Polygonatum sibiricum powder and production method thereof

Abstract

The invention discloses rhizoma polygonati powder and a production method thereof, and belongs to the field of traditional Chinese medicines. The method comprises the following specific steps: taking fresh rhizoma polygonati tubers, removing capillary roots and impurities, washing with clear water, and airing; beating into slurry by using a colloid mill, and adding a proper amount of water in the process to obtain slurry; heating the obtained slurry, sequentially carrying out enzymolysis through cellulase, pectinase, alpha-amylase and saccharifying enzyme, adjusting the pH value of enzymatic hydrolysate, carrying out enzyme inactivation treatment, and then carrying out drying treatment to obtain the rhizoma polygonati powder. The production method is short in period, simple in process and suitable for large-scale production, and the prepared rhizoma polygonati powder is slightly sweet in taste, free of numbness and high in polysaccharide content.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicine, and more particularly to polygonatum sibiricum powder and a production method thereof. Background Art

[0002] Polygonatum sibiricum, the dried rhizome of Polygonatum yunnanensis, Polygonatum sibiricum or Polygonatum sibiricum of the Liliaceae family, has important medicinal value. Studies have shown that Polygonatum sibiricum has antioxidant and anti-aging functions. It can enhance and regulate the body's immune function, activate endogenous substances that defend against free radical damage, and inhibit oxygen free radicals in aging animals. Polygonatum sibiricum also has the function of regulating immunity. Polygonatum sibiricum total saponins can significantly increase the thymus and spleen indexes and the amount of serum immunoglobulins and interleukin-2 in rats with artificial chronic stress depression, and enhance the immune function of rats with chronic stress depression. Polygonatum sibiricum also has the effect of improving memory and preventing and treating Alzheimer's disease. Studies have found that compounds in Polygonatum sibiricum extracted with ethanol can significantly improve the memory function of mice with memory acquisition disorders due to scopolamine; other studies have shown that Polygonatum sibiricum polysaccharides can increase the mitochondrial density in the CA1 region of the hippocampus of demented mice and reduce the degree of mitochondrial deformation. Polygonatum sibiricum also has anti-depressant function. Studies have found that Polygonatum sibiricum saponins can correct the autonomous activities and learning and memory abilities of artificially depressed mice and increase the level of monoamine neurotransmitters in the brain. Polygonatum has a certain function of lowering blood sugar. Studies have found that Polygonatum polysaccharide can significantly reduce the blood sugar and serum glycosylated hemoglobin concentration of diabetic mice artificially induced by experiments. Polygonatum polysaccharide can significantly increase the level of insulin and C-peptide in plasma. Studies have also shown that Polygonatum polysaccharide can reduce the blood sugar of rats induced by streptozotocin and eventually lead to diabetes, and can also increase the expression of insulin. The possible mechanism is that it inhibits insulin cell apoptosis and downregulates the expression of caspase-3. Polygonatum can also be used to regulate blood lipids and resist atherosclerosis. Studies have found that Polygonatum polysaccharide can downregulate the high expression of vascular cell adhesion molecule-1 (VCAM-1) in the intima of experimental rabbit atherosclerosis, inhibit the adhesion of inflammatory cells to endothelial cells, and prevent the occurrence and development of vascular endothelial inflammatory response. Experiments have also confirmed that Polygonatum polysaccharide can reduce the total cholesterol and triglyceride content in the blood of mice, and has a significant preventive and therapeutic effect on experimental hyperlipidemia mice.

[0003] The traditional processing method of Polygonatum sibiricum is to steam it nine times and dry it nine times. After steaming it once, dry it in the sun and twist it by hand; steam it again, dry it again, and twist it again; repeat this nine times to turn the raw Polygonatum sibiricum into cooked Polygonatum sibiricum. The product can only be in the form of blocks, filaments or granules and cannot be made into fine powder. The product form is very single, the polysaccharide content is about 7%, and the processing cycle time is about 1 month. The cycle time is long, the energy consumption is high, and the labor consumption is high. It cannot be achieved on a large scale and can only remain on a small scale.

[0004] Therefore, how to provide a method for producing Polygonatum sibiricum powder with a short cycle, high polysaccharide content and scalable production is an urgent problem that technicians in this field need to solve. Summary of the invention

[0005] In view of this, the present invention provides a polygonatum powder and a production method thereof, wherein the polygonatum is cleaned and pulped by enzymolysis, a food-grade enzyme preparation is added, and the enzymolysis is carried out at a certain temperature and time, followed by inactivation and drying to obtain the polygonatum powder.

[0006] The polysaccharide content of the polygonatum powder prepared by enzymatic hydrolysis is high, reaching up to 48.30%; the production cycle is short, and it takes about 3 days to complete; and compared with nine steaming and nine drying, the present invention has low energy consumption and can be mass-produced.

[0007] In order to achieve the above object, the present invention adopts the following technical solution:

[0008] First, the present invention provides a method for producing polygonatum powder, which specifically comprises the following steps:

[0009] S1, take fresh Polygonatum sibiricum tubers, remove the capillary roots and impurities, wash with clean water, and dry;

[0010] S2, grinding the dried Polygonatum cyrtonema tubers with colloid into pulp, adding an appropriate amount of water during the process to obtain a slurry;

[0011] S3, heating the obtained slurry, adding cellulase for enzymolysis, and obtaining enzymolysis solution A;

[0012] Its beneficial effects are as follows: the plant body of Polygonatum sibiricum is mainly composed of fibers, which support its growth. The fibers are mainly composed of cellulose. Cellulase acts on the β-1,4 glucosidic bonds of cellulose, degrading cellulose into short fibers, cellobiose and glucose. At the same time, the cellulose in the cell wall of Polygonatum sibiricum is also enzymolyzed, so that the cell wall is opened, and the nutrients in the cell can be fully released and enter the enzymolysis solution, which is beneficial to the body's absorption and utilization. The cellulose in Polygonatum sibiricum is enzymolyzed, which opens up a convenient channel for the next enzymolysis. Without the blockage of cellulose, the subsequent other enzymes are more conducive to enzymolysis.

[0013] S4, heating the enzymatic hydrolyzate A, adjusting the pH to 4.5-5.0, adding pectinase for enzymatic hydrolysis, and obtaining enzymatic hydrolyzate B;

[0014] Its beneficial effects are as follows: Pectin is one of the main components of plant cell walls and intercellular layers, which binds plant cells together. Pectinase acts on the glycosidic bonds of D-galacturonic acid residues in pectin, breaking pectin molecules and degrading the polysaccharide chains of pectin to generate oligogalacturonic acid and a small amount of galacturonic acid small molecules; after pectin hydrolysis, there is no obstruction from pectin, and the nutrients in the cells are released into the enzymatic solution.

[0015] S5, heating the enzymatic hydrolyzate B, adjusting the pH to 4.0-5.0, adding α-amylase for enzymatic hydrolysis, and obtaining enzymatic hydrolyzate C;

[0016] Its beneficial effects are: Polygonatum contains some starch, and α-amylase can hydrolyze the α-1.4 glucosidic bonds in starch molecules, arbitrarily cutting them into short-chain dextrins of varying lengths and a small amount of low-molecular sugars, turning large molecules into small molecules.

[0017] S6, keeping the temperature of the enzymatic hydrolyzate C unchanged, adjusting the pH to 4.0-5.0, adding saccharifying enzyme for enzymatic hydrolysis, and obtaining enzymatic hydrolyzate D;

[0018] Its beneficial effect is that saccharifying enzyme can gradually hydrolyze short-chain dextrin and low-molecular sugars after being hydrolyzed by amylase into monosaccharides, thereby making the polygonatum sweet.

[0019] S7, adjusting the pH of the enzymatic hydrolysate D to 6.0-7.0, performing enzyme inactivation treatment, and obtaining an enzymatic hydrolysate E;

[0020] S8, drying the obtained enzymatic hydrolyzate E to obtain polygonatum sibiricum powder.

[0021] The pH is adjusted using food grade citric acid and food grade sodium carbonate.

[0022] The water: Polygonatum sibiricum tuber ratio in step S2 is 2.4-3 L: 1 kg.

[0023] The heating in step S3 is 50-55° C.; the cellulase activity is 10,000-500,000 u / g, and the dosage is 0.1%-0.3% of the weight of polygonatum; and the enzymolysis time is 2-4 hours.

[0024] The temperature in step S4 is raised to 55-60° C.; the pectinase activity is 10,000-30,000 u / g, and the dosage is 0.2%-0.3% of the weight of polygonatum; and the enzymolysis time is 4-6 hours.

[0025] The temperature in step S5 is raised to 60-65° C.; the activity of the a-amylase is 10,000 u / g, and the dosage is 0.2%-0.3% of the weight of the polygonatum; and the enzymolysis time is 2-6 hours.

[0026] The saccharifying enzyme activity in step S6 is 200,000 u / g, and the dosage is 0.1% to 0.2% of the weight of polygonatum; the enzymolysis time is 4 to 6 hours.

[0027] The inactivation treatment temperature in step S7 is 80° C. and the time is 20 min.

[0028] The drying process in step S8 is spray drying or air drying.

[0029] In addition, the present invention also claims protection for the polygonatum powder prepared by the above method.

[0030] It can be seen from the above technical scheme that compared with the prior art, the present invention discloses a kind of polygonatum powder and its production method, wherein the polygonatum is washed and pulped, a food-grade enzyme preparation is added, and the polygonatum powder is obtained by enzymolysis. The present invention shortens the production cycle, and the polygonatum powder can be obtained in only 3 days, and the production process is very suitable for large-scale production. The traditional production method of nine steaming and nine drying takes about 30 days, and can only be produced in a small workshop, and the product form can only be shredded or granulated, and cannot be made into powder.

[0031] Fresh Polygonatum sibiricum is edible when eaten raw. Through microscopic examination, fresh Polygonatum sibiricum pectin is composed of crystals, which are pointed and irritate the oral mucosa when entering the mouth, causing discomfort. The product prepared by the present invention is a yellow-brown powder with a moisture content of about 11.5%, slightly sweet, and not numbing. It is powdery under a microscope and has no sharp crystals. This indicates that the present application destroys the crystalline pectin of Polygonatum sibiricum through enzymatic hydrolysis, turning it into fine particles, so it no longer irritates the oral mucosa.

[0032] The product of the present application has a high content of active substances, wherein the polysaccharide content is 16.25%-48.30%, the total saponin content is 3.27%-3.52%, and the total flavonoid content is 7.82mg-8.07mg. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on the provided drawings without paying creative work.

[0034] Figure 1 The morphology of fresh Polygonatum sibiricum slices under a microscope before enzymatic hydrolysis, magnified 640 times.

[0035] Figure 2 The morphology of dried powder of Polygonatum sibiricum after enzymatic hydrolysis under a microscope, magnified 640 times.

[0036] Figure 3 The appearance of Polygonatum sibiricum under a microscope after being steamed using the traditional method, magnified 640 times. DETAILED DESCRIPTION

[0037] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0038] Example 1

[0039] (1) Take fresh Polygonatum sibiricum tubers, remove the capillary roots and impurities, wash with clean water, and dry;

[0040] (2) taking 50 kg of dried Polygonatum sibiricum tubers and grinding them into pulp using a colloid grinder, and adding 150 L of water during the process to obtain a slurry;

[0041] (3) The obtained slurry was heated to 50° C., 150 g of cellulase (10,000 u / g) was added, and enzymolysis was performed for 2 hours to obtain enzymolysis solution A;

[0042] (4) heating the enzymatic hydrolysate A to 55° C., adjusting the pH value to 4.5 with citric acid, adding 150 g of pectinase (10,000 u / g), and performing enzymatic hydrolysis for 4 hours to obtain enzymatic hydrolysate B;

[0043] (5) The temperature of the enzymatic hydrolyzate B was raised to 60° C., the pH was adjusted to 4.5, 150 g of α-amylase (10,000 u / g) was added, and the enzymatic hydrolysis was performed for 4 hours to obtain the enzymatic hydrolyzate C;

[0044] (6) Keeping the temperature of the enzymatic hydrolyzate C at 60°C, adjusting the pH to 4.5, adding 75 g of saccharifying enzyme (200,000 u / g), and performing enzymatic hydrolysis for 4 hours to obtain enzymatic hydrolyzate D;

[0045] (7) adjusting the pH of the enzymatic hydrolysate D to 6.0, heating to 80° C., and inactivating for 20 min to obtain an enzymatic hydrolysate E;

[0046] (8) The obtained enzymatic hydrolyzate E is dried by air at a temperature of 70° C. to obtain polygonatum sibiricum powder.

[0047] Example 2

[0048] (1) Take fresh Polygonatum sibiricum tubers, remove the capillary roots and impurities, wash with clean water, and dry;

[0049] (2) taking 50 kg of dried Polygonatum sibiricum tubers and grinding them into pulp using a colloid grinder, and adding 150 L of water during the process to obtain a slurry;

[0050] (3) The obtained slurry was heated to 50° C., 120 g of cellulase (10,000 u / g) was added, and enzymolysis was performed for 2 hours to obtain enzymolysis solution A;

[0051] (4) The enzymatic hydrolyzate A was heated to 55° C., the pH value was adjusted to 4.5 by citric acid, 120 g of pectinase (10,000 u / g) was added, and enzymatic hydrolysis was performed for 4 hours to obtain enzymatic hydrolyzate B;

[0052] (5) The temperature of the enzymatic hydrolyzate B was raised to 60° C., the pH was adjusted to 4.5, 120 g of α-amylase (10,000 u / g) was added, and the enzymatic hydrolysis was performed for 4 hours to obtain the enzymatic hydrolyzate C;

[0053] (6) Keeping the temperature of the enzymatic hydrolyzate C at 60°C, adjusting the pH to 4.5, adding 60 g of saccharifying enzyme (200,000 u / g), and performing enzymatic hydrolysis for 4 hours to obtain enzymatic hydrolyzate D;

[0054] (7) adjusting the pH of the enzymatic hydrolysate D to 6.0, heating to 80° C., and inactivating for 20 min to obtain an enzymatic hydrolysate E;

[0055] (8) The obtained enzymatic hydrolyzate E is dried by air at a temperature of 70° C. to obtain polygonatum sibiricum powder.

[0056] Example 3

[0057] (1) Take fresh Polygonatum sibiricum tubers, remove the capillary roots and impurities, wash with clean water, and dry;

[0058] (2) taking 50 kg of dried Polygonatum sibiricum tubers and grinding them into pulp using a colloid grinder, and adding 150 L of water during the process to obtain a slurry;

[0059] (3) The obtained slurry was heated to 50° C., 150 g of cellulase (10,000 u / g) was added, and enzymolysis was performed for 3 hours to obtain enzymolysis solution A;

[0060] (4) heating the enzymatic hydrolysate A to 55° C., adjusting the pH value to 4.5 with citric acid, adding 150 g of pectinase (10,000 u / g), and performing enzymatic hydrolysis for 5 hours to obtain enzymatic hydrolysate B;

[0061] (5) The temperature of the enzymatic hydrolyzate B was raised to 60° C., the pH was adjusted to 4.5, 150 g of α-amylase (10,000 u / g) was added, and the enzymatic hydrolysis was performed for 5 hours to obtain the enzymatic hydrolyzate C;

[0062] (6) Keeping the temperature of the enzymatic hydrolyzate C at 60°C, adjusting the pH to 4.5, adding 75 g of saccharifying enzyme (200,000 u / g), and performing enzymatic hydrolysis for 5 hours to obtain enzymatic hydrolyzate D;

[0063] (7) adjusting the pH of the enzymatic hydrolysate D to 6.0, heating to 80° C., and inactivating for 20 min to obtain an enzymatic hydrolysate E;

[0064] (8) The obtained enzymatic hydrolyzate E is dried by air at a temperature of 70° C. to obtain polygonatum sibiricum powder.

[0065] Example 4

[0066] (1) Take fresh Polygonatum sibiricum tubers, remove the capillary roots and impurities, wash with clean water, and dry;

[0067] (2) taking 50 kg of dried Polygonatum sibiricum tubers and grinding them into pulp using a colloid grinder, and adding 120 L of water during the process to obtain a slurry;

[0068] (3) The obtained slurry was heated to 50° C., 50 g of cellulase (500,000 u / g) was added, and enzymolysis was performed for 2 hours to obtain enzymolysis solution A;

[0069] (4) The enzymatic hydrolyzate A was heated to 55°C, the pH value was adjusted to 4.5 by citric acid, 100 g of pectinase (30,000 u / g) was added, and enzymatic hydrolysis was performed for 4 hours to obtain enzymatic hydrolyzate B;

[0070] (5) The temperature of the enzymatic hydrolyzate B was raised to 60° C., the pH was adjusted to 4.5, 100 g of α-amylase (10,000 u / g) was added, and enzymatic hydrolysis was performed for 2 hours to obtain enzymatic hydrolyzate C;

[0071] (6) The temperature of the enzymatic hydrolyzate C was maintained at 60°C, the pH was adjusted to 4.5, 100 g of saccharifying enzyme (200,000 u / g) was added, and enzymatic hydrolysis was performed for 4 hours to obtain enzymatic hydrolyzate D;

[0072] (7) adjusting the pH of the enzymatic hydrolysate D to 6.0, heating to 80° C., and inactivating for 20 min to obtain an enzymatic hydrolysate E;

[0073] (8) The obtained enzymatic hydrolyzate E is dried by air at a temperature of 70° C. to obtain polygonatum sibiricum powder.

[0074] Product Testing

[0075] The shape, moisture, taste, content of main active ingredients, microscopic morphology and other properties of fresh Polygonatum sibiricum and enzymolysis Polygonatum sibiricum prepared in Examples 1-3 of the present application were tested, and compared with the traditional method of steaming Polygonatum sibiricum products. The results are as follows:

[0076]

[0077] Microscopic examination of morphology (640 times) results are shown in Figure 1-3 As can be seen from the figure, fresh Polygonatum is made of sharp crystalline granular pectin. After enzymatic hydrolysis, the product is in powder form without sharp crystals. After traditional steaming, the finished product is in shredded or granular form without sharp crystals. Fresh Polygonatum is numbing when eaten raw because the pectin of fresh Polygonatum is crystalline, and the crystals are pointed. When entering the mouth, they irritate the oral mucosa and cause discomfort. Through enzymatic hydrolysis by four enzymes, the crystalline pectin is destroyed and turned into fine particles, so it no longer irritates the oral mucosa.

[0078] In addition, it can be seen from the data in the table that the content of polysaccharides, total saponins, and total flavonoids in the polygonatum powder prepared by the enzymatic hydrolysis method of the present application is higher than that in the polygonatum product obtained by the traditional steaming method. Moreover, by comparing Example 4 with Examples 1-3, it can be seen that increasing the activity of the enzyme used for enzymatic hydrolysis can significantly increase the polysaccharide content of the obtained polygonatum powder. This shows that the polygonatum powder prepared by the enzymatic hydrolysis method has a higher content of active substances, which further shows that it has a better medicinal effect.

[0079] In this specification, each embodiment is described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the embodiments can be referred to each other. For the device disclosed in the embodiment, since it corresponds to the method disclosed in the embodiment, the description is relatively simple, and the relevant parts can be referred to the method part.

[0080] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A method for producing polygonatum powder, characterized in that: The specific steps include: S1, take fresh Polygonatum sibiricum tubers, remove the capillary roots and impurities, wash with clean water, and dry; S2, grinding the dried Polygonatum cyrtonema tubers with colloid into pulp, adding an appropriate amount of water during the process to obtain a slurry; S3, heating the obtained slurry, adding cellulase for enzymolysis, and obtaining enzymolysis solution A; S4, heating the enzymatic hydrolyzate A, adjusting the pH to 4.5-5.0, adding pectinase for enzymatic hydrolysis, and obtaining enzymatic hydrolyzate B; S5, heating the enzymatic solution B, adjusting the pH to 4.0-5.0, adding α-amylase for enzymatic hydrolysis, and obtaining enzymatic solution C; S6, keeping the temperature of the enzymatic hydrolyzate C unchanged, adjusting the pH to 4.0-5.0, adding saccharifying enzyme for enzymatic hydrolysis, and obtaining enzymatic hydrolyzate D; S7, adjusting the pH of the enzymatic hydrolysate D to 6.0-7.0, performing enzyme inactivation treatment, and obtaining an enzymatic hydrolysate E; S8, drying the obtained enzymatic hydrolyzate E to obtain polygonatum sibiricum powder.

2. The method for producing polygonatum sibiricum powder according to claim 1, characterized in that: The pH is adjusted using food grade citric acid and food grade sodium carbonate.

3. The method for producing polygonatum sibiricum powder according to claim 1, characterized in that: The water: Polygonatum sibiricum tuber ratio in step S2 is 2.4-3 L: 1 kg.

4. The method for producing polygonatum sibiricum powder according to claim 1, characterized in that: The heating temperature in step S3 is 50-55° C.; the cellulase activity is 10,000-500,000 u / g, and the dosage is 0.1%-0.3% of the weight of polygonatum; and the enzymolysis time is 2-4 hours.

5. The method for producing polygonatum sibiricum powder according to claim 1, characterized in that: The heating temperature in step S4 is 55-60° C.; the pectinase activity is 10,000-30,000 u / g, and the dosage is 0.2%-0.3% of the weight of polygonatum; and the enzymolysis time is 4-6 hours.

6. The method for producing polygonatum sibiricum powder according to claim 1, characterized in that: The heating temperature in step S5 is 60-65° C.; the activity of the a-amylase is 10,000 u / g, and the dosage is 0.2%-0.3% of the weight of the polygonatum; and the enzymolysis time is 2-6 hours.

7. The method for producing polygonatum sibiricum powder according to claim 1, characterized in that: The saccharifying enzyme activity in step S6 is 200,000 u / g, and the dosage is 0.1% to 0.2% of the weight of polygonatum; the enzymolysis time is 4 to 6 hours.

8. The method for producing polygonatum sibiricum powder according to claim 1, characterized in that: The inactivation treatment temperature in step S7 is 80° C. and the time is 20 min.

9. The method for producing polygonatum sibiricum powder according to claim 1, characterized in that: The drying process in step S8 is spray drying or air drying.

10. A polygonatum powder, characterized in that: The product is prepared by the production method described in any one of claims 1 to 9.

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