Mosquito repelling composition based on pandan leaf extract

Abstract

The invention discloses a mosquito repelling composition based on pandan leaf extract, and belongs to the technical field of mosquito repelling. The mosquito repelling composition based on the pandan leaf extract is prepared from the following raw materials in parts by mass: 15 to 27 parts of pandan leaf extract, 4 to 8 parts of geranium extract, 2 to 5 parts of lavender extract, 1 to 3 parts of mint extract, 10 to 15 parts of grape seed oil and 40 to 70 parts of solvent. Through the combined action of various plant extracts with the mosquito-repelling effect, the excellent mosquito-repelling effect is synergistically achieved. The prepared pandan leaf extract is rich in effective components such as flavonoid compounds and volatile oil, the released smell can effectively repel mosquitoes, the normal physiological activity of the mosquitoes is influenced, and the pandan leaf extract has obvious repelling activity.

Description

Technical Field

[0001] The invention relates to the technical field of mosquito repellent, in particular to a mosquito repellent composition based on pandan leaf extract. Background Art

[0002] Mosquitoes are small insects belonging to the family Culicidae in the order Diptera, and are distributed worldwide. For many years, the problem of mosquitoes easily breeding under the influence of high temperature and high humidity has not been solved, which continues to affect the normal life of human beings. Large-scale mosquito activities will further increase the risk of spreading related infectious diseases such as malaria, epidemic encephalitis B, dengue fever, etc., and endanger human health.

[0003] With the continuous improvement of people's living standards, the problem of how to effectively prevent and repel mosquitoes has gradually received more attention. The existing mainstream mosquito repellent products include floral water, essential oils, wind oil essence, mosquito coils, etc. Although they have good mosquito repellent effects, they generally have problems such as strong irritation, poor durability, and some of the raw materials are toxic or even carcinogenic to the human body. Moreover, these mainstream mosquito repellent products mainly rely on the active ingredients such as camphor, pyrethrin, prallethrin, pyrethrin, citronellal, etc. to play a mosquito repellent effect, but these ingredients easily make mosquitoes resistant. Therefore, it is urgent for researchers in this field to design a new type of mosquito repellent product with low irritation, strong durability, and non-toxic and harmless characteristics.

[0004] Pandan leaves, also known as fragrant pandanus and colorful leaves, are perennial herbaceous tropical spice plants, famous for their unique aroma. Its fragrance is fresh and elegant, widely accepted by the public, and relatively cheap. In addition, recent studies have found that the active ingredients in pandan leaves have a certain effect on repelling insects. Therefore, how to design a mosquito repellent product using pandan as raw material has great research value. Summary of the invention

[0005] The object of the present invention is to provide a mosquito repellent composition based on pandan leaf extract to solve the above-mentioned problems in the background technology. The present invention achieves an excellent mosquito repellent effect through the joint action of multiple plant extracts with mosquito repellent effect. The pandan leaf extract prepared by the present invention is rich in effective ingredients such as flavonoids and volatile oils, and the odor released by it can effectively repel mosquitoes, affect the normal physiological activities of mosquitoes, and has obvious repellent activity.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] One of the technical solutions of the present invention is to provide a mosquito repellent composition based on pandan leaf extract, wherein the raw materials, calculated by weight, include:

[0008] 15-27 parts of pandan leaf extract, 4-8 parts of geranium extract, 2-5 parts of lavender extract, 1-3 parts of peppermint extract, 10-15 parts of grape seed oil and 40-70 parts of solvent.

[0009] Preferably, the preparation method of the pandan leaf extract is supercritical carbon dioxide extraction.

[0010] Preferably, the supercritical carbon dioxide extraction comprises the following steps:

[0011] The pandan leaves are crushed to pass through a 25-80 mesh sieve, and then placed in an extraction kettle of a supercritical carbon dioxide extractor for cyclic extraction. The raw material system is then passed into a separation kettle for collection to obtain a pandan leaf extract.

[0012] Preferably, the conditions of the cyclic extraction are: extraction pressure of 15-20 MPa, extraction temperature of 40-50° C., entrainer of 70-85 vol% ethanol aqueous solution, and carbon dioxide flow rate of 10-15 L / min.

[0013] Preferably, the raw materials are, by mass,:

[0014] 26 parts of pandan leaf extract, 7 parts of geranium extract, 3 parts of lavender extract, 2 parts of peppermint extract, 15 parts of grape seed oil and 60 parts of solvent.

[0015] Preferably, the solvent is ethanol.

[0016] The second technical solution of the present invention is to provide a method for preparing the above-mentioned mosquito repellent composition based on pandan leaf extract, comprising the following steps:

[0017] The raw materials are mixed to obtain the mosquito repellent composition based on the pandan leaf extract.

[0018] The third technical solution of the present invention is to provide an application of the above-mentioned mosquito repellent composition based on pandan leaf extract in the preparation of products with mosquito repellent effect.

[0019] The present invention increases the content of active substances in the raw materials by controlling the temperature, pressure and other conditions of the extraction process. By improving the extraction process, the mosquito repellent composition of the present invention can achieve a higher mosquito repellent effect.

[0020] The beneficial technical effects of the present invention are as follows:

[0021] The present invention achieves excellent mosquito repellent effect through the joint action of multiple plant extracts with mosquito repellent effect. The pandan leaf extract prepared by the present invention is rich in effective ingredients such as flavonoids and volatile oils, and the odor released by the extract can effectively repel mosquitoes, affect the normal physiological activities of mosquitoes, and has obvious repellent activity.

[0022] In addition, the mosquito repellent composition prepared by the present invention retains a large amount of linoleic acid, which is mainly derived from pandan leaves, and can improve the storage stability of the mosquito repellent composition, so that the mosquito repellent effect is retained for a longer time, and the mosquito repellent effect is achieved by working together with other ingredients in the composition.

[0023] The present invention has been found through experimental testing that the addition of geranium extract can greatly improve the odor acceptance of the product, make the fragrance more natural, and reduce the odor irritation.

[0024] The extraction process designed by the present invention is simple and easy to operate, and has low requirements on equipment. The present invention uses natural raw materials as a formula, does not contain chemical components such as pyrethrins and DEET, is safe and non-toxic, has a light smell and is non-irritating, and has good application value. In addition, the mosquito repellent composition prepared by the present invention also has the effects of soothing nerves and improving sleep. DETAILED DESCRIPTION

[0025] Now, various exemplary embodiments of the present invention are described in detail, and this detailed description should not be considered as a limitation of the present invention, but should be understood as a more detailed description of certain aspects, characteristics and embodiments of the present invention. It should be understood that the terms described in the present invention are only for describing specific embodiments and are not used to limit the present invention.

[0026] In addition, for the numerical range in the present invention, it is understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. The intermediate value in any stated value or stated range, and each smaller range between any other stated value or intermediate value in the range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0027] Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which the invention pertains. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein may also be used in the practice or testing of the present invention.

[0028] The words “include,” “including,” “have,” “contain,” etc. used in the present invention are open-ended terms, meaning including but not limited to.

[0029] All raw materials used in the following examples and comparative examples of the present invention are commercially available products.

[0030] Example 1

[0031] A mosquito repellent composition, comprising the following raw materials in parts by weight:

[0032] 26 parts of pandan leaf extract, 7 parts of geranium extract, 3 parts of lavender extract, 2 parts of peppermint extract, 15 parts of grape seed oil, and 60 parts of ethanol (95 vol%).

[0033] The preparation method of pandan leaf extract is as follows: wash the pandan leaves, put them into a 40°C oven to dry to constant weight, crush them until they pass through a 25-mesh sieve, and then put them into an extraction kettle of a supercritical carbon dioxide extractor for cyclic extraction; the conditions of the cyclic extraction are: the extraction pressure is 20MPa, the extraction temperature is 45°C, the entrainer is 200mL of 80vol% ethanol aqueous solution, and the carbon dioxide flow rate is 13L / min; after the extraction is completed, the raw material system is passed into a separation kettle at 25°C, collected, and the pandan leaf extract is obtained.

[0034] The preparation method of geranium extract is as follows: wash geranium flowers, put them into a 40°C oven to dry to constant weight, then grind them to pass through a 25-mesh sieve, put them into a sample bottle, mix them with a 60 vol% ethanol aqueous solution at a solid-liquid ratio of 1g:10mL, extract them for 40 minutes at a temperature of 25°C and an ultrasonic frequency of 30kHz, then concentrate them under reduced pressure to remove the solvent, and obtain the geranium extract.

[0035] The preparation method of lavender extract is as follows: wash the lavender, put it into a 40°C oven to dry to constant weight, then grind it to pass through a 25-mesh sieve, put it into a sample bottle, mix it with a 60 vol% ethanol aqueous solution at a solid-liquid ratio of 1g:10mL, extract it for 40 minutes at a temperature of 25°C and an ultrasonic frequency of 30kHz, then concentrate it under reduced pressure to remove the solvent, and obtain the lavender extract.

[0036] The preparation method of the mint extract is as follows: wash the mint leaves, put them into a 40°C oven to dry to constant weight, then crush them to pass through a 25-mesh sieve, put them into a sample bottle, mix them with a 60 vol% ethanol aqueous solution at a solid-liquid ratio of 1g:10mL, extract them for 40 minutes at a temperature of 25°C and an ultrasonic frequency of 30kHz, then concentrate them under reduced pressure to remove the solvent, and obtain the mint extract.

[0037] Preparation of mosquito repellent composition:

[0038] The pandan leaf extract, geranium extract, lavender extract, mint extract, grape seed oil and ethanol are mixed to obtain a mosquito repellent composition.

[0039] Example 2

[0040] The only difference from Example 1 is that it is composed of the following raw materials in parts by mass:

[0041] 17 parts of pandan leaf extract, 4 parts of geranium extract, 5 parts of lavender extract, 1 part of peppermint extract, 10 parts of grape seed oil, and 40 parts of ethanol (95 vol%).

[0042] Comparative Example 1

[0043] The only difference from Example 1 is that the type of entrainer in the process of preparing the pandan leaf extract is changed to an equal volume of 80 vol% methanol aqueous solution.

[0044] Comparative Example 2

[0045] The only difference from Example 1 is that the preparation method of the pandan leaf extract is modified as follows:

[0046] Wash the pandan leaves, put them into an oven at 40°C to dry to constant weight, then crush them to pass through a 25-mesh sieve, put them in a sample bottle, mix them with 60 vol% ethanol aqueous solution at a solid-liquid ratio of 1g:10mL, extract them at 25°C and an ultrasonic frequency of 30kHz for 40 minutes, then concentrate under reduced pressure to remove the solvent to obtain the pandan leaf extract.

[0047] Comparative Example 3

[0048] The difference from Example 1 is only that the pandan leaves are replaced with the mugwort leaves of equal quality.

[0049] Comparative Example 4

[0050] The only difference from Example 1 is that the addition of geranium extract, lavender extract and peppermint extract is omitted and an equal mass of pandan leaf extract is added.

[0051] Comparative Example 5

[0052] The only difference from Example 1 is that the addition of geranium extract is omitted and an equal mass of pandan leaf extract is added.

[0053] Comparative Example 6

[0054] The only difference from Example 1 is that the addition of grape seed oil is omitted and an equal mass of ethanol (95 vol%) is added.

[0055] Effect verification

[0056] (1) A mosquito repellent test was conducted in accordance with GB / T 13917.9-2009. A total of 16 test subjects were tested by applying the above mosquito repellent composition (application amount 1.5 μL / cm 2 ) method to test the repellent effect on Aedes albopictus and calculate the effective protection time. The test results are shown in Table 1.

[0057] Table 1

[0058]

[0059]

[0060] (2) The fragrance retention effect, fragrance acceptance, fragrance irritation and storage stability of the mosquito repellent composition were tested. The test results are shown in Table 2.

[0061] The test conditions for the fragrance retention effect are as follows: 2 mL of the above mosquito repellent composition is dropped on a sterile cotton ball (0.3 g), and then placed in an open environment to simulate the fragrance retention effect of the mosquito repellent composition on clothes; after 10 hours, 10 testers are selected to score the fragrance retention, and the test results are averaged. The scoring criteria are shown in Table 2. The test results are shown in Table 3.

[0062] The test conditions for fragrance acceptance and fragrance irritation are as follows: 2 mL of the above mosquito repellent composition is dropped on a sterile cotton ball (0.3 g), and then placed in an open environment for 30 minutes, and 10 testers are selected to score the fragrance acceptance and fragrance irritation, and the test results are averaged. The scoring criteria are shown in Table 2. The test results are shown in Table 3.

[0063] The storage stability test conditions are as follows: 50 mL of the above mosquito repellent composition is taken and placed in a closed environment for 7 days, and then the product system is observed to see whether there is stratification. The test results are shown in Table 3.

[0064] Table 2

[0065]

[0066] Table 3

[0067] Fragrance effect Fragrance acceptance Fragrance irritation Storage stability Example 1 9 9 10 No stratification Comparative Example 1 8 9 9 No stratification Comparative Example 2 8 7 8 No stratification Comparative Example 3 7 6 4 No stratification Comparative Example 4 5 5 6 No stratification Comparative Example 5 6 5 6 No stratification Comparative Example 6 4 8 5 No stratification

[0068] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A mosquito repellent composition based on pandan leaf extract, characterized in that: Raw materials, calculated by mass, include: 15-27 parts of pandan leaf extract, 4-8 parts of geranium extract, 2-5 parts of lavender extract, 1-3 parts of peppermint extract, 10-15 parts of grape seed oil and 40-70 parts of solvent.

2. The mosquito repellent composition based on pandan leaf extract according to claim 1, characterized in that: The preparation method of the pandan leaf extract is supercritical carbon dioxide extraction.

3. The mosquito repellent composition based on pandan leaf extract according to claim 2, characterized in that: The supercritical carbon dioxide extraction comprises the following steps: The pandan leaves are crushed to pass through a 25-80 mesh sieve, and then placed in an extraction kettle of a supercritical carbon dioxide extractor for cyclic extraction. The raw material system is then passed into a separation kettle for collection to obtain a pandan leaf extract.

4. The mosquito repellent composition based on pandan leaf extract according to claim 3, characterized in that: The conditions of the cyclic extraction are: extraction pressure of 15-20 MPa, extraction temperature of 40-50° C., entrainer of 70-85 vol % ethanol aqueous solution, and carbon dioxide flow rate of 10-15 L / min.

5. The mosquito repellent composition based on pandan leaf extract according to claim 1, characterized in that: The raw materials are calculated by mass: 26 parts of pandan leaf extract, 7 parts of geranium extract, 3 parts of lavender extract, 2 parts of peppermint extract, 15 parts of grape seed oil and 60 parts of solvent.

6. The mosquito repellent composition based on pandan leaf extract according to claim 1, characterized in that: The solvent is ethanol.

7. A method for preparing the mosquito repellent composition based on pandan leaf extract according to any one of claims 1 to 6, characterized in that: The following steps are involved: The raw materials are mixed to obtain the mosquito repellent composition based on the pandan leaf extract.

8. Use of the mosquito repellent composition based on pandan leaf extract according to any one of claims 1 to 6 in the preparation of products with mosquito repellent effect.

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