Microbial preparation for resisting transpiration of crops and application of microbial preparation

Abstract

The invention relates to the field of microbial preparations, and discloses a microbial preparation with crop transpiration resistance and application, the microbial preparation comprises the following components by weight: 20-30 parts of Piriformospora indica, 15-25 parts of Bifidobacterium, 25-35 parts of endophytic fungi, 8-12 parts of auxin, 3-7 parts of cytokinin, 3-7 parts of trace elements, and 2-8 parts of organic matter; according to the microbial preparation for resisting the transpiration of the traditional Chinese medicinal materials, provided by the invention, by combining the alternaria alternata, the piriformospora indica and the bifidobacterium, the stress resistance and the growth efficiency of the traditional Chinese medicinal materials are remarkably enhanced, and the transpiration resistance of the traditional Chinese medicinal materials is enhanced; the method has the advantages that the nutrient absorption and water utilization efficiency of the traditional Chinese medicinal materials is improved by improving a rhizosphere microbial ecological system, so that the optimal growth state is kept in drought or barren soil.

Description

Technical Field

[0001] The present invention relates to the field of microbial preparations, and more particularly to a microbial preparation for resisting transpiration of crops and its application. Background Art

[0002] Transpiration is the process by which water is lost from the surface of living plants (mainly leaves) into the atmosphere in the form of water vapor. It is different from the evaporation process in physics. Transpiration is not only affected by external environmental conditions, but also regulated and controlled by the plants themselves. Therefore, it is a complex physiological process.

[0003] During the crop planting process, especially in arid areas, transpiration has a huge impact on crop growth and will also lead to nutrient loss, especially for some Chinese medicinal crops, which will also affect the efficacy of Chinese medicinal crops. At present, there are many microbial preparations on the market that are used to regulate crop growth, and some can also regulate the crops themselves, but the ingredients of these preparations are complex, and it is difficult to clarify the specific role of each component and their interactions. The anti-transpiration effect is poor, making the effect difficult to predict. Summary of the invention

[0004] In order to solve the above technical problems, the present invention provides a microbial preparation for anti-transpiration of crops and its application.

[0005] The present invention provides a microbial preparation for anti-transpiration of crops, comprising the following components in parts by weight:

[0006] 20-30 parts of Indian pear-shaped spore, 15-25 parts of bifidobacteria, 25-35 parts of endophytic fungi, 8-12 parts of auxin, 3-7 parts of cytokinin, 3-7 parts of trace elements, and 2-8 parts of organic matter.

[0007] A microbial preparation for anti-transpiration of crops, comprising the following components in preferred weight portions:

[0008] 25 parts of Indian pear-shaped spore, 20 parts of bifidobacterium, 30 parts of endophytic fungi, 10 parts of auxin, 5 parts of cytokinin, 5 parts of trace elements, and 5 parts of organic matter.

[0009] A microbial preparation for anti-transpiration of crops, comprising the following components in preferred weight portions:

[0010] 20 parts of Indian pear-shaped spore, 15 parts of bifidobacterium, 25 parts of endophytic fungi, 8 parts of auxin, 3 parts of cytokinin, 3 parts of trace elements, and 2 parts of organic matter.

[0011] A microbial preparation for anti-transpiration of crops, comprising the following components in preferred weight portions:

[0012] 30 parts of Indian pear-shaped spore, 25 parts of bifidobacterium, 35 parts of endophytic fungi, 12 parts of auxin, 7 parts of cytokinin, 7 parts of trace elements, and 8 parts of organic matter.

[0013] Preferably, the endophytic fungus is Alternaria alternata.

[0014] Preferably: the trace elements include zinc, manganese and iron.

[0015] Preferably, the organic matter comprises humic acid.

[0016] A method for preparing a microbial preparation for anti-transpiration of crops comprises the following steps:

[0017] S1: Preparation of culture medium;

[0018] Solid and liquid culture media were prepared for the cultivation of Alternaria alternata, Indian pear-shaped spores and Bifidobacterium, respectively;

[0019] S2: microbial culture;

[0020] Inoculate strains of Alternaria alternata, Indian pear-shaped spores and Bifidobacterium into corresponding culture medium, and culture them under suitable temperature and humidity conditions for a time period that ensures that the bacteria reach the logarithmic growth phase according to the characteristics of the strains;

[0021] S3: pretreatment of organic substrate;

[0022] Mix the humic acid or compost evenly;

[0023] S4: Microbial mixture;

[0024] The cultured Alternaria alternata, Indian pear-shaped spore and Bifidobacterium suspension are mixed in proportion;

[0025] S5: addition of growth regulators;

[0026] The auxin and cytokinin are dissolved in the trace element solution according to the proportion, and then evenly mixed into the microbial mixture;

[0027] S6: adsorption and drying;

[0028] Spray the mixture onto the pretreated organic substrate to allow it to fully absorb, and then slowly dry it in a well-ventilated environment to avoid excessive temperature that may cause loss of microbial activity.

[0029] S7: Finished product;

[0030] The dried mixture is crushed into uniform particles or powder, and packed into light-proof sealed bags;

[0031] S8: Storage and transportation;

[0032] Store the finished product in a cool, dry place, avoid high temperature or humidity to maintain the activity of the bacteria. Try to avoid direct sunlight and high temperature conditions during transportation.

[0033] Preferably, in step S3, after the humic acid or compost is uniformly mixed, the pH value is adjusted to 7.

[0034] The invention discloses an application of a microbial preparation for crop anti-transpiration, which is applied to crop planting in arid and water-deficient areas.

[0035] The Indian pear-shaped spore used in the present invention is a common soil fungus belonging to the genus Fusarium and widely present in various soil environments. It plays an important role in the growth and development of plant roots, especially in improving the resistance of plants to drought and pathogenic microorganisms.

[0036] Promote root growth: Indian pear-shaped spore stimulates the growth and branching of plant roots by secreting plant hormones such as auxin, enhancing the absorption capacity of the roots, thereby improving the plant's acquisition of water and nutrients.

[0037] Enhanced disease resistance: The bacteria can activate the signal pathway of endogenous plant hormones, thereby improving the plant's resistance to pathogenic microorganisms (such as Fusarium oxysporum and Verticillium dahliae) and reducing the occurrence of diseases.

[0038] Improved drought resistance: Under drought conditions, Indian pear-shaped spores can promote the synthesis of endogenous hormones in plants, enhance the drought resistance of plants, reduce transpiration, and help plants grow in water-deficient environments.

[0039] The bifidobacteria used in the present invention are an important class of probiotics, which are widely used in the fields of food and agriculture. They are usually found in the intestines of animals and humans, and can also be found in the soil. Bifidobacteria play an important role in improving plant health and soil microbial communities.

[0040] Improve soil microbial flora: Bifidobacteria improve the soil's microbial environment by decomposing organic matter and producing organic acids, promoting the growth of other beneficial microorganisms and enhancing soil health and fertility.

[0041] Promote root growth: Bifidobacterium can stimulate the development of plant roots by secreting plant hormones (such as auxin) and enhance the root system's absorption of water and nutrients.

[0042] Enhanced nutrient absorption: They help plants absorb nutrients such as nitrogen, phosphorus, and potassium more efficiently, increasing plant growth rate and yield.

[0043] The Alternaria used in the present invention is an important endophytic fungus that is widely present in a variety of plants, especially in Chinese medicinal plants, and has a significant promoting effect on their growth and health. The following is a detailed explanation of the mechanism of action of Alternaria on Chinese medicinal plants:

[0044] 1. Growth promotion

[0045] Alternaria can promote the growth of Chinese medicinal plants in the following ways:

[0046] Production of plant hormones: Alternaria can synthesize a variety of plant hormones, such as auxin, cytokinin, etc. These hormones promote the growth of roots, stems and leaves, and increase the growth rate and stress resistance of plants.

[0047] Improved absorption of nutrients: Alternaria can form a symbiotic relationship with plant roots through its hyphae, enhancing the plant's absorption of nutrients in the soil, especially key elements such as nitrogen, phosphorus, and potassium. This symbiotic relationship not only improves the plant's ability to obtain nutrients, but also enhances its adaptability to adverse environments.

[0048] 2. Enhanced stress resistance

[0049] Alternaria has a significant effect on improving the stress resistance of Chinese medicinal plants, mainly reflected in:

[0050] Water use efficiency: Alternaria can improve the water use efficiency of plants and reduce water loss caused by transpiration. Under adverse conditions such as drought or high temperature, the presence of Alternaria can help plants retain water and increase their survival rate.

[0051] Disease resistance: Alternaria can inhibit the growth and reproduction of certain plant pathogens by producing antibacterial substances, thereby reducing the incidence of plant diseases. This biological control ability is particularly important in the cultivation of Chinese medicinal materials, helping to reduce the use of pesticides.

[0052] 3. Promote root development

[0053] Alternaria is also extremely important for promoting the root system:

[0054] Improvement of root structure: The hyphae of Alternaria can combine with the plant roots to form a more developed root network, increase the surface area of ​​the roots, and thus improve the plant's water absorption capacity and nutrient absorption efficiency.

[0055] Regulation of rhizosphere microbial communities: Alternaria improves the rhizosphere environment, promotes the growth of beneficial microorganisms, inhibits the reproduction of pathogenic microorganisms, forms a good rhizosphere ecosystem, and further improves the overall health of plants.

[0056] 4. Role of metabolites

[0057] In its interaction with plants, Alternaria produces a variety of metabolites that not only directly promote plant growth but also affect the physiological metabolic processes of plants. For example:

[0058] Secretion of organic acids: Alternaria can secrete organic acids, which can help dissolve minerals in the soil and release more nutrients for plants to absorb.

[0059] Production of bioactive substances: Alternaria can produce a variety of bioactive substances, which help to enhance the stress resistance of plants, promote the activity of antioxidant enzymes in plants, and reduce oxidative stress.

[0060] In summary, Alternaria alternata significantly promotes the growth and health of Chinese medicinal plants and enhances their stress resistance and disease resistance through multiple mechanisms such as promoting the synthesis of plant hormones, enhancing nutrient absorption, improving water use efficiency, improving root development and secreting a variety of beneficial metabolites.

[0061] In the microbial preparation of the present invention, the combination of Indian pear-shaped spore and bifidobacterium can form a synergistic effect:

[0062] Root health: Indian pear-shaped spores promote root growth by activating endogenous plant hormones, while Bifidobacterium further improves the soil environment and enhances the absorption capacity of the roots.

[0063] Enhanced stress resistance: The two work together to improve the plant's resistance to biotic and abiotic stresses (such as drought and disease), ensuring that Chinese medicinal materials can still grow healthily in harsh environments.

[0064] This combination not only helps to improve the growth efficiency of Chinese medicinal materials, but also effectively reduces water consumption and achieves the purpose of water saving.

[0065] In the microbial preparation of the present invention, the combination of Alternaria alternata and Indian pear-shaped spore can form a synergistic effect:

[0066] The combined action of Alternaria alternata and Pyriformis indica can form a benign rhizosphere microbial environment, and the two promote each other in the following ways:

[0067] Enhanced effects: Alternaria promotes root growth and stress resistance, while P. indica increases nutrient uptake and activates defense mechanisms. The combination of the two makes plants more tolerant to transpiration.

[0068] Resource sharing: In a symbiotic situation, Alternaria alternata and P. indica can share nutrients and water in the soil, forming a complementary relationship and improving the overall growth efficiency of the plant.

[0069] Comprehensive regulation: By regulating the physiological state of the plant, the two work together to regulate stomatal pores, optimize water utilization, and reduce water loss caused by transpiration.

[0070] In conclusion, the synergistic effect between Alternaria alternata and P. indica provides a better growth environment for TCM plants, thus playing an important role in transpiration inhibition and plant health.

[0071] In the microbial preparation of the present invention, the combination of Alternaria and Bifidobacterium can form a synergistic effect:

[0072] Synthesis of plant hormones:

[0073] Alternaria can produce plant hormones such as auxin and cytokinin, which promote the growth of plant roots, stems and leaves. Bifidobacterium, through its metabolites, may promote the synthesis of plant hormones, further enhancing the growth potential of plants.

[0074] Microbial community interactions:

[0075] Alternaria and Bifidobacterium interact in the rhizosphere to form a complex microbial community that enhances plant adaptability to the environment. This symbiotic relationship can improve plant nutrient absorption, especially in nutrient-poor soils.

[0076] Effective utilization of nutrients:

[0077] Alternaria improves the plant's ability to absorb nutrients such as nitrogen, phosphorus, and potassium through its hyphae network, while Bifidobacterium can improve the intestinal microbial environment and enhance the plant's digestion and utilization of nutrients.

[0078] Enhanced disease resistance:

[0079] Alternaria can produce antibiotics and other antibacterial substances to reduce the infection of pathogens, while Bifidobacteria can enhance the resistance of plants to diseases by promoting the immune response of plants. This synergistic effect helps reduce the use of chemical pesticides.

[0080] Balance of the rhizosphere microbial community:

[0081] Alternaria and Bifidobacterium co-exist in the rhizosphere environment, where they can compete with each other for pathogenic microorganisms and inhibit their growth, thereby forming a beneficial microbial ecosystem and improving the health of plant roots.

[0082] Bifidobacteria are excellent at breaking down organic matter, releasing nutrients that can be absorbed by plants, while Alternaria can enhance the structural stability of the soil, thereby promoting root growth.

[0083] Secretion of organic acids:

[0084] Alternaria secretes organic acids that help dissolve minerals in the soil, releasing more nutrients for plants to absorb. Bifidobacteria also produce organic acids, further promoting soil fertility and plant nutrient absorption.

[0085] Synthesis of biologically active substances:

[0086] The metabolites of the two can promote each other. Some metabolites of Alternaria may enhance the growth of Bifidobacterium, while the metabolites of Bifidobacterium may also stimulate the activity of Alternaria, thus forming a virtuous feedback loop.

[0087] Alternaria and Bifidobacterium form a synergistic effect through multiple mechanisms such as promoting the synthesis of plant hormones, improving the rhizosphere microbial environment, enhancing plant stress resistance, and increasing the utilization efficiency of nutrients, which significantly promotes the growth and health of Chinese medicinal plants.

[0088] In the microbial preparation of the present invention, Alternaria alternata, Indian pear-shaped spore and Bifidobacterium have synergistic effects. The following is the specific synergistic mechanism of the three:

[0089] Comprehensively improve plant stress resistance:

[0090] Alternaria and Indian Pear-Shaped Spore can work together to enhance the stress resistance of plants. Alternaria improves tolerance by promoting the synthesis of stress-resistant proteins, while Indian Pear-Shaped Spore activates plant defense mechanisms to improve resistance to environmental stress. Bifidobacterium further promotes the stability of plant physiological state, making plants more adaptable when facing challenges such as drought and salt stress.

[0091] Optimizes Nutrient Utilization and Absorption:

[0092] Indian pear-shaped spores help plants absorb nutrients that are difficult to obtain through their hyphae, while Bifidobacterium improves the availability of nutrients by decomposing organic matter in the soil. Alternaria adjusts the root structure and increases the effective absorption area, thus forming a combined force of the three in promoting plant nutrient absorption and maximizing nutrient utilization.

[0093] Construction of rhizosphere microbial ecosystem:

[0094] Together, the three create a favorable microbial community in the plant rhizosphere. The hyphae of Alternaria and Pyriformis indica can interweave with each other to form a physical support structure that promotes the colonization and reproduction of beneficial bacteria such as Bifidobacterium, thereby inhibiting the invasion of pathogens and improving the overall health of the plant.

[0095] Regulates water use efficiency of plants:

[0096] Alternaria and Pyriformis indica work together to optimize the water balance of plants through their metabolites that affect the openness of plant stomata. The presence of Bifidobacterium further ensures the health of the rhizosphere, allowing water to be transferred and used more efficiently from the soil.

[0097] Synergistically promote plant growth:

[0098] In terms of plant growth hormone regulation, Alternaria alternata and Pyriformis indica can promote the synthesis of auxins and cytokinins, leading to rapid growth of roots and aerial parts. The metabolites of Bifidobacterium may enhance the efficacy of these hormones, making the growth-promoting effect more significant.

[0099] Through such synergistic effects, Alternaria alternata, Indian pear-shaped spores and Bifidobacterium jointly improve the adaptability of Chinese medicinal plants to the environment, nutrient absorption capacity and disease resistance, forming a microecological support system with multi-dimensional advantages. This integrated microbial combination provides comprehensive support for the healthy growth of Chinese medicinal plants, while reducing the need for external chemical inputs.

[0100] The beneficial effects of the present invention are as follows: the microbial preparation for crop anti-transpiration proposed in the present invention significantly enhances the stress resistance and growth efficiency of Chinese medicinal plants by combining Alternaria alternata, Indian pear-shaped spore and Bifidobacterium, and enhances the anti-transpiration effect of Chinese medicinal materials. Its advantage is that the nutrient absorption and water utilization efficiency of Chinese medicinal materials are improved by improving the rhizosphere microbial ecosystem, thereby maintaining the best growth state in arid or barren soils. BRIEF DESCRIPTION OF THE DRAWINGS

[0101] Figure 1 This is a curve diagram of the transpiration rate change in Example 6 of the present invention. DETAILED DESCRIPTION

[0102] The subject matter described herein will now be discussed with reference to example embodiments. It should be understood that the discussion of these embodiments is only to enable those skilled in the art to better understand and implement the subject matter described herein, and the functions and arrangements of the elements discussed may be changed without departing from the scope of protection of the contents of this specification. Each example may omit, replace or add various processes or components as needed. In addition, the features described relative to some examples may also be combined in other examples.

[0103] Example 1

[0104] In this embodiment, a microbial preparation for anti-transpiration of crops is proposed, which includes the following components in parts by weight:

[0105] 25 parts of Indian pear-shaped spore, 20 parts of bifidobacterium, 30 parts of endophytic fungi, 10 parts of auxin, 5 parts of cytokinin, 5 parts of trace elements, and 5 parts of organic matter.

[0106] Among them: the endophytic fungus is Alternaria alternata. Trace elements include zinc, manganese and iron. Organic matter includes humic acid.

[0107] Example 2

[0108] In this embodiment, a microbial preparation for anti-transpiration of crops is proposed, which includes the following components in parts by weight:

[0109] 20 parts of Indian pear-shaped spore, 15 parts of bifidobacterium, 25 parts of endophytic fungi, 8 parts of auxin, 3 parts of cytokinin, 3 parts of trace elements, and 2 parts of organic matter.

[0110] Among them: the endophytic fungus is Alternaria alternata. Trace elements include zinc, manganese and iron. Organic matter includes humic acid.

[0111] Example 3

[0112] In this embodiment, a microbial preparation for anti-transpiration of crops is proposed, which includes the following components in parts by weight:

[0113] 30 parts of Indian pear-shaped spore, 25 parts of bifidobacterium, 35 parts of endophytic fungi, 12 parts of auxin, 7 parts of cytokinin, 7 parts of trace elements, and 8 parts of organic matter.

[0114] Among them: the endophytic fungus is Alternaria alternata. Trace elements include zinc, manganese and iron. Organic matter includes humic acid.

[0115] Example 4

[0116] In this embodiment, a method for preparing a microbial preparation for crop anti-transpiration is proposed, comprising the following steps:

[0117] S1: Preparation of culture medium;

[0118] Solid and liquid culture media were prepared for the cultivation of Alternaria alternata, Indian pear-shaped spores and Bifidobacterium, respectively;

[0119] S2: microbial culture;

[0120] Inoculate strains of Alternaria alternata, Indian pear-shaped spores and Bifidobacterium into corresponding culture medium, and culture them under suitable temperature and humidity conditions for a time period that ensures that the bacteria reach the logarithmic growth phase according to the characteristics of the strains;

[0121] S3: pretreatment of organic substrate;

[0122] Mix humic acid or compost evenly and adjust to pH 7;

[0123] S4: Microbial mixture;

[0124] The cultured Alternaria alternata, Indian pear-shaped spore and Bifidobacterium suspension are mixed in proportion;

[0125] S5: addition of growth regulators;

[0126] The auxin and cytokinin are dissolved in the trace element solution according to the proportion, and then evenly mixed into the microbial mixture;

[0127] S6: adsorption and drying;

[0128] Spray the mixture onto the pretreated organic substrate to allow it to fully absorb, and then slowly dry it in a well-ventilated environment to avoid excessive temperature that may cause loss of microbial activity.

[0129] S7: Finished product;

[0130] The dried mixture is crushed into uniform particles or powder, and packed into light-proof sealed bags;

[0131] S8: Storage and transportation;

[0132] Store the finished product in a cool, dry place, avoid high temperature or humidity to maintain the activity of the bacteria. Try to avoid direct sunlight and high temperature conditions during transportation.

[0133] Example 5

[0134] In this embodiment, an application of a microbial preparation for crop anti-transpiration is proposed, which is applied to crop planting in arid and water-deficient areas, especially Chinese medicinal material planting.

[0135] Example 6

[0136] This example is used to investigate the inhibitory effect of the microbial preparation (prepared using the components disclosed in Example 2 and the method disclosed in Example 4) on the transpiration of Chinese medicinal plants. The effect of the microbial preparation was evaluated by measuring the transpiration rate of plants under different conditions. The control test setting included only applying traditional fertilizers and applying microbial preparations, so that the experiment could clearly show the comparative effects of the two.

[0137] 1. Experimental materials and equipment:

[0138] Chinese medicinal plant seedlings: Astragalus.

[0139] Microbial preparation (prepared using the components disclosed in Example 2 and the method disclosed in Example 4).

[0140] Traditional fertilizers.

[0141] Weighing equipment.

[0142] Transpiration measurement device: lysimeter.

[0143] Temperature and humidity recorder.

[0144] Sample basin.

[0145] Measuring cylinder and measuring cup.

[0146] 2. Experimental Procedure

[0147] 2.1 Experimental and control group settings:

[0148] The Chinese medicinal plant seedlings were divided into two groups: an experimental group (administered with microbial preparations) and a control group (administered with the same amount of traditional fertilizers).

[0149] 2.2 Matrix preparation:

[0150] The sample pots were filled with the same soil matrix. The experimental group mixed the corresponding amount of microbial preparations into the soil, while the control group used traditional fertilizers.

[0151] 2.3 Plant planting:

[0152] Plant the seedlings in the prepared sample pots, with plants of the same size in each pot, and mark them clearly.

[0153] 2.4 Adaptation phase:

[0154] The plants were placed in a greenhouse environment for 7 days to recover and adapt to avoid the effects of transplanting stress.

[0155] 2.5 Environmental condition control:

[0156] The temperature and humidity levels in the greenhouse were monitored and controlled using a temperature and humidity logger to ensure a consistent experimental environment.

[0157] 2.6 Transpiration measurement:

[0158] The transpiration rate (mL / h) of each group of plants was measured daily using a transpiration measuring device, and the results were recorded for two weeks.

[0159] Measurements are taken simultaneously every day to eliminate interference from natural evaporation and environmental changes.

[0160] 2.7 Data Recording:

[0161] All experimental data were summarized and the average daily transpiration rates of the experimental group and the control group were calculated respectively.

[0162] 3. Experimental results

[0163] As attached Figure 1 The transpiration rate change curve is shown. The horizontal axis is the number of experimental days (1-7 days), and the vertical axis is the transpiration rate (mL / h).

[0164] 4. Experimental Summary

[0165] This experiment verified the inhibitory effect of microbial preparations on the transpiration of Chinese medicinal materials. The transpiration rate of the experimental group was significantly lower than that of the control group. This shows that the microbial preparation of the present invention can not only enhance the stress resistance and nutrient absorption capacity of plants, but also slow down water loss to a certain extent.

[0166] Example 7

[0167] This example is used to verify the synergistic effect of Alternaria alternata and Pyriformis indica in promoting the growth of Chinese medicinal plants and improving their stress resistance. By comparing the effects of the two bacterial communities applied alone and in combination on plant growth indicators, it is determined whether there is a synergistic effect between the two.

[0168] 1. Materials and Equipment

[0169] Chinese medicinal plant seedlings: Astragalus.

[0170] Alternaria culture medium.

[0171] Indian pear-shaped spore culture medium.

[0172] Control group bacterial solution: invalid carrier.

[0173] Incubator or greenhouse.

[0174] Balance, measuring cylinder.

[0175] Labeled sample basins.

[0176] Equipment for measuring transpiration rate.

[0177] 2. Experimental Procedure

[0178] 2.1 Experimental group settings:

[0179] Four experimental groups were set up: Alternaria group, Indian Piriformis group, combined bacteria group (Alternaria and Indian Piriformis mixed) and control group (only ineffective carrier was applied).

[0180] 2.2 Matrix preparation:

[0181] Fill the sample pots with the same sterile soil to ensure consistent conditions across groups.

[0182] 2.3 Vaccination:

[0183] Alternaria alternata, Indian pear-shaped spore and the mixture of the two were inoculated into the corresponding groups at the same bacterial density, and the same amount of bacterial solution was applied to each pot.

[0184] 2.4 Plant cultivation:

[0185] Astragalus seedlings were evenly planted in sample pots of different treatment groups and clearly marked.

[0186] 2.5 Management during the growth period:

[0187] The sample pots were placed in a greenhouse, where light, temperature, and humidity were controlled, and water was checked and added daily.

[0188] 2.6 Data Collection:

[0189] At two and four weeks after planting, the following parameters were measured:

[0190] Plant height and leaf area (to assess growth);

[0191] biomass (harvesting some plants for dry weight analysis);

[0192] Transpiration rate (using transpiration measurement equipment).

[0193] 3. Experimental results

[0194] Growth indicator comparison table:

[0195] Group Plant height (cm) <![CDATA[Leaf area (cm 2 )]]> Dry weight (g) Transpiration rate (mL / h) Alternaria group 15.2 35.4 3.2 5.6 Piriformis indica 14.8 34.7 3.0 5.8 Combined bacterial groups 16.5 39.3 3.8 5.0 Control group 13.5 30.2 2.7 6.2

[0196] 4. Experimental Summary

[0197] The experimental results showed that the combined application of Alternaria alternata and Indian pear-shaped spores significantly improved the growth index and stress resistance of Astragalus membranaceus. Compared with the single strain group and the control group, the plant height, leaf area and biomass of the combined group increased significantly, and the transpiration rate decreased significantly. This shows that Alternaria alternata and Indian pear-shaped spores have obvious synergistic effects on plant growth and water utilization. The comprehensive data show that the combination of the two can effectively promote the growth and adaptability of Chinese medicinal plants.

[0198] Example 8

[0199] This example is used to verify the synergistic effect of Alternaria and Bifidobacterium in promoting the growth of Chinese medicinal plants and improving soil health. The effects on plant growth indicators, soil microbial communities and plant nutrient absorption were observed by treating with different combinations of strains.

[0200] 1. Materials and Equipment

[0201] Chinese medicinal plant seedlings: Astragalus.

[0202] Alternaria culture medium.

[0203] Bifidobacterium culture fluid.

[0204] Control group culture medium (ineffective vector).

[0205] Greenhouse equipment.

[0206] Balance, measuring cylinder.

[0207] Filter paper and absorbent paper.

[0208] Labeled sample basins and test tubes.

[0209] Equipment for testing nitrogen, phosphorus and potassium content.

[0210] 2. Experimental Procedure

[0211] 2.1 Experimental group settings:

[0212] Four experimental groups were set up: Alternaria group, Bifidobacterium group, combined bacteria group (Alternaria mixed with Bifidobacterium), and control group (only ineffective carrier was administered).

[0213] 2.2 Matrix preparation:

[0214] Fill the sample pots with the same sterile soil to ensure consistent conditions across groups.

[0215] 2.3 Vaccination:

[0216] Alternaria, Bifidobacterium, and a mixture of the two were inoculated into the corresponding groups at the same bacterial density, and an equal amount of bacterial solution was applied to each pot.

[0217] 2.4 Plant cultivation:

[0218] Astragalus seedlings were evenly planted in sample pots of different treatment groups and clearly marked.

[0219] 2.5 Management during the growth period:

[0220] The sample pots were placed in a greenhouse, where light, temperature, and humidity were controlled, and water was checked and added daily.

[0221] 2.6 Data Collection:

[0222] Four weeks after planting, the following parameters were measured:

[0223] Plant height and leaf area (to assess growth).

[0224] Diversity of soil microbial communities (analyzed by 16S rRNA sequencing of soil samples).

[0225] Plant nitrogen, phosphorus and potassium content (samples were taken from each plant for chemical analysis).

[0226] 3. Experimental results

[0227] Growth indicators and soil analysis comparison table

[0228]

[0229] 4. Experimental Summary

[0230] The experimental results showed that the combined effect of Alternaria and Bifidobacterium showed significant advantages in all growth and soil health indicators. Compared with the single strain and the control group, the plant height and leaf area of ​​the combined group increased significantly, the soil microbial diversity also increased significantly, and the plant's nitrogen, phosphorus, and potassium absorption capacity increased. This shows that there is a synergistic effect between Alternaria and Bifidobacterium, and they have obvious complementarity in promoting plant growth and improving soil fertility.

[0231] Example 9

[0232] This example is used to verify the synergistic effect of Indian pear-shaped spores and bifidobacteria on the growth and disease resistance of Chinese medicinal plants. The synergistic effect of the two microorganisms is evaluated by comparing the effects of the two microorganisms used alone and in combination on plant growth indicators, disease resistance and soil health.

[0233] 1. Materials and Equipment

[0234] Chinese medicinal plant seedlings: licorice.

[0235] Indian pear-shaped spore culture medium.

[0236] Bifidobacterium culture fluid.

[0237] Control group culture medium (ineffective vector).

[0238] Temperature-controlled incubator or greenhouse.

[0239] Plant pathogens (Fusarium oxysporum, for disease resistance testing).

[0240] Balance, measuring cylinder.

[0241] Sample basin.

[0242] Plant growth measurement tools (e.g., tape measure, calipers).

[0243] Disease indicator measurement tool.

[0244] 2. Experimental Procedure

[0245] 2.1 Experimental group settings:

[0246] Four experimental groups were set up: P. indica group, Bifidobacterium group, combined bacteria group (P. indica mixed with Bifidobacterium) and control group (only ineffective carrier was administered).

[0247] 2.2 Matrix preparation:

[0248] Fill the sample pots with the same sterile soil to ensure consistent conditions across groups.

[0249] 2.3 Vaccination:

[0250] Indian pear-shaped spore, Bifidobacterium, and the mixture of the two were inoculated into the corresponding groups at the same bacterial density, and the same amount of bacterial solution was applied to each pot.

[0251] 2.4 Plant cultivation:

[0252] Licorice seedlings were evenly planted in sample pots of different treatment groups and clearly marked.

[0253] 2.5 Management during the growth period:

[0254] The sample pots were placed in a greenhouse with controlled light, temperature, and humidity, and were checked and water was added daily.

[0255] 2.6 Disease resistance test:

[0256] Two weeks after planting, plant pathogens were inoculated on plant leaves and the development of symptoms was observed.

[0257] 2.7 Data Collection:

[0258] Four weeks after planting, the following parameters were measured:

[0259] Plant height and leaf area (to assess growth).

[0260] The extent of the disease (measured by lesion area).

[0261] Soil health indicators (microbial diversity and soil enzyme activity).

[0262] 3. Experimental results

[0263] Growth index and disease resistance comparison table

[0264] Group Plant height (cm) <![CDATA[Leaf area (cm 2 )]]> <![CDATA[Lesion area (cm 2 )]]> Soil enzyme activity (unit) Piriformis indica 15.2 38.1 4.5 120 Bifidobacterium group 15.0 37.5 4.8 115 Combined bacterial groups 16.3 40.2 3.2 135 Control group 14.0 34.0 6.0 100

[0265] 4. Experimental Summary

[0266] The experimental results showed that the combined use of Indian pear-shaped spores and Bifidobacterium had a significant synergistic effect on the growth and disease resistance of Chinese medicinal plants. Compared with the single strain and the control group, the plant height and leaf area of ​​the combined group increased significantly, and the lesion area decreased, indicating enhanced disease resistance. In addition, the increase in soil enzyme activity reflects the improvement in soil health. This synergistic effect illustrates the significant role of the two microorganisms in jointly promoting plant health, improving disease resistance, and enhancing soil functions.

[0267] Example 10

[0268] This example is used to verify the synergistic effect of Alternaria alternata, Indian pear-shaped spores and Bifidobacterium on the growth and health of Chinese medicinal plants. By observing the effects of these three microorganisms used alone and in combination on plant growth performance, stress resistance and soil microecology, their synergistic effect and comprehensive improvement effect on plants are determined.

[0269] 1. Materials and Equipment

[0270] Chinese medicinal plant seedlings: Astragalus.

[0271] Alternaria culture medium.

[0272] Indian pear-shaped spore culture medium.

[0273] Bifidobacterium culture fluid.

[0274] Control group culture medium (ineffective vector).

[0275] Temperature-controlled incubator or greenhouse.

[0276] Balance, measuring cylinder.

[0277] Labeled sample basins.

[0278] Plant growth measurement tools (e.g., tape measure, calipers).

[0279] Soil nutrient analysis equipment (such as N, P, K analyzers).

[0280] Kit for assaying antioxidant enzyme activity in plants.

[0281] 2. Experimental Procedure

[0282] 2.1 Experimental group settings:

[0283] Five experimental groups were set up: Alternaria group, Indian pear-shaped spore group, Bifidobacterium group, three-bacteria combination group (Alternaria, Indian pear-shaped spore and Bifidobacterium mixed), and control group (only ineffective carrier was administered).

[0284] 2.2 Matrix preparation:

[0285] Sample pots were filled uniformly with sterile soil to ensure consistent conditions among different treatment groups.

[0286] 2.3 Vaccination:

[0287] Each bacterial species and the mixture of the three bacteria were inoculated into the soil of the corresponding group at the same bacterial density, and the same amount of bacterial solution was applied to each pot.

[0288] 2.4 Plant cultivation:

[0289] Astragalus seedlings were evenly planted in pots with different treatments and clearly marked.

[0290] Management during the growth period:

[0291] The sample pots were placed in a greenhouse, where light, temperature and humidity were controlled, and water was added regularly to ensure the same growth environment.

[0292] 2.5 Data Collection:

[0293] Four weeks after planting, measure the following:

[0294] Plant height and leaf area (to assess growth).

[0295] Soil nutrient content (N, P, K).

[0296] Antioxidant enzyme activity (peroxidase, POD) of plants.

[0297] 3. Experimental results

[0298] Growth indicators and soil analysis comparison table

[0299]

[0300] 4. Experimental Summary

[0301] The experimental results clearly showed that the combined use of Alternaria alternata, Indian pear-shaped spores and Bifidobacterium had a significant synergistic effect on the growth of Chinese medicinal plants, soil nutrient improvement and antioxidant capacity enhancement. Compared with the single-species group and the control group, the three-bacteria combination group showed the best results in all biological parameters, indicating that these microorganisms not only promote the healthy growth of plants individually, but also produce an enhanced effect through complex ecological interactions.

[0302] The above describes an embodiment of the present embodiment, but the present embodiment is not limited to the above-mentioned specific implementation methods. The above-mentioned specific implementation methods are merely illustrative and not restrictive. Under the guidance of the present embodiment, ordinary technicians in this field can also make many forms, all of which are protected by the present embodiment.

Claims

1. A microbial preparation for anti-transpiration of crops, characterized in that: The composition comprises the following components in parts by weight: 20-30 parts of Indian pear-shaped spore, 15-25 parts of bifidobacteria, 25-35 parts of endophytic fungi, 8-12 parts of auxin, 3-7 parts of cytokinin, 3-7 parts of trace elements, and 2-8 parts of organic matter.

2. The microbial preparation for anti-transpiration of crops according to claim 1, characterized in that: The composition comprises the following components in parts by weight: 25 parts of Indian pear-shaped spore, 20 parts of bifidobacterium, 30 parts of endophytic fungi, 10 parts of auxin, 5 parts of cytokinin, 5 parts of trace elements, and 5 parts of organic matter.

3. The microbial preparation for anti-transpiration of crops according to claim 1, characterized in that: The composition comprises the following components in parts by weight: 20 parts of Indian pear-shaped spore, 15 parts of bifidobacterium, 25 parts of endophytic fungi, 8 parts of auxin, 3 parts of cytokinin, 3 parts of trace elements, and 2 parts of organic matter.

4. The microbial preparation for anti-transpiration of crops according to claim 1, characterized in that: The composition comprises the following components in parts by weight: 30 parts of Indian pear-shaped spore, 25 parts of bifidobacterium, 35 parts of endophytic fungi, 12 parts of auxin, 7 parts of cytokinin, 7 parts of trace elements, and 8 parts of organic matter.

5. The microbial preparation for anti-transpiration of crops according to claim 1, characterized in that: The endophytic fungus is Alternaria alternata.

6. The microbial preparation for anti-transpiration of crops according to claim 1, characterized in that: The trace elements include zinc, manganese and iron.

7. The microbial preparation for anti-transpiration of crops according to claim 1, characterized in that: The organic matter includes humic acid.

8. The microbial preparation for anti-transpiration of crops according to claim 1, characterized in that: The preparation method of the microbial preparation comprises the following steps: S1: Preparation of culture medium; Solid and liquid culture media were prepared for the cultivation of Alternaria alternata, Indian pear-shaped spores and Bifidobacterium, respectively; S2: microbial culture; Inoculate strains of Alternaria alternata, Indian pear-shaped spores and Bifidobacterium into corresponding culture medium, and culture them under suitable temperature and humidity conditions for a time period that ensures that the bacteria reach the logarithmic growth phase according to the characteristics of the strains; S3: pretreatment of organic substrate; Mix the humic acid or compost evenly; S4: Microbial mixture; The cultured Alternaria alternata, Indian pear-shaped spore and Bifidobacterium suspension are mixed in proportion; S5: addition of growth regulators; The auxin and cytokinin are dissolved in the trace element solution according to the proportion, and then evenly mixed into the microbial mixture; S6: adsorption and drying; Spray the mixture onto the pretreated organic substrate to allow it to fully absorb, and then slowly dry it in a well-ventilated environment to avoid excessive temperature that may cause loss of microbial activity. S7: Finished product; The dried mixture is crushed into uniform particles or powder, and packed into light-proof sealed bags; S8: Storage and transportation; Store the finished product in a cool, dry place, avoid high temperature or humidity to maintain the activity of the bacteria. Try to avoid direct sunlight and high temperature conditions during transportation.

9. The microbial preparation for anti-transpiration of crops according to claim 1, characterized in that: In step S3, after humic acid or compost is uniformly mixed, the pH value is adjusted to 7.

10. Application of a microbial preparation for anti-transpiration of crops, characterized in that: Used for crop planting in arid and water-scarce areas.

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