Electric field in-situ regulation and control device suitable for single-molecule super-resolution fluorescence microscope

Abstract

The invention belongs to the technical field of photochemistry, and particularly relates to a photochemical analysis technology, in particular to an electric field in-situ regulation and control device suitable for a single-molecule super-resolution fluorescence microscope, which comprises a working electrode, a counter electrode and a microchannel, the two electrode plates are bonded through a double-faced adhesive tape etched with a micro-channel to form a sandwich structure; the electrode plate is further provided with a through hole and connected with the flow guide pipe, aiming at the urgent need of studying the adjusting effect of an electric field on the photocatalytic oxidation-reduction reaction, the electric field adjusting and controlling device for the single-molecule super-resolution fluorescence microscope makes up for the lack of an electric field adjusting and controlling device suitable for the single-molecule super-resolution fluorescence microscope, and has good practical application value.

Description

Technical Field

[0001] The present invention belongs to the field of energy photocatalysis and photochemical technology, and specifically relates to an electric field in-situ control device suitable for a single-molecule super-resolution fluorescence microscope and its application in a photocatalytic process. Background Art

[0002] The information disclosed in this background technology section is only intended to enhance the understanding of the overall background of the invention, and should not necessarily be regarded as an admission or any form of suggestion that the information constitutes the prior art already known to a person skilled in the art.

[0003] The efficient conversion of solar energy into hydrogen energy through water splitting reaction has been considered as one of the most promising ways to solve energy and environmental problems. However, due to the low separation of photogenerated carriers, the photocatalytic efficiency is still not ideal. In recent years, various strategies have been gradually developed, such as building an S-heterojunction, Z-heterojunction, homojunction and Schottky junction to establish a built-in electric field to achieve improved carrier separation efficiency. The application of external electric field in the photocatalytic process is a more flexible and controllable strategy, which has attracted more and more attention from researchers. However, the regulation of photocatalytic efficiency by external field is still unclear due to the existence of multiple processes in the photocatalytic process, 1) the generation of photogenerated carriers; 2) the separation and transfer of photogenerated carriers driven by external field; 3) the redox reaction induced by photogenerated carriers on the catalyst surface. In addition, the efficiency of photocatalytic redox reaction is affected by factors such as the adsorption of reactants on the catalyst surface, the catalytic conversion of reactants to products, and the desorption of products. These processes are not only spatiotemporally dynamic, but also involve surface reactions at the microscopic scale. To study the regulatory effect of electric field on photocatalytic redox reaction, it is necessary to explore real-time and in situ characterization techniques.

[0004] Single-molecule super-resolution fluorescence microscopy has high temporal and spatial resolution and can reveal the microscopic mechanism of photocatalysis. However, due to the lack of electric field control devices suitable for single-molecule super-resolution fluorescence microscopy, it is currently difficult to visualize the photocatalytic redox reaction on the surface of catalyst particles under different electric fields, and it is even more difficult to analyze the microscopic mechanism of electric field control by analyzing the single-molecule dynamics of the redox reaction under the action of an applied electric field. Summary of the invention

[0005] In view of the shortcomings of the above-mentioned prior art, the present invention provides an electric field control device suitable for a single-molecule super-resolution fluorescence microscope to realize the in-situ control analysis of the photocatalytic redox reaction on the surface of the catalyst particles in the actual photocatalytic process. The constructed electric field control device is mainly composed of an indium tin oxide (ITO) glass two-electrode system. ITO glass has excellent light transmittance and conductivity, which is conducive to the application of a photocatalytic light source and a fluorescent excitation light source and the collection of fluorescent signals. Between the parallel ITO glass electrode plates, due to the application of a bias voltage, an electric field perpendicular to the electrode plates and uniformly parallel will be formed, which promotes the separation of photogenerated carriers of the catalyst particles under the action of the Coulomb force and improves the efficiency of the photocatalytic reaction. Therefore, the electric field control device can simultaneously meet the photocatalytic reaction and fluorescence display monitoring; on the other hand, based on the electric field control device, the control process of photocatalytic redox under the electric field is monitored in situ at the single-molecule level, which helps to realize the visualization of the photocatalytic redox reaction on the surface of the catalyst particles under different electric fields, the single-molecule kinetic analysis of the redox reaction under the action of the applied electric field, and the analysis of the microscopic mechanism of electric field control, which has good practical application value.

[0006] In order to achieve the above technical objectives, the technical solution adopted by the present invention is as follows:

[0007] The first aspect of the present invention provides an electric field in situ regulation device suitable for single-molecule super-resolution fluorescence microscopy, comprising: a working electrode, a counter electrode, and a microchannel. The working electrode and the counter electrode are electrode plates arranged in parallel, and the two electrode plates are bonded by double-sided tape etched with microchannels to form a sandwich structure; the electrode plates are also provided with through holes and are connected to the flow guide tube.

[0008] In some embodiments, the working electrode and the counter electrode are both ITO glass electrodes;

[0009] In some embodiments, the ITO glass electrode has the following dimensions: a width of 2-3 cm, a length of 4-5 cm, and a thickness of 3-4 mm or 0.15-0.17 mm;

[0010] In some embodiments, the through hole has a diameter of 1-1.2 mm.

[0011] In some embodiments, the double-sided tape has a size of 2.0-2.1 cm×5.0-5.1 mm×150-160 μm.

[0012] The second aspect of the present invention provides the use of the above-mentioned electric field in situ control device suitable for single-molecule super-resolution fluorescence microscopy in single-molecule level quantitative detection in real-time in situ photocatalysis.

[0013] The third aspect of the present invention provides a method for constructing the above-mentioned electric field in situ control device suitable for single-molecule super-resolution fluorescence microscopy, comprising:

[0014] Clean the ITO glass electrode to meet the requirements of single-molecule imaging;

[0015] The two cleaned ITO glass electrodes were punched and incubated with catalyst particles, respectively serving as the upper electrode and the lower electrode of the electric field control device;

[0016] Two ITO glass electrodes are assembled into a sandwich structure by bonding them with double-sided tape etched with channels, and a flow guide tube is connected to the ITO glass electrode with holes so that the channels in the sandwich structure can be pumped with the reaction solution.

[0017] In some embodiments, the specific method of the cleaning treatment is: using glass cleaning agent and alcohol to repeatedly ultrasonically clean for 20-30 minutes, finally rinsing with water, blowing dry with nitrogen, placing on a clean bench, and sealing for storage;

[0018] In some embodiments, in the catalyst incubation treatment of the ITO glass electrode, the ITO glass electrode used has a thickness of 0.15-0.17 mm, and the concentration of the catalyst particle aqueous solution is 3-4 mg / mL.

[0019] In some embodiments, the reaction solution includes: an oxidation reaction solution and a reduction reaction solution, more specifically, a photogenerated electron scavenger MV in an AR solution, 2+ And CH3OH, the photogenerated hole scavenger in Re solution.

[0020] A fourth aspect of the present invention provides the use of the above-mentioned electric field control device suitable for single-molecule super-resolution fluorescence microscopy in controlling the photocatalytic redox reaction occurring on the surface of BiVO4 photocatalyst particles.

[0021] In some embodiments, detection and analysis are performed by single-molecule level imaging under different electric fields to obtain a super-resolution spatial distribution of the photocatalytic redox reaction occurring on the surface of the BiVO4 photocatalyst particles.

[0022] In some embodiments, the electric field control device is used to analyze the activity of the photocatalytic redox reaction occurring on the surface of BiVO4 photocatalyst particles, and the frequency of the photocatalytic redox reaction occurring on the surface of BiVO4 photocatalyst particles under different electric fields is quantified; and the control pathway of the photocatalytic redox reaction occurring on the surface of BiVO4 photocatalyst particles under electric field control is analyzed by single molecule dynamics analysis.

[0023] The beneficial effects of the present invention are:

[0024] (1) Compared with the prior art, the present invention fills the gap in the electric field control device for single-molecule super-resolution fluorescence microscopy, making it possible to analyze the control process of the electric field during the photocatalytic reaction.

[0025] (2) The electric field control device constructed by the present invention is mainly composed of an indium tin oxide (ITO) glass two-electrode system. ITO glass has excellent light transmittance and conductivity, which is conducive to the application of photocatalytic light source and fluorescent excitation light source and the collection of fluorescent signals. Between the parallel ITO glass electrode plates, due to the application of bias voltage, an electric field perpendicular to the electrode plates and uniformly parallel will be formed, which promotes the separation of photogenerated carriers of catalyst particles under the action of Coulomb force and improves the efficiency of photocatalytic reaction. Therefore, the electric field control device can simultaneously meet the requirements of photocatalytic reaction and fluorescence display monitoring; on the other hand, based on the electric field control device, the control process of photocatalytic redox under electric field is monitored in situ at the single-molecule level, which helps to realize the visualization of photocatalytic redox reaction on the surface of catalyst particles under different electric fields, the single-molecule dynamics analysis of redox reaction under the action of external electric field and the analysis of the microscopic mechanism of electric field control, which has good practical application value.

[0026] (3) The operation method of the present application is simple, universal, and easy to scale up for production. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] The drawings in the specification, which constitute a part of the present application, are used to provide further understanding of the present application. The illustrative embodiments of the present application and their descriptions are used to explain the present application and do not constitute improper limitations on the present application.

[0028] Figure 1 This is a construction flow chart of the electric field control device suitable for single-molecule super-resolution fluorescence microscopy of the present invention; wherein, 1. ITO glass electrode, 2. double-sided tape, 3. through hole, 4. flow guide tube.

[0029] Figure 2 This is a physical picture of the electric field control device applicable to single-molecule super-resolution fluorescence microscopy of the present invention;

[0030] Figure 3 This is a single-molecule imaging diagram of the BiVO4 photocatalytic process of the present invention. A is a schematic diagram of the photochemical reaction under a total internal reflection fluorescence (TIRF) microscope. B is a bright field image of a single BiVO4. C is the original fluorescence signal of the photocatalytic oxidation product Rf. D is the original fluorescence signal of the photocatalytic oxidation product Rf in the red area (3 pixels × 3 pixels) in C. E is a high-resolution fluorescence image of D obtained using the centroid algorithm (each pixel size: 32.5nm × 32.5nm). F is the time trajectory of the fluorescence burst in the red area (3 pixels × 3 pixels) in C within 30s;

[0031] Figure 4 It is a single-component imaging diagram of the regulation and application of the present invention in the BiVO4 photocatalytic process. A is the frequency of the photocatalytic oxidation reaction of the {110} plane of the BiVO4 single crystal under different external electric fields. B is a thermal map of the frequency of the photocatalytic reduction reaction of the {010} plane of the BiVO4 single crystal under different external electric fields. C is the effect of the bond bending induced by the built-in electric field on the photogenerated redox reaction. D is the frequency of the photocatalytic redox reaction of the BiVO4 single crystal under different electric fields in A and B. E is the effect of the {010} plane bond bending (h2) and the {110} plane bond bending (h2') on the photocatalytic redox reaction under the action of the electric field. F is the photocurrent generated under the action of an external electric field;

[0032] Figure 5 This is a single-component kinetic analysis diagram of the application of the present invention in the BiVO4 photocatalytic process. A is the process of reactant absorption, catalytic conversion of reactants to products and product desorption. [S] is the concentration of the reactant (AR or Re). K1 is the adsorption equilibrium constant. k1 is the conversion rate constant. k2 is the indirect desorption rate constant, k2 is the reabsorption equilibrium constant, and k3 is the direct desorption rate constant. In the voltage range of -0.4V to 2.0V, B is k1 for oxidation and reduction reactions. C is K1 for oxidation and reduction reactions. D is k2, k2 and k3 for oxidation reactions. E is k2, k2 and k3 for reduction reactions. DETAILED DESCRIPTION

[0033] It should be noted that the following detailed descriptions are illustrative and are intended to provide further explanation of the present application. Unless otherwise specified, all technical and scientific terms used in the present application have the same meanings as those commonly understood by those skilled in the art to which the present application belongs.

[0034] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, it indicates the presence of features, steps, operations, devices, components and / or combinations thereof.

[0035] As introduced in the background technology, due to the lack of electric field control devices suitable for single-molecule super-resolution fluorescence microscopy, it is difficult for the present invention to realize the visualization of photocatalytic redox reactions on the surface of catalyst particles under different electric fields, and it is even more impossible to analyze the microscopic mechanism of electric field control by analyzing the single-molecule dynamics of redox reactions under the action of an external electric field.

[0036] In view of this, in a specific embodiment of the present invention, an electric field control device suitable for single-molecule super-resolution fluorescence microscopy is constructed. The method includes: cleaning of the ITO glass electrode 1; drilling of the ITO glass electrode 1; incubation of catalyst particles of the ITO glass electrode 1; assembly of the ITO glass electrode 1; and the electric field control device controls the photocatalytic redox reaction occurring on the surface of the BiVO4 photocatalyst particles.

[0037] In a first aspect of the present invention, an electric field control device suitable for single-molecule super-resolution fluorescence microscopy is constructed, and the method comprises:

[0038] --Cleaning of the ITO glass electrode 1: In order to reduce the spontaneous fluorescence of dust and organic pollutants under the excitation light source and the background fluorescence caused by scattered light, the ITO glass electrode 1 is firstly cleaned thoroughly.

[0039] The specific method for cleaning the ITO glass electrode 1 is as follows: the ITO glass electrode 1 is independently immersed in a glass cleaner and ultrasonically cleaned for three times for 20 minutes. After the ITO glass electrode 1 is ultrasonically cleaned with a glass cleaner, it is fully rinsed with deionized water and then independently immersed in anhydrous ethanol and ultrasonically cleaned for three times for 20 minutes. After the ITO glass electrode 1 is ultrasonically cleaned with anhydrous ethanol and ultrasonically cleaned with deionized water, it is placed on a clean bench rack, dried with nitrogen and sealed to prevent environmental dust contamination.

[0040] -- Punching of the ITO glass electrode 1: In order to form a flow channel for the component reaction solution, the 3 mm thick ITO glass electrode 1 is cleaned and then punched to form inlet / outlet holes (ie, through holes 3).

[0041] After the ITO glass electrode 1 is cleaned, it is placed on the table of a bench drill. The ITO glass electrode 1 is marked and punched at 2 cm intervals. The hole diameter is 2 mm.

[0042] --Deposition of catalyst particles on the ITO glass electrode 1: In order to realize the photocatalytic reaction, bismuth vanadate particles are in-situ deposited on the bottom of the fluid channel, that is, on the surface of the ITO glass electrode 1.

[0043] The 0.17 mm thick ITO glass electrode 1 was cleaned and placed in a clean glass dish, and 1 ml of 3 mg / mL catalyst particle aqueous solution was added. The ITO glass electrode 1 was placed in an oven at 90° C. in air overnight to solidify the catalyst particles.

[0044] --Assembly of ITO glass electrode 1: In order to realize the photocatalytic reaction, a micro reaction device is assembled from two ITO glass electrodes 1 and double-sided tape 2 etched with fluid channels.

[0045] The 0.17 mm thick ITO glass electrode 1 is used as the bottom layer, the double-sided adhesive tape 2 etched with the fluid channel is used as the middle layer, and the 3 mm thick ITO glass electrode 1 is used as the top layer to form a sandwich structure to form a miniature reaction device. The 3 mm thick ITO glass electrode 1 is bonded with inlet / outlet conduits (i.e., flow guide tubes 4) at the punched holes to facilitate the flowing reaction solution to enter the reaction channel and flow out of the reaction channel. The ITO glass electrode 1 is bonded with electrode clips on the ITO coating surface to facilitate the constant voltage power supply to apply bias voltage.

[0046] In the second aspect of the present invention, the above-mentioned electric field control device is used to control the photocatalytic redox reaction occurring on the surface of BiVO4 photocatalyst particles. By detecting and analyzing under different electric fields at the single-molecule level imaging, the super-resolution spatial distribution changes and quantitative information of the reaction frequency of the photocatalytic redox reaction occurring on the surface of BiVO4 photocatalyst particles are obtained. The control pathway of the photocatalytic redox reaction occurring on the surface of BiVO4 photocatalyst particles by electric field control is analyzed by single-molecule dynamics analysis.

[0047] Among them, the bias voltage modulation range of the electric field control device is -5V to 5V. The photocatalytic redox reactions occurring on the surface of the BiVO4 photocatalyst particles are the oxidation reaction of Amplex Red (AR) and the reduction reaction of Resazurin (Re). The products of the photocatalytic redox reactions are all Resorufin (Rf). The product can be excited by a 525nm laser to produce a fluorescence signal with a maximum emission wavelength of 590nm. The present invention can use the frequency and spatial distribution of the fluorescent single molecule of the product to analyze the microscopic mechanism of the electric field regulation of the photocatalytic redox reaction. The single-molecule fluorescence signal generated by the photocatalytic redox reaction occurring on the surface of the BiVO4 photocatalyst particles is observed and analyzed by a self-built single-molecule super-resolution fluorescence microscopy system (patent CN202010115837.6).

[0048] The present invention is further explained by the following examples, but it does not constitute a limitation of the present invention. It should be understood that these examples are only used to illustrate the present invention and are not used to limit the scope of the present invention. In addition, the photochemical technology methods not described in detail in the examples are all conventional methods in the art, and the specific operations can be referred to the photochemical technology guide or product instructions.

[0049] Embodiment 1:

[0050] Water splitting reaction can effectively convert solar energy into hydrogen energy, which is one of the most promising ways to solve energy and environmental problems. However, due to the poor separation of photogenerated carriers, the efficiency of photocatalytic reaction is still not ideal. By preparing photocatalysts with different crystal faces, the built-in electric field between crystal faces is considered to be one of the effective ways to drive carrier separation. In recent years, applying external electric field in photocatalytic system is a more flexible and controllable strategy to improve carrier separation, which has attracted more and more attention from researchers. However, there are multiple processes in the electric field-assisted photocatalytic process: 1. Generation of photogenerated carriers; 2. Separation and transfer of photogenerated carriers driven by external field; 3. Redox reaction involving photogenerated carriers on the catalyst surface. At the same time, the efficiency of photocatalytic redox reaction on the catalyst surface is also affected by substrate adsorption, substrate conversion, and product desorption. These processes are not only spatiotemporal dynamics, but also involve surface reactions at the microscopic scale. In-depth understanding of the effect of electric field on specific photocatalytic processes requires real-time and in situ characterization technology to explore.

[0051] Single-molecule fluorescence microscopy (SMFM) has high temporal and spatial resolution and can be used to reveal the microscopic mechanism of photocatalysis. The present invention uses a self-built single-molecule super-resolution fluorescence imaging system to monitor the hole-induced oxidation reaction of Amplex red and the electron-induced reduction reaction of Resazurin on the surface of a single BiVO4 crystal, and explores the regulation mechanism of electric field on photocatalysis. Through single-molecule imaging of redox reactions, the present invention visualizes the spatial separation of photocatalytic redox reactions caused by the built-in electric field on different BiVO4 crystal planes. The direction and intensity of the external electric field can easily achieve the photogenerated electrons (e - ) and photogenerated holes (h + ) separation and accumulation, thereby effectively controlling the activity of the photocatalytic redox reaction, resulting in the dynamic redistribution behavior of the photocatalytic redox reaction. In addition, the single-molecule dynamics of the redox reaction under an external electric field were further analyzed. The results show that the adsorption process is the rate-limiting step of the photocatalytic redox reaction and is not affected by the external electric field. The dynamic redistribution behavior of the photocatalytic redox reaction is caused by the external electric field regulating the catalytic conversion process and the desorption process. The regulatory mechanism of the external electric field on the photocatalytic redox reaction was further demonstrated by the photocatalytic water splitting hydrogen analysis reaction (Hydrogen Evolution Reaction, HER) assisted by an external electric field.

[0052] Experimental steps for construction:

[0053] --Construction of electric field control device: In order to realize the single-molecule level monitoring of the photocatalytic redox reaction on the BiVO4 surface, the present invention firstly constructs an electric field control device. It consists of a working electrode (WE, ITO glass electrode 1, 0.15 mm thickness, 10 Ω cm -2 ), Counter Electrode, CE, ITO glass electrode 1 electrode, 1.0 mm thickness, 10 Ω cm -2 ) and a microchannel (double-sided tape 2, 2.0 cm × 5.0 mm × 150 μm). The liquid channel is composed of a double-sided tape 2 sandwiched between two ITO glass electrodes 1. The present invention deposits the BiVO4 sample on the microchannel substrate in a monodisperse state and fixes it at 90°C overnight.

[0054] ----Single-molecule fluorescence imaging of photocatalytic redox reactions on the surface of BiVO4 particles:

[0055] A solution containing 500 nM of a specific fluorescent probe (AR: Amplex Red) and 5 mM of a photogenerated carrier scavenger (AR solution containing photogenerated electron scavenger: MV 2+ The reaction solution of photogenerated hole scavenger: CH3OH in Re solution was continuously pumped into the liquid channel of the photoelectrochemical reaction cell (flow rate was 67 μL min -1 ). Under ultraviolet light (LED, 365nm), a photocatalytic redox reaction occurs on the BiVO4 surface to generate a catalytic product (Rf). A 561nm laser excites Rf to emit a fluorescence signal (the maximum emission wavelength is 590nm). When a bias voltage (V) is applied to WE, an electric field will be generated between WE and CE. By regulating the magnitude and direction of the bias voltage on the working electrode, the construction of an electric field with controllable magnitude and direction between the two electrodes is achieved. The fluorescence signal generated by Rf is collected using a 100× oil objective lens (HCX PL APO 100×0.47oil) and imaged using sCMOS (imaging rate is 30ms frame -1 );

[0056] All collected experimental data and single fluorescence images were processed using MetaMorph analysis software (Molecular Devices). The original single-molecule fluorescence time trace was processed by a 10% low-pass filter, and the single-molecule signal was screened by setting a threshold.

[0057] Finally, it should be noted that the above is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to perform equivalent replacements on parts thereof. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention. Although the above describes the specific implementation modes of the present invention in conjunction with the accompanying drawings, it is not intended to limit the protection scope of the present invention. Those skilled in the art should understand that, based on the technical solution of the present invention, various modifications or deformations that can be made by those skilled in the art without creative labor are still within the protection scope of the present invention.

Claims

1. An electric field in-situ control device suitable for single-molecule super-resolution fluorescence microscopy, characterized in that: include: Working electrode, counter electrode, microchannel, the working electrode and counter electrode are parallel electrode plates, the two electrode plates are bonded by double-sided adhesive with microchannels etched thereon to form a sandwich structure; the electrode plates are also provided with through holes, and are connected to the flow guide tube.

2. The electric field in-situ control device suitable for single-molecule super-resolution fluorescence microscopy according to claim 1, characterized in that: The working electrode and the counter electrode are both ITO glass electrodes; Or, the specific dimensions of the ITO glass electrode are: 2-3 cm in width, 4-5 cm in length, and 3-4 mm or 0.15-0.17 mm in thickness; Alternatively, the diameter of the through hole is 1-1.2 mm.

3. The electric field in-situ control device suitable for single-molecule super-resolution fluorescence microscopy according to claim 1, characterized in that: The size of the double-sided tape is 2.0-2.1 cm×5.0-5.1 mm×150-160 μm.

4. Application of the electric field in situ control device suitable for single-molecule super-resolution fluorescence microscopy as described in any one of claims 1 to 3 in single-molecule level quantitative detection during real-time in situ photocatalysis.

5. A method for constructing an electric field in situ control device suitable for single-molecule super-resolution fluorescence microscopy according to any one of claims 1 to 3, characterized in that: include: Clean the ITO glass electrode to meet the requirements of single-molecule imaging; The two cleaned ITO glass electrodes were punched and incubated with catalyst particles, respectively serving as the upper electrode and the lower electrode of the electric field control device; Two ITO glass electrodes are assembled into a sandwich structure by bonding them with double-sided tape etched with channels, and a flow guide tube is connected to the ITO glass electrode with holes so that the channels in the sandwich structure can be pumped with the reaction solution.

6. The method for constructing an electric field in situ control device suitable for single-molecule super-resolution fluorescence microscopy according to claim 5, characterized in that: The specific method of the cleaning treatment is: using glass cleaning agent and alcohol to repeatedly ultrasonically clean for 20-30 minutes, finally rinsing with water, drying with nitrogen, placing on a clean bench, and sealing for storage; Alternatively, in the catalyst incubation treatment of the ITO glass electrode, the ITO glass electrode used has a thickness of 0.15-0.17 mm, and the concentration of the catalyst particle aqueous solution is 3-4 mg / mL.

7. The method for constructing an electric field in situ control device suitable for single-molecule super-resolution fluorescence microscopy according to claim 5, characterized in that: The reaction solution includes: an oxidation reaction solution and a reduction reaction solution, respectively, a photogenerated electron scavenger MV in the AR solution 2+ And CH3OH, the photogenerated hole scavenger in Re solution.

8. Application of electric field control device suitable for single-molecule super-resolution fluorescence microscopy in regulating the photocatalytic redox reaction occurring on the surface of BiVO4 photocatalyst particles.

9. The use according to claim 8, characterized in that By performing detection and analysis under different electric fields through single-molecule level imaging, the super-resolution spatial distribution of the photocatalytic redox reaction occurring on the surface of BiVO4 photocatalyst particles was obtained.

10. The use according to claim 8, characterized in that The electric field control device is used to analyze the activity of the photocatalytic redox reaction occurring on the surface of the BiVO4 photocatalyst particles, and the frequency of the photocatalytic redox reaction occurring on the surface of the BiVO4 photocatalyst particles under different electric fields is quantified; and the control pathway of the photocatalytic redox reaction occurring on the surface of the BiVO4 photocatalyst particles under electric field control is analyzed through single molecule dynamics analysis.

Images