Stenotrophomonas maltophilia 6: 2FTS-SX001 as well as culture method and application thereof

Abstract

The invention provides stenotrophomonas maltophilia 6: 2FTS-SX001 as well as a culture method and application thereof, and particularly belongs to the technical field of microorganisms. The stenotrophomonas maltophilia 6: 2FTS-SX001 provided by the invention is preserved in the General Microbiological Culture Collection Center of the China Committee for Culture Collection of Microorganisms, and the preservation number of the stenotrophomonas maltophilia 6: 2FTS-SX001 is CGMCC No.32170. The stenotrophomonas maltophilia 6: 2FTS-SX001 provided by the invention has the advantages that the stenotrophomonas maltophilia The stenotrophomonas maltophilia 6: 2FTS-SX001 can grow in a culture medium taking 6: 2 fluorine telomeric sulfonate as a unique sulfur source, defluorination degradation of the 6: 2 fluorine telomeric sulfonate can be realized, and pollution of the 6: 2 fluorine telomeric sulfonate and conversion products thereof in soil, surface water and / or underground water can be effectively treated; and the method is beneficial for promoting the restoration of perfluorinated and polyfluoroalkyl substance pollution.

Description

Technical Field

[0001] The invention belongs to the technical field of microorganisms, and specifically relates to a strain of Stenotrophomonas maltophilia 6:2FTS-SX001 and a culture method and application thereof. Background Art

[0002] Per- and polyfluoroalkyl substances (PFASs) are a class of synthetic fluorinated organic compounds with hydrophobic, oleophobic, thermally stable and surface-active properties, and are widely used in industry and daily life. Studies have shown that PFASs exhibit persistence, long-range mobility, bioaccumulation and biotoxicity in the environment, causing serious impacts on the ecological environment and human health, and have become a new global pollutant. 6:2 Fluorotelomer Sulfonate (6:2FTS) is a new PFASs substitute and has been frequently detected in various environmental media in recent years. However, there are few studies on the microbial degradation of 6:2FTS at home and abroad, and there are few reported bacterial communities and strain resources. Therefore, the acquisition of 6:2FTS defluorination-degrading bacteria is of great practical significance for promoting the research and development of PFASs microbial remediation technology. Summary of the invention

[0003] The object of the present invention is to provide a strain of Stenotrophomonas maltophilia 6:2FTS-SX001 and a culture method and application thereof. The Stenotrophomonas maltophilia 6:2FTS-SX001 of the present invention can achieve defluorination degradation of 6:2 fluorotelomer sulfonate, which is beneficial to promote the remediation of perfluoro and polyfluoroalkyl substance pollution.

[0004] The invention provides a strain of Stenotrophomonas maltophilia 6:2FTS-SX001. The Stenotrophomonas maltophilia 6:2FTS-SX001 is deposited in the General Microbiological Center of China National Committee for Microbiological Culture Collection, with a deposit number of CGMCC No.32170.

[0005] The present invention also provides a method for culturing the Stenotrophomonas maltophilia 6:2FTS-SX001 described in the above technical solution, comprising the following steps: inoculating the Stenotrophomonas maltophilia 6:2FTS-SX001 into a culture medium for fermentation culture to obtain the Stenotrophomonas maltophilia 6:2FTS-SX001.

[0006] Preferably, the culture temperature is 25-35°C.

[0007] Preferably, the culture medium comprises a sulfur-free culture medium and a sulfur source; and the sulfur source comprises 6:2 fluorotelomer sulfonate.

[0008] Preferably, the sulfur-free culture medium comprises: CH3COONa 5.0 g, NH4Cl 1.55 g, KH2PO4 0.4 g, K2HPO4 1.6 g, MgCl2 0.165 g, NaCl 2.0 g, CaCl2·2H2O 0.09 g, trace element solution 0.1 mL, Wolin vitamin solution 0.1 mL and distilled water 1000 mL, pH 7.0.

[0009] Preferably, the trace element solution comprises a trace element solution A and a trace element solution B; the trace element solution A comprises 10 mL / L of 25% HCl aqueous solution, 1.5 g / L of FeCl2·4H2O, 0.19 g / L of CoCl2·6H2O, 0.1 g / L of MnCl2·4H2O, 70 mg / L of ZnCl2, 6 mg / L of H3BO, 36 mg / L of Na2MoO4·2H2O, 24 mg / L of NiCl2·6H2O and 2 mg / L of CuCl2·2H2O; the trace element solution B comprises 6 mg / L of Na2SeO3·5H2O, 8 mg / L of Na2WO4·2H2O and 0.5 g / L of NaOH; the solvent is water;

[0010] Preferably, the Wolin vitamin solution comprises 20 mg biotin, 20 mg folic acid, 100 mg pyridoxine hydrochloride, 50 mg riboflavin, 50 mg vitamin B1, 50 mg niacin, 50 mg pantothenic acid, 1 mg vitamin B12, 50 mg p-aminobenzoic acid, 50 mg lipoic acid and 100 mL water.

[0011] The present invention also provides a fermentation culture of Stenotrophomonas maltophilia 6:2FTS-SX001 obtained based on the culturing method described in the above technical solution, wherein the types of the fermentation culture include bacterial cells and / or extracellular metabolic active substances.

[0012] The present invention also provides the use of the Stenotrophomonas maltophilia 6:2FTS-SX001 described in the above technical scheme or the Stenotrophomonas maltophilia 6:2FTS-SX001 obtained by the cultivation method described in the above technical scheme in degrading 6:2 fluoropolymer sulfonate and its conversion products; the conversion products include 5:3 fluoropolymer carboxylic acid, 5:3 fluoropolymer unsaturated carboxylic acid, 6:2 fluoropolymer carboxylic acid, 6:2 fluoropolymer unsaturated carboxylic acid, 6:2 fluoropolymer alcohol, 6:2 fluoropolymer aldehyde and 4:2 fluoropolymer carboxylic acid.

[0013] The present invention also provides the use of the Stenotrophomonas maltophilia 6:2FTS-SX001 described in the above technical scheme or the Stenotrophomonas maltophilia 6:2FTS-SX001 obtained by the cultivation method described in the above technical scheme in preventing and controlling pollution by 6:2 fluorotelomer sulfonate and its transformation products; the pollution includes pollution of any one or more of soil, surface water and groundwater; the transformation products include 5:3 fluorotelomer carboxylic acid, 5:3 fluorotelomer unsaturated carboxylic acid, 6:2 fluorotelomer carboxylic acid, 6:2 fluorotelomer unsaturated carboxylic acid, 6:2 fluorotelomer alcohol, 6:2 fluorotelomer aldehyde and 4:2 fluorotelomer carboxylic acid.

[0014] The present invention provides a strain of Stenotrophomonas maltophilia 6:2FTS-SX001. The Stenotrophomonas maltophilia 6:2FTS-SX001 of the present invention can grow in a culture medium with 6:2 fluorotelomer sulfonate as the only sulfur source, can achieve defluorination and degradation of 6:2 fluorotelomer sulfonate, effectively control the pollution of 6:2 fluorotelomer sulfonate in soil, surface water and / or groundwater, and is conducive to promoting the remediation of perfluoro and polyfluoroalkyl substance pollution. The test results show that within the 7-day culture period of the Stenotrophomonas maltophilia 6:2FTS-SX001 of the present invention, the concentration of 6:2 fluorotelomer sulfonate decreases rapidly and eventually falls below the detection line. The Stenotrophomonas maltophilia 6:2FTS-SX001 of the present invention can achieve rapid and complete degradation of 6:2 fluorotelomer sulfonate. Under sulfur-restricted conditions, the release of fluoride ions by 6:2FTS-SX001 was measured by ISE. On the third day, the fluoride ion generation was observed to be 42±0.93μg / L, and the maximum defluorination (138.66±1.29μg / L) was achieved on the 21st day of culture, indicating that the Stenotrophomonas maltophilia 6:2FTS-SX001 can achieve rapid and complete degradation of 6:2 fluorotelomer sulfonate. The CF bond in 6:2 fluoropolysulfonate was broken to release fluoride ions; under sulfur-limited conditions, the growth of 6:2FTS-SX001 gradually increased during the 7-day culture period, but then began to decline. Combined with the fact that 6:2 fluoropolysulfonate was completely degraded on the 7th day, 5:3 fluoropolycarboxylic acid, 5:3 fluoropolyunsaturated carboxylic acid, 6:2 fluoropolycarboxylic acid, 6:2 fluoropolyunsaturated carboxylic acid, 6:2 fluoropolyol, 6:2 fluoropolyaldehyde and 4:2 fluoropolycarboxylic acid were detected in the conversion products, indicating that 6:2FTS-SX001 can utilize the sulfur in the sulfonic acid group in 6:2FTS to support its growth and energy needs. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0016] Figure 1 The colony morphology diagram provided by the present invention;

[0017] Figure 2 A sequence alignment result diagram provided by the present invention;

[0018] Figure 3 This is a graph showing the results of a degradation experiment of 6:2FTS by the strain provided by the present invention;

[0019] Figure 4 This is a graph showing the release of fluoride ions during the degradation of 6:2FTS by the strain provided by the present invention;

[0020] Figure 5 This is a growth curve diagram of the strain provided by the present invention during the process of degrading 6:2FTS.

[0021] Biological deposit information

[0022] Stenotrophomonas maltophilia 6:2FTS-SX001 is deposited in the General Microbiological Center of China Microbiological Culture Collection Administration, referred to as CGMCC, and the deposit date is October 10, 2024. The address is No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing, and the deposit number is CGMCC No. 32170. DETAILED DESCRIPTION

[0023] The present invention provides a strain of Stenotrophomonas maltophilia 6:2FTS-SX001, wherein the Stenotrophomonas maltophilia 6:2FTS-SX001 is deposited in the General Microbiological Center of China Microbiological Culture Collection Administration Committee, and the deposit number is CGMCC No. 32170. The colony morphology of the Stenotrophomonas maltophilia 6:2FTS-SX001 on the LB plate is light yellow and millet-like, and the similarity with the strain Stenotrophomonas maltophilia is 99.64% through 16S rRNA gene sequence comparison analysis. The Oligotrophomonas maltophilia 6:2FTS-SX001 described in the present invention is a 6:2 fluorotelomer sulfonate defluorination and degradation bacterium, which can grow in a culture medium with 6:2 fluorotelomer sulfonate as the only sulfur source, and can achieve defluorination and degradation of 6:2 fluorotelomer sulfonate and its transformation products, effectively control the pollution of 6:2 fluorotelomer sulfonate in soil, surface water and / or groundwater, and is conducive to promoting the remediation of perfluorinated and polyfluoroalkyl substance pollution.

[0024] The present invention also provides a method for culturing the Stenotrophomonas maltophilia 6:2FTS-SX001 described in the above technical solution, comprising the following steps: inoculating the Stenotrophomonas maltophilia 6:2FTS-SX001 into a culture medium for fermentation culture to obtain the Stenotrophomonas maltophilia 6:2FTS-SX001.

[0025] In a specific embodiment, the culture temperature may be 25-35°C, specifically 30°C or 32°C. In a specific embodiment, the fermentation culture may be a shaking culture, and the speed may be 150-200rpm, specifically 160rpm. In a specific embodiment, the culture medium may include a sulfur-free culture medium and a sulfur source. In a specific embodiment, the sulfur source is 6:2 fluorotelomer sulfonate.

[0026] In a specific embodiment, the sulfur source includes 6:2 fluorotelomer sulfonate. In a specific embodiment, the sulfur-free culture medium includes: CH3COONa 5.0g, NH4Cl 1.55g, KH2PO4 0.4g, K2HPO4 1.6g, MgCl2 0.165g, NaCl 2.0g, CaCl2·2H2O 0.09g, trace element solution 0.1mL, Wolin vitamin solution 0.1mL and distilled water 1000mL, pH 7.0. In a specific embodiment, the trace element solution includes trace element solution A and trace element solution B; the trace element solution A includes 10 mL / L of HCl aqueous solution with a mass percentage of 25%, FeCl2·4H2O 1.5 g / L, CoCl2·6H2O 0.19 g / L, MnCl2·4H2O 0.1 g / L, ZnCl2 70 mg / L, H3BO 6 mg / L, Na2MoO4·2H2O 36 mg / L, NiCl2·6H2O 24 mg / L and CuCl2·2H2O 2 mg / L; the trace element solution B includes Na2SeO3·5H2O 6 mg / L, Na2WO4·2H2O 8 mg / L and NaOH 0.5 g / L; the solvent is water. In a specific embodiment, the Wolin vitamin solution includes 20 mg biotin, 20 mg folic acid, 100 mg pyridoxine hydrochloride, 50 mg riboflavin, 50 mg vitamin B1, 50 mg niacin, 50 mg pantothenic acid, 1 mg vitamin B12, 50 mg p-aminobenzoic acid, 50 mg lipoic acid and 100 mL water.

[0027] The present invention also provides a fermentation culture of Stenotrophomonas maltophilia 6:2FTS-SX001 obtained by the culturing method described in the above technical solution, wherein the type of the fermentation culture includes bacterial cells and / or extracellular metabolic active substances. The present invention has no particular limitation on the type of the fermentation culture, and it can be any form of product obtained by fermenting the Stenotrophomonas maltophilia 6:2FTS-SX001 of the present invention.

[0028] The present invention also provides the use of the Stenotrophomonas maltophilia 6:2FTS-SX001 described in the above technical scheme or the Stenotrophomonas maltophilia 6:2FTS-SX001 obtained by the cultivation method described in the above technical scheme in degrading 6:2 fluoropolymer sulfonate and its conversion products; the conversion products include 5:3 fluoropolymer carboxylic acid, 5:3 fluoropolymer unsaturated carboxylic acid, 6:2 fluoropolymer carboxylic acid, 6:2 fluoropolymer unsaturated carboxylic acid, 6:2 fluoropolymer alcohol, 6:2 fluoropolymer aldehyde and 4:2 fluoropolymer carboxylic acid.

[0029] The present invention also provides the use of the Stenotrophomonas maltophilia 6:2FTS-SX001 described in the above technical scheme or the Stenotrophomonas maltophilia 6:2FTS-SX001 obtained by the cultivation method described in the above technical scheme in preventing and controlling pollution by 6:2 fluorotelomer sulfonate and its transformation products; the pollution includes pollution of any one or more of soil, surface water and groundwater; the transformation products include 5:3 fluorotelomer carboxylic acid, 5:3 fluorotelomer unsaturated carboxylic acid, 6:2 fluorotelomer carboxylic acid, 6:2 fluorotelomer unsaturated carboxylic acid, 6:2 fluorotelomer alcohol, 6:2 fluorotelomer aldehyde and 4:2 fluorotelomer carboxylic acid.

[0030] In order to further illustrate the present invention, a strain of Stenotrophomonas maltophilia 6:2FTS-SX001 and its culture method and application provided by the present invention are described in detail below in conjunction with the drawings and examples, but they should not be construed as limiting the scope of protection of the present invention.

[0031] Sulfur-free culture medium: CH3COONa 5.0g, NH4Cl 1.55g, KH2PO4 0.4 g, K2HPO4 1.6 g, MgCl2 0.165g, NaCl, 2.0g, CaCl2·2H2O 0.09g, trace element solution 0.1mL, Wolin vitamin solution 0.1mL, distilled water 1000mL, pH 7.0.

[0032] The specific formula of trace elements and Wolin vitamin solution is as follows:

[0033] Trace element solution: Trace element solution A: HCl (25% solution, w / w) 10 mL / L, FeCl2·4H2O 1.5 g / L, CoCl2·6H2O 0.19 g / L, MnCl2·4H2O 0.1 g / L, ZnCl2 70 mg, H3BO 6 mg / L, Na2MoO4·2H2O 36 mg / L, NiCl2·6H2O 24 mg / L, CuCl2·2H2O 2 mg / L; Trace element solution B: Na2SeO3·5H2O 6 mg / L, Na2WO4·2H2O 8 mg / L, NaOH 0.5 g / L; the solvent is water.

[0034] Wolin Vitamin Solution: 20 mg biotin, 20 mg folic acid, 100 mg pyridoxine hydrochloride, 50 mg riboflavin, 50 mg vitamin B1, 50 mg niacin, 50 mg pantothenic acid, 1 mg vitamin B12, 50 mg p-aminobenzoic acid, 50 mg lipoic acid dissolved in 100 mL of sterile ultrapure water.

[0035] LB medium: 5.0 g beef extract, 10.0 g peptone, 3.0 g NaCl, 15 g agar powder, 1000 mL distilled water, pH 7.0.

[0036] Example 1

[0037] 1. Isolation and identification of strains

[0038] Experimental methods:

[0039] 1) Soil samples were collected from a retired chemical plant in Zhangjiagang City, Jiangsu Province, which was contaminated by chlorinated hydrocarbons and PFASs. Soil was collected from a depth of 0.5m underground, placed in a sterile sampling bag, and stored in a 4°C cold storage. The collected soil was air-dried, ground, and sieved through a 20-mesh sieve to remove stones and plant matter.

[0040] 2) Prepare sulfur-free culture medium and sterilize it at 121℃ for 20 minutes. To prevent the trace element and vitamin solutions from becoming ineffective, only the inorganic salt components are sterilized at high temperature and high pressure during the sterilization process. After the culture medium is cooled to room temperature, the trace element solutions A and B and the Wolin vitamin solution are filtered and sterilized using a 0.22μm sterile microporous filter membrane. The above operations are mixed and completed in an ultra-clean workbench.

[0041] 3) Weigh 5 g of the sieved soil and add it to a 100 mL conical flask containing 50 mL of sulfur-free culture medium, add 1 mg / L 6:2FTS as the only sulfur source, place it in a shaker, and culture it at 30°C and 160 rpm for 7 days.

[0042] 4) After 7 days of culture, 2% of the acclimation solution was inoculated into a new sulfur-free medium, and 6:2FTS was added to the medium to a final concentration of 3 mg / L. The culture conditions were not changed and the shaking culture was continued for 7 days. The initial culture concentrations of 6:2FTS were 5 mg / L, 10 mg / L, 25 mg / L and 50 mg / L, respectively. The OD was measured. 600 Monitor the growth of microbial communities and analyze the changes in the concentrations of 6:2FTS and the final product - fluoride ions to determine whether defluorination degradation has been achieved.

[0043] 5) The above-mentioned microbial domestication community that defluorinated 6:2FTS was spread on LB solid culture medium, and single colonies showing different morphological characteristics on agar were selected, and pure cultures were obtained by 3-4 times of streaking purification. These pure cultures derived from single colonies were tested for 6:2FTS degradation ability in sulfur-free medium, and finally a pure culture strain that can achieve rapid defluorination degradation of 6:2FTS was screened out.

[0044] The 16S rRNA gene sequence of the strain:

[0045] GCGTCATCCCGATGGTTATGCTACCTGCTTCTGGTGCAACAAACTCCCATGGTGTGACGGGCGGTGTGTACAAGGCCCGGGAACGTATTCACCGCAGCAATGCTGATCTGCGATTACTAGCGATTCCGACTTCATGGAGTCGAGTTGCAGACTCCAATCCGGACTGAGATAGGGTTTCTGGGATTGGCTTACCGTCGCCGGCTTGCAGCCCTCTGTCCCTACCATTGTAGTACGTGTGTAGCCCTGGCCGTAAGGGCCATGATGACTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCGGTCTCCTTAGAGTTCCCACCATTACGTGCTGGCAACTAAGGACAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACGACACGAGCTGACGACAGCCATGCAGCACCTGTGTTCGAGTTCCCGAAGGCACCAATCCATCTCTGGAAAGTTCTCGACATGTCAAGGCCAGGTAAGGTTCTTCGCGTTGCATCGAATTAAACCACATACTCCACCGCTTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCAGTCTTGCGACCGTACTCCCCAGGCGGCGAACTTAACGCGTTAGCTTCGATACTGCGTGCCAAATTGCACCCAACATCCAGTTCGCATCGTTTAGGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTGTTGGTCCAGGTAGCTGCCTTCGCCATGAATGTTCCTCCTGATCTCTACGCATTTCACTGCTACACCACGAATTCCG(SEQ ID NO.1).

[0046] The colony morphology of the screened 6:2FTS defluorination-degrading strain on the LB plate was light yellow and millet-like ( Figure 1 ); The 16S rRNA gene sequence analysis showed that the strain had a similarity of 99.64% with the strain Stenotrophomonas maltophilia ( Figure 2 ). According to the above colony morphology and 16S rRNA gene sequence comparison results, the strain was finally identified and named Stenotrophomonas maltophilia 6:2FTS-SX001, and was deposited in the General Microbiological Center of China Microbiological Culture Collection Administration with the deposit number CGMCC No.32170.

[0047] 2. Experiment on the degradation of 6:2FTS by strains

[0048] 2.1 Degradation effect of strains on 6:2FTS

[0049] An inoculum of 6:2 FTS-SX001 enriched in LB medium was used. The cell suspension was pelleted by centrifugation at 10,000 g for 20 min, and the cell pellet was washed 2 to 3 times with phosphate buffered saline (PBS), then resuspended in PBS and its OD 600 is 1.0±0.1 for subsequent inoculation. The present invention studies the biodegradation ability of maltophilia 6:2FTS-SX001 on 6:2FTS under sulfur-restricted conditions. Specifically, the experiment was carried out aerobically in a 100mL conical flask containing 50mL of sulfur-free culture medium. 6:2FTS-SX001 with 6:2FTS (8mg / L) as the only sulfur source was inoculated as the biodegradation group, and the corresponding sterile control group was prepared in the same manner, containing 6:2FTS, but not inoculated with the bacteria. All treatments were repeated three times and cultured at a constant temperature of 30°C and 160rpm in a shaker for 30 days. On the 0th, 3rd, 7th, 15th, 21st and 30th days of culture, 2.0mL of samples were taken, centrifuged, filtered, and the degradation and defluorination of 6:2FTS were detected, and the growth of the strain during the degradation process was monitored.

[0050] The degradation of 6:2FTS was monitored using a Waters ACQUITY ultra-high performance liquid chromatography system (UPLC-MS / MS) (Waters Corporation, Milford, MA, USA) equipped with a Waters Xevo TQ-S Micro triple quadrupole mass spectrometer. The instrument was operated in multiple reaction monitoring mode (MRM) using negative electrospray ionization (ESI). Target and non-target intermediates were identified by UPLC-MS / MS and liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QTOF-MS, X500R, Sciex, Framingham, MA, USA). A BEH C18 analytical column (100 mm×3.0 mm, 1.7 μm, Waters, MA, USA) was used to separate 3 μL of the injected diluted sample.

[0051] Under sulfur-limited conditions, the degradation of 6:2FTS concentration over time by 6:2FTS-SX001 was analyzed by LC-MS / MS. Figure 3As shown, the results showed that within the 7-day culture period, the concentration of 6:2FTS decreased rapidly and eventually fell below the detection line, indicating that the strain achieved rapid and complete degradation of 6:2FTS. In addition, seven biotransformation products were identified and quantified from the 6:2FTS biodegradation group, namely 5:3 fluorotelomer carboxylic acid, 5:3 fluorotelomer unsaturated carboxylic acid, 6:2 fluorotelomer carboxylic acid, 6:2 fluorotelomer unsaturated carboxylic acid, 6:2 fluorotelomer alcohol, 6:2 fluorotelomer aldehyde and 4:2 fluorotelomer carboxylic acid.

[0052] 2.2 Fluoride ion release during the degradation of 6:2FTS by strains

[0053] The concentration of fluoride ions was measured by a fluoride ion selective electrode (ISE, Sanxin). Before each measurement, the ISE was calibrated according to the manufacturer's guidelines. In the sample measurement, the sample was diluted to the same volume as TISAB buffer (total ionic strength adjustment buffer) to keep the ionic strength constant during the measurement. Among them, the detection limit of fluoride ions is 25μg / L. Before measurement, 2mL of TISAB was added to 2mL of sample and shaken thoroughly before testing.

[0054] Under sulfur-limited conditions, the release of fluoride ions from 6:2FTS-SX001 was measured by ISE. Figure 4 As shown, the fluoride ion production was observed to be 42±0.93 μg / L on the 3rd day, and the maximum defluorination (138.66±1.29 μg / L) was achieved on the 21st day of cultivation. These results indicate that Stenotrophomonas maltophilia 6:2FTS-SX001 releases fluoride ions by breaking the CF bond in 6:2FTS.

[0055] 2.3 Growth curve of strain during degradation of 6:2FTS

[0056] The optical density (OD) was measured at a wavelength of 600 nm. 600 ) Monitor the growth of microorganisms on days 0, 3, 7, 15, 21 and 30 of culture.

[0057] In degradation experiments, OD 600 Monitoring the growth of Stenotrophomonas maltophilia 6:2FTS-SX001 under sulfur-limited conditions. Figure 5 As shown, in the presence of 6:2FTS, the growth of Stenotrophomonas maltophilia 6:2FTS-SX001 was observed to gradually increase over the 7-day culture period but then began to decline, with complete degradation of the bound 6:2FTS achieved on the 7th day, indicating that the strain can utilize the sulfur in the sulfonic acid group of 6:2FTS to support its growth and energy requirements.

[0058] in conclusion:

[0059] Stenotrophomonas maltophilia 6:2FTS-SX001 can achieve rapid degradation of 6:2FTS, while releasing fluoride ions and breaking the CF bond.

[0060] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A strain of Stenotrophomonas maltophilia 6:2FTS-SX001, characterized in that: The Stenotrophomonas maltophilia 6:2FTS-SX001 is deposited in the General Microbiological Center of China Culture Collection Administration, with the deposit number of CGMCC No.32170.

2. The method for culturing the Stenotrophomonas maltophilia 6:2FTS-SX001 according to claim 1, comprising the following steps: The Stenotrophomonas maltophilia 6:2FTS-SX001 was inoculated into a culture medium for fermentation culture to obtain a fermentation culture of the Stenotrophomonas maltophilia 6:2FTS-SX001.

3. The culture method according to claim 2, characterized in that The culture temperature is 25-35°C.

4. The culture method according to claim 2, characterized in that The culture medium comprises a sulfur-free culture medium and a sulfur source; the sulfur source comprises 6:2 fluorotelomer sulfonate.

5. The culture method according to claim 4, characterized in that The sulfur-free culture medium comprises: CH3COONa 5.0 g, NH4Cl 1.55 g, KH2PO4 0.4 g, K2HPO4 1.6 g, MgCl2 0.165 g, NaCl 2.0 g, CaCl2·2H2O 0.09 g, trace element solution 0.1 mL, Wolin vitamin solution 0.1 mL and distilled water 1000 mL, pH 7.

0.

6. The culture method according to claim 5, characterized in that: The trace element solution comprises a trace element solution A and a trace element solution B; the trace element solution A comprises 10 mL / L of HCl aqueous solution with a mass percentage of 25%, 1.5 g / L of FeCl2·4H2O, 0.19 g / L of CoCl2·6H2O, 0.1 g / L of MnCl2·4H2O, 70 mg / L of ZnCl2, 6 mg / L of H3BO, 36 mg / L of Na2MoO4·2H2O, 24 mg / L of NiCl2·6H2O and 2 mg / L of CuCl2·2H2O; the trace element solution B comprises 6 mg / L of Na2SeO3·5H2O, 8 mg / L of Na2WO4·2H2O and 0.5 g / L of NaOH; and the solvent is water.

7. The culture method according to claim 5, characterized in that The Wolin vitamin solution includes 20 mg of biotin, 20 mg of folic acid, 100 mg of pyridoxine hydrochloride, 50 mg of riboflavin, 50 mg of vitamin B1, 50 mg of niacin, 50 mg of pantothenic acid, 1 mg of vitamin B12, 50 mg of p-aminobenzoic acid, 50 mg of lipoic acid and 100 mL of water.

8. A fermentation culture of Stenotrophomonas maltophilia 6:2FTS-SX001 obtained by the culture method according to any one of claims 2 to 7, characterized in that: The types of the fermentation culture include bacterial cells and / or extracellular metabolically active substances.

9. Use of the fermentation culture of the Stenotrophomonas maltophilia 6:2FTS-SX001 according to claim 1 or the Stenotrophomonas maltophilia 6:2FTS-SX001 obtained by the culturing method according to any one of claims 2 to 7 in the degradation of 6:2 fluorotelomer sulfonate and its conversion products; the conversion products include 5:3 fluorotelomer carboxylic acid, 5:3 fluorotelomer unsaturated carboxylic acid, 6:2 fluorotelomer carboxylic acid, 6:2 fluorotelomer unsaturated carboxylic acid, 6:2 fluorotelomer alcohol, 6:2 fluorotelomer aldehyde and 4:2 fluorotelomer carboxylic acid.

10. Use of the fermentation culture of the Stenotrophomonas maltophilia 6:2FTS-SX001 according to claim 1 or the Stenotrophomonas maltophilia 6:2FTS-SX001 obtained by the culturing method according to any one of claims 2 to 7 in preventing and controlling pollution by 6:2 fluorotelomer sulfonate and its conversion products; the pollution comprises any one or more of soil, surface water and groundwater pollution; the conversion products comprise 5:3 fluorotelomer carboxylic acid, 5:3 fluorotelomer unsaturated carboxylic acid, 6:2 fluorotelomer carboxylic acid, 6:2 fluorotelomer unsaturated carboxylic acid, 6:2 fluorotelomer alcohol, 6:2 fluorotelomer aldehyde and 4:2 fluorotelomer carboxylic acid.

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