Fungus composition for preventing and treating panax notoginseng root rot and application thereof

Abstract

The invention discloses a fungal composition for preventing and treating root rot of panax notoginseng. The fungal composition is prepared from Trichoderma harzianum SQ T8 and Trichoderma harzianum SQ T8, and the Trichoderma harzianum SQ T8 can be used for preventing and treating root rot of panax notoginseng. The Panax notoginseng root rot can be effectively prevented and treated, a new alternative strain is provided for biological prevention and treatment of the Panax notoginseng root rot, chemical pesticide input in the Panax notoginseng management process is reduced, ecological environment protection is enhanced, the Panax notoginseng pesticide residue heavy metal pollution risk is reduced, cost is saved, the monomer saponin content of Panax notoginseng can be effectively increased, and the Panax notoginseng quality is guaranteed.

Description

Technical Field

[0001] The invention relates to the technical field of plant beneficial bacteria compositions, and in particular to a composition for preventing and treating Panax notoginseng root rot fungi and an application thereof. Background Art

[0002] Panax notoginseng is a perennial herb of the genus Panax, Araliaceae. Its medicinal parts are roots and rhizomes. It is a precious and authentic medicinal material unique to my country, and is known as "golden and incomparable" and "magic grass of the south". The chemical components of Panax notoginseng include non-saponins and saponins. Non-saponins mainly include notoginseng, proteins, polysaccharides, flavonoids, trace elements and some other components. Saponins are the main chemical components and medicinal active ingredients of Panax notoginseng. They have a good therapeutic effect on coronary artery disease and cardiovascular and cerebrovascular diseases, and have a certain preventive effect on cancer. Therefore, the saponin content is often used to evaluate the quality of Panax notoginseng, a traditional Chinese medicine.

[0003] Panax notoginseng is a perennial root plant that likes shade and humidity. Large-scale monoculture for many years is very likely to be infected by pathogens in the soil. Root rot is the most serious disease in the growth process of Panax notoginseng. Its main pathogens are: Fusarium oxysporum, Fusarium solani, Cylindrocarpondestructans, etc. Root rot can cause a 5% to 20% reduction in Panax notoginseng yield, and in severe cases it can reach more than 70%. The losses caused by root rot account for 70% to 85% of all Panax notoginseng diseases, and has become a serious obstacle to the development of Panax notoginseng planting industry.

[0004] At present, the main method for preventing and controlling Panax notoginseng root rot is through spraying chemical agents, but the long-term use of chemical pesticides will cause environmental pollution, ecosystem destruction, the development of pathogen resistance, and even bring hazards such as pesticide residues and heavy metal pollution to Panax notoginseng. Therefore, it is of great significance to reasonably reduce the use of pesticides and find green and environmentally friendly prevention and control methods to prevent and control Panax notoginseng root rot. Summary of the invention

[0005] The purpose of the present invention is to provide a fungal composition for preventing and controlling Panax notoginseng root rot and its application, in view of the current serious occurrence of Panax notoginseng root rot, and the unsatisfactory effects of the prevention and control measures of pesticides, crop rotation and soil, which also lead to the problems of reduced medicinal material quality and environmental pollution.

[0006] In order to solve the above technical problems, the present invention adopts the following technical solutions:

[0007] A Trichoderma harzianum, wherein the Trichoderma harzianum is Trichoderma harzianum SQ T8, which is used to inhibit Fusarium oxysporum, Fusarium solani, and Cylindrospermum destructans;

[0008] It is deposited in the China General Microbiological Culture Collection Center with the deposit number CGMCC No.41468; among them, the deposit time of the deposit number CGMCC No.41468 is July 19, 2024, and the classification name is Trichoderma harzianum.

[0009] A Mortierella, wherein the Mortierella is Mortierella alpina SQ T10,

[0010] Used to inhibit Fusarium oxysporum, Fusarium solani, and Cylindrospermum destructans;

[0011] It is deposited in the China General Microbiological Culture Collection Center with the deposit number CGMCC No.41410; among them, the deposit time of the deposit number CGMCC No.41410 is July 19, 2024, and the classification name is Mortierella alpina.

[0012] A fungal composition for preventing and treating Panax notoginseng root rot, comprising: Trichoderma harzianum SQ T8 and Trichoderma harzianum SQ T8.

[0013] Application of a fungus composition for preventing and controlling root rot of Panax notoginseng to promoting the growth of Panax notoginseng.

[0014] According to the use of a composition for preventing and controlling Panax notoginseng root rot fungus in promoting the accumulation of saponins in Panax notoginseng roots.

[0015] Further: the application method is:

[0016] The seed liquid of fungi SQ T8 and SQ T10 were inoculated into PDA medium respectively, and inverted in an incubator for activation; after the strains were activated, the strains were selected and inoculated into PDA liquid culture medium for cultivation;

[0017] The culture medium was removed by centrifugation, and the bacteria were resuspended in sterile water. The dilution multiple was determined and the concentrations of the strains were 10 8 cfu / mL of mixed bacterial solution;

[0018] After Panax notoginseng emerges, irrigate with mixed bacterial solution.

[0019] Further: the Panax notoginseng that promotes the growth of Panax notoginseng is selected from two-year-old Panax notoginseng or three-year-old Panax notoginseng.

[0020] Further: The method for determining the dilution multiple is:

[0021] According to the bacterial solution OD 600The standard curve was drawn based on the value and the bacterial concentration obtained by the plate counting method. 600 The value determines the dilution factor.

[0022] Furthermore: 200 ml of the mixed bacterial solution is irrigated into the roots of each Panax notoginseng plant.

[0023] Further: In the application, the notoginseng saponin determination is performed by selecting the main root of Panax notoginseng, drying at a constant temperature, powdering, sieving, and then performing the determination.

[0024] Compared with the prior art, the present invention has at least one of the following beneficial effects:

[0025] (1) The beneficial bacteria provided by the present invention have an inhibitory effect on Fusarium oxysporum, Fusarium solani, and Cylindrospermum destructans.

[0026] (2) The beneficial bacteria combination provided by the present invention can promote the growth of Panax notoginseng and increase the content and accumulation of each saponin monomer and total saponin in the main root of Panax notoginseng, and has a positive effect on the formation of Panax notoginseng quality.

[0027] (3) The present invention is based on the rhizosphere soil microorganisms of Panax notoginseng, and separates and identifies SQ T8 and SQ T10, screens the antibacterial activity of the pathogenic bacteria of Panax notoginseng root rot, prepares a mixed bacterial liquid of SQ T8 and SQ T10 for field tests, and obtains a fungal combination that can prevent and control Panax notoginseng root rot and improve the quality of Panax notoginseng. It can effectively prevent and control Panax notoginseng root rot, provide new strains for biological control of Panax notoginseng root rot, reduce the input of chemical pesticides in the management process of Panax notoginseng, enhance the protection of the ecological environment, reduce the risk of heavy metal pollution caused by agricultural residues of Panax notoginseng, save costs, and effectively increase the content of Panax notoginseng monomer saponin, ensure the quality of Panax notoginseng, and at the same time provide new materials for the development of medicinal microbial agents. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 The colony diagrams of strains SQ T8 and SQ T10 of the present invention are shown in FIG.

[0029] Figure 2 The results of the plate confrontation experiment of the strain SQ T8 of the present invention with Fusarium oxysporum, Fusarium solani and Cylindrosporium destructans are shown in FIG. A is a colony photograph of the Fusarium oxysporum control group; B is a colony photograph of the Fusarium oxysporum treatment group; C is a colony photograph of the Fusarium solani control group; D is a colony photograph of the Fusarium solani treatment group; E is a colony photograph of the Cylindrosporium destructans control group; and F is a colony photograph of the Cylindrosporium destructans treatment group.

[0030] Figure 3The results of the plate confrontation experiment of the strain SQ T10 of the present invention with Fusarium oxysporum, Fusarium solani and Cylindrosporium destructans are shown in FIG. A is a colony photograph of the Fusarium oxysporum control group; B is a colony photograph of the Fusarium oxysporum treatment group; C is a colony photograph of the Fusarium solani control group; D is a colony photograph of the Fusarium solani treatment group; E is a colony photograph of the Cylindrosporium destructans control group; and F is a colony photograph of the Cylindrosporium destructans treatment group.

[0031] Figure 4 Schematic diagram of the evolutionary tree of strain SQ T8 of the present invention.

[0032] Figure 5 Schematic diagram of the evolutionary tree of strain SQ T10 of the present invention.

[0033] Figure 6 The relationship between the content and cumulative amount of each saponin monomer in the main root of Panax notoginseng by the mixed fermentation liquid of strains SQ T8 and SQ T10 of the present invention; wherein A is Panax notoginseng saponin R 1 Content; B is ginsenoside Rg 1 Content; C is ginsenoside Rb 1 content; D is ginsenoside Rd content; E is notoginsenoside R 1 Cumulative amount; F is ginsenoside Rg 1 Cumulative amount; G is ginsenoside Rb 1 Cumulative amount; H is the cumulative amount of ginsenoside Rd.

[0034] Figure 7 The figure is a relationship diagram of the total saponin content and cumulative amount in the main root of Panax notoginseng by the mixed fermentation liquid of the strains SQ T8 and SQ T10 of the present invention; wherein A is the total saponin content; and B is the total saponin cumulative amount. DETAILED DESCRIPTION

[0035] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0036] Example 1

[0037] 1) Separation and purification

[0038] The rhizosphere soil of three-year-old Panax notoginseng was collected from Yunnan. After removing the stones from the rhizosphere soil, 5 g was weighed and put into a triangular flask containing 45 mL of sterile water. The triangular flask was placed in a shaker at 25°C, 180 r / min, and 30 min to obtain a 10-fold diluted soil suspension. After the 10-fold diluted soil suspension was allowed to stand for 1 hour, 1 ml of the upper soil suspension was taken and diluted 1000 times, 10,000 times, and 100,000 times with sterile water to obtain a 1000-fold, 10,000-fold, and 100,000-fold soil suspension. 10000 times soil suspension and 100000 times soil suspension, and then evenly spread 1000 times soil suspension diluted with sterile water on PDA medium, 10000 times soil suspension diluted with sterile water on PDA medium, and 100000 times soil suspension diluted with sterile water on PDA medium, and all PDA mediums are placed in a 30°C incubator and inverted for 7 to 14 days. Cultivate and observe, after the colonies grow in the culture medium, pick out the single colonies with different morphologies and inoculate them into new PDA culture medium, invert and culture for 2 days in a 30°C incubator, and perform streak purification and separation, and repeat the streak purification and separation many times until a purified single strain is obtained.

[0039] Dispense 10 mL of PDA culture medium into 18 mm × 180 mm test tubes, seal with gauze, and sterilize at 121°C for 20 min. After sterilization, place the tubes into a slope about 1 / 2 the length of the test tubes, cool and solidify, and set aside.

[0040] The purified single strains were inoculated on the prepared PDA solid slant medium, plugged with sterilized rubber stoppers, and transferred to a 4°C refrigerator for storage after the bacteria fully grew, as single strain seed liquid. In order to ensure the purity of the single strain seed liquid, the strains were transplanted every 4 months. The single strain seed liquid was used for the antibacterial activity of Panax notoginseng root rot pathogens and for inoculation each time the mixed bacterial liquid was prepared.

[0041] The composition of PDA medium is: potato extract powder 12g / L, glucose 20g / L, agar powder 15g / L, add water to 1L, pH natural. After preparation, sterilize at 120℃ for 20 minutes.

[0042] The composition of PDA liquid culture medium is: potato extract powder 12g / L and glucose 20g / L, add water to 1L, pH is natural. After preparation, sterilize at 120℃ for 20 minutes.

[0043] 2) Screening of antibacterial activity of strains against pathogens of Panax notoginseng root rot

[0044] 2.1 Methods:

[0045] The seed liquid of each single strain was taken and inoculated on PDA medium, and then incubated in an incubator at 30°C for 2 days to obtain activated single strain colonies for later use.

[0046] Experimental group: The pathogens of Panax notoginseng root rot, Fusarium oxysporum, Fusarium solani and Cylindrospermum destructum were used as target bacteria and inoculated in the center of PDA culture medium. The activated single strains were inoculated at a point 2 cm away from the center of the plate and cultured in a 30°C incubator for 5 days. Each single strain was repeated 5 times.

[0047] Control group: The pathogens of Panax notoginseng root rot, Fusarium oxysporum, Fusarium solani, and Cylindrospermum destructum were used as target bacteria, respectively. The target bacteria were inoculated in the center of the PDA culture medium, cultured in a 30°C incubator for 5 days, and repeated 5 times.

[0048] After 5 days of culture, the treated and control groups were taken out, and the diameters of the pathogenic fungal colonies in the PDA medium were measured, and the inhibition rate was calculated. Inhibition rate % = (control target colony diameter - treatment group target colony diameter) / control group target colony diameter × 100%.

[0049] 2.2 Measurement results:

[0050] Among the purified single strains, only SQ T8 (single strain 8) and SQ T10 (single strain 10) had an inhibitory effect on the pathogen of Panax notoginseng root rot. The specific inhibitory effects were as follows:

[0051] The colonies of Fusarium oxysporum in the control group were as follows Figure 2 As shown in A and 3A, the colony has irregular edges, a fluffy surface, and is off-white. Figure 2 As shown in B and 3B, after single strains SQ T8 and SQ T10 were streaked and cultured next to the pathogenic target bacteria, the growth of Fusarium oxysporum was inhibited, and an obvious inhibition zone was formed on the side close to the colonies of strains SQ T8 and SQ T10, and the color of the pathogenic bacteria colonies was lighter than that of the blank group. According to Table 1, the diameter of the single colony of Fusarium oxysporum in the control group was 30 mm, and the diameter of the inhibition zone of SQ T8 and SQ T10 against Fusarium oxysporum was 10.2 mm and 25.7 mm, respectively, and the inhibition rates were 66.0% and 14.3%, respectively.

[0052] The colonies of Fusarium solani in the control group were Figure 2 As shown in C and 3C, the colony edges are complete, symmetrical, and gray in color. Figure 2 As shown in D and 3D, after the single strains SQ T8 and SQ T10 were streaked and cultured next to the pathogenic target bacteria, the growth of Fusarium solani was inhibited, and an obvious inhibition zone was formed on the side close to the colonies of the single strains SQ T8 and SQ T10, and the color of the pathogenic bacteria colonies was lighter than that of the blank group. According to Table 1, the single colony diameter of Fusarium solani in the control group was 63.3 mm, and the diameters of the inhibition zones of SQ T8 and SQ T10 against Fusarium oxysporum were 12.3 mm and 20.8 mm, respectively, and the inhibition rates were 80.6% and 67.1%, respectively.

[0053] The control group destroyed the colonies of Cylindrospermum Figure 2 E, 3E, the colony edge is complete, covering the central area of ​​the culture medium, and the colony is symmetrical as a whole. Figure 2 As shown in F and 3F, after single strains SQ T8 and SQ T10 were streaked and cultured next to the pathogenic target bacteria, the growth of Cylindrospermum destructans was inhibited, and an obvious inhibition zone was formed on the side close to the colonies of single strains SQ T8 and SQ T10, among which the color of the pathogenic bacteria colony close to SQ T8 was lighter than that of the blank group. According to Table 1, the single colony diameter of Fusarium solani in the CK group was 23.8 mm, and the diameters of the inhibition zones of SQ T8 and SQ T10 against Fusarium oxysporum were 11.5 mm and 17.7 mm, respectively, and the inhibition rates were 51.7% and 25.6%, respectively.

[0054] From the above, it can be concluded that single strains SQ T8 and SQ T10 have the effect of inhibiting the growth, development and proliferation of Fusarium oxysporum, Fusarium solani and Columnar spores. Among them, the inhibitory effect of SQ T8 on the three pathogens of Panax notoginseng root rot is stronger than that of SQ T10, so the effect of preventing and controlling Panax notoginseng root rot is stronger than that of SQ T10; the inhibitory effect of SQ T10 on Fusarium solani is stronger than that on Fusarium oxysporum and Columnar spores.

[0055] Table 1 Antibacterial activity against Panax notoginseng pathogenic fungi

[0056]

[0057] 3) Identification

[0058] 3.1 Observation of morphological characteristics: The seed liquid of single strains SQ T8 and SQ T10 were inoculated on PDA plates, and then incubated in an incubator at 30°C for 2 days to obtain single strain colonies. The colonies formed by SQ T8 and SQ T10 are as follows: Figure 3 As shown, SQ T8 appeared green on the PDA plate, with a filamentous surface, neat edges and a round shape; SQ T10 colonies appeared gray on the PDA plate, with filamentous layers and irregular edges.

[0059] 3.2 Identification of antagonistic functional bacteria: The total fungal DNA of single strains SQ T8 and SQ T10 was extracted using the Spint Kit for Soil (MPBIO) kit, and PCR amplification was performed using the universal fungal primers ITS1 / ITS2. After gel electrophoresis, the sequences were sent to Bioengineering Co., Ltd. for sequencing, and the full lengths of the sequences were 594 bp and 650 bp, respectively. The obtained sequences were submitted to the GenBank database for Blast analysis and comparison, and it was found that the strains with higher homology to SQ T8 and SQ T10 belonged to Trichodermaharzianum strain MR19 and Mortierella alpina isolate OVR6, respectively. The phylogenetic tree was constructed using MEG7.0 software, as shown in Figure 2. Figure 4 As shown, single strains SQ T8 and SQ T10 were in the same branch as Trichoderma harzianum strain MR19 and Mortierella alpina isolate OVR6, respectively.

[0060] Based on the above morphological characteristics analysis, ITS analysis and phylogenetic tree analysis, single strains SQ T8 and SQ T10 were identified as M. harzianum and M. alpina, respectively.

[0061] 4) Field application of SQ T8 and SQ T10 strains to control Panax notoginseng root rot

[0062] 4.1 Preparation method of bacterial solution of SQ T8 and SQ T10 mixture:

[0063] Take the seed liquid of fungi SQ T8 and SQ T10 and inoculate them into PDA medium respectively. Incubate them upside down in an incubator at 30℃ for 2 days to activate them. After activating the strains, pick the strains and inoculate them into PDA liquid culture medium. Incubate them at 30℃ and 180r / min for 2 days. Then, centrifuge the culture medium at 5000r / min to remove the culture medium. Resuspend the bacteria in an appropriate amount of sterile water. 600 The standard curve of the three strains was drawn based on the value and the bacterial concentration obtained by the plate count method. 600 The dilution multiple was determined by the value, and the final concentration of the strains was 10 8 cfu / mL of mixed bacterial solution for later use.

[0064] 4.2 Field use:

[0065] 4.2.1 Field test location and materials: The experiment was set up in Qiubei County and Shilin County, and the test materials were two-year-old or three-year-old Panax notoginseng.

[0066] 4.2.2 Test methods

[0067] In the field experiment, two groups were set up, the experimental group and the CK group, with 3 replicates in each group and 100 Panax notoginseng plants in each replicate.

[0068] The experimental group was treated with 10 8 cfu / mL of SQ T8 and SQ T10 mixed bacterial solution, 200 ml of the mixed bacterial solution was irrigated into the roots of each Panax notoginseng plant; for CK treatment, 200 ml of distilled water was irrigated into the roots of each Panax notoginseng plant; after an interval of 1 month, the above method was repeated once.

[0069] No root rot prevention chemicals were used during the growth period, and other management was carried out according to conventional management. When the three-year-old Panax notoginseng was harvested, agricultural indicators were measured and the incidence of Panax notoginseng root rot was counted. After the agronomic traits were measured, the main roots of Panax notoginseng in each group were dried at a constant temperature of 45°C, powdered, and passed through a 60-mesh sieve for the determination of Panax notoginseng saponins.

[0070] Disease incidence = number of diseased plants / number of investigated plants*100%;

[0071] Disease control effect = (disease incidence in the control area - disease incidence in the control area) / disease incidence in the control area * 100%.

[0072] 4.2.3 Test results:

[0073] (1) Results of the effects of SQ T8 and SQ T10 compositions on the incidence of Panax notoginseng root rot:

[0074] The mixture of SQ T8 and SQ T10 can effectively prevent and control the occurrence of Panax notoginseng root rot (see Table 2).

[0075] Table 2. Effect of SQ T8 and SQ T10 mixed bacterial liquid on the prevention and treatment of Panax notoginseng root rot

[0076]

[0077] It can be seen from Table 2 that the field control efficacy of using SQ T8 and SQ T10 mixed bacterial liquid to control Panax notoginseng root rot can reach more than 70%.

[0078] (2) Effects of SQ T8 and SQ T10 combination bacteria on the growth of Panax notoginseng Results:

[0079] The effects of SQ T8 and SQ T10 combined bacterial liquid on promoting the growth of Panax notoginseng are shown in Table 3.

[0080] Table 3 Effect of SQ T8 and SQ T10 mixed bacterial solution on the growth of Panax notoginseng

[0081]

[0082] Note: Different letters in the same column of the table indicate significant differences in the same origin (P<0.05).

[0083] After inoculation with the combined bacterial liquid of SQ T8 and SQ T10, the height of Panax notoginseng was significantly increased by 9%-27%, the fresh weight of the aboveground part by 18%-22%, the fresh weight of the underground part by 8%-12% and the dry weight of the underground part by 4%-5%.

[0084] (3) The effects of SQ T8 and SQ T10 combination on the percentage and cumulative amount of notoginseng saponins:

[0085] The contents of each monomer saponin in the main root of Panax notoginseng treated with SQ T8 and SQ T10 combined bacterial liquid were higher than those in the control group ( Figure 6 AD), among which notoginsenoside R 1 and ginsenoside Rg 1 , Rb 1 The contents of Rd and Rd in the main roots of Panax notoginseng treated with the mixed solution of bacterial liquid increased by 37.80%, 5.26%, 39.28% and 88.58% respectively compared with those in the control group. 1 and ginsenoside Rg 1 , Rb 1 The contents of Rd and Rd increased by 93.68%, 28.22%, 24.14% and 27.07% compared with the control group. The treatment with SQ T8 and SQ T10 mixed bacterial solution had a significant effect on the accumulation of each monomer saponin in the main root of Panax notoginseng ( Figure 6 EH), among which the mixed bacterial solution treatment group was compared with the control group, the notoginseng saponin R in the main root of Panax notoginseng in Qiubei area 1 , Ginsenoside Rg 1 , Rb 1 The accumulation of notoginseng saponins in the main root of the Shilin area treatment group was significantly higher than that in the control group, among which notoginseng saponin R 1 , Ginsenoside Rg 1 , Rb 1 The accumulation of notoginseng saponins in the main root of Panax notoginseng was increased by 129.21%, 35.27%, 30.87% and 33.86% respectively compared with the control group. The above results showed that the mixed bacterial solution of strains SQ T8 and SQ T10 had a good effect on increasing the accumulation of each monomer saponin in the main root of Panax notoginseng, especially notoginseng saponin R 1 and ginsenoside Rd.

[0086] (4) Effects of SQ T8 and SQ T10 combination bacteria on the percentage and cumulative amount of total saponins of Panax notoginseng:

[0087] The contents of total saponins in the main roots of Panax notoginseng treated with SQ T8 and SQ T10 combined bacterial solutions were higher than those in the control group ( Figure 7 A), among which the content of total saponins in the main roots of Panax notoginseng treated with mixed bacterial solution in Shilin area reached 91.03-108.99 mg / g, significantly higher than 69.69-98.99 mg / g in the control group. The cumulative amount of total saponins in the main roots of Panax notoginseng treated with SQ T8 and SQ T10 mixed bacterial solution was significantly higher than that in the control group ( Figure 7 B). The cumulative amount of total saponins in the main roots of Panax notoginseng in the treatment group in Qiubei area was 1501.51 mg / plant, significantly higher than 1152.06 mg / plant in the control group. The cumulative amount of total saponins in the main roots of Panax notoginseng in the treatment group in Shilin area was 1214.49 mg / plant, significantly higher than 868.85 mg / plant in the control group. The above results show that the mixed bacterial solution of single strains SQ T8 and SQ T10 has a good effect on increasing the cumulative amount of total saponins in the main roots of Panax notoginseng. (Saponin accumulation = underground dry weight * saponin percentage, expressed in mg / plant)

[0088] 4.2.4 Conclusion: The mixed solution of SQ T8 and SQ T10 isolated from the rhizosphere soil of Panax notoginseng has the effect of inhibiting Fusarium oxysporum, Fusarium solani and Columnar spores that cause root rot of Panax notoginseng, promoting the growth of Panax notoginseng and increasing the content and accumulation of saponins in the main root of Panax notoginseng. The inhibition rates of strain SQ T8 against Fusarium oxysporum, Fusarium solani and Columnar spores that cause root rot of Panax notoginseng were 66.1%, 80.5% and 51.7%, respectively, and the inhibition rates of strain SQ T10 against Fusarium oxysporum, Fusarium solani and Columnar spores that cause root rot of Panax notoginseng were 14.4%, 67.1% and 25.9%, respectively. The results of field tests show that after inoculation with the mixed bacterial agent of SQ T8 and SQ T10, the control effect of Panax notoginseng root rot is higher than 70%, and the height of Panax notoginseng plants can be significantly increased by 27%, the fresh weight of the aboveground part increases by 18%-22%, the fresh weight of the underground part increases by 8%-12%, and the dry weight of the underground part increases by 4%-5%. It also has a good effect on increasing the content and accumulation of each saponin monomer in the main root of Panax notoginseng, especially Panax notoginseng saponin R1 and ginsenoside Rd. The strains SQ T8 and SQ T10 in the present invention have the effect of inhibiting root rot Fusarium oxysporum, Fusarium solani and Columnar spores of destructive fungi, and the mixed bacterial liquid of the two strains also has the effect of promoting the growth of Panax notoginseng and improving the quality of Panax notoginseng. The present invention provides a new strain for the control of root rot of medicinal plants, and also provides a new material for the development of medicinal microbial agents.

[0089] Although the present invention is described herein with reference to a number of illustrative embodiments of the present invention, it will be appreciated that those skilled in the art may devise many other modifications and implementations that fall within the scope and spirit of the principles disclosed herein. More specifically, within the scope of the present disclosure, drawings, and claims, a variety of variations and modifications may be made to the components and / or layout of the subject combination layout. In addition to the variations and modifications made to the components and / or layout, other uses will also be apparent to those skilled in the art.

Claims

1. A Trichoderma harzianum, characterized in that: The Trichoderma harzianum is Trichoderma harzianum SQ T8, which is used to inhibit Fusarium oxysporum, Fusarium solani, and Cylindrospermum destructans; It is deposited in China General Microbiological Culture Collection Center with the deposit number CGMCC No.41468.

2. A Mortierella, characterized in that: The Mortierella alpina is Mortierella alpina SQ T10, Used to inhibit Fusarium oxysporum, Fusarium solani, and Cylindrospermum destructans; It is deposited in China General Microbiological Culture Collection Center with the deposit number CGMCC No.41410.

3. A fungal composition for preventing and treating Panax notoginseng root rot, characterized in that: The fungal composition comprises: the Trichoderma harzianum SQ T8 according to claim 1 and the Trichoderma harzianum SQ T8 according to claim 2.

4. Use of the composition for preventing and controlling root rot fungi of Panax notoginseng as claimed in claim 3 in promoting the growth of Panax notoginseng.

5. Use of the composition for preventing and controlling root rot fungi of Panax notoginseng according to claim 3 in promoting the accumulation of saponins in Panax notoginseng roots.

6. The application method according to claim 4, characterized in that: The application method is: The seed liquid of fungi SQ T8 and SQ T10 were inoculated into PDA medium respectively, and inverted in an incubator for activation; after the strains were activated, the strains were selected and inoculated into PDA liquid culture medium for cultivation; The culture medium was removed by centrifugation, and the bacteria were resuspended in sterile water. The dilution multiple was determined and the concentrations of the strains were 10 8 cfu / mL of mixed bacterial solution; After Panax notoginseng emerges, irrigate with mixed bacterial solution.

7. The application method according to claim 6, characterized in that: The Panax notoginseng that promotes the growth of Panax notoginseng is selected from biennial Panax notoginseng or triennial Panax notoginseng.

8. The application method according to claim 6, characterized in that: The method for determining the dilution multiple is: According to the bacterial solution OD 600 The standard curve was drawn based on the value and the bacterial concentration obtained by the plate counting method. 600 The value determines the dilution factor.

9. The application method according to claim 6, characterized in that: Each Panax notoginseng plant was irrigated with 200 ml of the mixed bacterial solution.

10. The use according to claim 4, characterized in that: In the application, the main root of Panax notoginseng is selected for the determination of Panax notoginseng saponins, which is dried at a constant temperature, powdered, sieved, and then determined.

Images