Diaphorina citri adult repellent composition and application thereof

Abstract

The invention discloses a diaphorina citri adult repellent composition and application thereof. The repellent composition is prepared from the following components in parts by weight: 0.1 to 1.2 parts of nootkatone, 0.05 to 1.0 part of carvacrol, 4.0 to 6.5 parts of thymol and 3.0 to 5.0 parts of sophocarpidine. The repelling effect of the repellent composition on diaphorina citri adults is better than that of a single component, and the synergistic diaphorina citri adults repelling effect is achieved among the components. The components of the repellent composition are low in price, the cost for manufacturing the lure can be reduced, and the repellent composition is suitable for popularization and promotion.

Description

Technical Field

[0001] The invention belongs to the technical field of pest control, and particularly relates to a repellent composition for adult citrus psyllids and application thereof. Background Art

[0002] Citrus psyllid is the only insect vector that spreads citrus Huanglongbing. Controlling the occurrence and spread of this pest can prevent the spread of Huanglongbing and solve this key problem that restricts the modernization of the citrus industry. At present, the prevention and control of citrus psyllid at home and abroad basically relies on chemical pesticides. However, the large-scale, multiple, continuous and unreasonable long-term use of traditional chemical pesticides not only increases the cost of prevention and control, damages the ecological environment, kills natural enemies, increases pesticide residues in fruits, but also causes citrus psyllid to develop a certain degree of drug resistance. Therefore, finding a new method that can effectively, safely and sustainably control the spread of Huanglongbing by citrus psyllid is an urgent problem to be solved in the industrialization of citrus in my country.

[0003] Plant-derived repellents are a new type of agent that is derived from nature, biodegradable, and environmentally friendly. They have now become the focus of research and development. If we can identify plant-derived compounds that repel citrus psyllid adults and prevent citrus psyllid adults from feeding and laying eggs, we can prevent citrus psyllids from spreading the citrus Huanglongbing pathogen. Summary of the invention

[0004] The purpose of the present invention is to overcome the problems that existing citrus psyllid repellent compounds cannot effectively repel citrus psyllid adult insects from feeding and laying eggs, and the cost of chemical drugs is high, and to provide a repellent composition for citrus psyllid adult insects suitable for actual production and its application, so as to improve the repellent activity and reduce the field production cost.

[0005] The first object of the present invention is to provide a repellent composition for citrus psyllid adults, which comprises the following components in parts by weight: 0.1 to 1.2 parts of nootkatone, 0.05 to 1.0 parts of carvacrol, 4.0 to 6.5 parts of thymol, and 3.0 to 5.0 parts of matrine.

[0006] Preferably, the repellent composition comprises the following components in parts by weight: 0.5-0.8 parts of nootkatone, 0.1-0.8 parts of carvacrol, 4.5-6.0 parts of thymol, and 3.5-4.5 parts of matrine.

[0007] Further preferably, the repellent composition comprises the following components in parts by weight: 0.6 parts of nootkatone, 0.3 parts of carvacrol, 5.1 parts of thymol, and 4.0 parts of matrine.

[0008] The second object of the present invention is to provide the use of the above repellent composition in repelling and controlling citrus psyllids.

[0009] Preferably, the application includes applying the repellent composition to citrus leaves to directly repel citrus psyllid adults from sucking and laying eggs; or using the repellent composition in combination with an agricultural nano slow-release agent.

[0010] The third object of the present invention is to provide a product for repelling and controlling citrus psyllids, which comprises the repellent composition for citrus psyllid adults.

[0011] Preferably, the dosage form of the product includes emulsion, powder, aqueous solution, ointment, tincture, cream, aerosol or tablet.

[0012] The fourth object of the present invention is to provide a method for controlling and / or monitoring adult citrus psyllids, which comprises applying the above-mentioned repellent composition or product to the habitat of citrus psyllids to repel citrus psyllids.

[0013] Preferably, the dosage of the repellent composition is 0.5 to 10 mg / m2.

[0014] Compared with the prior art, the advantages of the present invention are:

[0015] (1) Compared with traditional chemical pesticides, the present invention is a plant-derived repellent compound that does not cause problems such as drug resistance and environmental pollution.

[0016] (2) Compared with general plant-derived repellent compounds, the active ingredients of the citrus psyllid repellent composition of the present invention have stronger persistence in the field, which can significantly reduce the manpower required for monitoring / control.

[0017] (3) The repellent composition provided by the present invention has a better repellent effect on adult citrus psyllids than the use of a single component, and the components exert a synergistic repellent effect on adult citrus psyllids. In addition, the components of the repellent composition are low in price, which can reduce the cost of making the repellent, and is suitable for popularization and promotion. DETAILED DESCRIPTION

[0018] The present invention is further described in detail below in conjunction with specific embodiments. The given examples are only for illustrating the present invention, but not for limiting the scope of the present invention.

[0019] Example 1

[0020] A behavioral test of citrus psyllid repellent was conducted at Zhongkai University of Agriculture and Engineering from July 1 to 15, 2023. Nootkatone, carvacrol, thymol, and matrine were prepared with n-hexane as solvent at a concentration of 10 mg / ml. The four substances were mixed according to the components in Table 1 to prepare a repellent mixed solution with a total concentration of 10 mg / ml. 10 μl of the solution was tested.

[0021] The fruit fly culture tube was evenly divided into 3 chambers, of which the one farthest from the odor source was the recording position. Then, after collecting the citrus psyllid adults and starving them for 2 hours, 10 psyllid adults were placed in the fruit fly tube, the tube mouth was blocked with absorbent cotton, and the psyllid adults were allowed to recover for 10 minutes to move freely in the tube. Then, a cotton swab was used to absorb the candidate repellent compound (see Table 1), inserted into the tube mouth, and the number of citrus psyllids far away from the odor source was recorded, counted at intervals of 5 seconds, and the average measurement result of 10 consecutive times was recorded as the escape index. This experiment recorded 10 biological replicates of male and female insects, and the number of insects was measured for 10 in each biological replicate.

[0022] In the test data of Table 2, groups AF to DM are the repellent effects of using a single compound, and groups EF and EM are the repellent effects of using a repellent formula, where F represents female insects and M represents male insects. It can be seen from the table that the repellent effect of the formula compound on male and female citrus psyllids can reach 5.98 to 6.75, which is significantly higher than the repellent effect of a single compound of 1.80 to 4.07.

[0023] Table 1 Composition of multi-component repellent

[0024]

[0025]

[0026] Table 2 Repellent activity test data

[0027]

[0028] Example 2

[0029] Indoor olfactory electrophysiological experiments of citrus psyllid repellents were conducted at Zhongkai University of Agriculture and Engineering from August 1 to 19, 2023.

[0030] The components of the repellent: nootkatone, carvacrol, thymol and matrine, all have a purity of more than 99%.

[0031] Set up a blank control - air, use n-hexane as the solvent, prepare nootkatone, carvacrol, thymol, and matrine at a concentration of 10 mg / ml. Take 0.1 part of nootkatone, 0.05 part of carvacrol, 4.0 parts of thymol, and 3.0 parts of matrine and mix them to prepare a 10 mg / ml repellent mixed solution. Test with a dose of 10 microliters. Wait for the EAG signal to drive to stabilize before starting to record the antenna potential response value. Each antenna is tested first with a control stimulus, then with a reference stimulus. The standard compounds are randomly selected in order, and finally the reference and control stimuli are tested separately.

[0032] The indoor environment was controlled at a temperature of 25°C and a humidity of 60-75%. The antenna potentiometer was connected to a computer via a DAC-2 signal collector. The airflow passed through the activated carbon and humidifying bottle and entered the glass tube at a flow rate of 100 mL / min, carrying the stimulus source to the antennae, which were placed about 1 cm from the mouth of the glass tube. During the test, a 10-microliter gun tip was inserted into the hole of the glass tube. The stimulation time was 300 ms, the stimulation time interval was 1 min, and each treatment was repeated 10 times. The stimulation airflow was 20 mL / min. The test results are shown in Table 3.

[0033] In the test data of Table 3, groups AD are the physiological activity of using a single compound odor source, and the antennae electrophysiological response value corresponding to the lowest concentration is used; E is the air control without adding odor components; Group G is the effect of the self-prepared citrus psyllid adult repellent composition (multi-component repellent), and the antennae electrophysiological maximum response value is used. It can be seen from the table that after using the citrus psyllid adult repellent composition (multi-component repellent) containing the citrus psyllid adult of this embodiment, the electrophysiological activity of the single compound to the citrus psyllid adult reaches 0.049 to 0.146 millivolts, while the electrophysiological activity of the composition (multi-component repellent) is significantly enhanced, reaching 0.175 millivolts.

[0034] Table 3 Results of electroantennary responses of repellents to citrus psyllids

[0035]

[0036]

[0037] The above are only preferred embodiments of the present invention. It should be noted that the above preferred embodiments should not be regarded as limiting the present invention, and the protection scope of the present invention should be based on the scope defined by the claims. For ordinary technicians in this technical field, several improvements and modifications can be made without departing from the spirit and scope of the present invention, and these improvements and modifications should also be regarded as the protection scope of the present invention.

Claims

1. A repellent composition for citrus psyllid adults, characterized in that: The invention comprises the following components in parts by weight: 0.1 to 1.2 parts of nootkatone, 0.05 to 1.0 parts of carvacrol, 4.0 to 6.5 parts of thymol and 3.0 to 5.0 parts of matrine.

2. The repellent composition for citrus psyllid adults according to claim 1, characterized in that: The repellent composition comprises the following components in parts by weight: 0.5 to 0.8 parts of nootkatone, 0.1 to 0.8 parts of carvacrol, 4.5 to 6.0 parts of thymol, and 3.5 to 4.5 parts of matrine.

3. The repellent composition for citrus psyllid adults according to claim 2, characterized in that: The repellent composition comprises the following components in parts by weight: 0.6 parts of nootkatone, 0.3 parts of carvacrol, 5.1 parts of thymol and 4.0 parts of matrine.

4. Use of the repellent composition for adult citrus psyllids according to claim 1, 2 or 3 in repelling and controlling citrus psyllids.

5. The use according to claim 4, characterized in that: The application is to apply the repellent composition to citrus leaves to directly repel citrus psyllid adults from sucking and laying eggs; or to use the repellent composition in combination with an agricultural nano slow-release agent.

6. A product for repelling and controlling citrus psyllids, characterized in that: A repellent composition comprising the repellent composition of claim 1, 2 or 3.

7. The product according to claim 6, characterized in that The dosage forms of the product include emulsion, powder, aqueous solution, ointment, tincture, cream, aerosol or tablet.

8. A method for controlling and / or monitoring citrus psyllid adults, characterized in that: The repellent composition according to any one of claims 1 to 3 or the product according to claim 6 is applied to the habitat of citrus psyllid to repel citrus psyllid.

9. The method according to claim 8, characterized in that The dosage of the repellent composition is 0.5 to 10 mg / m2.

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