Composite pharmaceutical composition, feed additive, snail breeding feed and application of snail breeding feed in improving growth performance of babylonia lutosa

Abstract

The invention discloses a composite pharmaceutical composition, a feed additive, a babylonia breeding feed and application of the babylonia breeding feed in improving the growth performance of babylonia. The composite pharmaceutical composition comprises a traditional Chinese medicine composite extract and growth promoting bacteria in a mass ratio of (800-1200): (20-85) in parts by weight, the traditional Chinese medicine compound extract is prepared from the following raw materials in parts by weight: 10-12 parts of medicated leaven, 8-12 parts of folium cortex eucommiae, 6-8 parts of radix astragali, 6-8 parts of yucca, 5-10 parts of poria cocos, 5-10 parts of rhizoma polygoni cuspidati, 5-10 parts of flos caryophylli, 4-8 parts of semen raphani, 4-8 parts of ramulus uncariae cum uncis, 4-8 parts of folium perillae, 3-5 parts of fructus chaenomelis, 3-6 parts of herba leonuri, 3-6 parts of herba taraxaci, 3-6 parts of rhizoma curcumae longae, 3-7 parts of fructus crataegi, 3-5 parts of folium isatidis and 3-6 parts of fructus hordei germinatus. And 3-6 parts of mulberry leaves. The composite pharmaceutical composition can significantly improve the growth performance of babylonia, and avoids side effects caused by use of chemical drugs such as antibiotics.

Description

Technical Field

[0001] The invention belongs to the technical field of aquatic animal breeding, and more specifically, relates to a composite pharmaceutical composition, a feed additive, a snail breeding feed and use thereof in improving the growth performance of babylonica. Background Art

[0002] Dongfeng conch is a marine shellfish with high economic value. Its meat is delicious and nutritious, and it is deeply loved by consumers. However, in the cultivation process of Dongfeng conch, there are problems such as slow growth, low immunity, and high mortality, which seriously affect the cultivation benefits.

[0003] The main reasons for the slow growth of baby snails are as follows: on the one hand, the nutritional components of existing biological baits are not comprehensive and balanced enough to meet the needs of rapid growth of baby snails; on the other hand, the digestive system of baby snails is relatively fragile, and the digestion and absorption efficiency of baits is not high, which affects the growth rate. Therefore, it is of great practical significance to develop a safe and effective feed additive and feed to improve the growth performance of baby snails.

[0004] Patent Publication No. CN101715884A discloses a powdered feed for Dongfeng snails, including a basic feed, in which the components of the basic feed formula are selected from kelp powder accounting for 5% of the total weight; nano-trace mineral elements equivalent to 0.1-0.15% of the weight of the basic feed are added to the basic feed; Vc polyphosphate, β-glucan, phytase, and oligoxylose are also added to the basic feed. However, the ingredients of this feed additive are relatively single, and the effect of improving the growth performance of Dongfeng snails is limited. Patent Publication No. CN115633744A discloses an artificially expanded feed for Dongfeng snails and a preparation method thereof, wherein the feed includes earthworm extract, nucleotides and Chinese herbal preparations. However, the types and proportions of Chinese herbal preparations in the feed are not optimized enough, and the degree of improvement in the growth performance of Dongfeng snails still needs to be improved. Summary of the invention

[0005] In view of the above-mentioned existing technical problems, the primary purpose of the present invention is to provide a compound pharmaceutical composition, which can significantly improve the growth performance of Babylonia snails while avoiding the side effects caused by the use of chemical drugs such as antibiotics.

[0006] The second object of the present invention is to provide a method for preparing the composite pharmaceutical composition.

[0007] The third object of the present invention is to provide a feed additive.

[0008] The fourth object of the present invention is to provide a snail breeding feed.

[0009] A fifth object of the present invention is to provide a method for preparing snail breeding feed.

[0010] The sixth object of the present invention is to provide a composite pharmaceutical composition, or a feed additive, or a snail breeding feed for use in improving the growth performance of Babylonia snails.

[0011] In order to achieve the above object, the present invention is implemented by the following technical solutions:

[0012] The present invention claims protection for a composite pharmaceutical composition comprising a composite extract of traditional Chinese medicine and growth-promoting bacteria in a mass ratio of 800-1200:20-85;

[0013] The Chinese medicine composite extract is prepared by the following raw materials by weight: 10-12 parts of Shenqu, 8-12 parts of Eucommia ulmoides leaves, 6-8 parts of Astragalus, 6-8 parts of Yucca, 5-10 parts of Poria, 5-10 parts of Polygonum cuspidatum, 5-10 parts of Clove, 4-8 parts of Radish Seed, 4-8 parts of Uncaria rhynchophylla, 4-8 parts of Perilla frutescens leaves, 3-5 parts of Papaya, 3-6 parts of Leonurus japonicus, 3-6 parts of Taraxacum, 3-6 parts of Curcuma longa, 3-7 parts of Hawthorn, 3-5 parts of Isatis indigotica, 3-6 parts of Malt, and 3-6 parts of Mulberry leaves;

[0014] In terms of weight, the growth-promoting bacteria include: 1-2 parts of Clostridium butyricum, 1-2 parts of Bifidobacterium, 1-2 parts of yeast and 1-2 parts of Streptococcus thermophilus.

[0015] The present invention uses a combination of traditional Chinese medicine compound extracts and growth-promoting bacteria to provide a variety of nutrients and functional ingredients, which can synergistically improve the immunity of the baby snails, enhance the palatability of feed, and supplement nutrition, thereby synergistically improving the health of the baby snails and improving their growth performance. Specifically:

[0016] Among the Chinese medicine compound extracts, astragalus, tuckahoe, hawthorn, eucommia leaf, yucca, and malt contain polysaccharides, which have multiple functions such as immune regulation and anti-oxidation. They can provide nutritional support for the snail, promote cell division and growth, and are also a source of energy. These polysaccharide components can participate in sugar metabolism in the snail, and participate in the composition of cells and the regulation of physiological functions.

[0017] Shenqu, malt, astragalus, poria, radish seeds, hawthorn, Uncaria rhynchophylla, Eucommia ulmoides leaves, and yucca are rich in protein and amino acids. Protein is a nutrient necessary for the growth and development of baby snails, and is used to build their own body tissues, such as muscles and shells. Amino acids are the basic units of protein synthesis, and baby snails can use the amino acids in these extracts to synthesize the proteins they need.

[0018] Chinese medicines such as Polygonum cuspidatum, dandelion, Eucommia ulmoides leaf, Uncaria rhynchophylla, Perilla frutescens leaf, mulberry leaf, turmeric, and Isatis indigotica contain bioactive ingredients such as flavonoids and phenols. Flavonoids have antioxidant, anti-inflammatory and immunomodulatory effects. In the body of the baby snail, these ingredients can regulate the activity of immune cells, such as stimulating phagocytes in blood cells, enhancing phagocytic function, and clearing harmful pathogens in the body, such as bacteria and viruses. Phenolic compounds can activate immune-related signaling pathways in the baby snail, induce the expression of immune-related genes, make the baby snail's own immune system more sensitive and efficient, and enhance its resistance to disease.

[0019] Clove, perilla leaf, eucommia leaf, yucca, astragalus, Uncaria rhynchophylla, dandelion, turmeric, and isatis indigotica have natural antibacterial and antiviral ingredients. When the snails come into contact with these extracts, these antibacterial ingredients can form a protective barrier on their body surface and inside their bodies, reducing the attachment and invasion of harmful microorganisms, which is of great significance for preventing viruses from infecting the snails and maintaining their health.

[0020] Among the growth-promoting bacteria, Clostridium butyricum can produce short-chain fatty acids such as butyric acid. Butyric acid is an important energy source for the intestinal cells of Dongfeng snails. It can promote the growth and repair of intestinal cells of Dongfeng snails, improve the absorption function of the intestine, and regulate the balance of the intestinal flora of Dongfeng snails. At the same time, short-chain fatty acids can regulate the pH value of the intestine and create a microenvironment that is conducive to the digestion and absorption of nutrients. Clostridium butyricum can colonize in the intestine and inhibit the growth of harmful bacteria. At the same time, Clostridium butyricum can stimulate the proliferation and activity of immune cells in the intestine of Dongfeng snails, such as lymphocytes.

[0021] Bifidobacteria can also produce organic acids such as lactic acid, which help the snail absorb minerals in the feed, such as calcium and iron. By lowering the pH value of the intestine, minerals exist in the form of ions that are easier to absorb, thereby improving the efficiency of the snail in utilizing nutrients. In addition, bifidobacteria can activate the intestinal immune defense system of the snail, and by interacting with intestinal mucosal cells, induce the secretion of immune substances such as immunoglobulins, enhance the intestinal immune barrier function, and prevent pathogens from invading the snail through the intestine.

[0022] Yeast is rich in protein and amino acids. Yeast synthesizes a large amount of high-quality protein during its growth, which can be broken down into amino acids and absorbed by the snails after they are ingested. In addition, components of yeast cell walls, such as β-glucan, have immunomodulatory effects. It can activate immune cells such as macrophages in the snails, enhance nonspecific immune function, and improve the snails' ability to identify and eliminate pathogens.

[0023] During the fermentation process, thermophilic streptococci also produce some small peptides, which can be directly absorbed by the snails, providing them with a nitrogen source for the synthesis of their own proteins and promoting their growth. In addition, thermophilic streptococci can produce some antibacterial substances, inhibit the growth of harmful microorganisms, and help maintain the stability of the intestinal microecology, indirectly improving the immunity of the snails.

[0024] The present invention combines the traditional Chinese medicine composite extract and the growth-promoting bacteria to achieve a synergistic effect, thereby improving the immunity of the baby snails and significantly improving the growth performance of the baby snails, while avoiding the side effects caused by the use of chemical drugs such as antibiotics.

[0025] Specifically, Shenqu is mainly in the form of square or rectangular blocks, with a yellowish brown and rough appearance; it is hard, brittle and easy to break, with an uneven cross section and is off-white. It is mainly used for food stagnation, indigestion, abdominal distension, loss of appetite, vomiting and diarrhea.

[0026] Eucommia leaves are elliptical, ovate or rectangular, thin and leathery, with a rounded or broad cuneate base, a gradually pointed tip and serrated edges. Eucommia leaves are rich in nutrients, including VB1, VE, β-carotene, 17 free amino acids, and 15 trace elements such as germanium and selenium. The active ingredients of Eucommia leaves include flavonoids, cyclopentane ether terpenes, phenylpropanoids, lignans, polysaccharides and Eucommia rubber. The main pharmacological effects are lowering blood pressure, lowering blood lipids, lowering blood sugar, anti-inflammatory and antiviral, anti-fatigue, strengthening tendons and bones, and anti-aging.

[0027] Astragalus membranaceus (Fisch.) Bunge is a plant of the genus Astragalus in the leguminous family. Astragalus has the effects of tonifying qi and raising yang, consolidating the exterior and stopping sweating, promoting diuresis and reducing swelling, promoting fluid and nourishing blood, relieving stagnation and relieving numbness, expelling toxins and discharging pus, and healing sores and promoting tissue regeneration. It is mainly used to treat all symptoms of qi deficiency and blood deficiency, and can be used to treat qi deficiency and fatigue, poor appetite and loose stools, sinking of qi in the middle, long-term diarrhea and prolapse of the anus, bloody stool and metrorrhagia, spontaneous sweating due to exterior deficiency, edema, internal heat and thirst, blood deficiency and sallow complexion, hemiplegia, pain and numbness, carbuncle that is difficult to heal, long-term ulcer that does not heal, and cough due to lung deficiency, etc.

[0028] Yucca is a plant of the genus Yucca in the family Asparagaceae. It has short or stemless stems, candlestick-shaped panicles, cup-shaped, drooping, white flowers; the leaf base is sword-shaped and radiating, covered with white powder, and leathery.

[0029] Poria cocos is the dried sclerotium of the Polyporaceae fungus Poria cocos (Schw.) Wolf. It is mainly used to treat edema, oliguria, phlegm and fluid, dizziness and palpitations, spleen deficiency, poor appetite, loose stools, restlessness, palpitations and insomnia.

[0030] Polygonum cuspidatum (scientific name: Reynoutria japonica Houtt.), also known as spotted root, big bone setting, sour barrel reed, and sour barrel rod, is a perennial herbaceous plant of the Polygonum cuspidatum genus of the Polygonaceae family. Polygonum cuspidatum has the effects of removing rheumatism and detoxification, and is mainly used to treat rheumatic joint pain and snake bites; it has the effects of promoting blood circulation and removing blood stasis, promoting dampness and relieving jaundice, and clearing away heat and detoxification.

[0031] Syringa oblata Lindl., also known as white lilac, hairy lilac, North China lilac, and lilac, is a plant of the genus Syringa in the family Oleaceae. Lilac is a shrub or small tree that can reach 5 meters in height; the bark is gray-brown or gray. The twigs, inflorescence axis, pedicel, bract, calyx, both sides of young leaves, and petioles are hairless and densely covered with glandular hairs. Lilac has the effects of clearing heat, detoxifying, promoting dampness, and relieving jaundice, and can treat acute diarrhea, icteric hepatitis, conjunctivitis, and other diseases.

[0032] Radish seeds are the dried mature seeds of the cruciferous plant Raphanus sativus L. They are mainly used for food stagnation, abdominal distension and pain, constipation, stagnation of stool, diarrhea, phlegm and cough.

[0033] Uncariarhynchophylla (Miq.) Miq.ex Havil., a vine of the Rubiaceae family, has the effects of clearing blood and calming the liver, calming wind and calming convulsions, and is used to treat wind-heat headaches, colds and convulsions, and the rhynchophylline it contains has the effect of lowering blood pressure.

[0034] Perilla leaf is the leaf of Perilla frutescens (L.) Britt., an annual upright herbaceous plant of the genus Perilla in the family Lamiaceae, with green or purple stems, blunt quadrangular; leaves broadly ovate or round, with rounded or broadly cuneate bases; bracts broadly ovate or nearly round, glabrous; calyx bell-shaped. Perilla has the effects of dispelling cold, promoting qi and relieving fullness, and calming the fetus, and is mainly used to treat symptoms such as colds, chest tightness and vomiting, and restless fetuses.

[0035] Papaya (Pseudocydonia sinensis (Dum.Cours.)CKSchneid.) is the only species of the genus Papaya in the Rosaceae family. It is also known as smooth-skinned papaya, crabapple, and wood plum. Papaya has the effects of soothing the stomach and relaxing the muscles, dispelling rheumatism, relieving cough and reducing phlegm; it is mainly used to treat vomiting, diarrhea, cramps, rheumatism, cough and sputum, diarrhea, dysentery, injuries from falls, athlete's foot, edema, etc.

[0036] Motherwort (Leonurus japonicus Houtt.) is an annual or biennial herb belonging to the genus Leonurus of the family Lamiaceae. It has dense fibrous roots on its roots. Its stem is erect and obtusely quadrangular. The leaves at the bottom of the stem are ovate, and the leaves in the middle of the stem are rhombus-shaped. The leaves are alternate and opposite, with petioles. Motherwort has the effects of promoting blood circulation and removing blood stasis, regulating menstruation and eliminating water retention. It can treat irregular menstruation, dystocia, retained placenta, abdominal pain caused by blood stasis after childbirth, metrorrhagia, hematuria, diarrhea, pain, swelling and ulcers.

[0037] Dandelion (scientific name: Taraxacum mongolicum Hand.-Mazz.) is a perennial herbaceous plant of the genus Taraxacum in the Asteraceae family. It has the effects of clearing away heat and detoxifying, reducing swelling and dispersing knots, and promoting diuresis and relieving stranguria. It can be used to treat carbuncle swelling, breast carbuncle, scrofula, red eyes, sore throat, lung carbuncle, intestinal carbuncle, damp-heat jaundice, hot stranguria and other symptoms.

[0038] Hawthorn (scientific name: Crataegus pinnatifida Bunge) is a deciduous tree of the genus Crataegus in the Rosaceae family. Hawthorn has the effect of eliminating food stagnation and removing blood stasis, and is mainly used to treat food stagnation, abdominal distension and pain, etc.; its flowers have the effect of lowering blood pressure, and are mainly used to treat hypertension.

[0040] Folium Isatidis is a plant of the genus Isatis in the family Cruciferae. It is used for fever, high fever, thirst, coma, rashes, hematemesis, epistaxis, jaundice, diarrhea, erysipelas, throat congestion, mouth ulcers, and mumps.

[0041] Malt is a plant of the genus barley in the family Poaceae. It can also lower blood sugar, inhibit the release of prolactin, and fight colitis. It is mainly used to promote qi and aid digestion, strengthen the spleen and appetite, and relieve milk production and bloating.

[0042] Mulberry leaves (Morus alba Linn) can improve the production performance of animals, improve the antioxidant capacity of animals, and improve the balance of intestinal microecology of animals.

[0043] Preferably, the mass ratio of the traditional Chinese medicine composite extract to the growth-promoting bacteria is 900-1100:40-60.

[0044] Furthermore, the present invention claims a method for preparing a composite pharmaceutical composition, comprising the following steps:

[0045] S1. extracting the raw material of the Chinese medicine composite extract by alcohol extraction, and post-processing to obtain the Chinese medicine composite extract;

[0046] S2. Evenly mix the traditional Chinese medicine compound extract and the growth-promoting bacteria, and ferment to obtain a compound pharmaceutical composition.

[0047] Preferably, the conditions of the above preparation method are selected from any one of the following (a)-(e):

[0048] (a) the mass ratio of the raw material to the alcohol solvent is 1:1.5-2;

[0049] (b) the extraction temperature of the alcohol extraction method is 50-70°C;

[0050] (c) the extraction is ultrasonic extraction;

[0051] (d) the power of the ultrasonic extraction is 100-500W, and the time of the ultrasonic extraction is 20-40min;

[0052] (e) The fermentation time is 30-50 days.

[0053] Preferably, in step S1, the raw material of the Chinese medicine composite extract is first crushed before being extracted by alcohol extraction. The crushing process makes the raw material easier to extract later. More preferably, the particle size of the raw material after the crushing process is 60-200 mesh.

[0054] Preferably, in step S1, the post-treatment is to concentrate the extract to remove the alcohol solvent.

[0055] Preferably, in step S1, the alcohol solvent is ethanol.

[0056] Preferably, in step S1, the extraction is performed 1-3 times.

[0057] Furthermore, the present invention claims protection for a feed additive, comprising a basic base material, a seaweed extract, a Chinese medicine compound extract and growth-promoting bacteria; the mass ratio of the basic base material, the seaweed extract, the Chinese medicine compound extract and the growth-promoting bacteria is 500-650:300-400:800-1200:20-85; the Chinese medicine compound extract is prepared by the following raw materials in parts by weight: 10-12 parts of Shenqu, 8-12 parts of Eucommia ulmoides leaves, 6-8 parts of Astragalus membranaceus, 6 parts of Yucca schrenkiana -8 parts, Poria cocos 5-10 parts, Polygonum cuspidatum 5-10 parts, Clove 5-10 parts, Radish seed 4-8 parts, Uncaria rhynchophylla 4-8 parts, Perilla leaf 4-8 parts, Papaya 3-5 parts, Leonurus japonicus 3-6 parts, Taraxacum officinale 3-6 parts, Curcuma longa 3-6 parts, Hawthorn 3-7 parts, Isatis indigotica 3-5 parts, Malt 3-6 parts, Mulberry leaf 3-6 parts; by weight, the growth-promoting bacteria include: 1-2 parts of Clostridium butyricum, 1-2 parts of Bifidobacterium, 1-2 parts of Saccharomyces cerevisiae and 1-2 parts of Streptococcus thermophilus.

[0058] Preferably, the seaweed extract is prepared from the following raw materials: Sargassum, Ulva, Laminaria japonica and Gracilaria lemaneiformis.

[0059] Preferably, the mass ratio of the Sargassum, Ulva, Laminaria and Gracilaria is 3-5:1-2:2-3:2-3.

[0060] Preferably, the basic base material includes one or more of scallop meat, five-grain worms, artemia, squid paste, brewer's yeast, and coconut meat.

[0061] Preferably, in terms of weight, the basic base material includes 10-15 parts of scallop meat, 8-14 parts of five-grain worms, 4-9 parts of artemia, 2-5 parts of cuttlefish paste, 1-2 parts of brewer's yeast, and 5-7 parts of coconut meat.

[0062] In the present invention, the basic base material includes scallop meat, squid paste, grain worms, brewer's yeast, grain worms and brewer's yeast. The basic base material is a high-protein food source, and its protein contains various essential amino acids required by the baby snail, which are the basis for building its own tissue protein during the growth and development of the baby snail. Amino acids such as arginine and histidine are essential for the muscle growth and repair of the baby snail. After eating scallop meat, the baby snail can use the protein therein to build and maintain its own muscle tissue, enhance its athletic ability, and help to find food in the water body, avoid natural enemies and other activities. Rich in protein, and its amino acid composition is relatively balanced. These proteins can be decomposed into small molecular peptides and amino acids by the baby snail digestive enzyme, and then absorbed into the body. These absorbed nutrients are used to synthesize various functional proteins in the baby snail, such as enzymes, hormones, etc. Enzymes participate in the metabolism of the baby snail, including digestion of food, synthesis of shells and other processes; hormones regulate the growth, development, reproduction and other physiological processes of the baby snail. This protein has a significant promoting effect on the growth of the baby snail.

[0063] In addition to containing a large amount of high-quality protein, brewer's yeast is also rich in B vitamins. After the snail ingests brewer's yeast, it can not only obtain protein for its own growth, but also use the B vitamins in it to participate in energy metabolism, provide energy for the snail, and help maintain its normal physiological activities.

[0064] The basic base material is also rich in trace elements such as calcium, phosphorus, iron, and zinc. Calcium is a key element for the growth of the shell of the Dongfeng snail. The Dongfeng snail can absorb calcium from scallop meat and squid paste and transport it to the shell formation site for the growth and strengthening of the shell. Phosphorus and calcium jointly participate in the mineralization process of bones (shells) to ensure the hardness and toughness of the shells. Trace elements such as iron and zinc participate in the composition and activation of various enzymes in the Dongfeng snail, which helps the digestion and immune function of the Dongfeng snail. Scallop meat and squid paste have a strong seafood flavor. This natural umami taste can increase the Dongfeng snail's preference for the basic base material and increase its food intake. Compared with feeds without these umami substances, Dongfeng snails are more inclined to eat base materials containing scallop meat and squid paste, so as to better absorb the nutrients therein and promote their own growth and development.

[0065] Among seaweed extracts, seaweeds such as Sargassum and kelp are rich in polysaccharides, such as alginate and kelp polysaccharides. These polysaccharides are an important source of energy for the snail. They can be partially decomposed into monosaccharides in the snail, and then participate in the energy metabolism process to provide energy for the life activities of the snail. At the same time, seaweed polysaccharides can also serve as a dietary fiber to promote the peristalsis of the snail's intestines. In the intestine, polysaccharides can absorb some harmful substances, such as heavy metal ions, etc., to reduce the harm of these harmful substances to the snail. In addition, seaweed polysaccharides can be used by intestinal microorganisms to produce some short-chain fatty acids, which can be absorbed and utilized by the snail to provide it with additional energy and nutrition. Seaweed contains a variety of vitamins, such as vitamin A, vitamin C, etc. These vitamins can supplement the lack of vitamins obtained by the snail from other foods, improve its overall nutritional level, and promote its healthy growth.

[0066] Seaweeds such as Ulva and Gracilaria are rich in minerals, such as calcium, magnesium, and iron. Calcium is a key element for the growth of the shells of the baby snails. The calcium in the seaweed extract can be absorbed and utilized by the baby snails to supplement their calcium needs during the growth of the shells. Magnesium participates in the activation of multiple enzymes in the baby snails and plays an important role in the metabolism of the baby snails. These minerals can supplement the baby snails' needs for minerals during their growth and maintain their normal physiological functions and growth and development.

[0067] Some active ingredients in seaweed extracts, such as fucoxanthin, have antioxidant and immunomodulatory effects. In the body of the snail, fucoxanthin can activate immune-related cells, such as phagocytes in blood cells, and enhance its ability to phagocytize pathogens. Sulfated polysaccharides and other ingredients in seaweed can also regulate the immune system of the snail. They can activate immune signaling pathways, induce the expression of immune-related genes in the snail, make the snail's immune system more complete and efficient, and enhance its resistance to disease. Some ingredients in seaweed extracts have antibacterial and antiviral properties. For example, phenolic compounds in some seaweeds can inhibit the growth and reproduction of bacteria. When the snail comes into contact with these extracts, these antibacterial ingredients can form a protective barrier on its body surface and inside the body, reducing the attachment and invasion of harmful microorganisms. For viruses, some ingredients in seaweed extracts may interfere with the adsorption, invasion or replication of viruses, thereby reducing the risk of viral infection in the snail and maintaining its health.

[0068] Furthermore, the present invention claims a method for preparing a feed additive, comprising the following steps:

[0069] (1) extracting the raw material of the Chinese herbal medicine composite extract by alcohol extraction, and post-processing to obtain the Chinese herbal medicine composite extract;

[0070] (2) mixing scallop meat, five-grain worms, Artemia worms, cuttlefish paste, brewer's yeast, and coconut meat uniformly, and crushing to obtain a basic base material;

[0071] (3) extracting the raw material of the seaweed extract by an alcohol extraction method, and post-processing to obtain the seaweed extract;

[0072] (4) mixing Clostridium butyricum, Bifidobacterium, yeast and Streptococcus thermophilus to obtain growth-promoting bacteria;

[0073] (5) The Chinese herbal compound extract, basic base material, seaweed extract and growth-promoting bacteria are mixed and fermented to obtain the feed additive.

[0074] Preferably, in step (1), the mass ratio of the raw material of the Chinese herbal compound extract to the alcohol solvent is 1:1.5-2.

[0075] Preferably, in step (1), the extraction temperature of the alcohol extraction method is 50-70°C.

[0076] Preferably, in step (1), the extraction is ultrasonic extraction. Further preferably, the power of the ultrasonic extraction is 100-500W, and the time of the ultrasonic extraction is 20-40min.

[0077] Preferably, in step (2), the particle size of the crushed base material is 60-120 meshes.

[0078] Preferably, in step (3), the mass ratio of the raw material of the seaweed extract to the alcohol solvent is 1:1.5-2.

[0079] Preferably, in step (3), the temperature of the alcohol extraction is 50-70°C.

[0080] Preferably, in step (3), the extraction is ultrasonic extraction. Further preferably, the power of the ultrasonic extraction is 100-500W, and the time of the ultrasonic extraction is 20-40min.

[0081] Preferably, in step (1) and step (3), the alcohol solvent is ethanol.

[0082] Preferably, in step (1) and step (3), the extraction is performed 1 to 3 times.

[0083] Preferably, in step (5), the fermentation time is 30-50 days.

[0084] Furthermore, the present invention claims protection for a snail breeding feed, comprising:

[0085] (a) 0.5-10.0% by weight of the composite pharmaceutical composition or the feed additive;

[0086] (b) Basic feed.

[0087] Specifically, the snail breeding feed comprises: (a) 0.8-2.5% by weight of the compound pharmaceutical composition or the feed additive; and (b) basic feed.

[0088] Preferably, in terms of weight, the basic feed includes 4-6 parts of diatom mud, 1-3 parts of vitamin D, 0.5-1 parts of copper sulfate, 0.5-1 parts of amino acid chelated zinc, 20-40 parts of gluten powder, and 50-70 parts of fish meal.

[0089] Furthermore, the present invention seeks to protect a method for preparing a snail breeding feed, comprising mixing the composite pharmaceutical composition or the feed additive with a basic feed, performing puffing and drying treatments, and obtaining the snail breeding feed.

[0090] Preferably, the temperature of the puffing treatment is 100-120°C.

[0091] Preferably, the temperature of the drying treatment is 55-75°C.

[0092] Specifically, the snail breeding feed prepared by the present invention has a moisture content of ≤30%, is in a soft cake or sheet shape, and is in a semi-suspended state when put into water.

[0093] Furthermore, the present invention seeks to protect a method for breeding Babylonia snails, which uses the composite pharmaceutical composition, or the feed additive, or the snail breeding feed to breed Babylonia snails.

[0094] Preferably, the compound pharmaceutical composition, or the feed additive, or the snail breeding feed is mixed with bait and fed to the babylonia snails. The amount of bait fed accounts for 8-15% of the weight of the babylonia snails. Further preferably, the amount of bait fed accounts for 10-12% of the weight of the babylonia snails.

[0095] Preferably, in the first 1-2 months, 8-15 g of the compound pharmaceutical composition is added per kilogram of bait; in the third 1-6 months, 16-20 g of the compound pharmaceutical composition is added per kilogram of bait fish; in the seventh 1-8 months, 22-30 g of the compound pharmaceutical composition is added per kilogram of bait fish.

[0096] Furthermore, the present invention seeks to protect the use of the composite pharmaceutical composition, or the feed additive, or the snail breeding feed in improving the growth performance of Babylonia snails.

[0097] Specifically, the growth performance of the Babylonia paniculata refers to: improving the specifications of the Babylonia paniculata.

[0098] Specifically, the babylonia is of the genus Babylonia (Babylonia), including but not limited to Babylonia areolata, Babylonia lutosa, Babylonia formosae, etc.

[0099] Compared with the prior art, the present invention has the following beneficial effects:

[0100] (1) The present invention provides a compound pharmaceutical composition, which can provide a variety of nutrients and functional ingredients, can synergistically improve the immunity of the baby snail, enhance the palatability of the feed, supplement nutrition, and can synergistically improve the health status of the baby snail and improve the growth performance of the baby snail.

[0101] (2) The present invention further provides a feed additive and a snail breeding feed on the basis of the composite pharmaceutical composition. The feed additive and the snail breeding feed can provide a variety of nutrients and functional ingredients, can improve the digestibility, absorption rate, nutritional value and functionality of the feed, and further improve the growth performance of the baby snail.

[0102] Instruction Manual

[0103] Figure 1 The small snail breeding feed prepared in Example 7.

[0104] Figure 2 The large snail breeding feed prepared in Example 7.

[0105] Figure 3 This is a status diagram of the first month of the Babylonia snail feeding experiment in Example 10.

[0106] Figure 4 This is a status diagram of the fourth month of the Babylonia snail feeding experiment in Example 10.

[0107] Figure 5 This is a status diagram of the 7th month of the Babylonia snail feeding experiment in Example 10.

[0108] Figure 6 This is a status diagram of the breeding ponds for multiple groups of Babylonia snail feeding experiments. DETAILED DESCRIPTION

[0109] The present invention is further described below in conjunction with the accompanying drawings and specific examples, but the examples do not limit the present invention in any form. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art.

[0110] In the following examples, Clostridium butyricum (preservation number: CGMCC 1.5205); Bifidobacterium (preservation number: CGMCC1.5090); Saccharomyces cerevisiae (preservation number: CGMCC 2.5692); Streptococcus thermophilus (preservation number: CGMCC 1.8748) were purchased from the China Microbial Culture Resource Bank.

[0111] Example 1 Compound Pharmaceutical Composition

[0112] (1) 12 parts of Shenqu, 10 parts of Eucommia ulmoides leaves, 6 parts of Astragalus, 6 parts of Yucca, 8 parts of Poria, 8 parts of Polygonum cuspidatum, 8 parts of Clove, 4 parts of Radish Seed, 4 parts of Uncaria rhynchophylla, 6 parts of Perilla leaves, 3 parts of Papaya, 5 parts of Leonurus japonicus, 5 parts of Taraxacum, 5 parts of Curcuma longa, 3 parts of Crataegus, 4 parts of Isatis indigotica, 5 parts of Malt, and 3 parts of Morus alba were blended and mixed with 1.5 times volume of ethanol, ultrasonically treated for 30 min at a power of 300 W, filtered, and the filtrate was concentrated to remove ethanol. The concentrated filtrate was the Chinese medicine composite extract.

[0113] (2) Weigh 2 parts of Clostridium butyricum, 2 parts of Bifidobacterium, 1 part of Saccharomyces cerevisiae and 2 parts of Streptococcus thermophilus according to weight, mix them evenly, and obtain growth-promoting bacteria.

[0114] (3) The Chinese medicine composite extract and the growth-promoting bacteria are poured into a blender at a mass ratio of 1000:50 and mixed evenly, and then poured into a fermentation tank and fermented for 40 days to obtain a composite pharmaceutical composition.

[0115] Example 2 Compound Pharmaceutical Composition

[0116] (1) 12 parts of Shenqu, 12 parts of Eucommia ulmoides leaves, 8 parts of Astragalus, 8 parts of Yucca, 10 parts of Poria, 10 parts of Polygonum cuspidatum, 10 parts of Clove, 8 parts of Radish Seed, 8 parts of Uncaria rhynchophylla, 8 parts of Perilla leaves, 5 parts of Papaya, 6 parts of Leonurus japonicus, 6 parts of Taraxacum, 6 parts of Curcuma longa, 7 parts of Crataegus, 5 parts of Isatis indigotica, 6 parts of Malt, and 6 parts of Morus alba were blended and mixed with 2 times volume of ethanol, ultrasonically treated for 40 min at a power of 500 W, filtered, and the filtrate was concentrated to remove ethanol. The concentrated filtrate was the Chinese medicine composite extract.

[0117] (2) Weigh 2 parts of Clostridium butyricum, 2 parts of Bifidobacterium, 2 parts of yeast and 1.5 parts of Streptococcus thermophilus according to weight, mix them evenly, and obtain growth-promoting bacteria.

[0118] (3) The Chinese medicine composite extract and the growth-promoting bacteria were poured into a blender at a mass ratio of 900:80 and mixed evenly, and then poured into a fermentation tank and fermented for 50 days to obtain a composite pharmaceutical composition.

[0119] Example 3 Compound Pharmaceutical Composition

[0120] (1) 10 parts of Shenqu, 8 parts of Eucommia ulmoides leaves, 7 parts of Astragalus, 7 parts of Yucca, 5 parts of Poria, 5 parts of Polygonum cuspidatum, 5 parts of Clove, 6 parts of Radish Seed, 5 parts of Uncaria rhynchophylla, 4 parts of Perilla leaves, 4 parts of Papaya, 3 parts of Leonurus japonicus, 3 parts of Taraxacum, 3 parts of Curcuma longa, 5 parts of Crataegus, 3 parts of Isatis indigotica, 3 parts of Malt, and 6 parts of Morus alba were blended and mixed with 2 times volume of ethanol, ultrasonically treated for 40 min at a power of 500 W, filtered, and the filtrate was concentrated to remove ethanol. The concentrated filtrate was the Chinese medicine composite extract.

[0121] (2) Weigh 2 parts of Clostridium butyricum, 2 parts of Bifidobacterium, 2 parts of yeast and 1.5 parts of Streptococcus thermophilus according to weight, mix them evenly, and obtain growth-promoting bacteria.

[0122] (3) The Chinese medicine composite extract and the growth-promoting bacteria were poured into a blender at a mass ratio of 900:80 and mixed evenly, and then poured into a fermentation tank and fermented for 50 days to obtain a composite pharmaceutical composition.

[0123] Example 4 Feed Additive

[0124] (1) According to the proportion, 10 parts of scallop meat, 8 parts of five-grain worms, 5 parts of Artemia worms, 4 parts of cuttlefish paste, 1 part of brewer's yeast, and 5 parts of coconut meat are weighed and mixed evenly, and ultrafinely ground to 100 mesh to obtain a basic base material.

[0125] (2) 12 parts of Shenqu, 10 parts of Eucommia ulmoides leaves, 6 parts of Astragalus, 6 parts of Yucca, 8 parts of Poria, 8 parts of Polygonum cuspidatum, 8 parts of Clove, 4 parts of Radish Seed, 4 parts of Uncaria rhynchophylla, 6 parts of Perilla leaves, 3 parts of Papaya, 5 parts of Leonurus japonicus, 5 parts of Taraxacum, 5 parts of Curcuma longa, 3 parts of Crataegus, 4 parts of Isatis indigotica, 5 parts of Malt, and 3 parts of Morus alba were blended and mixed with 1.5 times volume of ethanol, extracted at 65°C, ultrasonically treated for 30 min at a power of 300 W, filtered, and the filtrate was concentrated to remove ethanol, extracted once, and the concentrated filtrate was the Chinese medicine composite extract.

[0126] (3) Chop Sargassum, Ulva, Laminaria japonica, and Gracilaria lemaneiformis in a weight ratio of 5:1:3:2, mix with 1.5 times the volume of ethanol, extract at 65°C, ultrasonically treat for 40 min at a power of 300 W, filter, extract the filtrate and concentrate to remove ethanol, extract once, and the concentrated filtrate is the seaweed extract.

[0127] (4) Weigh 2 parts of Clostridium butyricum, 2 parts of Bifidobacterium, 1 part of Saccharomyces cerevisiae and 2 parts of Streptococcus thermophilus according to weight, mix them evenly, and obtain growth-promoting bacteria.

[0128] (5) The basic base material, seaweed extract, Chinese medicine compound extract and growth-promoting bacteria are poured into a blender at a mass ratio of 600:300:1000:50 and mixed evenly, and then poured into a fermentation tank for fermentation for 40 days to obtain a feed additive.

[0129] Example 5 Feed Additive

[0130] (1) According to the proportion, 13 parts of scallop meat, 12 parts of five-grain worms, 7 parts of Artemia, 2 parts of cuttlefish paste, 2 parts of brewer's yeast, and 5 parts of coconut meat are weighed and mixed evenly, and ultrafinely ground to 100 mesh to obtain a basic base material.

[0131] (2) 12 parts of Shenqu, 12 parts of Eucommia ulmoides leaves, 8 parts of Astragalus, 8 parts of Yucca, 10 parts of Poria, 10 parts of Polygonum cuspidatum, 10 parts of Clove, 8 parts of Radish Seed, 8 parts of Uncaria rhynchophylla, 8 parts of Perilla leaves, 5 parts of Papaya, 6 parts of Leonurus japonicus, 6 parts of Taraxacum, 6 parts of Curcuma longa, 7 parts of Crataegus, 5 parts of Isatis indigotica, 6 parts of Malt, and 6 parts of Morus alba were blended and mixed with 2 times the volume of ethanol, ultrasonically treated for 40 min at a power of 500 W, filtered, and the filtrate was concentrated to remove ethanol. The concentrated filtrate was the Chinese medicine composite extract.

[0132] (3) Chop Sargassum, Ulva, Laminaria japonica, and Gracilaria lemaneiformis in a weight ratio of 5:2:3:3, mix with 2 volumes of ethanol, and ultrasonically treat for 40 min at a power of 500 W. Filter, extract the filtrate, and concentrate to remove the ethanol. The concentrated filtrate is the seaweed extract.

[0133] (4) Weigh 2 parts of Clostridium butyricum, 2 parts of Bifidobacterium, 2 parts of yeast and 1.5 parts of Streptococcus thermophilus according to weight, mix them evenly, and obtain growth-promoting bacteria.

[0134] (5) The basic base material, seaweed extract, Chinese medicine compound extract and growth-promoting bacteria are poured into a blender at a mass ratio of 550:350:900:80 and mixed evenly, and then poured into a fermentation tank and fermented for 50 days to obtain a feed additive.

[0135] Example 6 Feed Additive

[0136] (1) According to the proportion, 15 parts of scallop meat, 14 parts of grain worms, 9 parts of Artemia, 5 parts of cuttlefish paste, 1 part of brewer's yeast, and 7 parts of coconut meat are weighed and mixed evenly, and ultrafinely ground to 100 mesh to obtain a basic base material.

[0137] (2) 10 parts of Shenqu, 8 parts of Eucommia ulmoides leaves, 7 parts of Astragalus, 7 parts of Yucca, 5 parts of Poria, 5 parts of Polygonum cuspidatum, 5 parts of Clove, 6 parts of Radish Seed, 5 parts of Uncaria rhynchophylla, 4 parts of Perilla leaves, 4 parts of Papaya, 3 parts of Leonurus japonicus, 3 parts of Taraxacum, 3 parts of Curcuma longa, 5 parts of Crataegus, 3 parts of Isatis indigotica, 3 parts of Malt, and 6 parts of Morus alba were blended and mixed with 2 times volume of ethanol, ultrasonically treated for 40 min at a power of 500 W, filtered, and the filtrate was concentrated to remove ethanol. The concentrated filtrate was the Chinese medicine composite extract.

[0138] (3) Chop Sargassum, Ulva, Laminaria japonica, and Gracilaria lemaneiformis in a weight ratio of 5:2:3:3, mix with 2 volumes of ethanol, and ultrasonically treat for 40 min at a power of 500 W. Filter, extract the filtrate, and concentrate to remove the ethanol. The concentrated filtrate is the seaweed extract.

[0139] (4) Weigh 2 parts of Clostridium butyricum, 2 parts of Bifidobacterium, 2 parts of yeast and 1.5 parts of Streptococcus thermophilus according to weight, mix them evenly, and obtain growth-promoting bacteria.

[0140] (5) The basic base material, seaweed extract, Chinese medicine compound extract and growth-promoting bacteria are poured into a blender at a mass ratio of 550:350:900:80 and mixed evenly, and then poured into a fermentation tank and fermented for 50 days to obtain a feed additive.

[0141] Example 7 Snail breeding feed

[0142] According to the mass percentage, 4 parts of diatom mud, 2 parts of vitamin D, 1 part of copper sulfate, 1 part of amino acid chelated zinc, 30 parts of gluten powder, and 60 parts of fish meal were taken to form a basic feed, and 2.0% of the feed additive prepared in Example 4 was added. After being fully mixed, the mixture was sliced, and then puffed at a temperature of 100° C. and dried at a temperature of 60° C. to obtain a snail breeding feed (such as Figure 1 and Figure 2 As shown in the figure, they are small snail farming feed and large snail farming feed, the difference lies in the different specifications). The moisture content of the snail farming feed is less than 30%, and it is in a soft cake or flake state, and is in a semi-suspended state when put into water.

[0143] Example 8 Snail breeding feed

[0144] According to the mass percentage, 6 parts of diatom mud, 3 parts of vitamin D, 1 part of copper sulfate, 1 part of amino acid chelated zinc, 40 parts of gluten powder, and 70 parts of fish meal are taken to form a basic feed, and then 2.0% of the feed additive prepared in Example 5 is added, and after being fully mixed, sliced, and then puffed at a temperature of 100° C. and dried at a temperature of 60° C., snail breeding feed is obtained. The moisture content of the snail breeding feed is less than 30%, and it is in a soft cake or sheet shape, and is in a semi-suspended state when placed in water.

[0145] Example 9 Snail breeding feed

[0146] According to the mass percentage, 5 parts of diatom mud, 1 part of vitamin D, 0.5 parts of copper sulfate, 0.5 parts of amino acid chelated zinc, 20 parts of gluten powder, and 50 parts of fish meal are taken to form a basic feed, and then 2.0% of the feed additive prepared in Example 6 is added, and after being fully mixed, sliced, and then puffed at a temperature of 100° C. and dried at a temperature of 60° C., snail breeding feed is obtained. The moisture content of the snail breeding feed is less than 30%, and it is in a soft cake or sheet shape, and is in a semi-suspended state when placed in water.

[0147] Example 10 Babylonia conch feeding experiment

[0148] In Hainan Wengtian Farm, cement pools are used as breeding pools. The area of ​​the cement pool is 16m 2 The pool is 0.5m deep and is equipped with water inlet pipes, drainage pipes and air pipes. A black sunshade net should be laid above the breeding pool to prevent excessive light from affecting the growth of Babylonia square-spotted. A sand layer should be laid on the bottom of the breeding pool. In order to facilitate sewage discharge and cleaning of the sand layer, a layer of wooden boards should be laid on the bottom of the pool first, covered with 80-mesh sieve silk, and then laid with sand. Before breeding, the sand and cement pools laid on the bottom of the pool should be disinfected with potassium permanganate, rinsed clean, and then breeding can be carried out.

[0149] The experimental subjects were 2000 Babylonia scabra with an initial weight of 2000 heads / catties purchased from the nursery, and 20,000 Babylonia scabra were placed in each breeding pond. The breeding pond was kept in a 24-hour water flow state, and the dissolved oxygen in the water should be guaranteed to be above 4.0mq / L. The daily water flow was 8 to 12 times the volume of the breeding pond water, and the water temperature was 20℃ to 28℃ during the breeding period.

[0150] The composite drug composition prepared in Example 1 was added to bait fish and fed to Babylonia areolata once every two days, with the feeding amount accounting for 10% of the weight of Babylonia areolata. In the first and second months, 10 g of the composite drug composition was added per kilogram of bait fish. The breeding conditions in the first month were as follows: Figure 3 In the 3rd to 6th months, 18g of the compound drug composition was added per kilogram of bait fish. The breeding conditions in the 4th month were as follows: Figure 4 In the 7th and 8th months, 25 g of the compound drug composition was added per kilogram of bait fish. The breeding conditions in the 7th month were as follows: Figure 5 After continuous feeding for 8 months, commercial snails were harvested.

[0151] Example 11-18 Babylonia snail feeding experiment

[0152] The differences between Examples 11-18 and Example 10 are:

[0153] Example 11 and Example 12 respectively adopt the compound pharmaceutical composition of Example 2 and Example 3.

[0154] In Examples 13, 14 and 15, the feed additives of Examples 4, 5 and 6 were used to replace the compound drug composition. In Examples 13-15, the amount of the compound drug composition added to the bait in the feed additive was consistent with that in Example 10. Figure 6 shown.

[0155] In Examples 16, 17 and 18, the snail farming feed of Examples 7, 8 and 9 are used to replace the compound pharmaceutical composition. In Examples 16-18, the amount of the compound pharmaceutical composition in the feed additive added to the bait is the same as that in Example 10.

[0156] Embodiment 19

[0157] The difference between this embodiment and embodiment 10 is that the growth-promoting bacteria in the composite pharmaceutical composition of embodiment 1 only contain bifidobacteria.

[0158] Embodiment 20

[0159] The difference between this embodiment and embodiment 10 is that the growth-promoting bacteria in the composite pharmaceutical composition of embodiment 1 only contain Clostridium butyricum.

[0160] Embodiment 21

[0161] The difference between this comparative example and Example 16 is that the snail breeding feed in Example 7 does not contain diatom mud.

[0162] Embodiment 22

[0163] The difference between this comparative example and Example 16 is that the snail breeding feed in Example 7 does not contain vitamin D.

[0164] Embodiment 23

[0165] The difference between this comparative example and Example 16 is that the snail breeding feed in Example 7 does not contain copper sulfate and amino acid chelated zinc.

[0166] Comparative Example 1

[0167] The difference between this comparative example and Example 10 is that the composite pharmaceutical composition prepared in Example 1 was not used.

[0168] Comparative Example 2

[0169] The difference between this comparative example and Example 10 is that the composite pharmaceutical composition of Example 1 does not contain the composite extract of traditional Chinese medicine.

[0170] Comparative Example 3

[0171] The difference between this comparative example and Example 10 is that the composite pharmaceutical composition of Example 1 does not contain growth-promoting bacteria.

[0172] Test Case

[0173] Prepare plastic baskets, shovels, seawater sources, screening machines, electronic scales and other equipment in advance. Carefully open the drain of the breeding pond and gradually drain the water in the breeding pond. Use a shovel to shovel out a piece of open space at a suitable location in the breeding pond, and stack two plastic baskets upside down on the open space. Pick up the shovel, carefully scoop up the Dongfeng snails, and gently put them into the upper plastic basket. Use seawater to repeatedly rinse the Dongfeng snails in the plastic basket, allowing the seawater to continuously wash the surface of the snails so that the sand gradually falls off. Shake the plastic basket during the rinsing process to help the sand separate faster. Continue rinsing until the sand on the surface of the Dongfeng snails is basically rinsed clean. Place the washed Dongfeng snails on the screening machine and start the screening machine. The screening machine screens the Dongfeng snails into two different specifications according to the set specifications. Prepare an electronic scale and weigh the two specifications of Dongfeng snails screened out from each breeding pool on the electronic scale. Read the value displayed on the electronic scale and record it. When recording, indicate the specifications and pool numbers for subsequent statistics and analysis.

[0174] Table 1

[0175] Specifications of Dongfeng Conch: 40-50 pieces / catties Example 10 421 Embodiment 11 429 Example 12 420 Embodiment 13 448 Embodiment 14 455 Embodiment 15 444 Example 16 486 Embodiment 17 473 Embodiment 18 476 Embodiment 19 411 Embodiment 20 406 Embodiment 21 463 Embodiment 22 460 Embodiment 23 458 Comparative Example 1 224 Comparative Example 2 357 Comparative Example 3 342

[0176] It can be seen from the above-mentioned Examples 10-23 that the composite pharmaceutical composition, feed additives and snail breeding feed provided by the present invention can provide a variety of nutrients and functional ingredients for Dongfeng Snail, can improve the immunity of Dongfeng Snail, improve the growth performance of Dongfeng Snail, and its specifications. More specifically, when the composite pharmaceutical composition is used, the specification of Dongfeng Snail (40-50 heads) is ≥ 406 jin; further preferably, the specification of Dongfeng Snail (40-50 heads) is ≥ 420 jin. Further preferably, when the feed additive is used, the specification of Dongfeng Snail (40-50 heads) is ≥ 444 jin; further preferably, when the snail breeding feed is used, the specification of Dongfeng Snail (40-50 heads) is ≥ 458 jin; when the snail breeding feed is used, the specification of Dongfeng Snail (40-50 heads) is ≥ 473 jin.

[0177] It can be seen from Example 4, Example 19 and Example 20 that when the growth-promoting bacteria are a combination of Clostridium butyricum, Bifidobacterium, yeast and thermophilic Streptococcus, the growth performance of Babylonia snail can be better improved.

[0178] It can be seen from Example 4, Example 21, Example 22 and Example 23 that when the basic feed uses a combination of diatom mud, vitamin D, copper sulfate and amino acid chelated zinc, the growth performance of Babylonia snails can be better improved.

[0179] It can be seen from Example 4 and Comparative Examples 1-3 that the combination of Chinese herbal compound extracts and growth-promoting bacteria can synergistically improve the growth performance of Babylonia snails, and the specifications of Babylonia snails are significantly improved.

[0180] The foregoing examples are merely illustrative and are used to explain some features of the method of the present invention. The appended claims are intended to require the widest possible range that can be imagined, and the embodiments presented herein are demonstrated by the applicant's actual test results. Therefore, the applicant's intention is that the appended claims are not limited by the selection of examples that illustrate the features of the present invention. Some numerical ranges used in the claims also include sub-ranges therein, and changes in these ranges should also be interpreted as being covered by the appended claims where possible.

Claims

1. A compound pharmaceutical composition, characterized in that: It includes a Chinese herbal compound extract and growth-promoting bacteria in a mass ratio of 800-1200:20-85; The Chinese medicine composite extract is prepared by the following raw materials by weight: 10-12 parts of Shenqu, 8-12 parts of Eucommia ulmoides leaves, 6-8 parts of Astragalus, 6-8 parts of Yucca, 5-10 parts of Poria, 5-10 parts of Polygonum cuspidatum, 5-10 parts of Clove, 4-8 parts of Radish Seed, 4-8 parts of Uncaria rhynchophylla, 4-8 parts of Perilla frutescens leaves, 3-5 parts of Papaya, 3-6 parts of Leonurus japonicus, 3-6 parts of Taraxacum, 3-6 parts of Curcuma longa, 3-7 parts of Hawthorn, 3-5 parts of Isatis indigotica, 3-6 parts of Malt, and 3-6 parts of Mulberry leaves; The growth-promoting bacteria are selected from one or more of Clostridium butyricum, Bifidobacterium, yeast, and Streptococcus thermophilus.

2. The compound pharmaceutical composition according to claim 1, characterized in that: In terms of weight, the growth-promoting bacteria include: 1-2 parts of Clostridium butyricum, 1-2 parts of Bifidobacterium, 1-2 parts of yeast and 1-2 parts of Streptococcus thermophilus.

3. The method for preparing the composite pharmaceutical composition according to claim 1 or 2, characterized in that: The following steps are involved: S1. extracting the raw material of the Chinese medicine composite extract by alcohol extraction, and post-processing to obtain the Chinese medicine composite extract; S2. Evenly mix the traditional Chinese medicine compound extract and the growth-promoting bacteria, and ferment to obtain a compound pharmaceutical composition.

4. The method for preparing the composite pharmaceutical composition according to claim 3, characterized in that: Selected from any one of the following (a)-(e): (a) the mass ratio of the raw material to the alcohol solvent is 1:1.5-2; (b) the extraction temperature of the alcohol extraction method is 50-70°C; (c) the extraction is ultrasonic extraction; (d) the power of the ultrasonic extraction is 100-500W, and the time of the ultrasonic extraction is 20-40min; (e) The fermentation time is 30-50 days.

5. A feed additive, characterized in that: It comprises a basic base material, a seaweed extract, a traditional Chinese medicine compound extract and growth-promoting bacteria; the mass ratio of the basic base material, the seaweed extract, the traditional Chinese medicine compound extract and the growth-promoting bacteria is 500-650:300-400:800-1200:20-85; The Chinese medicine composite extract is prepared by the following raw materials by weight: 10-12 parts of Shenqu, 8-12 parts of Eucommia ulmoides leaves, 6-8 parts of Astragalus, 6-8 parts of Yucca, 5-10 parts of Poria, 5-10 parts of Polygonum cuspidatum, 5-10 parts of Clove, 4-8 parts of Radish Seed, 4-8 parts of Uncaria rhynchophylla, 4-8 parts of Perilla frutescens leaves, 3-5 parts of Papaya, 3-6 parts of Leonurus japonicus, 3-6 parts of Taraxacum, 3-6 parts of Curcuma longa, 3-7 parts of Hawthorn, 3-5 parts of Isatis indigotica, 3-6 parts of Malt, and 3-6 parts of Mulberry leaves; The growth-promoting bacteria are selected from one or more of Clostridium butyricum, Bifidobacterium, yeast, and Streptococcus thermophilus.

6. The feed additive according to claim 5, characterized in that: The seaweed extract is prepared from the following raw materials: Sargassum, Ulva, Laminaria japonica and Gracilaria lemaneiformis; Preferably, the mass ratio of the Sargassum, Ulva, Laminaria and Gracilaria is 3-5:1-2:2-3:2-3.

7. The feed additive according to claim 5 or 6, characterized in that: The basic base material includes one or more of scallop meat, grain worms, Artemia, squid paste, brewer's yeast, and coconut meat; Preferably, in terms of weight, the basic base material includes 10-15 parts of scallop meat, 8-14 parts of five-grain worms, 4-9 parts of artemia, 2-5 parts of cuttlefish paste, 1-2 parts of brewer's yeast, and 5-7 parts of coconut meat.

8. A feed for snail breeding, characterized in that: include: (a) 0.5-10.0% by weight of the composite pharmaceutical composition according to claim 1 or 2, or the feed additive according to any one of claims 5-7; (b) basic feed; Preferably, in terms of weight, the basic feed includes 4-6 parts of diatom mud, 1-3 parts of vitamin D, 0.5-1 parts of copper sulfate, 0.5-1 parts of amino acid chelated zinc, 20-40 parts of gluten powder, and 50-70 parts of fish meal.

9. The method for preparing the snail breeding feed according to claim 8, characterized in that: The composite pharmaceutical composition according to claim 1 or 2, or the feed additive according to any one of claims 5 to 7, is mixed with a basic feed, and subjected to puffing treatment and drying treatment to obtain a snail breeding feed.

10. Use of the composite pharmaceutical composition according to claim 1 or 2, or the feed additive according to any one of claims 5 to 7, or the snail breeding feed according to claim 8 in improving the growth performance of Babylonia snails.

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