PCR reaction device

Abstract

The invention relates to the field of biological detection, and particularly discloses a PCR (Polymerase Chain Reaction) reaction device which comprises a main body, the sample table is arranged in the main body, and the sample table can be used for placing sample liquid; the number of the eight-connected pipes is multiple, the multiple eight-connected pipes are arranged in the sample table at equal intervals, and the eight-connected pipes are used for containing detection samples; the fixed table is arranged above the sample table; after a movable seat presses a connecting plate, an inclined assembly begins to operate, and a lifting plate can synchronously descend, so that an opening in the bottom end of the lifting plate is in direct contact with an adjusting block, the adjusting block rotates in the direction of an arc-shaped opening in the bottom end of the lifting plate, and then the whole eight-connection pipe begins to slightly incline in one direction to wait for a liquid injection pipe to inject liquid into the eight-connection pipe; the inclined eight-connection tube can effectively enable sample liquid to flow downwards along the inside of the eight-connection tube, and prevent the sample liquid from splashing out of the test tube, so that an experimenter and an experiment environment are protected.

Description

Technical Field

[0001] The invention relates to the field of biological detection, in particular to a PCR reaction device. Background Art

[0002] PCR is a widely used technique in molecular biology for rapid amplification of specific DNA fragments in vitro. The PCR reaction device is a key device for performing PCR experiments. It integrates multiple functions and features to ensure that the PCR reaction can be carried out efficiently and accurately. Real-time fluorescence quantitative PCR is a quantitative analysis technology for detecting DNA. The intensity of the fluorescence signal during the PCR reaction is measured to determine the number of PCR products. For this purpose, we propose a PCR reaction device.

[0003] However, the existing PCR instrument is usually loaded manually using eight-tubes. However, this loading method requires the experimenter to be very careful and accurate in placing the eight-tube into the sample tray, otherwise the sample liquid in the eight-tube may adhere to the wall droplets, and water will come out from the bottom of the eight-tube, which may cause cross contamination and lead to inaccurate test results. For this reason, we propose an orthopedic anesthesia monitoring and control device. Summary of the invention

[0004] The object of the present invention is to provide a PCR reaction device to solve the problem proposed in the above background technology that the existing PCR instrument is usually loaded manually using eight-tube strips. However, this loading method requires the experimenter to place the eight-tube strips into the sample tray very carefully and accurately, otherwise the sample liquid in the eight-tube strips may adhere to the wall droplets, and water will come out from the bottom of the eight-tube strips, which may cause cross contamination and lead to inaccurate test results.

[0005] To achieve the above-mentioned object, the present invention provides the following technical solution: A PCR reaction device, comprising: a main body;

[0006] Also includes:

[0007] A sample stage, which is arranged inside the main body and can be used to place sample liquid;

[0008] An eight-tube strip, wherein a plurality of the eight-tube strips are provided, and the plurality of the eight-tube strips are equidistantly arranged inside the sample stage, and the eight-tube strips are used to place the test samples;

[0009] A fixing platform, wherein the fixing platform is arranged above the sample platform;

[0010] An injection tube, which is arranged at the front end of the fixed platform and is used for quantitatively injecting the sample liquid;

[0011] A tilting assembly, the tilting assembly is arranged inside the sample stage, the tilting assembly is used to tilt the eight-tube strip one by one according to the position of the sample, the tilting assembly includes a connecting rod, both sides of the connecting rod are penetrated by lifting rods, the lifting rods are slidably connected to the fixed platform, the top ends of the two lifting rods are fixedly connected to a connecting plate, the two ends of the connecting plate are inclined, the outer walls of the two lifting rods are provided with a reset spring, the two ends of the connecting rod are fixedly connected to a lifting plate, the lifting plate is slidably connected to the fixed platform, the lifting plate is arranged in a bent shape, and the bottom end of the lifting plate is arranged to open downward;

[0012] Transmission assembly, wherein two transmission assemblies are provided, the two transmission assemblies are symmetrically arranged about the central axis of the fixed platform, the transmission assembly is arranged inside the fixed platform, and the transmission assembly is used for equidistantly pushing the eight-linked tube of the rod.

[0013] Wherein, both ends of the eight-tube are fixedly connected with a rotating shaft, the outer side of the rotating shaft is engaged with a clamping block, the clamping block is rotatably connected to the sample stage, and the rear end side of the clamping block is fixedly connected with an adjusting block.

[0014] The bottom end of the lifting plate is in an arc shape, the bottom end opening of the lifting plate faces downward, and the position of the lifting plate corresponds to the position of the adjusting block.

[0015] Among them, a transmission screw is rotatably connected to the upper side of the fixed platform, a rotating rod is arranged above the transmission screw, both ends of the transmission screw and the rotating rod are fixedly connected with an adjustment plate, a plurality of transmission grooves are opened on the outer wall of the adjustment plate, and the plurality of transmission grooves are distributed in a circular array about the central axis of the adjustment plate, a transmission plate is transmission-connected between the two adjustment plates, a transmission block is fixedly connected to the bottom end of the transmission plate, the transmission block is arranged inside the transmission groove, the two adjustment plates are symmetrically distributed about the central axis of the transmission plate, and a servo motor is installed at the rear end of the adjustment plate.

[0016] The outer wall of the transmission screw is threadedly connected to a moving seat, a sliding rod is slidably connected to the lower side of the moving seat, and the sliding rod is fixedly connected to the fixed platform.

[0017] Among them, the outer wall of the rotating rod is provided with a limiting groove, and the rotating rod is slidably connected to a worm wheel through the limiting groove, and a sliding member is provided on the side of the worm wheel, and the sliding member is rotatably connected to the movable seat, and the side of the worm wheel is meshingly connected with a worm, and the bottom end of the worm is fixedly connected to a movable rod, and the outer wall of the movable rod is provided with two movable grooves, and the two movable grooves are cross-arranged, and a sliding block is slidably connected inside the movable groove, and the front end of the sliding block is movably connected to a lifting seat, and the front end of the lifting seat is fixedly connected to the injection tube.

[0018] Among them, the bottom end of the injection tube is fixedly connected to the first fixed cylinder, the outer wall of the first fixed cylinder is slidably connected to the second fixed cylinder, the second fixed cylinder is fixedly connected to the movable seat, the bottom end of the first fixed cylinder is slidably connected to the injection tube, the inlet of the first fixed cylinder is provided with a first one-way valve, the outlet of the injection tube is provided with a second one-way valve, and the bottom end of the injection tube is inclined.

[0019] Among them, the transmission assembly includes a pressing rod, the outer wall of the pressing rod is fixedly connected to a sliding block, the outer wall of the sliding block is slidably connected to a sliding shell, the inner wall of the pressing rod is provided with a positioning block, the inside of the pressing rod is slidably connected to an adjusting rod, the outer wall of the adjusting rod begins to have an inclined groove, the end of the inclined groove is provided with a vertical groove, the bottom end of the adjusting rod is fixedly connected to an adjusting gear, the bottom end of the adjusting gear is transmission-connected to a transmission tooth plate, the transmission tooth plate is fixedly connected to the sample platform, and a pressure sensor is installed at the bottom end of the sliding block.

[0020] The depth of the vertical groove is deeper than that of the inclined groove, the positioning block is embedded in the inclined groove, the bottom end of the positioning block is fixedly connected with a connecting spring, and the positioning block is movably connected to the positioning block through the connecting spring.

[0021] The sliding shell is slidably connected to the main body, a fixing rod is fixedly connected to the top of the sliding shell, a fixing nut is threadedly connected to the outer wall of the fixing rod, and the fixing nut is rotatably connected to the main body.

[0022] The present invention has at least the following beneficial effects:

[0023] 1. After the moving seat is pressed onto the connecting plate, the tilting assembly starts to operate, so that the lifting plate can be synchronously lowered, so that the bottom opening of the lifting plate will directly contact the adjustment block, and the adjustment block will rotate along the arc-shaped opening at the bottom of the lifting plate, and then the entire eight-tube starts to tilt slightly in one direction, waiting for the injection tube to inject liquid into the eight-tube. The tilted eight-tube can effectively make the sample liquid flow downward along the inside of the eight-tube, and prevent the sample liquid from splashing out in the test tube, protecting the experimenter and the experimental environment;

[0024] 2. When the moving seat moves to both sides of the fixed platform driven by the transmission screw, the moving seat will directly touch the pressing rod, and at the same time drive the pressing rod to move backward inside the fixed shell through the sliding block, and then the positioning block in the pressing rod slides in the inclined groove. While sliding, it drives the adjusting rod and the adjusting gear to rotate half a circle, so that the adjusting gear drives the transmission gear plate to move. During the movement, the eight-tube package that has been injected is sent into the main body, and the next row of eight-tube package is sent to the bottom of the injection tube. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1It is a front view structural schematic diagram of the present invention;

[0026] Figure 2 It is a side view structural schematic diagram of the present invention;

[0027] Figure 3 This is a schematic diagram of the eight-tube structure of the present invention;

[0028] Figure 4 It is a schematic diagram of the structure of the sample stage of the present invention;

[0029] Figure 5 This is a schematic diagram of the structure of the tilting assembly of the present invention;

[0030] Figure 6 This is a schematic diagram of the connection structure between the transmission screw and the moving seat of the present invention;

[0031] Figure 7 This is a schematic diagram of the connection structure between the adjustment plate and the transmission plate of the present invention;

[0032] Figure 8 This is a schematic diagram of the connection structure between the movable rod and the injection tube of the present invention;

[0033] Fig. 9 This is a schematic diagram of the cutaway structure of the first fixed tube of the present invention;

[0034] Fig.10 This is a schematic diagram of the cutaway structure of the second fixed tube of the present invention;

[0035] Fig.11 This is a schematic diagram of the cutaway structure of the sliding shell of the present invention;

[0036] Fig.12 This is a schematic diagram of the cross-section structure of the pressing rod of the present invention;

[0037] Fig.13 For the present invention Fig.12 Enlarged structural diagram at A in the middle.

[0038] In the figure: 001, main body; 002, sample table; 003, eight-tube; 004, fixed table; 005, injection tube; 006, tilting assembly; 007, transmission assembly; 310, rotating shaft; 320, clamping block; 330, adjusting block; 410, transmission screw; 420, rotating rod; 430, adjusting plate; 440, transmission slot; 450, transmission plate; 460, transmission block; 470, servo motor; 411, moving seat; 412, sliding rod; 421, limiting slot; 422, worm gear; 423, sliding member; 424, worm; 425, movable rod; 426, moving slot; 427, slider ;428, lifting seat; 510, first fixed cylinder; 520, second fixed cylinder; 530, first one-way valve; 540, injection tube; 550, second one-way valve; 601, connecting rod; 602, lifting rod; 603, connecting plate; 604, reset spring; 605, lifting plate; 701, pressing rod; 702, sliding block; 703, sliding shell; 704, positioning block; 705, adjusting rod; 706, inclined groove; 707, vertical groove; 708, adjusting gear; 709, transmission gear plate; 710, pressure sensor; 720, connecting spring; 730, fixing rod; 740, fixing nut. DETAILED DESCRIPTION

[0039] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0040] Embodiment 1

[0041] See also Figures 1 to 10 ,The present invention provides a technical solution: a PCR reaction device, comprising: a main body 001;

[0042] Also includes:

[0043] A sample stage 002, which is disposed inside the main body 001 and can be used to place a sample liquid;

[0044] An eight-tube joint 003, wherein a plurality of the eight-tube joints 003 are provided, and the plurality of the eight-tube joints 003 are equidistantly arranged inside the sample stage 002, and the eight-tube joints 003 are used to place the test samples;

[0045] A fixed platform 004, wherein the fixed platform 004 is disposed above the sample platform 002;

[0046] An injection tube 005, which is disposed at the front end of the fixed platform 004 and is used for quantitatively injecting sample liquid;

[0047] A tilting assembly 006, wherein the tilting assembly 006 is arranged inside the sample stage 002, and the tilting assembly 006 is used to tilt the eight-tube joint 003 one by one according to the position of the sample, and the tilting assembly 006 includes a connecting rod 601, and both sides of the connecting rod 601 are penetrated by lifting rods 602, and the lifting rods 602 are slidably connected to the fixed platform 004, and the top ends of the two lifting rods 602 are fixedly connected with connecting plates 603, and the two ends of the connecting plates 603 are inclined, and the outer walls of the two lifting rods 602 are provided with reset springs 604, and both ends of the connecting rod 601 are fixedly connected with lifting plates 605, and the lifting plates 605 are slidably connected to the fixed platform 004, and the lifting plates 605 are arranged in a bent shape, and the bottom end of the lifting plates 605 is arranged to open downward;

[0048] Transmission assembly 007, two transmission assemblies 007 are provided, and the two transmission assemblies 007 are symmetrically arranged about the central axis of the fixed platform 004. The transmission assembly 007 is arranged inside the fixed platform 004, and the transmission assembly 007 is used for equidistantly pushing the rod eight-link tube 003.

[0049] Among them, the staff will pull out the sample table 002 from the main body 001 according to the test needs, and the pulled out eight-tube 003 will be used as the test tube to be tested. Then, the test sample will be injected into the injection tube 005. After starting the servo motor 470, the moving seat 411 drives the injection tube 005 to enter the position of the eight-tube 003. At the same time, the moving seat 411 will press down the connecting plate 603 and the two lifting rods 602 when moving, so that the tilting component 006 will drive the lifting plates 605 on both sides to press down at the same time. When the opening at the bottom end of the lifting plate 605 is pressed downward, it can directly press the adjustment block 330 on the side of the rotating shaft 310 to tilt the entire eight-tube 003. At the same time, the moving plate will stop at a fixed point according to the position of the eight-tube 003, and the injection tube 005 will inject the sample liquid inside it into the slightly tilted eight-tube 003 one by one until the sample in the injection tube 005 is used up.

[0050] Both ends of the eight-tube 003 are fixedly connected with a rotating shaft 310, and the outer side of the rotating shaft 310 is snap-connected with a snap-in block 320, and the snap-in block 320 is rotatably connected to the sample stage 002, and the rear end side of the snap-in block 320 is fixedly connected with an adjustment block 330.

[0051] The bottom end of the lifting plate 605 is configured to be arc-shaped, and the bottom end opening of the lifting plate 605 faces downward. The position of the lifting plate 605 corresponds to the position of the adjustment block 330 .

[0052] When the movable seat 411 moves to the top of the eight-tube 003, the bottom end of the movable seat 411 will directly press the connecting rod 601, so that the connecting rod 601 drives the lifting rod 602 and the lifting plate 605 to press down at the same time, and in the process of pressing down, the bottom opening of the lifting plate 605 will directly contact the adjusting block 330, so that the adjusting block 330 rotates along the arc-shaped opening at the bottom end of the lifting plate 605 during the pressing process, and then the entire eight-tube 003 begins to tilt slightly in one direction, waiting for the injection tube 005 to inject liquid into the eight-tube 003. The tilted eight-tube 003 can effectively make the sample liquid flow downward along the inside of the eight-tube 003, and prevent the sample liquid from splashing out in the test tube, thereby protecting the experimenter and the experimental environment.

[0053] Since both ends of the connecting plate 603 are inclined, when the moving seat 411 leaves the injection range of the eight-tube 003, the connecting plate 603 gradually moves upward under the action of the reset spring 604, and the eight-tube 003 after the injection will return to a vertical state under the action of its own gravity and the lifting plate 605, while preventing the reset spring 604 from driving the lifting rod 602 to rise too fast, causing the eight-tube 003 to shake and causing the sample liquid to splash.

[0054] A transmission screw 410 is rotatably connected to the upper side of the fixed platform 004, and a rotating rod 420 is arranged above the transmission screw 410. Both ends of the transmission screw 410 and the rotating rod 420 are fixedly connected with an adjustment plate 430. The outer wall of the adjustment plate 430 is provided with a plurality of transmission grooves 440, and the plurality of transmission grooves 440 are distributed in a circular array about the central axis of the adjustment plate 430. A transmission plate 450 is transmission-connected between the two adjustment plates 430, and a transmission block 460 is fixedly connected to the bottom end of the transmission plate 450, and the transmission block 460 is arranged inside the transmission groove 440. The two adjustment plates 430 are symmetrically distributed about the central axis of the transmission plate 450, and a servo motor 470 is installed at the rear end of the adjustment plate 430.

[0055] The outer wall of the transmission screw 410 is threadedly connected with a moving seat 411 , and a sliding rod 412 is slidably connected below the moving seat 411 , and the sliding rod 412 is fixedly connected to the fixed platform 004 .

[0056] When the servo motor 470 drives the transmission plate 450 to rotate, the transmission plate 450 is stuck in the transmission slot 440 through the transmission block 460, driving the adjustment plate 430 to rotate. Since the two adjustment plates 430 are symmetrically arranged, when one of the adjustment plates 430 rotates driven by the transmission plate 450, the other adjustment plate 430 will be in a stationary state, so that the two adjustment plates 430 can respectively drive the transmission screw 410 and the rotating rod 420 to rotate at a fixed point.

[0057] At the same time, when the transmission screw 410 rotates, the movable seat 411 threadedly connected to the transmission screw 410 will slide along the slide rod 412. At the same time, each time the transmission screw 410 stops, the movable seat 411 will stop directly above the eight-tube 003, thereby ensuring that the injection tube 005 can be aligned with the eight-tube 003, thereby improving the injection matching degree of the device.

[0058] The outer wall of the rotating rod 420 is provided with a limiting groove 421, and the rotating rod 420 is slidably connected to the worm wheel 422 through the limiting groove 421. A sliding member 423 is provided on the side of the worm wheel 422, and the sliding member 423 is rotatably connected to the movable seat 411. The side of the worm wheel 422 is meshingly connected with a worm 424, and the bottom end of the worm 424 is fixedly connected to a movable rod 425. The outer wall of the movable rod 425 is provided with two movable grooves 426, and the two movable grooves 426 are cross-arranged. The inside of the movable groove 426 is slidably connected with a slider 427, and the front end of the slider 427 is movably connected to a lifting seat 428, and the front end of the lifting seat 428 is fixedly connected to the injection tube 005.

[0059] When the transmission plate 450 drives the adjustment plate 430 on the side of the rotating rod 420 to rotate through the transmission block 460, the adjustment plate 430 can rotate the rotating rod 420 and the worm gear 422 slidably connected thereto, so that the worm gear 422 drives the worm 424 and the movable rod 425 on its side. When the movable rod 425 rotates, the slider 427 drives the lifting seat 428 along the two moving grooves 426 to complete the descending and ascending actions.

[0060] The bottom end of the injection tube 005 is fixedly connected to the first fixed cylinder 510, the outer wall of the first fixed cylinder 510 is slidably connected to the second fixed cylinder 520, the second fixed cylinder 520 is fixedly connected to the movable seat 411, the bottom end of the first fixed cylinder 510 is slidably connected to the injection tube 540, the inlet of the first fixed cylinder 510 is provided with a first one-way valve 530, the outlet of the injection tube 540 is provided with a second one-way valve 550, and the bottom end of the injection tube 540 is inclined.

[0061] Among them, when the lifting seat 428 drives the first fixed cylinder 510 and the injection tube 540 to descend, until the injection tube 540 reaches the bottom of the second fixed cylinder 520, the first fixed cylinder 510 is compressed inside the injection tube 540, and when the first fixed cylinder 510 rises, the liquid sample inside the injection tube 005 is forced to enter the second fixed cylinder 520 through the first one-way valve 530, so that each time the first fixed cylinder 510 descends, the same amount of sample liquid inside the first fixed cylinder 510 will be pressed into the eight-tube 003, so as to ensure that the samples in the eight-tube 003 are equal, and the influence of the test conditions on the samples is consistent, thereby reducing the error introduced by different sample amounts and improving the accuracy of the experimental results;

[0062] At the same time, the inclination direction of the bottom end of the injection tube 540 is opposite to the inclination direction of the eight-tube strip 003. When the eight-tube strip 003 is inclined, the injection tube 540 descends directly to the inner wall of the eight-tube strip 003, so that the sample liquid can slowly flow into the bottom of the eight-tube strip 003.

[0063] Embodiment 2

[0064] like Figure 11-13 In the second embodiment, other structures remain unchanged. What is different from the first embodiment is that the transmission assembly 007 includes a pressing rod 701, the outer wall of the pressing rod 701 is fixedly connected with a sliding block 702, the outer wall of the sliding block 702 is slidably connected with a sliding shell 703, the inner wall of the pressing rod 701 is provided with a positioning block 704, the inner part of the pressing rod 701 is slidably connected with an adjusting rod 705, the outer wall of the adjusting rod 705 begins to have an inclined groove 706, the end of the inclined groove 706 is provided with a vertical groove 707, the bottom end of the adjusting rod 705 is fixedly connected with an adjusting gear 708, the bottom end of the adjusting gear 708 is transmission-connected with a transmission tooth plate 709, the transmission tooth plate 709 is fixedly connected to the sample platform, and the bottom end of the sliding block 702 is installed with a pressure sensor 710.

[0065] The depth of the vertical groove 707 is deeper than that of the inclined groove 706 . The positioning block 704 is embedded in the inclined groove 706 . The bottom end of the positioning block 704 is fixedly connected with a connecting spring 720 . The positioning block 704 is movably connected to the positioning block 704 via the connecting spring 720 .

[0066] When the movable seat 411 moves to the two sides of the fixed platform 004 driven by the transmission screw 410, the movable seat 411 will directly touch the pressing rod 701, and at the same time drive the pressing rod 701 to move backward inside the fixed shell through the sliding block 702, and then the positioning block 704 in the pressing rod 701 slides in the inclined groove 706, and while sliding, drives the adjusting rod 705 and the adjusting gear 708 to rotate half a circle, so that the adjusting gear 708 drives the transmission gear plate 709 to move, and in the process of moving, the already After the injection, the eight-tube strip 003 is sent into the main body 001, and the next row of eight-tube strips 003 is sent to the bottom of the injection tube 005. At the same time, after the moving seat 411 presses the pressing rod 701, the sliding block 702 will slide to the bottom of the sliding shell 703, so that the pressure sensor 710 at the bottom of the sliding block 702 will be pressed by the sliding shell 703, and then the pressure sensor 710 starts the servo motor 470, so that the servo motor 470 starts to reverse, driving the moving seat 411 to move in the opposite direction, so as to facilitate the use of the injection tube 005;

[0067] The sliding shell 703 is slidably connected to the main body 001 , a fixing rod 730 is fixedly connected to the top of the sliding shell 703 , a fixing nut 740 is threadedly connected to the outer wall of the fixing rod 730 , and the fixing nut 740 is rotatably connected to the main body 001 .

[0068] Among them, after the staff moves the sample stage 002 out of the main body 001, they can rotate the fixing nut 740, and when the fixing nut 740 rotates, it will drive the fixing rod 730 to move the sliding shell 703 and the transmission gear inside it out, until the transmission gear is meshed and connected with the transmission gear plate 709. At the same time, the position of the sample stage 002 is further adjusted to correspond to the bottom of the injection tube 005. When the transmission gear is connected to the transmission gear plate 709, the sample stage 002 can only move inward on the main body 001, thereby preventing accidents from happening during the test and facilitating the automatic retraction and release of the sample platform.

[0069] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0070] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. PCR reaction device, comprising: Subject(001); It is characterized by: also including: A sample stage (002), the sample stage (002) is arranged inside the main body (001), and the sample stage (002) can be used to place a sample liquid; An eight-tube joint (003), wherein a plurality of the eight-tube joints (003) are provided, and the plurality of the eight-tube joints (003) are equidistantly arranged inside the sample stage (002), and the eight-tube joints (003) are used to place test samples; A fixed platform (004), wherein the fixed platform (004) is arranged above the sample platform (002); An injection tube (005), the injection tube (005) is arranged at the front end of the fixed platform (004), and the injection tube (005) is used for quantitatively injecting the sample liquid; A tilting assembly (006), wherein the tilting assembly (006) is arranged inside the sample platform (002), and the tilting assembly (006) is used to tilt the eight-tube joint (003) one by one according to the position of the sample, and the tilting assembly (006) comprises a connecting rod (601), and lifting rods (602) are passed through both sides of the connecting rod (601), and the lifting rods (602) are slidably connected to the fixed platform (004), and the top ends of the two lifting rods (602) are fixedly connected to a connecting plate (603), and the two ends of the connecting plate (603) are arranged in an inclined manner, and the outer walls of the two lifting rods (602) are provided with a reset spring (604), and the two ends of the connecting rod (601) are fixedly connected to a lifting plate (605), and the lifting plate (605) is slidably connected to the fixed platform (004), and the lifting plate (605) is arranged to be bent, and the bottom end of the lifting plate (605) is arranged to open downward; A transmission assembly (007), wherein two transmission assemblies (007) are provided, and the two transmission assemblies (007) are symmetrically arranged about the central axis of the fixed platform (004), and the transmission assembly (007) is arranged inside the fixed platform (004), and the transmission assembly (007) is used to push the eight-linked tube (003) at equal intervals.

2. The PCR reaction device according to claim 1, characterized in that: Both ends of the eight-tube (003) are fixedly connected to a rotating shaft (310), the outer side of the rotating shaft (310) is snap-connected to a snap-fit ​​block (320), the snap-fit ​​block (320) is rotatably connected to the sample stage (002), and the rear end side of the snap-fit ​​block (320) is fixedly connected to an adjustment block (330).

3. The PCR reaction device according to claim 2, characterized in that: The bottom end of the lifting plate (605) is configured to be arc-shaped, the bottom end opening of the lifting plate (605) faces downward, and the position of the lifting plate (605) corresponds to the position of the adjustment block (330).

4. The PCR reaction device according to claim 3, characterized in that: A transmission screw (410) is rotatably connected to the upper side of the fixed platform (004), a rotating rod (420) is arranged above the transmission screw (410), and both ends of the transmission screw (410) and the rotating rod (420) are fixedly connected to an adjustment plate (430), and a plurality of transmission grooves (440) are opened on the outer wall of the adjustment plate (430), and the plurality of transmission grooves (440) are distributed in a ring array about the central axis of the adjustment plate (430), and a transmission plate (450) is transmission-connected between the two adjustment plates (430), and a transmission block (460) is fixedly connected to the bottom end of the transmission plate (450), and the transmission block (460) is arranged inside the transmission groove (440), and the two adjustment plates (430) are symmetrically distributed about the central axis of the transmission plate (450), and a servo motor (470) is installed at the rear end of the adjustment plate (430).

5. The PCR reaction device according to claim 4, characterized in that: The outer wall of the transmission screw rod (410) is threadedly connected to a moving seat (411), and a sliding rod (412) is slidably connected below the moving seat (411), and the sliding rod (412) is fixedly connected to the fixed platform (004).

6. The PCR reaction device according to claim 1, characterized in that: The outer wall of the rotating rod (420) is provided with a limiting groove (421), and the rotating rod (420) is slidably connected to a worm wheel (422) through the limiting groove (421). A sliding member (423) is provided on the side of the worm wheel (422), and the sliding member (423) is rotatably connected to the movable seat (411). The side of the worm wheel (422) is meshingly connected with a worm (424), and the bottom end of the worm (424) is fixedly connected to a movable rod (425). The outer wall of the movable rod (425) is provided with two movable grooves (426), and the two movable grooves (426) are cross-arranged. A slider (427) is slidably connected inside the movable groove (426), and the front end of the slider (427) is movably connected to a lifting seat (428), and the front end of the lifting seat (428) is fixedly connected to the injection pipe (005).

7. The PCR reaction device according to claim 1, characterized in that: The bottom end of the injection tube (005) is fixedly connected to a first fixed cylinder (510), the outer wall of the first fixed cylinder (510) is slidably connected to a second fixed cylinder (520), the second fixed cylinder (520) is fixedly connected to a movable seat (411), the bottom end of the first fixed cylinder (510) is slidably connected to an injection tube (540), a first one-way valve (530) is provided at the inlet of the first fixed cylinder (510), a second one-way valve (550) is provided at the outlet of the injection tube (540), and the bottom end of the injection tube (540) is inclined.

8. The PCR reaction device according to claim 1, characterized in that: The transmission assembly (007) includes a pressing rod (701), the outer wall of the pressing rod (701) is fixedly connected with a sliding block (702), the outer wall of the sliding block (702) is slidably connected with a sliding shell (703), the inner wall of the pressing rod (701) is provided with a positioning block (704), the inside of the pressing rod (701) is slidably connected with an adjusting rod (705), the outer wall of the adjusting rod (705) begins to have an inclined groove (706), the end of the inclined groove (706) is provided with a vertical groove (707), the bottom end of the adjusting rod (705) is fixedly connected with an adjusting gear (708), the bottom end of the adjusting gear (708) is transmission-connected with a transmission tooth plate (709), the transmission tooth plate (709) is fixedly connected to the sample platform, and the bottom end of the sliding block (702) is installed with a pressure sensor (710).

9. The PCR reaction device according to claim 8, characterized in that: The depth of the vertical groove (707) is deeper than that of the inclined groove (706); the positioning block (704) is embedded in the inclined groove (706); a connecting spring (720) is fixedly connected to the bottom end of the positioning block (704); and the positioning block (704) is movably connected to the positioning block (704) via the connecting spring (720).

10. The PCR reaction device according to claim 9, characterized in that: The sliding shell (703) is slidably connected to the main body (001), the top end of the sliding shell (703) is fixedly connected to a fixing rod (730), the outer wall of the fixing rod (730) is threadedly connected to a fixing nut (740), and the fixing nut (740) is rotatably connected to the main body (001).

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