Full-automatic methylated gene preparation workstation and working method thereof

Abstract

The invention discloses a full-automatic methylated gene preparation work station and a working method thereof, the preparation work station comprises: a frame body comprising a bottom plate and a vertical frame connected with the bottom plate; the uniform shaking puncture position is mounted on the bottom plate, is connected with the vertical frame, and is used for uniformly shaking a sample in the collecting tube and discharging the sample into a storage vessel; the liquid moving level is connected with the vertical frame and used for sucking the sample in the storage vessel and moving the sample into a test tube placed in the workbench, and the sample comprises excrement; the workbench is mounted on the bottom plate and is used for placing test tubes; and the purification position is connected with the vertical frame and is used for purifying the sample placed in the test tube. According to the invention, the sample in the collection tube is shaken up by designing the shaking-up puncture position, the sample is discharged into the storage vessel, the sample in the storage vessel is sucked through the liquid transfer position and moved into the test tube placed in the workbench, and finally, the purification work of the test tube sample is completed by the purification position, so that the preparation is completed.

Description

Technical Field

[0001] The invention relates to the field of gene preparation, and in particular to a fully automatic methylation gene preparation workstation and a working method thereof. Background Art

[0002] A methylation gene preparation workstation usually refers to laboratory equipment and operating platforms used for DNA methylation analysis in molecular biology research.

[0003] DNA methylation is an epigenetic modification that involves the addition of methyl groups to cytosine residues in DNA molecules. This modification can regulate gene expression and plays a key role in many biological processes, such as embryonic development, X chromosome inactivation, gene imprinting, and cancer.

[0004] At present, when preparing samples, the methylation gene preparation workstation requires manual intervention in the process of shaking the samples to filtering and purifying, which belongs to semi-automatic preparation. During the manual intervention process, if some personnel do not pay attention to the operating specifications, it is easy to affect the preparation effect;

[0005] Therefore, it is necessary to propose a fully automatic methylation gene preparation workstation to solve this problem. After the workstation starts working, there is no need to interfere with the workstation to achieve sample input and gene output. Summary of the invention

[0006] Purpose of the invention: To provide a fully automatic methylation gene preparation workstation and its working method to solve the above problems existing in the prior art.

[0007] Technical solution: A fully automatic methylation gene preparation workstation, including:

[0008] The frame body comprises a bottom plate and a vertical frame connected to the bottom plate;

[0009] A shaking and piercing position, mounted on the bottom plate and connected to the stand, for shaking the sample in the collection tube and discharging the sample into a storage container;

[0010] A transfer station, connected to the stand, for sucking the sample in the storage container and moving it to a test tube placed on the workbench, wherein the sample includes feces;

[0011] A workbench, mounted on the bottom plate, for placing test tubes;

[0012] The purification station is connected to the stand and is used for purifying the sample placed in the test tube.

[0013] The present invention shakes the sample in the collection tube by designing a shaking and piercing position, and discharges the sample into a storage container, absorbs the sample in the storage container through a liquid transfer position, and moves it to a test tube placed on a workbench, and finally purifies the test tube sample by a purification position, thereby completing the preparation;

[0014] When the sample is shaken, the switching unit drives the storage vessel and the shaking seat to switch between each other. When shaking, the shaking seat contacts the collection tube, and the shaking part drives the shaking claw to contact the collection tube cover and rotate to complete the shaking. When the shaken sample needs to be discharged, the storage vessel is switched and the one-way valve of the collection tube is pressed against the shaking rod. At the same time, the piston body is driven to move to quickly discharge the shaken sample into the storage vessel. The sample in the storage vessel is then moved into the test tube by the pipetting position, and the purification position completes the purification through the cooperation of the purification magnetic rod and the magnetic rod sleeve. After purification, the magnetic rod sleeve is automatically replaced. After the workstation starts working, the whole process does not require manual intervention. Compared with the traditional semi-automatic workstation with manual intervention, the present application only needs to put the collection tube into the tube rack before the workstation works, and push the tube rack into the low-level rack to complete the manual working stage. The subsequent preparation work is completed by the workstation without manual intervention, realizing the work of sample in and gene out.

[0015] In a further embodiment, the stand is provided with a driving unit, and the driving unit comprises:

[0016] A connecting plate connected to the stand;

[0017] The driving member is designed in two groups and is installed on the connecting plate. Each group includes driving wheels arranged at both ends of the connecting plate, a driving belt sleeved on the driving wheels, and a driving motor connected to the connecting plate and having an output end connected to any one group of driving wheels.

[0018] The inner side of the stand is provided with lateral slide rails, and the top is provided with two groups of top slide rails.

[0019] In a further embodiment, the transfer station comprises:

[0020] The liquid transfer lateral unit comprises a liquid transfer lateral linear module connected to the driving member and adapted to the top slide rail and the lateral slide rail and used to drive the liquid transfer vertical unit to move laterally;

[0021] The pipetting transverse linear module comprises a pipetting transverse frame, a pipetting slide rail mounted on the side of the pipetting transverse frame, two groups of pipetting transverse wheels arranged on the pipetting transverse frame, a pipetting transverse belt sleeved between the two groups of pipetting transverse wheels, and a pipetting transverse motor connected to the pipetting transverse frame and having an output end connected to any one group of pipetting transverse wheels;

[0022] The pipetting horizontal frame has a pipetting side slider adapted to the lateral slide rail at one end, and a pipetting bottom slider adapted to the top slide rail at the bottom of the other end;

[0023] The pipetting horizontal frame is connected to a driving belt in a group of driving members;

[0024] A pipetting vertical unit, comprising a pipetting vertical lifting module connected to the pipetting horizontal linear module and used to drive the pipette to perform lifting motion;

[0025] A pipette is connected to the pipetting vertical unit.

[0026] The pipetting vertical lifting module includes a pipetting vertical frame connected to the pipetting transverse belt, a pipetting vertical motor installed on the top of the pipetting vertical frame, a pipetting vertical screw connected to the output end of the pipetting vertical motor, and a pipetting vertical sliding sleeve sleeved on the pipetting vertical screw;

[0027] The pipette is connected to the pipetting vertical sliding sleeve;

[0028] The pipetting vertical frame is also provided with a pipetting vertical slide rail, and the pipetting vertical slide rail is adapted to be provided with a pipetting vertical slide block connected to the pipetting vertical slide sleeve;

[0029] The back of the vertical pipetting frame is provided with a pipetting slide block matched with the pipetting slide rail.

[0030] By designing the transfer position, the sample in the storage container is aspirated and moved to the test tube on the workbench to complete the transfer process.

[0031] In a further embodiment, the purification site comprises:

[0032] A purification horizontal unit, including a purification horizontal moving module for driving the purification vertical unit to move horizontally;

[0033] The purification transverse moving module comprises a purification transverse frame, a purification transverse slide rail installed on the side of the purification transverse frame, two groups of purification transverse wheels installed on the top of the purification transverse frame, a purification transverse belt sleeved on the two groups of purification transverse wheels, and a purification transverse motor connected to the purification transverse frame and having an output end connected to any one group of purification transverse wheels;

[0034] One end of the purification transverse frame is provided with a purification side slider adapted to the lateral slide rail, and the bottom of the other end is provided with a purification bottom slider adapted to the top slide rail;

[0035] The purification transverse frame is connected to a driving belt in another set of driving members;

[0036] A purification vertical unit, comprising a secondary lifting module connected to the purification horizontal moving module for driving the purification magnetic rod in the purification unit to move up and down and separate the magnetic rod sleeve and the magnetic rod buckle;

[0037] The secondary lifting module includes a purification vertical slide connected to the purification horizontal belt, a purification first-level motor installed on the top of the purification vertical slide, a purification first-level lead screw connected to the output end of the purification first-level motor (which can be coaxially connected, or gear staggered meshing connection, or belt drive connection), a purification first-level frame sleeved on the purification first-level lead screw, a purification second-level motor installed on the top of the purification first-level frame, and a purification second-level lead screw connected to the output end of the purification second-level motor.

[0038] The back of the purification vertical slide is provided with a purification horizontal slide block adapted to the purification horizontal slide rail, and the front is provided with a purification primary slide rail, and the purification primary slide rail is adapted to be provided with a purification primary slide block connected to the purification primary rack;

[0039] The front of the purification primary rack is equipped with a purification secondary slide rail, and the purification secondary slide rail is equipped with a purification secondary slider;

[0040] The purification unit comprises a purification fixing plate fixedly connected to the secondary lifting module, and a purification secondary frame sleeved on the secondary lifting module and lifting and moving along the secondary lifting module;

[0041] The purification secondary rack is connected to the purification secondary slider;

[0042] The purification secondary frame sleeve is arranged on the purification secondary screw rod;

[0043] The purification fixing plate is arranged at the bottom of the purification primary frame, two groups of purification rebound parts are arranged on the purification fixing plate, and a plurality of magnetic rod buckles with through cavities are embedded inside the purification fixing plate;

[0044] A purification rebound plate connected to two groups of purification rebound members is provided below the purification fixed plate;

[0045] The purification secondary rack is equipped with multiple groups of purification magnetic rods, and the purification magnetic rods are adapted to the through cavity;

[0046] The purification rebound member comprises a cavity mounted on the purification fixing plate, a rod body penetrating the cavity and passing through the purification fixing plate and connected with the purification rebound plate, a stopper sleeved on the rod body and located in the cavity, and a return spring sleeved on the rod body and located between the stopper and the bottom of the cavity;

[0047] When the purification secondary frame is lowered to a low position, it abuts against the rod body in the purification rebound member, and drives the purification rebound plate to move, separating the magnetic rod sleeve and the magnetic rod buckle;

[0048] By designing the purification position, the purification work of the sample is completed. After the purification work is completed, the purification secondary frame is lowered to abut against the rod body in the purification rebound part, and the purification rebound plate is driven to move, and the magnetic rod cover is knocked off. The purified impurities are all attached to the magnetic rod cover. At this time, the magnetic rod cover is knocked off, so that the purification magnetic rod can repeatedly complete the purification work of the sample.

[0049] In a further embodiment, the shaking of the puncture site comprises:

[0050] A switch piercing portion is mounted on the bottom plate;

[0051] A low-level frame is installed on the bottom plate and is located above the switching piercing portion. A collection chute and a collection through slot are provided on the low-level frame. A first barcode scanner is provided on the side of the low-level frame.

[0052] A collecting part, arranged on the low-position frame and adapted to the collecting chute;

[0053] A high-position frame, located above the collecting part and connected to the vertical frame;

[0054] The shaking part is connected with the high-position frame and is located above the collecting part.

[0055] In a further embodiment, the switching piercing portion comprises:

[0056] The switching unit includes a switching movement driving member and a switching lifting driving member installed on the bottom plate and used for driving the piercing unit to move and lift;

[0057] The switching movement driving member includes a switching movement motor and a switching movement wheel seat installed on the bottom plate, a switching movement wheel 1 arranged on the switching movement wheel seat, a switching movement wheel 2 arranged at the output end of the switching movement motor, and a switching movement belt sleeved on the switching movement wheel 1 and the switching movement wheel 2;

[0058] The bottom plate is also equipped with a switching movable slide rail, on which a switching movable slider is adapted, and on which a switching movable slide plate connected to one side of the switching movable belt is installed;

[0059] The switching lifting drive member includes a switching frame connected to the switching moving slide plate, a switching lifting slide rail installed on the side of the switching frame, a switching lifting slider adapted to the switching lifting slide rail, a switching lifting plate connected to the switching lifting slider, a switching lifting motor connected to the switching frame, and a switching lifting screw connected to the output end of the switching lifting motor;

[0060] By designing a switching unit, the switching between the shaking seat and the storage container is completed. When shaking is required, the shaking seat is switched, and when the sample needs to be discharged, the storage container is switched;

[0061] The piercing unit comprises a piercing plate connected to the switching lifting plate and sleeved on the switching lifting screw rod, a storage chute provided on the piercing plate, a vessel rack adapted to the storage chute, a plurality of storage vessels placed in the vessel rack, and a plurality of shaking seats provided on the piercing plate;

[0062] The shaking seat comprises a seat rod connected to the piercing plate, and a seat body sleeved on the seat rod, and the seat body rotates along the seat rod.

[0063] In a further embodiment, the collection part includes a tube rack adapted to the collection chute, and a plurality of groups of collection tubes arranged in the tube rack;

[0064] The collection tube comprises:

[0065] A tube body, wherein the outer side of the top of the tube body is provided with a thread, and the inside of the tube body is provided with an adaptable inner cylinder;

[0066] A cover body, adapted to the thread, disposed on the top of the tube body, and having an extrusion through hole on the top of the cover body;

[0067] The piston cylinder comprises a cylinder body arranged in the tube body, and a limiting body connected to the cylinder body, abutting against the top of the tube body and located between the cover body and the tube body;

[0068] A piston body, disposed in the cylinder body and adapted to the cylinder body;

[0069] A filter cartridge is disposed in the tube body and below the cartridge body;

[0070] An adapter frame is arranged between the adapter inner cylinder and the filter cylinder, and a bayonet is provided on the adapter frame;

[0071] A one-way valve adapted to the adapting inner cylinder and connected to the adapting frame, comprising a connecting buckle adapted to the bayonet, a valve body connected to the connecting buckle and located in the adapting inner cylinder, and a force-bearing part arranged at the bottom of the valve body and located outside the adapting inner cylinder;

[0072] The valve body is provided with a discharge port, and a discharge passage connected with the discharge port and extending to the outside of the valve body;

[0073] The seat body is adapted to the tube body, and the inlet end of the storage container is adapted to the force-bearing part;

[0074] The bottom of the pipe rack is provided with a through groove matched with the shaking seat.

[0075] By designing a collection tube with a one-way valve and cooperating with a storage container, the sample can be automatically discharged. At the same time, a piston body is designed, which cooperates with a shaking rod that can be raised and lowered. The sample can be discharged quickly by pressing down and up. With this design, even if the sample is relatively viscous, it can be discharged smoothly.

[0076] In a further embodiment, the shaking unit comprises:

[0077] A shaking and lifting part, used for driving the shaking driving part and the shaking unit to lift, comprises a shaking and lifting motor connected to the high-position frame, a shaking and lifting screw connected to the output end of the shaking and lifting motor, and a shaking and lifting frame sleeved on the shaking and lifting screw;

[0078] A shaking driving unit is used to drive the shaking unit to complete the shaking of the sample, including a shaking driving motor connected to the shaking lifting frame, a shaking driving gear sleeved on the output end of the shaking driving motor, and a shaking output gear meshed with the shaking driving gear;

[0079] The shaking unit comprises a plurality of shaking transmission parts and shaking grabbing parts which are staggered and meshed with each other;

[0080] Each set of shaking transmission parts includes a transmission rod and a transmission gear sleeved on the transmission rod, and any set of transmission gears is meshed with the shaking output gear;

[0081] Each set of shaking grabbing members comprises a shaking rod, a shaking input gear sleeved on the shaking rod and meshed with the transmission gear, and a shaking claw arranged at the bottom of the shaking rod and adapted to the cover body;

[0082] The transmission rod and the shaking rod are both sleeved with a shaking bearing connected to the shaking lifting frame;

[0083] The shaking rod is provided with a shaking slot, and the shaking claw is provided with a shaking limit pin adapted to the shaking slot;

[0084] A shaking spring is sleeved between the shaking bearing of the shaking rod and the shaking limit pin;

[0085] A shaking connecting frame is connected between the high-position frame and the low-position frame, a shaking slide rail is provided on the shaking connecting frame, and a shaking sliding block connected with the shaking lifting frame is provided on the shaking slide rail.

[0086] Through the design of the shaking slide, the remote control rod can be raised and lowered, thereby driving the piston body to move, and the shaking claw is reset by the shaking spring to avoid delaying the next shaking work of the shaking claw.

[0087] In a further embodiment, the workbench comprises:

[0088] The storage part is arranged on the base, including a pull-out track installed on the bottom plate, a working storage rack arranged on the pull-out track and adapted to the pull-out track, the working storage rack is provided with a test tube, a waste box, and a magnetic rod sleeve, and a temperature control module is arranged inside the storage rack to control the temperature of the test tube. The temperature control module belongs to the prior art and can be formed by a heat exchanger of a heating module and a refrigeration module;

[0089] The code scanning part is arranged on the side of the working storage rack, and includes a working rack installed on the bottom plate, and a code scanning member arranged on the working rack for driving the code scanner to move along the working rack.

[0090] The code scanning component includes a working slide rail arranged on the working frame, two sets of working wheels, a working belt sleeved on the two sets of working wheels, and a working motor connected to any one set of working wheels;

[0091] A working slide block is provided on the working slide rail, and a second code scanner is installed on the working slide block.

[0092] A working method of a fully automatic methylation gene preparation workstation, comprising:

[0093] Step 1: Place the collection tube containing the test sample into the tube rack, and slide the tube rack into the low rack through the collection chute;

[0094] Step 2: Switch the moving drive member and the lifting drive member to drive the shaking seat to move to the bottom of the collection tube and press against the collection tube;

[0095] Step 3, the shaking lifting part drives the shaking driving part to complete the grabbing of the collection tube, and the shaking driving part completes the shaking work;

[0096] The shaking and lifting part drives the shaking and lifting screw rod to move through the shaking and lifting motor, drives the shaking and lifting frame to move up and down along the shaking slide rail, and drives the shaking claw of the shaking driving part to contact the cover of the collection tube;

[0097] The shaking drive unit drives the shaking drive gear to rotate through the shaking drive motor, drives the shaking output gear to rotate, drives the transmission gear to rotate, drives the shaking input gear to rotate, drives the shaking rod to rotate, drives the shaking claw to rotate, drives the collection tube to rotate, and completes the sample shaking work;

[0098] Step 4: After the sample is shaken, the moving drive member and the lifting drive member are switched to drive the shaking seat to separate from the bottom of the collection tube, and the storage container is driven to move to the bottom of the collection tube;

[0099] Step 5, after the storage vessel is switched, the switching moving drive member moves, the storage vessel abuts against the one-way valve, and continues to move upward. At this time, the storage vessel abuts against the force-bearing part to drive the valve body to rise. At this time, the discharge port contacts the sample stored inside the tube body, and the sample is filtered through the filter cartridge, through the discharge port and the discharge channel, and discharged into the storage vessel. When the sample is discharged, the shaking and lifting motor drives the shaking and lifting screw rod to move, and drives the shaking and lifting frame to continue to move downward along the shaking slide rail, so that the shaking rod drives the piston body to descend in the piston cylinder through the extrusion through hole, thereby accelerating the discharge speed of the sample;

[0100] Step 6: After the sample is discharged, the liquid transfer position completes the liquid transfer work, and the storage container is driven to move to a predetermined position by switching the moving drive member and the lifting drive member, and the sample is moved to the test tube on the workbench by the liquid transfer position;

[0101] When the switching moving driving member moves, the switching moving motor drives the switching moving wheel 2 to move, and drives the switching moving belt to move, and then drives the switching moving slide plate to slide along the switching moving slide rail, so as to complete the lifting and lowering movement of the switching lifting driving member;

[0102] When the switching lifting drive member moves, the switching lifting motor drives the piercing plate to rise along the switching lifting slide rail, thereby completing the position adjustment and height adjustment of the shaking seat and the storage container;

[0103] During pipetting, the pipette is driven to move back and forth between the storage vessel and the test tube by the transverse pipetting unit and the vertical pipetting unit to move the sample into the test tube;

[0104] The transverse unit of the liquid transfer is driven by the transverse motor of the liquid transfer to move the transverse wheel of the liquid transfer, drive the transverse belt of the liquid transfer to move, drive the vertical frame of the liquid transfer to move along the liquid transfer slide rail, and complete the transverse movement of the vertical unit of the liquid transfer;

[0105] The vertical transfer unit is driven by the vertical transfer motor to rotate the vertical transfer screw, which drives the vertical transfer sleeve to move along the vertical transfer rail, thereby driving the pipette to work;

[0106] Step 7: The purification of the sample in the test tube is completed through the purification position. The purification horizontal unit and the purification vertical unit cooperate to drive the magnetic bar buckle in the purification unit to engage with the magnetic bar sleeve placed on the workbench, and drive the purification magnetic bar to extend into the magnetic bar sleeve, and move the magnetic bar sleeve into the test tube to complete the purification of the test tube sample;

[0107] After the sample is purified, the purification secondary rack is driven to continue to descend through the purification vertical unit until it contacts the rod in the purification rebound member, and drives the purification rebound plate to move downward, separates the magnetic rod sleeve and the magnetic rod buckle, and completes the preparation. During separation, the magnetic rod sleeve is moved to the top of the waste box;

[0108] The purification horizontal unit is driven by the purification horizontal motor to move the purification horizontal wheel, drive the purification horizontal belt to move, drive the purification vertical slide to move along the purification horizontal slide rail, and complete the movement of the purification vertical unit;

[0109] The purification vertical unit is driven by the purification first-level motor to drive the purification first-level screw to rotate, driving the purification first-level frame to move up and down, and then the purification second-level motor drives the purification second-level screw to rotate, driving the purification second-level frame to move up and down.

[0110] Beneficial effects: The present invention discloses a fully automatic methylation gene preparation workstation and a working method thereof. The present invention shakes the sample in the collection tube by designing a shaking and piercing position, discharges the sample into a storage container, absorbs the sample in the storage container by a liquid transfer position, and moves it to a test tube placed on a workbench, and finally purifies the test tube sample by a purification position, thereby completing the preparation;

[0111] When the sample is shaken, the switching unit drives the storage vessel and the shaking seat to switch between each other. When shaking, the shaking seat contacts the collection tube, and the shaking part drives the shaking claw to contact the collection tube cover and rotate to complete the shaking. When the shaken sample needs to be discharged, the storage vessel is switched and the one-way valve of the collection tube is pressed against the shaking rod. At the same time, the piston body is driven to move to quickly discharge the shaken sample into the storage vessel, and then the sample in the storage vessel is moved into the test tube by the pipetting position, and then the purification position completes the purification work through the cooperation of the purification magnetic rod and the magnetic rod sleeve. At the same time, the magnetic rod sleeve can be automatically replaced. After the workstation starts working, the whole process does not require manual intervention. Compared with the traditional semi-automatic workstation with manual intervention, the present application only needs to put the collection tube into the tube rack before the workstation works, and push the tube rack into the low-level rack to complete the manual working stage. The subsequent preparation work is completed by the workstation without manual intervention, realizing the work of sample in and gene out. BRIEF DESCRIPTION OF THE DRAWINGS

[0112] Figure 1 It is a structural schematic diagram of the present invention.

[0113] Figure 2 It is a schematic diagram of the structure of the liquid transfer position and the purification position of the present invention.

[0114] Figure 3 It is a schematic diagram of the frame structure of the present invention.

[0115] Figure 4 It is a schematic diagram of the liquid transfer position structure of the present invention.

[0116] Figure 5 It is a schematic diagram of the purification site structure of the present invention.

[0117] Figure 6 It is a schematic diagram of the purification vertical unit structure of the present invention.

[0118] Figure 7 It is a schematic diagram of the structure of the purification resilient member of the present invention.

[0119] Figure 8 It is a schematic diagram of the structure of the shaking and piercing position of the present invention.

[0120] Fig. 9 It is a schematic diagram of the structure of the switching piercing portion of the present invention.

[0121] Fig.10 It is a schematic diagram of the split structure of the switchable piercing portion of the present invention.

[0122] Fig.11 It is a schematic diagram of the structure of the collection part of the present invention.

[0123] Fig.12 It is a schematic structural diagram of a shaking portion of the present invention.

[0124] Fig.13 It is a structural schematic diagram of the shaking drive unit of the present invention.

[0125] Fig.14 It is a schematic diagram of the structure of the shaking unit of the present invention.

[0126] Fig.15 It is a schematic diagram of the structure of the collection tube of the present invention.

[0127] Fig.16 It is a schematic diagram of the tube structure of the present invention.

[0128] Fig.17 It is a schematic diagram of the one-way valve structure of the present invention.

[0129] Fig.18 It is a schematic diagram of the workbench structure of the present invention.

[0130] The accompanying drawings are marked as follows:

[0131] 1. frame; 11. bottom plate; 12. vertical frame; 122. lateral slide rail; 123. top slide rail; 124. driving unit; 124A. driving wheel; 124B. driving belt; 124C. connecting plate;

[0132] 2. Shake the piercing position; 21. Low position rack;

[0133] 22, switch the piercing part; 221, switch the unit; 221A, switch the moving slide rail; 221B, switch the moving slide plate; 221C, switch the frame; 221D, switch the lifting slide rail; 221E, switch the lifting plate; 221F, switch the lifting motor; 221G, switch the moving motor; 221H, switch the moving wheel seat; 221I, switch the moving belt;

[0134] 222, piercing unit; 222A, piercing plate; 222B, vessel rack; 222C, vessel storage; 222D, shaking seat;

[0135] 23, collection part; 231, pipe rack; 232, collection pipe; 232A, pipe body; 232A1, adapter inner cylinder; 232B, cover body; 232C, piston body; 232D, piston cylinder; 232E, filter cylinder; 232F, adapter rack; 232G, one-way valve; 232G1, discharge port; 232G2, valve body; 232G3, discharge channel; 232G4, force-bearing part; 232G5, connecting buckle;

[0136] 24. High rack;

[0137] 25, shake part; 251, shake lifting motor; 252, shake lifting screw rod; 253, shake lifting frame; 254, shake connecting frame; 255, shake drive part; 255A, shake drive motor; 255B, shake output gear; 255C, shake unit; 255C1, shake input gear; 255C2, shake spring; 255C3, shake rod; 255C4, shake limit pin; 255C5, shake claw; 255C6, transmission gear;

[0138] 3. Transfer position; 311. Transfer horizontal rack; 312. Transfer side slider; 313. Transfer bottom slider; 314. Transfer horizontal motor; 315. Transfer horizontal wheel; 316. Transfer horizontal belt;

[0139] 321, vertical pipetting frame; 322, vertical pipetting motor; 323, vertical pipetting screw rod; 324, vertical pipetting slide rail; 325, vertical pipetting sleeve; 326, pipette;

[0140] 4. Workbench; 411. Work rack; 412. Work slide rail; 413. Work motor; 414. Work wheel; 415. Work belt; 416. Barcode scanner; 421. Work storage rack; 422. Test tube; 423. Waste box;

[0141] 5. Purification position; 511. Purification horizontal frame; 512. Purification side slider; 513. Purification horizontal slide rail; 514. Purification bottom slider; 515. Purification horizontal motor; 516. Purification horizontal wheel; 517. Purification horizontal belt;

[0142] 52. Purification vertical unit;

[0143] 521A, purification vertical slide; 521B, purification first-level motor; 521C, purification horizontal slide; 521D, purification first-level screw rod; 521E, purification first-level slide rail; 521F, purification first-level slide;

[0144] 522A, purification first-stage frame; 522B, purification second-stage motor; 522C, purification second-stage screw rod; 522D, purification second-stage slide rail; 522E, purification second-stage slider;

[0145] 523A, purification secondary rack; 523B, purification magnetic rod; 523C, purification fixing plate; 523D, purification rebound member; 523D1, rod body; 523D2, cavity; 523D3, stopper; 523D4, return spring;

[0146] 523E, purification rebound plate; 523F, magnetic bar buckle; 523G, magnetic bar sleeve. DETAILED DESCRIPTION

[0147] The present application relates to a fully automatic methylation gene preparation workstation and a working method thereof, which are explained in detail below through specific implementation methods.

[0148] A fully automatic methylation gene preparation workstation, comprising:

[0149] The frame body 1 includes a bottom plate 11 and a stand 12 connected to the bottom plate 11;

[0150] The shaking and piercing station 2 is installed on the bottom plate 11 and connected to the stand 12, and is used to shake the sample in the collection tube 232 and discharge the sample into the storage container 222C;

[0151] The liquid transfer station 3 is connected to the stand 12 and is used to absorb the sample in the storage container 222C and move it to the test tube 422 placed in the workbench 4, and the sample includes feces;

[0152] A workbench 4, mounted on the bottom plate 11, for placing a test tube 422;

[0153] The purification position 5 is connected to the stand 12 and is used to purify the sample placed in the test tube 422.

[0154] The present invention is designed to shake the sample in the collection tube 232 by designing the shaking and piercing position 2, and discharge the sample into the storage container 222C, and then the sample in the storage container 222C is sucked by the liquid transfer position 3 and moved to the test tube 422 placed in the workbench 4, and finally the purification position 5 completes the purification of the sample in the test tube 422, thereby completing the preparation;

[0155] When the sample is shaken, the switching unit 221 drives the storage container 222C and the shaking seat 222D to switch with each other. When shaking, the shaking seat 222D contacts the collection tube 232, and the shaking portion 25 drives the shaking claw 255C5 to contact the cover 232B of the collection tube 232 and rotate to complete the shaking. When the shaken sample needs to be discharged, the storage container 222C is switched and contacts the one-way valve 232G of the collection tube 232. At the same time, the shaking rod 255C3 drives the piston body 232C to move, and the shaken sample is quickly discharged into the storage container 222C. , and then the sample in the storage vessel 222C is moved to the test tube 422 by the transfer position 3, and then the purification position 5 completes the purification work through the cooperation of the purification magnetic rod 523B and the magnetic rod sleeve 523G. After the workstation starts working, the whole process does not require manual intervention. Compared with the traditional semi-automatic workstation with manual intervention, the present application only needs to put the collection tube 232 into the tube rack 231 before the workstation works, and push the tube rack 231 into the low-level rack 21 to complete the manual work stage. The subsequent preparation work is completed by the workstation without manual intervention, realizing the work of sample in and gene out.

[0156] The stand 12 is provided with a driving unit 124, and the driving unit 124 includes:

[0157] A connecting plate 124C connected to the stand 12;

[0158] The driving member is designed in two groups and is installed on the connecting plate 124C. Each group includes driving wheels 124A arranged at both ends of the connecting plate 124C, a driving belt 124B sleeved on the driving wheels 124A, and a driving motor connected to the connecting plate 124C, and the output end of which is connected to any group of driving wheels 124A.

[0159] The inner side of the stand 12 is provided with a lateral slide rail 122 , and the top is provided with two sets of top slide rails 123 .

[0160] The transfer station 3 comprises:

[0161] The liquid transfer lateral unit includes a liquid transfer lateral linear module connected to the driving member and adapted to the top slide rail 123 and the lateral slide rail 122 and used to drive the liquid transfer vertical unit to move laterally;

[0162] The pipetting transverse linear module comprises a pipetting transverse frame 311, a pipetting slide rail mounted on the side of the pipetting transverse frame 311, two groups of pipetting transverse wheels 315 arranged on the pipetting transverse frame 311, a pipetting transverse belt 316 sleeved between the two groups of pipetting transverse wheels 315, and a pipetting transverse motor 314 connected to the pipetting transverse frame 311 and having an output end connected to any group of pipetting transverse wheels 315;

[0163] The transfer frame 311 has a transfer side slider 312 adapted to the lateral slide rail 122 at one end, and a transfer bottom slider 313 adapted to the top slide rail 123 at the bottom of the other end;

[0164] The pipetting horizontal frame 311 is connected to a driving belt 124B in a set of driving members;

[0165] The liquid transfer vertical unit includes a liquid transfer vertical lifting module connected to the liquid transfer horizontal linear module and used to drive the pipette 326 to perform lifting movement;

[0166] The pipette 326 is connected to the pipetting vertical unit.

[0167] The pipetting vertical lifting module includes a pipetting vertical frame 321 connected to the pipetting horizontal belt 316, a pipetting vertical motor 322 installed on the top of the pipetting vertical frame 321, a pipetting vertical screw rod 323 connected to the output end of the pipetting vertical motor 322, and a pipetting vertical sliding sleeve 325 sleeved on the pipetting vertical screw rod 323;

[0168] The pipette 326 is connected to the pipetting vertical sliding sleeve 325;

[0169] The pipetting vertical frame 321 is also provided with a pipetting vertical slide rail 324, and the pipetting vertical slide rail 324 is adapted to be provided with a pipetting vertical slide block connected to the pipetting vertical slide sleeve 325;

[0170] The back of the vertical pipetting frame 321 is provided with a pipetting slide block adapted to the pipetting slide rail.

[0171] By designing the transfer position 3, the sample in the storage container 222C is sucked and moved to the test tube 422 of the workbench 4 to complete the transfer process.

[0172] The purification position 5 comprises:

[0173] The purification horizontal unit includes a purification horizontal moving module for driving the purification vertical unit 52 to move horizontally;

[0174] The purification transverse moving module comprises a purification transverse frame 511, a purification transverse slide rail 513 installed on the side of the purification transverse frame 511, two groups of purification transverse wheels 516 installed on the top of the purification transverse frame 511, a purification transverse belt 517 sleeved on the two groups of purification transverse wheels 516, and a purification transverse motor 515 connected to the purification transverse frame 511 and having an output end connected to any one group of purification transverse wheels 516;

[0175] One end of the purification transverse frame 511 is provided with a purification side slider 512 adapted to the lateral slide rail 122, and the bottom of the other end is provided with a purification bottom slider 514 adapted to the top slide rail 123;

[0176] The purification transverse frame 511 is connected to the driving belt 124B in another set of driving members;

[0177] The purification vertical unit 52 includes a secondary lifting module connected to the purification horizontal moving module for driving the purification magnetic rod 523B in the purification unit to move up and down and separate the magnetic rod sleeve 523G and the magnetic rod buckle 523F;

[0178] The secondary lifting module includes a purification vertical slide 521A connected to the purification horizontal belt 517, a purification first-level motor 521B installed on the top of the purification vertical slide 521A, a purification first-level screw rod 521D connected to the output end of the purification first-level motor 521B (which can be coaxially connected, or gear-staggered meshing connection, or belt drive connection), a purification first-level frame 522A sleeved on the purification first-level screw rod 521D, a purification second-level motor 522B installed on the top of the purification first-level frame 522A, and a purification second-level screw rod 522C connected to the output end of the purification second-level motor 522B.

[0179] The purification vertical slide 521A is provided with a purification horizontal slide 521C adapted to the purification horizontal slide 513 on the back, and a purification primary slide 521E is installed on the front, and the purification primary slide 521E is adapted to be equipped with a purification primary slide 521F connected to the purification primary frame 522A;

[0180] The front of the purification primary rack 522A is equipped with a purification secondary slide rail 522D, and the purification secondary slide rail 522D is equipped with a purification secondary slider 522E;

[0181] The purification unit includes a purification fixed plate 523C fixedly connected to the secondary lifting module, and a purification secondary frame 523A sleeved on the secondary lifting module and moving up and down along the secondary lifting module;

[0182] The purification secondary rack 523A is connected to the purification secondary slider 522E;

[0183] The purification secondary frame 523A is sleeved on the purification secondary screw rod 522C;

[0184] The purification fixing plate 523C is arranged at the bottom of the purification primary frame 522A, and two groups of purification rebound members 523D are arranged on the purification fixing plate 523C. A plurality of magnetic rod buckles 523F with through cavities are embedded inside the purification fixing plate 523C;

[0185] A purification rebound plate 523E connected to two sets of purification rebound members 523D is provided below the purification fixing plate 523C;

[0186] The purification secondary frame 523A is equipped with multiple groups of purification magnetic rods 523B, and the purification magnetic rods 523B are adapted to the through cavity;

[0187] The purification resilient member 523D comprises a cavity 523D2 mounted on the purification fixing plate 523C, a rod 523D1 penetrating the cavity 523D2 and passing through the purification fixing plate 523C and connected to the purification resilient plate 523E, a stopper 523D3 sleeved on the rod 523D1 and located in the cavity 523D2, and a return spring 523D4 sleeved on the rod 523D1 and located between the stopper 523D3 and the bottom of the cavity 523D2;

[0188] When the purification secondary frame 523A is lowered to the low position, it contacts the rod body 523D1 in the purification rebound member 523D, and drives the purification rebound plate 523E to move, separating the magnetic rod sleeve 523G and the magnetic rod buckle 523F;

[0189] By designing the purification position 5, the purification work of the sample is completed, and after the purification work is completed, the purification secondary frame 523A is lowered to abut against the rod body 523D1 in the purification rebound member 523D, and the purification rebound plate 523E is driven to move, and the magnetic rod cover 523G is knocked off. The purified impurities are all attached to the magnetic rod cover 523G. At this time, the magnetic rod cover 523G is knocked off, so that the purification magnetic rod 523B can repeat the purification work of the sample.

[0190] The shaking and piercing position 2 comprises:

[0191] A switch piercing portion 22 is mounted on the bottom plate 11;

[0192] A low-level frame 21 is installed on the bottom plate 11 and is located above the switching piercing portion 22. A collection chute and a collection through slot are provided on the low-level frame 21. A first code scanner 416 is provided on the side of the low-level frame 21.

[0193] The collecting part 23 is arranged on the low frame 21 and is adapted to the collecting chute;

[0194] A high-position frame 24, located above the collecting portion 23 and connected to the vertical frame 12;

[0195] The shaking unit 25 is connected to the high-position frame 24 and is located above the collecting unit 23 .

[0196] The switching piercing portion 22 includes:

[0197] The switching unit 221 includes a switching movement driving member and a switching lifting driving member installed on the bottom plate 11 and used for driving the piercing unit 222 to move and lift;

[0198] The switching movement driving member includes a switching movement motor 221G and a switching movement wheel seat 221H mounted on the bottom plate 11, a switching movement wheel 1 arranged on the switching movement wheel seat 221H, a switching movement wheel 2 arranged at the output end of the switching movement motor 221G, and a switching movement belt 221I sleeved on the switching movement wheel 1 and the switching movement wheel 2;

[0199] The bottom plate 11 is also provided with a switching movable slide rail 221A, on which a switching movable slider is adapted, and on which a switching movable slide plate 221B connected to one side of the switching movable belt 221I is provided;

[0200] The switching lifting drive member includes a switching frame 221C connected to the switching moving slide 221B, a switching lifting slide rail 221D installed on the side of the switching frame 221C, a switching lifting slider adapted to the switching lifting slide rail 221D, a switching lifting plate 221E connected to the switching lifting slider, a switching lifting motor 221F connected to the switching frame 221C, and a switching lifting screw connected to the output end of the switching lifting motor 221F;

[0201] By designing the switching unit 221, the switching between the shaking seat 222D and the storage container 222C is completed. When the shaking is required, the shaking seat 222D is switched, and when the sample needs to be discharged, the storage container 222C is switched;

[0202] The piercing unit 222 includes a piercing plate 222A connected to the switching lifting plate 221E and sleeved on the switching lifting screw rod, a storage chute provided on the piercing plate 222A, a vessel rack 222B adapted to the storage chute, a plurality of storage vessels 222C placed in the vessel rack 222B, and a plurality of shaking seats 222D provided on the piercing plate 222A;

[0203] The shaking seat 222D includes a seat rod connected to the piercing plate 222A, and a seat body sleeved on the seat rod, and the seat body rotates along the seat rod.

[0204] The collecting part 23 includes a pipe rack 231 adapted to the collecting chute, and a plurality of collection pipes 232 arranged in the pipe rack 231;

[0205] The collection tube 232 includes:

[0206] The tube body 232A has a thread on the top and an adaptable inner cylinder 232A1 on the inside.

[0207] A cover body 232B is adapted to the thread and is disposed on the top of the tube body 232A. An extrusion through hole is provided on the top of the cover body 232B.

[0208] The piston cylinder 232D includes a cylinder body disposed in the tube body 232A, and a limiting body connected to the cylinder body and abutting against the top of the tube body 232A and located between the cover body 232B and the tube body 232A;

[0209] The piston body 232C is disposed in the cylinder body and is adapted to the cylinder body;

[0210] The filter cartridge 232E is disposed in the tube body 232A and is located below the tube body;

[0211] The adapter frame 232F is arranged between the adapter inner cylinder 232A1 and the filter cylinder 232E, and a bayonet is provided on the adapter frame 232F;

[0212] The one-way valve 232G is adapted to the adapting inner cylinder 232A1 and connected to the adapting frame 232F, and includes a connecting buckle 232G5 adapted to the bayonet, a valve body 232G2 connected to the connecting buckle 232G5 and located in the adapting inner cylinder 232A1, and a force-bearing portion 232G4 arranged at the bottom of the valve body 232G2 and located outside the adapting inner cylinder 232A1;

[0213] The valve body 232G2 has a discharge port 232G1 formed therein, and a discharge passage 232G3 communicating with the discharge port 232G1 and extending to the outside of the valve body 232G2;

[0214] The seat body is adapted to the tube body 232A, and the inlet end of the storage container 222C is adapted to the force-bearing portion 232G4;

[0215] The bottom of the pipe frame 231 is provided with a through groove adapted to the shaking seat 222D.

[0216] The collection tube 232 is designed with a one-way valve 232G, which cooperates with the storage container 222C to automatically discharge the sample. At the same time, a piston body 232C is designed, which cooperates with a lifting and lowering shaking rod 255C3 to quickly discharge the sample by pressing down and up.

[0217] The shaking unit 25 comprises:

[0218] The shaking and lifting part is used to drive the shaking driving part 255 and the shaking unit 255C to rise and fall, and includes a shaking and lifting motor 251 connected to the high-position frame 24, a shaking and lifting screw rod 252 connected to the output end of the shaking and lifting motor 251, and a shaking and lifting frame 253 sleeved on the shaking and lifting screw rod 252;

[0219] The shaking driving part 255 is used to drive the shaking unit 255C to complete the shaking of the sample, including a shaking driving motor 255A connected to the shaking lifting frame 253, a shaking driving gear sleeved on the output end of the shaking driving motor 255A, and a shaking output gear 255B meshing with the shaking driving gear;

[0220] The shaking unit 255C includes a plurality of shaking transmission members and shaking grabbing members which are staggered and meshed with each other;

[0221] Each set of shaking transmission parts includes a transmission rod and a transmission gear 255C6 sleeved on the transmission rod, and any set of transmission gears 255C6 is meshed with the shaking output gear 255B;

[0222] Each set of shaking grabbing members includes a shaking rod 255C3, a shaking input gear 255C1 sleeved on the shaking rod 255C3 and meshed with a transmission gear 255C6, and a shaking claw 255C5 disposed at the bottom of the shaking rod 255C3 and adapted to the cover 232B;

[0223] The transmission rod and the shaking rod 255C3 are both provided with a shaking bearing connected to the shaking lifting frame 253;

[0224] The shaking rod 255C3 is provided with a shaking slot, and the shaking claw 255C5 is provided with a shaking limit pin 255C4 adapted to the shaking slot;

[0225] A shaking spring 255C2 is sleeved between the shaking bearing of the shaking rod 255C3 and the shaking limit pin 255C4;

[0226] A shaking connecting frame 254 is connected between the high-position frame 24 and the low-position frame 21 , and a shaking slide rail is provided on the shaking connecting frame 254 , and a shaking sliding block connected to the shaking lifting frame 253 is provided on the shaking slide rail.

[0227] Through the design of the shaking slide, the remote control rod can be raised and lowered, thereby driving the piston body 232C to move, and the shaking claw 255C5 is reset through the shaking spring 255C2 to avoid delaying the next shaking work of the shaking claw 255C5.

[0228] The workbench 4 comprises:

[0229] The storage part is arranged on the base, including a pull-out track installed on the bottom plate 11, a working storage rack 421 arranged on the pull-out track and adapted to the pull-out track, and a test tube 422, a waste box 423, and a magnetic rod sleeve 523G are arranged on the working storage rack 421. A temperature control module is arranged inside the storage rack to control the temperature of the test tube 422. The temperature control module belongs to the prior art and can be formed by a heat exchanger of a heating module and a refrigeration module;

[0230] The code scanning unit is arranged on the side of the working storage rack 421, including a working rack 411 installed on the bottom plate 11, and a code scanning component arranged on the working rack 411 for driving the code scanner 416 to move along the working rack 411.

[0231] The code scanning component includes a working slide rail 412, two sets of working wheels 414, a working belt 415 sleeved on the two sets of working wheels 414, and a working motor 413 connected to any set of working wheels 414, which are arranged on the working frame 411;

[0232] The working slide rail 412 is provided with a working slide block, and the working slide block is installed with a second code scanner 416 .

[0233] A working method of a fully automatic methylation gene preparation workstation, comprising:

[0234] Step 1: Place the collection tube 232 containing the test sample into the tube rack 231, and slide the tube rack 231 into the low-level rack 21 through the collection chute;

[0235] Step 2: Switch the moving drive member and the lifting drive member to drive the shaking seat 222D to move to the bottom of the collection tube 232 and press against the collection tube 232;

[0236] Step 3, the shaking lifting unit drives the shaking driving unit 255 to complete the grabbing of the collection tube 232, and the shaking driving unit 255 completes the shaking work;

[0237] The shaking and lifting part drives the shaking and lifting screw 252 to move through the shaking and lifting motor 251, drives the shaking and lifting frame 253 to move up and down along the shaking slide rail, and drives the shaking claw 255C5 of the shaking driving part 255 to contact the cover 232B of the collection tube 232;

[0238] The shaking driving unit 255 drives the shaking driving gear 255A to rotate, drives the shaking output gear 255B to rotate, drives the transmission gear 255C6 to rotate, drives the shaking input gear 255C1 to rotate, drives the shaking rod 255C3 to rotate, drives the shaking claw 255C5 to rotate, drives the collection tube 232 to rotate, and completes the sample shaking work;

[0239] Step 4: After the sample is shaken, the moving drive member and the lifting drive member are switched to drive the shaking seat 222D to separate from the bottom of the collection tube 232, and the storage container 222C is driven to move to the bottom of the collection tube 232;

[0240] Step 5, after the storage container 222C is switched, the switching moving drive member moves, the storage container 222C is pressed against the one-way valve 232G, and continues to move upward. At this time, the storage container 222C presses against the force-bearing part 232G4 to drive the valve body 232G2 to rise. At this time, the discharge port 232G1 contacts the sample stored in the tube body 232A. The sample is filtered through the filter cartridge 232E, and is discharged into the storage container 222C through the discharge port 232G1 and the discharge channel 232G3. When the sample is discharged, the shaking and lifting motor 251 drives the shaking and lifting screw rod 252 to move, and drives the shaking and lifting frame 253 to continue to move downward along the shaking slide rail, so that the shaking rod 255C3 drives the piston body 232C to descend in the piston cylinder 232D through the extrusion through hole, thereby accelerating the discharge speed of the sample;

[0241] Step 6: After the sample is discharged, the liquid transfer position 3 completes the liquid transfer work, and the storage container 222C is driven to move to a predetermined position by switching the moving drive member and the lifting drive member, and the liquid transfer position 3 moves the sample to the test tube 422 on the workbench 4;

[0242] When the switching moving driving member moves, the switching moving motor 221G drives the switching moving wheel 2 to move, and drives the switching moving belt 221I to move, and then drives the switching moving slide plate 221B to slide along the switching moving slide rail 221A, so as to complete the lifting movement of the switching lifting driving member;

[0243] When the switch lifting drive member moves, the switch lifting motor 221F drives the piercing plate 222A to rise along the switch lifting rail 221D, thereby completing the position adjustment and height adjustment of the shaking seat 222D and the storage container 222C;

[0244] During transfer, the pipette 326 is driven to move back and forth between the storage container 222C and the test tube 422 by the transfer horizontal unit and the transfer vertical unit to transfer the sample into the test tube 422;

[0245] The transverse unit of the liquid transfer is driven by the transverse motor 314 to move the transverse wheel 315, which drives the transverse belt 316 to move, and drives the vertical frame 321 to move along the liquid transfer rail, thereby driving the transverse movement of the vertical unit of the liquid transfer.

[0246] The vertical transfer unit is driven by the vertical transfer motor 322 to rotate the vertical transfer screw 323, which drives the vertical transfer sleeve 325 to move along the vertical transfer rail 324, thereby driving the pipette 326 to work;

[0247] Step 7, the purification work of the sample in the test tube 422 is completed through the purification position 5, and the purification horizontal unit and the purification vertical unit 52 cooperate to drive the magnetic bar buckle 523F in the purification unit to engage with the magnetic bar sleeve 523G placed on the workbench 4, and drive the purification magnetic bar 523B to extend into the magnetic bar sleeve 523G, and move the magnetic bar sleeve 523G into the test tube 422 to complete the purification of the sample in the test tube 422;

[0248] After the sample is purified, the purification secondary rack 523A is driven to continue to descend through the purification vertical unit 52 until it contacts the rod body 523D1 in the purification rebound member 523D, and drives the purification rebound plate 523E to move downward, separating the magnetic rod sleeve 523G and the magnetic rod buckle 523F, and completing the preparation. During separation, the magnetic rod sleeve 523G is moved to the top of the waste box 423;

[0249] The purification horizontal unit is driven by the purification horizontal motor 515 to move the purification horizontal wheel 516, drive the purification horizontal belt 517 to move, drive the purification vertical slide 521A to move along the purification horizontal slide rail 513, and complete the movement of the purification vertical unit 52;

[0250] The purification vertical unit 52 is driven by the purification first-level motor 521B to drive the purification first-level screw rod 521D to rotate, driving the purification first-level frame 522A to move up and down, and then the purification second-level motor 522B drives the purification second-level screw rod 522C to rotate, driving the purification second-level frame 523A to move up and down.

[0251] Working principle description: put the collection tube 232 containing the test sample into the tube rack 231, and slide the tube rack 231 into the low-level rack 21 through the collection slide; drive the shaking seat 222D to move below the collection tube 232 by switching the moving drive member and switching the lifting drive member, and press against the collection tube 232; drive the shaking drive member 255 to grab the collection tube 232 through the shaking lifting member, and the shaking drive member 255 completes the shaking work;

[0252] The shaking and lifting part drives the shaking and lifting screw 252 to move through the shaking and lifting motor 251, drives the shaking and lifting frame 253 to move up and down along the shaking slide rail, and drives the shaking claw 255C5 of the shaking driving part 255 to contact the cover 232B of the collection tube 232;

[0253] The shaking driving unit 255 drives the shaking driving gear 255A to rotate, drives the shaking output gear 255B to rotate, drives the transmission gear 255C6 to rotate, drives the shaking input gear 255C1 to rotate, drives the shaking rod 255C3 to rotate, drives the shaking claw 255C5 to rotate, drives the collection tube 232 to rotate, and completes the sample shaking work;

[0254] After the sample is shaken, the shaking seat 222D is driven to separate from the bottom of the collection tube 232 by switching the moving drive member and the switching lifting drive member, and the storage container 222C is driven to move to the bottom of the collection tube 232; after the storage container 222C is switched, the moving drive member is switched to move, and the storage container 222C is pressed against the one-way valve 232G and continues to move upward. At this time, the storage container 222C presses against the force-bearing part 232G4 to drive the valve body 232G2 to rise. At this time, the discharge port 232G1 contacts the sample stored in the tube body 232A, and the sample is filtered through the filter cartridge 232E and passes through the discharge port 23 2G1 and discharge channel 232G3, discharged into the storage container 222C, when the sample is discharged, the shaking and lifting motor 251 drives the shaking and lifting screw rod 252 to move, and drives the shaking and lifting frame 253 to continue to move downward along the shaking slide rail, so that the shaking rod 255C3 drives the piston body 232C to descend in the piston cylinder 232D through the extrusion through hole, thereby accelerating the discharge speed of the sample; after the sample is discharged, the liquid transfer position 3 completes the liquid transfer work, and the storage container 222C is driven to move to a predetermined position by switching the moving drive member and switching the lifting drive member, and the sample is moved to the test tube 422 of the workbench 4 by the liquid transfer position 3;

[0255] When the switching moving driving member moves, the switching moving motor 221G drives the switching moving wheel 2 to move, and drives the switching moving belt 221I to move, and then drives the switching moving slide plate 221B to slide along the switching moving slide rail 221A, so as to complete the lifting movement of the switching lifting driving member;

[0256] When the switch lifting drive member moves, the switch lifting motor 221F drives the piercing plate 222A to rise along the switch lifting rail 221D, thereby completing the position adjustment and height adjustment of the shaking seat 222D and the storage container 222C;

[0257] During transfer, the pipette 326 is driven to move back and forth between the storage container 222C and the test tube 422 by the transfer horizontal unit and the transfer vertical unit to transfer the sample into the test tube 422;

[0258] The transverse unit of the liquid transfer is driven by the transverse motor 314 to move the transverse wheel 315, which drives the transverse belt 316 to move, and drives the vertical frame 321 to move along the liquid transfer rail, thereby driving the transverse movement of the vertical unit of the liquid transfer.

[0259] The vertical transfer unit is driven by the vertical transfer motor 322 to rotate the vertical transfer screw 323, which drives the vertical transfer sleeve 325 to move along the vertical transfer rail 324, thereby driving the pipette 326 to work;

[0260] The purification work of the sample in the test tube 422 is completed through the purification position 5. The purification horizontal unit and the purification vertical unit 52 work together to drive the magnetic bar buckle 523F in the purification unit to engage with the magnetic bar sleeve 523G placed on the workbench 4, and drive the purification magnetic bar 523B to extend into the magnetic bar sleeve 523G, and move the magnetic bar sleeve 523G into the test tube 422 to complete the purification of the sample in the test tube 422;

[0261] After the sample is purified, the purification secondary rack 523A is driven to continue to descend through the purification vertical unit 52 until it contacts the rod body 523D1 in the purification rebound member 523D, and drives the purification rebound plate 523E to move downward, separating the magnetic rod sleeve 523G and the magnetic rod buckle 523F, and completing the preparation. During separation, the magnetic rod sleeve 523G is moved to the top of the waste box 423;

[0262] The purification horizontal unit is driven by the purification horizontal motor 515 to move the purification horizontal wheel 516, drive the purification horizontal belt 517 to move, drive the purification vertical slide 521A to move along the purification horizontal slide rail 513, and complete the movement of the purification vertical unit 52;

[0263] The purification vertical unit 52 is driven by the purification first-level motor 521B to drive the purification first-level screw rod 521D to rotate, driving the purification first-level frame 522A to move up and down, and then the purification second-level motor 522B drives the purification second-level screw rod 522C to rotate, driving the purification second-level frame 523A to move up and down.

[0264] The preferred specific embodiments of the present invention are described in detail above in conjunction with the accompanying drawings. However, the present invention is not limited to the specific details in the above specific embodiments. Within the technical concept of the present invention, various equivalent transformations can be made to the technical scheme of the present invention, and these equivalent transformations all belong to the protection scope of the present invention.

Claims

1. A fully automatic methylation gene preparation workstation, characterized in that: include: The frame (1) comprises a bottom plate (11) and a stand (12) connected to the bottom plate (11); A shaking and piercing station (2), installed on the bottom plate (11) and connected to the stand (12), is used to shake the sample in the collection tube (232) and discharge the sample into the storage container (222C); A liquid transfer station (3) connected to the stand (12) for aspirating the sample in the storage container (222C) and moving it to a test tube (422) placed in the workbench (4); A workbench (4), mounted on the bottom plate (11), for placing a test tube (422); The purification station (5) is connected to the stand (12) and is used to purify the sample placed in the test tube (422).

2. A fully automatic methylation gene preparation workstation according to claim 1, characterized in that: Said A driving unit (124) is provided on the stand (12), and the driving unit (124) comprises: A connecting plate (124C) connected to the stand (12); The driving member is designed in two groups and is installed on the connecting plate (124C), each group comprising driving wheels (124A) arranged at both ends of the connecting plate (124C), a driving belt (124B) sleeved on the driving wheels (124A), and a driving motor connected to the connecting plate (124C) and having an output end connected to any one group of driving wheels (124A); The inner side of the stand (12) is provided with a lateral slide rail (122), and the top is provided with two groups of top slide rails (123).

3. The fully automatic methylation gene preparation workstation according to claim 1, characterized in that: The transfer station (3) comprises: The liquid transfer lateral unit comprises a liquid transfer lateral linear module connected to the driving member and adapted to the top slide rail (123) and the lateral slide rail (122) and used to drive the liquid transfer vertical unit to move laterally; A liquid transfer vertical unit, comprising a liquid transfer vertical lifting module connected to the liquid transfer horizontal linear module and used to drive the pipette (326) to perform lifting movement; A pipette (326) is connected to the pipetting vertical unit.

4. The fully automatic methylation gene preparation workstation according to claim 1, characterized in that: The purification position (5) comprises: The purification horizontal unit comprises a purification horizontal moving module for driving the purification vertical unit (52) to move horizontally; A purification vertical unit (52) comprises a secondary lifting module connected to the purification horizontal moving module and used to drive the purification magnetic rod (523B) in the purification unit to move up and down and separate the magnetic rod sleeve (523G) and the magnetic rod buckle (523F); The purification unit comprises a purification fixed plate (523C) fixedly connected to the secondary lifting module, and a purification secondary frame (523A) sleeved on the secondary lifting module and lifting and moving along the secondary lifting module; The purification fixing plate (523C) is provided with two groups of purification resilient members (523D), and a plurality of magnetic bar buckles (523F) with through cavities are embedded inside the purification fixing plate (523C); A purification rebound plate (523E) connected to two groups of purification rebound members (523D) is provided below the purification fixing plate (523C); The purification secondary frame (523A) is equipped with a plurality of purification magnetic rods (523B), and the purification magnetic rods (523B) are adapted to the through cavity; When the purification secondary frame (523A) is lowered to a low position, it contacts the purification rebound member (523D) and drives the purification rebound plate (523E) to move, thereby separating the magnetic rod sleeve (523G) and the magnetic rod buckle (523F).

5. The fully automatic methylation gene preparation workstation according to claim 1, characterized in that: The shaking piercing site (2) comprises: A switch piercing portion (22) is mounted on the bottom plate (11); A low-position frame (21) is installed on the bottom plate (11) and is located above the switching piercing portion (22). A collection chute and a collection through groove are provided on the low-position frame (21); A collecting part (23) is arranged on the low-position frame (21) and is adapted to the collecting chute; A high-position frame (24), located above the collecting portion (23) and connected to the vertical frame (12); The shaking part (25) is connected to the high-position frame (24) and is located above the collecting part (23).

6. A fully automatic methylation gene preparation workstation according to claim 5, characterized in that: The switching piercing portion (22) comprises: The switching unit (221) comprises a switching movement driving member and a switching lifting driving member installed on the bottom plate (11) and used for driving the piercing unit (222) to move and lift; The piercing unit (222) includes a lifting drive connected to a piercing plate (222A), a storage chute provided on the piercing plate (222A), a vessel rack (222B) adapted to the storage chute, a plurality of storage vessels (222C) placed in the vessel rack (222B), and a plurality of shaking seats (222D) arranged on the piercing plate (222A).

7. The fully automatic methylation gene preparation workstation according to claim 5, characterized in that: The collecting part (23) comprises a pipe rack (231) adapted to the collecting chute, and a plurality of groups of collecting pipes (232) arranged in the pipe rack (231); The collection tube (232) comprises: A tube body (232A), wherein the outer side of the top of the tube body (232A) is provided with a thread, and the inside of the tube body (232A) is provided with an adaptable inner cylinder (232A1); A cover body (232B) adapted to the thread and arranged on the top of the tube body (232A), and an extrusion through hole is arranged on the top of the cover body (232B); The piston cylinder (232D) comprises a cylinder body arranged in the tube body (232A), and a limiting body connected to the cylinder body, abutting against the top of the tube body (232A) and located between the cover body (232B) and the tube body (232A); A piston body (232C) is disposed in the cylinder body and is adapted to the cylinder body; A filter cartridge (232E) is disposed in the tube body (232A) and is located below the cartridge body; An adapter frame (232F) is arranged between the adapter inner cylinder (232A1) and the filter cylinder (232E), and a bayonet is provided on the adapter frame (232F); A one-way valve (232G) adapted to the adapting inner cylinder (232A1) and connected to the adapting frame (232F), comprising a connecting buckle (232G5) adapted to the bayonet, a valve body (232G2) connected to the connecting buckle (232G5) and located in the adapting inner cylinder (232A1), and a force-bearing portion (232G4) arranged at the bottom of the valve body (232G2) and located outside the adapting inner cylinder (232A1); The valve body (232G2) is provided with a discharge port (232G1) and a discharge passage (232G3) which is connected to the discharge port (232G1) and extends to the outside of the valve body (232G2).

8. The fully automatic methylation gene preparation workstation according to claim 5, characterized in that: The shaking unit (25) comprises: A shaking lifting unit, used for driving the shaking driving unit (255) and the shaking unit (255C) to move up and down; A shaking driving unit (255) is used to drive the shaking unit (255C) to complete the shaking of the sample; A shaking unit (255C) comprises a plurality of shaking transmission parts and shaking grabbing parts which are staggered and meshed with each other; Each set of shaking transmission parts includes a transmission rod and a transmission gear (255C6) sleeved on the transmission rod; Each set of shaking grabbing members comprises a shaking rod (255C3), a shaking input gear (255C1) sleeved on the shaking rod (255C3) and meshed with a transmission gear (255C6), and a shaking claw (255C5) arranged at the bottom of the shaking rod (255C3) and adapted to the cover body (232B); The shaking rod (255C3) is provided with a shaking slot, and the shaking claw (255C5) is provided with a shaking limit pin (255C4) adapted to the shaking slot; A shaking spring (255C2) is sleeved between the shaking bearing of the shaking rod (255C3) and the shaking limit pin (255C4).

9. The fully automatic methylation gene preparation workstation according to claim 1, characterized in that: The workbench (4) comprises: A storage portion, arranged on the base, comprising a pull-out track mounted on the bottom plate (11), a working storage rack (421) arranged on the pull-out track and adapted to the pull-out track, wherein the working storage rack (421) is provided with a test tube (422), a waste box (423), and a magnetic rod sleeve (523G); The code scanning unit is arranged on the side of the working storage rack (421), and comprises a working rack (411) installed on the bottom plate (11), and a code scanning member arranged on the working rack (411) for driving a code scanner (416) to move along the working rack (411).

10. A working method of a fully automatic methylation gene preparation workstation, implemented based on a fully automatic methylation gene preparation workstation according to any one of claims 1 to 9, characterized in that: include: Step 1: Place the collection tube (232) containing the test sample into the tube rack (231), and slide the tube rack (231) into the low-level rack (21) through the collection chute; Step 2, by switching the moving drive member and the lifting drive member, the shaking seat (222D) is driven to move to the bottom of the collection tube (232) and abut against the collection tube (232); Step 3, the shaking lifting unit drives the shaking driving unit (255) to complete the grabbing of the collection tube (232), and the shaking driving unit (255) completes the shaking work; Step 4: After the sample is shaken, the shaking seat (222D) is driven to separate from the bottom of the collection tube (232) by switching the moving drive member and the lifting drive member, and the storage container (222C) is driven to move to the bottom of the collection tube (232); Step 5, after the storage container (222C) is switched, the switching moving drive member moves, the storage container (222C) is pressed against the one-way valve (232G), and continues to move upward. At this time, the storage container (222C) presses against the force-bearing part (232G4) to drive the valve body (232G2) to rise. At this time, the discharge port (232G1) contacts the sample stored inside the tube body (232A), and the sample is filtered through the filter cartridge (232E), and is discharged into the storage container (222C) through the discharge port (232G1) and the discharge channel (232G3). When the sample is discharged, the shaking and lifting motor (251) drives the shaking and lifting screw rod (252) to move, and drives the shaking and lifting frame (253) to continue to move downward along the shaking slide rail, so that the shaking rod (255C3) drives the piston body (232C) to descend in the piston cylinder (232D) through the extrusion through hole, thereby accelerating the discharge speed of the sample; Step 6: After the sample is discharged, the liquid transfer position (3) completes the liquid transfer work, and the storage container (222C) is driven to move to a predetermined position by switching the moving drive member and switching the lifting drive member, and the liquid transfer position (3) moves the sample to the test tube (422) on the workbench (4); During liquid transfer, the liquid transfer transverse unit and the liquid transfer vertical unit drive the liquid transfer device (326) to and fro between the storage container (222C) and the test tube (422), so as to transfer the sample into the test tube (422); Step 7, complete the purification of the sample in the test tube (422) through the purification position (5), and drive the magnetic bar buckle (523F) in the purification unit to engage with the magnetic bar sleeve (523G) placed on the workbench (4) through the cooperation of the purification horizontal unit and the purification vertical unit (52), and drive the purification magnetic bar (523B) to extend into the magnetic bar sleeve (523G), and move the magnetic bar sleeve (523G) into the test tube (422), so as to complete the purification of the sample in the test tube (422); After the sample is purified, the purification secondary frame (523A) is driven to continue to descend through the purification vertical unit (52) until it contacts the rod body (523D1) in the purification rebound member (523D), and drives the purification rebound plate (523E) to move downward, separating the magnetic rod sleeve (523G) and the magnetic rod buckle (523F), thereby completing the preparation.

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