Phytobacterium plantarum YDJ-03 and application thereof

Abstract

The invention provides a lactobacillus plantarum YDJ-03 and an application of the lactobacillus plantarum YDJ-03. According to the invention, the plant lactobacillus YDJ-03 is found for the first time, and the plant lactobacillus YDJ-03 can effectively reduce the malondialdehyde level of the body liver, improve the SOD (superoxide dismutase) activity of the body liver, reduce lipid peroxidation of the body, reduce the serum uric acid level of the body, reduce the serum creatinine level of the body and relieve kidney injury; the compound can be used for preparing food or medicines for preventing and treating kidney injury, protecting liver, regulating fat, protecting intestinal tracts, resisting oxidation and resisting inflammation, and has a relatively great application prospect.

Description

Technical Field

[0001] The invention relates to the technical field of microorganisms, and in particular to a plant lactobacillus YDJ-03 and an application thereof. Background Art

[0002] The intestinal flora is a community of microorganisms living in the digestive tract of humans and animals. In recent years, the regulatory role of the intestinal flora on the host's energy metabolism has gradually been recognized by the academic community. Studies have shown that there is a significant correlation between metabolic syndrome and changes in the intestinal flora. For example, the intestinal Bacteroidetes of obese people are significantly lower than those of normal people, while the abundance of Firmicutes increases. In addition, diabetes, hyperlipidemia and hypertension are all intricately related to the intestinal flora, which is comprehensively manifested as an increase in conditional pathogens and a decrease in beneficial bacteria.

[0003] Probiotics refer to sufficient live microorganisms that are beneficial to the health of the host. Currently recognized ones include Lactobacillus, Bifidobacterium, Streptococcus, etc. Probiotics enhance the health of the host and improve metabolic syndrome through their own metabolites and fermentation of food. Although there are many probiotic products at home and abroad, the quality varies, and the functions of most probiotic products lack sufficient data support. Summary of the invention

[0004] In view of the defects of the prior art, the present invention provides a plant lactobacillus YDJ-03 and its application. The present invention first discovered a plant lactobacillus YDJ-03, which can effectively reduce the level of malondialdehyde in the liver of the body, improve the activity of SOD in the liver of the body, reduce lipid peroxidation in the body, reduce the level of serum uric acid in the body, reduce the level of serum creatinine in the body, and alleviate kidney damage. It can be used to prepare foods or medicines for preventing and treating kidney damage, protecting the liver, regulating fat, protecting the intestines, and anti-oxidation and anti-inflammatory, and has great application prospects.

[0005] First of all, one of the purposes of the present invention is to provide a strain of Lactobacillus plantarum YDJ-03, which was preserved in the General Microbiology Center of the China Culture Collection Administration on July 27, 2023, and the preservation address is the Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, with the preservation number CGMCCNO: 28037, and the classification name is Lactiplantibacillus plantarum.

[0006] One of the purposes of the present invention is to provide a strain of Lactobacillus plantarum YDJ-03 for use in preparing products for preventing and treating kidney damage, protecting the liver, regulating fat, protecting the intestines, and resisting oxidation and inflammation.

[0007] Preferably, the application is that Lactobacillus plantarum YDJ-03 can effectively reduce the level of malondialdehyde in the liver of the body.

[0008] Preferably, the application is that Lactobacillus plantarum YDJ-03 can effectively enhance the activity of SOD in the liver of the body.

[0009] Preferably, the application is that Lactobacillus plantarum YDJ-03 can effectively reduce lipid peroxidation in the body.

[0010] Preferably, the application is that Lactobacillus plantarum YDJ-03 can effectively reduce the body's serum uric acid level.

[0011] Preferably, the application is that Lactobacillus plantarum YDJ-03 can effectively reduce the body's serum creatinine level.

[0012] Preferably, the application is that Lactobacillus plantarum YDJ-03 can effectively alleviate kidney damage.

[0013] One of the purposes of the present invention is to provide a product for preventing and treating kidney damage, protecting the liver, regulating fat, protecting the intestine, anti-oxidation and anti-inflammatory, wherein the product uses Lactobacillus plantarum YDJ-03 as the main active ingredient.

[0014] Preferably, the amount of Lactobacillus plantarum YDJ-03 in the product is 1.0×10 6 -2.0×10 10 Between CFU.

[0015] Beneficial effects of the present invention: The present invention discovered for the first time a strain of Lactobacillus plantarum YDJ-03, which can effectively reduce the level of malondialdehyde in the liver of the body, enhance the activity of SOD in the liver of the body, reduce lipid peroxidation in the body, reduce the level of serum uric acid in the body, reduce the level of serum creatinine in the body, and relieve kidney damage. It can be used to prepare foods or medicines for preventing and treating kidney damage, protecting the liver, regulating fat, protecting the intestines, and anti-oxidation and anti-inflammatory, and has great application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 For liver MDA analysis; Figure 2 For liver SOD analysis; Figure 3 For serum uric acid analysis; Figure 4 For serum creatinine analysis; Figure 5 Analysis of the effect of Lactobacillus plantarum YDJ-03 on renal injury; (A) kidney HE staining; (B) kidney PAS staining; Figure 6 Analysis of the richness and diversity of intestinal bacteria in mice infected with Lactobacillus plantarum YDJ-03. DETAILED DESCRIPTION

[0017] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0018] Example 1: A strain of Lactobacillus plantarum YDJ-03, which was preserved at the General Microbiology Center of the China Culture Collection Administration on July 27, 2023, with the preservation address being the Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, with the preservation number being CGMCCNO: 28037, and the classification name being Lactobacillus plantarum.

[0019] Example 2: Application analysis of Lactobacillus plantarum YDJ-03: 1) Thirty SPF-grade C57BL / 6 male mice (18-22 g) were purchased from Shanghai Slake Laboratory Animal Co., Ltd. and divided into three groups, with 10 mice in each group, namely the normal group, the model group, and the Lactobacillus plantarum YDJ-03 group.

[0020] 2) Except for the normal group which was given a control diet (D09100304, ResearchDiets, USA), the other groups were fed a high-fat, high-fructose, high-cholesterol diet (D09100310, ResearchDiets, USA) supplemented with yeast powder and adenine for 90 days.

[0021] 3) Subsequently, the mice in the Lactobacillus plantarum YDJ-03 group were intragastrically administered with Lactobacillus plantarum YDJ-03 every day at a dose of 1.2×10 9 CFU count. The normal group and the model group were gavaged with pure water every day as a blank control. After 28 days of administration, except for the normal group, the mice in the other groups were intraperitoneally injected with hypoxanthine (400 mg / kg). After 1 hour, blood was collected from the inner canthus of the orbit. The blood was placed at room temperature for 2 hours and then centrifuged at 4°C to obtain serum. The uric acid and creatinine levels in the serum of the mice were determined by a veterinary automatic biochemical analyzer (BS-240VET, Shenzhen Mindray). The mice were killed, and the left lobe of the liver and the left kidney (longitudinal section) were taken, pathological sections were prepared, and hematoxylin-eosin (HE) staining was performed. The kidney sections were also stained with periodic acid Schiff (PAS). About 0.1 g of liver and kidney tissues were taken, and about 0.9 mL of physiological saline was homogenized to determine the glutaraldehyde (MDA) and superoxide dismutase (SOD) indicators. The cecal contents of the mice were taken, and 16srRNA sequencing (V3-V4 segment) was used to analyze the changes in the intestinal bacterial structure (n=6). 16S sequencing and analysis were performed by Shanghai Paisono Biotechnology Co., Ltd.

[0022] The results are as follows Figure 1-2 As shown in the figure, the MDA level in the model group increased significantly compared with the normal group, while the administration of Lactobacillus plantarum YDJ-03 significantly reduced the MDA level in the liver. Compared with the model group, the administration of Lactobacillus plantarum YDJ-03 increased the activity of liver SOD. The above results can be concluded that the liver protective effect of Lactobacillus plantarum YDJ-03 is related to the reduction of lipid peroxidation. Figure 3-4 As shown in the figure, compared with the normal group, the serum uric acid and creatinine levels in the model group increased significantly, and the administration of Lactobacillus plantarum YDJ-03 significantly reduced the uric acid and creatinine levels. Figure 5 As shown in Figure 1, the renal cells of mice in the normal group were arranged tightly and orderly, the renal tubules were clearly outlined, and the cells were not swollen; the renal tubular epithelial cells of mice in the model group were severely swollen, accompanied by a large number of inflammatory cell infiltrations; the renal tubular epithelial cells of mice in the Lactobacillus plantarum YDJ-03 group were slightly to moderately swollen, and the inflammatory cell infiltration was reduced. PAS staining ( Figure 5 B) shows that compared with the kidneys of the normal group of mice, the glomerular basement membrane of the model group mice was thickened, the mesangial cells proliferated, and the extracellular matrix increased. The administration of Lactobacillus plantarum YDJ-03 significantly improved the above pathological conditions. Figure 6 As shown in the figure, the Chao1 index is used to characterize species richness, and the Simpson and Shannon indices are used to characterize species diversity. The sequencing results show that Lactobacillus plantarum YDJ-03 has a tendency to increase the richness of intestinal bacteria, but there is no significant change in intestinal bacteria diversity. In summary, Lactobacillus plantarum YDJ-03 can significantly reduce uric acid and creatinine levels and alleviate kidney damage.

[0023] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A strain of Lactobacillus plantarum YDJ-03, characterized in that: It was deposited at the General Microbiology Center of the China Culture Collection Administration on July 27, 2023. The storage address is the Institute of Microbiology, Chinese Academy of Sciences, No. 3, Building 1, Beichen West Road, Chaoyang District, Beijing. The storage number is CGMCCNO: 28037, and the classification name is Lactobacillus plantarum.

2. Use of the plant lactobacillus YDJ-03 described in claim 1 in the preparation of products for preventing and treating kidney damage, protecting the liver, regulating fat, protecting the intestines, and anti-oxidation and anti-inflammatory.

3. The use as claimed in claim 2, characterized in that The application is that Lactobacillus plantarum YDJ-03 can effectively reduce the level of malondialdehyde in the liver of the body.

4. The use as claimed in claim 2, characterized in that The application is that Lactobacillus plantarum YDJ-03 can effectively enhance the SOD activity of the liver.

5. The use as claimed in claim 2, characterized in that The application is that Lactobacillus plantarum YDJ-03 can effectively reduce lipid peroxidation in the body.

6. The use as claimed in claim 2, characterized in that The application is that Lactobacillus plantarum YDJ-03 can effectively reduce the body's serum uric acid level.

7. The use as claimed in claim 2, characterized in that The application is that Lactobacillus plantarum YDJ-03 can effectively reduce the body's serum creatinine level.

8. The use as claimed in claim 2, characterized in that The application is that Lactobacillus plantarum YDJ-03 can effectively alleviate kidney damage.

9. A product for preventing and treating kidney damage, protecting the liver, regulating fat, protecting the intestines, anti-oxidation and anti-inflammatory, characterized in that: The product uses the plant lactobacillus YDJ-03 described in claim 1 as the main active ingredient.

10. The product according to claim 9, characterized in that The amount of Lactobacillus plantarum YDJ-03 in the product is 1.0×10 6 -2.0×10 10 Between CFU.

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