Dietary fiber composition for reducing weight and targeting regulation of intestinal flora as well as preparation method and application of dietary fiber composition

Abstract

The invention provides a dietary fiber composition for reducing weight and regulating intestinal flora in a targeted manner as well as a preparation method and application of the dietary fiber composition. The composition is prepared from the following raw materials in parts by weight: 20 to 30 parts of inulin, 15 to 25 parts of germinated brown rice powder, 10 to 20 parts of pectin, 8 to 15 parts of guar gum, 12 to 20 parts of beta-glucan, 15 to 25 parts of konjac glucomannan, 10 to 20 parts of multi-element dietary fiber powder, 5 to 10 parts of compound probiotic powder, 3 to 8 parts of bitter gourd polypeptide, 3 to 8 parts of folium mori extract and 2 to 5 parts of protein hydrolysate. Multi-element dietary fiber powder, prebiotics and the like are combined, endogenous GLP-1 is stimulated, the characteristics that the protein nutrient is not easily absorbed by people, but can be utilized by intestinal flora to generate SCFA and stimulate GLP-1 secreted by L cells at the far end of the intestinal tract are fully exerted, the requirements of most people for weight losing and weight control can be met, the protein nutrient can serve as a source of DDP-IV inhibitory peptide, decomposition of the endogenous GLP-1 is slowed down, and the weight losing and weight control effects are achieved. A more lasting effect of endogenous GLP-1 is ensured; a hot extrusion embedding technology and an airflow cutting technology are adopted, so that more nutrient substances can be effectively reserved in intestinal tracts at the same time, and nutrients are provided for flora.

Description

Technical Field

[0001] The invention relates to the field of food and health food, and in particular to a dietary fiber composition for weight loss and targeted regulation of intestinal flora, and a preparation method and application thereof. Background Art

[0002] With the change of modern lifestyle and the adjustment of people's dietary structure, obesity problem is becoming more and more serious and has become a global public health challenge. Obesity not only affects personal appearance and quality of life, but is also closely related to the occurrence and development of a variety of chronic diseases, such as diabetes, cardiovascular disease, hypertension, etc. At the same time, intestinal flora plays a vital role in human health, and its imbalance is closely related to the occurrence of obesity and metabolic diseases.

[0003] At present, there are many kinds of weight loss products on the market, but they often have some shortcomings. Many weight loss drugs may cause side effects, such as cardiovascular problems, gastrointestinal discomfort, etc. And the simple dieting method of weight loss is difficult to stick to for a long time, and it is easy to lead to nutritional imbalance and slow metabolism. In addition, the effect of some weight loss foods is not significant, and they lack the effect of specifically regulating the intestinal flora to achieve weight loss.

[0004] Scientific research has shown that GLP-1 (glucagon-like peptide-1) is a glucagon-like peptide secreted by the human body, which can promote insulin gene transcription and insulin secretion, increase the sensitivity of receptors to insulin, and thus achieve the purpose of lowering blood sugar levels. At the same time, insulin can also promote fat synthesis and storage, reduce fat decomposition, and help maintain energy balance; GLP-1 can inhibit the secretion of glucagon by pancreatic α cells, reduce liver glycogenolysis and gluconeogenesis, and further reduce blood sugar levels. The reduction of glucagon also helps to reduce fat decomposition and energy release, which is beneficial for weight control.

[0005] The endogenous GLP-1 secreted by the body is easily degraded and cleared by dipeptidyl peptidase-4 (DPP-4). DPP-4 is widely present in human tissues and can quickly degrade GLP-1, making its half-life in the body very short, generally only 1-2 minutes, less than 25% of which has further catalytic activity in the liver, and only about 10-15% circulates in the body in its active form. Therefore, the prior art has developed DPP-4 inhibitors to prolong the action time of endogenous GLP-1, but most L cells are located in the distal epithelial layer of the intestine, and even with the use of DPP-4 inhibitors, it is difficult to stimulate L cells to secrete GLP-1, resulting in individuals with obesity and / or diabetes having difficulty in achieving weight loss. Summary of the invention

[0006] In view of this, the present invention proposes a dietary fiber composition for weight loss and targeted regulation of intestinal flora, as well as a preparation method and application thereof, to solve the above problems.

[0007] The technical solution of the present invention is achieved as follows: a dietary fiber composition for weight loss and targeted regulation of intestinal flora comprises the following raw materials in parts by weight: 20-30 parts of inulin, 15-25 parts of germinated brown rice flour, 10-20 parts of pectin, 8-15 parts of guar gum, 12-20 parts of beta-glucan, 15-25 parts of konjac glucomannan, 10-20 parts of multi-component dietary fiber powder, 5-10 parts of composite probiotic powder, 3-8 parts of bitter melon polypeptide, 3-8 parts of mulberry leaf extract, and 2-5 parts of protein hydrolyzate.

[0008] Furthermore, a dietary fiber composition for targeted regulation of intestinal flora for weight loss includes the following raw materials in parts by weight: 25 parts of inulin, 20 parts of germinated brown rice flour, 15 parts of pectin, 1 part of guar gum, 16 parts of β-glucan, 20 parts of konjac glucomannan, 15 parts of multi-dietary fiber powder, 8 parts of compound probiotic powder, 5 parts of bitter melon polypeptide, 5 parts of mulberry leaf extract, and 4 parts of protein hydrolyzate.

[0009] Furthermore, the multi-nutrient dietary fiber powder comprises wheat bran powder, oat bran powder, coix seed powder and buckwheat powder in a mass ratio of (4-6):(5-8):(4-9):(3-6).

[0010] Furthermore, the composite probiotic powder is animal bibacterium lactis subspecies CP-9, rhamnosus GM-020, and Lactobacillus reuteri GMNL-263, and the ratio of the above three strains is (14-25): (12-20): (8-15), and the number of viable bacteria is ≥ 1×10 8 cfu / mL.

[0011] Furthermore, the mulberry leaf extract is obtained by separating the protein precipitate in the fermentation liquid by centrifugation, filtration and the like after lactic acid bacteria fermentation.

[0012] Furthermore, the protein hydrolysate is one or a combination of whey protein hydrolysate, dark tea protein hydrolysate or pea protein hydrolysate.

[0013] Furthermore, a method for preparing a dietary fiber composition for weight loss and targeted regulation of intestinal flora comprises the following steps:

[0014] S1, mixing: inulin, germinated brown rice flour, pectin, guar gum, β-glucan, konjac glucomannan, multi-dietary fiber powder, and protein hydrolyzate are placed in a mixing device, and stirred at a speed of 30-60 rpm to fully mix the various ingredients, and the stirring time is 30-40 min;

[0015] S2. Embedding: The mixed material is fed into a hot extrusion device, the temperature of the hot extrusion device is set to 120-150°C, the pressure is controlled at 10-15MPa, the screw speed is 80-100rpm, and the hot extrusion time is 10-15min;

[0016] S3, remixing: adding the bitter melon polypeptide, mulberry leaf extract and composite probiotic powder to the material after hot extrusion treatment, stirring gently at 15-25 rpm for 20-30 min;

[0017] S4, airflow cutting: the material obtained in S3 is airflow cut into particles with a diameter of 0.1-0.5 mm, packaged, and dried to obtain a dietary fiber composition.

[0018] Furthermore, the airflow cutting of S4 controls the airflow velocity to be 50-80 m / s, the pressure to be 3-8 MPa, and the cutting angle to be 40-60°.

[0019] Furthermore, the composition is used in the preparation of health products with weight loss effects.

[0020] Furthermore, the composition is used in improving the intestinal microecological environment.

[0021] Furthermore, the composition is used in preventing and controlling metabolic diseases.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] The composition of the present invention adopts prebiotics such as inulin, germinated brown rice powder, pectin, guar gum, β-glucan, konjac glucomannan, etc. The multi-dietary fiber as a carbohydrate nutrient can be used by the intestinal flora to produce the endogenous short-chain fatty acid flora SCFA, which can stimulate the GLP-1 secreted by the distal intestinal L cells; bitter melon polypeptide, mulberry leaf extract and protein hydrolyzate as protein nutrients can be used as the source of DDP-IV inhibitory peptides, slow down the decomposition of endogenous GLP-1, and ensure a more lasting effect of endogenous GLP-1; the combination of carbohydrate nutrients and protein nutrients effectively improves the sustained effect of endogenous GLP-1. The composite probiotic powder with a specific ratio can be used to directionally proliferate the intestinal short-chain fatty acid flora SCFA, and improve blood sugar levels with multi-dietary fiber.

[0024] The preparation method of the present invention adopts a hot extrusion embedding process to ease the release of dietary fiber starch, form slowly digestible starch, and minimize the impact of possible quickly digestible starch; at the same time, more nutrients are retained in the intestine to provide nutrition for the flora. The powder particle size of the composition is refined by airflow cutting technology, which is conducive to the proliferation of beneficial bacteria. DETAILED DESCRIPTION

[0025] In order to better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention.

[0026] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0027] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial sources.

[0028] Example 1

[0029] A dietary fiber composition for weight loss and targeted regulation of intestinal flora: comprising the following raw materials in parts by weight: 20 parts of inulin, 15 parts of germinated brown rice flour, 10 parts of pectin, 8 parts of guar gum, 12 parts of β-glucan, 15 parts of konjac glucomannan, 10 parts of multi-component dietary fiber powder, 5 parts of composite probiotic powder, 3 parts of bitter melon polypeptide, 3 parts of mulberry leaf extract, and 2 parts of protein hydrolyzate;

[0030] The multi-element dietary fiber powder comprises wheat bran powder, oat bran powder, coix seed powder and buckwheat powder in a mass ratio of 4:5:4:3;

[0031] The composite probiotic powder contains animal bibacterium lactis subspecies CP-9, rhamnosus GM-020, and Lactobacillus reuteri GMNL-263, the ratio of the above three strains is 14:12:8, and the number of live bacteria is ≥1×10 8 cfu / mL;

[0032] The protein hydrolysate is whey protein hydrolysate.

[0033] Example 2

[0034] A dietary fiber composition for weight loss and targeted regulation of intestinal flora: comprising the following raw materials in parts by weight: 30 parts of inulin, 25 parts of germinated brown rice flour, 20 parts of pectin, 15 parts of guar gum, 20 parts of β-glucan, 25 parts of konjac glucomannan, 20 parts of multi-component dietary fiber powder, 10 parts of composite probiotic powder, 8 parts of bitter melon polypeptide, 8 parts of mulberry leaf extract, and 5 parts of protein hydrolyzate;

[0035] The multi-element dietary fiber powder comprises wheat bran powder, oat bran powder, coix seed powder and buckwheat powder in a mass ratio of 6:8:9:6;

[0036] The composite probiotic powder contains animal bibacterium lactis subspecies CP-9, rhamnosus GM-020, and Lactobacillus reuteri GMNL-263, the ratio of the above three strains is 25:20:15, and the number of live bacteria is ≥1×10 8 cfu / mL;

[0037] The protein hydrolysate is pea protein hydrolysate.

[0038] Example 3

[0039] A dietary fiber composition for weight loss and targeted regulation of intestinal flora: comprising the following raw materials in parts by weight: 25 parts of inulin, 20 parts of germinated brown rice flour, 15 parts of pectin, 1 part of guar gum, 16 parts of β-glucan, 20 parts of konjac glucomannan, 15 parts of multi-component dietary fiber powder, 8 parts of composite probiotic powder, 5 parts of bitter melon polypeptide, 5 parts of mulberry leaf extract, and 4 parts of protein hydrolyzate;

[0040] The multi-element dietary fiber powder comprises wheat bran powder, oat bran powder, coix seed powder and buckwheat powder in a mass ratio of 5:7:7:5;

[0041] The composite probiotic powder contains animal bibacterium lactis subspecies CP-9, rhamnosus GM-020, and Lactobacillus reuteri GMNL-263, the ratio of the above three strains is 20:16:12, and the number of live bacteria is ≥1×10 8 cfu / mL;

[0042] The protein hydrolysate is a whey protein hydrolysate, a dark tea protein hydrolysate or a pea protein hydrolysate in a mass ratio of 1:0.5:2;

[0043] The above examples 1-3 adopt the following preparation method:

[0044] S1, mixing: inulin, germinated brown rice flour, pectin, guar gum, β-glucan, konjac glucomannan, multi-dietary fiber powder, and protein hydrolyzate are placed in a mixing device, and stirred at a speed of 50 rpm to fully mix the various ingredients, and the stirring time is 35 min;

[0045] S2. Embedding: The mixed material is fed into a hot extrusion device, the temperature of the hot extrusion device is set to 130°C, the pressure is controlled at 12MPa, the screw speed is 90rpm, and the hot extrusion time is 13min;

[0046] S3, remixing: adding the bitter melon polypeptide, mulberry leaf extract and composite probiotic powder to the material after hot extrusion treatment, stirring gently at 20 rpm for 25 min;

[0047] S4, airflow cutting: the logistics obtained in S3 is airflow cut to a particle size of 0.3 mm, the airflow velocity is 60 m / s, the pressure is 5 MPa, the cutting angle is 50°, and the mixture is packaged and dried to obtain a dietary fiber composition.

[0048] Comparative Example 1

[0049] The difference between this comparative example and Example 3 is that the composite probiotic powder in the composition is replaced by lactic acid bacteria.

[0050] Comparative Example 2

[0051] The difference between this comparative example and Example 3 is that no protein hydrolysate is added to the composition.

[0052] Test Example 1

[0053] 1. Research subjects:

[0054] 180 volunteers aged 30-75;

[0055] Patients with type 2 diabetes: 6.5%<HbA1c<12%;

[0056] The weight was more than 75 kg, and the body mass index (BMI) was above 28%;

[0057] Exclusion criteria: severe complications, liver and kidney dysfunction, recent adjustment of hypoglycemic drugs, etc.

[0058] 2. Grouping and intervention:

[0059] The patients were randomly divided into 6 groups, 30 people in each group.

[0060] Example 1-3: On the basis of a regular diet, the above-prepared composition is additionally taken daily, with an intake of 30 g / day;

[0061] Comparative Example 1-2: On the basis of a regular diet, the above-prepared composition was additionally taken daily, with an intake of 30 g / day;

[0062] Control group: Follow the conventional diabetes diet management, without additional dietary fiber intake;

[0063] Consume for 2 months.

[0064] 3. Observation indicators:

[0065] Fasting blood glucose (FPG): Test once a month and take the average value.

[0066] Glycated hemoglobin (HbA1c): Test once before and after the experiment.

[0067] Total cholesterol (TC) and triglyceride (TG) were tested once before and after the experiment.

[0068] 4. Result data

[0069]

[0070] The above result data show that the high dietary fiber composition of the present invention has a certain effect in reducing fasting blood sugar, glycosylated hemoglobin, total cholesterol level, and triglyceride level, and there may be a dosage effect or a formula optimization effect.

[0071] Test Example 2

[0072] 1. Sample collection: Collect fresh stool samples from patients after the above interventions and ensure that the samples are delivered to the laboratory as soon as possible (within 5 minutes) after collection to reduce changes in microbial activity;

[0073] 2. Inoculation and culture: Inoculate the pretreated fecal sample into the culture medium and culture it at a constant temperature of 37°C.

[0074] 3. Identification and content determination: The cultured microorganisms are identified through morphological observation, physiological and biochemical characteristics testing, and molecular biology techniques. The organic acids in the samples are separated by HPLC, and the peak height or peak area of ​​butyric acid and acetic acid are measured by the detector to calculate their concentrations.

[0075] 4. Data results:

[0076] Butyrate-producing bacteria content Acetate-producing bacteria content Example 1 <![CDATA[1.0×10 9 ]]> <![CDATA[1.5×10 9 ]]> Example 2 <![CDATA[1.2×10 9 ]]> <![CDATA[1.8×10 9 ]]> Example 3 <![CDATA[1.5×10 9 ]]> <![CDATA[2.0×10 9 ]]> Comparative Example 1 <![CDATA[0.5×10 9 ]]> <![CDATA[0.8×10 9 ]]> Comparative Example 2 <![CDATA[0.3×10 9 ]]> <![CDATA[0.6×10 9 ]]> Control group <![CDATA[0.1×10 7 ]]> <![CDATA[0.3×10 8 ]]>

[0077] In Examples 1-3, the content of butyrate and acetate-producing bacteria in feces was high, indicating that the subjects had a healthier intestinal environment after using the composition of the present invention. High content of short-chain fatty acids such as butyrate and acetate has many benefits for intestinal health, including promoting the proliferation and repair of intestinal epithelial cells, enhancing intestinal barrier function, and reducing the risk of intestinal inflammation.

[0078] Test Example 3

[0079] The weight and body fat changes of the above patients were measured after 3 months;

[0080] 1. Main indicators

[0081] Weight: The subjects' weight was measured before and at the end of the experiment using a calibrated weight scale.

[0082] Body fat percentage: Body fat percentage is measured using methods such as bioelectrical impedance analysis (BIA) or dual-energy X-ray absorptiometry (DEXA).

[0083] 2. Secondary indicators

[0084] Waist circumference: Measure your waist circumference using a tape measure to assess changes in body fat distribution.

[0085] 3. Experimental results

[0086]

[0087] According to the above results, it is shown that the composition of the present invention can regulate the gastrointestinal tract and effectively achieve the effect of weight loss by controlling blood sugar.

[0088] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A dietary fiber composition for weight loss and targeted regulation of intestinal flora, characterized in that: The invention comprises the following raw materials in parts by weight: 20-30 parts of inulin, 15-25 parts of germinated brown rice flour, 10-20 parts of pectin, 8-15 parts of guar gum, 12-20 parts of beta-glucan, 15-25 parts of konjac glucomannan, 10-20 parts of multi-element dietary fiber powder, 5-10 parts of compound probiotic powder, 3-8 parts of bitter melon polypeptide, 3-8 parts of mulberry leaf extract and 2-5 parts of protein hydrolyzate.

2. A dietary fiber composition for weight loss and targeted regulation of intestinal flora according to claim 1, characterized in that: The invention comprises the following raw materials in parts by weight: 25 parts of inulin, 20 parts of germinated brown rice flour, 15 parts of pectin, 1 part of guar gum, 16 parts of beta-glucan, 20 parts of konjac glucomannan, 15 parts of multi-element dietary fiber powder, 8 parts of compound probiotic powder, 5 parts of bitter melon polypeptide, 5 parts of mulberry leaf extract and 4 parts of protein hydrolyzate.

3. A dietary fiber composition for weight loss and targeted regulation of intestinal flora according to claim 1, characterized in that: The multi-element dietary fiber powder comprises wheat bran powder, oat bran powder, coix seed powder and buckwheat powder in a mass ratio of (4-6):(5-8):(4-9):(3-6).

4. A dietary fiber composition for weight loss and targeted regulation of intestinal flora according to claim 1, characterized in that: The composite probiotic powder comprises animal bibacterium lactis subspecies CP-9, rhamnosus GM-020, and Lactobacillus reuteri GMNL-263, wherein the ratio of the three strains is (14-25):(12-20):(8-15), and the number of viable bacteria is ≥1×10 8 cfu / mL.

5. The dietary fiber composition for weight loss and targeted regulation of intestinal flora according to claim 1, characterized in that: The protein hydrolysate is one or a combination of whey protein hydrolysate, dark tea protein hydrolysate or pea protein hydrolysate.

6. The method for preparing a dietary fiber composition for weight loss and targeted regulation of intestinal flora according to claim 1, characterized in that: The following steps are involved: S1, mixing: inulin, germinated brown rice flour, pectin, guar gum, β-glucan, konjac glucomannan, multi-dietary fiber powder, and protein hydrolyzate are placed in a mixing device, and stirred at a speed of 30-60 rpm to fully mix the various ingredients, and the stirring time is 30-40 min; S2. Embedding: The mixed material is fed into a hot extrusion device, the temperature of the hot extrusion device is set to 120-150°C, the pressure is controlled at 10-15MPa, the screw speed is 80-100rpm, and the hot extrusion time is 10-15min; S3, remixing: adding the bitter melon polypeptide, mulberry leaf extract and composite probiotic powder to the material after hot extrusion treatment, stirring gently at 15-25 rpm for 20-30 min; S4, airflow cutting: the material obtained in S3 is airflow cut into particles with a diameter of 0.1-0.5 mm, packaged, and dried to obtain a dietary fiber composition.

7. A dietary fiber composition for weight loss and targeted regulation of intestinal flora according to claim 6, characterized in that: The airflow cutting of S4 controls the airflow velocity to be 50-80 m / s, the pressure to be 3-8 MPa, and the cutting angle to be 40-60°.

8. Use of the composition according to any one of claims 1 to 5 in the preparation of a health product with a weight loss effect.

9. Use of the composition according to any one of claims 1 to 5 in improving intestinal microecological environment.

10. Use of the composition according to any one of claims 1 to 5 in preventing and controlling metabolic diseases.

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