Microfluidic Raman spectrometer driving system based on STM32 integrated control

Abstract

The invention belongs to the technical field of integrated control of micro-fluidic analytical instruments and systems, and particularly discloses a micro-fluidic Raman spectrometer driving system based on STM32 integrated control, hardware comprises a handheld Raman spectrometer, a micro-fluidic chip, an intelligent serial port screen, a PCB (printed circuit board) and a peristaltic pump; the software comprises a password login module, a parameter setting module, an acquisition module, a transmission module, a storage module and a processing module; the Raman spectrometer, the serial port screen and the peristaltic pump are respectively connected with a serial port on the PCB, and then STM32CubeMX initialization configuration and C language programming in Keil are adopted, so that parameter control of the serial port screen on the spectrometer and the peristaltic pump is realized, and a series of key functions such as spectral data acquisition and processing and control of the flow rate of liquid in the micro-fluidic chip are realized. The integrated control platform has the characteristics of high integration, strong programmability and fast response speed, not only improves the automation level of the whole experimental system, but also realizes Raman spectrum detection and analysis of a to-be-detected substance on the micro-fluidic chip.

Description

Technical Field

[0001] The invention belongs to the technical field of microfluidic analytical instruments and system integrated control, and in particular relates to a microfluidic Raman spectrometer driving system based on STM32 integrated control. Background Art

[0002] Compared with traditional detection technologies, Raman spectroscopy has shown many significant advantages. It can realize real-time detection and obtain analysis results in the first time, which greatly improves work efficiency. Its detection speed is fast and can quickly complete the analysis of samples, which provides convenience for emergency situations or large-scale sample detection. At the same time, Raman spectroscopy has the characteristics of non-destructive detection and will not cause any damage to the samples, ensuring the integrity of the samples. The non-contact detection method avoids the risk of sample contamination. In addition, the amount of sample required for Raman spectroscopy is extremely small, and only a small amount of sample is needed for accurate analysis, saving sample resources. Moreover, the samples do not need to undergo complex pretreatment, which simplifies the detection process and reduces the detection cost.

[0003] However, traditional Raman spectrometers are usually large in size, which not only takes up more space, but also is not easy to move and carry, which limits its application scenarios to a certain extent. At the same time, the high price makes it only suitable for specific fields such as scientific research, and it is difficult to promote it in a wider range of practical applications. The handheld Raman spectrometer, with its small size, can be detected anytime and anywhere. The portability makes it very suitable for rapid detection of various substances on site, whether in the field environment outside the laboratory, industrial production site or emergency rescue occasions, it can play an important role. There are some shortcomings in the current handheld Raman spectrometers. These devices do not have integrated microfluidic chips and cannot achieve efficient processing and analysis of samples. At the same time, there is also a lack of automated detection and analysis functions, which requires operators to have high professional skills and experience, increasing the difficulty of detection and the risk of errors.

[0004] Therefore, it is of great practical significance to develop a handheld Raman spectrometer with superior performance, integrated microfluidic chip and automated detection and analysis. Summary of the invention

[0005] The purpose of the present invention is to overcome the defects of the prior art and provide a microfluidic Raman spectrometer driving system based on STM32 integrated control to solve the problem that the existing spectrometer has no integrated microfluidic chip and no automated detection and analysis.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A microfluidic Raman spectrometer driving system based on STM32 integrated control comprises hardware and software, wherein the hardware comprises a handheld Raman spectrometer, a microfluidic chip, an intelligent serial port screen, a PCB board, and a peristaltic pump, and the Raman spectrometer, the serial port screen, and the peristaltic pump are respectively connected to the serial port on the PCB board; and the software comprises a password login module, a parameter setting module, an acquisition module, a transmission module, a storage module, and a processing module.

[0008] In a preferred embodiment of the present invention, the password login module enters the main setting interface by inputting the correct password on the serial port screen, which also includes the functions of resetting the password and prompting the correct or incorrect password input.

[0009] In a preferred embodiment of the present invention, the parameter setting module sets the laser power, average times, integration time and rotation speed of the peristaltic pump of the Raman spectrometer through the serial port screen.

[0010] In a preferred embodiment of the present invention, the acquisition module drives the laser of the Raman spectrometer through the serial port screen to generate laser to acquire the Raman spectrum of the substance.

[0011] Further preferably, the spectrum is directly displayed on the screen after being collected, and the spectrum can also be magnified to observe the details.

[0012] In a preferred embodiment of the present invention, the storage module stores the collected spectral data so that it can be further analyzed and utilized by the user.

[0013] In a preferred embodiment of the present invention, the transmission module converts the format of the collected spectral data, and then uploads it to a computer via Bluetooth, making it convenient for users to analyze and organize the spectral data.

[0014] In a preferred embodiment of the present invention, the processing module performs judgment and analysis on the stored Raman spectrum data to determine whether the detected substance is negative or positive.

[0015] An application of a microfluidic Raman spectrometer driving system based on STM32 integrated control in Raman spectrum detection and analysis.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] 1. The present invention integrates a handheld Raman spectrometer, a microfluidic chip, a peristaltic pump, and an intelligent serial port screen, and controls the parameters of the spectrometer and the peristaltic pump through the serial port screen, thereby realizing a series of key functions such as spectral data acquisition and processing and controlling the liquid flow rate inside the microfluidic chip.

[0018] 2. The present invention has the characteristics of high integration, strong programmability and fast response speed. The integrated control platform not only improves the automation level of the entire experimental system, but also realizes the Raman spectrum detection and analysis of the substance to be tested on the microfluidic chip. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic diagram of the integrated control structure of the present invention;

[0020] Figure 2 It is the hardware instrument integration diagram of the present invention;

[0021] Figure 3 It is a detection flow chart of the present invention;

[0022] Figure 4 This is a trend diagram of the signal of a certain concentration of nano-signal gold detected in Example 1 of the present invention changing with the integration time;

[0023] Figure 5 This is a gold standard curve diagram for detecting nanometer signals in Example 1 of the present invention;

[0024] Figure 6 This is a diagram showing the results of the analysis of the myocardial infarction samples in Example 2 of the present invention. DETAILED DESCRIPTION

[0025] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0026] A microfluidic Raman spectrometer driving system based on STM32 integrated control comprises hardware and software, wherein the hardware comprises a handheld Raman spectrometer, a microfluidic chip, an intelligent serial port screen, a PCB board, and a peristaltic pump, and the Raman spectrometer, the serial port screen, and the peristaltic pump are respectively connected to the serial port on the PCB board; and the software comprises a password login module, a parameter setting module, an acquisition module, a transmission module, a storage module, and a processing module.

[0027] The password login module enters the main setting interface by inputting the correct password on the serial port screen, which also includes the functions of resetting the password and prompting the correct or incorrect password input.

[0028] The parameter setting module sets the laser power, average times, integration time and rotation speed of the peristaltic pump of the Raman spectrometer through the serial port screen.

[0029] The acquisition module drives the laser of the Raman spectrometer through the serial port screen to generate laser to collect the Raman spectrum of the material.

[0030] The spectrum is directly displayed on the screen after being collected, and the spectrum can also be magnified to observe the details.

[0031] The storage module stores the collected spectral data so that it can be further analyzed and utilized by users.

[0032] The transmission module converts the format of the collected spectral data, and then uploads it to a computer via Bluetooth, making it convenient for users to analyze and organize the spectral data.

[0033] The processing module judges and analyzes the stored Raman spectrum data, and then obtains the negative or positive of the detected substance.

[0034] An application of a microfluidic Raman spectrometer driving system based on STM32 integrated control in Raman spectrum detection and analysis.

[0035] Example 1

[0036] This embodiment discloses a microfluidic Raman spectrometer driving system based on STM32 integrated control. Figure 1 , Attachment Figure 2 , Attachment Figure 3 , is a microfluidic Raman spectrometer driving system based on STM32 integrated control of the present invention, specifically including: hardware and software, wherein the hardware includes a handheld Raman spectrometer, a microfluidic chip, an intelligent serial port screen, a PCB board, and a peristaltic pump, and the Raman spectrometer, the serial port screen, and the peristaltic pump are respectively connected to the serial port on the PCB board; the software includes a password login module, a parameter setting module, an acquisition module, a transmission module, a storage module, and a processing module. It integrates a handheld Raman spectrometer, a microfluidic chip, a peristaltic pump, and an intelligent serial port screen, and controls the parameters of the spectrometer and the peristaltic pump through the serial port screen, thereby realizing a series of key functions such as spectral data acquisition and processing and controlling the liquid flow rate inside the microfluidic chip. With its high degree of integration, strong programmability and fast response speed, the integrated control platform not only improves the automation level of the entire experimental system, but also realizes the Raman spectrum detection and analysis of the substance to be tested on the microfluidic chip.

[0037] In order to further optimize the above technical solution, the password login module enters the main setting interface by entering the correct password on the serial port screen, which also includes the functions of resetting the password and prompting the correct or incorrect password input.

[0038] In order to further optimize the above technical solution, the parameter setting module sets the laser power, average times, integration time and rotation speed of the peristaltic pump of the Raman spectrometer through the serial port screen.

[0039] In order to further optimize the above technical solution, the acquisition module drives the laser of the Raman spectrometer through the serial port screen to generate laser to collect the Raman spectrum of the material. After the spectrum is collected, it is directly displayed on the screen, and the spectrum can also be magnified to observe the details.

[0040] In order to further optimize the above technical solution, the storage module stores the collected spectral data so that it can be further analyzed and utilized by users.

[0041] In order to further optimize the above technical solution, the transmission module converts the format of the collected spectral data, and then uploads it to the computer via Bluetooth, which is convenient for users to analyze and organize the spectral data. After the present invention detects the nano-signal gold at different integration times, the data is uploaded to the computer via Bluetooth, and the spectrum after data analysis is shown in the attached figure. Figure 4 , Attachment Figure 5 As shown in the attached Figure 4 and attached Figure 5 The results show that by detecting the nano-signal gold at different integration times, its Raman spectrum intensity changes basically linearly, which means that the instrument has a certain accuracy in controlling the integration time and detecting the Raman spectrum intensity of the substance.

[0042] In order to further optimize the above technical solution, the processing module judges and analyzes the stored Raman spectrum data, and then obtains whether the nature of the detected substance is negative or positive.

[0043] Example 2

[0044] An application of a microfluidic Raman spectrometer driving system based on STM32 integrated control in Raman spectrum detection and analysis.

[0045] In this embodiment, 40 myocardial infarction samples provided by a hospital were divided into concentrations, 1-14pg / mL was negative (Negative), 15-100pg / mL was weakly positive (Weakly Positive), 100-500pg / mL was moderately positive (Moderately Positive), and greater than 500pg / mL was strongly positive (Strongly Positive), and a certain threshold (Threshold) was set. Then, the myocardial infarction samples were detected and analyzed according to the steps of the above-mentioned optimized technical solution. The obtained spectral data was uploaded to the computer via the instrument Bluetooth, and the results after using the data for analysis are shown in the attached figure. Figure 6 As shown, the feasibility of the instrument in the detection of myocardial infarction is demonstrated.

[0046] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some or all of the technical features thereof may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A microfluidic Raman spectrometer driving system based on STM32 integrated control, characterized in that: The invention comprises hardware and software. The hardware comprises a handheld Raman spectrometer, a microfluidic chip, an intelligent serial port screen, a PCB board, and a peristaltic pump. The Raman spectrometer, the serial port screen, and the peristaltic pump are respectively connected to the serial port on the PCB board; the software comprises a password login module, a parameter setting module, an acquisition module, a transmission module, a storage module, and a processing module.

2. The microfluidic Raman spectrometer driving system based on STM32 integrated control as claimed in claim 1, characterized in that: The password login module enters the main setting interface by inputting the correct password on the serial port screen.

3. The microfluidic Raman spectrometer driving system based on STM32 integrated control as claimed in claim 2, characterized in that: The password login module also includes password resetting and password prompting functions.

4. The microfluidic Raman spectrometer driving system based on STM32 integrated control as claimed in claim 1, characterized in that: The parameter setting module sets the laser power, average times, integration time and rotation speed of the peristaltic pump of the Raman spectrometer through the serial port screen.

5. The microfluidic Raman spectrometer driving system based on STM32 integrated control as claimed in claim 1, characterized in that: The acquisition module drives the laser of the Raman spectrometer through the serial port screen to generate laser to collect the Raman spectrum of the material.

6. The microfluidic Raman spectrometer driving system based on STM32 integrated control as claimed in claim 5, characterized in that: The spectrum is directly displayed on the screen after being collected.

7. The microfluidic Raman spectrometer driving system based on STM32 integrated control as claimed in claim 1, characterized in that: The storage module stores the collected spectral data so that it can be further analyzed and utilized by users.

8. The microfluidic Raman spectrometer driving system based on STM32 integrated control as claimed in claim 1, characterized in that: The transmission module converts the format of the collected spectral data, and then uploads it to a computer via Bluetooth, making it convenient for users to analyze and organize the spectral data.

9. The microfluidic Raman spectrometer driving system based on STM32 integrated control as claimed in claim 1, characterized in that: The processing module judges and analyzes the stored Raman spectrum data, and then obtains the negative or positive of the detected substance.

10. Application of the microfluidic Raman spectrometer driving system based on STM32 integrated control according to any one of claims 1 to 9 in Raman spectrum detection and analysis.

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