Application of plant hormone in inhibition of fungal growth and synthesis of mycotoxin

Abstract

The invention belongs to the technical field of bioengineering, and particularly relates to application of plant hormones in inhibition of fungal growth and inhibition of mycotoxin synthesis. The invention provides an application of a plant hormone in inhibition of fungal growth and inhibition of synthesis of mycotoxin, the plant hormone is methyl jasmonate, the mycotoxin is deoxynivalenol, and the final concentration of the methyl jasmonate is 30-4000 [mu] M. The invention provides an inhibitor for fusarium graminearum growth and DON synthesis, and provides a botanical inhibitor for formulating a safe, pollution-free and green wheat scab prevention and control strategy.

Description

Technical Field

[0001] The invention belongs to the field of bioengineering technology, and in particular relates to the application of plant hormones in inhibiting fungal growth and inhibiting the synthesis of fungal toxins. Background Art

[0002] Wheat fusarium head blight (FHB) is mainly caused by Fusarium graminearum. Wheat fusarium head blight can cause "white ears" phenomenon, resulting in serious yield losses, and even crop failure in epidemic years. In addition, Fusarium graminearum also produces DON toxins that remain in grains, which will pose a risk to human and animal health. In view of its great harmfulness, wheat fusarium head blight has been included in the "List of Class I Crop Pests and Diseases" by my country's Ministry of Agriculture and Rural Affairs.

[0003] Deoxynivalenol (DON) is a type B triol toxin produced by Fusarium and is the most common contaminant in cereals. DON toxin has serious effects on a variety of important cellular processes in eukaryotes, including inhibition of protein synthesis, damage to membrane structure, and mitochondrial dysfunction. When ingested by humans or animals, it can induce a series of acute and chronic toxic reactions, such as loss of appetite, vomiting, and growth and reproductive dysfunction. DON toxin is also the causative agent of Fusarium during infection of wheat ears through the cob nodes. In addition to causing yield losses, food safety issues caused by DON toxin have also attracted increasing public attention.

[0004] Therefore, protecting crops from DON toxin contamination to ensure food safety is a major production problem that needs to be solved urgently. Finding safe, efficient and green antitoxin compounds is of great significance in the application of agricultural production. Summary of the invention

[0005] The purpose of the present invention is to provide an application of a plant hormone in inhibiting fungal growth and inhibiting the synthesis of fungal toxins. The plant hormone is specifically methyl jasmonate. After methyl jasmonate is added to a PDA culture medium and a liquid toxin-producing culture medium, the growth of Fusarium graminearum and the synthesis of deoxynivalenol can be inhibited. The present invention provides a green and safe plant-derived inhibitor for the growth of Fusarium graminearum and the synthesis of DON.

[0006] To achieve the above object, the technical solution of the present invention is as follows:

[0007] In a first aspect, the present invention provides the use of plant hormones in inhibiting fungal growth and inhibiting the synthesis of fungal toxins, wherein the plant hormone is methyl jasmonate, the fungal toxin is deoxynivalenol, and the final concentration of the methyl jasmonate is 30-4000 μM; the inhibition of fungal growth includes inhibiting the mycelial growth of Fusarium graminearum in PDA culture medium, inhibiting the mycelial growth of Fusarium graminearum in YEPD culture medium, and inhibiting the production of conidia of Fusarium graminearum.

[0008] Preferably, the inhibition of fungal toxin synthesis includes inhibiting the formation of toxigenic bodies of Fusarium graminearum.

[0009] Preferably, the inhibition of mycotoxin synthesis comprises inhibiting TRI gene expression.

[0010] More preferably, the TRI gene includes TRI1 gene, TRI5 gene and TRI12 gene.

[0011] Preferably, the plant hormone is added to a PDA culture medium and a liquid toxin-producing culture medium containing Fusarium graminearum spores, and the culture is statically cultured in the dark;

[0012] 1L of the liquid toxin-producing culture medium contains 0.3g of plant gel, 30g of sucrose, 1g of KH2PO4, 0.5g of MgSO4·7H2O, 0.5g of KCl, 10mg of FeSO4·7H2O, 871mg of arginine, and 20μL of trace elements; 100mL of the trace elements contain 5g of citric acid, 5g of ZnSO4·7H2O, 0.25g of CuSO4·5H2O, 50mg of MnSO4·H2O, 50mg of H3BO3, and 50mg of NaMoO4·2H2O; the pH is adjusted to 5.4-5.6; all the above reagents are purchased from Sigma.

[0013] Preferably, the final concentration of the Fusarium graminearum spores is 1×10 4 Pieces / mL.

[0014] Specifically, methyl jasmonate was added to PDA culture medium, inoculated with a 60 mm diameter bacterial cake, cultured in the dark at 25°C for 3 days, photographed and the colony radius was measured. Methyl jasmonate was added to a liquid toxin-producing culture medium containing Fusarium graminearum spores, cultured at 25°C in the dark for 7 days, and the synthesis of Fusarium graminearum fungal toxins could be inhibited.

[0015] In a second aspect, a method for inhibiting the synthesis of Fusarium graminearum fungal toxins is provided, the method comprising adding methyl jasmonate to a liquid toxin-producing culture medium containing Fusarium graminearum spores, and culturing the medium in a dark environment; wherein:

[0016] The Fusarium graminearum fungal toxin is deoxynivalenol;

[0017] The final concentration of the methyl jasmonate is 30-1000 μM;

[0018] The final concentration of the Fusarium graminearum spores was 1×10 4 Pieces / mL.

[0019] Preferably,

[0020] 1L of the liquid toxin-producing medium contains 0.3g of phytagel, 30g of sucrose, 1g of KH2PO4, 0.5g of MgSO4·7H2O, 0.5g of KCl, 10mg of FeSO4·7H2O, 871mg of arginine, and 20μL of trace elements;

[0021] 100 mL of the trace elements contains 5 g of citric acid, 5 g of ZnSO4·7H2O, 0.25 g of CuSO4·5H2O, 50 mg of MnSO4·H2O, 50 mg of H3BO3, and 50 mg of NaMoO4·2H2O.

[0022] In a third aspect, a plant-derived inhibitor is provided, wherein the plant-derived inhibitor comprises methyl jasmonate, and the methyl jasmonate is used to inhibit the expression of TRI gene and the formation of toxigenic bodies of Fusarium graminearum.

[0023] In a fourth aspect, a plant-derived inhibitor is provided, wherein the plant-derived inhibitor comprises methyl jasmonate, and the methyl jasmonate is used to inhibit the growth of Fusarium graminearum and inhibit the synthesis of fungal toxins.

[0024] Compared with the prior art, the technical solution provided by the present invention has at least the following beneficial effects or advantages:

[0025] The present invention confirms that the content of deoxynivalenol is significantly reduced compared with that of the control group by exogenously adding 30-1000 μM methyl jasmonate to the liquid toxin-producing culture medium, indicating that methyl jasmonate plays an inhibitory function in the synthesis of DON.

[0026] The present invention exogenously adds methyl jasmonate to PDA culture medium and liquid toxin-producing culture medium to confirm that methyl jasmonate can inhibit the growth of Fusarium graminearum and the synthesis of deoxynivalenol. The present invention confirms through quantitative PCR detection that the addition of methyl jasmonate can inhibit the relative expression of TRI gene of Fusarium graminearum, specifically, the addition of methyl jasmonate can inhibit the relative expression of TRI1 gene, TRI5 gene and TRI12 gene of Fusarium graminearum. Microscopic observation found that methyl jasmonate can inhibit the formation of toxin-producing bodies of Fusarium graminearum.

[0027] The present invention provides an inhibitor for the growth of Fusarium graminearum and the synthesis of DON, and provides a plant-based inhibitor for formulating a safe, pollution-free and green strategy for preventing and controlling wheat fusarium rust. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 This is the effect of methyl jasmonate on the growth of Fusarium graminearum; Figure 1 A in the figure is the phenotypic effect of methyl jasmonate on growth; Figure 1 B in the figure is the effect of methyl jasmonate on the growth inhibition rate; Figure 1 C in the figure is the effect of methyl jasmonate on dry weight; Figure 1 D in the figure is the effect of methyl jasmonate on conidia production.

[0029] Figure 2 This is a diagram showing the effect of methyl jasmonate on deoxynivalenol synthesis, TRI gene expression and toxigenic body formation; Figure 2 A in the figure is the effect of methyl jasmonate on DON production; Figure 2 B in the figure is the effect of methyl jasmonate on TRI gene expression; Figure 2 C in the figure shows the effect of methyl jasmonate on the formation of toxigenic bodies. DETAILED DESCRIPTION

[0030] The technical scheme of the present invention is described below in conjunction with the embodiments, but the present invention is not limited to the following embodiments. The experimental methods and detection methods described in each embodiment are conventional methods unless otherwise specified; the reagents and materials described are all commercially available unless otherwise specified.

[0031] Figure 1 A graph showing the effect of methyl jasmonate (MeJA) on the growth of Fusarium graminearum; Figure 1 A in the figure is the phenotypic effect of methyl jasmonate on growth; Figure 1 B in the figure is the effect of methyl jasmonate on the growth inhibition rate; Figure 1 C in the figure is the effect of methyl jasmonate on dry weight; Figure 1 D in the figure is the effect of methyl jasmonate on conidia production. Figure 2 A diagram showing the effect of methyl jasmonate (MeJA) on deoxynivalenol synthesis, TRI gene expression, and toxigenic body formation; Figure 2 A in the figure is the effect of methyl jasmonate on DON production; Figure 2 B in the figure is the effect of methyl jasmonate on TRI gene expression;

[0032] Figure 2 C in the figure shows the effect of methyl jasmonate on the formation of toxin-producing bodies. DIC refers to observation under field conditions; TRI1-GFP refers to the fusion protein of green fluorescent protein (GFP), which contains the coding sequence of the TRI1 gene.

[0033] Example 1

[0034] This example provides that methyl jasmonate inhibits the growth of Fusarium graminearum

[0035] The wild-type strain PH-1 of Fusarium graminearum was activated in a culture dish of PDA medium (purchased from Beijing Aoboxing Biotechnology Co., Ltd.) and cultured in the dark in a constant temperature incubator at 25°C for 3 days. The bacterial cake was punched with a 60mm hole puncher and inoculated into the PDA medium containing methyl jasmonate (purchased from Beijing Aoboxing Biotechnology Co., Ltd.). Methyl jasmonate (purchased from Aladdin) with a final concentration of 30-4000μM was added to the treatment group, and 0.1% ethanol was added to the control group (CK). Cultured in the dark at 25°C for 3d, photographed and the colony radius measured. Figure 1 As shown in A, 30-4000 μM methyl jasmonate inhibited the growth of Fusarium graminearum, and the colony morphology was smaller than that of the control group. Figure 1 As shown in B, the growth inhibition rate of 30-4000 μM methyl jasmonate on Fusarium graminearum is between 4.7% and 100%; moreover, as the final concentration of methyl jasmonate increases, its growth inhibition rate on Fusarium graminearum also increases. When the final concentration of methyl jasmonate increases to 1000 μM, its growth inhibition rate on Fusarium graminearum increases significantly; when the final concentration of methyl jasmonate increases to 4000 μM, its growth inhibition rate on Fusarium graminearum can reach 100%.

[0036] Example 2

[0037] This example provides the results of the study on the inhibition of dry weight of Fusarium graminearum by methyl jasmonate.

[0038] The wild-type strain PH-1 of Fusarium graminearum was activated in a culture dish of PDA medium (purchased from Beijing Aoboxing Biotechnology Co., Ltd.) and cultured in the dark in a constant temperature incubator at 25°C for 3 days. The bacterial block was crushed and placed in CMC liquid culture medium. 1L CMC liquid culture medium contained 1g yeast extract (purchased from OXOID), 1g NH4NO3 (purchased from Shanghai Chemical Reagent Factory), 1g KH2PO4 (purchased from Guangdong Guanghua Technology Co., Ltd.), 0.5g MgSO4·7H2O (purchased from Guangdong Guanghua Technology Co., Ltd.), and 15g carboxymethyl cellulose (purchased from Aladdin). It was shaken in a shaker at a speed of 175rpm and a temperature of 25°C for 3d, and the supernatant was discarded after centrifugation at 3500rpm for 8min to collect the spores. The final concentration was 1×10 4Spore liquid with a concentration of 100 μg / mL was added to YEPD medium. 1L of YEPD liquid medium contains 3g yeast extract, 10g peptone and 20g glucose. Methyl jasmonate (purchased from Aladdin) with a final concentration of 30-4000μM was added to the treatment group, and 0.1% ethanol was added to the control group. The mixture was shaken in a shaker at a speed of 175rpm and a temperature of 25°C for 24 hours. The mycelium was collected by filtering with filter cloth, rinsed three times with sterile water, and then quickly frozen in liquid nitrogen. Freeze-dried for 24 hours using HetoPowerdryer PL3000 (Thermo Company, USA), and the mass was weighed. The results are as follows: Figure 1 As shown in C, 500-4000 μM methyl jasmonate significantly inhibited the dry weight of Fusarium graminearum. Moreover, as the final concentration of methyl jasmonate increased, its inhibition rate on the dry weight of Fusarium graminearum also increased. When the final concentration of methyl jasmonate increased to 1000 μM, its growth inhibition rate on the dry weight of Fusarium graminearum significantly increased; when the final concentration of methyl jasmonate increased to 4000 μM, its growth inhibition rate on the dry weight of Fusarium graminearum was the most significant.

[0039] Example 3

[0040] This example provides that methyl jasmonate inhibits the conidia production of Fusarium graminearum

[0041] The wild-type strain PH-1 of Fusarium graminearum was activated in a culture dish of PDA medium (purchased from Beijing Aoboxing Biotechnology Co., Ltd.) and cultured in the dark in a constant temperature incubator at 25°C for 3 days. The bacterial cakes were punched with a 60mm hole puncher, and 3 bacterial cakes were inoculated into 50 ml of CMC liquid culture medium containing methyl jasmonate. 1L of CMC liquid culture medium contained 1g yeast extract (purchased from OXOID), 1g NH4NO3 (purchased from Shanghai Chemical Reagent Factory), 1g KH2PO4 (purchased from Guangdong Guanghua Technology Co., Ltd.), 0.5g MgSO4·7H2O (purchased from Guangdong Guanghua Technology Co., Ltd.), and 15g carboxymethyl cellulose (purchased from Aladdin). Methyl jasmonate (purchased from Aladdin) was added to the treatment group at a final concentration of 1000 and 4000 μM, and 0.1% ethanol was added to the control group. The culture was shaken in a shaker at a speed of 175 rpm and a temperature of 25°C for 5 days, and the filtrate was collected by filter cloth filtration. The number of spores was counted under an Olympus BX-53 microscope. Figure 1 As shown in D, both 1000 and 4000 μM methyl jasmonate can significantly inhibit the conidia production of Fusarium graminearum, and the inhibitory effect of 4000 μM methyl jasmonate on the conidia production of Fusarium graminearum is more significant.

[0042] Example 4

[0043] This example provides the method for inhibiting the synthesis of deoxynivalenol (DON) by methyl jasmonate.

[0044] Toxigenic culture conditions: The wild-type strain PH-1 of Fusarium graminearum was activated to a culture dish of PDA medium and cultured in a constant temperature incubator at 25°C in the dark for 3 days. The bacterial block was crushed and placed in CMC liquid culture medium. 1L CMC liquid culture medium contained 1g yeast extract (purchased from OXOID), 1g NH4NO3 (purchased from Shanghai Chemical Reagent Factory), 1g KH2PO4 (purchased from Guangdong Guanghua Technology Co., Ltd.), 0.5g MgSO4·7H2O (purchased from Guangdong Guanghua Technology Co., Ltd.), and 15g carboxymethyl cellulose (purchased from Aladdin). Methyl jasmonate (purchased from Aladdin) was added to the treatment group at a final concentration of 30-1000μM, and 0.1% ethanol was added to the control group. The culture was shaken in a shaker at a speed of 175rpm and a temperature of 25°C for 3d. The supernatant was discarded after centrifugation at 3500rpm for 8min to collect spores. Under sterile conditions, 2mL of liquid toxigenic culture medium was added to a 24-well plate. 1L of liquid toxin-producing culture medium contains 0.3g of plant gel, 30g of sucrose, 1g of KH2PO4, 0.5g of MgSO4·7H2O, 0.5g of KCl, 10mg of FeSO4·7H2O, 871mg of arginine, and 20μL of trace elements. Each 100mL of trace elements contains 5g of citric acid, 5g of ZnSO4·7H2O, 0.25g of CuSO4·5H2O, 50mg of MnSO4·H2O, 50mg of H3BO3, and 50mg of NaMoO4·2H2O. All of the above reagents were purchased from Sigma. The pH was adjusted to 5.4-5.6. Methyl jasmonate (purchased from Aladdin) was added to the treatment group at a final concentration of 30-1000μM, and 0.1% of ethanol was added to the control group, and a final concentration of 1×10 4 The spore solution with a concentration of 100 / mL was placed in a constant temperature incubator at 25°C away from light and cultured for 7 days. The bacterial film on the surface of the culture medium was then quickly placed in liquid nitrogen, freeze-dried for 24 hours and weighed.

[0045] DON toxin extraction: Take 400 μL of liquid toxin-producing culture medium after 7 days of culture, add 1600 μL of methanol and mix well, and pass the obtained extract through a C18 SPE solid phase extraction column. Take 800 μL of the filtrate and vacuum concentrate it to dryness, add 50 μL of silanization reagent (the volume ratio of trimethylsilyl imidazole to trimethylchlorosilane is 100:1, purchased from Sigma), vortex thoroughly to mix the silanization reagent and sample thoroughly, and oscillate on an oscillator for 10 minutes. Add 800 μL of isooctane, slightly turn it upside down to mix thoroughly, then add 800 μL of ultrapure water, slightly turn it upside down and let it stand to separate, and transfer the supernatant to the injection bottle.

[0046] GC-MS detection:

[0047] Chromatographic conditions: Rxi-5MS column (30m×0.230-1000μM×0.25μm); split ratio 50:1; injection port temperature 260°C; carrier gas is helium, flow rate 1mL / min; programmed temperature: initial column temperature 150°C, maintained for 1min, increased to 280°C at 30°C / min, and maintained for 15min.

[0048] Mass spectrometry conditions: mass spectrometer interface temperature 280℃; ion source temperature 250℃; injection volume 1μL; m / z 295, 235 and 193 were used for qualitative analysis of DON in SIM mode, and m / z 235 was used for quantitative analysis of DON. The mass spectrometry acquisition time was 5-10min. Figure 2 As shown in A, the content of deoxynivalenol was significantly reduced compared with the control group, indicating that methyl jasmonate can inhibit the synthesis of DON. When the final concentration of methyl jasmonate increased to 1000 μM, its inhibition rate on the dry weight growth of Fusarium graminearum was significantly increased.

[0049] In addition, the examples of the present invention further verified that when the final concentration of methyl jasmonate was increased to 4000 μM, its inhibition rate on the dry weight growth of Fusarium graminearum was most significant.

[0050] Example 5

[0051] This example provides the effect of methyl jasmonate on the expression of TRI gene of Fusarium graminearum

[0052] In the TRI gene, TRI5 catalyzes the cyclization of farnesyl pyrophosphate into trichothecenes and is the first step in the synthesis of DON toxin. The protein encoded by TRI1 catalyzes the formation of C8 trichothecenes from lincomycin and localizes to the toxin-producing body. The transporter encoded by TRI12 is involved in the transport process of DON toxin.

[0053] Add 50 mL of liquid toxin-producing medium to a conical flask and add spores of wild-type Fusarium graminearum strain PH-1 to a final spore concentration of 1 × 10 4 / mL. The treatment group was added with 1000μM methyl jasmonate, and the control group was added with 0.1% ethanol. The mixture was shaken in the dark at 30℃ and 90r / min for 3 days. The mycelium was collected and quickly frozen in liquid nitrogen. Mycelial RNA was extracted, followed by DNA digestion and RNA reverse transcription. Quantitative primers for TRI gene and internal reference gene were designed for quantitative PCR, and the data were then analyzed and processed. The results are shown in the figure. Figure 2 As shown in B, the quantitative primers of TRI gene and internal reference gene are shown in Table 1.

[0054] Table 1: Specific primers for the internal reference gene actin and TRI gene

[0055]

[0056] Depend on Figure 2 As shown in B, methyl jasmonate inhibited the expression of TRI1, TRI5 and TRI12, and their expression levels all decreased by more than 50%.

[0057] Example 6

[0058] This example provides the effect of methyl jasmonate on the formation of toxigenic bodies of Fusarium graminearum

[0059] Toxigenic bodies are considered to be a type of circular protein synthesized by DON. PCR amplification was performed using primers TRI1-GFP-F (5'-AGGGAACAAAAGCTGGGTACCGCTATACTCGGCAGTCCTTTGC-3') and TRI1-GFP-R (5'-GAACAGCTCCTCGCCCTTGCTCACGGCGTCATTTGGGCTTG AGATAG-3') to amplify the TRI1 gene sequence of Fusarium graminearum. The pKNTG plasmid was double-digested with KpnI / HindIII, and the TRI1-GFP fragment was introduced into the cut pKNTG plasmid. PCR verification was performed using pKNTGseq / F (5'-CCCCAGGCTTTACACTTTATGCT-3') and TRI1-GFP-R primers, and the plasmid was sent to the company for sequencing. After confirming that there was no mutation, it was introduced into the protoplasm of PH-1. PCR detection obtained the TRI1-GFP transformed strain of Fusarium graminearum. The bacterial mass of the TRI1-GFP transformed strain was shaken in CMC liquid medium for 3 days (25°C, 200 rpm), filtered through filter cloth and centrifuged, and the supernatant was discarded. 50 mL of liquid toxin-producing medium was added to a conical flask, and spores of the TRI1-GFP strain were added to make the final spore concentration of 1×10 4 The treatment group was added with 1000 μM methyl jasmonate, and the control group was added with 0.1% ethanol. The culture was shaken in the dark for 3 days (25°C, 90 rpm). The mycelium was picked and rinsed with sterile water, and the formation of toxin-producing bodies was observed under a fluorescence microscope. The results are as follows. Figure 2 As shown in C. Figure 2 As shown in C, compared with the control group, no toxigenic bodies could be formed in the hyphae of Fusarium graminearum in the methyl jasmonate-treated group, indicating that methyl jasmonate treatment significantly inhibited the formation of toxigenic bodies.

[0060] As described above, the basic principle, main features and advantages of the present invention are well described. The above embodiments and descriptions are only descriptions of the preferred embodiments of the present invention, and the present invention is not limited by the above embodiments. Without departing from the spirit and scope of the present invention, various changes and improvements made by ordinary technicians in this field to the technical solution of the present invention should fall within the protection scope determined by the present invention.

[0061] The above-described embodiments are part of the embodiments of the present invention, but not all of them. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention claimed for protection, but rather represents selected embodiments of the present invention. All other embodiments obtained without creative work and related deductions and substitutions made by ordinary technicians in the field under the conditions of the concept of the present invention belong to the scope of protection of the present invention.

Claims

1. The use of plant hormones in inhibiting fungal growth and inhibiting the synthesis of fungal toxins, characterized in that: The plant hormone is methyl jasmonate, the fungal toxin is deoxynivalenol, and the final concentration of the methyl jasmonate is 30-4000 μM; The method of inhibiting fungal growth includes inhibiting the mycelial growth of Fusarium graminearum in PDA culture medium, inhibiting the mycelial growth of Fusarium graminearum in YEPD culture medium and inhibiting the production of conidia of Fusarium graminearum.

2. The use according to claim 1, characterized in that: The inhibition of mycotoxin synthesis includes inhibiting the formation of toxin-producing bodies of Fusarium graminearum.

3. The use according to claim 1, characterized in that: The inhibition of mycotoxin synthesis includes inhibiting TRI gene expression.

4. The use according to claim 3, characterized in that: The TRI genes include TRI1 gene, TRI5 gene and TRI12 gene.

5. The use according to claim 1, characterized in that: The plant hormone is added to a PDA culture medium and a liquid toxin-producing culture medium containing Fusarium graminearum spores, and cultured in a static manner in the dark; 1L of the liquid toxin-producing medium contains 0.3g of phytagel, 30g of sucrose, 1g of KH2PO4, 0.5g of MgSO4·7H2O, 0.5g of KCl, 10mg of FeSO4·7H2O, 871mg of arginine, and 20μL of trace elements; 100 mL of the trace elements contains 5 g of citric acid, 5 g of ZnSO4·7H2O, 0.25 g of CuSO4·5H2O, 50 mg of MnSO4·H2O, 50 mg of H3BO3, and 50 mg of NaMoO4·2H2O.

6. The use according to claim 5, characterized in that: The final concentration of the Fusarium graminearum spores was 1×10 4 Pieces / mL.

7. A method for inhibiting the synthesis of Fusarium graminearum fungal toxins, characterized in that: The method comprises adding methyl jasmonate to a liquid toxin-producing culture medium containing spores of Fusarium graminearum, and culturing the medium in a static manner away from light; wherein: The Fusarium graminearum fungal toxin is deoxynivalenol; The final concentration of the methyl jasmonate is 30-1000 μM; The final concentration of the Fusarium graminearum spores was 1×10 4 Pieces / mL.

8. The method according to claim 7, characterized in that 1L of the liquid toxin-producing medium contains 0.3g of phytagel, 30g of sucrose, 1g of KH2PO4, 0.5g of MgSO4·7H2O, 0.5g of KCl, 10mg of FeSO4·7H2O, 871mg of arginine, and 20μL of trace elements; 100 mL of the trace elements contains 5 g of citric acid, 5 g of ZnSO4·7H2O, 0.25 g of CuSO4·5H2O, 50 mg of MnSO4·H2O, 50 mg of H3BO3, and 50 mg of NaMoO4·2H2O.

9. A plant-derived inhibitor, characterized in that: The plant-derived inhibitor includes methyl jasmonate, which is used to inhibit the expression of TRI gene and the formation of toxigenic bodies of Fusarium graminearum.

10. A plant-derived inhibitor, characterized in that: The plant-derived inhibitor includes methyl jasmonate, which is used to inhibit the growth of Fusarium graminearum and inhibit the synthesis of fungal toxins.

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