Skin-repairing anti-allergy skin care product with nitric oxide precursor fused with polypeptide

Abstract

The invention discloses a skin-repairing anti-allergy skin care product with nitric oxide precursor fused with polypeptide, and a method comprises the following steps: firstly, cleaning an operation area, preparing a stirring rod, a mixing container, a heating plate, raw materials and weighing equipment, then disinfecting tools, weighing the raw materials according to a proportion, and preparing the skin-repairing anti-allergy skin care product with the nitric oxide precursor fused with the polypeptide. Respectively weighing a proper amount of water-phase components including water and glycerol and oil-phase components including oily active components and an emulsifier in different containers; starting to emulsify, heating the oil phase and the water phase above a heating plate to the same temperature, and slowly adding the water phase into the oil phase; the nitric oxide precursor fusion polypeptide is added into the components, so that skin inflammation and sensitive reaction can be relieved, skin soothing and repairing are promoted, meanwhile, in the preparation method, the pH value of a sample is adjusted firstly, then the amount of the adjusting agent is calculated according to the amount of a finished product, the pH value of the skin care product can be adjusted more accurately, and the skin care effect is better. The preparation of skin care products is convenient.

Description

Technical Field

[0001] The present invention relates to the technical field of skin care products, and in particular to a skin repairing and anti-allergic skin care product containing a nitric oxide precursor fusion polypeptide. Background Art

[0002] Skin care products are products specially designed to maintain, improve and protect skin health. They contain various active ingredients and excipients to meet different skin needs. At the same time, the effects of skin care products vary depending on the product type and ingredients.

[0003] However, the main purpose of existing skin care products is to improve the moisture level of the skin. When the skin has problems such as allergies and inflammation, skin care products that only replenish water cannot meet the skin's needs. Therefore, a skin repair and anti-allergic skin care product fused with nitric oxide precursor polypeptide is proposed.

[0004] Currently, no effective solution has been proposed for the problems in the related technologies. Summary of the invention

[0005] In view of the problems in the related art, the present invention proposes a skin repairing and anti-allergic skin care product of nitric oxide precursor fusion polypeptide to overcome the above-mentioned technical problems existing in the existing related technology.

[0006] To this end, the specific technical solution adopted by the present invention is as follows:

[0007] A skin repair and anti-allergic skin care product containing a nitric oxide precursor fusion polypeptide, comprising the following ingredients:

[0008] Nitric oxide (NO) precursor, polypeptide, moisturizing agent, antioxidant, carrier, auxiliary component, the proportion of these components is 0.1% of nitric oxide (NO) precursor, 7.28% of polypeptide, 9.88% of moisturizing agent, 3.65% of antioxidant, 78.59% of carrier and 0.5% of auxiliary component;

[0009] The nitric oxide (NO) precursor is nitrite, the polypeptide includes sedative peptide and anti-inflammatory peptide, the moisturizer includes glycerin and hyaluronic acid, the antioxidant includes vitamin C and vitamin E, the carrier is an emulsifier, a base oil, water or a water-based component, a gel, and the auxiliary component is a preservative.

[0010] In the formula of skin care products, a certain proportion of preservatives is usually included to prevent the product from being contaminated by microorganisms such as bacteria and mold.

[0011] A method for preparing a skin repairing and anti-allergic skin care product fused with a nitric oxide precursor polypeptide comprises the following steps:

[0012] S1. First, clean the operation area, prepare a stirring rod, a mixing container, a heating plate, raw materials and a weighing device, sterilize the tools, weigh the raw materials according to the proportion, and weigh appropriate amounts of water phase components including water and glycerin and oil phase components including oily active ingredients and emulsifiers in different containers;

[0013] S2, start emulsification, place the oil phase and the water phase on a heating plate and heat them to the same temperature, then slowly add the water phase to the oil phase, and stir evenly using an emulsifier or a stirring rod until an emulsion is formed;

[0014] S3, slowly adding the active ingredient nitric oxide precursor and polypeptide solution into the emulsion;

[0015] S4, adding antioxidants and preservatives, and adjusting the pH value according to the formulation requirements;

[0016] S5. After the finished product is fully mixed, it is filled into a clean container, sealed and stored, and the final product is subjected to microbiological and stability tests to ensure that the product meets safety and quality standards.

[0017] As a preferred embodiment, the operation area is first cleaned, a stirring rod, a mixing container, a heating plate, raw materials and a weighing device are prepared, the tools are sterilized, and the raw materials are weighed according to the proportion, which includes the following steps:

[0018] S11. Remove irrelevant items from the work area, keep the work surface clean, and then wipe the work surface with a detergent to ensure that there is no dust, dirt or residue on the surface;

[0019] S12, check the status of the stirring rod and the mixing container to ensure that they are clean and firm, then prepare the heating plate and the weighing equipment, confirm that the heating plate is working properly, check whether the heating temperature control function is accurate, and then calibrate the electronic scale;

[0020] S13. First, use high-temperature steam to sterilize the stirring rod and the mixing container, then air-dry the stirring rod and the mixing container after sterilization, and ensure that the stirring rod and the mixing container are in a dry state during use, and then use an appropriate disinfectant to wipe the surface of the heating plate and the weighing device to ensure that there are no impurities or contaminants on the surface;

[0021] Steam can quickly transfer heat and moisture, effectively killing most microorganisms, including bacteria, fungi and viruses. At the same time, steam sterilization does not use any chemicals, avoiding the potential impact of chemical residues on product quality, and is suitable for the manufacture of skin care products.

[0022] S14. First, select an appropriate mixing container for weighing the water phase components, and then select another clean mixing container for weighing the oil phase components. When weighing, accurately weigh the required amount according to the ratio of the water phase and oil phase components in the formula.

[0023] As a preferred embodiment, the emulsification is started, the oil phase and the water phase are placed on a heating plate and heated to the same temperature, and then the water phase is slowly added to the oil phase, and stirred evenly with an emulsifier or a stirring rod until an emulsion is formed, which comprises the following steps:

[0024] S21, placing the weighed oil phase and water phase on a heating plate respectively, and heating them to the same temperature, and then slowly adding the hot water phase to the hot oil phase;

[0025] When adding the water phase to the oil phase, you need to operate slowly to ensure that the water phase is added evenly to the oil phase.

[0026] S22. Use a stirring rod to evenly stir the mixture. During the stirring process, observe the appearance and texture of the mixture to ensure that the emulsification process is fully completed and the mixture eventually becomes a uniform emulsion.

[0027] S23, placing the emulsified emulsion in the working area for natural cooling.

[0028] As a preferred embodiment, the step of slowly adding the active ingredient nitric oxide precursor and polypeptide solution into the emulsion comprises the following steps:

[0029] S31. Use a burette to slowly and evenly add the active ingredient solution to the emulsion, and then use a stirring rod to slowly stir to ensure that the active ingredient is fully dispersed and fully combined with other ingredients in the emulsion.

[0030] Adding the solution slowly helps to reduce the formation of bubbles, thereby reducing the impact of bubbles on the quality of the emulsion.

[0031] As a preferred embodiment, the adding of antioxidants and preservatives and adjusting the pH value according to the formulation requirements comprises the following steps:

[0032] S41, adding antioxidants and preservatives into the emulsion, and stirring and mixing them thoroughly with a stirring rod;

[0033] S42, sampling the finished emulsion, and using a pH meter to test the pH value, ensuring that the pH value range is 5.0 to 6.0, when the pH value is lower than 5.0, adjusting the pH value of the emulsion by titrating sodium hydroxide solution, when the pH value is higher than 6.0, adjusting the pH value of the emulsion by titrating citric acid solution, when the pH value of the emulsion reaches the target range, stop adding the adjusting agent, and continue stirring for a few minutes to ensure uniform mixing, and finally record the pH value before and after adjustment, as well as the amount and time of adding the adjusting agent;

[0034] When the pH value of the finished emulsion is within the specified range, proceed directly to the next step.

[0035] As a preferred embodiment, the finished emulsion is sampled and the pH value is tested using a pH meter to ensure that the pH value ranges from 5.0 to 6.0. When the pH value is lower than 5.0, the pH value of the emulsion is adjusted by titrating sodium hydroxide solution. When the pH value is higher than 6.0, the pH value of the emulsion is adjusted by titrating citric acid solution. When the pH value of the emulsion reaches the target range, the addition of the adjusting agent is stopped, and stirring is continued for a few minutes to ensure uniform mixing. The steps include:

[0036] S421, sampling a portion of the sample from the finished emulsion, and recording the sample weight, using a pH meter to measure the current pH value of the sampled emulsion, and recording the initial value;

[0037] S422, when the pH value of the emulsion is lower than 5.0, a sodium hydroxide solution is selected as an adjusting agent, and the sodium hydroxide solution is added to the emulsion using a burette, and the titration amount of the sodium hydroxide solution is recorded during titration. After each titration of a certain amount of sodium hydroxide solution, the emulsion is continuously stirred for 5 minutes, and the pH value of the sample is detected using a pH meter. When the pH value of the emulsion reaches the target range, the addition of the adjusting agent is stopped;

[0038] S423, when the pH value of the emulsion is higher than 6.0, select citric acid solution as an adjusting agent, and use a burette to add the citric acid solution to the emulsion, record the titration amount of the citric acid solution during titration, and continue to stir the emulsion for 5 minutes after each titration of a certain amount of citric acid solution, and use a pH meter to detect the pH value of the sample. When the pH value of the emulsion reaches the target range, stop adding the adjusting agent;

[0039] S424. Calculate the amount of adjuster that should be added to the finished emulsion based on the recorded sample weight and adjustment dosage, then add the adjuster to the finished emulsion and mix thoroughly. After the mixing is completed, take a sample again to test the pH value to ensure that the pH value range is 5.0 to 6.0.

[0040] pH meters can provide very precise pH values ​​and are therefore suitable for cosmetic formulation adjustments.

[0041] As a preferred embodiment, the finished product is fully mixed, filled into a clean container, sealed and stored, and the final product is subjected to microbiological and stability tests to ensure that the product meets safety and quality standards, including the following steps:

[0042] S51. Fill the finished emulsion with adjusted pH value into clean and sterilized containers to ensure that there is no bacterial contamination on the surface of the container. After filling, seal the container in time to prevent air and external contaminants from entering;

[0043] S52. Take samples from the finished product again for microbiological and stability tests to assess the quality and shelf life of the product;

[0044] S53. Record the results of microbiological and stability testing to ensure that products meet safety and quality standards.

[0045] Final processing and testing of the finished emulsion is to ensure product quality, so the testing step is one of the key steps.

[0046] The beneficial effects of the present invention are:

[0047] 1. The present invention can achieve the benefits of promoting blood circulation and skin oxygenation, enhancing the skin's antioxidant capacity, promoting collagen production, soothing and repairing the skin by adding a nitric oxide precursor fusion polypeptide to the ingredients. Adding an appropriate amount of nitric oxide can help reduce skin inflammation and allergic reactions, thereby promoting skin soothing and repair, which is particularly effective for sensitive skin or damaged skin. In addition, nitric oxide can also improve the skin's luster and make the skin more elastic.

[0048] 2. The present invention controls the pH value of skin care products within the range of 5.0-6.0, so that the skin care products can better match the natural acid-base balance of the skin, thereby helping to maintain the health of the skin. At the same time, since pH values ​​above or below the normal range may increase the irritation of skin care products to the skin and even cause allergic reactions, controlling the pH between 5.0 and 6.0 can reduce these risks and maintain skin stability.

[0049] 3. When conducting pH value detection, the present invention first adjusts the pH value of the sample, and records the amount of the adjusting agent used when adjusting the pH value of the sample, and then calculates the amount of adjusting agent that should be added to the finished product based on the recorded amount of adjusting agent, so that the pH value of the finished emulsion can be adjusted more accurately, avoiding direct adjustment of the finished emulsion resulting in the pH value adjustment exceeding the normal range, and reducing the probability of failure in the production of skin care products due to failure of pH value adjustment. BRIEF DESCRIPTION OF THE DRAWINGS

[0050] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0051] Figure 1 The present invention is a flow chart of the preparation of a skin repairing and anti-allergic skin care product comprising a nitric oxide precursor fusion polypeptide according to an embodiment of the present invention. DETAILED DESCRIPTION

[0052] To further illustrate each embodiment, the present invention provides drawings, which are part of the disclosure of the present invention and are mainly used to illustrate the embodiments and can be used in conjunction with the relevant descriptions in the specification to explain the operating principles of the embodiments. With reference to these contents, ordinary technicians in the field should be able to understand other possible implementations and advantages of the present invention. The components in the figures are not drawn to scale, and similar component symbols are generally used to represent similar components.

[0053] According to an embodiment of the present invention, a skin repairing and anti-allergic skin care product containing a nitric oxide precursor fusion polypeptide is provided.

[0054] The present invention is further described with reference to the accompanying drawings and specific embodiments. Figure 1 As shown, a skin repair and anti-allergic skin care product of a nitric oxide precursor fusion polypeptide according to an embodiment of the present invention comprises the following ingredients:

[0055] Nitric oxide (NO) precursor, polypeptide, moisturizing agent, antioxidant, carrier, auxiliary component, the proportion of these components is 0.1% of nitric oxide (NO) precursor, 7.28% of polypeptide, 9.88% of moisturizing agent, 3.65% of antioxidant, 78.59% of carrier and 0.5% of auxiliary component;

[0056] The nitric oxide (NO) precursor is nitrite, the polypeptide includes sedative peptide and anti-inflammatory peptide, the moisturizer includes glycerin and hyaluronic acid, the antioxidant includes vitamin C and vitamin E, the carrier is an emulsifier, a base oil, water or a water-based component, a gel, and the auxiliary component is a preservative.

[0057] It should be noted that nitrite can release nitric oxide in aqueous solution. Its chemical properties and stability make it suitable for use in skin care products. Nitrite can bring skin care effects at the correct concentration, such as promoting blood circulation and enhancing skin radiance.

[0058] A method for preparing a skin repairing and anti-allergic skin care product fused with a nitric oxide precursor polypeptide comprises the following steps:

[0059] S1. First, clean the operation area, prepare a stirring rod, a mixing container, a heating plate, raw materials and a weighing device, sterilize the tools, weigh the raw materials according to the proportion, and weigh appropriate amounts of water phase components including water and glycerin and oil phase components including oily active ingredients and emulsifiers in different containers;

[0060] Furthermore, the process of first cleaning the operating area, preparing a stirring rod, a mixing container, a heating plate, raw materials and a weighing device, and then sterilizing the tools, and weighing the raw materials according to the proportion includes the following steps:

[0061] S11. Remove irrelevant items from the work area, keep the work surface clean, and then wipe the work surface with a detergent to ensure that there is no dust, dirt or residue on the surface;

[0062] S12, check the status of the stirring rod and the mixing container to ensure that they are clean and firm, then prepare the heating plate and the weighing equipment, confirm that the heating plate is working properly, check whether the heating temperature control function is accurate, and then calibrate the electronic scale;

[0063] S13. First, use high-temperature steam to sterilize the stirring rod and the mixing container, then air-dry the stirring rod and the mixing container after sterilization, and ensure that the stirring rod and the mixing container are in a dry state during use, and then use an appropriate disinfectant to wipe the surface of the heating plate and the weighing device to ensure that there are no impurities or contaminants on the surface;

[0064] S14. First, select an appropriate mixing container for weighing the water phase components, and then select another clean mixing container for weighing the oil phase components. When weighing, accurately weigh the required amount according to the ratio of the water phase and oil phase components in the formula.

[0065] It should be noted that when performing disinfection operations, it is necessary to record the time, method and disinfectant information used for cleaning and disinfection in order to conduct strict production control;

[0066] When weighing raw materials, it is necessary to record information such as the batch number, weighed quantity, production date, etc. for subsequent traceability and quality control.

[0067] S2, start emulsification, place the oil phase and the water phase on a heating plate and heat them to the same temperature, then slowly add the water phase to the oil phase, and stir evenly using an emulsifier or a stirring rod until an emulsion is formed;

[0068] Furthermore, the emulsification is started by placing the oil phase and the water phase on a heating plate and heating them to the same temperature, then slowly adding the water phase to the oil phase, and stirring evenly with an emulsifier or a stirring rod until an emulsion is formed, which comprises the following steps:

[0069] S21, placing the weighed oil phase and water phase on a heating plate respectively, and heating them to the same temperature, and then slowly adding the hot water phase to the hot oil phase;

[0070] It should be noted that if the temperature difference between the oil phase and the water phase is too large, uneven emulsification or inadequate dissolution of the emulsifier may occur during the mixing process, affecting the quality of the final product. Therefore, heating to the same temperature can ensure that the oil phase and the water phase have similar fluidity and viscosity, which is conducive to their more even mixing.

[0071] S22. Use a stirring rod to evenly stir the mixture. During the stirring process, observe the appearance and texture of the mixture to ensure that the emulsification process is fully completed and the mixture eventually becomes a uniform emulsion.

[0072] S23, placing the emulsified emulsion in the working area for natural cooling

[0073] S3, slowly adding the active ingredient nitric oxide precursor and polypeptide solution into the emulsion;

[0074] Further, S31, using a burette, slowly and evenly add the active ingredient solution to the emulsion, and then slowly stir using a stirring rod to ensure that the active ingredient is fully dispersed and fully combined with other ingredients in the emulsion.

[0075] It should be noted that the use of a burette has the following benefits, including: providing accurate flow control, avoiding local excessive concentration, promoting uniform mixing, ensuring accurate dosage, reducing bubble generation, and also facilitating observation and recording of the process.

[0076] S4, adding antioxidants and preservatives, and adjusting the pH value according to the formulation requirements;

[0077] Furthermore, the adding of antioxidants and preservatives and adjusting the pH value according to the formulation requirements comprises the following steps:

[0078] S41, adding antioxidants and preservatives into the emulsion, and stirring and mixing them thoroughly with a stirring rod;

[0079] S42, sampling the finished emulsion, and using a pH meter to test the pH value, ensuring that the pH value range is 5.0 to 6.0, when the pH value is lower than 5.0, adjusting the pH value of the emulsion by titrating sodium hydroxide solution, when the pH value is higher than 6.0, adjusting the pH value of the emulsion by titrating citric acid solution, when the pH value of the emulsion reaches the target range, stop adding the adjusting agent, and continue stirring for a few minutes to ensure uniform mixing, and finally record the pH value before and after adjustment, as well as the amount and time of adding the adjusting agent;

[0080] When the pH value of the finished emulsion is within the specified range, proceed directly to the next step.

[0081] Further, the finished emulsion is sampled and the pH value is tested using a pH meter to ensure that the pH value ranges from 5.0 to 6.0. When the pH value is lower than 5.0, the pH value of the emulsion is adjusted by titrating sodium hydroxide solution. When the pH value is higher than 6.0, the pH value of the emulsion is adjusted by titrating citric acid solution. When the pH value of the emulsion reaches the target range, the addition of the adjusting agent is stopped, and stirring is continued for a few minutes to ensure uniform mixing, which includes the following steps:

[0082] S421, sampling a portion of the sample from the finished emulsion, and recording the sample weight, using a pH meter to measure the current pH value of the sampled emulsion, and recording the initial value;

[0083] S422, when the pH value of the emulsion is lower than 5.0, a sodium hydroxide solution is selected as an adjusting agent, and the sodium hydroxide solution is added to the emulsion using a burette, and the titration amount of the sodium hydroxide solution is recorded during titration. After each titration of a certain amount of sodium hydroxide solution, the emulsion is continuously stirred for 5 minutes, and the pH value of the sample is detected using a pH meter. When the pH value of the emulsion reaches the target range, the addition of the adjusting agent is stopped;

[0084] S423, when the pH value of the emulsion is higher than 6.0, select citric acid solution as an adjusting agent, and use a burette to add the citric acid solution to the emulsion, record the titration amount of the citric acid solution during titration, and continue to stir the emulsion for 5 minutes after each titration of a certain amount of citric acid solution, and use a pH meter to detect the pH value of the sample. When the pH value of the emulsion reaches the target range, stop adding the adjusting agent;

[0085] S424. Calculate the amount of adjuster that should be added to the finished emulsion based on the recorded sample weight and adjustment dosage, then add the adjuster to the finished emulsion and mix thoroughly. After the mixing is completed, take a sample again to test the pH value to ensure that the pH value range is 5.0 to 6.0.

[0086] It should be noted that by first testing and adjusting the pH value of the sample, and then calculating the amount of the adjuster that should be added to the finished emulsion based on the total dose of the sample titrated, it can be ensured that the pH value of the finished emulsion can be accurately adjusted to avoid excessive adjuster.

[0087] S5. After the finished product is fully mixed, it is filled into a clean container, sealed and stored, and the final product is tested for microorganisms and stability to ensure that the product meets safety and quality standards

[0088] Furthermore, after the finished product is fully mixed, it is filled into a clean container, sealed and stored, and the final product is subjected to microbiological testing and stability testing to ensure that the product meets safety and quality standards, which includes the following steps:

[0089] S51. Fill the finished emulsion with adjusted pH value into clean and sterilized containers to ensure that there is no bacterial contamination on the surface of the container. After filling, seal the container in time to prevent air and external contaminants from entering;

[0090] S52. Take samples from the finished product again for microbiological and stability tests to assess the quality and shelf life of the product;

[0091] S53. Record the results of microbiological and stability testing to ensure that products meet safety and quality standards.

[0092] It should be noted that microbiological testing is performed to ensure that the product is free of microbial contamination, thereby ensuring that the product does not pose a health risk to users during use;

[0093] Stability testing is performed to evaluate the performance of a product under different conditions to ensure that the product quality remains stable during its shelf life.

[0094] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A skin repair and anti-allergic skin care product containing a nitric oxide precursor fusion polypeptide, characterized in that: This skin care product includes the following ingredients: Nitric oxide (NO) precursor, polypeptide, moisturizing agent, antioxidant, carrier, auxiliary component, the proportion of these components is 0.1% of nitric oxide (NO) precursor, 7.28% of polypeptide, 9.88% of moisturizing agent, 3.65% of antioxidant, 78.59% of carrier and 0.5% of auxiliary component; The nitric oxide (NO) precursor is nitrite, the polypeptide includes sedative peptide and anti-inflammatory peptide, the moisturizer includes glycerin and hyaluronic acid, the antioxidant includes vitamin C and vitamin E, the carrier is an emulsifier, a base oil, water or a water-based component, a gel, and the auxiliary component is a preservative.

2. A method for preparing a skin repairing and anti-allergic skin care product containing a nitric oxide precursor fusion polypeptide, characterized in that: The following steps are involved: S1. First, clean the operation area, prepare a stirring rod, a mixing container, a heating plate, raw materials and a weighing device, sterilize the tools, weigh the raw materials according to the proportion, and weigh appropriate amounts of water phase components including water and glycerin and oil phase components including oily active ingredients and emulsifiers in different containers; S2, start emulsification, place the oil phase and the water phase on a heating plate and heat them to the same temperature, then slowly add the water phase to the oil phase, and stir evenly using an emulsifier or a stirring rod until an emulsion is formed; S3, slowly adding the active ingredient nitric oxide precursor and polypeptide solution into the emulsion; S4, adding antioxidants and preservatives, and adjusting the pH value according to the formulation requirements; S5. After the finished product is fully mixed, it is filled into a clean container, sealed and stored, and the final product is subjected to microbiological and stability tests to ensure that the product meets safety and quality standards.

3. The method for preparing a skin repairing and anti-allergic skin care product of a nitric oxide precursor fusion polypeptide according to claim 1, characterized in that: The process of first cleaning the operating area, preparing a stirring rod, a mixing container, a heating plate, raw materials and a weighing device, and then sterilizing the tools, and weighing the raw materials according to the proportion includes the following steps: S11. Remove irrelevant items from the work area, keep the work surface clean, and then wipe the work surface with a detergent to ensure that there is no dust, dirt or residue on the surface; S12, check the status of the stirring rod and the mixing container to ensure that they are clean and firm, then prepare the heating plate and the weighing equipment, confirm that the heating plate is working properly, check whether the heating temperature control function is accurate, and then calibrate the electronic scale; S13. First, use high-temperature steam to sterilize the stirring rod and the mixing container, then air-dry the stirring rod and the mixing container after sterilization, and ensure that the stirring rod and the mixing container are in a dry state during use, and then use an appropriate disinfectant to wipe the surface of the heating plate and the weighing device to ensure that there are no impurities or contaminants on the surface; S14. First, select an appropriate mixing container for weighing the water phase components, and then select another clean mixing container for weighing the oil phase components. When weighing, accurately weigh the required amount according to the ratio of the water phase and oil phase components in the formula.

4. The method for preparing a skin repairing and anti-allergic skin care product of a nitric oxide precursor fusion polypeptide according to claim 1, characterized in that: The process of starting emulsification, placing the oil phase and the water phase on a heating plate and heating them to the same temperature, then slowly adding the water phase to the oil phase, and stirring evenly with an emulsifier or a stirring rod until an emulsion is formed comprises the following steps: S21, placing the weighed oil phase and water phase on a heating plate respectively, and heating them to the same temperature, and then slowly adding the hot water phase to the hot oil phase; S22. Use a stirring rod to evenly stir the mixture. During the stirring process, observe the appearance and texture of the mixture to ensure that the emulsification process is fully completed and the mixture eventually becomes a uniform emulsion. S23, placing the emulsified emulsion in the working area for natural cooling.

5. The method for preparing a skin repairing and anti-allergic skin care product of a nitric oxide precursor fusion polypeptide according to claim 1, characterized in that: The method of slowly adding the active ingredient nitric oxide precursor and polypeptide solution into the emulsion comprises the following steps: S31. Use a burette to slowly and evenly add the active ingredient solution to the emulsion, and then use a stirring rod to slowly stir to ensure that the active ingredient is fully dispersed and fully combined with other ingredients in the emulsion.

6. The method for preparing a skin repairing and anti-allergic skin care product of a nitric oxide precursor fusion polypeptide according to claim 1, characterized in that: The adding of antioxidants and preservatives and adjusting the pH value according to the formulation requirements comprises the following steps: S41, adding antioxidants and preservatives into the emulsion, and stirring and mixing them thoroughly with a stirring rod; S42, sampling the finished emulsion, and using a pH meter to test the pH value, ensuring that the pH value range is 5.0 to 6.0, when the pH value is lower than 5.0, adjusting the pH value of the emulsion by titrating sodium hydroxide solution, when the pH value is higher than 6.0, adjusting the pH value of the emulsion by titrating citric acid solution, when the pH value of the emulsion reaches the target range, stop adding the adjusting agent, and continue stirring for a few minutes to ensure uniform mixing, and finally record the pH value before and after adjustment, as well as the amount and time of adding the adjusting agent; When the pH value of the finished emulsion is within the specified range, proceed directly to the next step.

7. The method for preparing a skin repairing and anti-allergic skin care product of a nitric oxide precursor fusion polypeptide according to claim 1, characterized in that: The finished emulsion is sampled and tested for pH value using a pH meter to ensure that the pH value ranges from 5.0 to 6.

0. When the pH value is lower than 5.0, the pH value of the emulsion is adjusted by titrating sodium hydroxide solution. When the pH value is higher than 6.0, the pH value of the emulsion is adjusted by titrating citric acid solution. When the pH value of the emulsion reaches the target range, the addition of the adjusting agent is stopped, and stirring is continued for several minutes to ensure uniform mixing. The steps include: S421, sampling a portion of the sample from the finished emulsion, and recording the sample weight, using a pH meter to measure the current pH value of the sampled emulsion, and recording the initial value; S422, when the pH value of the emulsion is lower than 5.0, a sodium hydroxide solution is selected as an adjusting agent, and the sodium hydroxide solution is added to the emulsion using a burette, and the titration amount of the sodium hydroxide solution is recorded during titration. After each titration of a certain amount of sodium hydroxide solution, the emulsion is continuously stirred for 5 minutes, and the pH value of the sample is detected using a pH meter. When the pH value of the emulsion reaches the target range, the addition of the adjusting agent is stopped; S423, when the pH value of the emulsion is higher than 6.0, select citric acid solution as an adjusting agent, and use a burette to add the citric acid solution to the emulsion, record the titration amount of the citric acid solution during titration, and continue to stir the emulsion for 5 minutes after each titration of a certain amount of citric acid solution, and use a pH meter to detect the pH value of the sample. When the pH value of the emulsion reaches the target range, stop adding the adjusting agent; S424. Calculate the amount of adjuster that should be added to the finished emulsion based on the recorded sample weight and adjustment dosage, then add the adjuster to the finished emulsion and mix thoroughly. After the mixing is completed, take a sample again to test the pH value to ensure that the pH value range is 5.0 to 6.

0.

8. The method for preparing a skin repairing and anti-allergic skin care product of a nitric oxide precursor fusion polypeptide according to claim 1, characterized in that: After the finished product is fully mixed, it is filled into a clean container, sealed and stored, and the final product is subjected to microbiological and stability tests to ensure that the product meets safety and quality standards, including the following steps: S51. Fill the finished emulsion with adjusted pH value into clean and sterilized containers to ensure that there is no bacterial contamination on the surface of the container. After filling, seal the container in time to prevent air and external contaminants from entering; S52. Take samples from the finished product again for microbiological and stability tests to assess the quality and shelf life of the product; S53. Record the results of microbiological and stability testing to ensure that products meet safety and quality standards.

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