Real-time monitoring isolator for studying influence of microorganisms on host behaviors based on sterile mice

Abstract

The invention belongs to the technical field of biomedical experimental equipment, and discloses a real-time monitoring isolator for studying influence of microorganisms on host behaviors based on sterile mice, which comprises a mobile operation table, a support, a soft transparent isolator and a hard transparent glass isolator are arranged on the mobile operation table, the soft transparent isolator is communicated with the hard transparent glass isolator, and the support is provided with a plurality of through holes; partition plates are arranged in the hard transparent glass isolator so as to divide the internal space into a plurality of experiment areas. An infrared camera and a sound recognition sensor are mounted on the bracket and are used for monitoring multi-dimensional behaviors such as movement tracks, social interaction and sound characteristics of sterile mice in an experimental area in real time; and the upper computer is used for processing and analyzing the data. According to the isolator, a sterile environment is maintained, the problems of microbial interference, poor reliability of experimental results, equipment limitation, imperfect monitoring means and the like in the prior art are solved, and a more efficient, accurate and reliable experimental tool is provided for research on the relationship between intestinal flora and animal behaviors.

Description

Technical Field

[0001] The invention relates to the technical field of microorganism and animal behavior experimental equipment, and more specifically to a real-time monitoring isolator for studying the influence of microorganisms on host behavior based on sterile mice. Background Art

[0002] In the field of biology and medicine, the complex relationship between intestinal flora and host behavior has always been a hot topic of research. In recent years, with the rapid development of microbiome, neuroscience and bioinformatics, scientists have gradually revealed the important role of intestinal flora in regulating animal behavior. In particular, intestinal flora not only affects the physiological health of the host, but also regulates the host's social behavior, emotional response and cognitive function through multiple pathways such as the microbial-gut-brain axis. They have the ability to synthesize a variety of neurotransmitters (such as γ-aminobutyric acid, norepinephrine, dopamine, etc.) in the intestinal environment, which constitutes a potential and critical information exchange bridge between microorganisms and hosts. These metabolites produced by neuroactive bacteria can use diversified mechanisms to regulate brain function and host behavior. The specific methods include interaction with intestinal epithelial cells to affect the functionality of the intestinal barrier, activation of enteroendocrine cells to trigger the release of intestinal hormones, and indirect effects on the immune system and microglia by regulating dendritic cells, thereby showing a profound impact on the host's physiological state. In addition, many studies have shown that there is a significant correlation between intestinal flora and social behavior of animals. For example, in rodents (such as mice), the intestinal flora regulates the activity of the hypothalamus-pituitary-adrenal axis by affecting stress response neurons in the brain, thereby affecting their social behavior; at the same time, germ-free mice show significant social disorders compared to mice with normal intestinal flora, such as reduced interaction with unfamiliar mice, while the behavior of germ-free mice is significantly improved through flora transplantation. In addition, the colonization of specific bacterial communities such as Enterococcus faecalis can significantly improve the social ability of mice and reduce their stress hormone levels, which further confirms the close connection between intestinal flora and behavior. In order to further explore the causal relationship between flora and behavior, scientists have conducted a large number of intervention experiments. The study found that eliminating part of the flora in the mouse intestine through antibiotic treatment will significantly affect its social behavior, manifested as a decrease in social activities and an increase in stress hormone levels. Further studies have shown that these behavioral changes are closely related to the regulation of the hypothalamic-pituitary-adrenal axis by intestinal flora. Inhibiting the activity of the hypothalamic-pituitary-adrenal axis through pharmacological means, or knocking out glucocorticoid receptors in specific brain areas using gene editing technology can restore the social behavior of mice lacking intestinal flora to a certain extent, thus confirming the causal role of intestinal flora in regulating animal behavior.

[0003] As a special animal model with a "clean" microbial background, germ-free mice provide an ideal tool for studying the function of intestinal flora and its interaction with host behavior. Germ-free mice are experimental animals that are kept in a special breeding technique to ensure that they are not exposed to any exogenous microorganisms during birth and growth. It completely eliminates the potential interference of microorganisms on experimental results, allowing scientists to more clearly observe and analyze the effects of specific microorganisms or flora on host physiology, metabolism and behavior. Currently, the germ-free mouse model has become a key tool for revealing the intricate relationship between the microbiome and the health, behavioral state and physiological phenotype of the host. Its research is not limited to the simple comparison between the sterile and the germ-free state, but also goes deeper into the intervention of exogenous regulatory factors (such as drug treatment, surgical operations, specific dietary interventions, etc.), as well as the transplantation of single bacteria, multiple bacteria and even complex flora, to systematically analyze how specific functional bacteria or flora affect the physiological state, mental behavior and disease progression of mice. At present, although germ-free mice have been widely used in the study of the relationship between intestinal flora and animal behavior, traditional behavioral detection methods can only measure the behavior of animals at a specific time point in a bacterial environment, and this process will directly introduce interference from exogenous microorganisms, and it is impossible to simultaneously meet the needs of sterile or specific strains / flora conditions, real-time monitoring, and behavioral evaluation. At the same time, it is repeatedly mentioned in some behavioral experimental defects that there is currently a lack of equipment that can conduct behavioral experiments under completely sterile or specific flora (or strain) environmental conditions. Looking at the existing patented equipment, CN202111434546-a sterile mouse preparation and breeding integrated isolation device can realize the preparation of sterile mice based on embryo transplantation. Although it does not have the function of monitoring the behavior of sterile mice, it cannot analyze the effects of flora or strain changes on the short-term and long-term behavior of the host through real-time data, but its good sealing and light transmittance are still worth learning from. On this basis, this patent aims to focus on solving the technical problems of how to flexibly, accurately, and in real time comprehensively monitor and study the process of microbial influence on host behavior activities under different microbial background conditions such as sterility, flora or strain colonization. Summary of the invention

[0004] In view of this, the present invention provides an isolator that can conduct behavioral experiments on sterile mice in a completely sterile environment, and realize real-time dynamic monitoring of the behavior of sterile mice, so as to overcome the problems existing in the prior art such as microbial interference, poor reliability of experimental results, equipment limitations and imperfect monitoring methods.

[0005] In order to achieve the above object, the present invention adopts the following technical solution:

[0006] A real-time monitoring isolator for studying the effects of microorganisms on host behavior based on germ-free mice, including:

[0007] A mobile operating table, wherein a bracket is placed on the top of the mobile operating table, and a filter fan is placed on the bottom of the mobile operating table;

[0008] A soft transparent isolator for feeding, the soft transparent isolator is placed on the mobile operating table, a connecting sleeve is hot-pressed on one side wall of the soft transparent isolator, two first glove sleeves arranged at intervals are hot-pressed on the side of the soft transparent isolator facing the operator, a first operating glove is arranged in the cuff of the first glove sleeve away from the soft transparent isolator, an air intake cylinder filter and an exhaust cylinder filter are connected to the soft transparent isolator, the air intake cylinder filter is connected to the filter fan through a hose, and a transfer window is provided on the side of the soft transparent isolator opposite to the first glove sleeve;

[0009] A hard transparent glass isolator for experiments, the hard transparent glass isolator is placed on the bottom plate of the bracket, a first hard glass cylindrical opening is hot-melt-connected on one side of the hard transparent glass isolator, the connecting sleeve sealing sleeve is arranged on the first hard glass cylindrical opening, a second hard glass cylindrical opening is hot-melt-connected on the corresponding side of the hard transparent glass isolator, a second glove sleeve is connected to the second hard glass cylindrical opening, a second operating glove is arranged in the cuff of the second glove sleeve away from the hard transparent glass isolator, the hard transparent glass isolator has two partitions inside, handles are arranged on one side of the two partitions, and the two sides of the partitions are respectively plugged into the slots on the two opposite inner walls of the hard transparent glass isolator to divide the internal space of the hard transparent glass isolator into multiple experimental areas; an infrared camera and a sound recognition sensor located above the hard transparent glass isolator are installed on the bracket to realize real-time monitoring of the multi-dimensional behavior of the activity trajectory, social interaction, and sound characteristics of the sterile mice in the experimental area;

[0010] A host computer is electrically connected to the infrared camera and the sound recognition sensor for processing and analyzing data.

[0011] It can be seen from the above technical solution that compared with the prior art, the present invention discloses a real-time monitoring isolator based on sterile mice to study the influence of microorganisms on host behavior. The device can realize two independent processes of sterile mouse feeding and sterile mouse behavioral experiments at the same time. That is, the daily feeding, breeding and other management processes of sterile mice in a sterile environment are carried out in a soft transparent isolator. When conducting behavioral experiments, the sterile mice are driven from the soft transparent isolator to the experimental area in the hard transparent glass isolator for behavioral experiments. The infrared camera and sound recognition sensor externally mounted on the bracket can monitor the multi-dimensional behaviors of the activity trajectory, social interaction and sound characteristics of the sterile mice in the experimental area in real time, and analyze and evaluate these monitoring data through the host computer, so as to realize accurate and comprehensive monitoring and evaluation of the behavior of sterile mice in a sterile environment, and avoid the problem of poor results of behavioral experimental research caused by the use of ordinary mice.

[0012] In addition, by pulling down the partition, the internal space of the hard transparent glass isolator can be divided into multiple experimental areas, which is convenient for conducting open field experiments, social behavior test experiments on sterile mice, etc. It is very convenient to use, and the partition is set inside the hard transparent glass isolator and will not interfere with the sterile environment, avoiding the partition being set outside the hard transparent glass isolator and interfering with the sterile environment.

[0013] Therefore, the present invention is a real-time monitoring isolator for studying the influence of microorganisms on host behavior based on sterile mice, which organically integrates a soft transparent isolator for feeding and a hard transparent glass isolator for experiments, so that the sterile mice are kept in a sterile isolator throughout the entire process of feeding and experiment, thereby maintaining a sterile environment, overcoming the problems of microbial interference, poor reliability of experimental results, equipment limitations and imperfect monitoring methods in the prior art, and providing a more efficient, accurate and reliable experimental tool for the study of the relationship between intestinal flora and animal behavior.

[0014] Furthermore, the experimental area is provided with a forced swimming bucket for conducting a forced swimming experiment on sterile mice.

[0015] The beneficial effects of the above technical solution are: forced swimming experiments on germ-free mice can be carried out.

[0016] Furthermore, a rope for suspending germ-free mice is fixed on the top wall of the experimental area to conduct a tail suspension experiment on germ-free mice to study the effectiveness of antidepressant drugs.

[0017] The beneficial effect of adopting the above technical solution is that the tail suspension experiment of germ-free mice can be carried out to evaluate the efficacy of antidepressant drugs.

[0018] Furthermore, the bracket includes: the base plate, the column, and the cantilever rod, the base plate is placed on the top of the mobile operating table, the lower end of the column is fixedly connected to the top of the base plate, one end of the cantilever rod is rotatably connected to the upper end of the column, and the infrared camera and the sound recognition sensor are both installed at the other end of the cantilever rod.

[0019] The beneficial effect of adopting the above technical solution is that the positions of the infrared camera and the sound recognition sensor can be adjusted according to the experimental areas in different locations, so as to enable normal monitoring of the sterile mice in the corresponding experimental areas.

[0020] Furthermore, one end of the cantilever rod is connected to the upper end of the column by means of screws.

[0021] The beneficial effect of adopting the above technical solution is: by loosening the screw, the cantilever rod can be rotated, so that the position of the infrared camera and the sound recognition sensor can be adjusted, and then the infrared camera covers all experimental areas in the hard isolator, capturing the behavior and activities of the mice in real time.

[0022] Furthermore, a universal joint is fixed to the other end of the cantilever rod, and the universal rod on the universal joint is fixedly connected to the housing of the infrared camera.

[0023] The beneficial effect of adopting the above technical solution is: the infrared camera can be rotated in multiple directions, and then its position can be adjusted in multiple directions, so that the infrared camera has a better shooting angle, can fully cover the experimental area, and comprehensively monitor the behavior of sterile mice.

[0024] Furthermore, the mobile operating table includes: a first mobile platform and a second mobile platform, the soft transparent isolator is placed on the top surface of the first mobile platform, the filter fan is placed on the bottom of the first mobile platform, and the second mobile platform is pullable and arranged inside the first mobile platform. When the second mobile platform is pulled out, the bracket is placed on the top surface of the second mobile platform.

[0025] The beneficial effect of adopting the above technical solution is: when the isolator needs to be packaged and transported, the soft transparent isolator is deflated to make it in an uninflated state, and then the bracket and the hard transparent glass isolator are placed on the deflated soft transparent isolator or directly on the first mobile platform, and then the second mobile platform is retracted into the first mobile platform, so that the volume of the mobile operating platform can be reduced, which is convenient for packaging, transfer and transportation.

[0026] Furthermore, the first mobile platform includes:

[0027] A first platform, wherein the soft transparent isolator is placed on the top surface of the first platform, a receiving cavity is provided on one side surface of the first platform, four first supporting legs are fixed to the bottom end of the first platform, each of the first supporting legs is provided with a first moving wheel, and a first longitudinal beam tube is fixed between two first supporting legs on the same side;

[0028] A first reinforced cross brace tube, wherein the first reinforced cross brace tubes are two and are respectively fixed between two of the first longitudinal beam tubes, and the filter fan is fixed on the two first longitudinal beam tubes;

[0029] The second mobile platform comprises:

[0030] A second platform, one side of the second platform is drawable and arranged in the accommodating cavity, the bracket is placed on the top surface of the second platform, two second supporting legs are fixed to the bottom end of the other side of the second platform, each of the second supporting legs is equipped with a second moving wheel, and a second longitudinal beam tube is fixed between the two second supporting legs;

[0031] The second reinforcing cross bracing tubes include two second reinforcing cross bracing tubes, one end of each of which is fixedly connected to the second longitudinal beam tube, and the other end of each of which is respectively inserted into the two first reinforcing cross bracing tubes.

[0032] The beneficial effect of adopting the above technical solution is: when the second mobile platform is folded into the first mobile platform, the second platform is pushed into the accommodating cavity, and at the same time the second reinforced cross brace tube is folded into the first reinforced cross brace tube, so that the second mobile platform can be folded into the first mobile platform; when it is needed, the second mobile platform can be directly pulled out of the accommodating cavity, and then the bracket and the hard transparent glass isolator are placed on the second mobile platform, and then the filter fan is used to inflate the soft transparent isolator through the air intake cylindrical filter to make it a transparent package.

[0033] Furthermore, two fastening bolts are threadedly connected at intervals on one side surface of the first platform, and a fastening bolt hole is opened on one side surface of the second platform. The two fastening bolts can be respectively plugged or threadedly connected to the fastening bolt holes.

[0034] The beneficial effects of adopting the above technical solution are: when the second mobile platform is folded into the first mobile platform, it is fixed by the first fastening bolt to prevent it from being pulled out due to external force during transportation; when the second mobile platform is pulled out of the accommodating cavity, it is fixed with the second fastening bolt to prevent it from being pushed into the accommodating cavity again due to accidental operation due to external force. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0036] Figure 1 This is a schematic diagram of the front structure of the real-time monitoring isolator for studying the effects of microorganisms on host behavior based on germ-free mice provided by the present invention.

[0037] Figure 2 for Figure 1 Schematic diagram of the enlarged structure of local A in the middle.

[0038] Figure 3 This is a schematic diagram of the rear structure of the real-time monitoring isolator for studying the effects of microorganisms on host behavior based on germ-free mice provided by the present invention.

[0039] Figure 4 Schematic diagram of the perspective structure of a hard transparent glass isolator. DETAILED DESCRIPTION

[0040] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0041] like Figure 1-Figure 4 The embodiment of the present invention discloses a real-time monitoring isolator for studying the influence of microorganisms on host behavior based on germ-free mice. The isolator can conduct behavioral experiments in a completely sterile environment and realize real-time dynamic monitoring of the behavior of germ-free mice. The isolator includes:

[0042] A mobile operating table 1, a bracket 2 is placed on the top of the mobile operating table 1, and a filter fan 3 is placed on the bottom of the mobile operating table 1;

[0043] A soft transparent isolator 4 for feeding (made of high-strength, corrosion-resistant PVC material to ensure the firmness and durability of the isolator), the soft transparent isolator 4 is placed on the mobile operating table 1, a connecting sleeve 5 is hot-pressed on one side wall of the soft transparent isolator 4, two first glove sleeves 6 arranged at intervals are hot-pressed on the side of the soft transparent isolator 4 facing the operator, a first operating glove 7 is arranged in the cuff of the first glove sleeve 6 away from the soft transparent isolator 4, an air intake cylinder filter 8 and an exhaust cylinder filter 9 are connected to the soft transparent isolator 4, the air intake cylinder filter 8 is connected to the filter fan 3 through a hose, and a transfer window 10 is provided on the side of the soft transparent isolator 4 opposite to the first glove sleeve 6;

[0044] A hard transparent glass isolator 11 for experiment (made of quartz glass to ensure high light transmittance in the experimental area and camera clarity) is placed on the bottom plate 21 of the bracket 2. A first hard glass cylindrical opening 12 is connected by hot-melt on one side of the hard transparent glass isolator 11. A connecting sleeve 5 sealing sleeve is arranged on the first hard glass cylindrical opening 12. A second hard glass cylindrical opening 13 is connected by hot-melt on the corresponding side of the hard transparent glass isolator 11. A second glove sleeve 14 is connected to the second hard glass cylindrical opening 13. The second glove sleeve 14 is away from the hard transparent glass isolator. A second operating glove 15 is provided in the cuff of 11, and two partitions 16 are provided inside the hard transparent glass isolator 11. A handle 161 is provided on one side of each of the two partitions 16. The two sides of the partition 16 are respectively plugged into the slots 111 on the two opposite inner walls of the hard transparent glass isolator 11, so as to divide the internal space of the hard transparent glass isolator 11 into a plurality of experimental areas 112; an infrared camera 17 and a sound recognition sensor 18 located above the hard transparent glass isolator 11 are installed on the bracket 2, which are used to realize real-time monitoring of the multi-dimensional behaviors of the activity trajectory, social interaction, and sound characteristics of the sterile mice in the experimental area 112;

[0045] The host computer 19 is electrically connected to the infrared camera 17 and the sound recognition sensor 18 for processing and analyzing the data.

[0046] A forced swimming bucket 20 for conducting a forced swimming experiment on germ-free mice is provided in the experimental area 112 .

[0047] A hanging rope 26 for hanging sterile mice is fixed on the top wall of the experimental area 112 so as to perform a tail suspension experiment on sterile mice.

[0048] Of course, the forced swimming bucket 20 and the hanging rope 26 can be pre-integrated in the experimental area, or a sterile transfer cabin (not shown) can be set on the hard transparent glass isolator 11 to facilitate researchers to operate and transfer experimental materials in a sterile environment, such as taking and placing the forced swimming bucket 20 (which needs to be completely sterilized) and installing the hanging rope 26 (which needs to be completely sterilized).

[0049] The bracket 2 includes: a base plate 21, a column 22, and a cantilever rod 23. The base plate 21 is placed on the top of the mobile operating platform 1. The lower end of the column 22 is fixedly connected to the top of the base plate 21. One end of the cantilever rod 23 is rotatably connected to the upper end of the column 22. The infrared camera 17 and the sound recognition sensor 18 are both installed at the other end of the cantilever rod 23.

[0050] One end of the cantilever rod 23 is connected to the upper end of the column 22 via a screw 24 .

[0051] A universal joint 25 is fixed to the other end of the cantilever rod 23 , and a universal rod 251 on the universal joint 25 is fixedly connected to the housing of the infrared camera 17 .

[0052] The mobile operating table 1 includes: a first mobile platform 101 and a second mobile platform 102. A soft transparent isolator 4 is placed on the top surface of the first mobile platform 101, a filter fan 3 is placed on the bottom of the first mobile platform 101, and the second mobile platform 102 is retractably arranged inside the first mobile platform 101. When the second mobile platform 102 is pulled out, the bracket 2 is placed on the top surface of the second mobile platform 102.

[0053] The first mobile platform 101 includes:

[0054] A first platform 1011, a soft transparent isolator 4 is placed on the top surface of the first platform 1011, a receiving cavity 10111 is opened on one side surface of the first platform 1011, four first supporting legs 1012 are fixed to the bottom end of the first platform 1011, each first supporting leg 1012 is installed with a first moving wheel 1013, and a first longitudinal beam tube 1014 is fixed between two first supporting legs 1012 on the same side;

[0055] First reinforcing cross bracing tubes 1015, there are two first reinforcing cross bracing tubes 1015, which are respectively fixed between two first longitudinal beam tubes 1014, and the filter fan 3 is fixed on the two first longitudinal beam tubes 1014;

[0056] The second mobile platform 102 includes:

[0057] A second platform 1021, one side of the second platform 1021 is drawable and arranged in the accommodating cavity 10111, the bracket 2 is placed on the top surface of the second platform 1021, two second supporting legs 1022 are fixed to the bottom end of the other side of the second platform 1021, each second supporting leg 1022 is installed with a second moving wheel 1023, and a second longitudinal beam tube 1024 is fixed between the two second supporting legs 1022;

[0058] The second reinforcing cross bracing tube 1025 , there are two second reinforcing cross bracing tubes 1025 , one end of each of which is fixedly connected to the second longitudinal beam tube 1024 , and the other end of each of which is respectively inserted into the two first reinforcing cross bracing tubes 1015 .

[0059] Two fastening bolts 27 are threadedly connected at intervals on one side of the first platform 1011 , and a fastening bolt hole is provided on one side of the second platform 1021 . The two fastening bolts 27 can be respectively plugged or threadedly connected to the fastening bolt holes.

[0060] The isolator of the present invention has the following advantages:

[0061] (1) Completely sterile environment: Provide a completely sterile experimental environment to avoid interference from external microorganisms and ensure the accuracy and repeatability of experimental results. In addition, the temperature and humidity of the gas entering the soft transparent isolator can be adjusted by the filter fan, so that the living environment of the sterile mice is in the best state. In addition, the isolator can be placed in a mouse room, which has an adjustable light source that can adjust the light intensity, further providing a comfortable lighting environment for the sterile mice. In addition, the soft transparent isolator can isolate external noise and provide a comfortable living environment for the sterile mice.

[0062] (2) Real-time dynamic monitoring: Equipped with an advanced monitoring system, it can comprehensively and detailedly record the behavioral data of mice, providing researchers with reliable experimental data support.

[0063] (3) Strong adaptability: The design is flexible and compatible with a variety of behavioral experimental equipment to meet different experimental needs, such as:

[0064] Forced swim barrel: Commonly used to study the effectiveness of antidepressant drugs and to assess the behavioral responses of animals under stressful situations.

[0065] Open field (can be conducted in the experimental area): used to assess the anxiety level and activity of animals, and to analyze their behavioral performance by observing their movement trajectory and residence time in the open field.

[0066] Tail suspension: Mainly used to evaluate the efficacy of antidepressant drugs and to assess the coping strategies and behavioral responses of animals under inescapable stressful conditions.

[0067] Social behavior test: By setting up multiple experimental areas, the interaction between mice and their companions is recorded to evaluate the social behavior and social avoidance behavior of mice.

[0068] Among them, the mouse tail suspension test and the open field test are two commonly used behavioral tests used to evaluate the depressive-like behavior, anxiety level, and motor activity of mice. Mouse tail suspension test: The mouse's tail is suspended to observe its struggle and immobility time. Longer immobility time is usually regarded as a sign of depressive-like behavior. Open field test: The mouse is placed in an open open field, and its activity time in the central area and the edge area is recorded. The increase in the exploration time in the central area is regarded as a sign of reduced anxiety level.

[0069] The impact of intestinal flora on these behavioral experiments has attracted increasing attention in recent years. The gut-brain axis refers to a two-way communication channel between intestinal microorganisms and the central nervous system. The intestinal flora affects brain function and behavior by producing neurotransmitters, short-chain fatty acids, and immune regulation. In mouse models, changes in the intestinal flora can significantly affect their behavior. For example, studies have found that the feces of aging individuals were transplanted into germ-free mice through the microbiota transplantation technique, which reproduced the phenotypic changes that occur with aging, including impaired cognitive ability, changes in intestinal flora, and reduced production of short-chain fatty acids. In the germ-free mice of the transplant recipients, the struggling time in the tail suspension experiment was significantly lower than that of normal mice, the exploration time of the central domain in the open field experiment was significantly shorter than that of normal mice, and the immobility time was significantly higher than that of normal mice. These behavioral changes can be reversed by supplementing probiotics or restoring normal flora.

[0070] Studies have shown that the intestinal flora can affect the results of the tail suspension test by regulating the host's brain function and behavior. Normal intestinal flora helps maintain emotional stability, while disorders of the intestinal flora (such as those caused by antibiotics, sterile environments, or unhealthy diets) may increase depressive-like behaviors, which are manifested as increased immobility time in mice in the tail suspension test. When intestinal flora from patients with depression are transplanted into mice, the mice's depressive-like behaviors in the tail suspension test are often aggravated; while transplanting the flora of healthy individuals into depression model mice helps reduce depressive-like behaviors. The effects of some antidepressant drugs may be partially achieved by regulating the intestinal flora. For example, certain antidepressants, such as selective serotonin reuptake inhibitors, have been found to affect the composition and metabolic function of the intestinal flora. This means that the intestinal flora may be involved in drug-induced antidepressant effects.

[0071] Studies have shown that after supplementing mice with lactobacillus, the mice's immobility time in the tail suspension test and forced swimming test was significantly reduced, showing an anti-depressant effect. Through this reaction, further research found that this lactobacillus changed the activity of brain areas related to anxiety and depression by affecting the expression of γ-aminobutyric acid (GABA) receptors.

[0072] (4) Easy operation: Humanized design, easy operation and maintenance, improving experimental efficiency.

[0073] (5) The mobile operating table is retractable to reduce the volume and facilitate the overall packaging and transportation of the isolator.

[0074] In this specification, each embodiment is described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the embodiments can be referred to each other. For the device disclosed in the embodiment, since it corresponds to the method disclosed in the embodiment, the description is relatively simple, and the relevant parts can be referred to the method part.

[0075] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A real-time monitoring isolator based on germ-free mice to study the effects of microorganisms on host behavior, characterized by: include: A mobile operating platform (1), wherein a bracket (2) is placed on the top of the mobile operating platform (1), and a filter fan (3) is placed on the bottom of the mobile operating platform (1); A soft transparent isolator (4) for feeding, the soft transparent isolator (4) being placed on the mobile operating table (1), a connecting sleeve (5) being hot-pressed on one side wall of the soft transparent isolator (4), two first glove sleeves (6) being hot-pressed on the side of the soft transparent isolator (4) facing the operator, a first operating glove (7) being arranged in the cuff of the first glove sleeve (6) away from the soft transparent isolator (4), an air intake cylindrical filter (8) and an air exhaust cylindrical filter (9) being connected to the soft transparent isolator (4), the air intake cylindrical filter (8) being connected to the filter fan (3) via a hose, and a transfer window (10) being provided on the side of the soft transparent isolator (4) opposite to the first glove sleeve (6); A hard transparent glass isolator (11) for experiment, the hard transparent glass isolator (11) is placed on the bottom plate (21) of the bracket (2), a first hard glass cylindrical opening (12) is connected by hot-melt on one side of the hard transparent glass isolator (11), the connecting sleeve (5) is sealed on the first hard glass cylindrical opening (12), a second hard glass cylindrical opening (13) is connected by hot-melt on the corresponding side of the hard transparent glass isolator (11), a second glove sleeve (14) is connected to the second hard glass cylindrical opening (13), and a second operating glove is provided in the cuff of the second glove sleeve (14) away from the hard transparent glass isolator (11). (15), the hard transparent glass isolator (11) has two partitions (16) inside, and a handle (161) is provided on one side of the two partitions (16), and the two sides of the partitions (16) are respectively plugged into the slots (111) on the two opposite inner walls of the hard transparent glass isolator (11) to divide the internal space of the hard transparent glass isolator (11) into a plurality of experimental areas (112); the bracket (2) is equipped with an infrared camera (17) and a sound recognition sensor (18) located above the hard transparent glass isolator (11), which are used to realize real-time monitoring of the multi-dimensional behavior of the activity trajectory, social interaction, and sound characteristics of the sterile mice in the experimental area (112); A host computer (19), the host computer (19) is electrically connected to the infrared camera (17) and the sound recognition sensor (18) and is used for processing and analyzing data.

2. The real-time monitoring isolator for studying the effects of microorganisms on host behavior based on germ-free mice according to claim 1 is characterized in that: The experimental area (112) is provided with a forced swimming bucket (20) for conducting a forced swimming experiment on sterile mice.

3. The real-time monitoring isolator for studying the effects of microorganisms on host behavior based on germ-free mice according to claim 1 is characterized in that: A hanging rope (26) for hanging sterile mice is fixed on the top wall of the experimental area (112) so as to carry out a tail hanging experiment on sterile mice.

4. The real-time monitoring isolator for studying the effects of microorganisms on host behavior based on germ-free mice according to claim 1, characterized in that: The bracket (2) comprises: a base plate (21), a column (22), and a cantilever rod (23); the base plate (21) is placed on the top of the mobile operating platform (1); the lower end of the column (22) is fixedly connected to the top of the base plate (21); one end of the cantilever rod (23) is rotatably connected to the upper end of the column (22); and the infrared camera (17) and the sound recognition sensor (18) are both installed at the other end of the cantilever rod (23).

5. The real-time monitoring isolator for studying the effects of microorganisms on host behavior based on germ-free mice according to claim 4 is characterized in that: One end of the cantilever rod (23) is connected to the upper end of the column (22) via a screw (24).

6. The real-time monitoring isolator for studying the effects of microorganisms on host behavior based on germ-free mice according to claim 4, characterized in that: A universal joint (25) is fixed to the other end of the cantilever rod (23), and the universal rod (251) on the universal joint (25) is fixedly connected to the housing of the infrared camera (17).

7. The real-time monitoring isolator for studying the effects of microorganisms on host behavior based on germ-free mice according to any one of claims 1 to 6, characterized in that: The mobile operating table (1) comprises: a first mobile platform (101) and a second mobile platform (102); the soft transparent isolator (4) is placed on the top surface of the first mobile platform (101); the filter fan (3) is placed on the bottom of the first mobile platform (101); the second mobile platform (102) is drawable and arranged inside the first mobile platform (101); when the second mobile platform (102) is drawn out, the bracket (2) is placed on the top surface of the second mobile platform (102).

8. The real-time monitoring isolator for studying the effects of microorganisms on host behavior based on germ-free mice according to claim 7, characterized in that: The first mobile platform (101) comprises: A first platform (1011), the soft transparent isolator (4) is placed on the top surface of the first platform (1011), a receiving cavity (10111) is provided on one side surface of the first platform (1011), Four first supporting legs (1012) are fixed at the bottom end of the first platform (1011), each of the first supporting legs (1012) is mounted with a first moving wheel (1013), and a first longitudinal beam tube (1014) is fixed between two first supporting legs (1012) located on the same side; A first reinforcing cross bracing tube (1015), wherein the first reinforcing cross bracing tubes (1015) are two and are respectively fixed between two of the first longitudinal beam tubes (1014), and the filter fan (3) is fixed on the two first longitudinal beam tubes (1014); The second mobile platform (102) comprises: A second platform (1021), one side of the second platform (1021) is drawable and arranged in the accommodating cavity (10111), the bracket (2) is placed on the top surface of the second platform (1021), two second supporting legs (1022) are fixed to the bottom end of the other side of the second platform (1021), each of the second supporting legs (1022) is mounted with a second moving wheel (1023), and a second longitudinal beam tube (1024) is fixed between the two second supporting legs (1022); The second reinforcing cross bracing tube (1025) is two in number, one end of each of which is fixedly connected to the second longitudinal beam tube (1024), and the other end of each of which is respectively inserted into the two first reinforcing cross bracing tubes (1015).

9. The real-time monitoring isolator for studying the effects of microorganisms on host behavior based on germ-free mice according to claim 8, characterized in that: Two fastening bolts (27) are threadedly connected at intervals on one side surface of the first platform (1011), and a fastening bolt hole is opened on one side surface of the second platform (1021). The two fastening bolts (27) can be respectively plugged or threadedly connected to the fastening bolt holes.

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