technical field of immune cell culture, and discloses an immune cell culture perfusion device which comprises a base, a base arranged on the base, a drainage component arranged in the base, an atomization component arranged on the base, a lifting component arranged on the base and a protection component arranged on the atomization component. Fresh culture media are atomized through the atomizer and introduced into the culture tank, the surface area of the culture media added into the culture tank is increased, the contact efficiency of immune cells in the culture tank and the culture media is improved, meanwhile, attachment points are provided for mist particles in the atomization tank through the capture rod and the supporting rod, and the atomization effect is improved. The fog particles are condensed into a liquid state and continuously dripped into the culture tank, so that the situation that the immune cells are damaged and inactive and functional due to shearing force generated by flowing of a liquid culture medium and acting on the immune cells during culture medium perfusion is avoided, a stable and suitable growth environment is provided for the immune cells, and the immune cells are protected. And proliferation and differentiation of immune cells in the culture tank are improved.

Description

Technical Field

[0001] The invention relates to the technical field of immune cell culture, in particular to an immune cell culture perfusion device. Background Art

[0002] White blood cells are an important type of immune cell in the body, responsible for protecting the human body from infection and other foreign invading substances. The main function of white blood cells is to identify, locate, and destroy invading external pathogens such as microorganisms and viruses, and to clear damaged or abnormal cells in the human body. White blood cells play a very important role in the entire immune system.

[0003] In order to explore the mechanism of the disease, culturing white blood cells in vitro and introducing stimuli such as pathogens and chemicals can simulate the immune response process in the body, which helps medical staff understand the nature of the disease and formulate treatment plans for patients with the disease; Currently, when perfusion culture of leukocytes in vitro is performed, a first pump body is required to be connected to a culture medium storage tank and a culture dish through water pipes, and then a second pump body is connected to the culture dish and a waste liquid recovery tank through water pipes. The first pump body is used to pump fresh culture medium in the culture medium storage tank into the culture dish, and then the second pump body is used to pump waste liquid in the culture dish into the recovery tank. However, when the fresh culture medium enters the culture dish through the water pipe, since there is a certain flow rate of the culture medium during the circulation process, when the fresh culture medium enters the culture dish, the flow of the culture medium will generate fluid shear force, which causes the immune cells in the culture dish to change their cell morphology and rupture their cell membranes due to the shear force, thereby affecting the activity and function of the leukocytes in the culture dish that are subjected to the shear force. Summary of the invention

[0004] The purpose of the present invention is to provide an immune cell culture perfusion device to solve the problems mentioned in the above process.

[0005] To achieve the above object, the present invention provides the following technical solution: an immune cell culture perfusion device, comprising a base, a base arranged on the base, a drainage component arranged in the base, an atomization component arranged on the base, a lifting component arranged on the base, and a protective component arranged on the atomization component; The liquid discharge component is used to cultivate cells and discharge waste liquid mixed with cell metabolism, the lifting component is used to lift the atomization component, and the protective component is used to receive the culture medium discharged when the atomization component rises; The atomization component includes an atomization tank, an atomizer arranged on the atomization tank, a storage tank arranged on the atomizer, an atomization groove arranged in an array in the atomization tank, a baffle arranged in the atomization tank, a drip box arranged in the atomization tank, a capture rod arranged on the drip box, a support rod arranged in an array on the capture rod, a drainage sheet arranged in an array on the drip box, and a drainage needle arranged on the drainage sheet.

[0006] As a preferred solution of the immune cell culture perfusion device of the present invention, the drainage component includes a fixed plate, a retaining ring arranged on the fixed plate, and a movable plate arranged at the lower end of the fixed plate.

[0007] As a preferred embodiment of the immune cell culture perfusion device of the present invention, the drainage component further comprises a culture plate, a culture tank disposed on the culture plate, and a discharge port disposed in the culture tank.

[0008] As a preferred solution of the immune cell culture perfusion device of the present invention, the lifting component includes a fixed seat, a swing rod arranged on the fixed seat, and a moving rod arranged on the swing rod.

[0009] As a preferred solution of the immune cell culture perfusion device of the present invention, wherein: a connecting rod is arranged on the moving rod, a push rod is arranged on the connecting rod, an upper limit plate 1 is arranged on the push rod, and a limit plate 2 is arranged on the base.

[0010] As a preferred embodiment of the immune cell culture perfusion device of the present invention, it comprises: a driving plate arranged on the base, a driving block arranged on the driving plate, a piston cylinder arranged on the base, a movable plug arranged in the base, and a plug block arranged in the base.

[0011] As a preferred embodiment of the immune cell culture perfusion device of the present invention, the device comprises: an opening and closing plate disposed in the base, an opening and closing rod disposed on the opening and closing plate, and a contact plate disposed on the opening and closing rod.

[0012] As a preferred embodiment of the immune cell culture perfusion device of the present invention, the protective component includes a shell, a mounting ring arranged in the shell, and a hinge arranged on the mounting ring.

[0013] As a preferred embodiment of the immune cell culture perfusion device of the present invention, it comprises: a storage groove arranged on the hinge, a storage plate arranged on the hinge, a first push rod arranged in the storage plate, and a second push rod arranged in the hinge.

[0014] As a preferred solution of the immune cell culture perfusion device of the present invention, wherein: a rotating ring is arranged in the shell, a pull rod is arranged on the rotating ring, a guide rod is arranged on the rotating ring, and a guide plate is arranged on the base.

[0015] Compared with the prior art, the present invention has the following beneficial effects: 1. The fresh culture medium is atomized by the atomizer and passed into the culture tank, increasing the surface area of ​​the culture medium added to the culture tank, thereby improving the contact efficiency between the immune cells in the culture tank and the culture medium, and promoting the absorption effect of the nutrients in the culture medium by the immune cells. At the same time, the capture rod and the support rod provide attachment points for the mist particles in the atomization tank, so that the mist particles condense into liquid and continue to drip into the culture tank, thereby avoiding the flow of liquid culture medium during the perfusion of the culture medium to generate shear force and act on the immune cells, causing immune cell damage, loss of activity and function, providing a stable and suitable growth environment for the immune cells, and improving the proliferation and differentiation of the immune cells in the culture tank; 2. By setting a baffle at the notch of the atomizer slot, the high-speed mist particles ejected by the atomizer are intercepted, thereby preventing the high-speed mist particles from penetrating the cell membrane of immune cells, causing the immune cells to lose activity and function; 3. The drain port is initially blocked by a fixed ring. When the culture medium in the culture tank accumulates to a certain mass, the spring is deformed, causing the culture plate to move downward. At this time, the fixed ring no longer blocks the drain port, so that the culture medium mixed with cell metabolic waste in the culture tank is discharged through the drain port and collected in the recovery tank. At this time, the immune cells in the culture tank are suspended on the upper liquid surface of the culture medium, so that the fresh culture medium can first contact with the immune cells in the culture tank and then be discharged through the drain port, thereby optimizing the growth environment of the cells. 4. The guide rod slides along the spiral groove, so that the rotating plate drives multiple hinges to move away from or approach each other, thereby blocking the opening and closing grooves. When the atomizer tank rises, the hinges block the opening and closing grooves, thereby preventing the culture medium discharged from the atomizer tank from falling at a higher position relative to the culture plate, thereby hitting the immune cells in the culture tank and causing cell membrane damage; 5. The lifting component makes it easier to take and put the culture plate. When the culture plate is pushed out of the feed trough, the moving plate will contact the contact plate, causing the opening and closing plate to shift, so that the overlapping hole overlaps with the spray port, and the disinfectant in the second air cavity is sprayed out from the spray port, thereby disinfecting and sterilizing the internal environment of the feed trough, providing a good growth environment for the next culture of immune cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the overall structure of the immune cell culture perfusion device of the present invention.

[0017] Figure 2It is a schematic diagram of the structure of the explosion of the atomizing tank of the immune cell culture perfusion device of the present invention.

[0018] Figure 3 The immune cell culture perfusion device of the present invention Figure 2 Schematic diagram of the structure enlarged at point A in the middle.

[0019] Figure 4 The immune cell culture perfusion device of the present invention Figure 2 Schematic diagram of the enlarged structure at point B.

[0020] Figure 5 The immune cell culture perfusion device of the present invention Figure 4 Schematic diagram of the enlarged structure at point C in the middle.

[0021] Figure 6 The schematic diagram of the structure of the device is a partial half-section view of the immune cell culture perfusion drainage component of the present invention.

[0022] Figure 7 It is a schematic structural diagram of the lifting component of the immune cell culture perfusion device of the present invention.

[0023] Figure 8 The immune cell culture perfusion device of the present invention Figure 7 Schematic diagram of the structure enlarged at point D in the middle.

[0024] Fig. 9 It is a structural schematic diagram of a partial cross-section of the lifting component of the immune cell culture perfusion device of the present invention.

[0025] Fig.10 It is a schematic structural diagram of a partial explosion of a protective component of the immune cell culture perfusion device of the present invention.

[0026] Fig.11 It is a schematic diagram of the structure of the top rod 1 and the top rod 2 of the immune cell culture perfusion device of the present invention.

[0027] Fig.12 It is a schematic diagram of the structure of the guide plate of the immune cell culture perfusion device of the present invention.

[0028] In the figure: 1. base; 11. base; 2. liquid discharge component; 21. fixed plate; 22. retaining ring; 23. movable plate; 24. culture plate; 25. culture tank; 26. drain port; 3. Atomizing components; 31. Atomizing tank; 311. Atomizing tank; 32. Atomizer; 33. Storage tank; 34. Baffle; 35. Drip box; 36. Capture rod; 37. Support rod; 38. Drainage sheet; 39. Drainage needle; 4. Lifting components; 41. Fixed seat; 42. Rocker; 43. Moving rod; 44. Connecting rod; 45. Push rod; 451. Limiting plate 1; 46. Limiting plate 2; 47. Driving plate; 471. Driving block; 48. Piston cylinder; 49. Moving plug; 491. Plug block; 492. Opening and closing plate; 493. Opening and closing rod; 494. Contact plate; 5. Protective component; 51. Shell; 52. Mounting ring; 53. Hinge; 531. Storage slot; 54. Storage plate; 55. Push rod 1; 56. Push rod 2; 57. Rotating ring; 571. Guide rod; 58. Pull rod; 59. Guide plate. DETAILED DESCRIPTION

[0029] The features and exemplary embodiments of various aspects of the present invention will be described in detail below. In the detailed description below, many specific details are proposed to provide a comprehensive understanding of the present invention. However, it is obvious to those skilled in the art that the present invention can be implemented without the need for some of these specific details. The following description of the embodiments is only to provide a better understanding of the present invention by illustrating examples of the present invention. The present invention is by no means limited to any specific configuration and algorithm proposed below, but covers any modification, replacement and improvement of elements, parts and algorithms without departing from the spirit of the present invention. In the accompanying drawings and the following description, known structures and technologies are not shown to avoid unnecessary ambiguity to the present invention.

[0030] Example 1 Reference Figure 1-9 , which is the first embodiment of the present invention, provides an immune cell culture perfusion device, the immune cell culture perfusion device comprises a base 1, a collecting tank is arranged on the base 1, the opening end of the collecting tank passes through and extends to a side of the base 1 close to the base 11, a base 11 is arranged on the base 1, the base 11 is arranged on the upper surface of the base 1, a feed trough is opened on the base 11, a liquid discharge component 2 is arranged in the base 11, an atomizing component 3 is arranged on the base 11, a lifting component 4 is arranged on the base 11, and a protective component 5 is arranged on the atomizing component 3; The liquid discharge component 2 is used to cultivate cells and discharge waste liquid mixed with cell metabolism, the lifting component 4 is used to lift the atomization component 3, and the protection component 5 is used to receive the culture medium discharged when the atomization component 3 rises; The atomizing component 3 includes an atomizing tank 31, an atomizer 32 disposed on the atomizing tank 31, a storage tank 33 disposed on the atomizer 32, an atomizing groove 311 arrayed in the atomizing tank 31, a baffle 34 disposed in the atomizing tank 31, a drip box 35 disposed in the atomizing tank 31, a capture rod 36 disposed on the drip box 35, a support rod 37 arrayed on the capture rod 36, a drainage sheet 38 arrayed on the drip box 35, and a drainage needle 39 disposed on the drainage sheet 38. The atomizing tank 31 is located above the base 11. The atomizer 32 is fixedly installed at the bottom of the atomizer tank 31, the storage tank 33 is connected to the end of the atomizer 32 away from the atomizer tank 31 and is in communication with the inside thereof, the end of the atomizer tank 31 away from the base 11 is provided with a bracket, the bracket is connected to the storage tank 33, and is used to ensure the stability when the storage tank 33 is connected to the atomizer 32. The atomizer 32 preferably adopts an ultrasonic atomizer 32, the ultrasonic atomizer 32 generates high-frequency oscillation through transducer coupling, and generates ultrasonic waves by the chip, the ultrasonic waves use the culture medium as the medium, and then pass through the resonance under the water tank. The emission window atomizes the fresh culture medium in the storage tank 33 into fine mist particles, so that the liquid culture medium is converted into a vapor state to produce an atomization effect. The mist particle emission window of the atomizer 32 is connected to the inside of the atomization tank 31. The baffle 34 is in a continuous S-shaped structure. The baffle 34 is arranged in the atomization tank 31 and is located at the notch position of the atomization groove 311. The end of the atomization tank 31 close to the base 11 is provided with a drip groove array, and the drip box 35 is arranged in the drip groove. The capture rod 36 is arranged in the middle area of ​​the drip box 35 close to the end of the atomization tank 31. A guide groove 1 is arranged in an array on the outer side wall of 36, and the support rod 37 is arranged at the position of the guide groove 1. A guide groove 2 is arranged in an array on a side of the drip box 35 close to the capture rod 36, and the guide groove 2 is communicated with the guide groove 1. A drainage groove is arranged in an array on the drip box 35, and the drainage groove is communicated with the guide groove 2. The drainage plate 38 has a V-shaped structure, and the drainage plate 38 is arranged on a side of the drip box 35 away from the capture rod 36, and the drainage plate 38 is located at the notch position of the drainage groove. A through groove is arranged at the bend of the drainage plate 38, and the drainage needle 39 is arranged in the through groove.

[0031] The drainage component 2 includes a fixed plate 21, a retaining ring 22 arranged on the fixed plate 21, and a movable plate 23 arranged at the lower end of the fixed plate 21. A fixed groove is opened in the fixed plate 21, and the fixed ring is arranged at the notch position of the fixed groove. A mounting ring 52 is arranged at the bottom of the fixed groove. A spring 1 is connected to the bottom of the fixed groove. The spring 1 is located in the mounting ring 52. A drainage groove is arranged in an array on the fixed plate 21. The drainage groove passes through the fixed plate 21 and is communicated with the fixed groove. A collecting groove is opened on a side of the movable plate 23 close to the fixed plate 21. Initially, the drainage grooves are all located at the upper end of the collecting groove, and the collecting groove coincides with the opening end of the collecting tank at this time.

[0032] The drainage component 2 also includes a culture plate 24, a culture trough 25 arranged on the culture plate 24, and a discharge port 26 arranged in the culture trough 25. The culture plate 24 is a disc-shaped structure. The culture plate 24 is slidably arranged in the fixed trough, and the outer diameter of the culture plate 24 and the inner diameter of the fixed ring are adapted to each other. The discharge port 26 is arranged in an array on the side wall of the culture trough 25 away from the end of the trough 25. The end of the spring away from the fixed plate 21 is connected to a side of the culture plate 24 away from the culture trough 25.

[0033] During use, fresh culture medium is first poured into the storage tank 33, and then the storage tank 33 is connected to the start-up nebulizer 32. At this time, the open end of the storage tank 33 is inverted, and the bracket limits and supports the storage tank 33, so as to avoid shaking after the storage tank 33 is connected to the nebulizer 32, resulting in damage to the storage tank 33 and the nebulizer 32 and waste of fresh culture medium. Due to the gravity effect on the fresh culture medium in the storage tank 33, the fresh culture medium in the storage tank 33 will flow from the storage tank 33 to the atomizer 32, and the atomizer 32 will be started at the same time. At this time, the fresh culture medium flowing into the atomizer 32 is converted from liquid to tiny mist particles under the action of the atomizer 32 and enters the atomizer tank 31; Since there are high-speed mist particles in the mist particles entering the atomizing tank 31 from the atomizer 32, after the high-speed mist particles enter the atomizing tank 31, some of the high-speed mist particles will contact with the support rod 37 and the capture rod 36 and adhere to their surfaces, while the other part of the high-speed mist particles will contact with the baffle 34, and the baffle 34 will block the high-speed mist particles so that the high-speed mist particles adhere to the surface of the baffle 34. As the number of fresh culture medium mist particles attached to the surface of the baffle 34 becomes excessive and gradually condenses into liquid culture medium, the liquid culture medium will drip into the culture tank 25 through the atomizing groove 311, and the fresh culture medium mist particles attached to the inner wall of the atomizing tank 31 will also condense into liquid fresh culture medium and enter the culture tank 25 through the atomizing groove 311; As the fresh culture medium atomized into mist particles continues to enter the atomizing tank 31, the concentration of the fresh culture medium mist particles in the atomizing tank 31 continues to increase, thereby increasing the contact probability between the fresh culture medium mist particles in the atomizing tank 31 and the support rod 37, the capturing rod 36, and the baffle 34. Then, the fresh culture medium mist particles attached to the surface of the support rod 37 will gradually condense into droplets. Under the action of gravity, the droplets of fresh culture medium will flow along the guide groove 1 to the guide groove 2. The fresh culture medium mist particles attached to the surface of the capturing rod 36 will also gradually condense into droplets. Under the action of gravity, part of the droplets of fresh culture medium will flow along the guide groove 1 to the guide groove 2, and the other part of the droplets of fresh culture medium will flow along the capturing rod 36 toward the drip box 35, and finally enter the guide groove 2 through the drip box 35. The fresh culture medium condensed from mist into liquid in the second guide groove flows into the drainage groove. As the liquid fresh culture medium is affected by gravity, the liquid fresh culture medium will contact the drainage sheet 38. At this time, the liquid fresh culture medium will flow along the drainage sheet 38 to the through groove position and contact the drainage needle 39. At this time, the condensed fresh culture medium will gradually drip into the culture groove 25 on the culture plate 24 through the drainage needle 39. Among them, the fresh flat culture medium mist particles in the atomizing tank 31 and not in contact with the support rod 37, the capture rod 36, and the baffle 34 will be discharged from the atomizing tank 31 through the atomizing groove 311 and enter the culture tank 25. As the fresh culture medium continuously enters the culture tank 25 from the storage tank 33, the immune cells in the culture tank 25 will absorb the nutrients in the fresh culture medium. Among them, at the beginning, the culture plate 24 will be pushed up to a certain distance from the bottom of the fixed groove under the elastic force of the spring 1, and the discharge port 26 on the culture plate 24 will contact the fixed ring, and the fixed ring will block the discharge port 26. When fresh culture medium continuously enters the culture groove 25, the fresh culture medium in the culture groove 25 will continue to increase, causing the liquid level of the culture medium in the culture groove 25 to gradually rise. At this time, the immune cells in the culture groove 25 will be suspended on the upper surface of the culture medium. At the same time, as the mass of the culture medium in the culture groove 25 continues to increase, the spring 1 is deformed by the gravity of the culture medium in the culture groove 25. At this time, the culture plate 24 moves downward toward the bottom of the fixed groove, so that the discharge port 26 on the culture plate 24 is separated from the fixed ring. At this time, the culture medium below the liquid level of the culture medium in the culture groove 25 will be discharged through the discharge port 26 and enter the fixed groove. Since the fresh culture medium is continuously discharged from the atomizing tank 31 into the culture groove 25, the volume of the culture medium in the culture groove 25 reaches a balanced state, so that the spring 1 is continuously in a deformed state; The culture medium entering the fixed tank enters the collecting tank through the drainage tank, and finally enters the collecting tank from the collecting tank, so that the waste liquid of the culture medium entering the collecting tank is recovered.

[0034] Example 2 Reference Figure 1-9 , which is the second embodiment of the present invention, and this embodiment is different from the first embodiment in that: The lifting component 4 includes a fixed seat 41, a rocker arm 42 arranged on the fixed seat 41, and a moving rod 43 arranged on the rocker arm 42. The fixed seat 41 is fixedly arranged on the upper surface of the base 1, and a support plate is symmetrically arranged on the outer side of the atomizer tank 31. Two hydraulic rods are arranged on the upper surface of the base 1, and the two hydraulic rods are symmetrically arranged on both sides of the atomizer tank 31, and the telescopic ends of the hydraulic rods are connected to the support plate. A hinge seat is arranged on the outer side wall of the atomizer tank 31, one end of the rocker arm 42 is hingedly matched with the hinge seat, and one end of the moving rod 43 is hingedly matched with one end of the rocker arm 42 away from the hinge seat. A key slot is opened in the middle area of ​​the rocker arm 42, and a sliding shaft is arranged on the fixed seat 41, and the sliding shaft is slidably arranged in the key slot.

[0035] A connecting rod 44 is arranged on the moving rod 43, a push rod 45 is arranged on the connecting rod 44, an upper limit plate 451 is arranged on the push rod 45, and a limit plate 46 is arranged on the base 11. One end of the connecting rod 44 is hingedly cooperated with the moving rod 43 away from the end thereof hinged with the rocker arm 42, and the limit plate 451 is arranged on the lower surface of the end of the connecting rod 44 close to the moving rod 43, and the limit plate 451 extends to the lower area of ​​the moving rod 43, the end of the connecting rod 44 away from the end thereof hinged with the moving rod 43 is hingedly cooperated with the push rod 45, the end of the push rod 45 away from the end thereof hinged with the connecting rod 44 is connected to the moving plate 23, and the limit plate 451 is located in the lower area of ​​the connecting rod 44.

[0036] A driving plate 47 is arranged on the base 1, a driving block 471 is arranged on the driving plate 47, a piston cylinder 48 is arranged on the base 1, a moving plug 49 is arranged in the base 11, and a plug block 491 is arranged in the base 11. The upper surface of the base 1 is symmetrically provided with a linear track, the driving plate 47 is slidably arranged on the linear track, the driving plate 47 is provided with a slide groove, the driving block 471 is slidably arranged in the slide groove, the slider is hinged with one end of the moving rod 43 close to the rocker 42, the piston cylinder 48 is symmetrically provided on the upper surface of the base 1, and a piston is provided on the driving plate 47. Rod, one end of the piston rod away from the driving plate 47 is slidably set in the piston cylinder 48, the end of the piston cylinder 48 away from the driving plate 47 is connected with an air guide tube, the base 11 is symmetrically provided with air cavity one and air cavity two, air cavity one is connected with the inside of air cavity two, one end of the air guide tube away from the piston cylinder 48 is connected to the base 11 and is connected with air cavity one, the movable plug 49 is slidably set in the air cavity one, and a connecting rod is set on a side of the movable plug 49 close to the air cavity two, the end of the connecting rod away from the movable plug 49 is connected to the plug block 491, and the plug block 491 is slidably set in the air cavity two.

[0037] An opening and closing plate 492 is arranged in the base 11, an opening and closing rod 493 is arranged on the opening and closing plate 492, and a contact plate 494 is arranged on the opening and closing rod 493. A plurality of spraying ports are symmetrically arranged on the inner wall of the feed trough, and the spraying ports are connected with the interior of the second air cavity. The opening and closing plate 492 is slidably arranged in the second air cavity, and an array of overlapping holes is arranged on the opening and closing plate 492. Initially, the overlapping holes and the spraying ports are in a mutually staggered position relationship. Contact grooves are symmetrically arranged on the inner wall of the feed trough, and the contact grooves are connected with the interior of the second air cavity. The opening and closing rod 493 is slidably arranged in the contact groove, and the contact plate 494 is arranged at the end of the opening and closing rod 493 away from the opening and closing plate 492. A spring piece 1 is arranged in the contact groove, and the end of the spring piece 1 away from the contact groove is connected to the opening and closing rod 493. A liquid inlet groove is symmetrically arranged in the base 11, and the liquid inlet groove is connected with the middle area of ​​the second air cavity. A disinfection tank is connected to the base 11, and disinfectant is filled in the disinfection tank, and the liquid inlet groove is connected with the interior of the disinfection tank.

[0038] During use, when the fixing plate 21 needs to be taken out, the hydraulic rod is started so that the telescopic end of the hydraulic rod pushes the support plate, so that the support plate lifts the atomizing tank 31 as a whole in a direction away from the base 1, and the protective component 5 moves synchronously with the atomizing tank 31; At the same time, the hinge seat on the atomizer tank 31 will pull the swing rod 42, so that the sliding shaft slides along the key slot, and then the swing rod 42 pulls the moving rod 43, so that the moving rod 43 pushes the connecting rod 44, and the connecting rod 44 pushes the push rod 45, so that the push rod 45 pushes the moving plate 23 out of the base 11 along the feed trough, wherein the second limit plate 46 will limit the moving plate 23, and the first limit plate 451 will limit the movement of the connecting rod 44, so that the push rod 45 can push the moving plate 23 out of the base 11; During the movement of the moving rod 43, the driving block 471 will slide along the slide groove and the driving plate 47 will move along the linear track toward the direction of the piston cylinder 48, so that the piston rod is subjected to the pushing force of the driving plate 47 to compress the gas in the piston cylinder 48 and push the gas from the piston cylinder 48 to the air chamber 1. At this time, the moving plug 49 in the air chamber 1 will slide in the air chamber 1, and the plug block 491 will move synchronously with the moving plug 49. Initially, the plug block 491 is located at one end of the air chamber 2 close to the air chamber 1, and the plug block 491 does not overlap with the notch of the liquid inlet groove. The disinfectant in the disinfection tank can enter the air chamber 2 through the liquid inlet groove. The plug block 491 will block the liquid inlet groove during its movement. At this time, the continued displacement of the plug block 491 will pressurize the disinfectant in the air chamber 2. At the same time, when the push rod 45 pushes the movable plate 23, the fixed plate 21 will move synchronously with the movable plate 23. When the movable plate 23 drives the fixed plate 21 to move to contact with the contact plate 494, the contact plate 494, the opening and closing rod 493 and the opening and closing plate 492 will move synchronously with the movable plate 23, and the spring sheet 1 will be deformed. At the same time, the overlap hole on the opening and closing plate 492 will overlap with the spraying port. At this time, the disinfectant in the air cavity 2 will pass through the overlap hole and be sprayed out from the spraying port under the action of pressure. At the same time, the push rod 45 has pushed the movable plate 23 and the fixed plate 21 out of the feed chute, and the fixed plate 21 on the movable plate 23 can be removed at this time. When the immune cells need to be cultured again, after the immune cells are placed in the culture tank 25, the fixed plate 21 is placed on the movable plate 23 that has extended out of the feed tank, and then the hydraulic rod is started to shrink the piston end of the hydraulic rod. At this time, the push rod 45 will pull the movable plate 23 and the fixed plate 21, so that the fixed plate 21 is shifted to the reduction tank. At the same time, the spring piece 1 is reset, the overlapping hole and the spray port are misaligned, the atomizing tank 31 is reset, the piston rod is reset, the movable plug 49 is reset, and the plug block 491 is reset. When the plug block 491 does not block the liquid inlet tank, the disinfectant in the disinfection tank will enter the air cavity 2 through the liquid inlet tank.

[0039] The remaining structures are the same as those of Example 1.

[0040] Example 3 Reference Figure 1-12 , which is the third embodiment of the present invention, and this embodiment is different from the second embodiment in that: The protective component 5 includes a shell 51, a mounting ring 52 arranged in the shell 51, and a hinge 53 arranged on the mounting ring 52. A cavity is arranged in the shell 51, and an opening and closing groove is arranged through the shell 51. The mounting ring 52 is arranged on the inner wall of one side of the cavity inside the shell 51 and is located at the notch of the opening and closing groove. The hinge 53 has a petal-shaped structure, and one corner of the hinge 53 is hingedly matched with the mounting ring 52. A plurality of hinges 53 are arranged in an array on the mounting ring 52. The plurality of hinges 53 can be synchronously rotated at a certain angle to fit each other and block the opening and closing groove.

[0041] A storage groove 531 is arranged on the hinge 53, a storage plate 54 is arranged on the hinge 53, a push rod 1 55 is arranged in the storage plate 54, and a push rod 2 56 is arranged in the hinge 53. A notch is provided on the hinge 53, the storage plate 54 is hingedly arranged at the position of the notch, and a torsion spring is arranged at the hinge of the storage plate 54 and the notch, a top groove 1 is provided in the storage plate 54, a spring piece 2 is arranged in the top groove 1, one end of the spring piece 2 is connected to the bottom of the top groove 1, and the other end is connected to the push rod 1 55, the push rod 1 55 is slidably arranged in the top groove 1, a top groove 2 is provided in the hinge 53, an elastic groove is provided in the top groove 2, the push rod 2 56 is slidably arranged in the top groove 2, a spring 2 is sleeved on the outside of the push rod 2 56, the spring 2 is arranged in the elastic groove, one end of the spring 2 is connected to the side wall of the elastic groove, and the other end is connected to the push rod 2 56, and the end of the push rod 2 56 away from the notch extends to the outside of the top groove 2.

[0042] A rotating ring 57 is arranged in the shell 51, a pull rod 58 is arranged on the rotating ring 57, a guide rod 571 is arranged on the rotating ring 57, and a guide plate 59 is arranged on the base 11. The rotating ring 57 is rotatably arranged in the shell 51, the pull rod 58 is hingedly arranged on the rotating ring 57, and a plurality of pull rods 58 are arranged in an array on the rotating ring 57, one end of the pull rod 58 away from the rotating ring 57 is hingedly matched with an end of the hinge 53 close to the notch, guide grooves are symmetrically opened on the shell 51, and the guide rod 571 penetrates and is slidably arranged in the guide grooves, and two guide plates 59 are symmetrically arranged on the bottom plate, and a spiral groove is opened on the guide plate 59, and the guide rod 571 is slidably arranged in the spiral groove.

[0043] During use, at the beginning, the storage plate 54 rotates to a position away from the gap under the elastic force of the torsion spring. When the atomizer tank 31 rises, the guide rod 571 will slide along the spiral groove. Under the guidance of the spiral groove, the rotating ring 57 will rotate, causing the multiple hinges 53 to approach each other under the pull of the pull rod 58. In the process of the multiple hinges 53 approaching each other and gradually fitting together, the adjacent hinges 53 will push each other and force the storage plate 54 to rotate to block the gap. When the top rod 1 55 on the storage plate 54 contacts the hinge 53, the spring piece 2 will be deformed, and the top rod 1 55 will shrink into the top groove 1. When the top rod 1 55 moves to overlap with the top groove 2, the top rod 1 55 will extend out and insert into the top groove 2 under the action of the spring piece 2, and the opening and closing groove is blocked at this time. The fresh culture medium discharged from the atomizer tank 31 will be blocked by the multiple hinges 53 and collected in the storage groove 531; When the atomizer tank 31 is in the process of descending, the guide rod 571 will reset along the spiral groove again. At this time, the rotating ring 57 will reset, and the multiple hinges 53 will gradually move away from each other. When the pull rod 58 pulls the hinge 53 to the initial position, the push rod 2 56 will contact the inner wall of the shell 51, and the spring 2 will deform. The push rod 2 56 moves toward the direction of the push rod 1 55, and then the push rod 2 56 will push the push rod 1 55 out of the top groove 2. At this time, since the storage plate 54 is not subject to the pushing force of the adjacent hinge 53, the storage plate 54 will reset under the elastic force of the torsion spring, causing the gap to open at this time, and then the culture medium collected in the storage groove 531 will enter the shell 51 through the gap for collection.

[0044] The remaining structure is the same as that of Example 2.

[0045] Different technical features appearing in different embodiments can be combined to achieve beneficial effects. Based on the study of the drawings, the specification and the claims, those skilled in the art should be able to understand and implement other variations of the disclosed embodiments. In the claims, the term "comprising" does not exclude other devices or steps; the indefinite article "a" does not exclude a plurality; the terms "first" and "second" are used to indicate names rather than to indicate any specific order. Any figure marks in the claims should not be construed as limiting the scope of protection. The functions of multiple parts appearing in the claims can be implemented by a single hardware or software module. The fact that certain technical features appear in different dependent claims does not mean that these technical features cannot be combined to achieve beneficial effects.

Claims

1. An immune cell culture perfusion device, comprising a base (1), characterized in that: A base (11) disposed on the base (1), a liquid discharge component (2) disposed in the base (11), an atomization component (3) disposed on the base (11), a lifting component (4) disposed on the base (11), and a protective component (5) disposed on the atomization component (3); The liquid discharge component (2) is used to cultivate cells and discharge waste liquid mixed with cell metabolism, the lifting component (4) is used to lift the atomization component (3), and the protective component (5) is used to receive the culture medium discharged when the atomization component (3) rises; The atomizing component (3) comprises an atomizing tank (31), an atomizer (32) arranged on the atomizing tank (31), a storage tank (33) arranged on the atomizer (32), an atomizing groove (311) arranged in an array in the atomizing tank (31), a blocking plate (34) arranged in the atomizing tank (31), a dripping box (35) arranged in the atomizing tank (31), a catching rod (36) arranged on the dripping box (35), a supporting rod (37) arranged in an array on the catching rod (36), a drainage plate (38) arranged in an array on the dripping box (35), and a drainage needle (39) arranged on the drainage plate (38).

2. The immune cell culture perfusion device according to claim 1, characterized in that: The liquid discharge component (2) comprises a fixed plate (21), a retaining ring (22) arranged on the fixed plate (21), and a movable plate (23) arranged at the lower end of the fixed plate (21).

3. The immune cell culture perfusion device according to claim 1, characterized in that: The drainage component (2) further comprises a culture plate (24), a culture tank (25) arranged on the culture plate (24), and a discharge port (26) arranged in the culture tank (25).

4. The immune cell culture perfusion device according to claim 1, characterized in that: The lifting component (4) comprises a fixed seat (41), a swing rod (42) arranged on the fixed seat (41), and a moving rod (43) arranged on the swing rod (42).

5. The immune cell culture perfusion device according to claim 4, characterized in that: A connecting rod (44) disposed on the moving rod (43), a push rod (45) disposed on the connecting rod (44), a first limit plate (451) disposed on the push rod (45), and a second limit plate (46) disposed on the base (11).

6. The immune cell culture perfusion device according to claim 1, characterized in that: A drive plate (47) disposed on the base (1), a drive block (471) disposed on the drive plate (47), a piston cylinder (48) disposed on the base (1), a movable plug (49) disposed in the base (11), and a plug block (491) disposed in the base (11).

7. The immune cell culture perfusion device according to claim 1, characterized in that: An opening and closing plate (492) is arranged in the base (11), an opening and closing rod (493) is arranged on the opening and closing plate (492), and a contact plate (494) is arranged on the opening and closing rod (493).

8. The immune cell culture perfusion device according to claim 1, characterized in that: The protective component (5) comprises a shell (51), a mounting ring (52) arranged in the shell (51), and a hinge (53) arranged on the mounting ring (52).

9. The immune cell culture perfusion device according to claim 8, characterized in that: A storage groove (531) is provided on the hinge (53), a storage plate (54) is provided on the hinge (53), a first push rod (55) is provided inside the storage plate (54), and a second push rod (56) is provided inside the hinge (53).

10. The immune cell culture perfusion device according to claim 9, characterized in that: A rotating ring (57) disposed in the housing (51), a pull rod (58) disposed on the rotating ring (57), a guide rod (571) disposed on the rotating ring (57), and a guide plate (59) disposed on the base (11).

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