Feed additive for promoting pesticide metabolism in animal body and preparation method thereof

Abstract

The invention discloses a feed additive for promoting pesticide metabolism in animal bodies and a preparation method of the feed additive, and belongs to the technical field of feed additives. Comprising the following raw materials in parts by weight: 22-30 parts of a mashed sweet potato fermentation product, 11-18 parts of a chrysanthemum straw extract, 8-15 parts of a goat milk heat treatment product, 10-20 parts of citrus peel, 5-11 parts of glycyrrhizic acid, 1-3 parts of pesticide degradation composite biological bacteria and 5-8 parts of a polyphenol compound. According to the mode, the feed additive prepared from the mashed sweet potato fermentation product, the chrysanthemum straw extract, the goat milk heat treatment product, the citrus peel, the glycyrrhizic acid, the pesticide degradation composite biological bacteria and the polyphenol compound can improve the metabolism result of pesticides in animal bodies, and meanwhile, the dosage of the feed additive is small.

Description

Technical Field

[0001] The invention relates to the technical field of feed additives, and in particular to a feed additive for promoting pesticide metabolism in animals and a preparation method thereof. Background Art

[0002] As people become more aware of food safety, the pesticide content in animals also needs to be strictly controlled. Since animal feed inevitably contains a certain amount of organic pesticides, if they accumulate in large quantities in the animal's body and are difficult to metabolize, it will not only endanger the health of the animal itself, but also pose a hidden danger to food safety.

[0003] Chinese invention patent CN110373367B discloses a plant lactobacillus with pesticide degradation activity, a preparation method and application thereof, which has the effect of degrading organophosphorus pesticides.

[0004] However, the detection method is: inoculate the strain into MRS medium containing omethoate, phorate and dimethoate, culture at 37℃ for 24h, and measure the concentrations of omethoate, phorate and dimethoate by gas chromatography-mass spectrometry. Since the strain is affected by the stomach environment and food ingredients after entering the stomach of the animal, it is difficult to accurately know the effect of the strain on the metabolism of pesticides in the animal. At the same time, it is directly used as a feed additive, and the dosage is large.

[0005] Based on this, the present invention designs a feed additive for promoting pesticide metabolism in animals and a preparation method thereof to solve the above problems. Summary of the invention

[0006] In view of the above-mentioned shortcomings of the prior art, the present invention provides a feed additive for promoting the metabolism of pesticides in animals and a preparation method thereof.

[0007] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0008] A feed additive for promoting pesticide metabolism in animals, comprising the following raw materials in parts by weight: 22-30 parts of sweet potato puree fermentation product, 11-18 parts of chrysanthemum straw extract, 8-15 parts of goat milk heat treatment product, 10-20 parts of citrus peel, 5-11 parts of glycyrrhizic acid, 1-3 parts of pesticide-degrading composite biological bacteria, and 5-8 parts of polyphenol compounds.

[0009] Furthermore, the pesticide-degrading composite biological bacteria include Bacillus cereus ZH-3, Staphylococcus aureus HP-S-01 and Pseudomonas sp. JD37.

[0010] Furthermore, the polyphenol compound is prepared by mixing caffeic acid, chlorogenic acid, sorghum shell polyphenols and mulberry polyphenols in a ratio of 1:1:2.3-5:0.3-1.

[0011] Furthermore, in the pesticide degradation composite biological bacteria, the number of viable Bacillus cereus ZH-3 is greater than 1×10 8 cfu / g, Staphylococcus aureus HP-S-01 viable count>2×10 8 cfu / g, Pseudomonas sp. JD37 viable count>5×10 8 cfu / g.

[0012] Furthermore, the preparation method of the sweet potato puree fermentation product is: the sweet potatoes are cooked and mashed into a puree, cooled to 36-37° C., inoculated with Lactobacillus helveticus_CICC 6064, the inoculation amount is 0.2-0.5 g / kg sweet potato puree, aerobic fermentation is carried out for 13117 hours, the supernatant is separated after sterilization, the pH is first adjusted to 7-7.3, and then the supernatant is irradiated with an electron beam of 6.3-7.5 kGy, and the sweet potato puree fermentation product is obtained after drying.

[0013] Furthermore, the chrysanthemum straw extract adopts a common alcohol extraction process, and the preparation method is: crush the chrysanthemum straw, add it into 4 to 5 times the volume of 70% ethanol solution and soak it for 15 to 24 hours, distill it under reduced pressure and recover ethanol, and then dry it to obtain the chrysanthemum straw extract.

[0014] Furthermore, the preparation method of the goat milk heat treatment product is: add 0.01 to 0.03 times the weight of physiological saline to the goat milk, mix well and then inject water vapor at 115 to 125°C, the water vapor flow rate is 5 to 8 m / s, the diameter of the water vapor pipe is 10 to 20 cm, the water vapor introduction time is 30 to 50 s, and the goat milk heat treatment product is obtained after drying.

[0015] In order to better achieve the purpose of the present invention, the present invention also provides a method for preparing a feed additive for promoting pesticide metabolism in animals, comprising the following steps:

[0016] Step 1, mixing a heat-treated goat milk product, citrus peel, polyphenol compounds, glycyrrhizic acid, a fermented sweet potato puree product, and a chrysanthemum straw extract;

[0017] Step 2: Add the pesticide-degrading composite biological bacteria and mix evenly at 32-35°C.

[0018] In order to better achieve the purpose of the present invention, the present invention also provides a feed additive for promoting pesticide metabolism in animals prepared according to the method.

[0019] In order to better achieve the purpose of the present invention, the present invention also provides an application of a feed additive in the preparation of a feed for promoting pesticide metabolism in animals. The feed additive is added to malt powder, ultrasonically dispersed for 2 minutes, and then added to animal feed, ultrasonically dispersed for 3 minutes. The mass ratio of the feed additive to the malt powder is 1:1; the mass ratio of the feed additive to the animal feed is 4:100.

[0020] Compared with the prior art, the present invention has the following beneficial effects: the feed additive prepared by the present invention using sweet potato puree fermentation products, chrysanthemum straw extracts, goat milk heat treatment products, citrus peels, glycyrrhizic acid, pesticide-degrading composite biological bacteria, and polyphenol compounds can improve the pesticide metabolism results in animals, and at the same time, the feed additive is used in a small amount. DETAILED DESCRIPTION

[0021] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described in combination with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0022] Embodiment 1: In some embodiments, a method for preparing a feed additive for promoting pesticide metabolism in an animal comprises the following steps:

[0023] Step 1, preparing the fermented sweet potato puree product: cooking the sweet potatoes and mashing them into a puree, cooling them to 36° C., inoculating Lactobacillus helveticus_CICC 6064 with an inoculation amount of 0.2 g / kg of the sweet potato puree, performing aerobic fermentation for 13 h, separating the supernatant after sterilization, first adjusting the pH to 7, then irradiating the supernatant with a 6.3 kGy electron beam, and drying to obtain the fermented sweet potato puree product.

[0024] Step 2: prepare chrysanthemum straw extract using a common alcohol extraction process. The preparation method is as follows: crush the chrysanthemum straw, add it to 4 times the volume of 70% ethanol solution and soak it for 15 hours, perform vacuum distillation and recover ethanol, and obtain the chrysanthemum straw extract after drying.

[0025] Step 3, preparing the heat-treated product of goat milk: adding 0.01 times the weight of physiological saline to the goat milk, mixing evenly and then injecting 115°C steam, the steam flow rate is 5m / s, the diameter of the steam pipe is 10cm, the steam introduction time is 30s, and the heat-treated product of goat milk is obtained after drying.

[0026] Step 4, weighing raw materials: 22 parts of sweet potato puree fermentation product, 11 parts of chrysanthemum straw extract, 8 parts of goat milk heat treatment product, 10 parts of citrus peel, 5 parts of glycyrrhizic acid, 1 part of pesticide degradation composite biological bacteria, and 5 parts of polyphenol compounds;

[0027] The polyphenol compound is prepared by mixing caffeic acid, chlorogenic acid, sorghum shell polyphenol and mulberry polyphenol in a ratio of 1:1:2.3:0.3; the pesticide degradation composite biological bacteria include Bacillus cereus ZH-3, Staphylococcus aureus HP-S-01 and Pseudomonas sp. JD37; in the pesticide degradation composite biological bacteria, the number of live bacteria of Bacillus cereus ZH-3 is greater than 1×10 8 cfu / g, Staphylococcus aureus HP-S-01 viable count>2× 10 8 cfu / g, Pseudomonas sp. JD37 viable count>5×10 8 cfu / g.

[0028] Step 5: Mix the heat-treated goat milk product, citrus peel, polyphenol compounds, glycyrrhizic acid, sweet potato puree fermentation product and chrysanthemum straw extract.

[0029] Step 6: Add the pesticide-degrading composite biological bacteria and mix evenly at 32°C.

[0030] Embodiment 2: In some embodiments, a method for preparing a feed additive for promoting pesticide metabolism in an animal comprises the following steps:

[0031] Step 1, preparing the fermented sweet potato puree product: cooking the sweet potatoes and mashing them into a puree, cooling them to 37° C., inoculating Lactobacillus helveticus_CICC 6064 with an inoculation amount of 0.5 g / kg of the sweet potato puree, performing aerobic fermentation for 17 h, sterilizing and separating the supernatant, first adjusting the pH to 7.3, and then irradiating the supernatant with a 7.5 kGy electron beam, and drying to obtain the fermented sweet potato puree product.

[0032] Step 2: prepare chrysanthemum straw extract using a common alcohol extraction process. The preparation method is as follows: crush the chrysanthemum straw, add it to 5 times the volume of 70% ethanol solution and soak it for 24 hours, distill it under reduced pressure and recover the ethanol, and then dry it to obtain the chrysanthemum straw extract.

[0033] Step 3, preparing the heat-treated product of goat milk: add 0.03 times the weight of physiological saline to the goat milk, mix well and then inject 125°C steam, the steam flow rate is 8m / s, the diameter of the steam pipe is 20cm, the steam introduction time is 50s, and the heat-treated product of goat milk is obtained after drying.

[0034] Step 4, weighing raw materials: 30 parts of sweet potato puree fermentation product, 18 parts of chrysanthemum straw extract, 15 parts of goat milk heat treatment product, 20 parts of citrus peel, 11 parts of glycyrrhizic acid, 3 parts of pesticide degradation composite biological bacteria, and 8 parts of polyphenol compounds;

[0035] The polyphenol compound is prepared by mixing caffeic acid, chlorogenic acid, sorghum shell polyphenol and mulberry polyphenol in a ratio of 1:1:5:1; the pesticide degradation composite biological bacteria include Bacillus cereus ZH-3, Staphylococcus aureus HP-S-01 and Pseudomonas sp. JD37; in the pesticide degradation composite biological bacteria, the number of live bacteria of Bacillus cereus ZH-3 is greater than 1×10 8 cfu / g, Staphylococcus aureus HP-S-01 viable count>2×10 8 cfu / g, Pseudomonas sp. JD37 viable count>5×10 8 cfu / g.

[0036] Step 5: Mix the heat-treated goat milk product, citrus peel, polyphenol compounds, glycyrrhizic acid, sweet potato puree fermentation product and chrysanthemum straw extract.

[0037] Step 6: Add the pesticide-degrading composite biological bacteria and mix evenly at 35°C.

[0038] Embodiment 3: In some embodiments, a method for preparing a feed additive for promoting pesticide metabolism in an animal comprises the following steps:

[0039] Step 1, preparing the fermented sweet potato puree product: cooking the sweet potatoes and mashing them into a puree, cooling them to 36.5° C., inoculating Lactobacillus helveticus_CICC 6064 with an inoculation amount of 0.3 g / kg of the sweet potato puree, performing aerobic fermentation for 15 h, separating the supernatant after sterilization, first adjusting the pH to 7.1, and then irradiating the supernatant with a 7 kGy electron beam, and drying to obtain the fermented sweet potato puree product.

[0040] Step 2: prepare chrysanthemum straw extract using a common alcohol extraction process. The preparation method is as follows: crush the chrysanthemum straw, add it to 4.2 times the volume of 70% ethanol solution and soak it for 20 hours, perform vacuum distillation and recover ethanol, and obtain the chrysanthemum straw extract after drying.

[0041] Step 3, preparing the heat-treated product of goat milk: add 0.02 times the weight of physiological saline to the goat milk, mix well and then inject 120°C steam, the steam flow rate is 6m / s, the diameter of the steam pipe is 15cm, the steam introduction time is 40s, and the heat-treated product of goat milk is obtained after drying.

[0042] Step 4, weighing raw materials: 25 parts of sweet potato puree fermentation product, 16 parts of chrysanthemum straw extract, 10 parts of goat milk heat treatment product, 12 parts of citrus peel, 7 parts of glycyrrhizic acid, 2 parts of pesticide degradation composite biological bacteria, and 6 parts of polyphenol compounds;

[0043] The polyphenol compound is prepared by mixing caffeic acid, chlorogenic acid, sorghum shell polyphenol and mulberry polyphenol in a ratio of 1:1:3:0.5; the pesticide degradation composite biological bacteria include Bacillus cereus ZH-3, Staphylococcus aureus HP-S-01 and Pseudomonas sp. JD37: in the pesticide degradation composite biological bacteria, the number of live bacteria of Bacillus cereus ZH-3 is greater than 1×10 8 cfu / g, Staphylococcus aureus HP-S-01 viable count>2×10 8 cfu / g, Pseudomonas sp. JD37 viable count>5×10 8 cfu / g.

[0044] Step 5: Mix the heat-treated goat milk product, citrus peel, polyphenol compounds, glycyrrhizic acid, sweet potato puree fermentation product and chrysanthemum straw extract.

[0045] Step 6: Add the pesticide-degrading composite biological bacteria and mix evenly at 34°C.

[0046] Example 4: In some embodiments, a method for preparing a feed additive for promoting pesticide metabolism in animals comprises the following steps:

[0047] Step 1, preparing the fermented sweet potato puree product: cooking the sweet potatoes and mashing them into a puree, cooling them to 36° C., inoculating Lactobacillus helveticus_CICC 6064 with an inoculation amount of 0.4 g / kg of the sweet potato puree, performing aerobic fermentation for 16 h, separating the supernatant after sterilization, first adjusting the pH to 7, then irradiating the supernatant with a 7 kGy electron beam, and drying to obtain the fermented sweet potato puree product.

[0048] Step 2: prepare chrysanthemum straw extract using a common alcohol extraction process. The preparation method is as follows: crush the chrysanthemum straw, add it to 4 times the volume of 70% ethanol solution and soak it for 20 hours, perform vacuum distillation and recover ethanol, and obtain the chrysanthemum straw extract after drying.

[0049] Step 3, preparing the heat-treated product of goat milk: adding 0.02 times the weight of physiological saline to the goat milk, mixing evenly and then flushing with 122°C steam, the steam flow rate is 6.5m / s, the diameter of the steam pipe is 18cm, the steam introduction time is 45s, and the heat-treated product of goat milk is obtained after drying.

[0050] Step 4, weighing raw materials: 28 parts of sweet potato puree fermentation product, 17 parts of chrysanthemum straw extract, 12 parts of goat milk heat treatment product, 14 parts of citrus peel, 10 parts of glycyrrhizic acid, 2 parts of pesticide degradation composite biological bacteria, and 7 parts of polyphenol compounds;

[0051] The polyphenol compound is prepared by mixing caffeic acid, chlorogenic acid, sorghum shell polyphenol and mulberry polyphenol in a ratio of 1:1:4:0.6; the pesticide degradation composite biological bacteria include Bacillus cereus ZH-3, Staphylococcus aureus HP-S-01 and Pseudomonas sp. JD37; in the pesticide degradation composite biological bacteria, the number of live bacteria of Bacillus cereus ZH-3 is greater than 1×10 8 cfu / g, Staphylococcus aureus HP-S-01 viable count>2× 10 8 cfu / g, Pseudomonas sp. JD37 viable count>5×10 8 cfu / g.

[0052] Step 5: Mix the heat-treated goat milk product, citrus peel, polyphenol compounds, glycyrrhizic acid, sweet potato puree fermentation product and chrysanthemum straw extract.

[0053] Step 6: Add the pesticide-degrading composite biological bacteria and mix evenly at 34°C.

[0054] Example 5: In some embodiments, a method for preparing a feed additive for promoting pesticide metabolism in an animal comprises the following steps:

[0055] Step 1, preparing the fermented sweet potato puree product: cooking the sweet potatoes and mashing them into a puree, cooling them to 36°C, inoculating Lactobacillus helveticus_CICC 6064 with an inoculation amount of 0.4 g / kg of sweet potato puree, performing aerobic fermentation for 14 h, separating the supernatant after sterilization, first adjusting the pH to 7.1, and then irradiating the supernatant with a 6.6 Gy electron beam, and drying to obtain the fermented sweet potato puree product.

[0056] Step 2: prepare chrysanthemum straw extract using a common alcohol extraction process. The preparation method is as follows: crush the chrysanthemum straw, add it to 4.3 times the volume of 70% ethanol solution and soak it for 20 hours, perform vacuum distillation and recover ethanol, and obtain the chrysanthemum straw extract after drying.

[0057] Step 3, preparing the heat-treated product of goat milk: adding 0.02 times the weight of physiological saline to the goat milk, mixing evenly and then injecting water vapor at 118°C, with a water vapor flow rate of 6m / s, a water vapor pipe diameter of 17cm, and a water vapor introduction time of 44s, and obtaining the heat-treated product of goat milk after drying.

[0058] Step 4, weighing raw materials: 26 parts of sweet potato puree fermentation product, 13 parts of chrysanthemum straw extract, 11 parts of goat milk heat treatment product, 15 parts of citrus peel, 7 parts of glycyrrhizic acid, 1.8 parts of pesticide degradation composite biological bacteria, and 6.8 parts of polyphenol compounds;

[0059] The polyphenol compound is prepared by mixing caffeic acid, chlorogenic acid, sorghum shell polyphenol and mulberry polyphenol in a ratio of 1:1:4.5:0.4; the pesticide degradation composite biological bacteria include Bacillus cereus ZH-3, Staphylococcus aureus HP-S-01 and Pseudomonas sp. JD37; in the pesticide degradation composite biological bacteria, the number of live bacteria of Bacillus cereus ZH-3 is greater than 1×10 8 cfu / g, Staphylococcus aureus HP-S-01 viable count>2× 10 8 cfu / g, Pseudomonas sp. JD37 viable count>5×10 8 cfu / g.

[0060] Step 5: Mix the heat-treated goat milk product, citrus peel, polyphenol compounds, glycyrrhizic acid, sweet potato puree fermentation product and chrysanthemum straw extract.

[0061] Step 6: Add the pesticide-degrading composite biological bacteria and mix evenly at 33°C.

[0062] Comparative Example 1: Different from Example 5, no heat-treated goat milk product was added.

[0063] A method for preparing a feed additive for promoting pesticide metabolism in animals comprises the following steps:

[0064] Step 1, preparing the fermented sweet potato puree product: cooking the sweet potatoes and mashing them into a puree, cooling them to 36°C, inoculating Lactobacillus helveticus_CICC 6064 with an inoculation amount of 0.4 g / kg of sweet potato puree, performing aerobic fermentation for 14 h, separating the supernatant after sterilization, first adjusting the pH to 7.1, and then irradiating the supernatant with a 6.6 Gy electron beam, and drying to obtain the fermented sweet potato puree product.

[0065] Step 2: prepare chrysanthemum straw extract using a common alcohol extraction process. The preparation method is as follows: crush the chrysanthemum straw, add it to 4.3 times the volume of 70% ethanol solution and soak it for 20 hours, perform vacuum distillation and recover ethanol, and obtain the chrysanthemum straw extract after drying.

[0066] Step 3, weighing raw materials: 26 parts of sweet potato puree fermentation product, 13 parts of chrysanthemum straw extract, 15 parts of citrus peel, 7 parts of glycyrrhizic acid, 1.8 parts of pesticide degradation composite biological bacteria, and 6.8 parts of polyphenol compounds;

[0067] The polyphenol compound is prepared by mixing caffeic acid, chlorogenic acid, sorghum shell polyphenol and mulberry polyphenol in a ratio of 1:1:4.5:0.4; the pesticide degradation composite biological bacteria include Bacillus cereus ZH-3, Staphylococcus aureus HP-S-01 and Pseudomonas sp. JD37: in the pesticide degradation composite biological bacteria, the number of live bacteria of Bacillus cereus ZH-3 is greater than 1×10 8 cfu / g, Staphylococcus aureus HP-S-01 viable count>2×10 8 cfu / g, Pseudomonas sp. JD37 viable count>5×10 8 cfu / g.

[0068] Step 4: Mix the citrus peel, polyphenol compounds, glycyrrhizic acid, sweet potato puree fermentation product and chrysanthemum straw extract.

[0069] Step 5: Add the pesticide-degrading composite biological bacteria and mix evenly at 33°C.

[0070] Comparative Example 2: Different from Example 5, no chrysanthemum straw extract was added.

[0071] A method for preparing a feed additive for promoting pesticide metabolism in animals comprises the following steps:

[0072] Step 1, preparing the fermented sweet potato puree product: cooking the sweet potatoes and mashing them into a puree, cooling them to 36°C, inoculating Lactobacillus helveticus_CICC 6064 with an inoculation amount of 0.4 g / kg of sweet potato puree, performing aerobic fermentation for 14 h, separating the supernatant after sterilization, first adjusting the pH to 7.1, and then irradiating the supernatant with a 6.6 Gy electron beam, and drying to obtain the fermented sweet potato puree product.

[0073] Step 2, preparing the heat-treated product of goat milk: adding 0.02 times the weight of physiological saline to the goat milk, mixing evenly and then injecting 118°C steam, the steam flow rate is 6m / s, the diameter of the steam pipe is 17cm, the steam introduction time is 44s, and the heat-treated product of goat milk is obtained after drying.

[0074] Step 3, weighing raw materials: 26 parts of sweet potato puree fermentation product, 11 parts of goat milk heat treatment product, 15 parts of citrus peel, 7 parts of glycyrrhizic acid, 1.8 parts of pesticide degradation composite biological bacteria, and 6.8 parts of polyphenol compounds;

[0075] The polyphenol compound is prepared by mixing caffeic acid, chlorogenic acid, sorghum shell polyphenol and mulberry polyphenol in a ratio of 1:1:4.5:0.4; the pesticide degradation composite biological bacteria include Bacillus cereus ZH-3, Staphylococcus aureus HP-S-01 and Pseudomonas sp. JD37; in the pesticide degradation composite biological bacteria, the number of live bacteria of Bacillus cereus ZH-3 is greater than 1×10 8 cfu / g, Staphylococcus aureus HP-S-01 viable count>2×10 8 cfu / g, Pseudomonas sp. JD37 viable count>5×10 8 cfu / g.

[0076] Step 4: Mix the heat-treated goat milk product, citrus peel, polyphenol compounds, glycyrrhizic acid and sweet potato puree fermentation product.

[0077] Step 5: Add the pesticide-degrading composite biological bacteria and mix evenly at 33°C.

[0078] Comparative Example 3: Different from Example 5, no electron beam irradiation treatment is performed in the preparation of the sweet potato puree fermentation product.

[0079] Test example: 60 SPF grade SD male rats with an average body weight of 250g were selected and randomly divided into a control group (comparative example 1, 2, 3 groups) and a treatment group (example 1, 2, 5 groups), with 10 rats in each group. The rats in each group were grown at room temperature and fed and watered freely for 2 weeks in an aseptic manner. The experiment was conducted in accordance with the Regulations on the Administration of Experimental Animals.

[0080] Preparation of pesticide-containing feed: Add imidacloprid to the feed at a rate of 10 mg / kg feed.

[0081] The comparative examples 1, 2 and 3 groups consumed 35 g of pesticide-containing feed with feed additives and 50 ml of water every day; specifically, the feed additives prepared in comparative examples 1, 2 and 3 were added to malt powder, ultrasonically dispersed for 2 minutes, and then added to the pesticide-containing feed, ultrasonically dispersed for 3 minutes, the mass ratio of the feed additive to the malt powder was 1:1; the mass ratio of the feed additive to the pesticide-containing feed was 4:100.

[0082] The groups of Examples 1, 2, and 5 consumed 35 g of feed with feed additives and 50 ml of water every day: specifically, the feed additives prepared in Examples 1, 2, and 5 were added to malt powder, ultrasonically dispersed for 2 minutes, and then added to the pesticide-containing feed, ultrasonically dispersed for 3 minutes. The mass ratio of the feed additive to the malt powder was 1:1; the mass ratio of the feed additive to the pesticide-containing feed was 4:100.

[0083] After 2 weeks, the pesticide residues in the rats of each group were detected, and the degradation rates of pesticides in the serum and urine of the rats were calculated. The results are shown in Table 1.

[0084] Table 1 Pesticide degradation rate results

[0085] project Example 1 Example 2 Example 5 Comparative Example 1 Comparative Example 2 Comparative Example 3 Degradation rate 71.0％ 75.6％ 73.8％ 60.3％ 57.9％ 66.8％

[0086] It can be seen that the feed additive prepared by the present invention using sweet potato puree fermentation products, chrysanthemum straw extracts, goat milk heat treatment products, citrus peels, glycyrrhizic acid, pesticide-degrading composite biological bacteria, and polyphenol compounds can improve the pesticide metabolism results in animals, and at the same time, the amount of it used as a feed additive is small.

[0087] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. Such modifications or replacements will not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A feed additive for promoting pesticide metabolism in animals, characterized in that: The invention comprises the following raw materials in parts by weight: 22-30 parts of sweet potato puree fermentation product, 11-18 parts of chrysanthemum straw extract, 8-15 parts of goat milk heat treatment product, 10-20 parts of citrus peel, 5-11 parts of glycyrrhizic acid, 1-3 parts of pesticide degradation composite biological bacteria and 5-8 parts of polyphenol compounds.

2. The feed additive for promoting pesticide metabolism in animals according to claim 1, characterized in that: The pesticide degradation complex includes Bacillus cereus, Staphylococcus aureus and Pseudomonas.

3. The feed additive for promoting pesticide metabolism in animals according to claim 2, characterized in that: The polyphenol compound is prepared by mixing caffeic acid, chlorogenic acid, sorghum shell polyphenol and mulberry polyphenol in a ratio of 1:1:2.3-5:0.3-1.

4. The feed additive for promoting pesticide metabolism in animals according to claim 3, characterized in that: The number of viable Bacillus cereus in the pesticide degradation composite biological bacteria is greater than 1×10 8 cfu / g, viable Staphylococcus aureus count>2×10 8 cfu / g, Pseudomonas viable count>5×10 8 cfu / g.

5. The feed additive for promoting pesticide metabolism in animals according to claim 4, characterized in that: The preparation method of the sweet potato puree fermentation product comprises the following steps: cooking sweet potatoes and beating them into a puree, cooling them to 36-37° C., inoculating Lactobacillus helveticus with an inoculation amount of 0.2-0.5 g / kg of sweet potato puree, performing aerobic fermentation for 13-17 hours, sterilizing and separating the supernatant, first adjusting the pH to 7-7.3, then irradiating the supernatant with an electron beam of 6.3-7.5 kGy, and drying to obtain the sweet potato puree fermentation product.

6. The feed additive for promoting pesticide metabolism in animals according to claim 5, characterized in that: The chrysanthemum straw extract adopts a common alcohol extraction process, and the preparation method is: crushing the chrysanthemum straw, adding it into 4 to 5 times the volume of 70% ethanol solution and soaking it for 15 to 24 hours, distilling it under reduced pressure and recovering ethanol, and drying it to obtain the chrysanthemum straw extract.

7. The feed additive for promoting pesticide metabolism in animals according to claim 6, characterized in that: The preparation method of the goat milk heat treatment product is as follows: 0.01 to 0.03 times the weight of physiological saline is added to the goat milk, and after uniform mixing, water vapor at 115 to 125° C. is injected, the water vapor flow rate is 5 to 8 m / s, the diameter of the water vapor pipe is 10 to 20 cm, the water vapor injection time is 30 to 50 s, and the goat milk heat treatment product is obtained after drying.

8. A method for preparing a feed additive for promoting pesticide metabolism in animals according to claim 7, characterized in that: The following steps are involved: Step 1, mixing a heat-treated goat milk product, citrus peel, polyphenol compounds, glycyrrhizic acid, a fermented sweet potato puree product, and a chrysanthemum straw extract; Step 2: Add the pesticide-degrading composite biological bacteria and mix evenly at 32-35°C.

9. A feed additive for promoting pesticide metabolism in animals prepared according to the method of claim 8.

10. Use of the feed additive according to claim 9 in preparing feed for promoting pesticide metabolism in animals, characterized in that: The feed additive was added to malt powder, subjected to ultrasonic dispersion for 2 minutes, and then added to animal feed, subjected to ultrasonic dispersion for 3 minutes. The mass ratio of the feed additive to the malt powder was 1:1; the mass ratio of the feed additive to the animal feed was 4:100.